Lag0020888 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0020888
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	RING-type E3 ubiquitin transferase
Location	chr7: 2846723 .. 2847979 (-)
RNA-Seq Expression	Lag0020888
Synteny	Lag0020888

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCCGATTTTCCTCCTCTTTTCCGATGCCCCATTTCCATGGAGCTCATGGAGGATCCGGTCACCATCTCCACCGGCGTCACCTACGAGAGAAAAAGCATCGAAAAATGGCTTTTCACTTACAACAACAAAACCTGTCCGGCGACGATGCAGACTCTCGACGCCGCCGGCGAGGATTTCGTCATCACTCCTAATCTCAATCTCAAACGCCTCATTCTCGCTTGGAAGGTCACTGATTCTTCATCGGCGCCGGCGCCGGCGATCGAGGAAGTCCGATCGCTTCTCGCGACTGTCGAGTCTTCTCCGTTTAAGGTGAGTTCGTTGAGGAAATTGAGATCGATGATCGAAATGAGCGATGAGATGAAATCGGAGTTTATTCGATCGAATGGGATCGGGATTGTTGTGAATGTTGTAGCTCAAATTCTTGTTGATTGCTCTGATTTTTCGACGTTTGAAGCTTGTGAGGAGGCTTTGGGAGTGTTGTCTCAGTTTCCAATTTCGAACGAACACAAACTGTTCGACGAAATGTCTAAGAGAGAAGAATCGATGAAATCCATCTCTATTGTTCTTCAAAGAGGAAGTGCAGAAGGGCGTTTCTACGCTGTGAAAATCCTCAGAGATATTGCCAAGAACACCGGTTTCAATTGGGGCTCTGTTTTGGAAGAACAGGGGATTGATTTCTTCAAGCCATTGTTGGAGCTTGTATCAGACGAATTCCCCACCAAGGCTAGCTCGTGCGCATTGGATGTAATGATGGAGATCTTGGCGTCCTCGAAGAGGAGTCGATCGAGGGCGATCGAGGCCGGTGCCGTGTGCGTCCTCATCGAGCTGTTACCGGAGTCGGGGCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCAGAGTTTGTGTGAATGTGCAGAGGGGAGATTGGCTTTGGTGGAACATGGGATGGGAATTGCAGCTGTGACTAAGAAGATTTTGAATGTTTCAAATGGAGCAACAAAATTGGGAGTGAAGATTTTGCTTTGGATTAGTAGTTTTCACCCCAAGGAGAGGGTTGTGGAAGAGATGATGGCTTGTGGGGCAGTGAAGAAGCTGCTGATGATACTGCATATGGACAGCGGCGGCGGCGGCATCGGGGACGGTGGCGGCCGGAGCTCGACGAAGGAGAAGGTGATCAAGATTTTGAAGATGCATGGGAGTTCATGGAGGCGTAGCCCTTGTTTTCCTAGTGAGTTGAAGAATTATTTGAAGTTTGTGAATGATGAATGA

mRNA sequence

ATGGATTCCGATTTTCCTCCTCTTTTCCGATGCCCCATTTCCATGGAGCTCATGGAGGATCCGGTCACCATCTCCACCGGCGTCACCTACGAGAGAAAAAGCATCGAAAAATGGCTTTTCACTTACAACAACAAAACCTGTCCGGCGACGATGCAGACTCTCGACGCCGCCGGCGAGGATTTCGTCATCACTCCTAATCTCAATCTCAAACGCCTCATTCTCGCTTGGAAGGTCACTGATTCTTCATCGGCGCCGGCGCCGGCGATCGAGGAAGTCCGATCGCTTCTCGCGACTGTCGAGTCTTCTCCGTTTAAGGTGAGTTCGTTGAGGAAATTGAGATCGATGATCGAAATGAGCGATGAGATGAAATCGGAGTTTATTCGATCGAATGGGATCGGGATTGTTGTGAATGTTGTAGCTCAAATTCTTGTTGATTGCTCTGATTTTTCGACGTTTGAAGCTTGTGAGGAGGCTTTGGGAGTGTTGTCTCAGTTTCCAATTTCGAACGAACACAAACTGTTCGACGAAATGTCTAAGAGAGAAGAATCGATGAAATCCATCTCTATTGTTCTTCAAAGAGGAAGTGCAGAAGGGCGTTTCTACGCTGTGAAAATCCTCAGAGATATTGCCAAGAACACCGGTTTCAATTGGGGCTCTGTTTTGGAAGAACAGGGGATTGATTTCTTCAAGCCATTGTTGGAGCTTGTATCAGACGAATTCCCCACCAAGGCTAGCTCGTGCGCATTGGATGTAATGATGGAGATCTTGGCGTCCTCGAAGAGGAGTCGATCGAGGGCGATCGAGGCCGGTGCCGTGTGCGTCCTCATCGAGCTGTTACCGGAGTCGGGGCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCAGAGTTTGTGTGAATGTGCAGAGGGGAGATTGGCTTTGGTGGAACATGGGATGGGAATTGCAGCTGTGACTAAGAAGATTTTGAATGTTTCAAATGGAGCAACAAAATTGGGAGTGAAGATTTTGCTTTGGATTAGTAGTTTTCACCCCAAGGAGAGGGTTGTGGAAGAGATGATGGCTTGTGGGGCAGTGAAGAAGCTGCTGATGATACTGCATATGGACAGCGGCGGCGGCGGCATCGGGGACGGTGGCGGCCGGAGCTCGACGAAGGAGAAGGTGATCAAGATTTTGAAGATGCATGGGAGTTCATGGAGGCGTAGCCCTTGTTTTCCTAGTGAGTTGAAGAATTATTTGAAGTTTGTGAATGATGAATGA

Coding sequence (CDS)

ATGGATTCCGATTTTCCTCCTCTTTTCCGATGCCCCATTTCCATGGAGCTCATGGAGGATCCGGTCACCATCTCCACCGGCGTCACCTACGAGAGAAAAAGCATCGAAAAATGGCTTTTCACTTACAACAACAAAACCTGTCCGGCGACGATGCAGACTCTCGACGCCGCCGGCGAGGATTTCGTCATCACTCCTAATCTCAATCTCAAACGCCTCATTCTCGCTTGGAAGGTCACTGATTCTTCATCGGCGCCGGCGCCGGCGATCGAGGAAGTCCGATCGCTTCTCGCGACTGTCGAGTCTTCTCCGTTTAAGGTGAGTTCGTTGAGGAAATTGAGATCGATGATCGAAATGAGCGATGAGATGAAATCGGAGTTTATTCGATCGAATGGGATCGGGATTGTTGTGAATGTTGTAGCTCAAATTCTTGTTGATTGCTCTGATTTTTCGACGTTTGAAGCTTGTGAGGAGGCTTTGGGAGTGTTGTCTCAGTTTCCAATTTCGAACGAACACAAACTGTTCGACGAAATGTCTAAGAGAGAAGAATCGATGAAATCCATCTCTATTGTTCTTCAAAGAGGAAGTGCAGAAGGGCGTTTCTACGCTGTGAAAATCCTCAGAGATATTGCCAAGAACACCGGTTTCAATTGGGGCTCTGTTTTGGAAGAACAGGGGATTGATTTCTTCAAGCCATTGTTGGAGCTTGTATCAGACGAATTCCCCACCAAGGCTAGCTCGTGCGCATTGGATGTAATGATGGAGATCTTGGCGTCCTCGAAGAGGAGTCGATCGAGGGCGATCGAGGCCGGTGCCGTGTGCGTCCTCATCGAGCTGTTACCGGAGTCGGGGCGATCGAAATGCGAGCGAATGCTGCAGATTCTGCAGAGTTTGTGTGAATGTGCAGAGGGGAGATTGGCTTTGGTGGAACATGGGATGGGAATTGCAGCTGTGACTAAGAAGATTTTGAATGTTTCAAATGGAGCAACAAAATTGGGAGTGAAGATTTTGCTTTGGATTAGTAGTTTTCACCCCAAGGAGAGGGTTGTGGAAGAGATGATGGCTTGTGGGGCAGTGAAGAAGCTGCTGATGATACTGCATATGGACAGCGGCGGCGGCGGCATCGGGGACGGTGGCGGCCGGAGCTCGACGAAGGAGAAGGTGATCAAGATTTTGAAGATGCATGGGAGTTCATGGAGGCGTAGCCCTTGTTTTCCTAGTGAGTTGAAGAATTATTTGAAGTTTGTGAATGATGAATGA

Protein sequence

MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGEDFVITPNLNLKRLILAWKVTDSSSAPAPAIEEVRSLLATVESSPFKVSSLRKLRSMIEMSDEMKSEFIRSNGIGIVVNVVAQILVDCSDFSTFEACEEALGVLSQFPISNEHKLFDEMSKREESMKSISIVLQRGSAEGRFYAVKILRDIAKNTGFNWGSVLEEQGIDFFKPLLELVSDEFPTKASSCALDVMMEILASSKRSRSRAIEAGAVCVLIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNGATKLGVKILLWISSFHPKERVVEEMMACGAVKKLLMILHMDSGGGGIGDGGGRSSTKEKVIKILKMHGSSWRRSPCFPSELKNYLKFVNDE

Homology

[Close]

BLAST of Lag0020888 vs. NCBI nr
Match: XP_038902365.1 (E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida])

HSP 1 Score: 734.2 bits (1894), Expect = 6.4e-208
Identity = 375/418 (89.71%), Postives = 396/418 (94.74%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGED 60
           M+SDFPPLFRCPISMELMEDPVT+STGVTY+RKSIEKWLFTYN KTCPATMQTL A  ED
Sbjct: 1   MESDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLSAGDED 60

Query: 61  FVITPNLNLKRLILAWKVTDSSSAPAPAIEEVRSLLATVESSPFKVSSLRKLRSMIEMSD 120
           FVITPNL LKRLILAWK+TDSSS+PA A+EEVRSLLATVESSPFKVSSLRKLRSMIEMSD
Sbjct: 61  FVITPNLTLKRLILAWKITDSSSSPATALEEVRSLLATVESSPFKVSSLRKLRSMIEMSD 120

Query: 121 EMKSEFIRSNGIGIVVNVVAQILVDCSDFSTFEACEEALGVLSQFPISNEHKLFDEMSKR 180
            MKSEFIRSNGIGIVVNVV QIL+DCSDFST E CEEALGVL QFPIS+E KLFDEMSKR
Sbjct: 121 GMKSEFIRSNGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISDEDKLFDEMSKR 180

Query: 181 EESMKSISIVLQRGSAEGRFYAVKILRDIAKNTGFNWGSVLEEQGIDFFKPLLELVSDEF 240
           EE MKS++IVLQRGSAEGRFYAVKILR+IAKNT +NWGSV+EEQGIDFFKPLLELVSDEF
Sbjct: 181 EELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQGIDFFKPLLELVSDEF 240

Query: 241 PTKASSCALDVMMEILASSKRSRSRAIEAGAVCVLIELLPESGRSKCERMLQILQSLCEC 300
            TKASSCALDVM+EILASSKRSR +AIEAGAV +LIELLPESGRSKCERMLQILQSLCEC
Sbjct: 241 ATKASSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSNGATKLGVKILLWISSFHPKERVVEEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVSN ATK+GVKILLWISSFHP+ERVVEEMMACGAVKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPRERVVEEMMACGAVKK 360

Query: 361 LLMILHMDSGGGGIGDGGGRSSTKEKVIKILKMHGSSWRRSPCFPSELKNYLKFVNDE 419
           LLMILHMDS   G+GD GGRSSTKEKVIKILKMHG+SWRRSPCFPSELKNYLKFVND+
Sbjct: 361 LLMILHMDS--VGVGD-GGRSSTKEKVIKILKMHGNSWRRSPCFPSELKNYLKFVNDD 415

[Close]

BLAST of Lag0020888 vs. NCBI nr
Match: KAG7028702.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 727.2 bits (1876), Expect = 7.8e-206
Identity = 379/418 (90.67%), Postives = 391/418 (93.54%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGED 60
           MDSDFPPLF CPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTL AAG D
Sbjct: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTL-AAG-D 60

Query: 61  FVITPNLNLKRLILAWKVTDSSSAPAPAIEEVRSLLATVESSPFKVSSLRKLRSMIEMSD 120
           FVITPNL LKRLILAWK+TDSS++PA  IEEVRSLLATVESSPFKVSSLRKLRSMIE SD
Sbjct: 61  FVITPNLTLKRLILAWKITDSSASPA-TIEEVRSLLATVESSPFKVSSLRKLRSMIETSD 120

Query: 121 EMKSEFIRSNGIGIVVNVVAQILVDCSDFSTFEACEEALGVLSQFPISNEHKLFDEMSKR 180
           EMKSEF+R NGIGIVVNVV QIL+DCSDF TFEACEEA+GVLSQ PISNE KLFDEMSKR
Sbjct: 121 EMKSEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKR 180

Query: 181 EESMKSISIVLQRGSAEGRFYAVKILRDIAKNTGFNWGSVLEEQGIDFFKPLLELVSDEF 240
           EE MKSI+IVLQRGSAEGRFYAVKILR+IAKNT FNWGSVLEEQGIDFFKPLLELVSDEF
Sbjct: 181 EELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEF 240

Query: 241 PTKASSCALDVMMEILASSKRSRSRAIEAGAVCVLIELLPESGRSKCERMLQILQSLCEC 300
            TKASSCALDVMMEILA SKRSR +AIEAGAVC+LIELLPESGRSKCERMLQILQSLCEC
Sbjct: 241 STKASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSNGATKLGVKILLWISSFHPKERVVEEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVSN ATK+GVKILLWISSFHP ERVVEEMMACG VKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKK 360

Query: 361 LLMILHMDSGGGGIGDGGGRSSTKEKVIKILKMHGSSWRRSPCFPSELKNYLKFVNDE 419
           L+MILHMD GGG  G GGGRSSTKEK IKILKMHGSSWRRSPCFPSELKNYL FVN E
Sbjct: 361 LMMILHMDGGGG--GGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 413

[Close]

BLAST of Lag0020888 vs. NCBI nr
Match: XP_022959873.1 (E3 ubiquitin-protein ligase PUB23-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 725.7 bits (1872), Expect = 2.3e-205
Identity = 378/418 (90.43%), Postives = 390/418 (93.30%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGED 60
           MDSDFPPLF CPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTL AAG D
Sbjct: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTL-AAG-D 60

Query: 61  FVITPNLNLKRLILAWKVTDSSSAPAPAIEEVRSLLATVESSPFKVSSLRKLRSMIEMSD 120
           FVITPNL LKRLILAWK+TDSS++PA  IEEVRSLLATVESSPFKVSSLRKLRSMIE SD
Sbjct: 61  FVITPNLTLKRLILAWKITDSSASPA-TIEEVRSLLATVESSPFKVSSLRKLRSMIETSD 120

Query: 121 EMKSEFIRSNGIGIVVNVVAQILVDCSDFSTFEACEEALGVLSQFPISNEHKLFDEMSKR 180
           EMKSEF+R NGIGIVVNVV QIL+DCSDF TFEACEEA+GVLSQ PISNE KLFDEMSKR
Sbjct: 121 EMKSEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKR 180

Query: 181 EESMKSISIVLQRGSAEGRFYAVKILRDIAKNTGFNWGSVLEEQGIDFFKPLLELVSDEF 240
           EE MKSI+IVLQRGSAEGRFYAVKILR+IAKNT FNWGSVLEEQGIDFFKPLLELVSDEF
Sbjct: 181 EELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEF 240

Query: 241 PTKASSCALDVMMEILASSKRSRSRAIEAGAVCVLIELLPESGRSKCERMLQILQSLCEC 300
            TKASSC LDVMMEILA SKRSR +AIEAGAVC+LIELLPESGRSKCERMLQILQSLCEC
Sbjct: 241 STKASSCTLDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSNGATKLGVKILLWISSFHPKERVVEEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVSN ATK+GVKILLWISSFHP ERVVEEMMACG VKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKK 360

Query: 361 LLMILHMDSGGGGIGDGGGRSSTKEKVIKILKMHGSSWRRSPCFPSELKNYLKFVNDE 419
           L+MILHMD GGG  G GGGRSSTKEK IKILKMHGSSWRRSPCFPSELKNYL FVN E
Sbjct: 361 LMMILHMDGGGG--GGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 413

[Close]

BLAST of Lag0020888 vs. NCBI nr
Match: KAG6597231.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 725.3 bits (1871), Expect = 3.0e-205
Identity = 378/418 (90.43%), Postives = 390/418 (93.30%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGED 60
           MDSDFPPLF CPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTL AAG D
Sbjct: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTL-AAG-D 60

Query: 61  FVITPNLNLKRLILAWKVTDSSSAPAPAIEEVRSLLATVESSPFKVSSLRKLRSMIEMSD 120
           FVITPNL LKRLILAWK+TDSS++PA  IEEVRSLLATVESSPFKVSSLRKLRSMIE SD
Sbjct: 61  FVITPNLTLKRLILAWKITDSSASPA-TIEEVRSLLATVESSPFKVSSLRKLRSMIETSD 120

Query: 121 EMKSEFIRSNGIGIVVNVVAQILVDCSDFSTFEACEEALGVLSQFPISNEHKLFDEMSKR 180
           EMKSEF+R NGIGIVVNVV QIL+DCSDF TFEACEEA+GVLSQ PISNE KLFDEMSKR
Sbjct: 121 EMKSEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKR 180

Query: 181 EESMKSISIVLQRGSAEGRFYAVKILRDIAKNTGFNWGSVLEEQGIDFFKPLLELVSDEF 240
           EE MKSI+IVLQRGSAEGRFYAVKILR+IAKNT FNWGSVLEEQGIDFFKPLLELVSDEF
Sbjct: 181 EELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEF 240

Query: 241 PTKASSCALDVMMEILASSKRSRSRAIEAGAVCVLIELLPESGRSKCERMLQILQSLCEC 300
            TKASSCALDVMMEILA SKRSR +AIEAGAVC+LIELLPESGRSKCERMLQILQSLCEC
Sbjct: 241 STKASSCALDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSNGATKLGVKILLWISSFHPKERVVEEMMACGAVKK 360
           AEGRL LVEHGMGIAAVTKKILNVSN ATK+GVKILLWISSFHP ERVVEEMMACG VKK
Sbjct: 301 AEGRLPLVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKK 360

Query: 361 LLMILHMDSGGGGIGDGGGRSSTKEKVIKILKMHGSSWRRSPCFPSELKNYLKFVNDE 419
           L+MILHMD GGG  G GGGRSSTKEK IKILKMHGSSWRRSPCFPSELKNYL FVN E
Sbjct: 361 LMMILHMDGGGG--GGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 413

[Close]

BLAST of Lag0020888 vs. NCBI nr
Match: XP_004134357.1 (E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN56649.1 hypothetical protein Csa_010227 [Cucumis sativus])

HSP 1 Score: 718.4 bits (1853), Expect = 3.6e-203
Identity = 364/418 (87.08%), Postives = 390/418 (93.30%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGED 60
           MDSDFPPLFRCPISMELMEDPVT+STGVTY+RKSIEKWLFTYN KTCPATMQTL A  ED
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60

Query: 61  FVITPNLNLKRLILAWKVTDSSSAPAPAIEEVRSLLATVESSPFKVSSLRKLRSMIEMSD 120
           FVITPNLNLKRLILAWK+ DS+  PA A+EEV SLLATVESSPFKVSSLRKLRSM+EM+D
Sbjct: 61  FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND 120

Query: 121 EMKSEFIRSNGIGIVVNVVAQILVDCSDFSTFEACEEALGVLSQFPISNEHKLFDEMSKR 180
            MKSEFIR +GIGIVVNVV QIL+DCSDFST + CEEALGVL QFPISNE KLFDEM KR
Sbjct: 121 GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQFPISNEDKLFDEMYKR 180

Query: 181 EESMKSISIVLQRGSAEGRFYAVKILRDIAKNTGFNWGSVLEEQGIDFFKPLLELVSDEF 240
           EE MKSI++V+QRGSAEGRFYAVKILR+IAKNT +NWGS++EEQGIDFFKPLLELVSDEF
Sbjct: 181 EELMKSIAVVVQRGSAEGRFYAVKILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEF 240

Query: 241 PTKASSCALDVMMEILASSKRSRSRAIEAGAVCVLIELLPESGRSKCERMLQILQSLCEC 300
           PTKASSCALDVM+EIL+SSKRSR +AIEAGAV +LIELLPESGRSKCERMLQILQSLCEC
Sbjct: 241 PTKASSCALDVMIEILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSNGATKLGVKILLWISSFHPKERVVEEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVS  ATK+GVKILLWISSFHP+ERVV+EMMACGAVKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360

Query: 361 LLMILHMDSGGGGIGDGGGRSSTKEKVIKILKMHGSSWRRSPCFPSELKNYLKFVNDE 419
           LLMILHMDS   G+GD GGRSSTKEKVIKILKMHG+ WRRSPCFPSELKNYLKFVNDE
Sbjct: 361 LLMILHMDS--TGVGD-GGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

[Close]

BLAST of Lag0020888 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 2.1e-52
Identity = 137/420 (32.62%), Postives = 220/420 (52.38%), Query Frame = 0


Query: 4   DFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGEDFVI 63
           + PP F CPIS+E+M+DPV +STG+TY+R SIEKWLF     +CP T Q +     D  +
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDI----TDADL 70

Query: 64  TPNLNLKRLILAWKVTDSS------SAPAPAI--EEVRSLLA-TVESSPFKVSSLRKLRS 123
           TPN  L+RLI +W   ++S        P P I   E+  L+  +  S   +V  L++LR 
Sbjct: 71  TPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQ 130

Query: 124 MIEMSDEMKSEFIRSNGIGIVVNVVAQILVDCSDFSTFEACEEALGVLSQFPISNEHKLF 183
           ++  +   K     +     + N+V+      +D       +EAL +L     S E  L 
Sbjct: 131 IVSENATNKRCLEAAGVPEFLANIVS------NDSENGSLTDEALNLLYHLETS-ETVLK 190

Query: 184 DEMSKREES--MKSISIVLQRGSAEGRFYAVKILRDIAKNTGFNWGSVLEEQGIDFFKPL 243
           + ++ ++++  +KS++ ++QRG  E R YA  +L++I +         L+    + F  +
Sbjct: 191 NLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP---EVFTEV 250

Query: 244 LELVSDEFPTKASSCALDVMMEILASSKRSRSRAIEAGAVCVLIELLPE----SGRSKCE 303
           ++++ D    KA+  A+ +++ I     R+R +A+EAG + V+IELL +    S R   E
Sbjct: 251 VQILDDRISQKATKAAMHILVNICPWG-RNRHKAVEAGVISVIIELLMDESFTSERRGPE 310

Query: 304 RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNGATKLGVKILLWISSFHPKERV 363
             + +L  LC+CAEGR   + HG  IA V KKIL VS  A+   V++LL +  F     +
Sbjct: 311 MAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPAL 370

Query: 364 VEEMMACGAVKKLLMILHMDSGGGGIGDGGGRSSTKEKVIKILKMHGSSWRRSPCFPSEL 409
           + EM+  G V KL ++L +  GG           TKEK  ++LK+H   W+ SPC P  +
Sbjct: 371 LHEMLQLGVVAKLCLVLQVSCGG----------KTKEKAKELLKLHARVWKDSPCLPKNM 405

[Close]

BLAST of Lag0020888 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 6.9e-48
Identity = 134/442 (30.32%), Postives = 218/442 (49.32%), Query Frame = 0


Query: 4   DFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGEDFVI 63
           + P  F CPIS+++M+DPV +STG+TY+R+SIEKWLF+    +CP T Q +        +
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD----L 65

Query: 64  TPNLNLKRLILAWKVTDSS------SAPAPAI--EEVRSLLATVESSPF-KVSSLRKLRS 123
           TPN  L+RLI +W   ++S        P P I   E+  L+    SS   +V  L++LR 
Sbjct: 66  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQ 125

Query: 124 MIEMSDEMKSEFIRSNGIGIVVNVVAQIL-------VDCSDFSTFEAC------------ 183
           ++  +   K     +     + N+V+  +          S  +  + C            
Sbjct: 126 IVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSS 185

Query: 184 ----EEALGVLSQFPISNEH-KLFDEMSKREESMKSISIVLQRGSAEGRFYAVKILRDIA 243
               +EAL VL     S    K      K    +K+++ ++QRG  E R YA  +L+ + 
Sbjct: 186 RSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLL 245

Query: 244 KNTGFNWGSVLEEQGIDFFKPLLELVSDEFPTKASSCALDVMMEILASSKRSRSRAIEAG 303
           +        +LE    + F  +++++ D+   KA+  A+ +++ I     R+R +A+E G
Sbjct: 246 EVADPMQIILLER---ELFGEVIQILHDQISHKATRSAMQILV-ITCPWGRNRHKAVEGG 305

Query: 304 AVCVLIELLPE----SGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSN 363
            + ++IELL +    S R   E  + +L  LC+CAEGR   + HG  IA V+KKIL VS 
Sbjct: 306 TISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQ 365

Query: 364 GATKLGVKILLWISSFHPKERVVEEMMACGAVKKLLMILHMDSGGGGIGDGGGRSSTKEK 409
             ++  V++LL +  F     +++EM+  G V KL ++L +  G          + TKEK
Sbjct: 366 ITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCG----------NKTKEK 425

[Close]

BLAST of Lag0020888 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 2.7e-36
Identity = 135/449 (30.07%), Postives = 214/449 (47.66%), Query Frame = 0


Query: 4   DFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGEDFVI 63
           + P  F CPIS+E+M+DPVT  +G+TY+R++I KWL      +CP T Q L     D  +
Sbjct: 9   EIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPL---DSDL 68

Query: 64  TPNLNLKRLILAWKVTDSS------SAP------APAIEEVRSLLATVESSPFKVSSLRK 123
           TPN  L+RLI  W V + +      S P         +EE+++L    + +  +  +L+K
Sbjct: 69  TPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQK 128

Query: 124 LRSMIEMSDEMKSEFIRSNGI--GIVVNVVAQILVDCSDFSTFEACEEALGVLSQFPI-S 183
           L   +   D      +   G+   +++ VV     D       +  +E+L +L    I S
Sbjct: 129 LE--VLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPS 188

Query: 184 NEHKLFDEMSKREESMKSISIVLQRGSAEGRFYAVKILRDIAKNTGFNWGSVLEEQ---- 243
           N+ K    + + +  M+S++ VL +     + Y + +LR++ + T  +    L  +    
Sbjct: 189 NDAKTI--LMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIFKG 248

Query: 244 GIDFFKPLLELVSDEFPT----------------------------KASSCALDVMMEIL 303
            I F K ++  V+   PT                            +A + AL +++E  
Sbjct: 249 IIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLE-T 308

Query: 304 ASSKRSRSRAIEAGAVCVLIEL-LPESGRSK-CERMLQILQSLCECAEGRLALVEHGMGI 363
           +S  R+RS  ++ GAV  LIEL +  +G  +  E ML +L  LC CA GR  ++ H  GI
Sbjct: 309 SSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGI 368

Query: 364 AAVTKKILNVSNGATKLGVKILLWISSFHPKERVVEEMMACGAVKKLLMILHMDSGGGGI 404
           A VTK++L VS  A    + IL  +S F P+  VVEEM+  G V+KL  +L MD G    
Sbjct: 369 AVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCG---- 428

[Close]

BLAST of Lag0020888 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.2e-33
Identity = 121/411 (29.44%), Postives = 194/411 (47.20%), Query Frame = 0


Query: 6   PPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGEDFVITP 65
           P  FRCPIS+ELM+DPVT+ TG TY+R SIE W+   NN TCP T   L     DF + P
Sbjct: 15  PYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPL----SDFTLIP 74

Query: 66  NLNLKRLILAWKVTDSSSA----PAP-----------AIEEVRSLLATVESSPFKVSSLR 125
           N  L+RLI  W V + S+     P P            + +  ++  T  S   + ++LR
Sbjct: 75  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALR 134

Query: 126 KLRSMIEMSDEMKSEFIRSNGIGIVVNVVAQILVDCSDFSTFEACEEALGVLSQFPISNE 185
           +LR     SD+ +      N   I++ ++       S+ ++ E   E+L +L   PI+ E
Sbjct: 135 RLRGFARDSDKNRVLIAAHNATEILIKIL------FSETTSSELVSESLALLVMLPIT-E 194

Query: 186 HKLFDEMSKREESMKSISIVLQRGSAEGRFYAVKILRDIAKNT--GFNWGSVLEEQGIDF 245
              F  +S     ++ ++ +L   S E R  A  ++  ++  T      GS+   + +  
Sbjct: 195 PNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESV-- 254

Query: 246 FKPLLELVSDEFPTKASSCALDVMMEILASSKRSRSRAIEAGAVCVLIE-LLPESGRSKC 305
           F+ +L+L+ +   ++ +       +  L S K +R  AI AGA  +LI+ L  +  R   
Sbjct: 255 FEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDT 314

Query: 306 ERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNGATKLGVKILLWISSFHPKER 365
           ER L  ++ LC   EG  A  EH + +  + K IL VS+ AT+     LL + +   +ER
Sbjct: 315 ERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCT--AEER 374

Query: 366 VVEEMMACGAVKKLLMILHMDSGGGGIGDGGGRSSTKEKVIKILKMHGSSW 399
             EE    G V +LL+++  +               K+K  K+LK+   SW
Sbjct: 375 WREEAAGAGVVVQLLLMVQSEC----------TERAKKKAQKLLKLLRDSW 400

[Close]

BLAST of Lag0020888 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 3.2e-29
Identity = 121/424 (28.54%), Postives = 197/424 (46.46%), Query Frame = 0


Query: 6   PPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGEDFVITP 65
           PP F+CPIS++LM+DPV ISTG+TY+R SIE W+    NKTCP T   L      F   P
Sbjct: 32  PPEFQCPISIDLMKDPVIISTGITYDRVSIETWI-NSGNKTCPVTNTVLTT----FDQIP 91

Query: 66  NLNLKRLILAWKVTDSSSAPAPAIEEVRSLLATVESSPFKVSSL-RKLRSMIEMSDEMKS 125
           N  ++++I  W V   S    P I+ + +    V   P +V  + RKL S     D  K 
Sbjct: 92  NHTIRKMIQGWCVEKGS----PLIQRIPT--PRVPLMPCEVYEISRKLSSATRRGDYEKC 151

Query: 126 EFI-----------RSNGIGIVVNVVAQILVDCSD-FSTFE----ACEEALGVLS-QFPI 185
             I             N   +  N V  +L DC D FS  E       E L +L+  FPI
Sbjct: 152 GVIIEKIKKLGDESEKNRKCVNENSVGWVLCDCFDKFSGDEKLTFMLNEILSLLTWMFPI 211

Query: 186 SNEHKLFDEMSK--REESMKSISIVLQRGSAEGRFYAVKILRDIAKNTGFNWGSVLEEQG 245
                  + +SK     S + ++ +L+      R  A  I+++I         S   E G
Sbjct: 212 G-----LEGISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENG 271

Query: 246 IDFFKPLLELVSDEFPTKASSCALDVMMEILASSKRSRSRAIEAGAVCVLIELLPESGRS 305
           +   + L++L+ D   + ++  +L  + +++       S  +E G V + +E++ ++  S
Sbjct: 272 V--AEALVKLIRDSVSSSSTKSSLIAIYQMVLQKPEIASEFLEIGLVSITVEMIVDAENS 331

Query: 306 KCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNGATKLGVKIL--LWISSFH 365
            CE+ L +L ++CE   GR  + ++ + +  + KKI  VS  AT+  + ++  LW +   
Sbjct: 332 VCEKALAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTG-- 391

Query: 366 PKERVVEEMMACGAVKKLLMILHMDSGGGGIGDGGGRSSTKEKVIKILKMHGSSWR-RSP 407
                VE+ +  GA +K+L++L +  G            TKEK  ++LKM  +  +  S 
Sbjct: 392 -NTVAVEDAVRLGAFQKVLLVLQVGYG----------EETKEKATELLKMMNTQMKLMSD 424

[Close]

BLAST of Lag0020888 vs. ExPASy TrEMBL
Match: A0A6J1H765 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460728 PE=4 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 1.1e-205
Identity = 378/418 (90.43%), Postives = 390/418 (93.30%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGED 60
           MDSDFPPLF CPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTL AAG D
Sbjct: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTL-AAG-D 60

Query: 61  FVITPNLNLKRLILAWKVTDSSSAPAPAIEEVRSLLATVESSPFKVSSLRKLRSMIEMSD 120
           FVITPNL LKRLILAWK+TDSS++PA  IEEVRSLLATVESSPFKVSSLRKLRSMIE SD
Sbjct: 61  FVITPNLTLKRLILAWKITDSSASPA-TIEEVRSLLATVESSPFKVSSLRKLRSMIETSD 120

Query: 121 EMKSEFIRSNGIGIVVNVVAQILVDCSDFSTFEACEEALGVLSQFPISNEHKLFDEMSKR 180
           EMKSEF+R NGIGIVVNVV QIL+DCSDF TFEACEEA+GVLSQ PISNE KLFDEMSKR
Sbjct: 121 EMKSEFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKR 180

Query: 181 EESMKSISIVLQRGSAEGRFYAVKILRDIAKNTGFNWGSVLEEQGIDFFKPLLELVSDEF 240
           EE MKSI+IVLQRGSAEGRFYAVKILR+IAKNT FNWGSVLEEQGIDFFKPLLELVSDEF
Sbjct: 181 EELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEF 240

Query: 241 PTKASSCALDVMMEILASSKRSRSRAIEAGAVCVLIELLPESGRSKCERMLQILQSLCEC 300
            TKASSC LDVMMEILA SKRSR +AIEAGAVC+LIELLPESGRSKCERMLQILQSLCEC
Sbjct: 241 STKASSCTLDVMMEILALSKRSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSNGATKLGVKILLWISSFHPKERVVEEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVSN ATK+GVKILLWISSFHP ERVVEEMMACG VKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKK 360

Query: 361 LLMILHMDSGGGGIGDGGGRSSTKEKVIKILKMHGSSWRRSPCFPSELKNYLKFVNDE 419
           L+MILHMD GGG  G GGGRSSTKEK IKILKMHGSSWRRSPCFPSELKNYL FVN E
Sbjct: 361 LMMILHMDGGGG--GGGGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 413

[Close]

BLAST of Lag0020888 vs. ExPASy TrEMBL
Match: A0A0A0L6J2 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G127120 PE=4 SV=1)

HSP 1 Score: 718.4 bits (1853), Expect = 1.7e-203
Identity = 364/418 (87.08%), Postives = 390/418 (93.30%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGED 60
           MDSDFPPLFRCPISMELMEDPVT+STGVTY+RKSIEKWLFTYN KTCPATMQTL A  ED
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60

Query: 61  FVITPNLNLKRLILAWKVTDSSSAPAPAIEEVRSLLATVESSPFKVSSLRKLRSMIEMSD 120
           FVITPNLNLKRLILAWK+ DS+  PA A+EEV SLLATVESSPFKVSSLRKLRSM+EM+D
Sbjct: 61  FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND 120

Query: 121 EMKSEFIRSNGIGIVVNVVAQILVDCSDFSTFEACEEALGVLSQFPISNEHKLFDEMSKR 180
            MKSEFIR +GIGIVVNVV QIL+DCSDFST + CEEALGVL QFPISNE KLFDEM KR
Sbjct: 121 GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQFPISNEDKLFDEMYKR 180

Query: 181 EESMKSISIVLQRGSAEGRFYAVKILRDIAKNTGFNWGSVLEEQGIDFFKPLLELVSDEF 240
           EE MKSI++V+QRGSAEGRFYAVKILR+IAKNT +NWGS++EEQGIDFFKPLLELVSDEF
Sbjct: 181 EELMKSIAVVVQRGSAEGRFYAVKILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEF 240

Query: 241 PTKASSCALDVMMEILASSKRSRSRAIEAGAVCVLIELLPESGRSKCERMLQILQSLCEC 300
           PTKASSCALDVM+EIL+SSKRSR +AIEAGAV +LIELLPESGRSKCERMLQILQSLCEC
Sbjct: 241 PTKASSCALDVMIEILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSNGATKLGVKILLWISSFHPKERVVEEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVS  ATK+GVKILLWISSFHP+ERVV+EMMACGAVKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360

Query: 361 LLMILHMDSGGGGIGDGGGRSSTKEKVIKILKMHGSSWRRSPCFPSELKNYLKFVNDE 419
           LLMILHMDS   G+GD GGRSSTKEKVIKILKMHG+ WRRSPCFPSELKNYLKFVNDE
Sbjct: 361 LLMILHMDS--TGVGD-GGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

[Close]

BLAST of Lag0020888 vs. ExPASy TrEMBL
Match: A0A6J1IKT9 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111474953 PE=4 SV=1)

HSP 1 Score: 717.2 bits (1850), Expect = 3.9e-203
Identity = 375/418 (89.71%), Postives = 387/418 (92.58%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGED 60
           MDSDFPPLF CPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTL AAG D
Sbjct: 1   MDSDFPPLFGCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTL-AAG-D 60

Query: 61  FVITPNLNLKRLILAWKVTDSSSAPAPAIEEVRSLLATVESSPFKVSSLRKLRSMIEMSD 120
           FVITPNLNLKRLILAWK+TDSS++PA  IEEVRSLLATVESSPFKVSSLRKLRSMIE SD
Sbjct: 61  FVITPNLNLKRLILAWKITDSSASPA-TIEEVRSLLATVESSPFKVSSLRKLRSMIETSD 120

Query: 121 EMKSEFIRSNGIGIVVNVVAQILVDCSDFSTFEACEEALGVLSQFPISNEHKLFDEMSKR 180
           EMKS F+R NGIGIVVNVV QIL+DCSDF TFEACEEA+GVLSQ PISNE KLFDEMSKR
Sbjct: 121 EMKSGFVRLNGIGIVVNVVVQILIDCSDFWTFEACEEAVGVLSQLPISNEDKLFDEMSKR 180

Query: 181 EESMKSISIVLQRGSAEGRFYAVKILRDIAKNTGFNWGSVLEEQGIDFFKPLLELVSDEF 240
           EE MKSI+IVLQRGSAEGRFYAVKILR+IAKNT FNWGSVLEEQGIDFFKPLLELVSDEF
Sbjct: 181 EELMKSIAIVLQRGSAEGRFYAVKILRNIAKNTNFNWGSVLEEQGIDFFKPLLELVSDEF 240

Query: 241 PTKASSCALDVMMEILASSKRSRSRAIEAGAVCVLIELLPESGRSKCERMLQILQSLCEC 300
            TKASSCALDVMMEILA SK SR +AIEAGAVC+LIELLPESGRSKCERMLQILQSLCEC
Sbjct: 241 STKASSCALDVMMEILALSKSSRVKAIEAGAVCILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSNGATKLGVKILLWISSFHPKERVVEEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVSN ATK+GVKILLWISSFHP ERVVEEMMACG VKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPNERVVEEMMACGVVKK 360

Query: 361 LLMILHMDSGGGGIGDGGGRSSTKEKVIKILKMHGSSWRRSPCFPSELKNYLKFVNDE 419
           L+MILHMD G      GGGRSSTKEK IKILKMHGSSWRRSPCFPSELKNYL FVN E
Sbjct: 361 LMMILHMDGG------GGGRSSTKEKAIKILKMHGSSWRRSPCFPSELKNYLMFVNIE 409

[Close]

BLAST of Lag0020888 vs. ExPASy TrEMBL
Match: A0A5D3D2G2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003490 PE=4 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 2.5e-202
Identity = 364/418 (87.08%), Postives = 388/418 (92.82%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGED 60
           MDSDFPPLFRCPISMELMEDPVT+STGVTY+RKSIEKWLFTYN KTCPATMQTL A  +D
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60

Query: 61  FVITPNLNLKRLILAWKVTDSSSAPAPAIEEVRSLLATVESSPFKVSSLRKLRSMIEMSD 120
           FVITPNL LKRLILAWK+ DS+S PA A+EEV SLLATVESSPFKVSSLRKLRSMIEM+D
Sbjct: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSMIEMND 120

Query: 121 EMKSEFIRSNGIGIVVNVVAQILVDCSDFSTFEACEEALGVLSQFPISNEHKLFDEMSKR 180
            MKSEF+R +GIGIVVNVV QIL+DCSDFST E CEEALGVL QFPISNE KLFDEM KR
Sbjct: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180

Query: 181 EESMKSISIVLQRGSAEGRFYAVKILRDIAKNTGFNWGSVLEEQGIDFFKPLLELVSDEF 240
           EE MKSI++VLQRGSAEGRFYAVKILR+IAKNT +NWGSV+EEQ IDFFKPLLELVSDEF
Sbjct: 181 EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF 240

Query: 241 PTKASSCALDVMMEILASSKRSRSRAIEAGAVCVLIELLPESGRSKCERMLQILQSLCEC 300
           PTKASSCALDVM+ IL+SSKRSR +AIEAGAV +LIELLPESGRSKCERMLQILQSLCEC
Sbjct: 241 PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSNGATKLGVKILLWISSFHPKERVVEEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVS  ATK+GVKILLWISSFHP+ERVV+EMMACGAVKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360

Query: 361 LLMILHMDSGGGGIGDGGGRSSTKEKVIKILKMHGSSWRRSPCFPSELKNYLKFVNDE 419
           LLMILHMDS   G+GD GGRSSTKEKVIKILKMHG+ WRRSPCFPSELKNYLKFVNDE
Sbjct: 361 LLMILHMDS--VGVGD-GGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

[Close]

BLAST of Lag0020888 vs. ExPASy TrEMBL
Match: A0A6J1E9J6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432079 PE=4 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 4.3e-202
Identity = 364/418 (87.08%), Postives = 387/418 (92.58%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGED 60
           MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYN +TCPATMQT+     D
Sbjct: 13  MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGEND 72

Query: 61  FVITPNLNLKRLILAWKVTDSSSAPAPAIEEVRSLLATVESSPFKVSSLRKLRSMIEMSD 120
           FV+TPNL LKR+ILAWK+ +SSS+PA AI++V+SLLATVESSPFKVSSLRKLRSMIEM D
Sbjct: 73  FVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRD 132

Query: 121 EMKSEFIRSNGIGIVVNVVAQILVDCSDFSTFEACEEALGVLSQFPISNEHKLFDEMSKR 180
           E+KSEFIR NGIGIVVNVV QIL+DCSDFST EACEEALGVLSQFPISNE KLFDEMSKR
Sbjct: 133 EVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDKLFDEMSKR 192

Query: 181 EESMKSISIVLQRGSAEGRFYAVKILRDIAKNTGFNWGSVLEEQGIDFFKPLLELVSDEF 240
           EE MKS++IVLQRGSAEGRFYAVKILR+IAKNT +NWG V+EEQGID FKPLLELVSDEF
Sbjct: 193 EEWMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDLFKPLLELVSDEF 252

Query: 241 PTKASSCALDVMMEILASSKRSRSRAIEAGAVCVLIELLPESGRSKCERMLQILQSLCEC 300
           PTK+SSCALDVM+EILASSKRSR +AIEAGAV +LIELLPES RSKCERMLQILQSLCEC
Sbjct: 253 PTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCERMLQILQSLCEC 312

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSNGATKLGVKILLWISSFHPKERVVEEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVSN ATK+GVKILLWISSFHPKERVVEEMMACGAVKK
Sbjct: 313 AEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILLWISSFHPKERVVEEMMACGAVKK 372

Query: 361 LLMILHMDSGGGGIGDGGGRSSTKEKVIKILKMHGSSWRRSPCFPSELKNYLKFVNDE 419
           LLMILHM SGG G   GGGRSSTKEKVIKILKMHGSSWRR PCFP ELK YLK VND+
Sbjct: 373 LLMILHMASGGVG---GGGRSSTKEKVIKILKMHGSSWRRGPCFPCELKYYLKLVNDD 427

[Close]

BLAST of Lag0020888 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 208.0 bits (528), Expect = 1.5e-53
Identity = 137/420 (32.62%), Postives = 220/420 (52.38%), Query Frame = 0


Query: 4   DFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGEDFVI 63
           + PP F CPIS+E+M+DPV +STG+TY+R SIEKWLF     +CP T Q +     D  +
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDI----TDADL 70

Query: 64  TPNLNLKRLILAWKVTDSS------SAPAPAI--EEVRSLLA-TVESSPFKVSSLRKLRS 123
           TPN  L+RLI +W   ++S        P P I   E+  L+  +  S   +V  L++LR 
Sbjct: 71  TPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQ 130

Query: 124 MIEMSDEMKSEFIRSNGIGIVVNVVAQILVDCSDFSTFEACEEALGVLSQFPISNEHKLF 183
           ++  +   K     +     + N+V+      +D       +EAL +L     S E  L 
Sbjct: 131 IVSENATNKRCLEAAGVPEFLANIVS------NDSENGSLTDEALNLLYHLETS-ETVLK 190

Query: 184 DEMSKREES--MKSISIVLQRGSAEGRFYAVKILRDIAKNTGFNWGSVLEEQGIDFFKPL 243
           + ++ ++++  +KS++ ++QRG  E R YA  +L++I +         L+    + F  +
Sbjct: 191 NLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP---EVFTEV 250

Query: 244 LELVSDEFPTKASSCALDVMMEILASSKRSRSRAIEAGAVCVLIELLPE----SGRSKCE 303
           ++++ D    KA+  A+ +++ I     R+R +A+EAG + V+IELL +    S R   E
Sbjct: 251 VQILDDRISQKATKAAMHILVNICPWG-RNRHKAVEAGVISVIIELLMDESFTSERRGPE 310

Query: 304 RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNGATKLGVKILLWISSFHPKERV 363
             + +L  LC+CAEGR   + HG  IA V KKIL VS  A+   V++LL +  F     +
Sbjct: 311 MAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPAL 370

Query: 364 VEEMMACGAVKKLLMILHMDSGGGGIGDGGGRSSTKEKVIKILKMHGSSWRRSPCFPSEL 409
           + EM+  G V KL ++L +  GG           TKEK  ++LK+H   W+ SPC P  +
Sbjct: 371 LHEMLQLGVVAKLCLVLQVSCGG----------KTKEKAKELLKLHARVWKDSPCLPKNM 405

[Close]

BLAST of Lag0020888 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 193.0 bits (489), Expect = 4.9e-49
Identity = 134/442 (30.32%), Postives = 218/442 (49.32%), Query Frame = 0


Query: 4   DFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGEDFVI 63
           + P  F CPIS+++M+DPV +STG+TY+R+SIEKWLF+    +CP T Q +        +
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD----L 65

Query: 64  TPNLNLKRLILAWKVTDSS------SAPAPAI--EEVRSLLATVESSPF-KVSSLRKLRS 123
           TPN  L+RLI +W   ++S        P P I   E+  L+    SS   +V  L++LR 
Sbjct: 66  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQ 125

Query: 124 MIEMSDEMKSEFIRSNGIGIVVNVVAQIL-------VDCSDFSTFEAC------------ 183
           ++  +   K     +     + N+V+  +          S  +  + C            
Sbjct: 126 IVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSS 185

Query: 184 ----EEALGVLSQFPISNEH-KLFDEMSKREESMKSISIVLQRGSAEGRFYAVKILRDIA 243
               +EAL VL     S    K      K    +K+++ ++QRG  E R YA  +L+ + 
Sbjct: 186 RSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLL 245

Query: 244 KNTGFNWGSVLEEQGIDFFKPLLELVSDEFPTKASSCALDVMMEILASSKRSRSRAIEAG 303
           +        +LE    + F  +++++ D+   KA+  A+ +++ I     R+R +A+E G
Sbjct: 246 EVADPMQIILLER---ELFGEVIQILHDQISHKATRSAMQILV-ITCPWGRNRHKAVEGG 305

Query: 304 AVCVLIELLPE----SGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSN 363
            + ++IELL +    S R   E  + +L  LC+CAEGR   + HG  IA V+KKIL VS 
Sbjct: 306 TISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQ 365

Query: 364 GATKLGVKILLWISSFHPKERVVEEMMACGAVKKLLMILHMDSGGGGIGDGGGRSSTKEK 409
             ++  V++LL +  F     +++EM+  G V KL ++L +  G          + TKEK
Sbjct: 366 ITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCG----------NKTKEK 425

[Close]

BLAST of Lag0020888 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 154.5 bits (389), Expect = 1.9e-37
Identity = 135/449 (30.07%), Postives = 214/449 (47.66%), Query Frame = 0


Query: 4   DFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGEDFVI 63
           + P  F CPIS+E+M+DPVT  +G+TY+R++I KWL      +CP T Q L     D  +
Sbjct: 23  EIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPL---DSDL 82

Query: 64  TPNLNLKRLILAWKVTDSS------SAP------APAIEEVRSLLATVESSPFKVSSLRK 123
           TPN  L+RLI  W V + +      S P         +EE+++L    + +  +  +L+K
Sbjct: 83  TPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQK 142

Query: 124 LRSMIEMSDEMKSEFIRSNGI--GIVVNVVAQILVDCSDFSTFEACEEALGVLSQFPI-S 183
           L   +   D      +   G+   +++ VV     D       +  +E+L +L    I S
Sbjct: 143 LE--VLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPS 202

Query: 184 NEHKLFDEMSKREESMKSISIVLQRGSAEGRFYAVKILRDIAKNTGFNWGSVLEEQ---- 243
           N+ K    + + +  M+S++ VL +     + Y + +LR++ + T  +    L  +    
Sbjct: 203 NDAKTI--LMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIFKG 262

Query: 244 GIDFFKPLLELVSDEFPT----------------------------KASSCALDVMMEIL 303
            I F K ++  V+   PT                            +A + AL +++E  
Sbjct: 263 IIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLE-T 322

Query: 304 ASSKRSRSRAIEAGAVCVLIEL-LPESGRSK-CERMLQILQSLCECAEGRLALVEHGMGI 363
           +S  R+RS  ++ GAV  LIEL +  +G  +  E ML +L  LC CA GR  ++ H  GI
Sbjct: 323 SSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGI 382

Query: 364 AAVTKKILNVSNGATKLGVKILLWISSFHPKERVVEEMMACGAVKKLLMILHMDSGGGGI 404
           A VTK++L VS  A    + IL  +S F P+  VVEEM+  G V+KL  +L MD G    
Sbjct: 383 AVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCG---- 442

[Close]

BLAST of Lag0020888 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 144.8 bits (364), Expect = 1.5e-34
Identity = 121/411 (29.44%), Postives = 194/411 (47.20%), Query Frame = 0


Query: 6   PPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGEDFVITP 65
           P  FRCPIS+ELM+DPVT+ TG TY+R SIE W+   NN TCP T   L     DF + P
Sbjct: 15  PYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPL----SDFTLIP 74

Query: 66  NLNLKRLILAWKVTDSSSA----PAP-----------AIEEVRSLLATVESSPFKVSSLR 125
           N  L+RLI  W V + S+     P P            + +  ++  T  S   + ++LR
Sbjct: 75  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALR 134

Query: 126 KLRSMIEMSDEMKSEFIRSNGIGIVVNVVAQILVDCSDFSTFEACEEALGVLSQFPISNE 185
           +LR     SD+ +      N   I++ ++       S+ ++ E   E+L +L   PI+ E
Sbjct: 135 RLRGFARDSDKNRVLIAAHNATEILIKIL------FSETTSSELVSESLALLVMLPIT-E 194

Query: 186 HKLFDEMSKREESMKSISIVLQRGSAEGRFYAVKILRDIAKNT--GFNWGSVLEEQGIDF 245
              F  +S     ++ ++ +L   S E R  A  ++  ++  T      GS+   + +  
Sbjct: 195 PNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESV-- 254

Query: 246 FKPLLELVSDEFPTKASSCALDVMMEILASSKRSRSRAIEAGAVCVLIE-LLPESGRSKC 305
           F+ +L+L+ +   ++ +       +  L S K +R  AI AGA  +LI+ L  +  R   
Sbjct: 255 FEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDT 314

Query: 306 ERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNGATKLGVKILLWISSFHPKER 365
           ER L  ++ LC   EG  A  EH + +  + K IL VS+ AT+     LL + +   +ER
Sbjct: 315 ERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCT--AEER 374

Query: 366 VVEEMMACGAVKKLLMILHMDSGGGGIGDGGGRSSTKEKVIKILKMHGSSW 399
             EE    G V +LL+++  +               K+K  K+LK+   SW
Sbjct: 375 WREEAAGAGVVVQLLLMVQSEC----------TERAKKKAQKLLKLLRDSW 400

[Close]

BLAST of Lag0020888 vs. TAIR 10
Match: AT5G37490.1 (ARM repeat superfamily protein )

HSP 1 Score: 131.0 bits (328), Expect = 2.3e-30
Identity = 121/424 (28.54%), Postives = 197/424 (46.46%), Query Frame = 0


Query: 6   PPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNNKTCPATMQTLDAAGEDFVITP 65
           PP F+CPIS++LM+DPV ISTG+TY+R SIE W+    NKTCP T   L      F   P
Sbjct: 32  PPEFQCPISIDLMKDPVIISTGITYDRVSIETWI-NSGNKTCPVTNTVLTT----FDQIP 91

Query: 66  NLNLKRLILAWKVTDSSSAPAPAIEEVRSLLATVESSPFKVSSL-RKLRSMIEMSDEMKS 125
           N  ++++I  W V   S    P I+ + +    V   P +V  + RKL S     D  K 
Sbjct: 92  NHTIRKMIQGWCVEKGS----PLIQRIPT--PRVPLMPCEVYEISRKLSSATRRGDYEKC 151

Query: 126 EFI-----------RSNGIGIVVNVVAQILVDCSD-FSTFE----ACEEALGVLS-QFPI 185
             I             N   +  N V  +L DC D FS  E       E L +L+  FPI
Sbjct: 152 GVIIEKIKKLGDESEKNRKCVNENSVGWVLCDCFDKFSGDEKLTFMLNEILSLLTWMFPI 211

Query: 186 SNEHKLFDEMSK--REESMKSISIVLQRGSAEGRFYAVKILRDIAKNTGFNWGSVLEEQG 245
                  + +SK     S + ++ +L+      R  A  I+++I         S   E G
Sbjct: 212 G-----LEGISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENG 271

Query: 246 IDFFKPLLELVSDEFPTKASSCALDVMMEILASSKRSRSRAIEAGAVCVLIELLPESGRS 305
           +   + L++L+ D   + ++  +L  + +++       S  +E G V + +E++ ++  S
Sbjct: 272 V--AEALVKLIRDSVSSSSTKSSLIAIYQMVLQKPEIASEFLEIGLVSITVEMIVDAENS 331

Query: 306 KCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNGATKLGVKIL--LWISSFH 365
            CE+ L +L ++CE   GR  + ++ + +  + KKI  VS  AT+  + ++  LW +   
Sbjct: 332 VCEKALAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTG-- 391

Query: 366 PKERVVEEMMACGAVKKLLMILHMDSGGGGIGDGGGRSSTKEKVIKILKMHGSSWR-RSP 407
                VE+ +  GA +K+L++L +  G            TKEK  ++LKM  +  +  S 
Sbjct: 392 -NTVAVEDAVRLGAFQKVLLVLQVGYG----------EETKEKATELLKMMNTQMKLMSD 424

The following BLAST results are available for this feature:

BLAST of Lag0020888 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902365.1	6.4e-208	89.71	E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida]	[more]
KAG7028702.1	7.8e-206	90.67	E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022959873.1	2.3e-205	90.43	E3 ubiquitin-protein ligase PUB23-like isoform X2 [Cucurbita moschata]	[more]
KAG6597231.1	3.0e-205	90.43	E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_004134357.1	3.6e-203	87.08	E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN56649.1 hypothetical pro...	[more]

BLAST of Lag0020888 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84TG3	2.1e-52	32.62	E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ...	[more]
Q9SVC6	6.9e-48	30.32	E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ...	[more]
Q9SF15	2.7e-36	30.07	E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ...	[more]
Q9LT79	2.2e-33	29.44	U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2...	[more]
Q5PNY6	3.2e-29	28.54	U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2...	[more]

BLAST of Lag0020888 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H765	1.1e-205	90.43	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460728...	[more]
A0A0A0L6J2	1.7e-203	87.08	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G127120 PE...	[more]
A0A6J1IKT9	3.9e-203	89.71	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111474953 P...	[more]
A0A5D3D2G2	2.5e-202	87.08	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6J1E9J6	4.3e-202	87.08	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432079...	[more]

BLAST of Lag0020888 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G35930.1	1.5e-53	32.62	plant U-box 23	[more]
AT3G52450.1	4.9e-49	30.32	plant U-box 22	[more]
AT3G11840.1	1.9e-37	30.07	plant U-box 24	[more]
AT3G19380.1	1.5e-34	29.44	plant U-box 25	[more]
AT5G37490.1	2.3e-30	28.54	ARM repeat superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 8..76 e-value: 2.1E-20 score: 83.8
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 6..78 e-value: 2.1E-14 score: 53.5
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 4..83 score: 21.585274
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 84..393 e-value: 1.6E-13 score: 52.2
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 1..83 e-value: 2.6E-22 score: 80.6
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 5..416
None	No IPR available	PANTHER	PTHR22849:SF23	RING-TYPE E3 UBIQUITIN TRANSFERASE	coord: 5..416
None	No IPR available	CDD	cd16664	RING-Ubox_PUB	coord: 9..52 e-value: 8.48773E-22 score: 85.7482
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 3..79
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 88..371

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0020888.1	Lag0020888.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity