Lag0020868 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0020868
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionB3 domain-containing protein
Locationchr7: 2715324 .. 2716456 (-)
RNA-Seq ExpressionLag0020868
SyntenyLag0020868
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGTCAGAAGAGAGACTTTCGACAGAGTTTGAAACAACGGAGCCGCAAAATGATGAAATTTTGCCCCTCACAGGAAAACCCTACCATGGCGTTATTCTTTCTAAATCGCATGTCGCTCCCATTTGTAATATGGTGAGTTCTCAGTCTCATGGAAATTCACTCAGAAATTTGTGAACAGTTAGTATTATAAACTAAATTGGGTTCTGGGTGCTTGCATTGCAAATACCACTAAATTTTATGGCCAATCCTGGAAGTAGCTGGCTAGTTTGATCTACAATAGCTTTTGTATTCTTTTGTTATTATTGTCTCCTTCAGAAATATTTGAAGAGAAACTTGCAAGATTAACTAGTCTGAGACTCTGAAACTATTATAAAAACGGTCTAGAACAAATGTTTCGTGGGCTCAGAATTTCAGATAAATACTTTACAAGTTGGTCTATTGCTATTTCTTGTAGTATTTGATATTTGACGTCTTTTAATACACACACACACACACATATATATATATATATACACATACACATATACACATATATATGTACATGTATATGTATGCATGTATGTTTGTTGAAAGATTGTTATTAATTCCAAAATCACGAGCATGAAACATCAACTTTTAGAGGTCATTCAATCACAAATTTAAACAAATGGTTTTGAAACAAAGAAAATTTTCTTGAAAACAGCCATCAATCTCCTGTTCAGAATCACGATCATTAGGCAGTACCAAATTCTTAGCTAATGCCAAATGCTCAAATTAGGCATTTTTTAGGCATATAAATAAACCAGCTTAATTGTGAAGGACTATCATATCTTCATTTGCAGGTTTTGCCAGCCAAATTTCATTCTGTTTTACCTGCTATCATAATCCCTGCGGTTCTTTACTGTGGGGACAAGAAATGGAAGATAGATTATTATGGGAACCGACGTGGTAAATCACTTGACACAAGACAGTGGAGGAAGTTTGTTAATGATAATCATCTCAAGTCTGGAGATGCTTGTGTATTTGAGCTAATAGAATGCAGCAGCAGCTTACTCAAATTCAGAGTTCAAATTCTCAGAGGTGACATCCCCGTCCCGCTGCACGACAAGTTCAGTGGGGAGACTAAAGACACCCCTATATTCATTGACTAG

mRNA sequence

ATGCAGTCAGAAGAGAGACTTTCGACAGAGTTTGAAACAACGGAGCCGCAAAATGATGAAATTTTGCCCCTCACAGGAAAACCCTACCATGGCGTTATTCTTTCTAAATCGCATGTCGCTCCCATTTGTAATATGGTTTTGCCAGCCAAATTTCATTCTGTTTTACCTGCTATCATAATCCCTGCGGTTCTTTACTGTGGGGACAAGAAATGGAAGATAGATTATTATGGGAACCGACGTGGTAAATCACTTGACACAAGACAGTGGAGGAAGTTTGTTAATGATAATCATCTCAAGTCTGGAGATGCTTGTGTATTTGAGCTAATAGAATGCAGCAGCAGCTTACTCAAATTCAGAGTTCAAATTCTCAGAGGTGACATCCCCGTCCCGCTGCACGACAAGTTCAGTGGGGAGACTAAAGACACCCCTATATTCATTGACTAG

Coding sequence (CDS)

ATGCAGTCAGAAGAGAGACTTTCGACAGAGTTTGAAACAACGGAGCCGCAAAATGATGAAATTTTGCCCCTCACAGGAAAACCCTACCATGGCGTTATTCTTTCTAAATCGCATGTCGCTCCCATTTGTAATATGGTTTTGCCAGCCAAATTTCATTCTGTTTTACCTGCTATCATAATCCCTGCGGTTCTTTACTGTGGGGACAAGAAATGGAAGATAGATTATTATGGGAACCGACGTGGTAAATCACTTGACACAAGACAGTGGAGGAAGTTTGTTAATGATAATCATCTCAAGTCTGGAGATGCTTGTGTATTTGAGCTAATAGAATGCAGCAGCAGCTTACTCAAATTCAGAGTTCAAATTCTCAGAGGTGACATCCCCGTCCCGCTGCACGACAAGTTCAGTGGGGAGACTAAAGACACCCCTATATTCATTGACTAG

Protein sequence

MQSEERLSTEFETTEPQNDEILPLTGKPYHGVILSKSHVAPICNMVLPAKFHSVLPAIIIPAVLYCGDKKWKIDYYGNRRGKSLDTRQWRKFVNDNHLKSGDACVFELIECSSSLLKFRVQILRGDIPVPLHDKFSGETKDTPIFID
Homology
[Close]
BLAST of Lag0020868 vs. NCBI nr
Match: XP_022974195.1 (B3 domain-containing protein Os04g0386900-like [Cucurbita maxima] >XP_022974196.1 B3 domain-containing protein Os04g0386900-like [Cucurbita maxima] >XP_022974197.1 B3 domain-containing protein Os04g0386900-like [Cucurbita maxima] >XP_022975683.1 B3 domain-containing protein Os04g0386900-like [Cucurbita maxima] >XP_022975684.1 B3 domain-containing protein Os04g0386900-like [Cucurbita maxima] >XP_022975685.1 B3 domain-containing protein Os04g0386900-like [Cucurbita maxima])

HSP 1 Score: 256.1 bits (653), Expect = 1.8e-64
Identity = 120/147 (81.63%), Postives = 132/147 (89.80%), Query Frame = 0 

Query: 1   MQSEERLSTEFETTEPQNDEILPLTGKPYHGVILSKSHVAPICNMVLPAKFHSVLPAIII 60
           MQS+E+  TE    +PQN  ILPL+GKPYHGVILSKSHVAPICNM LPAKFHSVLPAI+I
Sbjct: 1   MQSQEKPPTEPTEMQPQNSRILPLSGKPYHGVILSKSHVAPICNMFLPAKFHSVLPAIMI 60

Query: 61  PAVLYCGDKKWKIDYYGNRRGKSLDTRQWRKFVNDNHLKSGDACVFELIECSSSLLKFRV 120
           PAVLYC  KKWKIDYYGNRRGK+LDT+QWRKFVNDNHLK GDACVFELIECSSS+LKFRV
Sbjct: 61  PAVLYCEGKKWKIDYYGNRRGKALDTKQWRKFVNDNHLKCGDACVFELIECSSSVLKFRV 120

Query: 121 QILRGDIPVPLHDKFSGETKDTPIFID 148
           QILRGDIP  LH+KFSGE+++TPI ID
Sbjct: 121 QILRGDIPFELHEKFSGESRNTPILID 147

[Close]
BLAST of Lag0020868 vs. NCBI nr
Match: XP_023539026.1 (B3 domain-containing protein Os04g0386900-like [Cucurbita pepo subsp. pepo] >KAG6597214.1 B3 domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028685.1 B3 domain-containing protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 254.6 bits (649), Expect = 5.2e-64
Identity = 118/147 (80.27%), Postives = 132/147 (89.80%), Query Frame = 0 

Query: 1   MQSEERLSTEFETTEPQNDEILPLTGKPYHGVILSKSHVAPICNMVLPAKFHSVLPAIII 60
           MQS+E+  TE    +PQN  ILPL+GKPYHGVILSKSHVAPICNM +PAKFHSVLPAI+I
Sbjct: 1   MQSQEKPPTEPTEMQPQNSRILPLSGKPYHGVILSKSHVAPICNMFMPAKFHSVLPAIMI 60

Query: 61  PAVLYCGDKKWKIDYYGNRRGKSLDTRQWRKFVNDNHLKSGDACVFELIECSSSLLKFRV 120
           PAVLYC  KKWKIDYYGNRRGK+LDT+QWRKFVNDNHLK GDACVFELIECSSS++KFRV
Sbjct: 61  PAVLYCEGKKWKIDYYGNRRGKALDTKQWRKFVNDNHLKCGDACVFELIECSSSVIKFRV 120

Query: 121 QILRGDIPVPLHDKFSGETKDTPIFID 148
           QILRGDIP  LH+KFSGE+++TPI ID
Sbjct: 121 QILRGDIPFELHEKFSGESRNTPILID 147

[Close]
BLAST of Lag0020868 vs. NCBI nr
Match: XP_022941967.1 (B3 domain-containing protein Os04g0386900-like [Cucurbita moschata] >XP_022941973.1 B3 domain-containing protein Os04g0386900-like [Cucurbita moschata])

HSP 1 Score: 253.1 bits (645), Expect = 1.5e-63
Identity = 118/147 (80.27%), Postives = 131/147 (89.12%), Query Frame = 0 

Query: 1   MQSEERLSTEFETTEPQNDEILPLTGKPYHGVILSKSHVAPICNMVLPAKFHSVLPAIII 60
           MQS+E+   E    +PQN  ILPL+GKPYHGVILSKSHVAPICNM LPAKFHSVLPAI+I
Sbjct: 1   MQSQEKPPPEPTEMQPQNSRILPLSGKPYHGVILSKSHVAPICNMFLPAKFHSVLPAIMI 60

Query: 61  PAVLYCGDKKWKIDYYGNRRGKSLDTRQWRKFVNDNHLKSGDACVFELIECSSSLLKFRV 120
           PAVLYC  KKWKIDYYGNRRGK+LDT+QWRKFVNDNHLK GDACVFELIECSSS++KFRV
Sbjct: 61  PAVLYCEGKKWKIDYYGNRRGKALDTKQWRKFVNDNHLKCGDACVFELIECSSSVIKFRV 120

Query: 121 QILRGDIPVPLHDKFSGETKDTPIFID 148
           QILRGDIP  LH+KFSGE+++TPI ID
Sbjct: 121 QILRGDIPFELHEKFSGESRNTPILID 147

[Close]
BLAST of Lag0020868 vs. NCBI nr
Match: XP_038903804.1 (B3 domain-containing protein Os04g0386900-like [Benincasa hispida] >XP_038903811.1 B3 domain-containing protein Os04g0386900-like [Benincasa hispida])

HSP 1 Score: 253.1 bits (645), Expect = 1.5e-63
Identity = 116/147 (78.91%), Postives = 132/147 (89.80%), Query Frame = 0 

Query: 1   MQSEERLSTEFETTEPQNDEILPLTGKPYHGVILSKSHVAPICNMVLPAKFHSVLPAIII 60
           MQS+ +  TE  T EPQ+ EILPL+GKPYH  ILSKSHVAPIC++VLPAKFHS+LPAI+I
Sbjct: 1   MQSQRKPPTELPTMEPQDGEILPLSGKPYHAFILSKSHVAPICSLVLPAKFHSILPAIVI 60

Query: 61  PAVLYCGDKKWKIDYYGNRRGKSLDTRQWRKFVNDNHLKSGDACVFELIECSSSLLKFRV 120
           PAVL+C  KKW IDYYGNRRGK+LDT+QWRKFVNDN LKSGDACVFEL+ECSSS+LKFRV
Sbjct: 61  PAVLFCQGKKWTIDYYGNRRGKALDTKQWRKFVNDNRLKSGDACVFELMECSSSILKFRV 120

Query: 121 QILRGDIPVPLHDKFSGETKDTPIFID 148
           QILRGDIP  LHDKFSGETKDTPI+++
Sbjct: 121 QILRGDIPFQLHDKFSGETKDTPIYLE 147

[Close]
BLAST of Lag0020868 vs. NCBI nr
Match: XP_022147119.1 (B3 domain-containing protein Os04g0386900-like [Momordica charantia])

HSP 1 Score: 248.1 bits (632), Expect = 4.9e-62
Identity = 117/147 (79.59%), Postives = 129/147 (87.76%), Query Frame = 0 

Query: 1   MQSEERLSTEFETTEPQNDEILPLTGKPYHGVILSKSHVAPICNMVLPAKFHSVLPAIII 60
           MQS+E+  +E  T EP+N EI PL+ KP+HGVIL+KSHVAPI NM LPAKFHSVLPAI+I
Sbjct: 1   MQSQEKPPSEHTTMEPRNYEIFPLSDKPFHGVILAKSHVAPIYNMTLPAKFHSVLPAIVI 60

Query: 61  PAVLYCGDKKWKIDYYGNRRGKSLDTRQWRKFVNDNHLKSGDACVFELIECSSSLLKFRV 120
             VLYCG K+W +DYYGNRRGKSLDTR WRKFVNDNHLKSGDACVFEL+ECSSS LKFRV
Sbjct: 61  HGVLYCGGKEWTVDYYGNRRGKSLDTRHWRKFVNDNHLKSGDACVFELMECSSSTLKFRV 120

Query: 121 QILRGDIPVPLHDKFSGETKDTPIFID 148
           QILRGDIPV LHDKFSGE+KDTPI ID
Sbjct: 121 QILRGDIPVQLHDKFSGESKDTPIKID 147

[Close]
BLAST of Lag0020868 vs. ExPASy Swiss-Prot
Match: Q7XLP4 (B3 domain-containing protein Os04g0386900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0386900 PE=2 SV=3)

HSP 1 Score: 98.6 bits (244), Expect = 6.3e-20
Identity = 53/130 (40.77%), Postives = 72/130 (55.38%), Query Frame = 0 

Query: 21  ILPLTGKPYHGVILSKSHVAPICNMVLPAKFHSVLPAIIIPAVLYCGDKKWKIDYYGNRR 80
           +LPL GKPY   I+ KSHV P   +V+P  F  +LP+   PA L    + W + + G R 
Sbjct: 84  VLPLLGKPYFTCIMCKSHVQPPFQVVVPRSFAPLLPSRTTPATLSWRGRSWGMRFTGGRL 143

Query: 81  GKSLDTRQWRKFVNDNHLKSGDACVFELI---ECSSSLLKFRVQILRGDIPVPLHDKFSG 140
            + L+   WR F  DN L+ GD CVFEL+         ++FRVQ+LR +IP  +  +  G
Sbjct: 144 IQRLEA-GWRGFAVDNDLRLGDGCVFELLVGGGGEQERVEFRVQVLRAEIPARIRGRAGG 203

Query: 141 ETKDTPIFID 148
            T  TPI ID
Sbjct: 204 YTSATPIVID 212

[Close]
BLAST of Lag0020868 vs. ExPASy Swiss-Prot
Match: Q9LHY9 (B3 domain-containing protein Os06g0112300 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0112300 PE=2 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 7.2e-16
Identity = 50/124 (40.32%), Postives = 68/124 (54.84%), Query Frame = 0 

Query: 26  GKPYHGVILSKSHVAPICNMVLPAKFHSVL--PAIIIPAVLYCGDKKWKIDYYGNRRGKS 85
           G P+   +++KSH+ P   M +P +F   L  P     AVL+ G K W   Y G+ + K 
Sbjct: 52  GNPFFTALIAKSHLHPKFQMWIPPRFQHRLAEPEARTAAVLHSGGKSWATSYCGHLKMKK 111

Query: 86  LDTRQWRKFVNDNHLKSGDACVFELIEC-SSSLLKFRVQILRGDIPVPLHDKFSGETKDT 145
           LD   W +F  DN L  GDACVFEL+   ++  L+F+VQILRG +P  +     G T D 
Sbjct: 112 LDA-GWSEFAVDNRLLVGDACVFELVAMGAAGGLEFQVQILRGGLPAEVVTS-KGLTSDQ 171

Query: 146 PIFI 147
           PI I
Sbjct: 172 PILI 173

[Close]
BLAST of Lag0020868 vs. ExPASy Swiss-Prot
Match: Q8RYD1 (B3 domain-containing protein REM16 OS=Arabidopsis thaliana OX=3702 GN=REM16 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 6.1e-07
Identity = 34/99 (34.34%), Postives = 49/99 (49.49%), Query Frame = 0 

Query: 32  VILSKSHVAPICNMVLPAKFHSVLPAIII--PAVLYCGDKKW--KIDYYGNRRGKSLDTR 91
           V + +SHV   C + +P K+  V   +I     V+     KW  K + +G R    + T 
Sbjct: 225 VFMKRSHVVSKCFLTIPYKW-CVKNMLITRQEVVMQVDQTKWEMKFNIFGARGSGGIST- 284

Query: 92  QWRKFVNDNHLKSGDACVFELIECSSSLLKFRVQILRGD 127
            W+KFV DN+L+ GD CVFE     +  L   V I RG+
Sbjct: 285 GWKKFVQDNNLREGDVCVFEPANSETKPLHLNVYIFRGE 321

[Close]
BLAST of Lag0020868 vs. ExPASy Swiss-Prot
Match: Q9XIB5 (B3 domain-containing protein REM19 OS=Arabidopsis thaliana OX=3702 GN=REM19 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 7.4e-05
Identity = 37/123 (30.08%), Postives = 58/123 (47.15%), Query Frame = 0 

Query: 2   QSEERLSTEFETTEPQNDEILPLTGKPYHGVILSKSHVAPICNMVLPAKF-HSVLPAIII 61
           +  ER     +T EP N         PY  V+L  S++   C M LP+ F    L  I  
Sbjct: 110 EERERAVNAAKTFEPTN---------PYFRVVLRPSYLYRGCIMYLPSGFAEKYLSGISG 169

Query: 62  PAVLYCGDKKWKIDYYGNRRGKSLDTRQWRKFVNDNHLKSGDACVFELIECSSSLLK--- 121
              L  G+K+W +     + G++  ++ W +F  +N++  GD CVFEL+     +L+   
Sbjct: 170 FIKLQLGEKQWPVRCL-YKAGRAKFSQGWYEFTLENNIGEGDVCVFELLRTRDFVLEVTA 222

[Close]
BLAST of Lag0020868 vs. ExPASy Swiss-Prot
Match: Q2QMT5 (B3 domain-containing protein LOC_Os12g40090 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0591500 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 9.7e-05
Identity = 29/105 (27.62%), Postives = 51/105 (48.57%), Query Frame = 0 

Query: 14  TEPQNDEILPLT----GKPYHGVILSKSHVAPICNMVLPAKF---HSVLPAIIIPAVLYC 73
           T  + DEI  L     G P   V+L  +HV     +++P +F   H    +  I  +   
Sbjct: 207 TGEERDEIFSLVSLQPGNPVFVVVLQTAHVRRRNILIVPTRFAADHLERKSHDILLIRPN 266

Query: 74  GDKKWKIDYYG-NRRGKSLDTRQWRKFVNDNHLKSGDACVFELIE 111
             +KW + YY  +   +  +  +W KF+ +N L+ G+ C+FEL++
Sbjct: 267 RKQKWSVKYYYLSNTTRGFNCHRWIKFIRENRLREGNVCIFELMK 311

The following BLAST results are available for this feature:
BLAST of Lag0020868 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022974195.11.8e-6481.63B3 domain-containing protein Os04g0386900-like [Cucurbita maxima] >XP_022974196.... [more]
XP_023539026.15.2e-6480.27B3 domain-containing protein Os04g0386900-like [Cucurbita pepo subsp. pepo] >KAG... [more]
XP_022941967.11.5e-6380.27B3 domain-containing protein Os04g0386900-like [Cucurbita moschata] >XP_02294197... [more]
XP_038903804.11.5e-6378.91B3 domain-containing protein Os04g0386900-like [Benincasa hispida] >XP_038903811... [more]
XP_022147119.14.9e-6279.59B3 domain-containing protein Os04g0386900-like [Momordica charantia][more]
BLAST of Lag0020868 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q7XLP46.3e-2040.77B3 domain-containing protein Os04g0386900 OS=Oryza sativa subsp. japonica OX=399... [more]
Q9LHY97.2e-1640.32B3 domain-containing protein Os06g0112300 OS=Oryza sativa subsp. japonica OX=399... [more]
Q8RYD16.1e-0734.34B3 domain-containing protein REM16 OS=Arabidopsis thaliana OX=3702 GN=REM16 PE=2... [more]
Q9XIB57.4e-0530.08B3 domain-containing protein REM19 OS=Arabidopsis thaliana OX=3702 GN=REM19 PE=1... [more]
Q2QMT59.7e-0527.62B3 domain-containing protein LOC_Os12g40090 OS=Oryza sativa subsp. japonica OX=3... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003340B3 DNA binding domainSMARTSM01019B3_2coord: 34..126
e-value: 1.2E-4
score: 31.4
IPR003340B3 DNA binding domainPFAMPF02362B3coord: 33..122
e-value: 7.8E-12
score: 44.9
IPR003340B3 DNA binding domainPROSITEPS50863B3coord: 69..126
score: 10.489346
IPR003340B3 DNA binding domainCDDcd10017B3_DNAcoord: 33..124
e-value: 1.01321E-16
score: 68.8937
IPR015300DNA-binding pseudobarrel domain superfamilyGENE3D2.40.330.10coord: 6..126
e-value: 2.4E-18
score: 68.1
IPR015300DNA-binding pseudobarrel domain superfamilySUPERFAMILY101936DNA-binding pseudobarrel domaincoord: 27..124
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
NoneNo IPR availablePANTHERPTHR31391:SF64B3 DOMAIN-CONTAINING PROTEIN OS04G0386900coord: 4..140
IPR044837B3 domain-containing protein REM16-likePANTHERPTHR31391B3 DOMAIN-CONTAINING PROTEIN OS11G0197600-RELATEDcoord: 4..140

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0020868.1Lag0020868.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding