Homology
BLAST of Lag0020772 vs. NCBI nr
Match:
XP_038878214.1 (K(+) efflux antiporter 2, chloroplastic [Benincasa hispida])
HSP 1 Score: 2070.0 bits (5362), Expect = 0.0e+00
Identity = 1130/1209 (93.47%), Postives = 1158/1209 (95.78%), Query Frame = 0
Query: 252 WKQNVLCGSDRTYCKTLDQFGPGNVLRNRIIDAKLTGGSRVFYKVPKKRNRIVASINSNH 311
WKQNVLCGS+RT CKT DQFGP +VLRNRIIDAKLTGGSRV YKVPKKRNRIVAS NSNH
Sbjct: 8 WKQNVLCGSERTSCKTFDQFGPSSVLRNRIIDAKLTGGSRVSYKVPKKRNRIVASSNSNH 67
Query: 312 LSLVCASKFDGALQIFNHKRFLNFNNKIIGRRTRMVNSECQSNDSLAFIDGNGRNVEYVD 371
LSLVCASKFDGALQIF HK+FLN+NNKI GRR MV ECQ+NDSLAFIDGNGRN+E V+
Sbjct: 68 LSLVCASKFDGALQIFRHKKFLNWNNKISGRRMGMVQLECQNNDSLAFIDGNGRNMECVN 127
Query: 372 TSDEGSSSEPTDGVGSAGSKEAGGEAEAVETDAPTVDELRELLQKAMTELEVARLNSTMF 431
T DEGSSSEPTDGVGSAGS+E GGEAE VE D PTVDELRELLQKAM ELEVARLNSTMF
Sbjct: 128 TGDEGSSSEPTDGVGSAGSREVGGEAETVEADVPTVDELRELLQKAMKELEVARLNSTMF 187
Query: 432 EEKAQKISETAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMALSLAEARL 491
EEKAQKISE AIALQDEA A NDVNSTLDSVQQIVNEEYAAKEAVQKATMALSLAEARL
Sbjct: 188 EEKAQKISEAAIALQDEATIARNDVNSTLDSVQQIVNEEYAAKEAVQKATMALSLAEARL 247
Query: 492 QVATESLDLAKGGNDSPETSIDSEGEIDGKEEQEVLLVAQEDIKECRANLEICDAELKRV 551
QVA ESL+LAKGGND PETSIDSEG IDGKEE+E+LLVAQEDIKECRANLE CDAELKR+
Sbjct: 248 QVAIESLELAKGGNDFPETSIDSEGTIDGKEEREMLLVAQEDIKECRANLENCDAELKRL 307
Query: 552 QSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVSDAEIVL 611
QSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIM LAEQAVAFELEAAQRVSDAEI L
Sbjct: 308 QSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMQLAEQAVAFELEAAQRVSDAEIAL 367
Query: 612 QKVEKSLSNSFVDTSETTQGSNVIEEVVNEDNKAVLEISGDIAVEMDRDLPLSGDSLAVK 671
QKVEKSLS+SFVDT E TQGSNVIEEV EDNKAVLEISGDIAVEMDRDLPLSGDSL++K
Sbjct: 368 QKVEKSLSSSFVDTLEITQGSNVIEEVETEDNKAVLEISGDIAVEMDRDLPLSGDSLSIK 427
Query: 672 SLPGSFSDSEDSDQAYDLSDNDNGKLSSDSFKEVEAGAEKSVLSQAKKQETQKDLTREGS 731
SLPGSFSDSEDSDQ Y LSD++NGKLSSD KEVE+GAEKS+LSQ KKQETQKDLTREGS
Sbjct: 428 SLPGSFSDSEDSDQPYYLSDSENGKLSSDFAKEVESGAEKSILSQTKKQETQKDLTREGS 487
Query: 732 PLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALV----LDFTKKQLPKLVVGVVLLGAG 791
PLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALV LD TKKQLPKL+VG VLLGAG
Sbjct: 488 PLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALVFQGLLDSTKKQLPKLIVGAVLLGAG 547
Query: 792 ITIFSNRAERSSQMFQQPDAVSISTDEVSLNTKPLFQQLRKLPKRVKKLIAKIPHQEVNE 851
I IF+NRAERSSQM QQPD V+ STDEVSLNTKPL QQLRKLPKRVKKLI+++PHQEVNE
Sbjct: 548 IAIFANRAERSSQMIQQPDVVTTSTDEVSLNTKPLLQQLRKLPKRVKKLISQLPHQEVNE 607
Query: 852 EEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAE 911
EEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAE
Sbjct: 608 EEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAE 667
Query: 912 FGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPGPAAIVIG 971
FGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQ GPAAIVIG
Sbjct: 668 FGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQAGPAAIVIG 727
Query: 972 NGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSKGGIG 1031
NGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSKGGIG
Sbjct: 728 NGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSKGGIG 787
Query: 1032 FQAIAEALGVAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILGTSLL 1091
FQAIAEALG+AAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILGTSLL
Sbjct: 788 FQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILGTSLL 847
Query: 1092 TARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNF 1151
TARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNF
Sbjct: 848 TARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNF 907
Query: 1152 PVITGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEAVNQGIMS 1211
PVI GSLGLLIGGKTILVALVGR FGISIISA+RVGLLLAPGGEFAFVAFGEAVNQGIMS
Sbjct: 908 PVIMGSLGLLIGGKTILVALVGRFFGISIISAIRVGLLLAPGGEFAFVAFGEAVNQGIMS 967
Query: 1212 SQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIIICGFG 1271
SQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIIICGFG
Sbjct: 968 SQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIIICGFG 1027
Query: 1272 RVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERACAAA 1331
RVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERACAAA
Sbjct: 1028 RVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERACAAA 1087
Query: 1332 ITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAA 1391
ITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAA
Sbjct: 1088 ITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAA 1147
Query: 1392 VLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTSDSSDEN 1451
VLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTSDSSDEN
Sbjct: 1148 VLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTSDSSDEN 1207
Query: 1452 QITEGTLAI 1457
Q+TEGTLAI
Sbjct: 1208 QVTEGTLAI 1216
BLAST of Lag0020772 vs. NCBI nr
Match:
KAG7028620.1 (K(+) efflux antiporter 2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 2051.2 bits (5313), Expect = 0.0e+00
Identity = 1136/1282 (88.61%), Postives = 1182/1282 (92.20%), Query Frame = 0
Query: 178 TVIAGASFFPTNFSAADVSPLFSQPSASSCAPCEQLYVLLIDSFELNARVTDRMDGTMCL 237
TVIAGA F P SAAD+SPLF QP AS + + GTM L
Sbjct: 12 TVIAGALFSP-RLSAADISPLFYQPPASGLS---------------------HIPGTMDL 71
Query: 238 IECQQWLNDWQSC-IWKQNVLCGSDRTYCKTLDQFGPGNVLRNRIIDAKLTGGSRVFYKV 297
SC IWKQNVL GS++ YCKTLDQFGP VLR+RIIDAKLTGGSRV YKV
Sbjct: 72 -----------SCNIWKQNVLSGSEKAYCKTLDQFGPSYVLRSRIIDAKLTGGSRVIYKV 131
Query: 298 PKKRNRIVASINSNHLSLVCASKFDGALQIFNHKRFLNFNNKIIGRRTRMVNSECQSNDS 357
PKKRNRIVA+ NSNHLSLVCASK+DGALQI HKRFLN NN GRR MV SECQSNDS
Sbjct: 132 PKKRNRIVAASNSNHLSLVCASKYDGALQICKHKRFLNCNN-TFGRRMGMVYSECQSNDS 191
Query: 358 LAFIDGNGRNVEYVDTSDEGSSSEPTDGVGSAGSKEAGGEAEAVETDAPTVDELRELLQK 417
+FIDGNGRN+EYV T D GSSSE TDG+GSAGS+EAGGEAEAVETDA TVDELRELLQK
Sbjct: 192 SSFIDGNGRNLEYVGTGDGGSSSEHTDGLGSAGSREAGGEAEAVETDALTVDELRELLQK 251
Query: 418 AMTELEVARLNSTMFEEKAQKISETAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEA 477
AMTELEVARLNSTMFEEKAQKISE AIALQDEAAHAWNDVNS LDSVQQ+VNEEYAAKEA
Sbjct: 252 AMTELEVARLNSTMFEEKAQKISEAAIALQDEAAHAWNDVNSALDSVQQVVNEEYAAKEA 311
Query: 478 VQKATMALSLAEARLQVATESLDLAKGGNDSPETSIDSEGEIDGKEEQEVLLVAQEDIKE 537
VQKATMALSLAEARLQVA ESL+LAK GNDSPETS +SEGEIDGKE+QEVLLVAQEDIKE
Sbjct: 312 VQKATMALSLAEARLQVAIESLELAK-GNDSPETSTESEGEIDGKEKQEVLLVAQEDIKE 371
Query: 538 CRANLEICDAELKRVQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAF 597
C A+LE CDAELKR+QSKKEELQKEVDRL ELAEK QL ALKAEE+VANIMLLAEQAVAF
Sbjct: 372 CCASLETCDAELKRLQSKKEELQKEVDRLTELAEKEQLIALKAEEEVANIMLLAEQAVAF 431
Query: 598 ELEAAQRVSDAEIVLQKVEKSLSNSFVDTSETTQGSNVIEEVVNEDNKAVLEISGDIAVE 657
ELEAAQRVSDAEIVLQ+VEKS+SNSFVDTSETTQGSN+IEEV NE+NKAVLEISGDIAVE
Sbjct: 432 ELEAAQRVSDAEIVLQRVEKSISNSFVDTSETTQGSNIIEEVANENNKAVLEISGDIAVE 491
Query: 658 MDRDLPLSGDSLAVKSLPGSFSDSEDSDQAYDLSDNDNGKLSSDSFKEVEAGAEKSVLSQ 717
MDRDLPLSGDSL +KSLPGSFSDSEDSDQ YDLSD++NGKL+SDS KEVE+GA KS+LSQ
Sbjct: 492 MDRDLPLSGDSLVIKSLPGSFSDSEDSDQPYDLSDSENGKLNSDSLKEVESGAAKSILSQ 551
Query: 718 AKKQETQKDLTREGSPLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALV----LDFTKK 777
AKKQETQKDLTREGS LNSPKALLKKSSRFFSASFFSF+ DG EFTPALV LD TKK
Sbjct: 552 AKKQETQKDLTREGSSLNSPKALLKKSSRFFSASFFSFSADGEEFTPALVFQGLLDTTKK 611
Query: 778 QLPKLVVGVVLLGAGITIFSNRAERSSQMFQQPDAVSISTDEVSLNTKPLFQQLRKLPKR 837
QLPKL+VGVVLLGAGI+IF+NRAER S MFQQPD V+ STDEVS NTKPLFQQL KLP++
Sbjct: 612 QLPKLIVGVVLLGAGISIFANRAERKSHMFQQPDVVTTSTDEVSWNTKPLFQQLSKLPQK 671
Query: 838 VKKLIAKIPHQEVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYG 897
VKKLI+KIPHQEVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYG
Sbjct: 672 VKKLISKIPHQEVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYG 731
Query: 898 LSIIRHVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVA 957
LSIIRHVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVG++A
Sbjct: 732 LSIIRHVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGMIA 791
Query: 958 HIVCGQPGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLI 1017
HIVCGQPGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLI
Sbjct: 792 HIVCGQPGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLI 851
Query: 1018 LIPLISPNSSKGGIGFQAIAEALGVAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEI 1077
LIPLISPNSSKGGIGFQAIAEALG+AAVKAIVAIS IIAGGRLLLRPIYKQIAENQNAEI
Sbjct: 852 LIPLISPNSSKGGIGFQAIAEALGLAAVKAIVAISTIIAGGRLLLRPIYKQIAENQNAEI 911
Query: 1078 FSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFM 1137
FSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFM
Sbjct: 912 FSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFM 971
Query: 1138 TVGMSIDPKLLLSNFPVITGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEF 1197
TVGMSIDPKLLLSNFPVI GSLGLLIGGKTILVAL+GRLFGISIISALRVGLLLAPGGEF
Sbjct: 972 TVGMSIDPKLLLSNFPVIMGSLGLLIGGKTILVALIGRLFGISIISALRVGLLLAPGGEF 1031
Query: 1198 AFVAFGEAVNQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVES 1257
AFVAFGEAVNQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIAS FEQHDVRSLLPVES
Sbjct: 1032 AFVAFGEAVNQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASHFEQHDVRSLLPVES 1091
Query: 1258 ETDDLQDHIIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSR 1317
ETDDLQDHII+CGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSR
Sbjct: 1092 ETDDLQDHIILCGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSR 1151
Query: 1318 EVLHKVGAERACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATA 1377
EVLHK+GAERACAAAITLD+PGANYRTVWAL+KYFPNVKTFVRAHDVDHGLNLEKAGATA
Sbjct: 1152 EVLHKIGAERACAAAITLDSPGANYRTVWALNKYFPNVKTFVRAHDVDHGLNLEKAGATA 1211
Query: 1378 VVPETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRI 1437
VVPETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTEL EASGSSLGYGFSRI
Sbjct: 1212 VVPETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELSEASGSSLGYGFSRI 1258
Query: 1438 MSKPKIQTSDSSDENQITEGTL 1455
MSKPKIQTSD SDENQ+TEGTL
Sbjct: 1272 MSKPKIQTSDLSDENQVTEGTL 1258
BLAST of Lag0020772 vs. NCBI nr
Match:
KAG7018984.1 (K(+) efflux antiporter 2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 2050.0 bits (5310), Expect = 0.0e+00
Identity = 1125/1213 (92.75%), Postives = 1159/1213 (95.55%), Query Frame = 0
Query: 249 SC-IWKQNVLCGSDRTYCKTLDQFGPGNVLRNRIIDAKLTGGSRVFYKVPKKRNRIVASI 308
SC IWKQNVLCGS+RT+CKTLDQFG VLR+R +DAKL+GGSRV YKVP KRNRIV+S
Sbjct: 4 SCHIWKQNVLCGSERTHCKTLDQFGLRYVLRSRALDAKLSGGSRVIYKVPNKRNRIVSSS 63
Query: 309 NSNHLSLVCASKFDGALQIFNHKRFLNFNNKIIGRRTRMVNSECQSNDSLAFIDGNGRNV 368
+S HLSLVC SKFDGALQI HKRFLN N GR+ MV+SECQSNDSLAFIDGNGRN+
Sbjct: 64 DSIHLSLVCGSKFDGALQISKHKRFLNCNYN--GRKMGMVHSECQSNDSLAFIDGNGRNM 123
Query: 369 EYVDTSDEGSSSEPTDGVGSAGSKEAGGEAEAVETDAPTVDELRELLQKAMTELEVARLN 428
EYVDT DEGSSS PTDGVGSAGS+EAGGEAEAVET APTVDELRELLQKA+ ELEVARLN
Sbjct: 124 EYVDTGDEGSSSVPTDGVGSAGSREAGGEAEAVETVAPTVDELRELLQKAIKELEVARLN 183
Query: 429 STMFEEKAQKISETAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMALSLA 488
STMFEEKAQKISETAIALQDEA HA NDVNSTLDSVQQIV EEYAAKEAVQKATMALSLA
Sbjct: 184 STMFEEKAQKISETAIALQDEATHARNDVNSTLDSVQQIVKEEYAAKEAVQKATMALSLA 243
Query: 489 EARLQVATESLDLAKGGNDSPETSIDSEGEIDGKEEQEVLLVAQEDIKECRANLEICDAE 548
EARLQVA ES++LAKGGND PETSI+SEGEIDGK+EQEVLLVAQEDIKECRANLE CDAE
Sbjct: 244 EARLQVAIESMELAKGGNDFPETSINSEGEIDGKDEQEVLLVAQEDIKECRANLENCDAE 303
Query: 549 LKRVQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVSDA 608
LKR+QSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVSDA
Sbjct: 304 LKRLQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVSDA 363
Query: 609 EIVLQKVEKSLSNSFVDTSETTQGSNVIEEVVNEDNKAVLEISGDIAVEMDRDLPLSGDS 668
EI LQKVEKSLS+SFVDTSE TQG NVIEEV NEDNKAV EISGDIAVEMDRDLPLSGDS
Sbjct: 364 EIALQKVEKSLSSSFVDTSEITQGLNVIEEVENEDNKAVPEISGDIAVEMDRDLPLSGDS 423
Query: 669 LAVKSLPGSFSDSEDSDQAYDLSDNDNGKLSSDSFKEVEAGAEKSVLSQAKKQETQKDLT 728
L +KSLPGSFSDSEDSDQ Y LSD++NGKLSSDS KEVE+GAEKS+LSQAKK ETQKDLT
Sbjct: 424 LVIKSLPGSFSDSEDSDQPYYLSDSENGKLSSDSSKEVESGAEKSILSQAKKPETQKDLT 483
Query: 729 REGSPLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALV----LDFTKKQLPKLVVGVVL 788
REGSP+NSPKALLKKSSRFFSASFFSF VDGTEFTPALV LD TKKQLPKLVVG VL
Sbjct: 484 REGSPVNSPKALLKKSSRFFSASFFSFAVDGTEFTPALVFQGFLDSTKKQLPKLVVGAVL 543
Query: 789 LGAGITIFSNRAERSSQMFQQPDAVSISTDEVSLNTKPLFQQLRKLPKRVKKLIAKIPHQ 848
GAGI+I +NRAER+S MFQQPD V+ STDEVSLNTKPLFQQLRKLPKRVKKLIAK+PHQ
Sbjct: 544 FGAGISILANRAERNSLMFQQPDVVTTSTDEVSLNTKPLFQQLRKLPKRVKKLIAKLPHQ 603
Query: 849 EVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTK 908
EVNEEEASLLD+LWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTK
Sbjct: 604 EVNEEEASLLDVLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTK 663
Query: 909 AIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPGPAA 968
AIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQ GPAA
Sbjct: 664 AIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQAGPAA 723
Query: 969 IVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSK 1028
IVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSK
Sbjct: 724 IVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSK 783
Query: 1029 GGIGFQAIAEALGVAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILG 1088
GGIGFQAIAEALG+AAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILG
Sbjct: 784 GGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILG 843
Query: 1089 TSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLL 1148
TSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLL
Sbjct: 844 TSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLL 903
Query: 1149 LSNFPVITGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEAVNQ 1208
LSNFPVI GSLGLLIGGKTILVALVGRLFG+SIISALRVGLLLAPGGEFAFVAFGEAVNQ
Sbjct: 904 LSNFPVIMGSLGLLIGGKTILVALVGRLFGVSIISALRVGLLLAPGGEFAFVAFGEAVNQ 963
Query: 1209 GIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIII 1268
GIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIII
Sbjct: 964 GIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIII 1023
Query: 1269 CGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERA 1328
CGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERA
Sbjct: 1024 CGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERA 1083
Query: 1329 CAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQ 1388
CAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQ
Sbjct: 1084 CAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQ 1143
Query: 1389 LAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTSDS 1448
LAAAVLAQAKLP SEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPK+QTSDS
Sbjct: 1144 LAAAVLAQAKLPTSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKVQTSDS 1203
Query: 1449 SDENQITEGTLAI 1457
SDENQ+TEGTLAI
Sbjct: 1204 SDENQVTEGTLAI 1214
BLAST of Lag0020772 vs. NCBI nr
Match:
KAA0048981.1 (K(+) efflux antiporter 2 [Cucumis melo var. makuwa])
HSP 1 Score: 2047.7 bits (5304), Expect = 0.0e+00
Identity = 1123/1252 (89.70%), Postives = 1168/1252 (93.29%), Query Frame = 0
Query: 236 CLIECQQWLNDWQ---------------------SC-IWKQNVLCGSDRTYCKTLDQFGP 295
CL +CQ+WLNDW SC WKQNVL GS+RTYCKTLDQFGP
Sbjct: 12 CLTDCQRWLNDWHEYVIEHWVLERLDSFPGTMDLSCSTWKQNVLRGSERTYCKTLDQFGP 71
Query: 296 GNVLRNRIIDAKLTGGSRVFYKVPKKRNRIVASINSNHLSLVCASKFDGALQIFNHKRFL 355
+VLRNRI DAKLTGGSRV YKVPKKRNRIVAS +SNHLSLVCASKFD ALQIF KRFL
Sbjct: 72 RSVLRNRIFDAKLTGGSRVSYKVPKKRNRIVASSDSNHLSLVCASKFDRALQIFTPKRFL 131
Query: 356 NFNNKIIGRRTRMVNSECQSNDSLAFIDGNGRNVEYVDTSDEGSSSEPTDGVGSAGSKEA 415
N+NNKI GR MV+ ECQ+NDSLAFIDGNGRN+EYV++ DEGSSS P DGVGSAGS+E
Sbjct: 132 NWNNKISGRGMGMVHLECQNNDSLAFIDGNGRNIEYVNSGDEGSSSGPADGVGSAGSREV 191
Query: 416 GGEAEAVETDAPTVDELRELLQKAMTELEVARLNSTMFEEKAQKISETAIALQDEAAHAW 475
GGEAE VET+ PTVDELRELLQKAM ELEVARLNSTMFEE+AQKISE AIALQDEA +AW
Sbjct: 192 GGEAETVETNIPTVDELRELLQKAMKELEVARLNSTMFEERAQKISEAAIALQDEATNAW 251
Query: 476 NDVNSTLDSVQQIVNEEYAAKEAVQKATMALSLAEARLQVATESLDLAKGGNDSPETSID 535
NDVNSTLDSVQQIVNEEY AKEAVQKATMALSLAEARLQVA ESL+LAK G+D PETS+D
Sbjct: 252 NDVNSTLDSVQQIVNEEYVAKEAVQKATMALSLAEARLQVAIESLELAKRGSDFPETSMD 311
Query: 536 SEGEIDGKEEQEVLLVAQEDIKECRANLEICDAELKRVQSKKEELQKEVDRLNELAEKAQ 595
S+G IDGKE+QE LLVAQEDI +CRANLEIC+AEL R+QSKKEELQKEVDRLNELAEKAQ
Sbjct: 312 SDGVIDGKEDQEALLVAQEDITDCRANLEICNAELMRLQSKKEELQKEVDRLNELAEKAQ 371
Query: 596 LNALKAEEDVANIMLLAEQAVAFELEAAQRVSDAEIVLQKVEKSLSNSFVDTSETTQGSN 655
LNALKAEEDVANIMLLAEQAVAFELEAAQRV+DAE LQKVEKSLS+SFVDTS+ TQGSN
Sbjct: 372 LNALKAEEDVANIMLLAEQAVAFELEAAQRVNDAERALQKVEKSLSSSFVDTSDITQGSN 431
Query: 656 VIEEVVNEDNKAVLEISGDIAVEMDRDLPLSGDSLAVKSLPGSFSDSEDSDQAYDLSDND 715
VIEEV NEDNKAVLEISGDIAVEMDR+LPL+GDSLA+KSLPGS SDSE SDQ Y LSD++
Sbjct: 432 VIEEVENEDNKAVLEISGDIAVEMDRELPLNGDSLAIKSLPGSLSDSEGSDQPYYLSDSE 491
Query: 716 NGKLSSDSFKEVEAGAEKSVLSQAKKQETQKDLTREGSPLNSPKALLKKSSRFFSASFFS 775
NGKLSSDS KEVE+GAEKS+LSQ KKQE QKDLTREGSPLNSPKALLKKSSRFFSASFFS
Sbjct: 492 NGKLSSDSAKEVESGAEKSILSQTKKQEIQKDLTREGSPLNSPKALLKKSSRFFSASFFS 551
Query: 776 FTVDGTEFTPALV----LDFTKKQLPKLVVGVVLLGAGITIFSNRAERSSQMFQQPDAVS 835
FTVDGTEFTPALV LD TKKQLPKL+VG VLLGAGI +F+NRAERSSQM QPD V+
Sbjct: 552 FTVDGTEFTPALVFQGLLDSTKKQLPKLIVGAVLLGAGIAVFANRAERSSQMIPQPDVVT 611
Query: 836 ISTDEVSLNTKPLFQQLRKLPKRVKKLIAKIPHQE-----VNEEEASLLDMLWLLLASVI 895
ISTD+VSL+TKPLFQQLRKLPKRVKKLI++IPHQE VNEEEASLLDMLWLLLASVI
Sbjct: 612 ISTDDVSLDTKPLFQQLRKLPKRVKKLISQIPHQEACTLLVNEEEASLLDMLWLLLASVI 671
Query: 896 FVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAEFGVVFLLFNIGLELSVE 955
FVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAEFGVVFLLFNIGLELSVE
Sbjct: 672 FVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAEFGVVFLLFNIGLELSVE 731
Query: 956 RLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPGPAAIVIGNGLALSSTAVVLQVLQE 1015
RLSSMKKYVFGLGSAQVLVTAVVVGLVAH+VCGQ GPAAIVIGNGLALSSTAVVLQVLQE
Sbjct: 732 RLSSMKKYVFGLGSAQVLVTAVVVGLVAHMVCGQAGPAAIVIGNGLALSSTAVVLQVLQE 791
Query: 1016 RGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGVAAVKAIV 1075
RGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALG+AAVKAIV
Sbjct: 792 RGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAAVKAIV 851
Query: 1076 AISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGL 1135
AISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGL
Sbjct: 852 AISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGL 911
Query: 1136 LLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNFPVITGSLGLLIGGKTIL 1195
LLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLL SNFPVI GSLGLLIGGKTIL
Sbjct: 912 LLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLFSNFPVIMGSLGLLIGGKTIL 971
Query: 1196 VALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEAVNQGIMSSQLSSLLFLVVGISMAL 1255
VALVGRLFGISIISA+RVGLLLAPGGEFAFVAFGEAVNQGIMSSQLSSLLFLVVGISMAL
Sbjct: 972 VALVGRLFGISIISAIRVGLLLAPGGEFAFVAFGEAVNQGIMSSQLSSLLFLVVGISMAL 1031
Query: 1256 TPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIIICGFGRVGQIIAQLLSERLIPF 1315
TPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIIICGFGRVGQIIAQLLSERLIPF
Sbjct: 1032 TPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIIICGFGRVGQIIAQLLSERLIPF 1091
Query: 1316 VALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERACAAAITLDTPGANYRTVWALS 1375
VALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERACAAAITLDTPGANYRTVWALS
Sbjct: 1092 VALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERACAAAITLDTPGANYRTVWALS 1151
Query: 1376 KYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPMSEIAATIN 1435
KYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPMSEIAATIN
Sbjct: 1152 KYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPMSEIAATIN 1211
Query: 1436 EFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTSDSSDENQITEGTLAI 1457
EFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTSDSSDENQ+TEGTLAI
Sbjct: 1212 EFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTSDSSDENQVTEGTLAI 1263
BLAST of Lag0020772 vs. NCBI nr
Match:
XP_022980192.1 (K(+) efflux antiporter 2, chloroplastic-like [Cucurbita maxima] >XP_022980193.1 K(+) efflux antiporter 2, chloroplastic-like [Cucurbita maxima])
HSP 1 Score: 2046.9 bits (5302), Expect = 0.0e+00
Identity = 1123/1213 (92.58%), Postives = 1158/1213 (95.47%), Query Frame = 0
Query: 249 SC-IWKQNVLCGSDRTYCKTLDQFGPGNVLRNRIIDAKLTGGSRVFYKVPKKRNRIVASI 308
SC IWKQNVLCGS+RT+CKTLDQFG VLR+R +DAK++GGSRV YKVP KRNRIV+S
Sbjct: 4 SCNIWKQNVLCGSERTHCKTLDQFGLRYVLRSRALDAKMSGGSRVIYKVPNKRNRIVSSS 63
Query: 309 NSNHLSLVCASKFDGALQIFNHKRFLNFNNKIIGRRTRMVNSECQSNDSLAFIDGNGRNV 368
+S HLSLVCASKFDGALQI HKRFLN N GR+ MV+SECQSNDSLAFIDGNGRN+
Sbjct: 64 DSFHLSLVCASKFDGALQISKHKRFLNCNYN--GRKMGMVHSECQSNDSLAFIDGNGRNM 123
Query: 369 EYVDTSDEGSSSEPTDGVGSAGSKEAGGEAEAVETDAPTVDELRELLQKAMTELEVARLN 428
EYVDT DEGSSS PTDGVGSAGS+EAGGE EAVET APTVDELRELLQKAM ELEVARLN
Sbjct: 124 EYVDTGDEGSSSVPTDGVGSAGSREAGGETEAVETVAPTVDELRELLQKAMKELEVARLN 183
Query: 429 STMFEEKAQKISETAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMALSLA 488
STMFEEKAQKISETAIALQDEA HA NDVNSTLDSVQQIVNEEYAAKEAVQKATMALSLA
Sbjct: 184 STMFEEKAQKISETAIALQDEATHARNDVNSTLDSVQQIVNEEYAAKEAVQKATMALSLA 243
Query: 489 EARLQVATESLDLAKGGNDSPETSIDSEGEIDGKEEQEVLLVAQEDIKECRANLEICDAE 548
EARLQVA ES++LAKGGND PETSI+SE EIDGK+EQ+VLLVAQEDIKECRANLE CDAE
Sbjct: 244 EARLQVAIESMELAKGGNDFPETSINSEREIDGKDEQDVLLVAQEDIKECRANLENCDAE 303
Query: 549 LKRVQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVSDA 608
LKR+QSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVSDA
Sbjct: 304 LKRLQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVSDA 363
Query: 609 EIVLQKVEKSLSNSFVDTSETTQGSNVIEEVVNEDNKAVLEISGDIAVEMDRDLPLSGDS 668
EI LQKVEKSLS+SFVDTSE TQG NVIEEV NEDNKAV EISGDIAVEMDRDLPLSGDS
Sbjct: 364 EIALQKVEKSLSSSFVDTSEITQGLNVIEEVENEDNKAVPEISGDIAVEMDRDLPLSGDS 423
Query: 669 LAVKSLPGSFSDSEDSDQAYDLSDNDNGKLSSDSFKEVEAGAEKSVLSQAKKQETQKDLT 728
L +KSLPGSFSDSEDSDQ Y LSD++NGKLSSDS KEVE+GAEKS+LSQAKK ETQKDLT
Sbjct: 424 LVIKSLPGSFSDSEDSDQPYYLSDSENGKLSSDSSKEVESGAEKSILSQAKKPETQKDLT 483
Query: 729 REGSPLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALV----LDFTKKQLPKLVVGVVL 788
REGSP+NSPKALLKKSSRFFSASFFSF VDGTEFTPALV LD TKKQLPKLVVG VL
Sbjct: 484 REGSPVNSPKALLKKSSRFFSASFFSFAVDGTEFTPALVFQGFLDSTKKQLPKLVVGAVL 543
Query: 789 LGAGITIFSNRAERSSQMFQQPDAVSISTDEVSLNTKPLFQQLRKLPKRVKKLIAKIPHQ 848
GAGI I +NRAER+S MFQQPD V+ STDEVSLNTKPLFQQLRKLPKRVKKLIAK+PHQ
Sbjct: 544 FGAGIAILANRAERNSLMFQQPDVVTTSTDEVSLNTKPLFQQLRKLPKRVKKLIAKLPHQ 603
Query: 849 EVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTK 908
EVNEEEASLLD+LWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTK
Sbjct: 604 EVNEEEASLLDVLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTK 663
Query: 909 AIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPGPAA 968
AIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQ GPAA
Sbjct: 664 AIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQAGPAA 723
Query: 969 IVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSK 1028
IVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSK
Sbjct: 724 IVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSK 783
Query: 1029 GGIGFQAIAEALGVAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILG 1088
GGIGFQAIAEALG+AAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILG
Sbjct: 784 GGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILG 843
Query: 1089 TSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLL 1148
TSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLL
Sbjct: 844 TSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLL 903
Query: 1149 LSNFPVITGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEAVNQ 1208
LSNFPVI GSLGLLIGGKTILVALVGRLFG+SIISALRVGLLLAPGGEFAFVAFGEAVNQ
Sbjct: 904 LSNFPVIMGSLGLLIGGKTILVALVGRLFGVSIISALRVGLLLAPGGEFAFVAFGEAVNQ 963
Query: 1209 GIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIII 1268
GIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIII
Sbjct: 964 GIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIII 1023
Query: 1269 CGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERA 1328
CGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERA
Sbjct: 1024 CGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERA 1083
Query: 1329 CAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQ 1388
CAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQ
Sbjct: 1084 CAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQ 1143
Query: 1389 LAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTSDS 1448
LAAAVLAQAKLP SEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPK+QTSDS
Sbjct: 1144 LAAAVLAQAKLPTSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKVQTSDS 1203
Query: 1449 SDENQITEGTLAI 1457
SDENQ++EGTLAI
Sbjct: 1204 SDENQVSEGTLAI 1214
BLAST of Lag0020772 vs. ExPASy Swiss-Prot
Match:
O65272 (K(+) efflux antiporter 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KEA2 PE=1 SV=2)
HSP 1 Score: 1442.9 bits (3734), Expect = 0.0e+00
Identity = 858/1225 (70.04%), Postives = 975/1225 (79.59%), Query Frame = 0
Query: 246 DWQSCIWKQNVLCGSD--RTYCKTLDQFGPGNVLRNRIIDAKL----TGGSRVFYKV--P 305
D+ S + +Q++ G +YC L NR+I AKL GG+R + +
Sbjct: 2 DFASSVQRQSMFHGGADFASYC-----------LPNRMISAKLCPKGLGGTRFWDPMIDS 61
Query: 306 KKRNRIVASIN-SNHLSLVCASKFDGALQIFNHKRFLNFNNKIIGRRTRMVNSECQSNDS 365
K R+ I + N S SL + F+G + + H N +G R CQS+DS
Sbjct: 62 KVRSAIRSKRNVSYRSSLTLNADFNG--RFYGHLLPAKPQNVPLGFRLL-----CQSSDS 121
Query: 366 LAFIDGNGRNVEYVDTSDEGSSSEPTDGVGSAGSKEAGGEAEAVETDAPTVDELRELLQK 425
+ + GN RN+E+ + SD+ + SKE E + AP+++ELR+LL K
Sbjct: 122 VGDLVGNDRNLEFAEGSDDREVT---------FSKEEKDTRE--QDSAPSLEELRDLLNK 181
Query: 426 AMTELEVARLNSTMFEEKAQKISETAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEA 485
A ELEVA LNSTMFEEKAQ+ISE AIAL+DEAA AWNDVN TL+ VQ+ V+EE AKEA
Sbjct: 182 ATKELEVASLNSTMFEEKAQRISEVAIALKDEAASAWNDVNQTLNVVQEAVDEESVAKEA 241
Query: 486 VQKATMALSLAEARLQVATESLDLAKGGNDSPETSIDSEGEIDG-KEEQEVLLVAQEDIK 545
VQKATMALSLAEARLQVA ESL+ A+G N S E+ + DG K+++E LL A+ DIK
Sbjct: 242 VQKATMALSLAEARLQVALESLE-AEGYNTSEESEVR-----DGVKDKEEALLSAKADIK 301
Query: 546 ECRANLEICDAELKRVQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVA 605
EC+ NL C+ +L+R+Q KK+ELQKEVDRLNE AE+AQ++ALKAEEDVANIM+LAEQAVA
Sbjct: 302 ECQENLASCEEQLRRLQVKKDELQKEVDRLNEAAERAQISALKAEEDVANIMVLAEQAVA 361
Query: 606 FELEAAQRVSDAEIVLQKVEKSLSNSFVDTSETTQGS--NVIEEVVNEDNKAVLEISGDI 665
FELEA QRV+DAEI LQ+ EK+L S T ETTQG + +V ED VL D+
Sbjct: 362 FELEATQRVNDAEIALQRAEKTLFGS--QTQETTQGKVLDGKNTIVGEDE--VLSEIVDV 421
Query: 666 AVEMDRDLPLSGDSLAVKSLPGSFSDSEDSDQAYDLSDNDNGKLSSDSFKEVEAGAEKSV 725
+ + +RDL + G S S+ Q+Y+ SDN+NGK ++D KE E AEKS
Sbjct: 422 SHQAERDLVVVGVS------------SDVGTQSYE-SDNENGKPTADFAKEAEGEAEKSK 481
Query: 726 -LSQAKKQETQKDLTREGSPLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALVLDFTKK 785
+ KKQE QKDL RE S N K LKKSSRFF ASFFS DGT +++ K+
Sbjct: 482 NVVLTKKQEVQKDLPRESSSHNGTKTSLKKSSRFFPASFFSSNGDGTATVFESLVESAKQ 541
Query: 786 QLPKLVVGVVLLGAGITIFSNRAERSSQMFQQPDAVSISTDEVSLNTKPLFQQLRKLPKR 845
Q PKL++G LLGAG+ I+SN R++Q+ QQP+ VS S ++VS +TKPL +Q++KLPKR
Sbjct: 542 QWPKLILGFTLLGAGVAIYSNGVGRNNQLPQQPNIVSTSAEDVSSSTKPLIRQMQKLPKR 601
Query: 846 VKKLIAKIPHQEVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYG 905
+KKL+ P QEVNEEEASLLD+LWLLLASVIFVP FQK+PGGSPVLGYLAAGILIGPYG
Sbjct: 602 IKKLLEMFPQQEVNEEEASLLDVLWLLLASVIFVPLFQKIPGGSPVLGYLAAGILIGPYG 661
Query: 906 LSIIRHVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVA 965
LSIIR+VHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTA V+GL+
Sbjct: 662 LSIIRNVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAAVIGLIT 721
Query: 966 HIVCGQPGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLI 1025
H V GQ GPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLI
Sbjct: 722 HYVAGQAGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLI 781
Query: 1026 LIPLISPNSSKGGIGFQAIAEALGVAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEI 1085
LIPLISPNSSKGGIGFQAIAEALG+AA+KA VAI+ IIAGGRLLLRPIYKQIAEN+NAEI
Sbjct: 782 LIPLISPNSSKGGIGFQAIAEALGLAAIKAAVAITGIIAGGRLLLRPIYKQIAENRNAEI 841
Query: 1086 FSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFM 1145
FSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFM
Sbjct: 842 FSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFM 901
Query: 1146 TVGMSIDPKLLLSNFPVITGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEF 1205
TVGMSIDPKLLL+NFP+I G+LGLL+ GKTILV ++G+LFGISIISA+RVGLLLAPGGEF
Sbjct: 902 TVGMSIDPKLLLANFPLIMGTLGLLLVGKTILVVIIGKLFGISIISAVRVGLLLAPGGEF 961
Query: 1206 AFVAFGEAVNQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVES 1265
AFVAFGEAVNQGIM+ QLSSLLFLVVGISMALTPWLAAGGQLIASRFE DVRSLLPVES
Sbjct: 962 AFVAFGEAVNQGIMTPQLSSLLFLVVGISMALTPWLAAGGQLIASRFELQDVRSLLPVES 1021
Query: 1266 ETDDLQDHIIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSR 1325
ETDDLQ HIIICGFGR+GQIIAQLLSERLIPFVALDV SDRVA+GR+LDLPVYFGDAGSR
Sbjct: 1022 ETDDLQGHIIICGFGRIGQIIAQLLSERLIPFVALDVSSDRVAIGRSLDLPVYFGDAGSR 1081
Query: 1326 EVLHKVGAERACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATA 1385
EVLHK+GA+RACAAAI LDTPGANYR VWALSKYFPNVKTFVRAHDVDHGLNLEKAGATA
Sbjct: 1082 EVLHKIGADRACAAAIALDTPGANYRCVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATA 1141
Query: 1386 VVPETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRI 1445
VVPETLEPSLQLAAAVLAQAKLP SEIA TINEFRSRHLSEL ELCEASGSSLGYGFSR
Sbjct: 1142 VVPETLEPSLQLAAAVLAQAKLPTSEIATTINEFRSRHLSELAELCEASGSSLGYGFSRS 1174
Query: 1446 MSKPKIQT-SDSSDENQITEGTLAI 1457
SKPK + S++SD+NQI EGTLAI
Sbjct: 1202 TSKPKPPSPSETSDDNQIIEGTLAI 1174
BLAST of Lag0020772 vs. ExPASy Swiss-Prot
Match:
Q9ZTZ7 (K(+) efflux antiporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KEA1 PE=1 SV=2)
HSP 1 Score: 1436.8 bits (3718), Expect = 0.0e+00
Identity = 833/1183 (70.41%), Postives = 943/1183 (79.71%), Query Frame = 0
Query: 279 NRIIDAK---LTGGSRVFYKVPKKRNRIVASINSNHLSLVCASKFDGALQIFNHKRFLNF 338
NR+I K +T G + + R + S N ++ + +F + + K L
Sbjct: 25 NRLISPKGISITSGDSKVHSCFRLRRNVAQSGTLNLMNACFSGRFYSG-HLHSTKSILG- 84
Query: 339 NNKIIGRRTRMVNSECQSNDSLAFIDGNGRNVEYVDTSDEGSSSEPTDGVGSAGSKEAGG 398
N R CQ ++SL D N + G SSE +D + K+A
Sbjct: 85 NGHQAKRIPFGFRLRCQGHESLGNADSNDHRI--------GESSESSDETEATDLKDA-- 144
Query: 399 EAEAVETDAPTVDELRELLQKAMTELEVARLNSTMFEEKAQKISETAIALQDEAAHAWND 458
VE D +++EL+ELL KA+ ELEVARLNSTMFEEKAQ+ISE AIAL+DEAA AW D
Sbjct: 145 ---RVENDTDSLEELKELLHKAIKELEVARLNSTMFEEKAQRISERAIALKDEAATAWLD 204
Query: 459 VNSTLDSVQQIVNEEYAAKEAVQKATMALSLAEARLQVATESLDLAKGGNDSPETSIDSE 518
VN TLD ++ V EE AKEAVQ ATMALSLAEARLQV ESL+ A GND P S ++E
Sbjct: 205 VNKTLDVIRDTVYEEALAKEAVQTATMALSLAEARLQVIVESLE-AGAGNDIPHVSEETE 264
Query: 519 GEIDGKEEQEVLLVAQEDIKECRANLEICDAELKRVQSKKEELQKEVDRLNELAEKAQLN 578
ID +++E LL A++DIKEC+ NL+ C+++L + SKK+ELQKEVD+LNE AE Q++
Sbjct: 265 ETIDVNDKEEALLAAKDDIKECQVNLDNCESQLSALLSKKDELQKEVDKLNEFAETIQIS 324
Query: 579 ALKAEEDVANIMLLAEQAVAFELEAAQRVSDAEIVLQKVEKSLSNSFVDTSETTQGSNVI 638
+LKAEEDV NIM LAEQAVAFELEA QRV+DAEI LQ+ EKSLS S T E TQG
Sbjct: 325 SLKAEEDVTNIMKLAEQAVAFELEATQRVNDAEIALQRAEKSLSIS--QTPEETQGQLSD 384
Query: 639 EEVVNEDNKAVLEISGDIAVEMDRDLPLSGDSLAVKSLPGSFSD-----SEDSDQAYDLS 698
EE ED + D+ +++++ P GD V+ D ++ Q Y+ S
Sbjct: 385 EETSQEDAMVLSGNVEDVTHQVEKESPKDGDLPVVQITAELVPDIVGQRNKKLTQPYESS 444
Query: 699 DNDNGKLSSDSFKEVEAGAEKSVLS-QAKKQETQKDLTREGSPLNSPKALLKKSSRFFSA 758
D++NGK S +S K VEA +EK ++ Q KKQETQKDL +EGS LNSPKA KSSRFFSA
Sbjct: 445 DHENGKPSVESSKVVEADSEKPKINVQTKKQETQKDLPKEGSSLNSPKASFNKSSRFFSA 504
Query: 759 SFFSFTVDGTEFTPALVLDFTKKQLPKLVVGVVLLGAGITIFSNRAERSSQMFQQPDAVS 818
SFFS DGT ++ K+Q PKLV+G+ LLGAG+T++SN ++Q+ QQPD S
Sbjct: 505 SFFSSNPDGTATVFGSLVGSVKQQWPKLVLGLALLGAGLTLYSNGVGGNNQLLQQPDVTS 564
Query: 819 ISTDEVSLNTKPLFQQLRKLPKRVKKLIAKIPHQEVNEEEASLLDMLWLLLASVIFVPTF 878
ST++VS +TKPL +Q++KLPKR+KKL+ IPHQEVNEEEASL D LWLLLASVIFVP F
Sbjct: 565 TSTEDVSSSTKPLIRQVQKLPKRIKKLLEMIPHQEVNEEEASLFDFLWLLLASVIFVPLF 624
Query: 879 QKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAEFGVVFLLFNIGLELSVERLSSM 938
QK+PGGSPVLGYLAAGILIGPYGLSIIR+VHGT+AIAEFGVVFLLFNIGLELSVERLSSM
Sbjct: 625 QKIPGGSPVLGYLAAGILIGPYGLSIIRNVHGTRAIAEFGVVFLLFNIGLELSVERLSSM 684
Query: 939 KKYVFGLGSAQVLVTAVVVGLVAHIVCGQPGPAAIVIGNGLALSSTAVVLQVLQERGEST 998
KKYVFGLGSAQVLVTA VVGL+AH V GQ GPAAIVIGNGLALSSTAVVLQVLQERGEST
Sbjct: 685 KKYVFGLGSAQVLVTAAVVGLLAHYVAGQAGPAAIVIGNGLALSSTAVVLQVLQERGEST 744
Query: 999 SRHGRATFSVLLFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGVAAVKAIVAISAI 1058
SRHGRA+FSVLLFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALG+AAVKA VAI+AI
Sbjct: 745 SRHGRASFSVLLFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAAVKAAVAITAI 804
Query: 1059 IAGGRLLLRPIYKQIAENQNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAET 1118
IAGGRLLLRPIYKQIAEN+NAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAET
Sbjct: 805 IAGGRLLLRPIYKQIAENRNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAET 864
Query: 1119 EFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNFPVITGSLGLLIGGKTILVALVG 1178
EFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNFPVI G+LGLLI GKT+LV ++G
Sbjct: 865 EFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNFPVIVGTLGLLIVGKTMLVVIMG 924
Query: 1179 RLFGISIISALRVGLLLAPGGEFAFVAFGEAVNQGIMSSQLSSLLFLVVGISMALTPWLA 1238
+LFGISIISA+RVGLLLAPGGEFAFVAFGEAVNQGIMS QLSSLLFLVVGISMA+TPWLA
Sbjct: 925 KLFGISIISAIRVGLLLAPGGEFAFVAFGEAVNQGIMSPQLSSLLFLVVGISMAITPWLA 984
Query: 1239 AGGQLIASRFEQHDVRSLLPVESETDDLQDHIIICGFGRVGQIIAQLLSERLIPFVALDV 1298
AGGQLIASRFE HDVRSLLPVESETDDLQ HIIICGFGRVGQIIAQLLSERLIPFVALDV
Sbjct: 985 AGGQLIASRFELHDVRSLLPVESETDDLQGHIIICGFGRVGQIIAQLLSERLIPFVALDV 1044
Query: 1299 RSDRVAVGRALDLPVYFGDAGSREVLHKVGAERACAAAITLDTPGANYRTVWALSKYFPN 1358
SDRV +GR+LDLPVYFGDAGS+EVLHK+GA RACAA + LD PGANYR VWALSK++PN
Sbjct: 1045 SSDRVTIGRSLDLPVYFGDAGSKEVLHKIGAGRACAAVVALDAPGANYRCVWALSKFYPN 1104
Query: 1359 VKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSR 1418
VKTFVRAHDV HGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLP SEIA TINEFR+R
Sbjct: 1105 VKTFVRAHDVVHGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPTSEIANTINEFRTR 1164
Query: 1419 HLSELTELCEASGSSLGYGFSRIMSKPKIQTSDSSDENQITEG 1453
HLSELTELCEASGSSLGYG+SR SKPK Q SD+S +NQI EG
Sbjct: 1165 HLSELTELCEASGSSLGYGYSR-TSKPKPQPSDASGDNQIIEG 1188
BLAST of Lag0020772 vs. ExPASy Swiss-Prot
Match:
Q9M0Z3 (K(+) efflux antiporter 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KEA3 PE=1 SV=2)
HSP 1 Score: 335.5 bits (859), Expect = 3.0e-90
Identity = 223/601 (37.10%), Postives = 333/601 (55.41%), Query Frame = 0
Query: 854 DMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAEFGVVFL 913
D L L+ +VI VP F+ L SP+LG+ AG+++ +GL IR++ K ++E+G++FL
Sbjct: 107 DTLTFLMVTVIIVPAFRILK-ASPILGFFFAGVVLNQFGL--IRNLTDVKVLSEWGILFL 166
Query: 914 LFNIGLELSVERLSSMKKYVFGLGSAQVLVT-------------AVVVGLVAHIVCGQPG 973
LF +GLELS+ RL ++ K+ FG+G QVL+ A+ ++ + +P
Sbjct: 167 LFEMGLELSLARLKALAKFAFGMGLTQVLLCTAAFTAFELPPNGAIGTKILEFLFHSRPD 226
Query: 974 -------PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLIL 1033
A+VIG L+LSS+A VLQ+L E+GE +R G AT +LL QD+AVV LL++
Sbjct: 227 LVNIRSIDEAVVIGAALSLSSSAFVLQLLAEKGELPTRFGSATLGILLLQDIAVVPLLVI 286
Query: 1034 IPLISPNSSKGGIGFQAIAEALGVAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIF 1093
+P++ IG ++I L + KA+ + + GG+ LR I++ +AE +++E F
Sbjct: 287 LPVLESQD----IGGESIWPMLAKESAKALGGLGILSLGGKFFLRRIFEVVAETRSSEAF 346
Query: 1094 SANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMT 1153
A LL + GTSL+T G S LGAFLAG LLAET F Q+E+DI P+RGLLLGLFF+T
Sbjct: 347 VALCLLTVAGTSLVTQWLGFSDTLGAFLAGALLAETNFRTQIEADIRPFRGLLLGLFFVT 406
Query: 1154 VGMSIDPKLLLSNFPVITGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFA 1213
G SID ++L +P + LG LI KT+++ +G G++I ++RVG LL+ GGEFA
Sbjct: 407 TGTSIDMEVLFREWPNVLSLLGGLIVIKTLIITAIGPRVGLTIQESVRVGFLLSQGGEFA 466
Query: 1214 FVAFGEAVNQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESE 1273
FV F A G++ ++L+ LL +VV +SMALTP+L G+ A ++ E
Sbjct: 467 FVVFSLANRLGVLPNELNKLLIIVVVLSMALTPYLNQLGRKAADFLDERLDPGEKIGEDV 526
Query: 1274 TDDLQDHIIICGFGRVGQIIAQLLSERLI--------PFVALDVRSDRVAVGRALDLPVY 1333
D+ + I+I GFG++GQ++A LS L+ P++ D+ V R L P+
Sbjct: 527 NFDVSESIVIIGFGQMGQVLANFLSTPLVSDSDLVGWPYIGFDLNPAVVKESRKLGFPIL 586
Query: 1334 FGDAGSREVLHKVGAERACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNL 1393
+GD VL G A I V L FP + RA D+ H L L
Sbjct: 587 YGDGSRPSVLQSAGVSSPKAIMIMYKGKKRTTEAVQRLRLAFPGSPIYARAQDLPHLLEL 646
Query: 1394 EKAGATAVVPETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSL 1427
+KAGAT + E E SLQL + +L + +++ FR + E AS ++
Sbjct: 647 KKAGATDAILENAETSLQLGSKLLTGFGVMSDDVSFLSKVFRDSMEIQAQEEITASETNA 700
BLAST of Lag0020772 vs. ExPASy Swiss-Prot
Match:
Q0ZAH7 (Putative K(+) efflux antiporter KefB OS=Alkalimonas amylolytica OX=152573 GN=kefB PE=3 SV=1)
HSP 1 Score: 304.7 bits (779), Expect = 5.7e-81
Identity = 208/585 (35.56%), Postives = 317/585 (54.19%), Query Frame = 0
Query: 849 EASLLDMLWLLLASVIFVPTFQK--LPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIA 908
+ +L+ +L+LL V V FQ+ +P + YL G+L+G + + IA
Sbjct: 4 DGTLIQLLFLLGFMVFMVMLFQRAHIPAS---IAYLLVGVLLGAHTAGPVISEGYIHKIA 63
Query: 909 EFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPGPAAIVI 968
EFG+VFLLF IGL S +++ ++ + GLG+AQV +T ++V L+ + G A VI
Sbjct: 64 EFGIVFLLFTIGLRFSWQQIYQLRHTILGLGTAQVGLTTLLVALLLWAM-GVASVVAFVI 123
Query: 969 GNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSKGGI 1028
G A SST ++ + L E+GE SRHGR S+ +FQD+ V +I+IP++ G
Sbjct: 124 GAVFAQSSTTIISKQLLEQGEDQSRHGRLGISLSVFQDITAVPFIIVIPVL------GVA 183
Query: 1029 GFQAIAEALGVAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILGTSL 1088
Q IA LG+A KA++A + ++ GR LLR ++ +++ + +AE+F+ LLV L +
Sbjct: 184 MAQDIASTLGMALFKAVLATALVVLVGRYLLRHLFHRVSSSDSAELFTLTVLLVCLAAAW 243
Query: 1089 LTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSN 1148
LT GLSMA GAFLAG+++ ETEF LQVE+ I P+R +LLG+FF+++GM +DP LL
Sbjct: 244 LTQSLGLSMAFGAFLAGMVMGETEFKLQVEAAIRPFRDVLLGIFFVSIGMLLDPMLLPEI 303
Query: 1149 FPVITGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEAVNQGIM 1208
+ LL+ K +LV + G+++ +A R GL+LA GGEF F A+ G +
Sbjct: 304 GHIALAGALLLLLIKIVLVTALVLATGVALETAFRTGLILAVGGEFGFALLALALEGGTL 363
Query: 1209 SSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDD------LQDH 1268
S+ S ++ V +SM L +L L SRF V SE D LQ H
Sbjct: 364 DSRSSQIILTSVLLSMMLAVFLIR-YNLQLSRFVVGRWIGQAAVSSEPFDDIEQHGLQQH 423
Query: 1269 IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA 1328
++I GFGR+GQ +AQ L + +P++ LD+ + RV R D+PV++ D+ + L VG
Sbjct: 424 VVIAGFGRIGQGVAQFLQKEQVPYIGLDLDAARVKNARLADVPVFYADSTDPDTLIAVGL 483
Query: 1329 ERACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP 1388
+A I+ + A T+ P++ VR D H L +AGAT V+PET+E
Sbjct: 484 AKARLLVISHEDLSAALTTLRHARTLHPDLPVIVRTRDESHVAELRQAGATEVIPETIEA 543
Query: 1389 SLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSL 1426
+ L + VL +P + + E R L + S L
Sbjct: 544 GMMLTSHVLLMLNVPARRVNQLVQEQRINRYQLLRQQFRGSADLL 577
BLAST of Lag0020772 vs. ExPASy Swiss-Prot
Match:
A9MN27 (Glutathione-regulated potassium-efflux system protein KefB OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) OX=41514 GN=kefB PE=3 SV=1)
HSP 1 Score: 304.7 bits (779), Expect = 5.7e-81
Identity = 213/564 (37.77%), Postives = 324/564 (57.45%), Query Frame = 0
Query: 859 LLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAEFGVVFLLFNIG 918
L A+V+ VP +L G VLGYL AGI IGP+GL I V +E GVVFL+F IG
Sbjct: 14 LFAAVVAVPLAARL-GIGAVLGYLLAGIAIGPWGLGFISDVDEILHFSELGVVFLMFIIG 73
Query: 919 LELSVERLSSMKKYVFGLGSAQVLVT-AVVVGLVAHIVCGQPGPAAIVIGNGLALSSTAV 978
LEL+ RL +++ +FG+G+AQVL++ AV+ GL+ ++ AAIV G GLA+SSTA+
Sbjct: 74 LELNPSRLWQLRRSIFGVGAAQVLLSAAVLAGLL--MLADFLWQAAIVGGIGLAMSSTAM 133
Query: 979 VLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGV 1038
LQ+++E+G + S G+ FSVLLFQDLAV+ L L+PL++ ++ + F+ +G+
Sbjct: 134 ALQLMREKGMNRSESGQLGFSVLLFQDLAVIPALALVPLLAGSADEHFDWFK-----VGM 193
Query: 1039 AAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILGTSLLTARAGLSMAL 1098
++A + ++ GGR LLRP+++ IA + E+F+A TLL++LG +L GLSMAL
Sbjct: 194 ----KVLAFAVMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLGAALFMDALGLSMAL 253
Query: 1099 GAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNFPVITGSLGLL 1158
G F+AG+LLAE+E+ ++E+ I P++GLLLGLFF++VGMS++ +L ++ + S+ +L
Sbjct: 254 GTFIAGVLLAESEYRHELENAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVAASVVIL 313
Query: 1159 IGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEAVNQGIMSSQLSSLLFLV 1218
+ K + + L+ RL+GI ++ +L+ GGEFAFV F A +Q + +LL +
Sbjct: 314 VVIKMLALYLLARLYGIRSSERMQFASVLSQGGEFAFVLFSTASSQRLFQGDQMALLLVA 373
Query: 1219 VGISMALTPWLAAGGQLIASRF----EQHDVRSLLPVESETDDLQDHIIICGFGRVGQII 1278
V +SM TP L G SR E+HD + + +D + +I+ GFGR GQ+I
Sbjct: 374 VTLSMMTTPLLMKGIDKWLSRRLNGPEEHDEKPWV------EDDKPQVIVVGFGRFGQVI 433
Query: 1279 AQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERACAAAITLDTP 1338
A+LL + L+ V + R VY+GDA E+L GAE A + IT + P
Sbjct: 434 ARLLMANKVRITVLERDIGAVNLMRKYGYKVYYGDATQVELLRSAGAEAAESIVITCNEP 493
Query: 1339 GANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAAVLAQAK 1398
+ V ++FP++ RA L +AG T ET +L+L L
Sbjct: 494 EDTMKLVELCQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVSLG 553
Query: 1399 LPMSEIAATINEFRSRHLSELTEL 1418
+ + FR + L EL
Sbjct: 554 MHPHQAQRAQLHFRRLDMRMLREL 559
BLAST of Lag0020772 vs. ExPASy TrEMBL
Match:
A0A5A7U5W1 (K(+) efflux antiporter 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G002050 PE=4 SV=1)
HSP 1 Score: 2047.7 bits (5304), Expect = 0.0e+00
Identity = 1123/1252 (89.70%), Postives = 1168/1252 (93.29%), Query Frame = 0
Query: 236 CLIECQQWLNDWQ---------------------SC-IWKQNVLCGSDRTYCKTLDQFGP 295
CL +CQ+WLNDW SC WKQNVL GS+RTYCKTLDQFGP
Sbjct: 12 CLTDCQRWLNDWHEYVIEHWVLERLDSFPGTMDLSCSTWKQNVLRGSERTYCKTLDQFGP 71
Query: 296 GNVLRNRIIDAKLTGGSRVFYKVPKKRNRIVASINSNHLSLVCASKFDGALQIFNHKRFL 355
+VLRNRI DAKLTGGSRV YKVPKKRNRIVAS +SNHLSLVCASKFD ALQIF KRFL
Sbjct: 72 RSVLRNRIFDAKLTGGSRVSYKVPKKRNRIVASSDSNHLSLVCASKFDRALQIFTPKRFL 131
Query: 356 NFNNKIIGRRTRMVNSECQSNDSLAFIDGNGRNVEYVDTSDEGSSSEPTDGVGSAGSKEA 415
N+NNKI GR MV+ ECQ+NDSLAFIDGNGRN+EYV++ DEGSSS P DGVGSAGS+E
Sbjct: 132 NWNNKISGRGMGMVHLECQNNDSLAFIDGNGRNIEYVNSGDEGSSSGPADGVGSAGSREV 191
Query: 416 GGEAEAVETDAPTVDELRELLQKAMTELEVARLNSTMFEEKAQKISETAIALQDEAAHAW 475
GGEAE VET+ PTVDELRELLQKAM ELEVARLNSTMFEE+AQKISE AIALQDEA +AW
Sbjct: 192 GGEAETVETNIPTVDELRELLQKAMKELEVARLNSTMFEERAQKISEAAIALQDEATNAW 251
Query: 476 NDVNSTLDSVQQIVNEEYAAKEAVQKATMALSLAEARLQVATESLDLAKGGNDSPETSID 535
NDVNSTLDSVQQIVNEEY AKEAVQKATMALSLAEARLQVA ESL+LAK G+D PETS+D
Sbjct: 252 NDVNSTLDSVQQIVNEEYVAKEAVQKATMALSLAEARLQVAIESLELAKRGSDFPETSMD 311
Query: 536 SEGEIDGKEEQEVLLVAQEDIKECRANLEICDAELKRVQSKKEELQKEVDRLNELAEKAQ 595
S+G IDGKE+QE LLVAQEDI +CRANLEIC+AEL R+QSKKEELQKEVDRLNELAEKAQ
Sbjct: 312 SDGVIDGKEDQEALLVAQEDITDCRANLEICNAELMRLQSKKEELQKEVDRLNELAEKAQ 371
Query: 596 LNALKAEEDVANIMLLAEQAVAFELEAAQRVSDAEIVLQKVEKSLSNSFVDTSETTQGSN 655
LNALKAEEDVANIMLLAEQAVAFELEAAQRV+DAE LQKVEKSLS+SFVDTS+ TQGSN
Sbjct: 372 LNALKAEEDVANIMLLAEQAVAFELEAAQRVNDAERALQKVEKSLSSSFVDTSDITQGSN 431
Query: 656 VIEEVVNEDNKAVLEISGDIAVEMDRDLPLSGDSLAVKSLPGSFSDSEDSDQAYDLSDND 715
VIEEV NEDNKAVLEISGDIAVEMDR+LPL+GDSLA+KSLPGS SDSE SDQ Y LSD++
Sbjct: 432 VIEEVENEDNKAVLEISGDIAVEMDRELPLNGDSLAIKSLPGSLSDSEGSDQPYYLSDSE 491
Query: 716 NGKLSSDSFKEVEAGAEKSVLSQAKKQETQKDLTREGSPLNSPKALLKKSSRFFSASFFS 775
NGKLSSDS KEVE+GAEKS+LSQ KKQE QKDLTREGSPLNSPKALLKKSSRFFSASFFS
Sbjct: 492 NGKLSSDSAKEVESGAEKSILSQTKKQEIQKDLTREGSPLNSPKALLKKSSRFFSASFFS 551
Query: 776 FTVDGTEFTPALV----LDFTKKQLPKLVVGVVLLGAGITIFSNRAERSSQMFQQPDAVS 835
FTVDGTEFTPALV LD TKKQLPKL+VG VLLGAGI +F+NRAERSSQM QPD V+
Sbjct: 552 FTVDGTEFTPALVFQGLLDSTKKQLPKLIVGAVLLGAGIAVFANRAERSSQMIPQPDVVT 611
Query: 836 ISTDEVSLNTKPLFQQLRKLPKRVKKLIAKIPHQE-----VNEEEASLLDMLWLLLASVI 895
ISTD+VSL+TKPLFQQLRKLPKRVKKLI++IPHQE VNEEEASLLDMLWLLLASVI
Sbjct: 612 ISTDDVSLDTKPLFQQLRKLPKRVKKLISQIPHQEACTLLVNEEEASLLDMLWLLLASVI 671
Query: 896 FVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAEFGVVFLLFNIGLELSVE 955
FVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAEFGVVFLLFNIGLELSVE
Sbjct: 672 FVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAEFGVVFLLFNIGLELSVE 731
Query: 956 RLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPGPAAIVIGNGLALSSTAVVLQVLQE 1015
RLSSMKKYVFGLGSAQVLVTAVVVGLVAH+VCGQ GPAAIVIGNGLALSSTAVVLQVLQE
Sbjct: 732 RLSSMKKYVFGLGSAQVLVTAVVVGLVAHMVCGQAGPAAIVIGNGLALSSTAVVLQVLQE 791
Query: 1016 RGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGVAAVKAIV 1075
RGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALG+AAVKAIV
Sbjct: 792 RGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAAVKAIV 851
Query: 1076 AISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGL 1135
AISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGL
Sbjct: 852 AISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGL 911
Query: 1136 LLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNFPVITGSLGLLIGGKTIL 1195
LLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLL SNFPVI GSLGLLIGGKTIL
Sbjct: 912 LLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLFSNFPVIMGSLGLLIGGKTIL 971
Query: 1196 VALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEAVNQGIMSSQLSSLLFLVVGISMAL 1255
VALVGRLFGISIISA+RVGLLLAPGGEFAFVAFGEAVNQGIMSSQLSSLLFLVVGISMAL
Sbjct: 972 VALVGRLFGISIISAIRVGLLLAPGGEFAFVAFGEAVNQGIMSSQLSSLLFLVVGISMAL 1031
Query: 1256 TPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIIICGFGRVGQIIAQLLSERLIPF 1315
TPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIIICGFGRVGQIIAQLLSERLIPF
Sbjct: 1032 TPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIIICGFGRVGQIIAQLLSERLIPF 1091
Query: 1316 VALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERACAAAITLDTPGANYRTVWALS 1375
VALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERACAAAITLDTPGANYRTVWALS
Sbjct: 1092 VALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERACAAAITLDTPGANYRTVWALS 1151
Query: 1376 KYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPMSEIAATIN 1435
KYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPMSEIAATIN
Sbjct: 1152 KYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPMSEIAATIN 1211
Query: 1436 EFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTSDSSDENQITEGTLAI 1457
EFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTSDSSDENQ+TEGTLAI
Sbjct: 1212 EFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTSDSSDENQVTEGTLAI 1263
BLAST of Lag0020772 vs. ExPASy TrEMBL
Match:
A0A6J1IYK3 (K(+) efflux antiporter 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111479651 PE=4 SV=1)
HSP 1 Score: 2046.9 bits (5302), Expect = 0.0e+00
Identity = 1123/1213 (92.58%), Postives = 1158/1213 (95.47%), Query Frame = 0
Query: 249 SC-IWKQNVLCGSDRTYCKTLDQFGPGNVLRNRIIDAKLTGGSRVFYKVPKKRNRIVASI 308
SC IWKQNVLCGS+RT+CKTLDQFG VLR+R +DAK++GGSRV YKVP KRNRIV+S
Sbjct: 4 SCNIWKQNVLCGSERTHCKTLDQFGLRYVLRSRALDAKMSGGSRVIYKVPNKRNRIVSSS 63
Query: 309 NSNHLSLVCASKFDGALQIFNHKRFLNFNNKIIGRRTRMVNSECQSNDSLAFIDGNGRNV 368
+S HLSLVCASKFDGALQI HKRFLN N GR+ MV+SECQSNDSLAFIDGNGRN+
Sbjct: 64 DSFHLSLVCASKFDGALQISKHKRFLNCNYN--GRKMGMVHSECQSNDSLAFIDGNGRNM 123
Query: 369 EYVDTSDEGSSSEPTDGVGSAGSKEAGGEAEAVETDAPTVDELRELLQKAMTELEVARLN 428
EYVDT DEGSSS PTDGVGSAGS+EAGGE EAVET APTVDELRELLQKAM ELEVARLN
Sbjct: 124 EYVDTGDEGSSSVPTDGVGSAGSREAGGETEAVETVAPTVDELRELLQKAMKELEVARLN 183
Query: 429 STMFEEKAQKISETAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMALSLA 488
STMFEEKAQKISETAIALQDEA HA NDVNSTLDSVQQIVNEEYAAKEAVQKATMALSLA
Sbjct: 184 STMFEEKAQKISETAIALQDEATHARNDVNSTLDSVQQIVNEEYAAKEAVQKATMALSLA 243
Query: 489 EARLQVATESLDLAKGGNDSPETSIDSEGEIDGKEEQEVLLVAQEDIKECRANLEICDAE 548
EARLQVA ES++LAKGGND PETSI+SE EIDGK+EQ+VLLVAQEDIKECRANLE CDAE
Sbjct: 244 EARLQVAIESMELAKGGNDFPETSINSEREIDGKDEQDVLLVAQEDIKECRANLENCDAE 303
Query: 549 LKRVQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVSDA 608
LKR+QSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVSDA
Sbjct: 304 LKRLQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVSDA 363
Query: 609 EIVLQKVEKSLSNSFVDTSETTQGSNVIEEVVNEDNKAVLEISGDIAVEMDRDLPLSGDS 668
EI LQKVEKSLS+SFVDTSE TQG NVIEEV NEDNKAV EISGDIAVEMDRDLPLSGDS
Sbjct: 364 EIALQKVEKSLSSSFVDTSEITQGLNVIEEVENEDNKAVPEISGDIAVEMDRDLPLSGDS 423
Query: 669 LAVKSLPGSFSDSEDSDQAYDLSDNDNGKLSSDSFKEVEAGAEKSVLSQAKKQETQKDLT 728
L +KSLPGSFSDSEDSDQ Y LSD++NGKLSSDS KEVE+GAEKS+LSQAKK ETQKDLT
Sbjct: 424 LVIKSLPGSFSDSEDSDQPYYLSDSENGKLSSDSSKEVESGAEKSILSQAKKPETQKDLT 483
Query: 729 REGSPLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALV----LDFTKKQLPKLVVGVVL 788
REGSP+NSPKALLKKSSRFFSASFFSF VDGTEFTPALV LD TKKQLPKLVVG VL
Sbjct: 484 REGSPVNSPKALLKKSSRFFSASFFSFAVDGTEFTPALVFQGFLDSTKKQLPKLVVGAVL 543
Query: 789 LGAGITIFSNRAERSSQMFQQPDAVSISTDEVSLNTKPLFQQLRKLPKRVKKLIAKIPHQ 848
GAGI I +NRAER+S MFQQPD V+ STDEVSLNTKPLFQQLRKLPKRVKKLIAK+PHQ
Sbjct: 544 FGAGIAILANRAERNSLMFQQPDVVTTSTDEVSLNTKPLFQQLRKLPKRVKKLIAKLPHQ 603
Query: 849 EVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTK 908
EVNEEEASLLD+LWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTK
Sbjct: 604 EVNEEEASLLDVLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTK 663
Query: 909 AIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPGPAA 968
AIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQ GPAA
Sbjct: 664 AIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQAGPAA 723
Query: 969 IVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSK 1028
IVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSK
Sbjct: 724 IVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSK 783
Query: 1029 GGIGFQAIAEALGVAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILG 1088
GGIGFQAIAEALG+AAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILG
Sbjct: 784 GGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILG 843
Query: 1089 TSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLL 1148
TSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLL
Sbjct: 844 TSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLL 903
Query: 1149 LSNFPVITGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEAVNQ 1208
LSNFPVI GSLGLLIGGKTILVALVGRLFG+SIISALRVGLLLAPGGEFAFVAFGEAVNQ
Sbjct: 904 LSNFPVIMGSLGLLIGGKTILVALVGRLFGVSIISALRVGLLLAPGGEFAFVAFGEAVNQ 963
Query: 1209 GIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIII 1268
GIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIII
Sbjct: 964 GIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIII 1023
Query: 1269 CGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERA 1328
CGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERA
Sbjct: 1024 CGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERA 1083
Query: 1329 CAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQ 1388
CAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQ
Sbjct: 1084 CAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQ 1143
Query: 1389 LAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTSDS 1448
LAAAVLAQAKLP SEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPK+QTSDS
Sbjct: 1144 LAAAVLAQAKLPTSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKVQTSDS 1203
Query: 1449 SDENQITEGTLAI 1457
SDENQ++EGTLAI
Sbjct: 1204 SDENQVSEGTLAI 1214
BLAST of Lag0020772 vs. ExPASy TrEMBL
Match:
A0A6J1E9G2 (K(+) efflux antiporter 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111431943 PE=4 SV=1)
HSP 1 Score: 2046.2 bits (5300), Expect = 0.0e+00
Identity = 1123/1213 (92.58%), Postives = 1156/1213 (95.30%), Query Frame = 0
Query: 249 SC-IWKQNVLCGSDRTYCKTLDQFGPGNVLRNRIIDAKLTGGSRVFYKVPKKRNRIVASI 308
SC IWKQNVLCGS+RT+CKTLDQFG VLR+R +DAKL+GGSRV YKVP RNRIV+S
Sbjct: 4 SCHIWKQNVLCGSERTHCKTLDQFGLRYVLRSRALDAKLSGGSRVIYKVPNNRNRIVSSS 63
Query: 309 NSNHLSLVCASKFDGALQIFNHKRFLNFNNKIIGRRTRMVNSECQSNDSLAFIDGNGRNV 368
+S HLSLVC SKFDGALQI HKRFLN N GR+ MV+SECQSNDSLAFIDGNGRN+
Sbjct: 64 DSIHLSLVCGSKFDGALQISKHKRFLNCNYN--GRKMGMVHSECQSNDSLAFIDGNGRNM 123
Query: 369 EYVDTSDEGSSSEPTDGVGSAGSKEAGGEAEAVETDAPTVDELRELLQKAMTELEVARLN 428
EYVDT DEGSSS PTDGVGSAGS+EAGGEAEAVET APTVDELRELLQKA+ ELEVARLN
Sbjct: 124 EYVDTGDEGSSSVPTDGVGSAGSREAGGEAEAVETVAPTVDELRELLQKAIKELEVARLN 183
Query: 429 STMFEEKAQKISETAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMALSLA 488
STMFEEKAQKISETAIALQDEA HA NDVNSTLDSVQQIV EEYAAKEAVQKATMALSLA
Sbjct: 184 STMFEEKAQKISETAIALQDEATHARNDVNSTLDSVQQIVKEEYAAKEAVQKATMALSLA 243
Query: 489 EARLQVATESLDLAKGGNDSPETSIDSEGEIDGKEEQEVLLVAQEDIKECRANLEICDAE 548
EARLQVA ES++LAKGGND PETSI+SEGEIDGK+EQEVLLVAQEDIKECRANLE CDAE
Sbjct: 244 EARLQVAIESMELAKGGNDFPETSINSEGEIDGKDEQEVLLVAQEDIKECRANLENCDAE 303
Query: 549 LKRVQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVSDA 608
LKR+QSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVSDA
Sbjct: 304 LKRLQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVSDA 363
Query: 609 EIVLQKVEKSLSNSFVDTSETTQGSNVIEEVVNEDNKAVLEISGDIAVEMDRDLPLSGDS 668
EI LQKVEKSLS+SFVDTSE TQG NVIEEV NEDNKAV EISGDIAVEMDRDLPLSGDS
Sbjct: 364 EIALQKVEKSLSSSFVDTSEITQGLNVIEEVENEDNKAVPEISGDIAVEMDRDLPLSGDS 423
Query: 669 LAVKSLPGSFSDSEDSDQAYDLSDNDNGKLSSDSFKEVEAGAEKSVLSQAKKQETQKDLT 728
L +KSLPGSFSDSEDSDQ Y LSD++NG+LSSDS KEVE+GAEKS+LSQAKK ETQKDLT
Sbjct: 424 LVIKSLPGSFSDSEDSDQPYYLSDSENGRLSSDSSKEVESGAEKSILSQAKKPETQKDLT 483
Query: 729 REGSPLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALV----LDFTKKQLPKLVVGVVL 788
REGSP+NSPKALLKKSSRFFSASFFSF VDGTEFTPALV LD TKKQLPKLVVG VL
Sbjct: 484 REGSPVNSPKALLKKSSRFFSASFFSFAVDGTEFTPALVFQGFLDSTKKQLPKLVVGAVL 543
Query: 789 LGAGITIFSNRAERSSQMFQQPDAVSISTDEVSLNTKPLFQQLRKLPKRVKKLIAKIPHQ 848
GAGI I NRAER+S MFQQPD V+ STDEVSLNTKPLFQQLRKLPKRVKKLIAK+PHQ
Sbjct: 544 FGAGIAILGNRAERNSLMFQQPDVVTTSTDEVSLNTKPLFQQLRKLPKRVKKLIAKLPHQ 603
Query: 849 EVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTK 908
EVNEEEASLLD+LWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTK
Sbjct: 604 EVNEEEASLLDVLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTK 663
Query: 909 AIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPGPAA 968
AIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQ GPAA
Sbjct: 664 AIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQAGPAA 723
Query: 969 IVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSK 1028
IVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSK
Sbjct: 724 IVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSK 783
Query: 1029 GGIGFQAIAEALGVAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILG 1088
GGIGFQAIAEALG+AAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILG
Sbjct: 784 GGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILG 843
Query: 1089 TSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLL 1148
TSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLL
Sbjct: 844 TSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLL 903
Query: 1149 LSNFPVITGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEAVNQ 1208
LSNFPVI GSLGLLIGGKTILVALVGRLFG+SIISALRVGLLLAPGGEFAFVAFGEAVNQ
Sbjct: 904 LSNFPVIMGSLGLLIGGKTILVALVGRLFGVSIISALRVGLLLAPGGEFAFVAFGEAVNQ 963
Query: 1209 GIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIII 1268
GIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIII
Sbjct: 964 GIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHIII 1023
Query: 1269 CGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERA 1328
CGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERA
Sbjct: 1024 CGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERA 1083
Query: 1329 CAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQ 1388
CAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQ
Sbjct: 1084 CAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQ 1143
Query: 1389 LAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTSDS 1448
LAAAVLAQAKLP SEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPK+QTSDS
Sbjct: 1144 LAAAVLAQAKLPTSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKVQTSDS 1203
Query: 1449 SDENQITEGTLAI 1457
SDENQ+TEGTLAI
Sbjct: 1204 SDENQVTEGTLAI 1214
BLAST of Lag0020772 vs. ExPASy TrEMBL
Match:
A0A5D3D075 (K(+) efflux antiporter 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004530 PE=4 SV=1)
HSP 1 Score: 2040.8 bits (5286), Expect = 0.0e+00
Identity = 1113/1215 (91.60%), Postives = 1156/1215 (95.14%), Query Frame = 0
Query: 246 DWQSCIWKQNVLCGSDRTYCKTLDQFGPGNVLRNRIIDAKLTGGSRVFYKVPKKRNRIVA 305
D WKQNVL GS+RTYCKTLDQFGP +VLRNRI DAKLTGGSRV YKVPKKRNRIVA
Sbjct: 2 DLSCSTWKQNVLRGSERTYCKTLDQFGPRSVLRNRIFDAKLTGGSRVSYKVPKKRNRIVA 61
Query: 306 SINSNHLSLVCASKFDGALQIFNHKRFLNFNNKIIGRRTRMVNSECQSNDSLAFIDGNGR 365
S +SNHLSLVCASKFD ALQIF KRFLN+NNKI GR MV+ ECQ+NDSLAFIDGNGR
Sbjct: 62 SSDSNHLSLVCASKFDRALQIFTPKRFLNWNNKISGRGMGMVHLECQNNDSLAFIDGNGR 121
Query: 366 NVEYVDTSDEGSSSEPTDGVGSAGSKEAGGEAEAVETDAPTVDELRELLQKAMTELEVAR 425
N+EYV++ DEGSSS P DGVGSAGS+E GGEAE VET+ PTVDELRELLQKAM ELEVAR
Sbjct: 122 NIEYVNSGDEGSSSGPADGVGSAGSREVGGEAETVETNIPTVDELRELLQKAMKELEVAR 181
Query: 426 LNSTMFEEKAQKISETAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMALS 485
LNSTMFEE+AQKISE AIALQDEA +AWNDVNSTLDSVQQIVNEEY AKEAVQKATMALS
Sbjct: 182 LNSTMFEERAQKISEAAIALQDEATNAWNDVNSTLDSVQQIVNEEYVAKEAVQKATMALS 241
Query: 486 LAEARLQVATESLDLAKGGNDSPETSIDSEGEIDGKEEQEVLLVAQEDIKECRANLEICD 545
LAEARLQVA ESL+LAK G+D PETS+DS+G IDGKE+QE LLVAQEDI +CRANLEIC+
Sbjct: 242 LAEARLQVAIESLELAKRGSDFPETSMDSDGVIDGKEDQEALLVAQEDITDCRANLEICN 301
Query: 546 AELKRVQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVS 605
AEL R+QSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRV+
Sbjct: 302 AELMRLQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVN 361
Query: 606 DAEIVLQKVEKSLSNSFVDTSETTQGSNVIEEVVNEDNKAVLEISGDIAVEMDRDLPLSG 665
DAE LQKVEKSLS+SFVDTS+ TQGSNVIEEV NEDNKAVLEISGDIAVEMDR+LPL+G
Sbjct: 362 DAERALQKVEKSLSSSFVDTSDITQGSNVIEEVENEDNKAVLEISGDIAVEMDRELPLNG 421
Query: 666 DSLAVKSLPGSFSDSEDSDQAYDLSDNDNGKLSSDSFKEVEAGAEKSVLSQAKKQETQKD 725
DSLA+KSLPGS SDSE SDQ Y LSD++NGKLSSDS KEVE+GAEKS+LSQ KKQE QKD
Sbjct: 422 DSLAIKSLPGSLSDSEGSDQPYYLSDSENGKLSSDSAKEVESGAEKSILSQTKKQEIQKD 481
Query: 726 LTREGSPLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALV----LDFTKKQLPKLVVGV 785
LTREGSPLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALV LD TKKQLPKL+VG
Sbjct: 482 LTREGSPLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALVFQGLLDSTKKQLPKLIVGA 541
Query: 786 VLLGAGITIFSNRAERSSQMFQQPDAVSISTDEVSLNTKPLFQQLRKLPKRVKKLIAKIP 845
VLLGAGI +F+NRAERSSQM QPD V+ISTD+VSL+TKPLFQQLRKLPKRVKKLI++IP
Sbjct: 542 VLLGAGIAVFANRAERSSQMIPQPDVVTISTDDVSLDTKPLFQQLRKLPKRVKKLISQIP 601
Query: 846 HQEVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHG 905
HQEVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHG
Sbjct: 602 HQEVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHG 661
Query: 906 TKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPGP 965
TKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAH+VCGQ GP
Sbjct: 662 TKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHMVCGQAGP 721
Query: 966 AAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNS 1025
AAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNS
Sbjct: 722 AAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNS 781
Query: 1026 SKGGIGFQAIAEALGVAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVI 1085
SKGGIGFQAIAEALG+AAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVI
Sbjct: 782 SKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVI 841
Query: 1086 LGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPK 1145
LGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPK
Sbjct: 842 LGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPK 901
Query: 1146 LLLSNFPVITGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEAV 1205
LL SNFPVI GSLGLLIGGKTILVALVGRLFGISIISA+RVGLLLAPGGEFAFVAFGEAV
Sbjct: 902 LLFSNFPVIMGSLGLLIGGKTILVALVGRLFGISIISAIRVGLLLAPGGEFAFVAFGEAV 961
Query: 1206 NQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHI 1265
NQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHI
Sbjct: 962 NQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHI 1021
Query: 1266 IICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAE 1325
IICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAE
Sbjct: 1022 IICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAE 1081
Query: 1326 RACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPS 1385
RACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPS
Sbjct: 1082 RACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPS 1141
Query: 1386 LQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTS 1445
LQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTS
Sbjct: 1142 LQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTS 1201
Query: 1446 DSSDENQITEGTLAI 1457
DSSDENQ+TEGTLAI
Sbjct: 1202 DSSDENQVTEGTLAI 1216
BLAST of Lag0020772 vs. ExPASy TrEMBL
Match:
A0A1S3AW31 (K(+) efflux antiporter 2, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103483290 PE=4 SV=1)
HSP 1 Score: 2040.8 bits (5286), Expect = 0.0e+00
Identity = 1113/1215 (91.60%), Postives = 1156/1215 (95.14%), Query Frame = 0
Query: 246 DWQSCIWKQNVLCGSDRTYCKTLDQFGPGNVLRNRIIDAKLTGGSRVFYKVPKKRNRIVA 305
D WKQNVL GS+RTYCKTLDQFGP +VLRNRI DAKLTGGSRV YKVPKKRNRIVA
Sbjct: 2 DLSCSTWKQNVLRGSERTYCKTLDQFGPRSVLRNRIFDAKLTGGSRVSYKVPKKRNRIVA 61
Query: 306 SINSNHLSLVCASKFDGALQIFNHKRFLNFNNKIIGRRTRMVNSECQSNDSLAFIDGNGR 365
S +SNHLSLVCASKFD ALQIF KRFLN+NNKI GR MV+ ECQ+NDSLAFIDGNGR
Sbjct: 62 SSDSNHLSLVCASKFDRALQIFTPKRFLNWNNKISGRGMGMVHLECQNNDSLAFIDGNGR 121
Query: 366 NVEYVDTSDEGSSSEPTDGVGSAGSKEAGGEAEAVETDAPTVDELRELLQKAMTELEVAR 425
N+EYV++ DEGSSS P DGVGSAGS+E GGEAE VET+ PTVDELRELLQKAM ELEVAR
Sbjct: 122 NIEYVNSGDEGSSSGPADGVGSAGSREVGGEAETVETNIPTVDELRELLQKAMKELEVAR 181
Query: 426 LNSTMFEEKAQKISETAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMALS 485
LNSTMFEE+AQKISE AIALQDEA +AWNDVNSTLDSVQQIVNEEY AKEAVQKATMALS
Sbjct: 182 LNSTMFEERAQKISEAAIALQDEATNAWNDVNSTLDSVQQIVNEEYVAKEAVQKATMALS 241
Query: 486 LAEARLQVATESLDLAKGGNDSPETSIDSEGEIDGKEEQEVLLVAQEDIKECRANLEICD 545
LAEARLQVA ESL+LAK G+D PETS+DS+G IDGKE+QE LLVAQEDI +CRANLEIC+
Sbjct: 242 LAEARLQVAIESLELAKRGSDFPETSMDSDGVIDGKEDQEALLVAQEDITDCRANLEICN 301
Query: 546 AELKRVQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVS 605
AEL R+QSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRV+
Sbjct: 302 AELMRLQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVN 361
Query: 606 DAEIVLQKVEKSLSNSFVDTSETTQGSNVIEEVVNEDNKAVLEISGDIAVEMDRDLPLSG 665
DAE LQKVEKSLS+SFVDTS+ TQGSNVIEEV NEDNKAVLEISGDIAVEMDR+LPL+G
Sbjct: 362 DAERALQKVEKSLSSSFVDTSDITQGSNVIEEVENEDNKAVLEISGDIAVEMDRELPLNG 421
Query: 666 DSLAVKSLPGSFSDSEDSDQAYDLSDNDNGKLSSDSFKEVEAGAEKSVLSQAKKQETQKD 725
DSLA+KSLPGS SDSE SDQ Y LSD++NGKLSSDS KEVE+GAEKS+LSQ KKQE QKD
Sbjct: 422 DSLAIKSLPGSLSDSEGSDQPYYLSDSENGKLSSDSAKEVESGAEKSILSQTKKQEIQKD 481
Query: 726 LTREGSPLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALV----LDFTKKQLPKLVVGV 785
LTREGSPLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALV LD TKKQLPKL+VG
Sbjct: 482 LTREGSPLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALVFQGLLDSTKKQLPKLIVGA 541
Query: 786 VLLGAGITIFSNRAERSSQMFQQPDAVSISTDEVSLNTKPLFQQLRKLPKRVKKLIAKIP 845
VLLGAGI +F+NRAERSSQM QPD V+ISTD+VSL+TKPLFQQLRKLPKRVKKLI++IP
Sbjct: 542 VLLGAGIAVFANRAERSSQMIPQPDVVTISTDDVSLDTKPLFQQLRKLPKRVKKLISQIP 601
Query: 846 HQEVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHG 905
HQEVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHG
Sbjct: 602 HQEVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHG 661
Query: 906 TKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPGP 965
TKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAH+VCGQ GP
Sbjct: 662 TKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHMVCGQAGP 721
Query: 966 AAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNS 1025
AAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNS
Sbjct: 722 AAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNS 781
Query: 1026 SKGGIGFQAIAEALGVAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVI 1085
SKGGIGFQAIAEALG+AAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVI
Sbjct: 782 SKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVI 841
Query: 1086 LGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPK 1145
LGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPK
Sbjct: 842 LGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPK 901
Query: 1146 LLLSNFPVITGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEAV 1205
LL SNFPVI GSLGLLIGGKTILVALVGRLFGISIISA+RVGLLLAPGGEFAFVAFGEAV
Sbjct: 902 LLFSNFPVIMGSLGLLIGGKTILVALVGRLFGISIISAIRVGLLLAPGGEFAFVAFGEAV 961
Query: 1206 NQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHI 1265
NQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHI
Sbjct: 962 NQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHI 1021
Query: 1266 IICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAE 1325
IICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAE
Sbjct: 1022 IICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAE 1081
Query: 1326 RACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPS 1385
RACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPS
Sbjct: 1082 RACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPS 1141
Query: 1386 LQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTS 1445
LQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTS
Sbjct: 1142 LQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTS 1201
Query: 1446 DSSDENQITEGTLAI 1457
DSSDENQ+TEGTLAI
Sbjct: 1202 DSSDENQVTEGTLAI 1216
BLAST of Lag0020772 vs. TAIR 10
Match:
AT4G00630.1 (K+ efflux antiporter 2 )
HSP 1 Score: 1442.9 bits (3734), Expect = 0.0e+00
Identity = 858/1225 (70.04%), Postives = 975/1225 (79.59%), Query Frame = 0
Query: 246 DWQSCIWKQNVLCGSD--RTYCKTLDQFGPGNVLRNRIIDAKL----TGGSRVFYKV--P 305
D+ S + +Q++ G +YC L NR+I AKL GG+R + +
Sbjct: 2 DFASSVQRQSMFHGGADFASYC-----------LPNRMISAKLCPKGLGGTRFWDPMIDS 61
Query: 306 KKRNRIVASIN-SNHLSLVCASKFDGALQIFNHKRFLNFNNKIIGRRTRMVNSECQSNDS 365
K R+ I + N S SL + F+G + + H N +G R CQS+DS
Sbjct: 62 KVRSAIRSKRNVSYRSSLTLNADFNG--RFYGHLLPAKPQNVPLGFRLL-----CQSSDS 121
Query: 366 LAFIDGNGRNVEYVDTSDEGSSSEPTDGVGSAGSKEAGGEAEAVETDAPTVDELRELLQK 425
+ + GN RN+E+ + SD+ + SKE E + AP+++ELR+LL K
Sbjct: 122 VGDLVGNDRNLEFAEGSDDREVT---------FSKEEKDTRE--QDSAPSLEELRDLLNK 181
Query: 426 AMTELEVARLNSTMFEEKAQKISETAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEA 485
A ELEVA LNSTMFEEKAQ+ISE AIAL+DEAA AWNDVN TL+ VQ+ V+EE AKEA
Sbjct: 182 ATKELEVASLNSTMFEEKAQRISEVAIALKDEAASAWNDVNQTLNVVQEAVDEESVAKEA 241
Query: 486 VQKATMALSLAEARLQVATESLDLAKGGNDSPETSIDSEGEIDG-KEEQEVLLVAQEDIK 545
VQKATMALSLAEARLQVA ESL+ A+G N S E+ + DG K+++E LL A+ DIK
Sbjct: 242 VQKATMALSLAEARLQVALESLE-AEGYNTSEESEVR-----DGVKDKEEALLSAKADIK 301
Query: 546 ECRANLEICDAELKRVQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVA 605
EC+ NL C+ +L+R+Q KK+ELQKEVDRLNE AE+AQ++ALKAEEDVANIM+LAEQAVA
Sbjct: 302 ECQENLASCEEQLRRLQVKKDELQKEVDRLNEAAERAQISALKAEEDVANIMVLAEQAVA 361
Query: 606 FELEAAQRVSDAEIVLQKVEKSLSNSFVDTSETTQGS--NVIEEVVNEDNKAVLEISGDI 665
FELEA QRV+DAEI LQ+ EK+L S T ETTQG + +V ED VL D+
Sbjct: 362 FELEATQRVNDAEIALQRAEKTLFGS--QTQETTQGKVLDGKNTIVGEDE--VLSEIVDV 421
Query: 666 AVEMDRDLPLSGDSLAVKSLPGSFSDSEDSDQAYDLSDNDNGKLSSDSFKEVEAGAEKSV 725
+ + +RDL + G S S+ Q+Y+ SDN+NGK ++D KE E AEKS
Sbjct: 422 SHQAERDLVVVGVS------------SDVGTQSYE-SDNENGKPTADFAKEAEGEAEKSK 481
Query: 726 -LSQAKKQETQKDLTREGSPLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALVLDFTKK 785
+ KKQE QKDL RE S N K LKKSSRFF ASFFS DGT +++ K+
Sbjct: 482 NVVLTKKQEVQKDLPRESSSHNGTKTSLKKSSRFFPASFFSSNGDGTATVFESLVESAKQ 541
Query: 786 QLPKLVVGVVLLGAGITIFSNRAERSSQMFQQPDAVSISTDEVSLNTKPLFQQLRKLPKR 845
Q PKL++G LLGAG+ I+SN R++Q+ QQP+ VS S ++VS +TKPL +Q++KLPKR
Sbjct: 542 QWPKLILGFTLLGAGVAIYSNGVGRNNQLPQQPNIVSTSAEDVSSSTKPLIRQMQKLPKR 601
Query: 846 VKKLIAKIPHQEVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYG 905
+KKL+ P QEVNEEEASLLD+LWLLLASVIFVP FQK+PGGSPVLGYLAAGILIGPYG
Sbjct: 602 IKKLLEMFPQQEVNEEEASLLDVLWLLLASVIFVPLFQKIPGGSPVLGYLAAGILIGPYG 661
Query: 906 LSIIRHVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVA 965
LSIIR+VHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTA V+GL+
Sbjct: 662 LSIIRNVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAAVIGLIT 721
Query: 966 HIVCGQPGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLI 1025
H V GQ GPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLI
Sbjct: 722 HYVAGQAGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLI 781
Query: 1026 LIPLISPNSSKGGIGFQAIAEALGVAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEI 1085
LIPLISPNSSKGGIGFQAIAEALG+AA+KA VAI+ IIAGGRLLLRPIYKQIAEN+NAEI
Sbjct: 782 LIPLISPNSSKGGIGFQAIAEALGLAAIKAAVAITGIIAGGRLLLRPIYKQIAENRNAEI 841
Query: 1086 FSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFM 1145
FSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFM
Sbjct: 842 FSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFM 901
Query: 1146 TVGMSIDPKLLLSNFPVITGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEF 1205
TVGMSIDPKLLL+NFP+I G+LGLL+ GKTILV ++G+LFGISIISA+RVGLLLAPGGEF
Sbjct: 902 TVGMSIDPKLLLANFPLIMGTLGLLLVGKTILVVIIGKLFGISIISAVRVGLLLAPGGEF 961
Query: 1206 AFVAFGEAVNQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVES 1265
AFVAFGEAVNQGIM+ QLSSLLFLVVGISMALTPWLAAGGQLIASRFE DVRSLLPVES
Sbjct: 962 AFVAFGEAVNQGIMTPQLSSLLFLVVGISMALTPWLAAGGQLIASRFELQDVRSLLPVES 1021
Query: 1266 ETDDLQDHIIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSR 1325
ETDDLQ HIIICGFGR+GQIIAQLLSERLIPFVALDV SDRVA+GR+LDLPVYFGDAGSR
Sbjct: 1022 ETDDLQGHIIICGFGRIGQIIAQLLSERLIPFVALDVSSDRVAIGRSLDLPVYFGDAGSR 1081
Query: 1326 EVLHKVGAERACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATA 1385
EVLHK+GA+RACAAAI LDTPGANYR VWALSKYFPNVKTFVRAHDVDHGLNLEKAGATA
Sbjct: 1082 EVLHKIGADRACAAAIALDTPGANYRCVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATA 1141
Query: 1386 VVPETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRI 1445
VVPETLEPSLQLAAAVLAQAKLP SEIA TINEFRSRHLSEL ELCEASGSSLGYGFSR
Sbjct: 1142 VVPETLEPSLQLAAAVLAQAKLPTSEIATTINEFRSRHLSELAELCEASGSSLGYGFSRS 1174
Query: 1446 MSKPKIQT-SDSSDENQITEGTLAI 1457
SKPK + S++SD+NQI EGTLAI
Sbjct: 1202 TSKPKPPSPSETSDDNQIIEGTLAI 1174
BLAST of Lag0020772 vs. TAIR 10
Match:
AT1G01790.1 (K+ efflux antiporter 1 )
HSP 1 Score: 1436.8 bits (3718), Expect = 0.0e+00
Identity = 833/1183 (70.41%), Postives = 943/1183 (79.71%), Query Frame = 0
Query: 279 NRIIDAK---LTGGSRVFYKVPKKRNRIVASINSNHLSLVCASKFDGALQIFNHKRFLNF 338
NR+I K +T G + + R + S N ++ + +F + + K L
Sbjct: 25 NRLISPKGISITSGDSKVHSCFRLRRNVAQSGTLNLMNACFSGRFYSG-HLHSTKSILG- 84
Query: 339 NNKIIGRRTRMVNSECQSNDSLAFIDGNGRNVEYVDTSDEGSSSEPTDGVGSAGSKEAGG 398
N R CQ ++SL D N + G SSE +D + K+A
Sbjct: 85 NGHQAKRIPFGFRLRCQGHESLGNADSNDHRI--------GESSESSDETEATDLKDA-- 144
Query: 399 EAEAVETDAPTVDELRELLQKAMTELEVARLNSTMFEEKAQKISETAIALQDEAAHAWND 458
VE D +++EL+ELL KA+ ELEVARLNSTMFEEKAQ+ISE AIAL+DEAA AW D
Sbjct: 145 ---RVENDTDSLEELKELLHKAIKELEVARLNSTMFEEKAQRISERAIALKDEAATAWLD 204
Query: 459 VNSTLDSVQQIVNEEYAAKEAVQKATMALSLAEARLQVATESLDLAKGGNDSPETSIDSE 518
VN TLD ++ V EE AKEAVQ ATMALSLAEARLQV ESL+ A GND P S ++E
Sbjct: 205 VNKTLDVIRDTVYEEALAKEAVQTATMALSLAEARLQVIVESLE-AGAGNDIPHVSEETE 264
Query: 519 GEIDGKEEQEVLLVAQEDIKECRANLEICDAELKRVQSKKEELQKEVDRLNELAEKAQLN 578
ID +++E LL A++DIKEC+ NL+ C+++L + SKK+ELQKEVD+LNE AE Q++
Sbjct: 265 ETIDVNDKEEALLAAKDDIKECQVNLDNCESQLSALLSKKDELQKEVDKLNEFAETIQIS 324
Query: 579 ALKAEEDVANIMLLAEQAVAFELEAAQRVSDAEIVLQKVEKSLSNSFVDTSETTQGSNVI 638
+LKAEEDV NIM LAEQAVAFELEA QRV+DAEI LQ+ EKSLS S T E TQG
Sbjct: 325 SLKAEEDVTNIMKLAEQAVAFELEATQRVNDAEIALQRAEKSLSIS--QTPEETQGQLSD 384
Query: 639 EEVVNEDNKAVLEISGDIAVEMDRDLPLSGDSLAVKSLPGSFSD-----SEDSDQAYDLS 698
EE ED + D+ +++++ P GD V+ D ++ Q Y+ S
Sbjct: 385 EETSQEDAMVLSGNVEDVTHQVEKESPKDGDLPVVQITAELVPDIVGQRNKKLTQPYESS 444
Query: 699 DNDNGKLSSDSFKEVEAGAEKSVLS-QAKKQETQKDLTREGSPLNSPKALLKKSSRFFSA 758
D++NGK S +S K VEA +EK ++ Q KKQETQKDL +EGS LNSPKA KSSRFFSA
Sbjct: 445 DHENGKPSVESSKVVEADSEKPKINVQTKKQETQKDLPKEGSSLNSPKASFNKSSRFFSA 504
Query: 759 SFFSFTVDGTEFTPALVLDFTKKQLPKLVVGVVLLGAGITIFSNRAERSSQMFQQPDAVS 818
SFFS DGT ++ K+Q PKLV+G+ LLGAG+T++SN ++Q+ QQPD S
Sbjct: 505 SFFSSNPDGTATVFGSLVGSVKQQWPKLVLGLALLGAGLTLYSNGVGGNNQLLQQPDVTS 564
Query: 819 ISTDEVSLNTKPLFQQLRKLPKRVKKLIAKIPHQEVNEEEASLLDMLWLLLASVIFVPTF 878
ST++VS +TKPL +Q++KLPKR+KKL+ IPHQEVNEEEASL D LWLLLASVIFVP F
Sbjct: 565 TSTEDVSSSTKPLIRQVQKLPKRIKKLLEMIPHQEVNEEEASLFDFLWLLLASVIFVPLF 624
Query: 879 QKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAEFGVVFLLFNIGLELSVERLSSM 938
QK+PGGSPVLGYLAAGILIGPYGLSIIR+VHGT+AIAEFGVVFLLFNIGLELSVERLSSM
Sbjct: 625 QKIPGGSPVLGYLAAGILIGPYGLSIIRNVHGTRAIAEFGVVFLLFNIGLELSVERLSSM 684
Query: 939 KKYVFGLGSAQVLVTAVVVGLVAHIVCGQPGPAAIVIGNGLALSSTAVVLQVLQERGEST 998
KKYVFGLGSAQVLVTA VVGL+AH V GQ GPAAIVIGNGLALSSTAVVLQVLQERGEST
Sbjct: 685 KKYVFGLGSAQVLVTAAVVGLLAHYVAGQAGPAAIVIGNGLALSSTAVVLQVLQERGEST 744
Query: 999 SRHGRATFSVLLFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGVAAVKAIVAISAI 1058
SRHGRA+FSVLLFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALG+AAVKA VAI+AI
Sbjct: 745 SRHGRASFSVLLFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAAVKAAVAITAI 804
Query: 1059 IAGGRLLLRPIYKQIAENQNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAET 1118
IAGGRLLLRPIYKQIAEN+NAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAET
Sbjct: 805 IAGGRLLLRPIYKQIAENRNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAET 864
Query: 1119 EFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNFPVITGSLGLLIGGKTILVALVG 1178
EFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNFPVI G+LGLLI GKT+LV ++G
Sbjct: 865 EFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNFPVIVGTLGLLIVGKTMLVVIMG 924
Query: 1179 RLFGISIISALRVGLLLAPGGEFAFVAFGEAVNQGIMSSQLSSLLFLVVGISMALTPWLA 1238
+LFGISIISA+RVGLLLAPGGEFAFVAFGEAVNQGIMS QLSSLLFLVVGISMA+TPWLA
Sbjct: 925 KLFGISIISAIRVGLLLAPGGEFAFVAFGEAVNQGIMSPQLSSLLFLVVGISMAITPWLA 984
Query: 1239 AGGQLIASRFEQHDVRSLLPVESETDDLQDHIIICGFGRVGQIIAQLLSERLIPFVALDV 1298
AGGQLIASRFE HDVRSLLPVESETDDLQ HIIICGFGRVGQIIAQLLSERLIPFVALDV
Sbjct: 985 AGGQLIASRFELHDVRSLLPVESETDDLQGHIIICGFGRVGQIIAQLLSERLIPFVALDV 1044
Query: 1299 RSDRVAVGRALDLPVYFGDAGSREVLHKVGAERACAAAITLDTPGANYRTVWALSKYFPN 1358
SDRV +GR+LDLPVYFGDAGS+EVLHK+GA RACAA + LD PGANYR VWALSK++PN
Sbjct: 1045 SSDRVTIGRSLDLPVYFGDAGSKEVLHKIGAGRACAAVVALDAPGANYRCVWALSKFYPN 1104
Query: 1359 VKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSR 1418
VKTFVRAHDV HGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLP SEIA TINEFR+R
Sbjct: 1105 VKTFVRAHDVVHGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPTSEIANTINEFRTR 1164
Query: 1419 HLSELTELCEASGSSLGYGFSRIMSKPKIQTSDSSDENQITEG 1453
HLSELTELCEASGSSLGYG+SR SKPK Q SD+S +NQI EG
Sbjct: 1165 HLSELTELCEASGSSLGYGYSR-TSKPKPQPSDASGDNQIIEG 1188
BLAST of Lag0020772 vs. TAIR 10
Match:
AT4G00630.2 (K+ efflux antiporter 2 )
HSP 1 Score: 1434.5 bits (3712), Expect = 0.0e+00
Identity = 858/1236 (69.42%), Postives = 975/1236 (78.88%), Query Frame = 0
Query: 246 DWQSCIWKQNVLCGSD--RTYCKTLDQFGPGNVLRNRIIDAKL----TGGSRVFYKV--P 305
D+ S + +Q++ G +YC L NR+I AKL GG+R + +
Sbjct: 2 DFASSVQRQSMFHGGADFASYC-----------LPNRMISAKLCPKGLGGTRFWDPMIDS 61
Query: 306 KKRNRIVASIN-SNHLSLVCASKFDGALQIFNHKRFLNFNNKIIGRRTRMVNSECQSNDS 365
K R+ I + N S SL + F+G + + H N +G R CQS+DS
Sbjct: 62 KVRSAIRSKRNVSYRSSLTLNADFNG--RFYGHLLPAKPQNVPLGFRLL-----CQSSDS 121
Query: 366 LAFIDGNGRNVEYVDTSDEGSSSEPTDGVGSAGSKEAGGEAEAVETDAPTVDELRELLQK 425
+ + GN RN+E+ + SD+ + SKE E + AP+++ELR+LL K
Sbjct: 122 VGDLVGNDRNLEFAEGSDDREVT---------FSKEEKDTRE--QDSAPSLEELRDLLNK 181
Query: 426 AMTELEVARLNSTMFEEKAQKISETAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEA 485
A ELEVA LNSTMFEEKAQ+ISE AIAL+DEAA AWNDVN TL+ VQ+ V+EE AKEA
Sbjct: 182 ATKELEVASLNSTMFEEKAQRISEVAIALKDEAASAWNDVNQTLNVVQEAVDEESVAKEA 241
Query: 486 VQKATMALSLAEARLQVATESLDLAKGGNDSPETSIDSEGEIDG-KEEQEVLLVAQEDIK 545
VQKATMALSLAEARLQVA ESL+ A+G N S E+ + DG K+++E LL A+ DIK
Sbjct: 242 VQKATMALSLAEARLQVALESLE-AEGYNTSEESEVR-----DGVKDKEEALLSAKADIK 301
Query: 546 ECRANLEICDAELKRVQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVA 605
EC+ NL C+ +L+R+Q KK+ELQKEVDRLNE AE+AQ++ALKAEEDVANIM+LAEQAVA
Sbjct: 302 ECQENLASCEEQLRRLQVKKDELQKEVDRLNEAAERAQISALKAEEDVANIMVLAEQAVA 361
Query: 606 FELEAAQRVSDAEIVLQKVEKSLSNSFVDTSETTQGS--NVIEEVVNEDNKAVLEISGDI 665
FELEA QRV+DAEI LQ+ EK+L S T ETTQG + +V ED VL D+
Sbjct: 362 FELEATQRVNDAEIALQRAEKTLFGS--QTQETTQGKVLDGKNTIVGEDE--VLSEIVDV 421
Query: 666 AVEMDRDLPLSGDSLAVKSLPGSFSDSEDSDQAYDLSDNDNGKLSSDSFKEVEAGAEKSV 725
+ + +RDL + G S S+ Q+Y+ SDN+NGK ++D KE E AEKS
Sbjct: 422 SHQAERDLVVVGVS------------SDVGTQSYE-SDNENGKPTADFAKEAEGEAEKSK 481
Query: 726 -LSQAKKQETQKDLTREGSPLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALVLDFTKK 785
+ KKQE QKDL RE S N K LKKSSRFF ASFFS DGT +++ K+
Sbjct: 482 NVVLTKKQEVQKDLPRESSSHNGTKTSLKKSSRFFPASFFSSNGDGTATVFESLVESAKQ 541
Query: 786 QLPKLVVGVVLLGAGITIFSNRAERSSQMFQQPDAVSISTDEVSLNTKPLFQQLRKLPKR 845
Q PKL++G LLGAG+ I+SN R++Q+ QQP+ VS S ++VS +TKPL +Q++KLPKR
Sbjct: 542 QWPKLILGFTLLGAGVAIYSNGVGRNNQLPQQPNIVSTSAEDVSSSTKPLIRQMQKLPKR 601
Query: 846 VKKLIAKIPHQEVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYG 905
+KKL+ P QEVNEEEASLLD+LWLLLASVIFVP FQK+PGGSPVLGYLAAGILIGPYG
Sbjct: 602 IKKLLEMFPQQEVNEEEASLLDVLWLLLASVIFVPLFQKIPGGSPVLGYLAAGILIGPYG 661
Query: 906 LSIIRHVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVA 965
LSIIR+VHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTA V+GL+
Sbjct: 662 LSIIRNVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAAVIGLIT 721
Query: 966 HIVCGQPGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLI 1025
H V GQ GPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLI
Sbjct: 722 HYVAGQAGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLI 781
Query: 1026 LIPLISPNSSKGGIGFQAIAEALGVAAVKAIVAISAIIAGGRL-----------LLRPIY 1085
LIPLISPNSSKGGIGFQAIAEALG+AA+KA VAI+ IIAGGRL LLRPIY
Sbjct: 782 LIPLISPNSSKGGIGFQAIAEALGLAAIKAAVAITGIIAGGRLFYNALIFWYTQLLRPIY 841
Query: 1086 KQIAENQNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAP 1145
KQIAEN+NAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAP
Sbjct: 842 KQIAENRNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAP 901
Query: 1146 YRGLLLGLFFMTVGMSIDPKLLLSNFPVITGSLGLLIGGKTILVALVGRLFGISIISALR 1205
YRGLLLGLFFMTVGMSIDPKLLL+NFP+I G+LGLL+ GKTILV ++G+LFGISIISA+R
Sbjct: 902 YRGLLLGLFFMTVGMSIDPKLLLANFPLIMGTLGLLLVGKTILVVIIGKLFGISIISAVR 961
Query: 1206 VGLLLAPGGEFAFVAFGEAVNQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQ 1265
VGLLLAPGGEFAFVAFGEAVNQGIM+ QLSSLLFLVVGISMALTPWLAAGGQLIASRFE
Sbjct: 962 VGLLLAPGGEFAFVAFGEAVNQGIMTPQLSSLLFLVVGISMALTPWLAAGGQLIASRFEL 1021
Query: 1266 HDVRSLLPVESETDDLQDHIIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALD 1325
DVRSLLPVESETDDLQ HIIICGFGR+GQIIAQLLSERLIPFVALDV SDRVA+GR+LD
Sbjct: 1022 QDVRSLLPVESETDDLQGHIIICGFGRIGQIIAQLLSERLIPFVALDVSSDRVAIGRSLD 1081
Query: 1326 LPVYFGDAGSREVLHKVGAERACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDH 1385
LPVYFGDAGSREVLHK+GA+RACAAAI LDTPGANYR VWALSKYFPNVKTFVRAHDVDH
Sbjct: 1082 LPVYFGDAGSREVLHKIGADRACAAAIALDTPGANYRCVWALSKYFPNVKTFVRAHDVDH 1141
Query: 1386 GLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEAS 1445
GLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLP SEIA TINEFRSRHLSEL ELCEAS
Sbjct: 1142 GLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPTSEIATTINEFRSRHLSELAELCEAS 1185
Query: 1446 GSSLGYGFSRIMSKPKIQT-SDSSDENQITEGTLAI 1457
GSSLGYGFSR SKPK + S++SD+NQI EGTLAI
Sbjct: 1202 GSSLGYGFSRSTSKPKPPSPSETSDDNQIIEGTLAI 1185
BLAST of Lag0020772 vs. TAIR 10
Match:
AT4G04850.2 (K+ efflux antiporter 3 )
HSP 1 Score: 335.5 bits (859), Expect = 2.1e-91
Identity = 223/601 (37.10%), Postives = 333/601 (55.41%), Query Frame = 0
Query: 854 DMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAEFGVVFL 913
D L L+ +VI VP F+ L SP+LG+ AG+++ +GL IR++ K ++E+G++FL
Sbjct: 107 DTLTFLMVTVIIVPAFRILK-ASPILGFFFAGVVLNQFGL--IRNLTDVKVLSEWGILFL 166
Query: 914 LFNIGLELSVERLSSMKKYVFGLGSAQVLVT-------------AVVVGLVAHIVCGQPG 973
LF +GLELS+ RL ++ K+ FG+G QVL+ A+ ++ + +P
Sbjct: 167 LFEMGLELSLARLKALAKFAFGMGLTQVLLCTAAFTAFELPPNGAIGTKILEFLFHSRPD 226
Query: 974 -------PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLIL 1033
A+VIG L+LSS+A VLQ+L E+GE +R G AT +LL QD+AVV LL++
Sbjct: 227 LVNIRSIDEAVVIGAALSLSSSAFVLQLLAEKGELPTRFGSATLGILLLQDIAVVPLLVI 286
Query: 1034 IPLISPNSSKGGIGFQAIAEALGVAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIF 1093
+P++ IG ++I L + KA+ + + GG+ LR I++ +AE +++E F
Sbjct: 287 LPVLESQD----IGGESIWPMLAKESAKALGGLGILSLGGKFFLRRIFEVVAETRSSEAF 346
Query: 1094 SANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMT 1153
A LL + GTSL+T G S LGAFLAG LLAET F Q+E+DI P+RGLLLGLFF+T
Sbjct: 347 VALCLLTVAGTSLVTQWLGFSDTLGAFLAGALLAETNFRTQIEADIRPFRGLLLGLFFVT 406
Query: 1154 VGMSIDPKLLLSNFPVITGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFA 1213
G SID ++L +P + LG LI KT+++ +G G++I ++RVG LL+ GGEFA
Sbjct: 407 TGTSIDMEVLFREWPNVLSLLGGLIVIKTLIITAIGPRVGLTIQESVRVGFLLSQGGEFA 466
Query: 1214 FVAFGEAVNQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESE 1273
FV F A G++ ++L+ LL +VV +SMALTP+L G+ A ++ E
Sbjct: 467 FVVFSLANRLGVLPNELNKLLIIVVVLSMALTPYLNQLGRKAADFLDERLDPGEKIGEDV 526
Query: 1274 TDDLQDHIIICGFGRVGQIIAQLLSERLI--------PFVALDVRSDRVAVGRALDLPVY 1333
D+ + I+I GFG++GQ++A LS L+ P++ D+ V R L P+
Sbjct: 527 NFDVSESIVIIGFGQMGQVLANFLSTPLVSDSDLVGWPYIGFDLNPAVVKESRKLGFPIL 586
Query: 1334 FGDAGSREVLHKVGAERACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNL 1393
+GD VL G A I V L FP + RA D+ H L L
Sbjct: 587 YGDGSRPSVLQSAGVSSPKAIMIMYKGKKRTTEAVQRLRLAFPGSPIYARAQDLPHLLEL 646
Query: 1394 EKAGATAVVPETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSL 1427
+KAGAT + E E SLQL + +L + +++ FR + E AS ++
Sbjct: 647 KKAGATDAILENAETSLQLGSKLLTGFGVMSDDVSFLSKVFRDSMEIQAQEEITASETNA 700
BLAST of Lag0020772 vs. TAIR 10
Match:
AT4G04850.1 (K+ efflux antiporter 3 )
HSP 1 Score: 307.0 bits (785), Expect = 8.1e-83
Identity = 200/525 (38.10%), Postives = 297/525 (56.57%), Query Frame = 0
Query: 854 DMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAEFGVVFL 913
D L L+ +VI VP F+ L SP+LG+ AG+++ +GL IR++ K ++E+G++FL
Sbjct: 107 DTLTFLMVTVIIVPAFRILK-ASPILGFFFAGVVLNQFGL--IRNLTDVKVLSEWGILFL 166
Query: 914 LFNIGLELSVERLSSMKKYVFGLGSAQVLVT-------------AVVVGLVAHIVCGQPG 973
LF +GLELS+ RL ++ K+ FG+G QVL+ A+ ++ + +P
Sbjct: 167 LFEMGLELSLARLKALAKFAFGMGLTQVLLCTAAFTAFELPPNGAIGTKILEFLFHSRPD 226
Query: 974 -------PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLIL 1033
A+VIG L+LSS+A VLQ+L E+GE +R G AT +LL QD+AVV LL++
Sbjct: 227 LVNIRSIDEAVVIGAALSLSSSAFVLQLLAEKGELPTRFGSATLGILLLQDIAVVPLLVI 286
Query: 1034 IPLISPNSSKGGIGFQAIAEALGVAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIF 1093
+P++ IG ++I L + KA+ + + GG+ LR I++ +AE +++E F
Sbjct: 287 LPVLESQD----IGGESIWPMLAKESAKALGGLGILSLGGKFFLRRIFEVVAETRSSEAF 346
Query: 1094 SANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMT 1153
A LL + GTSL+T G S LGAFLAG LLAET F Q+E+DI P+RGLLLGLFF+T
Sbjct: 347 VALCLLTVAGTSLVTQWLGFSDTLGAFLAGALLAETNFRTQIEADIRPFRGLLLGLFFVT 406
Query: 1154 VGMSIDPKLLLSNFPVITGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFA 1213
G SID ++L +P + LG LI KT+++ +G G++I ++RVG LL+ GGEFA
Sbjct: 407 TGTSIDMEVLFREWPNVLSLLGGLIVIKTLIITAIGPRVGLTIQESVRVGFLLSQGGEFA 466
Query: 1214 FVAFGEAVNQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESE 1273
FV F A G++ ++L+ LL +VV +SMALTP+L G+ A ++ E
Sbjct: 467 FVVFSLANRLGVLPNELNKLLIIVVVLSMALTPYLNQLGRKAADFLDERLDPGEKIGEDV 526
Query: 1274 TDDLQDHIIICGFGRVGQIIAQLLSERLI--------PFVALDVRSDRVAVGRALDLPVY 1333
D+ + I+I GFG++GQ++A LS L+ P++ D+ V R L P+
Sbjct: 527 NFDVSESIVIIGFGQMGQVLANFLSTPLVSDSDLVGWPYIGFDLNPAVVKESRKLGFPIL 586
Query: 1334 FGDAGSREVLHKVGAERACAAAITLDTPGANYRTVWALSKYFPNV 1351
+GD VL G A I V L FP V
Sbjct: 587 YGDGSRPSVLQSAGVSSPKAIMIMYKGKKRTTEAVQRLRLAFPGV 624
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038878214.1 | 0.0e+00 | 93.47 | K(+) efflux antiporter 2, chloroplastic [Benincasa hispida] | [more] |
KAG7028620.1 | 0.0e+00 | 88.61 | K(+) efflux antiporter 2, chloroplastic, partial [Cucurbita argyrosperma subsp. ... | [more] |
KAG7018984.1 | 0.0e+00 | 92.75 | K(+) efflux antiporter 2, chloroplastic, partial [Cucurbita argyrosperma subsp. ... | [more] |
KAA0048981.1 | 0.0e+00 | 89.70 | K(+) efflux antiporter 2 [Cucumis melo var. makuwa] | [more] |
XP_022980192.1 | 0.0e+00 | 92.58 | K(+) efflux antiporter 2, chloroplastic-like [Cucurbita maxima] >XP_022980193.1 ... | [more] |
Match Name | E-value | Identity | Description | |
O65272 | 0.0e+00 | 70.04 | K(+) efflux antiporter 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KEA2 ... | [more] |
Q9ZTZ7 | 0.0e+00 | 70.41 | K(+) efflux antiporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KEA1 ... | [more] |
Q9M0Z3 | 3.0e-90 | 37.10 | K(+) efflux antiporter 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KEA3 ... | [more] |
Q0ZAH7 | 5.7e-81 | 35.56 | Putative K(+) efflux antiporter KefB OS=Alkalimonas amylolytica OX=152573 GN=kef... | [more] |
A9MN27 | 5.7e-81 | 37.77 | Glutathione-regulated potassium-efflux system protein KefB OS=Salmonella arizona... | [more] |
Match Name | E-value | Identity | Description | |
A0A5A7U5W1 | 0.0e+00 | 89.70 | K(+) efflux antiporter 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... | [more] |
A0A6J1IYK3 | 0.0e+00 | 92.58 | K(+) efflux antiporter 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1... | [more] |
A0A6J1E9G2 | 0.0e+00 | 92.58 | K(+) efflux antiporter 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LO... | [more] |
A0A5D3D075 | 0.0e+00 | 91.60 | K(+) efflux antiporter 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... | [more] |
A0A1S3AW31 | 0.0e+00 | 91.60 | K(+) efflux antiporter 2, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC10348... | [more] |