Lag0020596 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0020596 vs. NCBI nr
Match: KAA0048836.1 (E3 ubiquitin-protein ligase BRE1-like 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1406.7 bits (3640), Expect = 0.0e+00
Identity = 802/970 (82.68%), Postives = 846/970 (87.22%), Query Frame = 0

Query: 1    MGSTVDSDRKRRHFSTISPTAATAKKTPFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE 60
            MGSTV+SDRKRRHFSTISPTAATAKK PFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE
Sbjct: 506  MGSTVESDRKRRHFSTISPTAATAKKAPFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE 565

Query: 61   FKSLQNKYAQLKEKQEPYDMTVAVVKNCWEELVNGLETSSVRMRRWRSKQDGEHTMAAAD 120
            +KSLQNKYAQLKEKQEPYD TVAVVKNCWEELVNGLETSSVRMRRWRSK+DGEHT+AA D
Sbjct: 566  YKSLQNKYAQLKEKQEPYDTTVAVVKNCWEELVNGLETSSVRMRRWRSKRDGEHTIAAVD 625

Query: 121  ESSSSFEDAVLSRLAETGATQSSSAYSSNEHMEEETESPSEKTKTIERNIEASIENLWYL 180
             SSSSFEDAVLSRLAETGATQSSSAYSS++HMEEETESP EKTKTIERNIE SIENLWYL
Sbjct: 626  GSSSSFEDAVLSRLAETGATQSSSAYSSSKHMEEETESPCEKTKTIERNIETSIENLWYL 685

Query: 181  KDGLHAALLNELPKDDSFRNRAFGDLVKEVRNMRLRVKDFLFKQKGLAIELQNHRDLDAK 240
            KDGLHA LL+ELPKDDSFRNR  GDLVKEVRNMRLRVKDFLFKQK LA EL+ HRDLDAK
Sbjct: 686  KDGLHATLLSELPKDDSFRNRTSGDLVKEVRNMRLRVKDFLFKQKVLAKELEKHRDLDAK 745

Query: 241  TKAELKVLKAELESAVAELEESNSKLTKLRAEHDAAKRDGFPVLNLSGKHSGGGKVRDKQ 300
            TKAELKVLK ELESAVAELEESNSKLTKLRAEHDAAK+ GFPVLNL+GKHS  GKVRDKQ
Sbjct: 746  TKAELKVLKVELESAVAELEESNSKLTKLRAEHDAAKKAGFPVLNLAGKHSASGKVRDKQ 805

Query: 301  KDLRDMESSLKELKDQAVGRLAELNSLHEGRLKMLQQLSDIQNTMKSVKSISSSQSYLLL 360
            KDLRDME SLKELKDQAV RLAELNSLHEGRLKML++LSDIQNTMKSVKSISSS+ YLLL
Sbjct: 806  KDLRDMEFSLKELKDQAVDRLAELNSLHEGRLKMLRRLSDIQNTMKSVKSISSSKPYLLL 865

Query: 361  RDRIEKLKLEVYEQQALFEKLQ-VEKDNIIWREKELNIKNNILDMLRRSSTVSDTRIDDL 420
            RDRIEKLK EV EQQALFEKLQ VEKDNIIW+EKELNIKNNILD+LRRSSTVSDTRI+DL
Sbjct: 866  RDRIEKLKSEVNEQQALFEKLQVVEKDNIIWKEKELNIKNNILDVLRRSSTVSDTRINDL 925

Query: 421  EMLIQKQKDEKKSMENKLAEVLKEPGRKKIISEFRALVSSFPEAMGSMQSQLHKYKEAAS 480
            E+LIQKQKD KKS+ENKL EVLKEPGRKKI+SEFRALVSSFPEAMGSMQSQLHKYKEAAS
Sbjct: 926  EILIQKQKDGKKSIENKLVEVLKEPGRKKIVSEFRALVSSFPEAMGSMQSQLHKYKEAAS 985

Query: 481  DVHSVRADMQSLSSIIDRMEKECESLSSRSKDQIAEIQKLQATVQDLTEVNRELKLIIDM 540
            DVHSVRADMQSLSSIIDRMEKECESLSSRSKDQ AEIQKLQATVQDLTEVNRELKLIIDM
Sbjct: 986  DVHSVRADMQSLSSIIDRMEKECESLSSRSKDQHAEIQKLQATVQDLTEVNRELKLIIDM 1045

Query: 541  YRRESTESREVLEARDLEYKAWAHVQSLKSSLDERNLESRVKIANEAEAISQQRLAAAEA 600
            Y RESTESREVLEARDLEYKAWA VQSLKSSLDERNLESRVK ANE+EAISQQRLAAAEA
Sbjct: 1046 YSRESTESREVLEARDLEYKAWARVQSLKSSLDERNLESRVKTANESEAISQQRLAAAEA 1105

Query: 601  EIARLRQKLEASKRDLTRLTDALKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITE 660
            EIARLRQKLEASKRDLTRL+D LKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITE
Sbjct: 1106 EIARLRQKLEASKRDLTRLSDVLKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITE 1165

Query: 661  RDDYNIKLVLEGVRARQMQEIMLIEKQTLEKEIQQANASLVLYEMKAARIEDQLRGCSDH 720
            RDDYNIKLVLEGVRARQ+QEIMLIEKQ LE E+QQ NASLVLYEMKAARIEDQLRGCSD 
Sbjct: 1166 RDDYNIKLVLEGVRARQLQEIMLIEKQALENEVQQGNASLVLYEMKAARIEDQLRGCSDQ 1225

Query: 721  IQKIEDNKLQDTDTLENSQKRLLDIRIASQQTRESLDQSQSKVEKCRTAQAELQIELEKE 780
            IQKIE++KL+DTDTLEN+QKRLL+IRIASQQTRESLDQS  KVEK RT QAELQIELEKE
Sbjct: 1226 IQKIEEDKLRDTDTLENTQKRLLEIRIASQQTRESLDQSHYKVEKSRTTQAELQIELEKE 1285

Query: 781  RFEKKRIEEELEVVGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQESLK 840
            RFEKKRIEEELEV+GRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQ  + 
Sbjct: 1286 RFEKKRIEEELEVIGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQVVIT 1345

Query: 841  KGPLYNERKSYKKTLQGSRKRKTPLPSEMEESPNKAKRSLISNASRSYQIGSDEVKGNGR 900
            K   +       + +  S+ RK P             RS                     
Sbjct: 1346 K-CFHLFCNPCVQDILKSQHRKCP-------------RSAF------------------- 1405

Query: 901  GAHGSRKYGCHRMKFQGCHYKVLPLVLLIRIASLLNTHGCGWTQTNKLYFAQPKNSTPTP 960
            GAH    +  H + +   + +++P    + I          W   +K YFAQP +  PTP
Sbjct: 1406 GAHFMAIWLRHELTWG--YKQIIPNCFTLLIQP--------WMDIDKAYFAQPNSCAPTP 1432

Query: 961  TVEARVDVWC 970
            TVEA VDVWC
Sbjct: 1466 TVEATVDVWC 1432

BLAST of Lag0020596 vs. NCBI nr
Match: XP_038902541.1 (E3 ubiquitin-protein ligase BRE1-like 1 [Benincasa hispida] >XP_038902548.1 E3 ubiquitin-protein ligase BRE1-like 1 [Benincasa hispida])

HSP 1 Score: 1404.0 bits (3633), Expect = 0.0e+00
Identity = 774/840 (92.14%), Postives = 804/840 (95.71%), Query Frame = 0

Query: 1   MGSTVDSDRKRRHFSTISPTAATAKKTPFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE 60
           MGSTV+SDRKRRHFSTISPTAATAKK PFLPISEDKKLDVAVLQYQNQKLIQKLE+QKVE
Sbjct: 1   MGSTVESDRKRRHFSTISPTAATAKKAPFLPISEDKKLDVAVLQYQNQKLIQKLEIQKVE 60

Query: 61  FKSLQNKYAQLKEKQEPYDMTVAVVKNCWEELVNGLETSSVRMRRWRSKQDGEHTMAAAD 120
           +KSLQNKYAQLKEKQEPYDMTVAVVKNCWEELVNGLETSSVRMRRWRSKQDGE T+AAAD
Sbjct: 61  YKSLQNKYAQLKEKQEPYDMTVAVVKNCWEELVNGLETSSVRMRRWRSKQDGEPTIAAAD 120

Query: 121 ESSSSFEDAVLSRLAETGATQSSSAYSSNEHMEEETESPSEKTKTIERNIEASIENLWYL 180
            S +SFEDAVLSRLAETGATQSSS YSS++HMEEETESP EKTKTIERNIEASIENLWYL
Sbjct: 121 GSLASFEDAVLSRLAETGATQSSSTYSSSKHMEEETESPCEKTKTIERNIEASIENLWYL 180

Query: 181 KDGLHAALLNELPKDDSFRNRAFGDLVKEVRNMRLRVKDFLFKQKGLAIELQNHRDLDAK 240
           KDGLHA LLNELPKDDSFR+R  GDLVKE RNMRLRVKDFLFKQK LA EL+NHRDL+AK
Sbjct: 181 KDGLHATLLNELPKDDSFRDRTSGDLVKEARNMRLRVKDFLFKQKALAKELENHRDLNAK 240

Query: 241 TKAELKVLKAELESAVAELEESNSKLTKLRAEHDAAKRDGFPVLNLSGKHSGGGKVRDKQ 300
           TKAELKVLK ELESAVAELEESNSKLTKLRAEHDAAK+ GFPVLNL+GKHSG GKVRDKQ
Sbjct: 241 TKAELKVLKVELESAVAELEESNSKLTKLRAEHDAAKKAGFPVLNLAGKHSGSGKVRDKQ 300

Query: 301 KDLRDMESSLKELKDQAVGRLAELNSLHEGRLKMLQQLSDIQNTMKSVKSISSSQSYLLL 360
           KDLRDMESSLKELKDQAV RLAELNSLHEGRLKML+QLSDI+NTMKSVKSISSS+SYLLL
Sbjct: 301 KDLRDMESSLKELKDQAVDRLAELNSLHEGRLKMLRQLSDIRNTMKSVKSISSSKSYLLL 360

Query: 361 RDRIEKLKLEVYEQQALFEKLQVEKDNIIWREKELNIKNNILDMLRRSSTVSDTRIDDLE 420
           RDRIE LK +V EQQALFEKLQ EKD+IIW+EKELNIKNNILD+LRRSSTVSDTRI+DLE
Sbjct: 361 RDRIENLKSQVNEQQALFEKLQDEKDSIIWKEKELNIKNNILDVLRRSSTVSDTRINDLE 420

Query: 421 MLIQKQKDEKKSMENKLAEVLKEPGRKKIISEFRALVSSFPEAMGSMQSQLHKYKEAASD 480
           MLIQKQKDEK+S+ENK+ EVLKEPGRKKIISEFRALVSSFPEAMGSMQSQLHKYKEAASD
Sbjct: 421 MLIQKQKDEKRSIENKMVEVLKEPGRKKIISEFRALVSSFPEAMGSMQSQLHKYKEAASD 480

Query: 481 VHSVRADMQSLSSIIDRMEKECESLSSRSKDQIAEIQKLQATVQDLTEVNRELKLIIDMY 540
           VHSVRADMQSLSSIIDRME ECESLSSRSKDQIAEIQKL+ATVQDLTEVNRELKLIIDMY
Sbjct: 481 VHSVRADMQSLSSIIDRMEMECESLSSRSKDQIAEIQKLEATVQDLTEVNRELKLIIDMY 540

Query: 541 RRESTESREVLEARDLEYKAWAHVQSLKSSLDERNLESRVKIANEAEAISQQRLAAAEAE 600
            RESTESREVLEARDLEYKAWA VQSLKSSLDERNLESRVK ANEAEAISQQRLAAAEAE
Sbjct: 541 SRESTESREVLEARDLEYKAWARVQSLKSSLDERNLESRVKTANEAEAISQQRLAAAEAE 600

Query: 601 IARLRQKLEASKRDLTRLTDALKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITER 660
           IARLRQKLEASKRDLTRL+D LKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITER
Sbjct: 601 IARLRQKLEASKRDLTRLSDVLKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITER 660

Query: 661 DDYNIKLVLEGVRARQMQEIMLIEKQTLEKEIQQANASLVLYEMKAARIEDQLRGCSDHI 720
           DDYNIKLVLEGVRARQ QE MLIEKQTLEKE+QQANASLVLYEMKAARIEDQLRGCSDH+
Sbjct: 661 DDYNIKLVLEGVRARQSQESMLIEKQTLEKEVQQANASLVLYEMKAARIEDQLRGCSDHV 720

Query: 721 QKIEDNKLQDTDTLENSQKRLLDIRIASQQTRESLDQSQSKVEKCRTAQAELQIELEKER 780
           QKIED KL DTDTLEN+QKRLLDIRIASQQTRESLDQSQSKVEK R  QAELQIELEKER
Sbjct: 721 QKIEDGKLHDTDTLENTQKRLLDIRIASQQTRESLDQSQSKVEKSRITQAELQIELEKER 780

Query: 781 FEKKRIEEELEVVGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQESLKK 840
           FEKKRIEEELEV+GRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQ  + K
Sbjct: 781 FEKKRIEEELEVIGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQVVITK 840

BLAST of Lag0020596 vs. NCBI nr
Match: XP_008437807.1 (PREDICTED: E3 ubiquitin-protein ligase BRE1-like 1 isoform X1 [Cucumis melo] >XP_008437808.1 PREDICTED: E3 ubiquitin-protein ligase BRE1-like 1 isoform X1 [Cucumis melo] >XP_008437809.1 PREDICTED: E3 ubiquitin-protein ligase BRE1-like 1 isoform X1 [Cucumis melo])

HSP 1 Score: 1395.6 bits (3611), Expect = 0.0e+00
Identity = 771/840 (91.79%), Postives = 801/840 (95.36%), Query Frame = 0

Query: 1   MGSTVDSDRKRRHFSTISPTAATAKKTPFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE 60
           MGSTV+SDRKRRHFSTISPTAATAKK PFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE
Sbjct: 1   MGSTVESDRKRRHFSTISPTAATAKKAPFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE 60

Query: 61  FKSLQNKYAQLKEKQEPYDMTVAVVKNCWEELVNGLETSSVRMRRWRSKQDGEHTMAAAD 120
           +KSLQNKYAQLKEKQEPYD TVAVVKNCWEELVNGLETSSVRMRRWRSK+DGEHT+AA D
Sbjct: 61  YKSLQNKYAQLKEKQEPYDTTVAVVKNCWEELVNGLETSSVRMRRWRSKRDGEHTIAAVD 120

Query: 121 ESSSSFEDAVLSRLAETGATQSSSAYSSNEHMEEETESPSEKTKTIERNIEASIENLWYL 180
            SSSSFEDAVLSRLAETGATQSSSAYSS++HMEEETESP EKTKTIERNIE SIENLWYL
Sbjct: 121 GSSSSFEDAVLSRLAETGATQSSSAYSSSKHMEEETESPCEKTKTIERNIETSIENLWYL 180

Query: 181 KDGLHAALLNELPKDDSFRNRAFGDLVKEVRNMRLRVKDFLFKQKGLAIELQNHRDLDAK 240
           KDGLHA LL+ELPKDDSFRNR  GDLVKEVRNMRLRVKDFLFKQK LA EL+ HRDLDAK
Sbjct: 181 KDGLHATLLSELPKDDSFRNRTSGDLVKEVRNMRLRVKDFLFKQKVLAKELEKHRDLDAK 240

Query: 241 TKAELKVLKAELESAVAELEESNSKLTKLRAEHDAAKRDGFPVLNLSGKHSGGGKVRDKQ 300
           TKAELKVLK ELESAVAELEESNSKLTKLRAEHDAAK+ GFPVLNL+GKHS  GKVRDKQ
Sbjct: 241 TKAELKVLKVELESAVAELEESNSKLTKLRAEHDAAKKAGFPVLNLAGKHSASGKVRDKQ 300

Query: 301 KDLRDMESSLKELKDQAVGRLAELNSLHEGRLKMLQQLSDIQNTMKSVKSISSSQSYLLL 360
           KDLRDME SLKELKDQAV RLAELNSLHEGRLKML++LSDIQNTMKSVKSISSS+ YLLL
Sbjct: 301 KDLRDMEFSLKELKDQAVDRLAELNSLHEGRLKMLRRLSDIQNTMKSVKSISSSKPYLLL 360

Query: 361 RDRIEKLKLEVYEQQALFEKLQVEKDNIIWREKELNIKNNILDMLRRSSTVSDTRIDDLE 420
           RDRIEKLK EV EQQALFEKLQVEKDNIIW+EKELNIKNNILD+LRRSSTVSDTRI+DLE
Sbjct: 361 RDRIEKLKSEVNEQQALFEKLQVEKDNIIWKEKELNIKNNILDVLRRSSTVSDTRINDLE 420

Query: 421 MLIQKQKDEKKSMENKLAEVLKEPGRKKIISEFRALVSSFPEAMGSMQSQLHKYKEAASD 480
           +LIQKQKD KKS+ENKL EVLKEPGRKKI+SEFRALVSSFPEAMGSMQSQLHKYKEAASD
Sbjct: 421 ILIQKQKDGKKSIENKLVEVLKEPGRKKIVSEFRALVSSFPEAMGSMQSQLHKYKEAASD 480

Query: 481 VHSVRADMQSLSSIIDRMEKECESLSSRSKDQIAEIQKLQATVQDLTEVNRELKLIIDMY 540
           VHSVRADMQSLSSIIDRMEKECESLSSRSKDQ AEIQKLQATVQDLTEVNRELKLIIDMY
Sbjct: 481 VHSVRADMQSLSSIIDRMEKECESLSSRSKDQHAEIQKLQATVQDLTEVNRELKLIIDMY 540

Query: 541 RRESTESREVLEARDLEYKAWAHVQSLKSSLDERNLESRVKIANEAEAISQQRLAAAEAE 600
            RESTESREVLEARDLEYKAWA VQSLKSSLDERNLESRVK ANE+EAISQQRLAAAEAE
Sbjct: 541 SRESTESREVLEARDLEYKAWARVQSLKSSLDERNLESRVKTANESEAISQQRLAAAEAE 600

Query: 601 IARLRQKLEASKRDLTRLTDALKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITER 660
           IARLRQKLEASKRDLTRL+D LKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITER
Sbjct: 601 IARLRQKLEASKRDLTRLSDVLKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITER 660

Query: 661 DDYNIKLVLEGVRARQMQEIMLIEKQTLEKEIQQANASLVLYEMKAARIEDQLRGCSDHI 720
           DDYNIKLVLEGVRARQ+QEIMLIEKQ LE E+QQ NASLVLYEMKAARIEDQLRGCSD I
Sbjct: 661 DDYNIKLVLEGVRARQLQEIMLIEKQALENEVQQGNASLVLYEMKAARIEDQLRGCSDQI 720

Query: 721 QKIEDNKLQDTDTLENSQKRLLDIRIASQQTRESLDQSQSKVEKCRTAQAELQIELEKER 780
           QKIE++KL+DTDTLEN+QKRLL+IRIASQQTRESLDQS  KVEK RT QAELQIELEKER
Sbjct: 721 QKIEEDKLRDTDTLENTQKRLLEIRIASQQTRESLDQSHYKVEKSRTTQAELQIELEKER 780

Query: 781 FEKKRIEEELEVVGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQESLKK 840
           FEKKRIEEELEV+GRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQ  + K
Sbjct: 781 FEKKRIEEELEVIGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQVVITK 840

BLAST of Lag0020596 vs. NCBI nr
Match: XP_011650646.1 (E3 ubiquitin-protein ligase BRE1-like 1 isoform X1 [Cucumis sativus] >KGN56365.1 hypothetical protein Csa_010435 [Cucumis sativus])

HSP 1 Score: 1389.4 bits (3595), Expect = 0.0e+00
Identity = 763/840 (90.83%), Postives = 802/840 (95.48%), Query Frame = 0

Query: 1   MGSTVDSDRKRRHFSTISPTAATAKKTPFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE 60
           MGSTV+SDRKRRHFSTISPTAATAKK PFLP+SEDKKLDVAVLQYQNQKLIQKLEVQKVE
Sbjct: 1   MGSTVESDRKRRHFSTISPTAATAKKAPFLPVSEDKKLDVAVLQYQNQKLIQKLEVQKVE 60

Query: 61  FKSLQNKYAQLKEKQEPYDMTVAVVKNCWEELVNGLETSSVRMRRWRSKQDGEHTMAAAD 120
           +KSLQNKYAQLKEKQEPYD TVAVVKNCWEELVNGLETSSVRMRRWRSK+DGEHT+A  D
Sbjct: 61  YKSLQNKYAQLKEKQEPYDTTVAVVKNCWEELVNGLETSSVRMRRWRSKRDGEHTIAGVD 120

Query: 121 ESSSSFEDAVLSRLAETGATQSSSAYSSNEHMEEETESPSEKTKTIERNIEASIENLWYL 180
            SSSSFEDAVLSRLAETGATQSSS YSS++HMEEETESP EKTKTIER+IE SIENLWYL
Sbjct: 121 GSSSSFEDAVLSRLAETGATQSSSTYSSSKHMEEETESPCEKTKTIERSIETSIENLWYL 180

Query: 181 KDGLHAALLNELPKDDSFRNRAFGDLVKEVRNMRLRVKDFLFKQKGLAIELQNHRDLDAK 240
           KDGLHA LLNELPKDDSFR R  GDLVKEVRNMRLRVKDFLFKQK LA EL+ HRDLDAK
Sbjct: 181 KDGLHATLLNELPKDDSFRKRTSGDLVKEVRNMRLRVKDFLFKQKVLAQELEKHRDLDAK 240

Query: 241 TKAELKVLKAELESAVAELEESNSKLTKLRAEHDAAKRDGFPVLNLSGKHSGGGKVRDKQ 300
           TKAELKVLK EL SAVAELEESNSKLTKLRAEHDAAK+ GFPVLNL+GKHS  GKVRDKQ
Sbjct: 241 TKAELKVLKVELGSAVAELEESNSKLTKLRAEHDAAKKAGFPVLNLTGKHSASGKVRDKQ 300

Query: 301 KDLRDMESSLKELKDQAVGRLAELNSLHEGRLKMLQQLSDIQNTMKSVKSISSSQSYLLL 360
           KDLRDMESSLKELKDQAV RLAELNSLHEGRLKML++LSDIQNTMKSVK+ISSS+ YLLL
Sbjct: 301 KDLRDMESSLKELKDQAVDRLAELNSLHEGRLKMLRRLSDIQNTMKSVKTISSSKPYLLL 360

Query: 361 RDRIEKLKLEVYEQQALFEKLQVEKDNIIWREKELNIKNNILDMLRRSSTVSDTRIDDLE 420
           RDRIEKLKLEV EQQALFEKLQVEKDNI+W+EKELNIKNNILD+LRRSSTVSDTRI+DLE
Sbjct: 361 RDRIEKLKLEVNEQQALFEKLQVEKDNIMWKEKELNIKNNILDVLRRSSTVSDTRINDLE 420

Query: 421 MLIQKQKDEKKSMENKLAEVLKEPGRKKIISEFRALVSSFPEAMGSMQSQLHKYKEAASD 480
           +LIQKQKD K+S+ENKL EVLKEPGRKKI+SEFRALVSSFPEAMGSMQSQLHKYKEAASD
Sbjct: 421 ILIQKQKDGKQSIENKLVEVLKEPGRKKIVSEFRALVSSFPEAMGSMQSQLHKYKEAASD 480

Query: 481 VHSVRADMQSLSSIIDRMEKECESLSSRSKDQIAEIQKLQATVQDLTEVNRELKLIIDMY 540
           VHSVRAD+QSLSSIIDRMEKECE+LSSRSKDQ AEIQKLQATVQDLTEVNRELKLIIDMY
Sbjct: 481 VHSVRADLQSLSSIIDRMEKECENLSSRSKDQQAEIQKLQATVQDLTEVNRELKLIIDMY 540

Query: 541 RRESTESREVLEARDLEYKAWAHVQSLKSSLDERNLESRVKIANEAEAISQQRLAAAEAE 600
            RESTESREVLEARDLEYKAWA VQSLKSSLDERNLESRVK ANEAEAISQQRLAAAEAE
Sbjct: 541 SRESTESREVLEARDLEYKAWARVQSLKSSLDERNLESRVKTANEAEAISQQRLAAAEAE 600

Query: 601 IARLRQKLEASKRDLTRLTDALKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITER 660
           IARLRQKLEASKRDLTRL+D LKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITER
Sbjct: 601 IARLRQKLEASKRDLTRLSDVLKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITER 660

Query: 661 DDYNIKLVLEGVRARQMQEIMLIEKQTLEKEIQQANASLVLYEMKAARIEDQLRGCSDHI 720
           DDYNIKLVLEGVRARQ+QEIMLIEKQ LE E+QQANASLVLYEMKAARIEDQLRGCSDHI
Sbjct: 661 DDYNIKLVLEGVRARQLQEIMLIEKQALENEVQQANASLVLYEMKAARIEDQLRGCSDHI 720

Query: 721 QKIEDNKLQDTDTLENSQKRLLDIRIASQQTRESLDQSQSKVEKCRTAQAELQIELEKER 780
           QKIE++KL+DTDTLEN++KRLL+IRIASQQTRESLD+ QSKVE+ RT QAELQIELEKER
Sbjct: 721 QKIEEDKLRDTDTLENTRKRLLEIRIASQQTRESLDECQSKVERSRTTQAELQIELEKER 780

Query: 781 FEKKRIEEELEVVGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQESLKK 840
           FEKKRIEEELEV+GRKASRLEAQMESSSV+EKLHEELGEYEKIVNCKICVNSRKQ  + K
Sbjct: 781 FEKKRIEEELEVIGRKASRLEAQMESSSVIEKLHEELGEYEKIVNCKICVNSRKQVVITK 840

BLAST of Lag0020596 vs. NCBI nr
Match: XP_023526841.1 (E3 ubiquitin-protein ligase BRE1-like 1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1384.8 bits (3583), Expect = 0.0e+00
Identity = 764/840 (90.95%), Postives = 797/840 (94.88%), Query Frame = 0

Query: 1   MGSTVDSDRKRRHFSTISPTAATAKKTPFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE 60
           MGSTV+SDRKRRHFSTISPTAATAKK PFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE
Sbjct: 1   MGSTVESDRKRRHFSTISPTAATAKKAPFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE 60

Query: 61  FKSLQNKYAQLKEKQEPYDMTVAVVKNCWEELVNGLETSSVRMRRWRSKQDGEHTMAAAD 120
           +KSLQNKYA LKEKQEPYD+TVAVVKNCWEELVNGLE SSV MRRWRSKQDGE ++AA D
Sbjct: 61  YKSLQNKYAHLKEKQEPYDITVAVVKNCWEELVNGLEKSSVCMRRWRSKQDGEQSIAAVD 120

Query: 121 ESSSSFEDAVLSRLAETGATQSSSAYSSNEHMEEETESPSEKTKTIERNIEASIENLWYL 180
            SSSSFEDAVLSRLAETGATQSSS+YSS +HMEEETESP EKTKTIERNIEASIENLWYL
Sbjct: 121 GSSSSFEDAVLSRLAETGATQSSSSYSSTKHMEEETESPCEKTKTIERNIEASIENLWYL 180

Query: 181 KDGLHAALLNELPKDDSFRNRAFGDLVKEVRNMRLRVKDFLFKQKGLAIELQNHRDLDAK 240
           KDGLHA LL+ELP DDSFRNR  GDLVKEVRNMRLRVKDFLFKQK LA ELQNHRDLDAK
Sbjct: 181 KDGLHATLLSELPIDDSFRNRTSGDLVKEVRNMRLRVKDFLFKQKVLAKELQNHRDLDAK 240

Query: 241 TKAELKVLKAELESAVAELEESNSKLTKLRAEHDAAKRDGFPVLNLSGKHSGGGKVRDKQ 300
           TKAELK LK ELESAVAELE+SNSKLTKLRAEHDAAK+ GFPVLNL+GKHS   KVRDKQ
Sbjct: 241 TKAELKALKVELESAVAELEDSNSKLTKLRAEHDAAKKAGFPVLNLAGKHSSSSKVRDKQ 300

Query: 301 KDLRDMESSLKELKDQAVGRLAELNSLHEGRLKMLQQLSDIQNTMKSVKSISSSQSYLLL 360
           KDLRDMES+LKELKDQAV RLAELNSLHEGRLKMLQQLSD +NTMKSVK +S+SQSYLLL
Sbjct: 301 KDLRDMESALKELKDQAVDRLAELNSLHEGRLKMLQQLSDFRNTMKSVKCLSASQSYLLL 360

Query: 361 RDRIEKLKLEVYEQQALFEKLQVEKDNIIWREKELNIKNNILDMLRRSSTVSDTRIDDLE 420
           RDRIEKLK EVYEQQALFEKLQVEKDNIIWREKELN+KNNILD+LRRSSTVSDT+I+DLE
Sbjct: 361 RDRIEKLKSEVYEQQALFEKLQVEKDNIIWREKELNVKNNILDVLRRSSTVSDTKINDLE 420

Query: 421 MLIQKQKDEKKSMENKLAEVLKEPGRKKIISEFRALVSSFPEAMGSMQSQLHKYKEAASD 480
           MLIQK KDEK S ENKL EVLKEPGRKKI+SEFRALVSSFPEAMGSMQSQLHKYKEAASD
Sbjct: 421 MLIQKHKDEKMSTENKLVEVLKEPGRKKIVSEFRALVSSFPEAMGSMQSQLHKYKEAASD 480

Query: 481 VHSVRADMQSLSSIIDRMEKECESLSSRSKDQIAEIQKLQATVQDLTEVNRELKLIIDMY 540
           VHSVRA MQSLSSIIDRMEKECE LSS+SKDQIAEIQKLQATVQDLTE NR+LKLIIDMY
Sbjct: 481 VHSVRAFMQSLSSIIDRMEKECEGLSSQSKDQIAEIQKLQATVQDLTEFNRDLKLIIDMY 540

Query: 541 RRESTESREVLEARDLEYKAWAHVQSLKSSLDERNLESRVKIANEAEAISQQRLAAAEAE 600
           RRESTESREVLEARDLEYKAWA VQSLKSSLDERNLESRVK ANEAEAISQQRLAAAEAE
Sbjct: 541 RRESTESREVLEARDLEYKAWARVQSLKSSLDERNLESRVKTANEAEAISQQRLAAAEAE 600

Query: 601 IARLRQKLEASKRDLTRLTDALKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITER 660
           IARLRQKLEASKRDLTRL+D LKSK DENVAYLSEIETIGQAYDDMQTQNQHLLQQITER
Sbjct: 601 IARLRQKLEASKRDLTRLSDVLKSKSDENVAYLSEIETIGQAYDDMQTQNQHLLQQITER 660

Query: 661 DDYNIKLVLEGVRARQMQEIMLIEKQTLEKEIQQANASLVLYEMKAARIEDQLRGCSDHI 720
           DDYNIKLVLEGVRARQ+QEIMLIEKQTLEKEIQQANASLVL+EMKAAR+EDQLRGCSDHI
Sbjct: 661 DDYNIKLVLEGVRARQLQEIMLIEKQTLEKEIQQANASLVLFEMKAARVEDQLRGCSDHI 720

Query: 721 QKIEDNKLQDTDTLENSQKRLLDIRIASQQTRESLDQSQSKVEKCRTAQAELQIELEKER 780
           +KI+D+KL DTDTLEN QKRLLDI++ASQQTRESLDQSQSKVEK RT QAELQIELEKER
Sbjct: 721 KKIKDDKLHDTDTLENIQKRLLDIKLASQQTRESLDQSQSKVEKSRTTQAELQIELEKER 780

Query: 781 FEKKRIEEELEVVGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQESLKK 840
           FEKKRIEEELEVVGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICV+SRKQ  + K
Sbjct: 781 FEKKRIEEELEVVGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVDSRKQVVITK 840

BLAST of Lag0020596 vs. ExPASy Swiss-Prot
Match: Q8RXD6 (E3 ubiquitin-protein ligase BRE1-like 1 OS=Arabidopsis thaliana OX=3702 GN=HUB1 PE=1 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 3.0e-198
Identity = 422/866 (48.73%), Postives = 589/866 (68.01%), Query Frame = 0

Query: 1   MGSTVDSDRKRRHFSTISPT--AATAKKTPFLPISEDKKLDVAVLQYQNQKLIQKLEVQK 60
           M ST + DRKRRHFS+ISP+  AA  KK PF   S + KLD AVLQ+QN KL QKLE Q+
Sbjct: 1   MASTGEPDRKRRHFSSISPSEAAAAVKKQPFFWPSSEDKLDTAVLQFQNLKLSQKLEAQQ 60

Query: 61  VEFKSLQNKYAQLKEKQEPYDMTVAVVKNCWEELVNGLETSSVRMRRWRSKQDGEHTMA- 120
           VE   L++K +Q+KEKQ PY+ ++  V   WE+L   +E+ SVR+        G H    
Sbjct: 61  VECSILEDKLSQIKEKQLPYNSSLKTVHKSWEKLTASVESCSVRV---SDSSSGAHRFVN 120

Query: 121 AADESSSSFEDAVLSRLAETGATQSSSAYSSNEHMEEETESPSEKTKTIERNIEASIENL 180
             D SS + ++  ++RL ETGAT+SSS+   +  MEE   + S +      N+ A+ E+L
Sbjct: 121 KEDGSSPAVKNDFINRLLETGATESSSSNICSNQMEENGVNTSSQMTQTLYNLVAATEDL 180

Query: 181 WYLKDGLHAALLNELPKDDSFRNRAFGDLVKEVRNMRLRVKDFLFKQKGLAIELQNHRDL 240
             LKD L+  +L      D     A  +L  E+++ R  + D L K K L+ ELQ+HRD 
Sbjct: 181 RCLKDELYPTVLRTNLGKDLCGQLALSELESEIKSFRGDLDDVLVKFKSLSRELQSHRDA 240

Query: 241 DAKTKAELKVLKAELESAVAELEESNSKLTKLRAEHDAAKRDGFPVLNLSGKHSGGGKVR 300
           DAK + +LK ++ ELE  V EL++ N  L+ LRAE DA     FPVL+L  K +   + R
Sbjct: 241 DAKVRVDLKRIRGELEDEVVELQQCNGDLSALRAERDATAGAFFPVLSLGNKLATSDRER 300

Query: 301 DKQKDLRDMESSLKELKDQAVGRLAELNSLHEGRLKMLQQLSDIQNTMKSVKSISSSQSY 360
           DKQ+DL+DME+ LKEL   A GRL +L +LHE R KML ++S++QN  KSV+ ISSSQ+ 
Sbjct: 301 DKQRDLQDMETVLKELTVLASGRLQQLKNLHEERTKMLGKMSNLQNKSKSVRCISSSQAC 360

Query: 361 LLLRDRIEKLKLEVYEQQALFEKLQVEKDNIIWREKELNIKNNILDMLRRSSTVSDTRID 420
           L L+D++EK K  V++  AL EKLQVEKD+I+W+E+E+NIKN + D+ R++S V+D+R+ 
Sbjct: 361 LSLKDQLEKSKEAVFQYMALLEKLQVEKDSIVWKEREINIKNELGDVSRKTSAVTDSRMA 420

Query: 421 DLEMLIQKQKDEKKSMENKLAEVLKEPGRKKIISEFRALVSSFPEAMGSMQSQLHKYKEA 480
            L+  IQKQ DEK  ++ +L  + +E GRK+I ++ +AL+SSFPE M SM+SQL+ YKE 
Sbjct: 421 SLDSEIQKQLDEKMRIKTRLGNISRERGRKEIFADMKALISSFPEEMSSMRSQLNNYKET 480

Query: 481 ASDVHSVRADMQSLSSIIDRMEKECESLSSRSKDQIAEIQKLQATVQDLTEVNRELKLII 540
           A  +HS+RAD+QSLS ++ R  KE E+L  RS D  +++  L ATV DL   + ELKL +
Sbjct: 481 AGGIHSLRADVQSLSGVLCRKTKEYEALQLRSADYASQLGDLNATVCDLKNSHEELKLFL 540

Query: 541 DMYRRESTESREVLEARDLEYKAWAHVQSLKSSLDERNLESRVKIANEAEAISQQRLAAA 600
           DMY+REST++R++ EA++ EY+AWAHVQSLKSSLDE+NLE RVK ANEAEA+SQQ LAAA
Sbjct: 541 DMYKRESTDARDIAEAKEQEYRAWAHVQSLKSSLDEQNLELRVKAANEAEAVSQQMLAAA 600

Query: 601 EAEIARLRQKLEASKRDLTRLTDALKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQI 660
           EAEIA LRQK++  KRD+ + +D LKSK +E+  YLSEI+TIG AY+D+  QNQ LL Q+
Sbjct: 601 EAEIADLRQKMDDCKRDVAKHSDILKSKHEEHGTYLSEIQTIGSAYEDIVPQNQQLLLQV 660

Query: 661 TERDDYNIKLVLEGVRARQMQEIMLIEKQTLEKEIQQANASLVLYEMKAARIEDQLRGCS 720
           TERDDYNIKL LEG+ +RQMQ+ +LI+K  ++K+IQQ +A       K++RIEDQLR C+
Sbjct: 661 TERDDYNIKLFLEGITSRQMQDTLLIDKYIMDKDIQQGSAYASFLSKKSSRIEDQLRFCT 720

Query: 721 DHIQKIEDNKLQDTDTLENSQKRLLDIRIASQQTRESLDQSQSKVEKCRTAQAELQIELE 780
           D  QK+ ++K Q + +LEN QK+  DI    +Q R  L++S SKVE+ R     L++ELE
Sbjct: 721 DQFQKLAEDKYQKSVSLENLQKKRADIGNGLEQARSRLEESHSKVEQSRLDYGALELELE 780

Query: 781 KERFEKKRIEEELEVVGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQES 840
            ERF ++RIEEE+E+  +K SRL + +E SS ++KL +EL E+++I+ CK C N R +E 
Sbjct: 781 IERFNRRRIEEEMEIAKKKVSRLRSLIEGSSAIQKLRQELSEFKEILKCKAC-NDRPKEV 840

Query: 841 LKKGPLYNERKSYKKTLQGSRKRKTP 864
           +     +       + L G+R++K P
Sbjct: 841 VITKCYHLFCNPCVQKLTGTRQKKCP 862

BLAST of Lag0020596 vs. ExPASy Swiss-Prot
Match: A2XW69 (E3 ubiquitin-protein ligase BRE1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=BRE1A PE=3 SV=2)

HSP 1 Score: 573.9 bits (1478), Expect = 3.5e-162
Identity = 367/870 (42.18%), Postives = 550/870 (63.22%), Query Frame = 0

Query: 1   MGSTVDSDRKRRHFSTISP----TAATAKKTPFLPISEDKKLDVAVLQYQNQKLIQKLEV 60
           MGST + DRKRR  S+++P      + AK+    P SEDKKLD  VL+Y+NQKL ++LE 
Sbjct: 1   MGSTGEPDRKRRLSSSVAPGGGAPVSPAKRLAVAPTSEDKKLDFTVLKYKNQKLSEQLEA 60

Query: 61  QKVEFKSLQNKYAQLKEKQEPYDMTVAVVKNCWEELVNGLETSS-VRMRRWRSKQDGEHT 120
            K E+++L+NK+A LKEKQ  ++ T+++V + WE+LV  L++ S  +     S     H 
Sbjct: 61  HKFEYRALENKFAGLKEKQRTHNETLSLVNSSWEQLVADLKSRSFCKSGSPNSSPGSGHN 120

Query: 121 MAAADESSSSFEDAVLSRLAETGATQSSSAYSSNEHMEEETESPSEKTKTIERNIEASIE 180
               D + +  E   L  L E+GAT+SS       H+  +       T     +I     
Sbjct: 121 NVQKDGTCAPIERDTLRSLVESGATESSGCLPGC-HLGSDAPPLHLSTANALGDIFFPSS 180

Query: 181 NLWYLKDGLHAALLNELPKDDSFR--NRAFGDLVKEVRNMRLRVKDFLFKQKGLAIELQN 240
           +L    +    A L +LP++D  +       +L+  + N+   + +   K K LA + QN
Sbjct: 181 DLLQANEECALAALTKLPENDRSKQLQSTSSNLLSSLNNVVQALSNLQLKHKQLAEDYQN 240

Query: 241 HRDLDAKTKAELKVLKAELESAVAELEESNSKLTKLRAEHDAAKRDGFPVLNLSGKHSGG 300
            RD  A+ +AE + LK EL SA +ELEE+N KL  L+A+ D  +    P   L  K    
Sbjct: 241 QRDSSARKRAEHRRLKEELASAASELEETNYKLAALKAQRDNTQGARIPYPTLGNKSMPE 300

Query: 301 GKVRDKQKDLRDMESSLKELKDQAVGRLAELNSLHEGRLKMLQQLSDIQNTMKSVKSISS 360
            KVRDKQ++++D+E++ KEL +    RL E+  LHE R+++L +++  QN +   KSI S
Sbjct: 301 DKVRDKQREMQDLEATHKELSELISKRLVEIKRLHEERIEILNKIATFQNILMDFKSIRS 360

Query: 361 SQSYLLLRDRIEKLKLEVYEQQALFEKLQVEKDNIIWREKELNIKNNILDMLRRSSTVSD 420
           S+++ L+ DR++K + E+   Q L EKLQV+KD  +W+E++ N+K ++ ++  R ST  +
Sbjct: 361 SKAFQLVNDRLQKSQAELDHYQTLLEKLQVDKDKFVWQERQFNLKVDLAEIPERVSTYCE 420

Query: 421 TRIDDLEMLIQKQKDEKKSMENKLAEVLKEPGRKKIISEFRALVSSFPEAMGSMQSQLHK 480
           + I DL+  IQK +DEK  +  KL E  +EPGR ++I++F+ALVSS P  MG+MQS++ K
Sbjct: 421 SSIADLKKDIQKLRDEKNMLILKLEEASREPGRNQVITKFKALVSSIPREMGAMQSEMTK 480

Query: 481 YKEAASDVHSVRADMQSLSSIIDRMEKECESLSSRSKDQIAEIQKLQATVQDLTEVNREL 540
           +KEA+ +++S+RA++ SLS I+ R E++ E  S RS    ++I +LQ+ + DL + N+EL
Sbjct: 481 HKEASLELNSLRAEVHSLSRILSRKERDNEEASCRSARAGSDITQLQSVISDLKQTNKEL 540

Query: 541 KLIIDMYRRESTESREVLEARDLEYKAWAHVQSLKSSLDERNLESRVKIANEAEAISQQR 600
           KL  DMY+REST+SRE++E+RD E+  WAHV +LKSSLDE  LE RVK ANEAEAI+QQR
Sbjct: 541 KLFADMYKRESTDSREIMESRDREFLEWAHVHALKSSLDESKLEQRVKAANEAEAITQQR 600

Query: 601 LAAAEAEIARLRQKLEASKRDLTRLTDALKSKGDENVAYLSEIETIGQAYDDMQTQNQHL 660
           LA AEAEIA   QKL  S++DL  L+  LKSK +E  AY  E+E IGQAY+D+Q QNQ L
Sbjct: 601 LATAEAEIAESGQKLGTSRKDLVSLSHMLKSKQEECEAYRVEVECIGQAYEDIQAQNQQL 660

Query: 661 LQQITERDDYNIKLVLEGVRARQMQEIMLIEKQTLEKEIQQANASLVLYEMKAARIEDQL 720
           LQQI ERDD N K+ +EGV+A+Q Q+ + +E  +L + +QQ ++ + LY  K   +EDQL
Sbjct: 661 LQQIIERDDDNTKIFMEGVKAKQTQDALHLETYSLRRNLQQESSLMDLYNQKIVSLEDQL 720

Query: 721 RGCSDHIQKIEDNKLQDTDTLENSQKRLLDIRIASQQTRESLDQSQSKVEKCRTAQAELQ 780
           +  SD + K++++  Q + +L N Q++L+D+   +Q+  +SLD  Q+ V   R   A+L 
Sbjct: 721 KMWSDRVGKLQEDGWQQSVSLSNYQRKLVDVHRDAQKLMQSLDGIQANVGSSRLEVADLL 780

Query: 781 IELEKERFEKKRIEEELEVVGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSR 840
           IELEKERF KKRIE++LEV+ RKAS L A+   S+V+EKL  E+ EY  I+ C IC + +
Sbjct: 781 IELEKERFSKKRIEDDLEVMSRKASSLRAKARESAVLEKLRHEVKEYRGILKCGICHDRQ 840

Query: 841 KQESLKKGPLYNERKSYKKTLQGSRKRKTP 864
           K+  + K       +  +K+L G+R+R+ P
Sbjct: 841 KEVVITKCYHLFCNQCIQKSL-GNRQRRCP 868

BLAST of Lag0020596 vs. ExPASy Swiss-Prot
Match: Q7XU27 (E3 ubiquitin-protein ligase BRE1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=HUB1 PE=1 SV=3)

HSP 1 Score: 572.8 bits (1475), Expect = 7.7e-162
Identity = 367/870 (42.18%), Postives = 550/870 (63.22%), Query Frame = 0

Query: 1   MGSTVDSDRKRRHFSTISP----TAATAKKTPFLPISEDKKLDVAVLQYQNQKLIQKLEV 60
           MGST + DRKRR  S+++P      + AK+    P SEDKKLD  VL+Y+NQKL ++LE 
Sbjct: 1   MGSTGEPDRKRRLSSSVAPGGGAPVSPAKRLAVAPTSEDKKLDFTVLKYKNQKLSEQLEA 60

Query: 61  QKVEFKSLQNKYAQLKEKQEPYDMTVAVVKNCWEELVNGLETSS-VRMRRWRSKQDGEHT 120
            K E+++L+NK+A LKEKQ  ++ T+++V + WE+LV  L++ S  +     S     H 
Sbjct: 61  HKFEYRALENKFAGLKEKQRTHNETLSLVNSSWEQLVADLKSRSFCKSGSPNSSPGSGHN 120

Query: 121 MAAADESSSSFEDAVLSRLAETGATQSSSAYSSNEHMEEETESPSEKTKTIERNIEASIE 180
               D + +  E   L  L E+GAT+SS       H+  +       T     +I     
Sbjct: 121 NVQKDGTCAPIERDTLRSLVESGATESSGCLPGC-HLGSDAPPLHLSTANALGDIFFPSS 180

Query: 181 NLWYLKDGLHAALLNELPKDDSFR--NRAFGDLVKEVRNMRLRVKDFLFKQKGLAIELQN 240
           +L    +    A L +LP++D  +       +L+  + N+   + +   K K LA + QN
Sbjct: 181 DLLQANEECALAALTKLPENDRSKQLQSTSSNLLSSLNNVVQALSNLQLKHKQLAEDYQN 240

Query: 241 HRDLDAKTKAELKVLKAELESAVAELEESNSKLTKLRAEHDAAKRDGFPVLNLSGKHSGG 300
            RD  A+ +AE + LK EL SA +ELEE+N KL  L+A+ D  +    P   L  K+   
Sbjct: 241 QRDSSARKRAEHRRLKEELASAASELEETNYKLAALKAQRDNTQGARIPYPTLGNKNMPE 300

Query: 301 GKVRDKQKDLRDMESSLKELKDQAVGRLAELNSLHEGRLKMLQQLSDIQNTMKSVKSISS 360
            KVRDKQ++++D+E++ KEL +    RL E+  LHE R+++L +++  QN +   KSI S
Sbjct: 301 DKVRDKQREMQDLEATHKELSELISKRLVEIKRLHEERIEILNKIATFQNILMDFKSIRS 360

Query: 361 SQSYLLLRDRIEKLKLEVYEQQALFEKLQVEKDNIIWREKELNIKNNILDMLRRSSTVSD 420
           S+++ L+ DR++K + E+   Q L EKLQV+KD  +W+E++ N+K ++ ++  R ST  +
Sbjct: 361 SKAFQLVNDRLQKSQAELDHYQTLLEKLQVDKDKFVWQERQFNLKVDLAEIPERVSTYCE 420

Query: 421 TRIDDLEMLIQKQKDEKKSMENKLAEVLKEPGRKKIISEFRALVSSFPEAMGSMQSQLHK 480
           + I DL+  IQK  DEK  +  KL E  +EPGR ++I++F+ALVSS P  MG+MQS++ K
Sbjct: 421 SSIADLKKDIQKLCDEKNMLILKLEEASREPGRNQVITKFKALVSSIPREMGAMQSEMTK 480

Query: 481 YKEAASDVHSVRADMQSLSSIIDRMEKECESLSSRSKDQIAEIQKLQATVQDLTEVNREL 540
           +KEA+ +++S+RA++ SLS I+ R E++ E  S RS    ++I +LQ+ + DL + N+EL
Sbjct: 481 HKEASLELNSLRAEVHSLSRILSRKERDNEEASCRSARAGSDITQLQSVISDLKQTNKEL 540

Query: 541 KLIIDMYRRESTESREVLEARDLEYKAWAHVQSLKSSLDERNLESRVKIANEAEAISQQR 600
           KL  DMY+REST+SRE++E+RD E+  WAHV +LKSSLDE  LE RVK ANEAEAI+QQR
Sbjct: 541 KLFADMYKRESTDSREIMESRDREFLEWAHVHALKSSLDESKLEQRVKAANEAEAITQQR 600

Query: 601 LAAAEAEIARLRQKLEASKRDLTRLTDALKSKGDENVAYLSEIETIGQAYDDMQTQNQHL 660
           LA AEAEIA   QKL  S++DL  L+  LKSK +E  AY  E+E IGQAY+D+Q QNQ L
Sbjct: 601 LATAEAEIAESGQKLGTSRKDLVSLSHMLKSKQEECEAYRVEVECIGQAYEDIQAQNQQL 660

Query: 661 LQQITERDDYNIKLVLEGVRARQMQEIMLIEKQTLEKEIQQANASLVLYEMKAARIEDQL 720
           LQQI ERDD N K+ +EGV+A+Q Q+ + +E  +L + +QQ ++ + LY  K   +EDQL
Sbjct: 661 LQQIIERDDDNTKIFMEGVKAKQTQDALHLETYSLRRNLQQESSLMDLYNQKIVSLEDQL 720

Query: 721 RGCSDHIQKIEDNKLQDTDTLENSQKRLLDIRIASQQTRESLDQSQSKVEKCRTAQAELQ 780
           +  SD + K++++  Q + +L N Q++L+D+   +Q+  +SLD  Q+ V   R   A+L 
Sbjct: 721 KMWSDRVGKLQEDGWQQSVSLSNYQRKLVDVHRDAQKLMQSLDGIQANVGSSRLEVADLL 780

Query: 781 IELEKERFEKKRIEEELEVVGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSR 840
           IELEKERF KKRIE++LEV+ RKAS L A+   S+V+EKL  E+ EY  I+ C IC + +
Sbjct: 781 IELEKERFSKKRIEDDLEVMSRKASSLRAKARESAVLEKLRHEVKEYRGILKCGICHDRQ 840

Query: 841 KQESLKKGPLYNERKSYKKTLQGSRKRKTP 864
           K+  + K       +  +K+L G+R+R+ P
Sbjct: 841 KEVVITKCYHLFCNQCIQKSL-GNRQRRCP 868

BLAST of Lag0020596 vs. ExPASy Swiss-Prot
Match: A2ZAC2 (E3 ubiquitin-protein ligase BRE1-like 2 OS=Oryza sativa subsp. indica OX=39946 GN=BRE1B PE=3 SV=2)

HSP 1 Score: 349.4 bits (895), Expect = 1.4e-94
Identity = 267/815 (32.76%), Postives = 449/815 (55.09%), Query Frame = 0

Query: 38  LDVAVLQYQNQKLIQKLEVQKVEFKSLQNKYAQLKEKQEPYDMTVAVVKNCWEELVNGLE 97
           +D A LQY+NQKL+Q+LE QK + ++L+ K+ +L+++Q  YD T+  +   W +L++ L 
Sbjct: 1   MDAAALQYENQKLVQQLEAQKSKMRALEGKFKELRDEQCSYDNTLICLNKMWNQLIDDLV 60

Query: 98  TSSVRM---RRWRSKQDGEHTMAAADESSSSFEDAVLSRLAETGATQSSSAYSSNEHMEE 157
              VR           D E     + ES  S E+  L RL  +   +++   S ++ +EE
Sbjct: 61  LLGVRAGGDLNGLQALDHEEMSEESLESCPS-EEIFLFRLLNSRNFRNNDDSSLSKLVEE 120

Query: 158 ETESPSEKTKTIERNIEASIENLWYLKDGLHAALLNELPKDD---SFRNRAFGDLVKE-V 217
                   T T+ ++++ +        + L  AL  +   +D   +  N    D +KE V
Sbjct: 121 ALALRYSTTVTLMKSLQEAFAVQQARSESLSLALNGQNSSEDVIVALENH--NDYLKEVV 180

Query: 218 RNMRLRVKDFLFKQKGLAIELQNHRDLDAKTKAELKVLKAELESAVAELEESNSKLTKLR 277
            N+R  V     K +    E++  ++  ++   E+K L  ELE ++AELEES  KL  L+
Sbjct: 181 DNLRQAVSIINRKHEKYLDEIEAFKNNQSRELHEVKCLSGELEESMAELEESRRKLAVLQ 240

Query: 278 AEHDAAKRDGFPVLNLSGKHSGGGKV-----RDKQKDLRDMESSLKELKDQAVGRLAELN 337
            +       G  ++N S  +   G V      DK    RD++ +++E K  A  RL EL+
Sbjct: 241 LQ-----TGGGSLMNTSAPNGVNGSVSTDKSSDKGMGWRDLKDAVEEAKTLAANRLFELH 300

Query: 338 SLHEGRLKMLQQLSDIQNTMKSVKSISSSQSYLLLRDRIEKLKLEVYEQQALFEKLQVEK 397
              E  L + +QL DIQ+ +K    I +S+ Y +L D++  L  E+   + L E LQ EK
Sbjct: 301 ETQEDNLILSKQLEDIQDQLKDENYIVTSKPYTILSDQLHHLNAEIERYRGLVEVLQNEK 360

Query: 398 DNIIWREKELNIKNNILDMLRRSSTVSDTRIDDLEMLIQKQKDEKKSMENKLAEVLKEPG 457
           D ++ +E+E+  K   +D +++S T    +I+DLE  IQK   EK  +E K  E L++ G
Sbjct: 361 DQLMQKEEEMLAKAESVDAVQQSITTYKAKIEDLEHEIQKLMAEKNDLEIKAEEALQDSG 420

Query: 458 RKKIISEFRALVSSFPEAMGSMQSQLHKYKEAASDVHSVRADMQSLSSIIDRMEKECESL 517
           +K    E   + +S  + M  + +Q+++ K+AAS+  ++R +   L +++ +   E + +
Sbjct: 421 KKDFKDEIHVMAASLSKEMELLDNQMNRSKDAASEALALREEADYLRTLLAKKIDEQKEI 480

Query: 518 SSRSKDQIAEIQKLQATVQDLTEVNRELKLIIDMYRRESTESREVLEARDLEYKAWAHVQ 577
           S R   Q+ EI+ L+A ++ L +  +EL+ I+DM  +E +ESR + E  + E +A    +
Sbjct: 481 SDRYNTQVTEIKSLKALIETLDQEKQELQFIVDMLGKECSESRAISEIEESENRARKQAE 540

Query: 578 SLKSSLDERNLESRVKIANEAEAISQQRLAAAEAEIARLRQKLEASKRDLTRLTDALKSK 637
            L+  L+E NLE RVK ANEAE   QQRL+ AEAE+  LR K++AS+RD+ +L ++++ K
Sbjct: 541 YLRKCLEEHNLELRVKAANEAETACQQRLSIAEAELEDLRAKVDASERDVMKLKESIRIK 600

Query: 638 GDENVAYLSEIETIGQAYDDMQTQNQHLLQQITERDDYNIKLVLEGVRARQMQEIMLIEK 697
             E   ++SEIETIGQAY+DMQTQNQHLLQQ+ +RDD+NIKLV + V+ +Q    +L EK
Sbjct: 601 EAEVDGHISEIETIGQAYEDMQTQNQHLLQQVADRDDFNIKLVSDSVKMKQAYGSLLAEK 660

Query: 698 QTLEKEIQQANASLVLYEMKAARIEDQLRGCSDHIQKIEDNKLQDTDTLENSQKRLLDIR 757
             L+K++Q  N+SL   ++K    E+Q++       K          +LE +   + D  
Sbjct: 661 NMLQKQLQHVNSSLESSKLKITSGEEQMKTYVAQAMKSSSENRHLAISLERTMLEVSDAE 720

Query: 758 IASQQTRESLDQSQSKVEKCRTAQAELQIELEKERFEKKRIEEELEVVGRKASRLEAQME 817
              +  R +   ++ + E  +   AEL++ELE+ER E+ ++EEE E V  + S L ++ E
Sbjct: 721 KELKWLRSATGSAEKEYEINQKKIAELKMELERERNERIKLEEEYEEVKNEVSELTSETE 780

Query: 818 SSSVVEKLHEELGEYEKIVNCKICVNSRKQESLKK 841
            ++ ++KL +E+ E + I+ C +C +  K+  + K
Sbjct: 781 ETT-IQKLQDEIKECKAILKCGVCFDRPKEVVITK 806

BLAST of Lag0020596 vs. ExPASy Swiss-Prot
Match: Q336R3 (E3 ubiquitin-protein ligase BRE1-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=HUB2 PE=1 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 1.4e-94
Identity = 267/815 (32.76%), Postives = 449/815 (55.09%), Query Frame = 0

Query: 38  LDVAVLQYQNQKLIQKLEVQKVEFKSLQNKYAQLKEKQEPYDMTVAVVKNCWEELVNGLE 97
           +D A LQY+NQKL+Q+LE QK + ++L+ K+ +L+++Q  YD T+  +   W +L++ L 
Sbjct: 1   MDAAALQYENQKLVQQLEAQKSKMRALEGKFKELRDEQCSYDNTLICLNKMWNQLIDDLV 60

Query: 98  TSSVRM---RRWRSKQDGEHTMAAADESSSSFEDAVLSRLAETGATQSSSAYSSNEHMEE 157
              VR           D E     + ES  S E+  L RL  +   +++   S ++ +EE
Sbjct: 61  LLGVRAGGDLNGLQALDHEEMSEESLESCPS-EEIFLFRLLNSRNFRNNDDSSLSKLVEE 120

Query: 158 ETESPSEKTKTIERNIEASIENLWYLKDGLHAALLNELPKDD---SFRNRAFGDLVKE-V 217
                   T T+ ++++ +        + L  AL  +   +D   +  N    D +KE V
Sbjct: 121 ALALRYSTTVTLMKSLQEAFAVQQARSESLSLALNGQNSSEDVIVALENH--NDYLKEVV 180

Query: 218 RNMRLRVKDFLFKQKGLAIELQNHRDLDAKTKAELKVLKAELESAVAELEESNSKLTKLR 277
            N+R  V     K +    E++  ++  ++   E+K L  ELE ++AELEES  KL  L+
Sbjct: 181 DNLRQAVSIINRKHEKYLDEIEAFKNNQSRELHEVKCLSGELEESMAELEESRRKLAVLQ 240

Query: 278 AEHDAAKRDGFPVLNLSGKHSGGGKV-----RDKQKDLRDMESSLKELKDQAVGRLAELN 337
            +       G  ++N S  +   G V      DK    RD++ +++E K  A  RL EL+
Sbjct: 241 LQ-----TGGGSLMNTSAPNGVNGSVSTDKSSDKGMGWRDLKDAVEEAKTLAANRLFELH 300

Query: 338 SLHEGRLKMLQQLSDIQNTMKSVKSISSSQSYLLLRDRIEKLKLEVYEQQALFEKLQVEK 397
              E  L + +QL DIQ+ +K    I +S+ Y +L D++  L  E+   + L E LQ EK
Sbjct: 301 ETQEDNLILSKQLEDIQDQLKDENYIVTSKPYTILSDQLHHLNAEIERYRGLVEVLQNEK 360

Query: 398 DNIIWREKELNIKNNILDMLRRSSTVSDTRIDDLEMLIQKQKDEKKSMENKLAEVLKEPG 457
           D ++ +E+E+  K   +D +++S T    +I+DLE  IQK   EK  +E K  E L++ G
Sbjct: 361 DQLMQKEEEMLAKAESVDAVQQSITTYKAKIEDLEHEIQKLMAEKNDLEIKAEEALQDSG 420

Query: 458 RKKIISEFRALVSSFPEAMGSMQSQLHKYKEAASDVHSVRADMQSLSSIIDRMEKECESL 517
           +K    E   + +S  + M  + +Q+++ K+AAS+  ++R +   L +++ +   E + +
Sbjct: 421 KKDFKDEIHVMAASLSKEMELLDNQMNRSKDAASEALALREEADYLRTLLAKKIDEQKEI 480

Query: 518 SSRSKDQIAEIQKLQATVQDLTEVNRELKLIIDMYRRESTESREVLEARDLEYKAWAHVQ 577
           S R   Q+ EI+ L+A ++ L +  +EL+ I+DM  +E +ESR + E  + E +A    +
Sbjct: 481 SDRYNTQVTEIKSLKALIETLDQEKQELQFIVDMLGKECSESRAISEIEESENRARKQAE 540

Query: 578 SLKSSLDERNLESRVKIANEAEAISQQRLAAAEAEIARLRQKLEASKRDLTRLTDALKSK 637
            L+  L+E NLE RVK ANEAE   QQRL+ AEAE+  LR K++AS+RD+ +L ++++ K
Sbjct: 541 YLRKCLEEHNLELRVKAANEAETACQQRLSIAEAELEDLRAKVDASERDVMKLKESIRIK 600

Query: 638 GDENVAYLSEIETIGQAYDDMQTQNQHLLQQITERDDYNIKLVLEGVRARQMQEIMLIEK 697
             E   ++SEIETIGQAY+DMQTQNQHLLQQ+ +RDD+NIKLV + V+ +Q    +L EK
Sbjct: 601 EAEVDGHISEIETIGQAYEDMQTQNQHLLQQVADRDDFNIKLVSDSVKMKQAYGSLLAEK 660

Query: 698 QTLEKEIQQANASLVLYEMKAARIEDQLRGCSDHIQKIEDNKLQDTDTLENSQKRLLDIR 757
             L+K++Q  N+SL   ++K    E+Q++       K          +LE +   + D  
Sbjct: 661 NMLQKQLQHVNSSLESSKLKITSGEEQMKTYVAQAMKSSSENRHLAISLERTMLEVSDAE 720

Query: 758 IASQQTRESLDQSQSKVEKCRTAQAELQIELEKERFEKKRIEEELEVVGRKASRLEAQME 817
              +  R +   ++ + E  +   AEL++ELE+ER E+ ++EEE E V  + S L ++ E
Sbjct: 721 KELKWLRSATGSAEKEYEINQKKIAELKMELERERNERIKLEEEYEEVKNEVSELTSETE 780

Query: 818 SSSVVEKLHEELGEYEKIVNCKICVNSRKQESLKK 841
            ++ ++KL +E+ E + I+ C +C +  K+  + K
Sbjct: 781 ETT-IQKLQDEIKECKAILKCGVCFDRPKEVVITK 806

BLAST of Lag0020596 vs. ExPASy TrEMBL
Match: A0A5A7U3M3 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G00490 PE=3 SV=1)

HSP 1 Score: 1406.7 bits (3640), Expect = 0.0e+00
Identity = 802/970 (82.68%), Postives = 846/970 (87.22%), Query Frame = 0

Query: 1    MGSTVDSDRKRRHFSTISPTAATAKKTPFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE 60
            MGSTV+SDRKRRHFSTISPTAATAKK PFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE
Sbjct: 506  MGSTVESDRKRRHFSTISPTAATAKKAPFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE 565

Query: 61   FKSLQNKYAQLKEKQEPYDMTVAVVKNCWEELVNGLETSSVRMRRWRSKQDGEHTMAAAD 120
            +KSLQNKYAQLKEKQEPYD TVAVVKNCWEELVNGLETSSVRMRRWRSK+DGEHT+AA D
Sbjct: 566  YKSLQNKYAQLKEKQEPYDTTVAVVKNCWEELVNGLETSSVRMRRWRSKRDGEHTIAAVD 625

Query: 121  ESSSSFEDAVLSRLAETGATQSSSAYSSNEHMEEETESPSEKTKTIERNIEASIENLWYL 180
             SSSSFEDAVLSRLAETGATQSSSAYSS++HMEEETESP EKTKTIERNIE SIENLWYL
Sbjct: 626  GSSSSFEDAVLSRLAETGATQSSSAYSSSKHMEEETESPCEKTKTIERNIETSIENLWYL 685

Query: 181  KDGLHAALLNELPKDDSFRNRAFGDLVKEVRNMRLRVKDFLFKQKGLAIELQNHRDLDAK 240
            KDGLHA LL+ELPKDDSFRNR  GDLVKEVRNMRLRVKDFLFKQK LA EL+ HRDLDAK
Sbjct: 686  KDGLHATLLSELPKDDSFRNRTSGDLVKEVRNMRLRVKDFLFKQKVLAKELEKHRDLDAK 745

Query: 241  TKAELKVLKAELESAVAELEESNSKLTKLRAEHDAAKRDGFPVLNLSGKHSGGGKVRDKQ 300
            TKAELKVLK ELESAVAELEESNSKLTKLRAEHDAAK+ GFPVLNL+GKHS  GKVRDKQ
Sbjct: 746  TKAELKVLKVELESAVAELEESNSKLTKLRAEHDAAKKAGFPVLNLAGKHSASGKVRDKQ 805

Query: 301  KDLRDMESSLKELKDQAVGRLAELNSLHEGRLKMLQQLSDIQNTMKSVKSISSSQSYLLL 360
            KDLRDME SLKELKDQAV RLAELNSLHEGRLKML++LSDIQNTMKSVKSISSS+ YLLL
Sbjct: 806  KDLRDMEFSLKELKDQAVDRLAELNSLHEGRLKMLRRLSDIQNTMKSVKSISSSKPYLLL 865

Query: 361  RDRIEKLKLEVYEQQALFEKLQ-VEKDNIIWREKELNIKNNILDMLRRSSTVSDTRIDDL 420
            RDRIEKLK EV EQQALFEKLQ VEKDNIIW+EKELNIKNNILD+LRRSSTVSDTRI+DL
Sbjct: 866  RDRIEKLKSEVNEQQALFEKLQVVEKDNIIWKEKELNIKNNILDVLRRSSTVSDTRINDL 925

Query: 421  EMLIQKQKDEKKSMENKLAEVLKEPGRKKIISEFRALVSSFPEAMGSMQSQLHKYKEAAS 480
            E+LIQKQKD KKS+ENKL EVLKEPGRKKI+SEFRALVSSFPEAMGSMQSQLHKYKEAAS
Sbjct: 926  EILIQKQKDGKKSIENKLVEVLKEPGRKKIVSEFRALVSSFPEAMGSMQSQLHKYKEAAS 985

Query: 481  DVHSVRADMQSLSSIIDRMEKECESLSSRSKDQIAEIQKLQATVQDLTEVNRELKLIIDM 540
            DVHSVRADMQSLSSIIDRMEKECESLSSRSKDQ AEIQKLQATVQDLTEVNRELKLIIDM
Sbjct: 986  DVHSVRADMQSLSSIIDRMEKECESLSSRSKDQHAEIQKLQATVQDLTEVNRELKLIIDM 1045

Query: 541  YRRESTESREVLEARDLEYKAWAHVQSLKSSLDERNLESRVKIANEAEAISQQRLAAAEA 600
            Y RESTESREVLEARDLEYKAWA VQSLKSSLDERNLESRVK ANE+EAISQQRLAAAEA
Sbjct: 1046 YSRESTESREVLEARDLEYKAWARVQSLKSSLDERNLESRVKTANESEAISQQRLAAAEA 1105

Query: 601  EIARLRQKLEASKRDLTRLTDALKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITE 660
            EIARLRQKLEASKRDLTRL+D LKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITE
Sbjct: 1106 EIARLRQKLEASKRDLTRLSDVLKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITE 1165

Query: 661  RDDYNIKLVLEGVRARQMQEIMLIEKQTLEKEIQQANASLVLYEMKAARIEDQLRGCSDH 720
            RDDYNIKLVLEGVRARQ+QEIMLIEKQ LE E+QQ NASLVLYEMKAARIEDQLRGCSD 
Sbjct: 1166 RDDYNIKLVLEGVRARQLQEIMLIEKQALENEVQQGNASLVLYEMKAARIEDQLRGCSDQ 1225

Query: 721  IQKIEDNKLQDTDTLENSQKRLLDIRIASQQTRESLDQSQSKVEKCRTAQAELQIELEKE 780
            IQKIE++KL+DTDTLEN+QKRLL+IRIASQQTRESLDQS  KVEK RT QAELQIELEKE
Sbjct: 1226 IQKIEEDKLRDTDTLENTQKRLLEIRIASQQTRESLDQSHYKVEKSRTTQAELQIELEKE 1285

Query: 781  RFEKKRIEEELEVVGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQESLK 840
            RFEKKRIEEELEV+GRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQ  + 
Sbjct: 1286 RFEKKRIEEELEVIGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQVVIT 1345

Query: 841  KGPLYNERKSYKKTLQGSRKRKTPLPSEMEESPNKAKRSLISNASRSYQIGSDEVKGNGR 900
            K   +       + +  S+ RK P             RS                     
Sbjct: 1346 K-CFHLFCNPCVQDILKSQHRKCP-------------RSAF------------------- 1405

Query: 901  GAHGSRKYGCHRMKFQGCHYKVLPLVLLIRIASLLNTHGCGWTQTNKLYFAQPKNSTPTP 960
            GAH    +  H + +   + +++P    + I          W   +K YFAQP +  PTP
Sbjct: 1406 GAHFMAIWLRHELTWG--YKQIIPNCFTLLIQP--------WMDIDKAYFAQPNSCAPTP 1432

Query: 961  TVEARVDVWC 970
            TVEA VDVWC
Sbjct: 1466 TVEATVDVWC 1432

BLAST of Lag0020596 vs. ExPASy TrEMBL
Match: A0A1S3AVH9 (E3 ubiquitin protein ligase OS=Cucumis melo OX=3656 GN=LOC103483129 PE=3 SV=1)

HSP 1 Score: 1395.6 bits (3611), Expect = 0.0e+00
Identity = 771/840 (91.79%), Postives = 801/840 (95.36%), Query Frame = 0

Query: 1   MGSTVDSDRKRRHFSTISPTAATAKKTPFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE 60
           MGSTV+SDRKRRHFSTISPTAATAKK PFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE
Sbjct: 1   MGSTVESDRKRRHFSTISPTAATAKKAPFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE 60

Query: 61  FKSLQNKYAQLKEKQEPYDMTVAVVKNCWEELVNGLETSSVRMRRWRSKQDGEHTMAAAD 120
           +KSLQNKYAQLKEKQEPYD TVAVVKNCWEELVNGLETSSVRMRRWRSK+DGEHT+AA D
Sbjct: 61  YKSLQNKYAQLKEKQEPYDTTVAVVKNCWEELVNGLETSSVRMRRWRSKRDGEHTIAAVD 120

Query: 121 ESSSSFEDAVLSRLAETGATQSSSAYSSNEHMEEETESPSEKTKTIERNIEASIENLWYL 180
            SSSSFEDAVLSRLAETGATQSSSAYSS++HMEEETESP EKTKTIERNIE SIENLWYL
Sbjct: 121 GSSSSFEDAVLSRLAETGATQSSSAYSSSKHMEEETESPCEKTKTIERNIETSIENLWYL 180

Query: 181 KDGLHAALLNELPKDDSFRNRAFGDLVKEVRNMRLRVKDFLFKQKGLAIELQNHRDLDAK 240
           KDGLHA LL+ELPKDDSFRNR  GDLVKEVRNMRLRVKDFLFKQK LA EL+ HRDLDAK
Sbjct: 181 KDGLHATLLSELPKDDSFRNRTSGDLVKEVRNMRLRVKDFLFKQKVLAKELEKHRDLDAK 240

Query: 241 TKAELKVLKAELESAVAELEESNSKLTKLRAEHDAAKRDGFPVLNLSGKHSGGGKVRDKQ 300
           TKAELKVLK ELESAVAELEESNSKLTKLRAEHDAAK+ GFPVLNL+GKHS  GKVRDKQ
Sbjct: 241 TKAELKVLKVELESAVAELEESNSKLTKLRAEHDAAKKAGFPVLNLAGKHSASGKVRDKQ 300

Query: 301 KDLRDMESSLKELKDQAVGRLAELNSLHEGRLKMLQQLSDIQNTMKSVKSISSSQSYLLL 360
           KDLRDME SLKELKDQAV RLAELNSLHEGRLKML++LSDIQNTMKSVKSISSS+ YLLL
Sbjct: 301 KDLRDMEFSLKELKDQAVDRLAELNSLHEGRLKMLRRLSDIQNTMKSVKSISSSKPYLLL 360

Query: 361 RDRIEKLKLEVYEQQALFEKLQVEKDNIIWREKELNIKNNILDMLRRSSTVSDTRIDDLE 420
           RDRIEKLK EV EQQALFEKLQVEKDNIIW+EKELNIKNNILD+LRRSSTVSDTRI+DLE
Sbjct: 361 RDRIEKLKSEVNEQQALFEKLQVEKDNIIWKEKELNIKNNILDVLRRSSTVSDTRINDLE 420

Query: 421 MLIQKQKDEKKSMENKLAEVLKEPGRKKIISEFRALVSSFPEAMGSMQSQLHKYKEAASD 480
           +LIQKQKD KKS+ENKL EVLKEPGRKKI+SEFRALVSSFPEAMGSMQSQLHKYKEAASD
Sbjct: 421 ILIQKQKDGKKSIENKLVEVLKEPGRKKIVSEFRALVSSFPEAMGSMQSQLHKYKEAASD 480

Query: 481 VHSVRADMQSLSSIIDRMEKECESLSSRSKDQIAEIQKLQATVQDLTEVNRELKLIIDMY 540
           VHSVRADMQSLSSIIDRMEKECESLSSRSKDQ AEIQKLQATVQDLTEVNRELKLIIDMY
Sbjct: 481 VHSVRADMQSLSSIIDRMEKECESLSSRSKDQHAEIQKLQATVQDLTEVNRELKLIIDMY 540

Query: 541 RRESTESREVLEARDLEYKAWAHVQSLKSSLDERNLESRVKIANEAEAISQQRLAAAEAE 600
            RESTESREVLEARDLEYKAWA VQSLKSSLDERNLESRVK ANE+EAISQQRLAAAEAE
Sbjct: 541 SRESTESREVLEARDLEYKAWARVQSLKSSLDERNLESRVKTANESEAISQQRLAAAEAE 600

Query: 601 IARLRQKLEASKRDLTRLTDALKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITER 660
           IARLRQKLEASKRDLTRL+D LKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITER
Sbjct: 601 IARLRQKLEASKRDLTRLSDVLKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITER 660

Query: 661 DDYNIKLVLEGVRARQMQEIMLIEKQTLEKEIQQANASLVLYEMKAARIEDQLRGCSDHI 720
           DDYNIKLVLEGVRARQ+QEIMLIEKQ LE E+QQ NASLVLYEMKAARIEDQLRGCSD I
Sbjct: 661 DDYNIKLVLEGVRARQLQEIMLIEKQALENEVQQGNASLVLYEMKAARIEDQLRGCSDQI 720

Query: 721 QKIEDNKLQDTDTLENSQKRLLDIRIASQQTRESLDQSQSKVEKCRTAQAELQIELEKER 780
           QKIE++KL+DTDTLEN+QKRLL+IRIASQQTRESLDQS  KVEK RT QAELQIELEKER
Sbjct: 721 QKIEEDKLRDTDTLENTQKRLLEIRIASQQTRESLDQSHYKVEKSRTTQAELQIELEKER 780

Query: 781 FEKKRIEEELEVVGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQESLKK 840
           FEKKRIEEELEV+GRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQ  + K
Sbjct: 781 FEKKRIEEELEVIGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQVVITK 840

BLAST of Lag0020596 vs. ExPASy TrEMBL
Match: A0A0A0L2Z2 (E3 ubiquitin protein ligase OS=Cucumis sativus OX=3659 GN=Csa_3G118000 PE=3 SV=1)

HSP 1 Score: 1389.4 bits (3595), Expect = 0.0e+00
Identity = 763/840 (90.83%), Postives = 802/840 (95.48%), Query Frame = 0

Query: 1   MGSTVDSDRKRRHFSTISPTAATAKKTPFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE 60
           MGSTV+SDRKRRHFSTISPTAATAKK PFLP+SEDKKLDVAVLQYQNQKLIQKLEVQKVE
Sbjct: 1   MGSTVESDRKRRHFSTISPTAATAKKAPFLPVSEDKKLDVAVLQYQNQKLIQKLEVQKVE 60

Query: 61  FKSLQNKYAQLKEKQEPYDMTVAVVKNCWEELVNGLETSSVRMRRWRSKQDGEHTMAAAD 120
           +KSLQNKYAQLKEKQEPYD TVAVVKNCWEELVNGLETSSVRMRRWRSK+DGEHT+A  D
Sbjct: 61  YKSLQNKYAQLKEKQEPYDTTVAVVKNCWEELVNGLETSSVRMRRWRSKRDGEHTIAGVD 120

Query: 121 ESSSSFEDAVLSRLAETGATQSSSAYSSNEHMEEETESPSEKTKTIERNIEASIENLWYL 180
            SSSSFEDAVLSRLAETGATQSSS YSS++HMEEETESP EKTKTIER+IE SIENLWYL
Sbjct: 121 GSSSSFEDAVLSRLAETGATQSSSTYSSSKHMEEETESPCEKTKTIERSIETSIENLWYL 180

Query: 181 KDGLHAALLNELPKDDSFRNRAFGDLVKEVRNMRLRVKDFLFKQKGLAIELQNHRDLDAK 240
           KDGLHA LLNELPKDDSFR R  GDLVKEVRNMRLRVKDFLFKQK LA EL+ HRDLDAK
Sbjct: 181 KDGLHATLLNELPKDDSFRKRTSGDLVKEVRNMRLRVKDFLFKQKVLAQELEKHRDLDAK 240

Query: 241 TKAELKVLKAELESAVAELEESNSKLTKLRAEHDAAKRDGFPVLNLSGKHSGGGKVRDKQ 300
           TKAELKVLK EL SAVAELEESNSKLTKLRAEHDAAK+ GFPVLNL+GKHS  GKVRDKQ
Sbjct: 241 TKAELKVLKVELGSAVAELEESNSKLTKLRAEHDAAKKAGFPVLNLTGKHSASGKVRDKQ 300

Query: 301 KDLRDMESSLKELKDQAVGRLAELNSLHEGRLKMLQQLSDIQNTMKSVKSISSSQSYLLL 360
           KDLRDMESSLKELKDQAV RLAELNSLHEGRLKML++LSDIQNTMKSVK+ISSS+ YLLL
Sbjct: 301 KDLRDMESSLKELKDQAVDRLAELNSLHEGRLKMLRRLSDIQNTMKSVKTISSSKPYLLL 360

Query: 361 RDRIEKLKLEVYEQQALFEKLQVEKDNIIWREKELNIKNNILDMLRRSSTVSDTRIDDLE 420
           RDRIEKLKLEV EQQALFEKLQVEKDNI+W+EKELNIKNNILD+LRRSSTVSDTRI+DLE
Sbjct: 361 RDRIEKLKLEVNEQQALFEKLQVEKDNIMWKEKELNIKNNILDVLRRSSTVSDTRINDLE 420

Query: 421 MLIQKQKDEKKSMENKLAEVLKEPGRKKIISEFRALVSSFPEAMGSMQSQLHKYKEAASD 480
           +LIQKQKD K+S+ENKL EVLKEPGRKKI+SEFRALVSSFPEAMGSMQSQLHKYKEAASD
Sbjct: 421 ILIQKQKDGKQSIENKLVEVLKEPGRKKIVSEFRALVSSFPEAMGSMQSQLHKYKEAASD 480

Query: 481 VHSVRADMQSLSSIIDRMEKECESLSSRSKDQIAEIQKLQATVQDLTEVNRELKLIIDMY 540
           VHSVRAD+QSLSSIIDRMEKECE+LSSRSKDQ AEIQKLQATVQDLTEVNRELKLIIDMY
Sbjct: 481 VHSVRADLQSLSSIIDRMEKECENLSSRSKDQQAEIQKLQATVQDLTEVNRELKLIIDMY 540

Query: 541 RRESTESREVLEARDLEYKAWAHVQSLKSSLDERNLESRVKIANEAEAISQQRLAAAEAE 600
            RESTESREVLEARDLEYKAWA VQSLKSSLDERNLESRVK ANEAEAISQQRLAAAEAE
Sbjct: 541 SRESTESREVLEARDLEYKAWARVQSLKSSLDERNLESRVKTANEAEAISQQRLAAAEAE 600

Query: 601 IARLRQKLEASKRDLTRLTDALKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITER 660
           IARLRQKLEASKRDLTRL+D LKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITER
Sbjct: 601 IARLRQKLEASKRDLTRLSDVLKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITER 660

Query: 661 DDYNIKLVLEGVRARQMQEIMLIEKQTLEKEIQQANASLVLYEMKAARIEDQLRGCSDHI 720
           DDYNIKLVLEGVRARQ+QEIMLIEKQ LE E+QQANASLVLYEMKAARIEDQLRGCSDHI
Sbjct: 661 DDYNIKLVLEGVRARQLQEIMLIEKQALENEVQQANASLVLYEMKAARIEDQLRGCSDHI 720

Query: 721 QKIEDNKLQDTDTLENSQKRLLDIRIASQQTRESLDQSQSKVEKCRTAQAELQIELEKER 780
           QKIE++KL+DTDTLEN++KRLL+IRIASQQTRESLD+ QSKVE+ RT QAELQIELEKER
Sbjct: 721 QKIEEDKLRDTDTLENTRKRLLEIRIASQQTRESLDECQSKVERSRTTQAELQIELEKER 780

Query: 781 FEKKRIEEELEVVGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQESLKK 840
           FEKKRIEEELEV+GRKASRLEAQMESSSV+EKLHEELGEYEKIVNCKICVNSRKQ  + K
Sbjct: 781 FEKKRIEEELEVIGRKASRLEAQMESSSVIEKLHEELGEYEKIVNCKICVNSRKQVVITK 840

BLAST of Lag0020596 vs. ExPASy TrEMBL
Match: A0A5D3DB61 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G00490 PE=3 SV=1)

HSP 1 Score: 1382.5 bits (3577), Expect = 0.0e+00
Identity = 802/1022 (78.47%), Postives = 846/1022 (82.78%), Query Frame = 0

Query: 1    MGSTVDSDRKRRHFSTISPTAATAKKTPFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE 60
            MGSTV+SDRKRRHFSTISPTAATAKK PFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE
Sbjct: 506  MGSTVESDRKRRHFSTISPTAATAKKAPFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE 565

Query: 61   FKSLQNKYAQLKEKQEPYDMTVAVVKNCWEELVNGLETSSVRMRRWRSKQDGEHTMAAAD 120
            +KSLQNKYAQLKEKQEPYD TVAVVKNCWEELVNGLETSSVRMRRWRSK+DGEHT+AA D
Sbjct: 566  YKSLQNKYAQLKEKQEPYDTTVAVVKNCWEELVNGLETSSVRMRRWRSKRDGEHTIAAVD 625

Query: 121  ESSSSFEDAVLSRLAETGATQSSSAYSSNEHMEEETESPSEKTKTIERNIEASIENLWYL 180
             SSSSFEDAVLSRLAETGATQSSSAYSS++HMEEETESP EKTKTIERNIE SIENLWYL
Sbjct: 626  GSSSSFEDAVLSRLAETGATQSSSAYSSSKHMEEETESPCEKTKTIERNIETSIENLWYL 685

Query: 181  KDGLHAALLNELPKDDSFRNRAFGDLVKEVRNMRLRVKDFLFKQKGLAIELQNHRDLDAK 240
            KDGLHA LL+ELPKDDSFRNR  GDLVKEVRNMRLRVKDFLFKQK LA EL+ HRDLDAK
Sbjct: 686  KDGLHATLLSELPKDDSFRNRTSGDLVKEVRNMRLRVKDFLFKQKVLAKELEKHRDLDAK 745

Query: 241  TKAELKVLK----------------------AELESAVAELEESNSKLTKLRAEHDAAKR 300
            TKAELKVLK                       ELESAVAELEESNSKLTKLRAEHDAAK+
Sbjct: 746  TKAELKVLKGAPSILSMVGSLLVLMHYHNYFVELESAVAELEESNSKLTKLRAEHDAAKK 805

Query: 301  DGFPVLNLSGKHSGGGKVRDKQKDLRDMESSLKELKDQAVGRLAELNSLHEGRLKMLQQL 360
             GFPVLNL+GKHS  GKVRDKQKDLRDME SLKELKDQAV RLAELNSLHEGRLKML++L
Sbjct: 806  AGFPVLNLAGKHSASGKVRDKQKDLRDMEFSLKELKDQAVDRLAELNSLHEGRLKMLRRL 865

Query: 361  SDIQNTMKSVKSISSSQSYLLLRDRIEKLKLEVYEQQALFEKL----------------- 420
            SDIQNTMKSVKSISSS+ YLLLRDRIEKLK EV EQQALFEKL                 
Sbjct: 866  SDIQNTMKSVKSISSSKPYLLLRDRIEKLKSEVNEQQALFEKLQVVNWSVVALMPESVLY 925

Query: 421  --------------QVEKDNIIWREKELNIKNNILDMLRRSSTVSDTRIDDLEMLIQKQK 480
                          QVEKDNIIW+EKELNIKNNILD+LRRSSTVSDTRI+DLE+LIQKQK
Sbjct: 926  YVLRFFDANTCCQHQVEKDNIIWKEKELNIKNNILDVLRRSSTVSDTRINDLEILIQKQK 985

Query: 481  DEKKSMENKLAEVLKEPGRKKIISEFRALVSSFPEAMGSMQSQLHKYKEAASDVHSVRAD 540
            D KKS+ENKL EVLKEPGRKKI+SEFRALVSSFPEAMGSMQSQLHKYKEAASDVHSVRAD
Sbjct: 986  DGKKSIENKLVEVLKEPGRKKIVSEFRALVSSFPEAMGSMQSQLHKYKEAASDVHSVRAD 1045

Query: 541  MQSLSSIIDRMEKECESLSSRSKDQIAEIQKLQATVQDLTEVNRELKLIIDMYRRESTES 600
            MQSLSSIIDRMEKECESLSSRSKDQ AEIQKLQATVQDLTEVNRELKLIIDMY RESTES
Sbjct: 1046 MQSLSSIIDRMEKECESLSSRSKDQHAEIQKLQATVQDLTEVNRELKLIIDMYSRESTES 1105

Query: 601  REVLEARDLEYKAWAHVQSLKSSLDERNLESRVKIANEAEAISQQRLAAAEAEIARLRQK 660
            REVLEARDLEYKAWA VQSLKSSLDERNLESRVK ANE+EAISQQRLAAAEAEIARLRQK
Sbjct: 1106 REVLEARDLEYKAWARVQSLKSSLDERNLESRVKTANESEAISQQRLAAAEAEIARLRQK 1165

Query: 661  LEASKRDLTRLTDALKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITERDDYNIKL 720
            LEASKRDLTRL+D LKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITERDDYNIKL
Sbjct: 1166 LEASKRDLTRLSDVLKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITERDDYNIKL 1225

Query: 721  VLEGVRARQMQEIMLIEKQTLEKEIQQANASLVLYEMKAARIEDQLRGCSDHIQKIEDNK 780
            VLEGVRARQ+QEIMLIEKQ LE E+QQ NASLVLYEMKAARIEDQLRGCSD IQKIE++K
Sbjct: 1226 VLEGVRARQLQEIMLIEKQALENEVQQGNASLVLYEMKAARIEDQLRGCSDQIQKIEEDK 1285

Query: 781  LQDTDTLENSQKRLLDIRIASQQTRESLDQSQSKVEKCRTAQAELQIELEKERFEKKRIE 840
            L+DTDTLEN+QKRLL+IRIASQQTRESLDQS  KVEK RT QAELQIELEKERFEKKRIE
Sbjct: 1286 LRDTDTLENTQKRLLEIRIASQQTRESLDQSHYKVEKSRTTQAELQIELEKERFEKKRIE 1345

Query: 841  EELEVVGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQESLKKGPLYNER 900
            EELEV+GRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQ  + K   +   
Sbjct: 1346 EELEVIGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQVVITK-CFHLFC 1405

Query: 901  KSYKKTLQGSRKRKTPLPSEMEESPNKAKRSLISNASRSYQIGSDEVKGNGRGAHGSRKY 960
                + +  S+ RK P             RS                     GAH    +
Sbjct: 1406 NPCVQDILKSQHRKCP-------------RSAF-------------------GAHFMAIW 1465

Query: 961  GCHRMKFQGCHYKVLPLVLLIRIASLLNTHGCGWTQTNKLYFAQPKNSTPTPTVEARVDV 970
              H + +   + +++P    + I          W   +K YFAQP +  PTPTVEA VDV
Sbjct: 1466 LRHELTWG--YKQIIPNCFTLLIQP--------WMDIDKAYFAQPNSCAPTPTVEATVDV 1484

BLAST of Lag0020596 vs. ExPASy TrEMBL
Match: A0A6J1E9A6 (E3 ubiquitin protein ligase OS=Cucurbita moschata OX=3662 GN=LOC111431961 PE=3 SV=1)

HSP 1 Score: 1382.5 bits (3577), Expect = 0.0e+00
Identity = 762/840 (90.71%), Postives = 796/840 (94.76%), Query Frame = 0

Query: 1   MGSTVDSDRKRRHFSTISPTAATAKKTPFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE 60
           MGSTV+SDRKRRHFSTISPTAATAKK PFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE
Sbjct: 1   MGSTVESDRKRRHFSTISPTAATAKKAPFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVE 60

Query: 61  FKSLQNKYAQLKEKQEPYDMTVAVVKNCWEELVNGLETSSVRMRRWRSKQDGEHTMAAAD 120
           +KSLQNKYA LKEKQEPYDMTVAVVKNCWEELVNGLE SSV MRRWRSKQDGE ++AA D
Sbjct: 61  YKSLQNKYAHLKEKQEPYDMTVAVVKNCWEELVNGLEKSSVCMRRWRSKQDGEQSIAAVD 120

Query: 121 ESSSSFEDAVLSRLAETGATQSSSAYSSNEHMEEETESPSEKTKTIERNIEASIENLWYL 180
            SSSSFE+AVLSRLAETGATQSSS+YSS +HMEEETESP EKTKTIERNIEASIENLWYL
Sbjct: 121 GSSSSFENAVLSRLAETGATQSSSSYSSTKHMEEETESPCEKTKTIERNIEASIENLWYL 180

Query: 181 KDGLHAALLNELPKDDSFRNRAFGDLVKEVRNMRLRVKDFLFKQKGLAIELQNHRDLDAK 240
           KDGLHA LL+ELP DDSFRNR  GDLVKEVRNMRLRVKDFLFKQK LA ELQNHRDLDAK
Sbjct: 181 KDGLHATLLSELPIDDSFRNRTSGDLVKEVRNMRLRVKDFLFKQKVLAKELQNHRDLDAK 240

Query: 241 TKAELKVLKAELESAVAELEESNSKLTKLRAEHDAAKRDGFPVLNLSGKHSGGGKVRDKQ 300
           TKAELKVLK ELESAVAELE+SNSKLTKLRAEHDAAK+ GFPVLNL+GKHS   KVRDKQ
Sbjct: 241 TKAELKVLKVELESAVAELEDSNSKLTKLRAEHDAAKKAGFPVLNLAGKHSSSSKVRDKQ 300

Query: 301 KDLRDMESSLKELKDQAVGRLAELNSLHEGRLKMLQQLSDIQNTMKSVKSISSSQSYLLL 360
           KDLRDMES+LKELKDQAV RLAELNSLHEGRLKMLQQLSD QN MKSVK +S+SQSYLLL
Sbjct: 301 KDLRDMESALKELKDQAVDRLAELNSLHEGRLKMLQQLSDFQNAMKSVKCLSASQSYLLL 360

Query: 361 RDRIEKLKLEVYEQQALFEKLQVEKDNIIWREKELNIKNNILDMLRRSSTVSDTRIDDLE 420
           RDRIEKLK EVYEQQALFEKLQVEKDNIIWREKELN+KNNILD+LRRSSTVSDT+I+DLE
Sbjct: 361 RDRIEKLKSEVYEQQALFEKLQVEKDNIIWREKELNVKNNILDVLRRSSTVSDTKINDLE 420

Query: 421 MLIQKQKDEKKSMENKLAEVLKEPGRKKIISEFRALVSSFPEAMGSMQSQLHKYKEAASD 480
           MLIQK KDEK S ENKL EVLKEPGRKKI+SEFRALVSSFPEAMGSMQ+QLHKYKEAASD
Sbjct: 421 MLIQKHKDEKMSTENKLVEVLKEPGRKKIVSEFRALVSSFPEAMGSMQNQLHKYKEAASD 480

Query: 481 VHSVRADMQSLSSIIDRMEKECESLSSRSKDQIAEIQKLQATVQDLTEVNRELKLIIDMY 540
           VHSVRA MQSLSSIIDRMEKECE LSS+SKDQIAEIQKLQATVQDLTE NRELKLIIDMY
Sbjct: 481 VHSVRAFMQSLSSIIDRMEKECEGLSSQSKDQIAEIQKLQATVQDLTEFNRELKLIIDMY 540

Query: 541 RRESTESREVLEARDLEYKAWAHVQSLKSSLDERNLESRVKIANEAEAISQQRLAAAEAE 600
           RRESTESRE LEARDLEYKAWA VQSLKSSLDERNLESRVK ANEAEAISQQRLAAAEAE
Sbjct: 541 RRESTESREALEARDLEYKAWARVQSLKSSLDERNLESRVKTANEAEAISQQRLAAAEAE 600

Query: 601 IARLRQKLEASKRDLTRLTDALKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITER 660
           IARLRQKLEASKRDLTRL+D LKSK DENVAYLSEIETIGQAYDDMQTQNQHLLQQITER
Sbjct: 601 IARLRQKLEASKRDLTRLSDVLKSKSDENVAYLSEIETIGQAYDDMQTQNQHLLQQITER 660

Query: 661 DDYNIKLVLEGVRARQMQEIMLIEKQTLEKEIQQANASLVLYEMKAARIEDQLRGCSDHI 720
           DDYNIKLVLEGVRARQ+QEIMLIEKQTLEKEIQQANASLVL++MKAAR+EDQLRGCSDHI
Sbjct: 661 DDYNIKLVLEGVRARQLQEIMLIEKQTLEKEIQQANASLVLFDMKAARVEDQLRGCSDHI 720

Query: 721 QKIEDNKLQDTDTLENSQKRLLDIRIASQQTRESLDQSQSKVEKCRTAQAELQIELEKER 780
           +KI+D+KL DTDTLE+ QKRLLDI++ASQQTRESLDQSQSKVEKCRT QAELQIELEKER
Sbjct: 721 KKIKDDKLHDTDTLEHIQKRLLDIKLASQQTRESLDQSQSKVEKCRTTQAELQIELEKER 780

Query: 781 FEKKRIEEELEVVGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQESLKK 840
           FEKKRIEEELEVV RKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICV+SRKQ  + K
Sbjct: 781 FEKKRIEEELEVVARKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVDSRKQVVITK 840

BLAST of Lag0020596 vs. TAIR 10
Match: AT2G44950.1 (histone mono-ubiquitination 1 )

HSP 1 Score: 693.7 bits (1789), Expect = 2.1e-199
Identity = 422/866 (48.73%), Postives = 589/866 (68.01%), Query Frame = 0

Query: 1   MGSTVDSDRKRRHFSTISPT--AATAKKTPFLPISEDKKLDVAVLQYQNQKLIQKLEVQK 60
           M ST + DRKRRHFS+ISP+  AA  KK PF   S + KLD AVLQ+QN KL QKLE Q+
Sbjct: 1   MASTGEPDRKRRHFSSISPSEAAAAVKKQPFFWPSSEDKLDTAVLQFQNLKLSQKLEAQQ 60

Query: 61  VEFKSLQNKYAQLKEKQEPYDMTVAVVKNCWEELVNGLETSSVRMRRWRSKQDGEHTMA- 120
           VE   L++K +Q+KEKQ PY+ ++  V   WE+L   +E+ SVR+        G H    
Sbjct: 61  VECSILEDKLSQIKEKQLPYNSSLKTVHKSWEKLTASVESCSVRV---SDSSSGAHRFVN 120

Query: 121 AADESSSSFEDAVLSRLAETGATQSSSAYSSNEHMEEETESPSEKTKTIERNIEASIENL 180
             D SS + ++  ++RL ETGAT+SSS+   +  MEE   + S +      N+ A+ E+L
Sbjct: 121 KEDGSSPAVKNDFINRLLETGATESSSSNICSNQMEENGVNTSSQMTQTLYNLVAATEDL 180

Query: 181 WYLKDGLHAALLNELPKDDSFRNRAFGDLVKEVRNMRLRVKDFLFKQKGLAIELQNHRDL 240
             LKD L+  +L      D     A  +L  E+++ R  + D L K K L+ ELQ+HRD 
Sbjct: 181 RCLKDELYPTVLRTNLGKDLCGQLALSELESEIKSFRGDLDDVLVKFKSLSRELQSHRDA 240

Query: 241 DAKTKAELKVLKAELESAVAELEESNSKLTKLRAEHDAAKRDGFPVLNLSGKHSGGGKVR 300
           DAK + +LK ++ ELE  V EL++ N  L+ LRAE DA     FPVL+L  K +   + R
Sbjct: 241 DAKVRVDLKRIRGELEDEVVELQQCNGDLSALRAERDATAGAFFPVLSLGNKLATSDRER 300

Query: 301 DKQKDLRDMESSLKELKDQAVGRLAELNSLHEGRLKMLQQLSDIQNTMKSVKSISSSQSY 360
           DKQ+DL+DME+ LKEL   A GRL +L +LHE R KML ++S++QN  KSV+ ISSSQ+ 
Sbjct: 301 DKQRDLQDMETVLKELTVLASGRLQQLKNLHEERTKMLGKMSNLQNKSKSVRCISSSQAC 360

Query: 361 LLLRDRIEKLKLEVYEQQALFEKLQVEKDNIIWREKELNIKNNILDMLRRSSTVSDTRID 420
           L L+D++EK K  V++  AL EKLQVEKD+I+W+E+E+NIKN + D+ R++S V+D+R+ 
Sbjct: 361 LSLKDQLEKSKEAVFQYMALLEKLQVEKDSIVWKEREINIKNELGDVSRKTSAVTDSRMA 420

Query: 421 DLEMLIQKQKDEKKSMENKLAEVLKEPGRKKIISEFRALVSSFPEAMGSMQSQLHKYKEA 480
            L+  IQKQ DEK  ++ +L  + +E GRK+I ++ +AL+SSFPE M SM+SQL+ YKE 
Sbjct: 421 SLDSEIQKQLDEKMRIKTRLGNISRERGRKEIFADMKALISSFPEEMSSMRSQLNNYKET 480

Query: 481 ASDVHSVRADMQSLSSIIDRMEKECESLSSRSKDQIAEIQKLQATVQDLTEVNRELKLII 540
           A  +HS+RAD+QSLS ++ R  KE E+L  RS D  +++  L ATV DL   + ELKL +
Sbjct: 481 AGGIHSLRADVQSLSGVLCRKTKEYEALQLRSADYASQLGDLNATVCDLKNSHEELKLFL 540

Query: 541 DMYRRESTESREVLEARDLEYKAWAHVQSLKSSLDERNLESRVKIANEAEAISQQRLAAA 600
           DMY+REST++R++ EA++ EY+AWAHVQSLKSSLDE+NLE RVK ANEAEA+SQQ LAAA
Sbjct: 541 DMYKRESTDARDIAEAKEQEYRAWAHVQSLKSSLDEQNLELRVKAANEAEAVSQQMLAAA 600

Query: 601 EAEIARLRQKLEASKRDLTRLTDALKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQI 660
           EAEIA LRQK++  KRD+ + +D LKSK +E+  YLSEI+TIG AY+D+  QNQ LL Q+
Sbjct: 601 EAEIADLRQKMDDCKRDVAKHSDILKSKHEEHGTYLSEIQTIGSAYEDIVPQNQQLLLQV 660

Query: 661 TERDDYNIKLVLEGVRARQMQEIMLIEKQTLEKEIQQANASLVLYEMKAARIEDQLRGCS 720
           TERDDYNIKL LEG+ +RQMQ+ +LI+K  ++K+IQQ +A       K++RIEDQLR C+
Sbjct: 661 TERDDYNIKLFLEGITSRQMQDTLLIDKYIMDKDIQQGSAYASFLSKKSSRIEDQLRFCT 720

Query: 721 DHIQKIEDNKLQDTDTLENSQKRLLDIRIASQQTRESLDQSQSKVEKCRTAQAELQIELE 780
           D  QK+ ++K Q + +LEN QK+  DI    +Q R  L++S SKVE+ R     L++ELE
Sbjct: 721 DQFQKLAEDKYQKSVSLENLQKKRADIGNGLEQARSRLEESHSKVEQSRLDYGALELELE 780

Query: 781 KERFEKKRIEEELEVVGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQES 840
            ERF ++RIEEE+E+  +K SRL + +E SS ++KL +EL E+++I+ CK C N R +E 
Sbjct: 781 IERFNRRRIEEEMEIAKKKVSRLRSLIEGSSAIQKLRQELSEFKEILKCKAC-NDRPKEV 840

Query: 841 LKKGPLYNERKSYKKTLQGSRKRKTP 864
           +     +       + L G+R++K P
Sbjct: 841 VITKCYHLFCNPCVQKLTGTRQKKCP 862

BLAST of Lag0020596 vs. TAIR 10
Match: AT1G55250.3 (histone mono-ubiquitination 2 )

HSP 1 Score: 339.7 bits (870), Expect = 7.8e-93
Identity = 268/841 (31.87%), Postives = 449/841 (53.39%), Query Frame = 0

Query: 9   RKRRHFSTISPTAATAKKTPFLPISEDKKLDVAVLQYQNQKLIQKLEVQKVEFKSLQNKY 68
           +K     ++SP +     +P  PI+  K +D  VLQ QNQKL+Q+L++QK +   +++K 
Sbjct: 12  KKPHLLDSVSPNSMARNSSPSHPIA--KSVDATVLQLQNQKLVQQLDLQKKQLYDVESKI 71

Query: 69  AQLKEKQEPYDMTVAVVKNCWEELVNGLETSSVRMRRWRSKQDGEHTMAAADESSSSF-- 128
            +L+  Q  YD  +  V   W +LV+ L    VR     + Q+  + +   D+   S   
Sbjct: 72  QELQLNQTSYDDELISVNQLWNQLVDDLILLGVRA---GANQEALNYLDIVDKKRGSVPP 131

Query: 129 ---EDAVLSRLAETGATQSSSAYSSNEHMEE----ETESPSEKTKTIERNIEASIENLWY 188
              ++  L RL +  +  +S +      +EE       S  E     E  I+        
Sbjct: 132 CAADETFLCRLLQVDSLDTSKSDEVVRKVEEALALRHSSTMELMGLFENTIDTQKTKAES 191

Query: 189 LKDGLHAALLNELPKDDSFRNRAFGDLVK-EVRNMRLRVKDFLFKQKGLAIELQNHRDLD 248
           +   LHA    E   D + +  +  DL+K E +N+R  +     + K  + ++Q +    
Sbjct: 192 ISQSLHAVKSTE---DATIQLSSINDLMKEESKNLREMIDALHVRHKEHSEQIQAYISSH 251

Query: 249 AKTKAELKVLKAELESAVAELEESNSKLTKLRAEHDAAKRDGFPVLNLSGKHSGGGKVRD 308
           +  ++ELK LK +LE   AELEE+  KL  L+ + DAA         ++       K  D
Sbjct: 252 STDQSELKHLKGQLEEIKAELEENRRKLITLKMQKDAACEGHVTSPAIANGSLSPEKPVD 311

Query: 309 KQKDLRDMESSLKELKDQAVGRLAELNSLHEGRLKMLQQLSDIQNTMKSVKSISSSQSYL 368
           K K LR+++ S+ E+K  A GRL+EL +  E  L + +Q  DI+N +K  + I SS+ Y 
Sbjct: 312 KTK-LRELKDSIDEIKIMAEGRLSELQASQEYNLSLSRQCQDIENELKDDQYIYSSRLYS 371

Query: 369 LLRDRIEKLKLEVYEQQALFEKLQVEKDNIIWREKELNIKNNILDMLRRSSTVSDTRIDD 428
           L+ DRI     E+   + L E +Q E+  ++ R+KELN++   L+     +T   +RI+ 
Sbjct: 372 LINDRIHHWNAELDRYKILTEAIQAERSFVMRRDKELNLRAESLEAANHKTTTVGSRIEV 431

Query: 429 LEMLIQKQKDEKKSMENKLAEVLKEPGRKKIISEFRALVSSFPEAMGSMQSQLHKYKEAA 488
           LE  +Q    EK  +E +  E +++  R+ I SEF A+ S+  + M  M++QL ++K+ A
Sbjct: 432 LEKKLQSCIIEKNGLELETEEAIQDSERQDIKSEFIAMASTLSKEMEMMEAQLKRWKDTA 491

Query: 489 SDVHSVRADMQSL----SSIIDRMEKECESLSSRSKDQIAEIQKLQATVQDLTEVNRELK 548
            D   +R   QSL     ++ +    E + L  +   Q+AEI+ L+A ++ L +   +L+
Sbjct: 492 QDALYLREQAQSLRVSAKTLSNLQSYEQKGLEDKCAKQMAEIKSLKALIEKLLKEKLQLQ 551

Query: 549 LIIDMYRRESTESREVLEARDLEYKAWAHVQSLKSSLDERNLESRVKIANEAEAISQQRL 608
            +  +  RE  + R + E +D + KA A  + LK+ LDE  LE RVK A+E E+  Q+RL
Sbjct: 552 NLASICTRECNDDRGLAEIKDSQRKAQAQAEELKNVLDEHFLELRVKAAHETESACQERL 611

Query: 609 AAAEAEIARLRQKLEASKRDLTRLTDALKSKGDENVAYLSEIETIGQAYDDMQTQNQHLL 668
           A A+AEIA LR +L+ S+R++  L + +K K  E  A ++E+ETIGQAY+DMQTQNQHLL
Sbjct: 612 ATAKAEIAELRTQLDLSEREVLELKEGIKVKEQEAEASIAEMETIGQAYEDMQTQNQHLL 671

Query: 669 QQITERDDYNIKLVLEGVRARQMQEIMLIEKQTLEKEIQQANASLVLYEMKAARIEDQLR 728
           QQ+ ERDDYNIKLV E V+ +      L EKQ +EK++ Q NAS+  ++ + A  E+Q++
Sbjct: 672 QQVAERDDYNIKLVSESVKTKHAYNTHLSEKQVMEKQLHQVNASVENFKARIAHNEEQMK 731

Query: 729 GCSDHIQKIEDNKLQDTDTLENSQKRLLDIRIASQQTRESLDQSQSKVEKCRTAQAELQI 788
           GC     K+         +LE ++  + D     +  + ++  S+ + E+      ++++
Sbjct: 732 GCFSEAYKLIQEDRHLVISLETTKWEVADADKEFRWLKSAVSSSEKEYEQISRRTDDIKL 791

Query: 789 ELEKERFEKKRIEEELEVVGRKASRLEAQMESSSVVEKLHEELGEYEKIVNCKICVNSRK 836
           EL+ ER EKK++EEEL  + ++   L ++   +++V +L EE+   + I+ C +C +  K
Sbjct: 792 ELDDERREKKKLEEELMELNKELEELGSESVEAAIV-RLQEEVKNCKNILKCGVCFDRPK 842

BLAST of Lag0020596 vs. TAIR 10
Match: AT1G55250.1 (histone mono-ubiquitination 2 )

HSP 1 Score: 334.0 bits (855), Expect = 4.3e-91
Identity = 269/859 (31.32%), Postives = 452/859 (52.62%), Query Frame = 0

Query: 9   RKRRHFSTISPTAATAKKTPFLPISE-----------------DKKLDV--AVLQYQNQK 68
           +K     ++SP +     +P  PI++                 D ++DV   VLQ QNQK
Sbjct: 12  KKPHLLDSVSPNSMARNSSPSHPIAKSVSFFDCDFSLLCLRLVDYEIDVDATVLQLQNQK 71

Query: 69  LIQKLEVQKVEFKSLQNKYAQLKEKQEPYDMTVAVVKNCWEELVNGLETSSVRMRRWRSK 128
           L+Q+L++QK +   +++K  +L+  Q  YD  +  V   W +LV+ L    VR     + 
Sbjct: 72  LVQQLDLQKKQLYDVESKIQELQLNQTSYDDELISVNQLWNQLVDDLILLGVRA---GAN 131

Query: 129 QDGEHTMAAADES---SSSFEDAVLSRLAETGATQSSSAYSSNEHMEE----ETESPSEK 188
           Q+  + +   D+      + ++  L RL +  +  +S +      +EE       S  E 
Sbjct: 132 QEALNYLDIVDKKRVPPCAADETFLCRLLQVDSLDTSKSDEVVRKVEEALALRHSSTMEL 191

Query: 189 TKTIERNIEASIENLWYLKDGLHAALLNELPKDDSFRNRAFGDLVK-EVRNMRLRVKDFL 248
               E  I+        +   LHA    E   D + +  +  DL+K E +N+R  +    
Sbjct: 192 MGLFENTIDTQKTKAESISQSLHAVKSTE---DATIQLSSINDLMKEESKNLREMIDALH 251

Query: 249 FKQKGLAIELQNHRDLDAKTKAELKVLKAELESAVAELEESNSKLTKLRAEHDAAKRDGF 308
            + K  + ++Q +    +  ++ELK LK +LE   AELEE+  KL  L+ + DAA     
Sbjct: 252 VRHKEHSEQIQAYISSHSTDQSELKHLKGQLEEIKAELEENRRKLITLKMQKDAACEGHV 311

Query: 309 PVLNLSGKHSGGGKVRDKQKDLRDMESSLKELKDQAVGRLAELNSLHEGRLKMLQQLSDI 368
               ++       K  DK K LR+++ S+ E+K  A GRL+EL +  E  L + +Q  DI
Sbjct: 312 TSPAIANGSLSPEKPVDKTK-LRELKDSIDEIKIMAEGRLSELQASQEYNLSLSRQCQDI 371

Query: 369 QNTMKSVKSISSSQSYLLLRDRIEKLKLEVYEQQALFEKLQVEKDNIIWREKELNIKNNI 428
           +N +K  + I SS+ Y L+ DRI     E+   + L E +Q E+  ++ R+KELN++   
Sbjct: 372 ENELKDDQYIYSSRLYSLINDRIHHWNAELDRYKILTEAIQAERSFVMRRDKELNLRAES 431

Query: 429 LDMLRRSSTVSDTRIDDLEMLIQKQKDEKKSMENKLAEVLKEPGRKKIISEFRALVSSFP 488
           L+     +T   +RI+ LE  +Q    EK  +E +  E +++  R+ I SEF A+ S+  
Sbjct: 432 LEAANHKTTTVGSRIEVLEKKLQSCIIEKNGLELETEEAIQDSERQDIKSEFIAMASTLS 491

Query: 489 EAMGSMQSQLHKYKEAASDVHSVRADMQSLSSIIDRMEKECESLSSRSKDQIAEIQKLQA 548
           + M  M++QL ++K+ A D   +R   QSL   +     E + L  +   Q+AEI+ L+A
Sbjct: 492 KEMEMMEAQLKRWKDTAQDALYLREQAQSLRVSLSNKADEQKGLEDKCAKQMAEIKSLKA 551

Query: 549 TVQDLTEVNRELKLIIDMYRRESTESREVLEARDLEYKAWAHVQSLKSSLDERNLESRVK 608
            ++ L +   +L+ +  +  RE  + R + E +D + KA A  + LK+ LDE  LE RVK
Sbjct: 552 LIEKLLKEKLQLQNLASICTRECNDDRGLAEIKDSQRKAQAQAEELKNVLDEHFLELRVK 611

Query: 609 IANEAEAISQQRLAAAEAEIARLRQKLEASKRDLTRLTDALKSKGDENVAYLSEIETIGQ 668
            A+E E+  Q+RLA A+AEIA LR +L+ S+R++  L + +K K  E  A ++E+ETIGQ
Sbjct: 612 AAHETESACQERLATAKAEIAELRTQLDLSEREVLELKEGIKVKEQEAEASIAEMETIGQ 671

Query: 669 AYDDMQTQNQHLLQQITERDDYNIKLVLEGVRARQMQEIMLIEKQTLEKEIQQANASLVL 728
           AY+DMQTQNQHLLQQ+ ERDDYNIKLV E V+ +      L EKQ +EK++ Q NAS+  
Sbjct: 672 AYEDMQTQNQHLLQQVAERDDYNIKLVSESVKTKHAYNTHLSEKQVMEKQLHQVNASVEN 731

Query: 729 YEMKAARIEDQLRGCSDHIQKIEDNKLQDTDTLENSQKRLLDIRIASQQTRESLDQSQSK 788
           ++ + A  E+Q++GC     K+         +LE ++  + D     +  + ++  S+ +
Sbjct: 732 FKARIAHNEEQMKGCFSEAYKLIQEDRHLVISLETTKWEVADADKEFRWLKSAVSSSEKE 791

Query: 789 VEKCRTAQAELQIELEKERFEKKRIEEELEVVGRKASRLEAQMESSSVVEKLHEELGEYE 841
            E+      ++++EL+ ER EKK++EEEL  + ++   L ++   +++V +L EE+   +
Sbjct: 792 YEQISRRTDDIKLELDDERREKKKLEEELMELNKELEELGSESVEAAIV-RLQEEVKNCK 851

BLAST of Lag0020596 vs. TAIR 10
Match: AT1G55250.4 (histone mono-ubiquitination 2 )

HSP 1 Score: 192.6 bits (488), Expect = 1.5e-48
Identity = 123/335 (36.72%), Postives = 203/335 (60.60%), Query Frame = 0

Query: 501 ECESLSSRSKDQIAEIQKLQATVQDLTEVNRELKLIIDMYRRESTESREVLEARDLEYKA 560
           E + L  +   Q+AEI+ L+A ++ L +   +L+ +  +  RE  + R + E +D + KA
Sbjct: 7   EQKGLEDKCAKQMAEIKSLKALIEKLLKEKLQLQNLASICTRECNDDRGLAEIKDSQRKA 66

Query: 561 WAHVQSLKSSLDERNLESRVKIANEAEAISQQRLAAAEAEIARLRQKLEASKRDLTRLTD 620
            A  + LK+ LDE  LE RVK A+E E+  Q+RLA A+AEIA LR +L+ S+R++  L +
Sbjct: 67  QAQAEELKNVLDEHFLELRVKAAHETESACQERLATAKAEIAELRTQLDLSEREVLELKE 126

Query: 621 ALKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITERDDYNIKLVLEGVRARQMQEI 680
            +K K  E  A ++E+ETIGQAY+DMQTQNQHLLQQ+ ERDDYNIKLV E V+ +     
Sbjct: 127 GIKVKEQEAEASIAEMETIGQAYEDMQTQNQHLLQQVAERDDYNIKLVSESVKTKHAYNT 186

Query: 681 MLIEKQTLEKEIQQANASLVLYEMKAARIEDQLRGCSDHIQKIEDNKLQDTDTLENSQKR 740
            L EKQ +EK++ Q NAS+  ++ + A  E+Q++GC     K+         +LE ++  
Sbjct: 187 HLSEKQVMEKQLHQVNASVENFKARIAHNEEQMKGCFSEAYKLIQEDRHLVISLETTKWE 246

Query: 741 LLDIRIASQQTRESLDQSQSKVEKCRTAQAELQIELEKERFEKKRIEEELEVVGRKASRL 800
           + D     +  + ++  S+ + E+      ++++EL+ ER EKK++EEEL  + ++   L
Sbjct: 247 VADADKEFRWLKSAVSSSEKEYEQISRRTDDIKLELDDERREKKKLEEELMELNKELEEL 306

Query: 801 EAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQ 836
            ++   +++V +L EE+   + I+ C +C +  K+
Sbjct: 307 GSESVEAAIV-RLQEEVKNCKNILKCGVCFDRPKE 340

BLAST of Lag0020596 vs. TAIR 10
Match: AT1G55250.2 (histone mono-ubiquitination 2 )

HSP 1 Score: 192.2 bits (487), Expect = 2.0e-48
Identity = 124/340 (36.47%), Postives = 205/340 (60.29%), Query Frame = 0

Query: 501 ECESLSSRSKDQIAEIQKLQATVQDLTEVNRELKLIIDMYRRESTESREVLEARDLEYKA 560
           E + L  +   Q+AEI+ L+A ++ L +   +L+ +  +  RE  + R + E +D + KA
Sbjct: 7   EQKGLEDKCAKQMAEIKSLKALIEKLLKEKLQLQNLASICTRECNDDRGLAEIKDSQRKA 66

Query: 561 WAHVQSLKSSLDERNLESRVKIANEAEAISQQRLAAAEAEIARLRQKLEASKRDLTRLTD 620
            A  + LK+ LDE  LE RVK A+E E+  Q+RLA A+AEIA LR +L+ S+R++  L +
Sbjct: 67  QAQAEELKNVLDEHFLELRVKAAHETESACQERLATAKAEIAELRTQLDLSEREVLELKE 126

Query: 621 ALKSKGDENVAYLSEIETIGQAYDDMQTQNQHLLQQITERDDYNIKLVLEGVRARQMQEI 680
            +K K  E  A ++E+ETIGQAY+DMQTQNQHLLQQ+ ERDDYNIKLV E V+ +     
Sbjct: 127 GIKVKEQEAEASIAEMETIGQAYEDMQTQNQHLLQQVAERDDYNIKLVSESVKTKHAYNT 186

Query: 681 MLIEKQTLEKEIQQANASLVLYEMKAARIEDQLRGCSDHIQKIEDNKLQDTDTLENSQKR 740
            L EKQ +EK++ Q NAS+  ++ + A  E+Q++GC     K+         +LE ++  
Sbjct: 187 HLSEKQVMEKQLHQVNASVENFKARIAHNEEQMKGCFSEAYKLIQEDRHLVISLETTKWE 246

Query: 741 LLDIRIASQQTRESLDQSQSKVEKCRTAQAELQIELEKERFEKKRIEEELEVVGRKASRL 800
           + D     +  + ++  S+ + E+      ++++EL+ ER EKK++EEEL  + ++   L
Sbjct: 247 VADADKEFRWLKSAVSSSEKEYEQISRRTDDIKLELDDERREKKKLEEELMELNKELEEL 306

Query: 801 EAQMESSSVVEKLHEELGEYEKIVNCKICVNSRKQESLKK 841
            ++   +++V +L EE+   + I+ C +C +  K+  + K
Sbjct: 307 GSESVEAAIV-RLQEEVKNCKNILKCGVCFDRPKEVVIVK 345