Lag0020189 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0020189
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	endonuclease MutS2 isoform X1
Location	chr5: 48699993 .. 48701462 (+)
RNA-Seq Expression	Lag0020189
Synteny	Lag0020189

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTATTGTTGTAGTGCTGCAGGGATTGGTACCATCCTAGAGCCACTGTCTGCCGTTCCTTTAAACGATGAGTTGCAACGAGCGAGGGCATCAGTGGAAAAAGCTGAGGAAGATGTTCTCTTTATGCTAACTGAAAAAGTATGGGTGAGATGATTCAAAAATAATATATCATCATCAATAATGTCAAAAGTTAGTACATCTGAGTTAATTTTGTAAACTACGTTATTTTTTACAACCATTAAGTTTAAAGTTTAAACTCTTCATTCTGAAGTGTAGAGAAATTATAATGTTTATATTTACATTTTGAGTATCTCGGTTGATGATATGATGTTAAATCATATTTAATCCTAAAATTTTAAGCTTTTAGGTTAGAACTAATTTAGTTTTATATCAATTAATAATATGTGAGGCCTCCAATATTACACACTTATCAGTTATCAGAGAAAGGGTAAAAAGGGAATAGTCTGATTTTTGGTTATGGTGGGGACTGTTGGGATTGTGGTTCTGTGTACGGTTGTTGGGGGCAGAAGGGTTTTGTAGGGGTGTTTGGGCTATGGGTGAGTGTGTGTTTTTTGGTAGACTTGTGAGTCAAGGAGAGAATTCCAGCTCTCTCTAATTGCTGGGAACTTGTATTTGTTTCAGTGTTGGATCTTCTATTCCTCTGTTTGTGTATGTATTCTGTGTGTTTGACAGGTTATTTGTTTGGATAGTAACATAATGTTTTAAAAATCTTATTTATGTTTCATGACAGGTGAAAATGGAACTTGAAGACATTAATAAACTTATTGACTGTATAATTGAATTAGATGTGGTAAGTAATTATCACTACACCCTCAATTCCCTTCTCTCCCTGACTTAACTATTCTGGTGAATTATGTTTATCATTTTATCTATGAGCTCTCATATTCCCCAAGACTTCCCATTTCGTTCTTTGGCCAATTCGAAATGTTTTCAATTATATGTATCCTATTTGTGGAAAACTATTAATCGAGCGAAGGTGAGCAAGTATAGAGTGGTGCTCTCCCCTCTCTCCTCTCTCCCCTACCCTTTCCCCATCCTTAACTCAAGATTCCGGCCATGACCACCACCACCTCCACCACAACACCATGGAACGACTCCATTATATCAATCTATATTGATCGGAAATCCTTCACCATTGAATTTGATGAATCTTTTAGGGGAAGTCGAGCAAAAATTACAGAACATAGTAGGGCCTCTTCCCATTCCTTATCCCTCTCATGGAAGTCCCTCAACTGTCTTGCATCCTTCTTCAATACTCTTGCCCATCAACCGTGCTCGTTCAAATTTTCCTCTAAGATCAATATGTACTTTGGTTGGAAAAACTCAGCAATAAATATGGCTTCTTTGTGGAGATTAATCAGTTGCAGAATTTTGGTGTCGGCGTCGGCTCCTCATACCATACGAGGAAAACAAGGAAGGATGGTTCTTCTTCTTCTCCCTCATCTCTGA

mRNA sequence

ATGTATTGTTGTAGTGCTGCAGGGATTGGTACCATCCTAGAGCCACTGTCTGCCGTTCCTTTAAACGATGAGTTGCAACGAGCGAGGGCATCAGTGGAAAAAGCTGAGGAAGATGTTCTCTTTATGCTAACTGAAAAAGTGAAAATGGAACTTGAAGACATTAATAAACTTATTGACTGTATAATTGAATTAGATGTGATTCCGGCCATGACCACCACCACCTCCACCACAACACCATGGAACGACTCCATTATATCAATCTATATTGATCGGAAATCCTTCACCATTGAATTTGATGAATCTTTTAGGGGAAGTCGAGCAAAAATTACAGAACATAGTAGGGCCTCTTCCCATTCCTTATCCCTCTCATGGAAGTCCCTCAACTGTCTTGCATCCTTCTTCAATACTCTTGCCCATCAACCGTGCTCGTTCAAATTTTCCTCTAAGATCAATATGTACTTTGGTTGGAAAAACTCAGCAATAAATATGGCTTCTTTGTGGAGATTAATCAGTTGCAGAATTTTGGTGTCGGCGTCGGCTCCTCATACCATACGAGGAAAACAAGGAAGGATGGTTCTTCTTCTTCTCCCTCATCTCTGA

Coding sequence (CDS)

ATGTATTGTTGTAGTGCTGCAGGGATTGGTACCATCCTAGAGCCACTGTCTGCCGTTCCTTTAAACGATGAGTTGCAACGAGCGAGGGCATCAGTGGAAAAAGCTGAGGAAGATGTTCTCTTTATGCTAACTGAAAAAGTGAAAATGGAACTTGAAGACATTAATAAACTTATTGACTGTATAATTGAATTAGATGTGATTCCGGCCATGACCACCACCACCTCCACCACAACACCATGGAACGACTCCATTATATCAATCTATATTGATCGGAAATCCTTCACCATTGAATTTGATGAATCTTTTAGGGGAAGTCGAGCAAAAATTACAGAACATAGTAGGGCCTCTTCCCATTCCTTATCCCTCTCATGGAAGTCCCTCAACTGTCTTGCATCCTTCTTCAATACTCTTGCCCATCAACCGTGCTCGTTCAAATTTTCCTCTAAGATCAATATGTACTTTGGTTGGAAAAACTCAGCAATAAATATGGCTTCTTTGTGGAGATTAATCAGTTGCAGAATTTTGGTGTCGGCGTCGGCTCCTCATACCATACGAGGAAAACAAGGAAGGATGGTTCTTCTTCTTCTCCCTCATCTCTGA

Protein sequence

MYCCSAAGIGTILEPLSAVPLNDELQRARASVEKAEEDVLFMLTEKVKMELEDINKLIDCIIELDVIPAMTTTTSTTTPWNDSIISIYIDRKSFTIEFDESFRGSRAKITEHSRASSHSLSLSWKSLNCLASFFNTLAHQPCSFKFSSKINMYFGWKNSAINMASLWRLISCRILVSASAPHTIRGKQGRMVLLLLPHL

Homology

[Close]

BLAST of Lag0020189 vs. NCBI nr
Match: XP_022985051.1 (uncharacterized protein LOC111483140 isoform X2 [Cucurbita maxima])

HSP 1 Score: 108.6 bits (270), Expect = 6.2e-20
Identity = 57/65 (87.69%), Postives = 61/65 (93.85%), Query Frame = 0


Query: 5   SAAGIGTILEPLSAVPLNDELQRARASVEKAEEDVLFMLTEKVKMELEDINKLIDCIIEL 64
           SAAGIGTILEPLSAVPLNDELQ+ARA+V KAEEDVLFMLTEKVKM+ EDINKLI CIIEL
Sbjct: 281 SAAGIGTILEPLSAVPLNDELQQARAAVAKAEEDVLFMLTEKVKMDFEDINKLIGCIIEL 340

Query: 65  DVIPA 70
           DV+ A
Sbjct: 341 DVVNA 345

[Close]

BLAST of Lag0020189 vs. NCBI nr
Match: XP_022985053.1 (uncharacterized protein LOC111483140 isoform X4 [Cucurbita maxima])

HSP 1 Score: 108.6 bits (270), Expect = 6.2e-20
Identity = 57/65 (87.69%), Postives = 61/65 (93.85%), Query Frame = 0


Query: 5   SAAGIGTILEPLSAVPLNDELQRARASVEKAEEDVLFMLTEKVKMELEDINKLIDCIIEL 64
           SAAGIGTILEPLSAVPLNDELQ+ARA+V KAEEDVLFMLTEKVKM+ EDINKLI CIIEL
Sbjct: 281 SAAGIGTILEPLSAVPLNDELQQARAAVAKAEEDVLFMLTEKVKMDFEDINKLIGCIIEL 340

Query: 65  DVIPA 70
           DV+ A
Sbjct: 341 DVVNA 345

[Close]

BLAST of Lag0020189 vs. NCBI nr
Match: KAG7014662.1 (mutS2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 108.2 bits (269), Expect = 8.1e-20
Identity = 57/65 (87.69%), Postives = 61/65 (93.85%), Query Frame = 0


Query: 5   SAAGIGTILEPLSAVPLNDELQRARASVEKAEEDVLFMLTEKVKMELEDINKLIDCIIEL 64
           SAAGIGTILEPLSAVPLNDELQ+ARA+V KAEEDVLFMLTEKVKM+ EDINKLI CIIEL
Sbjct: 281 SAAGIGTILEPLSAVPLNDELQQARAAVAKAEEDVLFMLTEKVKMDFEDINKLIRCIIEL 340

Query: 65  DVIPA 70
           DV+ A
Sbjct: 341 DVVNA 345

[Close]

BLAST of Lag0020189 vs. NCBI nr
Match: KAG6576609.1 (hypothetical protein SDJN03_24183, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 108.2 bits (269), Expect = 8.1e-20
Identity = 57/65 (87.69%), Postives = 61/65 (93.85%), Query Frame = 0


Query: 5   SAAGIGTILEPLSAVPLNDELQRARASVEKAEEDVLFMLTEKVKMELEDINKLIDCIIEL 64
           SAAGIGTILEPLSAVPLNDELQ+ARA+V KAEEDVLFMLTEKVKM+ EDINKLI CIIEL
Sbjct: 281 SAAGIGTILEPLSAVPLNDELQQARAAVAKAEEDVLFMLTEKVKMDFEDINKLIRCIIEL 340

Query: 65  DVIPA 70
           DV+ A
Sbjct: 341 DVVNA 345

[Close]

BLAST of Lag0020189 vs. NCBI nr
Match: XP_023552491.1 (uncharacterized protein LOC111810138 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 108.2 bits (269), Expect = 8.1e-20
Identity = 57/65 (87.69%), Postives = 61/65 (93.85%), Query Frame = 0


Query: 5   SAAGIGTILEPLSAVPLNDELQRARASVEKAEEDVLFMLTEKVKMELEDINKLIDCIIEL 64
           SAAGIGTILEPLSAVPLNDELQ+ARA+V KAEEDVLFMLTEKVKM+ EDINKLI CIIEL
Sbjct: 281 SAAGIGTILEPLSAVPLNDELQQARAAVAKAEEDVLFMLTEKVKMDFEDINKLIRCIIEL 340

Query: 65  DVIPA 70
           DV+ A
Sbjct: 341 DVVNA 345

[Close]

BLAST of Lag0020189 vs. ExPASy TrEMBL
Match: A0A6J1JCF6 (uncharacterized protein LOC111483140 isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111483140 PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 3.0e-20
Identity = 57/65 (87.69%), Postives = 61/65 (93.85%), Query Frame = 0


Query: 5   SAAGIGTILEPLSAVPLNDELQRARASVEKAEEDVLFMLTEKVKMELEDINKLIDCIIEL 64
           SAAGIGTILEPLSAVPLNDELQ+ARA+V KAEEDVLFMLTEKVKM+ EDINKLI CIIEL
Sbjct: 281 SAAGIGTILEPLSAVPLNDELQQARAAVAKAEEDVLFMLTEKVKMDFEDINKLIGCIIEL 340

Query: 65  DVIPA 70
           DV+ A
Sbjct: 341 DVVNA 345

[Close]

BLAST of Lag0020189 vs. ExPASy TrEMBL
Match: A0A6J1J709 (uncharacterized protein LOC111483140 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483140 PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 3.0e-20
Identity = 57/65 (87.69%), Postives = 61/65 (93.85%), Query Frame = 0


Query: 5   SAAGIGTILEPLSAVPLNDELQRARASVEKAEEDVLFMLTEKVKMELEDINKLIDCIIEL 64
           SAAGIGTILEPLSAVPLNDELQ+ARA+V KAEEDVLFMLTEKVKM+ EDINKLI CIIEL
Sbjct: 281 SAAGIGTILEPLSAVPLNDELQQARAAVAKAEEDVLFMLTEKVKMDFEDINKLIGCIIEL 340

Query: 65  DVIPA 70
           DV+ A
Sbjct: 341 DVVNA 345

[Close]

BLAST of Lag0020189 vs. ExPASy TrEMBL
Match: A0A6J1E4M8 (uncharacterized protein LOC111430703 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111430703 PE=4 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 5.2e-20
Identity = 56/65 (86.15%), Postives = 61/65 (93.85%), Query Frame = 0


Query: 5   SAAGIGTILEPLSAVPLNDELQRARASVEKAEEDVLFMLTEKVKMELEDINKLIDCIIEL 64
           SAAGIGT+LEPLSAVPLNDELQ+ARA+V KAEEDVLFMLTEKVKM+ EDINKLI CIIEL
Sbjct: 281 SAAGIGTVLEPLSAVPLNDELQQARAAVAKAEEDVLFMLTEKVKMDFEDINKLIRCIIEL 340

Query: 65  DVIPA 70
           DV+ A
Sbjct: 341 DVVNA 345

[Close]

BLAST of Lag0020189 vs. ExPASy TrEMBL
Match: A0A6J1E586 (uncharacterized protein LOC111430703 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430703 PE=4 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 5.2e-20
Identity = 56/65 (86.15%), Postives = 61/65 (93.85%), Query Frame = 0


Query: 5   SAAGIGTILEPLSAVPLNDELQRARASVEKAEEDVLFMLTEKVKMELEDINKLIDCIIEL 64
           SAAGIGT+LEPLSAVPLNDELQ+ARA+V KAEEDVLFMLTEKVKM+ EDINKLI CIIEL
Sbjct: 281 SAAGIGTVLEPLSAVPLNDELQQARAAVAKAEEDVLFMLTEKVKMDFEDINKLIRCIIEL 340

Query: 65  DVIPA 70
           DV+ A
Sbjct: 341 DVVNA 345

[Close]

BLAST of Lag0020189 vs. ExPASy TrEMBL
Match: A0A6J1CK29 (uncharacterized protein LOC111011726 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111011726 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 4.4e-19
Identity = 54/65 (83.08%), Postives = 61/65 (93.85%), Query Frame = 0


Query: 5   SAAGIGTILEPLSAVPLNDELQRARASVEKAEEDVLFMLTEKVKMELEDINKLIDCIIEL 64
           +A GIGTILEPLSAVPLNDELQ+ARASV KAEEDVLFML+EKVKM+LEDI KLIDCII+L
Sbjct: 280 AAGGIGTILEPLSAVPLNDELQQARASVAKAEEDVLFMLSEKVKMDLEDIEKLIDCIIKL 339

Query: 65  DVIPA 70
           D++ A
Sbjct: 340 DMVNA 344

[Close]

BLAST of Lag0020189 vs. TAIR 10
Match: AT5G54090.1 (DNA mismatch repair protein MutS, type 2 )

HSP 1 Score: 60.8 bits (146), Expect = 1.4e-09
Identity = 35/68 (51.47%), Postives = 47/68 (69.12%), Query Frame = 0


Query: 5   SAAGIGTILEPLSAVPLNDELQRARASVEKAEEDVLFMLTEKVKMELEDINKLIDCIIEL 64
           S +G GT  EP++AV +ND+LQ ARASV KAE ++L MLTEK++  L  I  ++   I+L
Sbjct: 269 SGSGGGTAAEPIAAVSMNDDLQSARASVAKAEAEILSMLTEKMQDGLCQIEVVLSYSIQL 328

Query: 65  DVIPAMTT 73
           DVI A  T
Sbjct: 329 DVINARAT 336

The following BLAST results are available for this feature:

BLAST of Lag0020189 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022985051.1	6.2e-20	87.69	uncharacterized protein LOC111483140 isoform X2 [Cucurbita maxima]	[more]
XP_022985053.1	6.2e-20	87.69	uncharacterized protein LOC111483140 isoform X4 [Cucurbita maxima]	[more]
KAG7014662.1	8.1e-20	87.69	mutS2 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6576609.1	8.1e-20	87.69	hypothetical protein SDJN03_24183, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023552491.1	8.1e-20	87.69	uncharacterized protein LOC111810138 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0020189 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Lag0020189 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JCF6	3.0e-20	87.69	uncharacterized protein LOC111483140 isoform X4 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1J709	3.0e-20	87.69	uncharacterized protein LOC111483140 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1E4M8	5.2e-20	86.15	uncharacterized protein LOC111430703 isoform X4 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1E586	5.2e-20	86.15	uncharacterized protein LOC111430703 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1CK29	4.4e-19	83.08	uncharacterized protein LOC111011726 isoform X3 OS=Momordica charantia OX=3673 G...	[more]

BLAST of Lag0020189 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54090.1	1.4e-09	51.47	DNA mismatch repair protein MutS, type 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 18..45
None	No IPR available	PANTHER	PTHR11361:SF132	DNA MISMATCH REPAIR PROTEIN MUTS, TYPE 2	coord: 2..70
IPR045076	DNA mismatch repair MutS family	PANTHER	PTHR11361	DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER	coord: 2..70

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0020189.1	Lag0020189.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006298	mismatch repair
biological_process	GO:0045910	negative regulation of DNA recombination
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0030983	mismatched DNA binding