Lag0020147 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0020147
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptiondof zinc finger protein DOF5.7-like
Locationchr5: 48416033 .. 48417181 (+)
RNA-Seq ExpressionLag0020147
SyntenyLag0020147
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCTGCTCACTGGCCACAGGTATGGCTGATTGATTGACCCTTAACTTCTCTCTCTTTTTCTCATAAGTACATACATGAATGCAAATTCTTAGTCTTTCATTTATACAATCCTAAAAAATCACAACTTTAGAGTCGAAATTATCCGAGACTACAATAGAGTCGACAGTTTTGCACTTTTGGATGCGTGATGCAAAATTTAGGAGTTATTCAGAGGTGCACGAGTAGATTTTCGAATCCACATCCAAGCCTCCAAGGGTTATTAAGAGGGAGTTACATTATTATCGCAGGTTCGTCGGATAACTTTTTTGAATATTGGGTGATGCAGGTTGTGGTTAAAGGCATGGAAGAGGAAGAGGGACGAAGGGCAGTGATGGAAAGAAAGGCAAAGCCAGGGAAAGATCAAATTTTGAAGTGCCCCAGATGCAATTCCAACAACACAAAGTTCTGTTATTACAACAATTACAGCCTCTCACAGCCAAGGTACTTCTGCAAGTCTTGTAGAAGATATTGGACAGCAGGTGGATCTTTGAGGAACATTCCAGTGGGTGGGGCCTCAAGGAAGAACAAAAGACCTTCAGCTAATTTTTCATCATCAGCAGCAGCAGCTGCAGCTGCAGCTCCTTCCAGTTCATCACCAAACAACATGAACAATAACAAGAACAGTGATAATAATAATGGTGATGATCACAGCAACAGCAACCCCATTATCATCAACAATGGCAGCTCCCAACCAAATCTCAATACTTCTTCTTCTTCTTCTTCTTCTTCCAATACTGCTTGTTGTTGGCTCTCATCTGATCATATGAACCAAATGCTGAGATCAAGTAATGGGATGTCCCAAAGGGAGCTGATTCCCTTTATGCCTATGCCTGCAGCTCCACAGCCCACTGCCATGTGCACTGCTGCAGCTGATCATTGCAACCCTTCAAGGAATATGAACAGTAATAATAATAATGGGTTGTTTGAAGGGGAAGGTGGAGAAGCAAGGAGATTGTTCAGTAGTACTGTGGAGGATTTCAAGCAAATTCCAACCATGATTTCTGCTGATCAAAGTGGAGGAAAATTGGGGATGTTCCTGGTTTTTGGAATGGGATTTTTGGAGGAGGTTCATGGTGAGAAGATTGAGAAAATAAGCTTAAGGTGA

mRNA sequence

ATGGATTCTGCTCACTGGCCACAGGTTGTGGTTAAAGGCATGGAAGAGGAAGAGGGACGAAGGGCAGTGATGGAAAGAAAGGCAAAGCCAGGGAAAGATCAAATTTTGAAGTGCCCCAGATGCAATTCCAACAACACAAAGTTCTGTTATTACAACAATTACAGCCTCTCACAGCCAAGGTACTTCTGCAAGTCTTGTAGAAGATATTGGACAGCAGGTGGATCTTTGAGGAACATTCCAGTGGGTGGGGCCTCAAGGAAGAACAAAAGACCTTCAGCTAATTTTTCATCATCAGCAGCAGCAGCTGCAGCTGCAGCTCCTTCCAGTTCATCACCAAACAACATGAACAATAACAAGAACAGTGATAATAATAATGGTGATGATCACAGCAACAGCAACCCCATTATCATCAACAATGGCAGCTCCCAACCAAATCTCAATACTTCTTCTTCTTCTTCTTCTTCTTCCAATACTGCTTGTTGTTGGCTCTCATCTGATCATATGAACCAAATGCTGAGATCAAGTAATGGGATGTCCCAAAGGGAGCTGATTCCCTTTATGCCTATGCCTGCAGCTCCACAGCCCACTGCCATGTGCACTGCTGCAGCTGATCATTGCAACCCTTCAAGGAATATGAACAGTAATAATAATAATGGGTTGTTTGAAGGGGAAGGTGGAGAAGCAAGGAGATTGTTCAGTAGTACTGTGGAGGATTTCAAGCAAATTCCAACCATGATTTCTGCTGATCAAAGTGGAGGAAAATTGGGGATGTTCCTGGTTTTTGGAATGGGATTTTTGGAGGAGGTTCATGGTGAGAAGATTGAGAAAATAAGCTTAAGGTGA

Coding sequence (CDS)

ATGGATTCTGCTCACTGGCCACAGGTTGTGGTTAAAGGCATGGAAGAGGAAGAGGGACGAAGGGCAGTGATGGAAAGAAAGGCAAAGCCAGGGAAAGATCAAATTTTGAAGTGCCCCAGATGCAATTCCAACAACACAAAGTTCTGTTATTACAACAATTACAGCCTCTCACAGCCAAGGTACTTCTGCAAGTCTTGTAGAAGATATTGGACAGCAGGTGGATCTTTGAGGAACATTCCAGTGGGTGGGGCCTCAAGGAAGAACAAAAGACCTTCAGCTAATTTTTCATCATCAGCAGCAGCAGCTGCAGCTGCAGCTCCTTCCAGTTCATCACCAAACAACATGAACAATAACAAGAACAGTGATAATAATAATGGTGATGATCACAGCAACAGCAACCCCATTATCATCAACAATGGCAGCTCCCAACCAAATCTCAATACTTCTTCTTCTTCTTCTTCTTCTTCCAATACTGCTTGTTGTTGGCTCTCATCTGATCATATGAACCAAATGCTGAGATCAAGTAATGGGATGTCCCAAAGGGAGCTGATTCCCTTTATGCCTATGCCTGCAGCTCCACAGCCCACTGCCATGTGCACTGCTGCAGCTGATCATTGCAACCCTTCAAGGAATATGAACAGTAATAATAATAATGGGTTGTTTGAAGGGGAAGGTGGAGAAGCAAGGAGATTGTTCAGTAGTACTGTGGAGGATTTCAAGCAAATTCCAACCATGATTTCTGCTGATCAAAGTGGAGGAAAATTGGGGATGTTCCTGGTTTTTGGAATGGGATTTTTGGAGGAGGTTCATGGTGAGAAGATTGAGAAAATAAGCTTAAGGTGA

Protein sequence

MDSAHWPQVVVKGMEEEEGRRAVMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANFSSSAAAAAAAAPSSSSPNNMNNNKNSDNNNGDDHSNSNPIIINNGSSQPNLNTSSSSSSSSNTACCWLSSDHMNQMLRSSNGMSQRELIPFMPMPAAPQPTAMCTAAADHCNPSRNMNSNNNNGLFEGEGGEARRLFSSTVEDFKQIPTMISADQSGGKLGMFLVFGMGFLEEVHGEKIEKISLR
Homology
BLAST of Lag0020147 vs. NCBI nr
Match: XP_023553431.1 (dof zinc finger protein DOF5.7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 245.7 bits (626), Expect = 4.6e-61
Identity = 156/270 (57.78%), Postives = 171/270 (63.33%), Query Frame = 0

Query: 1   MDSAHWPQVVVKGME-EEEGRRAVMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQP 60
           MDSA WPQV VK ME EEEGR+A +ERKAKPGKDQIL CPRCNSNNTKFCYYNNYSLSQP
Sbjct: 1   MDSARWPQVEVKSMELEEEGRKAGLERKAKPGKDQILNCPRCNSNNTKFCYYNNYSLSQP 60

Query: 61  RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANFSSSAAAAAAAAPSSSSPNNMNNNK 120
           RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRP               PS + P+   NN 
Sbjct: 61  RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRP---------------PSPNFPSPPKNNN 120

Query: 121 NSDNNNGDDHSNSNPIIINNGSSQPNLNTSSSSSSSSNTACCWLSSDHMNQ--MLRSSNG 180
               +NGDD    N       S   N+NT     S++N A CWLSSD MN   M+RSS  
Sbjct: 121 KDYMSNGDDDDQGN-------SQSSNINT--PCPSATNIATCWLSSDDMNDQIMVRSSGI 180

Query: 181 MSQRELIPFMPM--PAAPQPTAMCTAAADHCNPSRNMNSNNNNGLFEGEGGEARRLFSST 240
           MS RELIPF+PM  PA PQPT   + A D CNPS                     LFS+T
Sbjct: 181 MSPRELIPFIPMPAPAPPQPTTSTSTAPDACNPSTT-------------------LFSTT 227

Query: 241 VEDFKQIPTMISAD-QSGGKLGMFLVFGMG 265
           VEDFKQ+P +IS D Q+G KLG    F  G
Sbjct: 241 VEDFKQLPPIISTDHQNGAKLGEVPAFWNG 227

BLAST of Lag0020147 vs. NCBI nr
Match: XP_022923076.1 (dof zinc finger protein DOF5.7-like [Cucurbita moschata])

HSP 1 Score: 245.0 bits (624), Expect = 7.8e-61
Identity = 156/270 (57.78%), Postives = 171/270 (63.33%), Query Frame = 0

Query: 1   MDSAHWPQVVVKGME-EEEGRRAVMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQP 60
           MDSA WPQV VK ME EEEGR+A +ERKAKPGKDQIL CPRCNSNNTKFCYYNNYSLSQP
Sbjct: 1   MDSAGWPQVEVKSMELEEEGRKAGLERKAKPGKDQILSCPRCNSNNTKFCYYNNYSLSQP 60

Query: 61  RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANFSSSAAAAAAAAPSSSSPNNMNNNK 120
           RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRP               PS + P+   NN 
Sbjct: 61  RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRP---------------PSPNFPSPPKNNN 120

Query: 121 NSDNNNGDDHSNSNPIIINNGSSQPNLNTSSSSSSSSNTACCWLSSDHMNQ--MLRSSNG 180
               +NGDD    N       S   N+NT     S++N A CWLSSD MN   M+RSS  
Sbjct: 121 KDYMSNGDDDDQGN-------SQTSNINT--PCPSATNIATCWLSSDDMNDQIMVRSSGI 180

Query: 181 MSQRELIPFMPM--PAAPQPTAMCTAAADHCNPSRNMNSNNNNGLFEGEGGEARRLFSST 240
           MS RELIPF+PM  PA PQPT     A D CNPS                     LF++T
Sbjct: 181 MSPRELIPFIPMPAPAPPQPTTSTITAPDACNPSTT-------------------LFTAT 227

Query: 241 VEDFKQIPTMISAD-QSGGKLGMFLVFGMG 265
           VEDFKQ+P +IS D Q+GGKLG    F  G
Sbjct: 241 VEDFKQLPPIISTDHQNGGKLGEVPAFWNG 227

BLAST of Lag0020147 vs. NCBI nr
Match: XP_022984510.1 (dof zinc finger protein DOF5.7-like [Cucurbita maxima])

HSP 1 Score: 245.0 bits (624), Expect = 7.8e-61
Identity = 156/270 (57.78%), Postives = 170/270 (62.96%), Query Frame = 0

Query: 1   MDSAHWPQVVVKGME-EEEGRRAVMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQP 60
           MDSA WPQV VK ME EEEGRRA +ERKAKPGKDQIL CPRCNSNNTKFCYYNNYSLSQP
Sbjct: 1   MDSARWPQVEVKSMELEEEGRRAGLERKAKPGKDQILNCPRCNSNNTKFCYYNNYSLSQP 60

Query: 61  RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANFSSSAAAAAAAAPSSSSPNNMNNNK 120
           RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRP               PS + P+   NN 
Sbjct: 61  RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRP---------------PSPNFPSPPKNNN 120

Query: 121 NSDNNNGDDHSNSNPIIINNGSSQPNLNTSSSSSSSSNTACCWLSSDHMNQ--MLRSSNG 180
               +NGDD    N       S   N+NT     S++N A CWLS D MN   M+RSS  
Sbjct: 121 KDYMSNGDDDDQGN-------SQSSNINT--PCPSATNIATCWLSCDGMNDQIMVRSSGI 180

Query: 181 MSQRELIPF--MPMPAAPQPTAMCTAAADHCNPSRNMNSNNNNGLFEGEGGEARRLFSST 240
           MS RELIPF  MP+PA PQPT     A D CNPS                     LF++T
Sbjct: 181 MSPRELIPFIPMPVPAPPQPTTSTITAPDACNPSTT-------------------LFTTT 227

Query: 241 VEDFKQIPTMISAD-QSGGKLGMFLVFGMG 265
           VEDFKQ+PT+IS D Q+G KLG    F  G
Sbjct: 241 VEDFKQLPTIISTDHQNGAKLGEVPAFWNG 227

BLAST of Lag0020147 vs. NCBI nr
Match: KAG6576640.1 (Dof zinc finger protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014692.1 Dof zinc finger protein DOF4.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 241.1 bits (614), Expect = 1.1e-59
Identity = 154/270 (57.04%), Postives = 169/270 (62.59%), Query Frame = 0

Query: 1   MDSAHWPQVVVKGME-EEEGRRAVMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQP 60
           MDSA WPQV  K ME EEEGR+A +ERKAKPGKDQIL CPRCNSNNTKFCYYNNYSLSQP
Sbjct: 1   MDSAGWPQVEAKSMELEEEGRKAGLERKAKPGKDQILNCPRCNSNNTKFCYYNNYSLSQP 60

Query: 61  RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANFSSSAAAAAAAAPSSSSPNNMNNNK 120
           RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRP               PS + P+   NN 
Sbjct: 61  RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRP---------------PSPNFPSPPKNNN 120

Query: 121 NSDNNNGDDHSNSNPIIINNGSSQPNLNTSSSSSSSSNTACCWLSSDHMNQ--MLRSSNG 180
               +NGDD    N       S   N+NT     S++N A CWLSSD MN   M+RSS  
Sbjct: 121 KDYMSNGDDDDQGN-------SQSSNINT--PCPSATNIATCWLSSDDMNDQIMVRSSGI 180

Query: 181 MSQRELIPFMPM--PAAPQPTAMCTAAADHCNPSRNMNSNNNNGLFEGEGGEARRLFSST 240
           MS RELIPF+PM  PA PQPT     A D CNPS                     LF++T
Sbjct: 181 MSPRELIPFIPMPAPAPPQPTTSTITAPDACNPSTT-------------------LFTAT 227

Query: 241 VEDFKQIPTMISAD-QSGGKLGMFLVFGMG 265
           V DFKQ+P +IS D Q+GGKLG    F  G
Sbjct: 241 VGDFKQLPPIISTDHQNGGKLGEVPAFWNG 227

BLAST of Lag0020147 vs. NCBI nr
Match: XP_004146271.1 (dof zinc finger protein DOF1.8 [Cucumis sativus] >XP_011651310.1 dof zinc finger protein DOF1.8 [Cucumis sativus] >XP_011651312.1 dof zinc finger protein DOF1.8 [Cucumis sativus] >XP_031737984.1 dof zinc finger protein DOF1.8 [Cucumis sativus] >XP_031737985.1 dof zinc finger protein DOF1.8 [Cucumis sativus] >KGN57649.1 hypothetical protein Csa_011544 [Cucumis sativus])

HSP 1 Score: 239.6 bits (610), Expect = 3.3e-59
Identity = 154/275 (56.00%), Postives = 174/275 (63.27%), Query Frame = 0

Query: 1   MDSAHWPQVVVKGME-EEEGRRAVMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQP 60
           MDSAHWPQV VK ME EEEG +AV+ERKAK  KDQIL CPRCNSNNTKFCYYNNYSLSQP
Sbjct: 1   MDSAHWPQVEVKNMELEEEGLKAVVERKAKARKDQILNCPRCNSNNTKFCYYNNYSLSQP 60

Query: 61  RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANFSSSAAAAAAAAPSSSSPNNMNNNK 120
           RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANF              S P + NN K
Sbjct: 61  RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANF--------------SLPPSKNNQK 120

Query: 121 NSDNNNGDDHSNSNPIIINNGSSQPNLNTSSSSSSSSN----TACCWLSS--DHMNQ--M 180
           N +N+ GDD         + G SQ N+N +S SS+++     T+CCWLSS  DHMN   M
Sbjct: 121 NYNNDGGDDD--------DQGISQLNINITSCSSTATTTNTATSCCWLSSDNDHMNNQIM 180

Query: 181 LRSSNGMSQRELIPFMPM--PAAPQPTAMCTAAADHCNPSRNMNSNNNNGLFEGEGGEAR 240
           LRS+  MSQRELIPF+PM  PA PQPTA+                               
Sbjct: 181 LRSNGIMSQRELIPFIPMPAPAPPQPTAV------------------------------- 218

Query: 241 RLFSSTVEDFKQIPTMISADQSGGKLGMFLVFGMG 265
               + +EDFKQ+ T+IS DQ+G KLG    F  G
Sbjct: 241 ----AALEDFKQLSTIISTDQNGAKLGDAPAFWSG 218

BLAST of Lag0020147 vs. ExPASy Swiss-Prot
Match: Q84JQ8 (Dof zinc finger protein DOF1.8 OS=Arabidopsis thaliana OX=3702 GN=DOF1.8 PE=2 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 3.0e-31
Identity = 79/131 (60.31%), Postives = 89/131 (67.94%), Query Frame = 0

Query: 1   MDSAHWPQ-VVVKGMEE------------EEGRRAVMERKAKPGKDQILKCPRCNSNNTK 60
           MD+A WPQ  VVK M E                   +ERKA+P KDQ L CPRCNS NTK
Sbjct: 1   MDTAKWPQEFVVKPMNEIVTNTCLKQQSNPPSPATPVERKARPEKDQALNCPRCNSLNTK 60

Query: 61  FCYYNNYSLSQPRYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANFSSSAAAAAAAAP 119
           FCYYNNYSL+QPRYFCK CRRYWTAGGSLRNIPVGG  RKNKR S+N SSS+ ++++   
Sbjct: 61  FCYYNNYSLTQPRYFCKDCRRYWTAGGSLRNIPVGGGVRKNKRSSSNSSSSSPSSSS--- 120

BLAST of Lag0020147 vs. ExPASy Swiss-Prot
Match: Q9SXG8 (Dof zinc finger protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DOF1 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 5.0e-26
Identity = 66/122 (54.10%), Postives = 89/122 (72.95%), Query Frame = 0

Query: 25  ERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGSLRNIPVGGA 84
           ER+A+P K++ L CPRCNS NTKFCYYNNYSL QPRYFCK+CRRYWT GGSLRN+PVGG 
Sbjct: 67  ERRARPQKEKALNCPRCNSTNTKFCYYNNYSLQQPRYFCKTCRRYWTEGGSLRNVPVGGG 126

Query: 85  SRKNKRPSANFSSSAAAAAAAAPSSSSPNNMNNNKNSDNNNGDDHSNSNPIIINNGSSQP 144
           SRKNKR S++ +S++ A+A+ A S  +  +M+ +  S        ++ NP +++ G+   
Sbjct: 127 SRKNKRSSSSAASASPASASTANSVVTSASMSMSMASTGGG----ASKNPKLVHEGAQDL 184

Query: 145 NL 147
           NL
Sbjct: 187 NL 184

BLAST of Lag0020147 vs. ExPASy Swiss-Prot
Match: O24463 (Dof zinc finger protein PBF OS=Zea mays OX=4577 GN=PBF PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 7.2e-25
Identity = 60/103 (58.25%), Postives = 76/103 (73.79%), Query Frame = 0

Query: 25  ERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGSLRNIPVGGA 84
           ERK +P   + LKCPRC+SNNTKFCYYNNYS+SQPRYFCK+CRRYWT GG+LRN+P+GG 
Sbjct: 49  ERKPRPQLPEALKCPRCDSNNTKFCYYNNYSMSQPRYFCKACRRYWTHGGTLRNVPIGGG 108

Query: 85  SRKNKRPS-------ANFSSSAAAAAAAAPSSSSPNNMNNNKN 121
            RKNK  S        + SSSA  A  +  +++S +NM+ NK+
Sbjct: 109 CRKNKHASRFVLGSHTSSSSSATYAPLSPSTNASSSNMSINKH 151

BLAST of Lag0020147 vs. ExPASy Swiss-Prot
Match: Q9FM03 (Dof zinc finger protein DOF5.6 OS=Arabidopsis thaliana OX=3702 GN=DOF5.6 PE=2 SV=2)

HSP 1 Score: 112.5 bits (280), Expect = 8.0e-24
Identity = 58/97 (59.79%), Postives = 70/97 (72.16%), Query Frame = 0

Query: 26  RKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGSLRNIPVGGAS 85
           R+ +P  D   KCPRC S +TKFCYYNNYSLSQPRYFCK+CRRYWT GG+LRNIPVGG  
Sbjct: 63  RRLRPPHDHPQKCPRCESTHTKFCYYNNYSLSQPRYFCKTCRRYWTKGGTLRNIPVGGGC 122

Query: 86  RKNKRPSANFSSSAAAAAAAAPSSSSPNNMNNNKNSD 123
           RKNK+PS++ SSS+ +      S   P+N+     SD
Sbjct: 123 RKNKKPSSSNSSSSTS------SGKKPSNIVTANTSD 153

BLAST of Lag0020147 vs. ExPASy Swiss-Prot
Match: B9F1L8 (Dof zinc finger protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=DOF2 PE=2 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 3.0e-23
Identity = 75/150 (50.00%), Postives = 94/150 (62.67%), Query Frame = 0

Query: 19  GRRAVMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGSLRN 78
           G  A  ER+A+P K++ L CPRCNS NTKFCYYNNYSL QPRYFCK+CRRYWT GGSLRN
Sbjct: 63  GAGAGTERRARPQKEKALNCPRCNSTNTKFCYYNNYSLQQPRYFCKTCRRYWTEGGSLRN 122

Query: 79  IPVGGASRKNKRPSANFSSSAAAAA--AAAPSSSSP-------NNMNNNKNSDNNNGDDH 138
           +PVGG SRKNKR S++   SAAA+A  +AA S S P         + +    D N    H
Sbjct: 123 VPVGGGSRKNKRSSSSVVPSAAASASTSAAVSGSVPVGLAAKNPKLMHEGAQDLNLAFPH 182

Query: 139 SNSNPIIINNGSSQPNLNTSSSSSSSSNTA 160
            +   +     ++ P+L +SS  +   N A
Sbjct: 183 HHGRALQPPEFTAFPSLESSSVCNPGGNLA 212

BLAST of Lag0020147 vs. ExPASy TrEMBL
Match: A0A6J1J8T6 (dof zinc finger protein DOF5.7-like OS=Cucurbita maxima OX=3661 GN=LOC111482784 PE=4 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 3.8e-61
Identity = 156/270 (57.78%), Postives = 170/270 (62.96%), Query Frame = 0

Query: 1   MDSAHWPQVVVKGME-EEEGRRAVMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQP 60
           MDSA WPQV VK ME EEEGRRA +ERKAKPGKDQIL CPRCNSNNTKFCYYNNYSLSQP
Sbjct: 1   MDSARWPQVEVKSMELEEEGRRAGLERKAKPGKDQILNCPRCNSNNTKFCYYNNYSLSQP 60

Query: 61  RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANFSSSAAAAAAAAPSSSSPNNMNNNK 120
           RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRP               PS + P+   NN 
Sbjct: 61  RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRP---------------PSPNFPSPPKNNN 120

Query: 121 NSDNNNGDDHSNSNPIIINNGSSQPNLNTSSSSSSSSNTACCWLSSDHMNQ--MLRSSNG 180
               +NGDD    N       S   N+NT     S++N A CWLS D MN   M+RSS  
Sbjct: 121 KDYMSNGDDDDQGN-------SQSSNINT--PCPSATNIATCWLSCDGMNDQIMVRSSGI 180

Query: 181 MSQRELIPF--MPMPAAPQPTAMCTAAADHCNPSRNMNSNNNNGLFEGEGGEARRLFSST 240
           MS RELIPF  MP+PA PQPT     A D CNPS                     LF++T
Sbjct: 181 MSPRELIPFIPMPVPAPPQPTTSTITAPDACNPSTT-------------------LFTTT 227

Query: 241 VEDFKQIPTMISAD-QSGGKLGMFLVFGMG 265
           VEDFKQ+PT+IS D Q+G KLG    F  G
Sbjct: 241 VEDFKQLPTIISTDHQNGAKLGEVPAFWNG 227

BLAST of Lag0020147 vs. ExPASy TrEMBL
Match: A0A6J1E5U7 (dof zinc finger protein DOF5.7-like OS=Cucurbita moschata OX=3662 GN=LOC111430855 PE=4 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 3.8e-61
Identity = 156/270 (57.78%), Postives = 171/270 (63.33%), Query Frame = 0

Query: 1   MDSAHWPQVVVKGME-EEEGRRAVMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQP 60
           MDSA WPQV VK ME EEEGR+A +ERKAKPGKDQIL CPRCNSNNTKFCYYNNYSLSQP
Sbjct: 1   MDSAGWPQVEVKSMELEEEGRKAGLERKAKPGKDQILSCPRCNSNNTKFCYYNNYSLSQP 60

Query: 61  RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANFSSSAAAAAAAAPSSSSPNNMNNNK 120
           RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRP               PS + P+   NN 
Sbjct: 61  RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRP---------------PSPNFPSPPKNNN 120

Query: 121 NSDNNNGDDHSNSNPIIINNGSSQPNLNTSSSSSSSSNTACCWLSSDHMNQ--MLRSSNG 180
               +NGDD    N       S   N+NT     S++N A CWLSSD MN   M+RSS  
Sbjct: 121 KDYMSNGDDDDQGN-------SQTSNINT--PCPSATNIATCWLSSDDMNDQIMVRSSGI 180

Query: 181 MSQRELIPFMPM--PAAPQPTAMCTAAADHCNPSRNMNSNNNNGLFEGEGGEARRLFSST 240
           MS RELIPF+PM  PA PQPT     A D CNPS                     LF++T
Sbjct: 181 MSPRELIPFIPMPAPAPPQPTTSTITAPDACNPSTT-------------------LFTAT 227

Query: 241 VEDFKQIPTMISAD-QSGGKLGMFLVFGMG 265
           VEDFKQ+P +IS D Q+GGKLG    F  G
Sbjct: 241 VEDFKQLPPIISTDHQNGGKLGEVPAFWNG 227

BLAST of Lag0020147 vs. ExPASy TrEMBL
Match: A0A0A0L9J3 (Dof-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G238150 PE=4 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 1.6e-59
Identity = 154/275 (56.00%), Postives = 174/275 (63.27%), Query Frame = 0

Query: 1   MDSAHWPQVVVKGME-EEEGRRAVMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQP 60
           MDSAHWPQV VK ME EEEG +AV+ERKAK  KDQIL CPRCNSNNTKFCYYNNYSLSQP
Sbjct: 1   MDSAHWPQVEVKNMELEEEGLKAVVERKAKARKDQILNCPRCNSNNTKFCYYNNYSLSQP 60

Query: 61  RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANFSSSAAAAAAAAPSSSSPNNMNNNK 120
           RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANF              S P + NN K
Sbjct: 61  RYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANF--------------SLPPSKNNQK 120

Query: 121 NSDNNNGDDHSNSNPIIINNGSSQPNLNTSSSSSSSSN----TACCWLSS--DHMNQ--M 180
           N +N+ GDD         + G SQ N+N +S SS+++     T+CCWLSS  DHMN   M
Sbjct: 121 NYNNDGGDDD--------DQGISQLNINITSCSSTATTTNTATSCCWLSSDNDHMNNQIM 180

Query: 181 LRSSNGMSQRELIPFMPM--PAAPQPTAMCTAAADHCNPSRNMNSNNNNGLFEGEGGEAR 240
           LRS+  MSQRELIPF+PM  PA PQPTA+                               
Sbjct: 181 LRSNGIMSQRELIPFIPMPAPAPPQPTAV------------------------------- 218

Query: 241 RLFSSTVEDFKQIPTMISADQSGGKLGMFLVFGMG 265
               + +EDFKQ+ T+IS DQ+G KLG    F  G
Sbjct: 241 ----AALEDFKQLSTIISTDQNGAKLGDAPAFWSG 218

BLAST of Lag0020147 vs. ExPASy TrEMBL
Match: A0A5D3CLI1 (Dof zinc finger protein DOF1.8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G001330 PE=4 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 3.6e-59
Identity = 153/268 (57.09%), Postives = 170/268 (63.43%), Query Frame = 0

Query: 1   MDSAHWPQVVVKGM--EEEEGRRAVMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQ 60
           MDSAHWPQV VK M  EEEEG +AV+ERKAK  KDQIL CPRCNSNNTKFCYYNNYSLSQ
Sbjct: 1   MDSAHWPQVEVKNMDLEEEEGIKAVVERKAKARKDQILNCPRCNSNNTKFCYYNNYSLSQ 60

Query: 61  PRYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANFSSSAAAAAAAAPSSSSPNNMNNN 120
           PRYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANF              S P + NN 
Sbjct: 61  PRYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANF--------------SLPPSKNNQ 120

Query: 121 KNSDNNNGDDHSNSNPIIINNGSSQPNLNTSSSSSSSSNT----ACCWLSS--DHMNQ-- 180
           KN +N  G D         + G SQ N+N +S SS+++ T     CCWLSS  DHMN   
Sbjct: 121 KNYNNGGGGDD--------DQGISQLNINITSCSSTATTTNTAATCCWLSSDNDHMNNQI 180

Query: 181 MLRSSNGMSQRELIPFMPM--PAAPQPTAMCTAAADHCNPSRNMNSNNNNGLFEGEGGEA 240
           MLRS+  MSQRELIPF+PM  PA PQPTA+  AA                          
Sbjct: 181 MLRSNGIMSQRELIPFIPMPAPAPPQPTAVSVAA-------------------------- 213

Query: 241 RRLFSSTVEDFKQIPTMISADQSGGKLG 257
                  +EDFKQ+ T+IS DQ+G KLG
Sbjct: 241 -------LEDFKQLSTIISTDQNGAKLG 213

BLAST of Lag0020147 vs. ExPASy TrEMBL
Match: A0A1S3C3F2 (dof zinc finger protein DOF1.8-like OS=Cucumis melo OX=3656 GN=LOC103496053 PE=4 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 3.6e-59
Identity = 153/268 (57.09%), Postives = 170/268 (63.43%), Query Frame = 0

Query: 1   MDSAHWPQVVVKGM--EEEEGRRAVMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQ 60
           MDSAHWPQV VK M  EEEEG +AV+ERKAK  KDQIL CPRCNSNNTKFCYYNNYSLSQ
Sbjct: 1   MDSAHWPQVEVKNMDLEEEEGIKAVVERKAKARKDQILNCPRCNSNNTKFCYYNNYSLSQ 60

Query: 61  PRYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANFSSSAAAAAAAAPSSSSPNNMNNN 120
           PRYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANF              S P + NN 
Sbjct: 61  PRYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANF--------------SLPPSKNNQ 120

Query: 121 KNSDNNNGDDHSNSNPIIINNGSSQPNLNTSSSSSSSSNT----ACCWLSS--DHMNQ-- 180
           KN +N  G D         + G SQ N+N +S SS+++ T     CCWLSS  DHMN   
Sbjct: 121 KNYNNGGGGDD--------DQGISQLNINITSCSSTATTTNTAATCCWLSSDNDHMNNQI 180

Query: 181 MLRSSNGMSQRELIPFMPM--PAAPQPTAMCTAAADHCNPSRNMNSNNNNGLFEGEGGEA 240
           MLRS+  MSQRELIPF+PM  PA PQPTA+  AA                          
Sbjct: 181 MLRSNGIMSQRELIPFIPMPAPAPPQPTAVSVAA-------------------------- 213

Query: 241 RRLFSSTVEDFKQIPTMISADQSGGKLG 257
                  +EDFKQ+ T+IS DQ+G KLG
Sbjct: 241 -------LEDFKQLSTIISTDQNGAKLG 213

BLAST of Lag0020147 vs. TAIR 10
Match: AT1G64620.1 (Dof-type zinc finger DNA-binding family protein )

HSP 1 Score: 137.1 bits (344), Expect = 2.1e-32
Identity = 79/131 (60.31%), Postives = 89/131 (67.94%), Query Frame = 0

Query: 1   MDSAHWPQ-VVVKGMEE------------EEGRRAVMERKAKPGKDQILKCPRCNSNNTK 60
           MD+A WPQ  VVK M E                   +ERKA+P KDQ L CPRCNS NTK
Sbjct: 1   MDTAKWPQEFVVKPMNEIVTNTCLKQQSNPPSPATPVERKARPEKDQALNCPRCNSLNTK 60

Query: 61  FCYYNNYSLSQPRYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANFSSSAAAAAAAAP 119
           FCYYNNYSL+QPRYFCK CRRYWTAGGSLRNIPVGG  RKNKR S+N SSS+ ++++   
Sbjct: 61  FCYYNNYSLTQPRYFCKDCRRYWTAGGSLRNIPVGGGVRKNKRSSSNSSSSSPSSSS--- 120

BLAST of Lag0020147 vs. TAIR 10
Match: AT5G62940.1 (Dof-type zinc finger DNA-binding family protein )

HSP 1 Score: 112.5 bits (280), Expect = 5.7e-25
Identity = 58/97 (59.79%), Postives = 70/97 (72.16%), Query Frame = 0

Query: 26  RKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGSLRNIPVGGAS 85
           R+ +P  D   KCPRC S +TKFCYYNNYSLSQPRYFCK+CRRYWT GG+LRNIPVGG  
Sbjct: 63  RRLRPPHDHPQKCPRCESTHTKFCYYNNYSLSQPRYFCKTCRRYWTKGGTLRNIPVGGGC 122

Query: 86  RKNKRPSANFSSSAAAAAAAAPSSSSPNNMNNNKNSD 123
           RKNK+PS++ SSS+ +      S   P+N+     SD
Sbjct: 123 RKNKKPSSSNSSSSTS------SGKKPSNIVTANTSD 153

BLAST of Lag0020147 vs. TAIR 10
Match: AT4G24060.1 (Dof-type zinc finger DNA-binding family protein )

HSP 1 Score: 108.2 bits (269), Expect = 1.1e-23
Identity = 99/280 (35.36%), Postives = 135/280 (48.21%), Query Frame = 0

Query: 1   MDSAHWPQ-VVVKGMEE----------------EEGRRAVMERKAKPGKDQILKCPRCNS 60
           MD+A WPQ +VVK +EE                ++      ERKA+P KDQ + CPRCNS
Sbjct: 1   MDTAQWPQEIVVKPLEEIVTNTCPKPQPQPLQPQQPPSVGGERKARPEKDQAVNCPRCNS 60

Query: 61  NNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGSLRNIPVGGASRKNKRP-------SANFS 120
            NTKFCYYNNYSL+QPRYFCK CRRYWT GGSLRNIPVGG SRKNKR        S N S
Sbjct: 61  TNTKFCYYNNYSLTQPRYFCKGCRRYWTEGGSLRNIPVGGGSRKNKRSHSSSSDISNNHS 120

Query: 121 SSAAAA---------------AAAAPSSSSPNNMNNNKNSDNNNGDDH----SNSNPIII 180
            S   A               +    +  +P  +   +   N     H    +N   +I 
Sbjct: 121 DSTQPATKKHLSDHHHHLMSMSQQGLTGQNPKFLETTQQDLNLGFSPHGMIRTNFTDLIH 180

Query: 181 NNGSSQPNLNTSSSSSSSSNTACCWLSSDHMNQMLRSSNGMSQRELIPFMPMPAAPQ-PT 228
           N G+   N N S+++++    + C   +     ++R+++         FM  P   Q P 
Sbjct: 181 NIGN---NTNKSNNNNNPLIVSSCSAMATSSLDLIRNNSNNGNSSNSSFMGFPVHNQDPA 240

BLAST of Lag0020147 vs. TAIR 10
Match: AT3G61850.1 (Dof-type zinc finger DNA-binding family protein )

HSP 1 Score: 103.2 bits (256), Expect = 3.4e-22
Identity = 72/135 (53.33%), Postives = 86/135 (63.70%), Query Frame = 0

Query: 22  AVME-RKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGSLRNIP 81
           A ME RKA+P   + + CPRCNS NTKFCYYNNYSL+QPRYFCK CRRYWT GGSLRN+P
Sbjct: 61  ATMETRKARP--QEKVNCPRCNSTNTKFCYYNNYSLTQPRYFCKGCRRYWTEGGSLRNVP 120

Query: 82  VGGASRKNKRPSANFSS-SAAAAAAAAP----SSSSPNNMNNNKN--SDNNNGDDHSNSN 141
           VGG+SRKNKR S   +S S        P    SS  PN  N + N  S     D H ++ 
Sbjct: 121 VGGSSRKNKRSSTPLASPSNPKLPDLNPPILFSSQIPNKSNKDLNLLSFPVMQDHHHHAL 180

Query: 142 PIIINNGSSQPNLNT 149
            ++ +NG S   +NT
Sbjct: 181 ELLRSNGVSSRGMNT 193

BLAST of Lag0020147 vs. TAIR 10
Match: AT3G61850.2 (Dof-type zinc finger DNA-binding family protein )

HSP 1 Score: 103.2 bits (256), Expect = 3.4e-22
Identity = 72/135 (53.33%), Postives = 86/135 (63.70%), Query Frame = 0

Query: 22  AVME-RKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGSLRNIP 81
           A ME RKA+P   + + CPRCNS NTKFCYYNNYSL+QPRYFCK CRRYWT GGSLRN+P
Sbjct: 49  ATMETRKARP--QEKVNCPRCNSTNTKFCYYNNYSLTQPRYFCKGCRRYWTEGGSLRNVP 108

Query: 82  VGGASRKNKRPSANFSS-SAAAAAAAAP----SSSSPNNMNNNKN--SDNNNGDDHSNSN 141
           VGG+SRKNKR S   +S S        P    SS  PN  N + N  S     D H ++ 
Sbjct: 109 VGGSSRKNKRSSTPLASPSNPKLPDLNPPILFSSQIPNKSNKDLNLLSFPVMQDHHHHAL 168

Query: 142 PIIINNGSSQPNLNT 149
            ++ +NG S   +NT
Sbjct: 169 ELLRSNGVSSRGMNT 181

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023553431.14.6e-6157.78dof zinc finger protein DOF5.7-like [Cucurbita pepo subsp. pepo][more]
XP_022923076.17.8e-6157.78dof zinc finger protein DOF5.7-like [Cucurbita moschata][more]
XP_022984510.17.8e-6157.78dof zinc finger protein DOF5.7-like [Cucurbita maxima][more]
KAG6576640.11.1e-5957.04Dof zinc finger protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG701... [more]
XP_004146271.13.3e-5956.00dof zinc finger protein DOF1.8 [Cucumis sativus] >XP_011651310.1 dof zinc finger... [more]
Match NameE-valueIdentityDescription
Q84JQ83.0e-3160.31Dof zinc finger protein DOF1.8 OS=Arabidopsis thaliana OX=3702 GN=DOF1.8 PE=2 SV... [more]
Q9SXG85.0e-2654.10Dof zinc finger protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DOF1 PE=2 ... [more]
O244637.2e-2558.25Dof zinc finger protein PBF OS=Zea mays OX=4577 GN=PBF PE=1 SV=1[more]
Q9FM038.0e-2459.79Dof zinc finger protein DOF5.6 OS=Arabidopsis thaliana OX=3702 GN=DOF5.6 PE=2 SV... [more]
B9F1L83.0e-2350.00Dof zinc finger protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=DOF2 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1J8T63.8e-6157.78dof zinc finger protein DOF5.7-like OS=Cucurbita maxima OX=3661 GN=LOC111482784 ... [more]
A0A6J1E5U73.8e-6157.78dof zinc finger protein DOF5.7-like OS=Cucurbita moschata OX=3662 GN=LOC11143085... [more]
A0A0A0L9J31.6e-5956.00Dof-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G238150 PE... [more]
A0A5D3CLI13.6e-5957.09Dof zinc finger protein DOF1.8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3C3F23.6e-5957.09dof zinc finger protein DOF1.8-like OS=Cucumis melo OX=3656 GN=LOC103496053 PE=4... [more]
Match NameE-valueIdentityDescription
AT1G64620.12.1e-3260.31Dof-type zinc finger DNA-binding family protein [more]
AT5G62940.15.7e-2559.79Dof-type zinc finger DNA-binding family protein [more]
AT4G24060.11.1e-2335.36Dof-type zinc finger DNA-binding family protein [more]
AT3G61850.13.4e-2253.33Dof-type zinc finger DNA-binding family protein [more]
AT3G61850.23.4e-2253.33Dof-type zinc finger DNA-binding family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003851Zinc finger, Dof-typePFAMPF02701zf-Dofcoord: 33..90
e-value: 2.0E-33
score: 114.3
IPR003851Zinc finger, Dof-typePROSITEPS01361ZF_DOF_1coord: 38..74
IPR003851Zinc finger, Dof-typePROSITEPS50884ZF_DOF_2coord: 36..90
score: 29.086916
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 89..152
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 77..152
NoneNo IPR availablePANTHERPTHR31992:SF124DOF ZINC FINGER PROTEIN DOF1.8coord: 21..216
NoneNo IPR availablePANTHERPTHR31992DOF ZINC FINGER PROTEIN DOF1.4-RELATEDcoord: 21..216

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0020147.1Lag0020147.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding