Lag0020144 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0020144
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	reticulocalbin-2
Location	chr5: 48400302 .. 48401844 (-)
RNA-Seq Expression	Lag0020144
Synteny	Lag0020144

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAGATTGGCGGAGAAACGTGAATTGGAAAGCGAAGGAGACGCCATTGGAGTGGGGAATCAAGTTCCCGATGCTTACAAACATTACTTCGACGAAGGAATATTGAACATCACGTTGAGGCTGATGGTCTTGTTTCCTTTGCTGGACCGTTCGCCGAAGGATGGAGCAATCAGTTACGATGAGTTGAACGATTGGATTACAGGGCAAGCCATGGAGAGATTGAATTACAGGACGCGGAAACAGTTGGCGTACTATGACAAGAATGGAGATGGCGCCATTTCATTCAACGAATATCTGCCACAGTTTACAGAGGAAGATATTGGTATGCCTTTCTTTTCTTTGATCATCGCTAGAAAGTTCATTACACAGAGGAAGAGAAATTTCATTGCATCTTGATTATACTTTTCATAATAGAGACTAAATCGTTATAAAATTTGAAGTATAAGGACTAGATGATTATAAATTATGAAAGCTGATAACCAAAAGTATTTTTTAACCTGATTGTTTTCTTGGTAACCAAAAGCATTATGGAATTGAGATTGAAAGTATATACTGAACATTCATGAAACAGCAATAAATGCAACGGGGCATGGAGAAGCTGGATGGTGGAAAAAGCAATTCACAAATGCGGATCTTGACCCAAATGGATTATTAAACTTTGATGAACTCAAAGAGTAATGTGCCATCTTCCTTTCCCTCCTGAAACAAAGCTCTCTACTTTACTATTTTTAACAACAATGAAAATACATGGAGAAAATGGTAAACGGTAATAAAGTACATAGTCTAAAATTCTCTAACGAGACTGGAATGAGATCTTTCTGTCCTGCAGTTTCTTGCACCCTGAAGATAGTAGCAATTACCGAATACAAAATTGGCTCTTGAGAGAGAAAATAAAGTAACCTTTCCCCATACTCTCTAGCTATAAACTTCTTTCATCTTCATGGTAATGGCAAGCCAAAGCATCAGCTTCTCTTTAACTTGCAGGCGCATGGACTCTGACAGAGATGGAAAACTCAACTTTGATGAATTTCTCCACCATACATATGACATATACAAGAGCTACATCGACTTCGAAACGCAAGGGGAGGACGTTCCGACCGCCGAAGAGAAGTTCGACGAGCTCGATCTCGACAAGGATGAGTATGCATTTGTTTTGATGGCAGTGAGTTATTTTTCTTCTTGATCCCAATTTGTTTGGTGTCTCTAAAATCAAGCAACCCTGTGCAGATTCTTGTCAGTGGAAGAATTAAGTCCATTGTTCCAGTATCTTCATCCTGGAGAACTGTCTTATGCCGAACATTACGCAAGCCATTTGATTAATGAGGTATCTCAGTAGTTGATTTGAAGAGATTAAGAAATCAAACAAAACATGATGAAGAAATCATCTGTTGATATGTTGTGATTAACATTGCAGGCTGATGATAACAAAGATGGCTACTTAACACTTGATGAGATGCTTAATCATGAGTTTGTTTTCTATACCACAGTATATGAAGATCGCAATGGCGACTATGAAGATGATTACCATGATGAACTTTAA

mRNA sequence

ATGGAAAGATTGGCGGAGAAACGTGAATTGGAAAGCGAAGGAGACGCCATTGGAGTGGGGAATCAAGTTCCCGATGCTTACAAACATTACTTCGACGAAGGAATATTGAACATCACGTTGAGGCTGATGGTCTTGTTTCCTTTGCTGGACCGTTCGCCGAAGGATGGAGCAATCAGTTACGATGAGTTGAACGATTGGATTACAGGGCAAGCCATGGAGAGATTGAATTACAGGACGCGGAAACAGTTGGCGTACTATGACAAGAATGGAGATGGCGCCATTTCATTCAACGAATATCTGCCACAGTTTACAGAGGAAGATATTGCAATAAATGCAACGGGGCATGGAGAAGCTGGATGGTGGAAAAAGCAATTCACAAATGCGGATCTTGACCCAAATGGATTATTAAACTTTGATGAACTCAAAGATTTCTTGCACCCTGAAGATAGTAGCAATTACCGAATACAAAATTGGCTCTTGAGAGAGAAAATAAAGCGCATGGACTCTGACAGAGATGGAAAACTCAACTTTGATGAATTTCTCCACCATACATATGACATATACAAGAGCTACATCGACTTCGAAACGCAAGGGGAGGACGTTCCGACCGCCGAAGAGAAGTTCGACGAGCTCGATCTCGACAAGGATGAATTCTTGTCAGTGGAAGAATTAAGTCCATTGTTCCAGTATCTTCATCCTGGAGAACTGTCTTATGCCGAACATTACGCAAGCCATTTGATTAATGAGGCTGATGATAACAAAGATGGCTACTTAACACTTGATGAGATGCTTAATCATGAGTTTGTTTTCTATACCACAGTATATGAAGATCGCAATGGCGACTATGAAGATGATTACCATGATGAACTTTAA

Coding sequence (CDS)

ATGGAAAGATTGGCGGAGAAACGTGAATTGGAAAGCGAAGGAGACGCCATTGGAGTGGGGAATCAAGTTCCCGATGCTTACAAACATTACTTCGACGAAGGAATATTGAACATCACGTTGAGGCTGATGGTCTTGTTTCCTTTGCTGGACCGTTCGCCGAAGGATGGAGCAATCAGTTACGATGAGTTGAACGATTGGATTACAGGGCAAGCCATGGAGAGATTGAATTACAGGACGCGGAAACAGTTGGCGTACTATGACAAGAATGGAGATGGCGCCATTTCATTCAACGAATATCTGCCACAGTTTACAGAGGAAGATATTGCAATAAATGCAACGGGGCATGGAGAAGCTGGATGGTGGAAAAAGCAATTCACAAATGCGGATCTTGACCCAAATGGATTATTAAACTTTGATGAACTCAAAGATTTCTTGCACCCTGAAGATAGTAGCAATTACCGAATACAAAATTGGCTCTTGAGAGAGAAAATAAAGCGCATGGACTCTGACAGAGATGGAAAACTCAACTTTGATGAATTTCTCCACCATACATATGACATATACAAGAGCTACATCGACTTCGAAACGCAAGGGGAGGACGTTCCGACCGCCGAAGAGAAGTTCGACGAGCTCGATCTCGACAAGGATGAATTCTTGTCAGTGGAAGAATTAAGTCCATTGTTCCAGTATCTTCATCCTGGAGAACTGTCTTATGCCGAACATTACGCAAGCCATTTGATTAATGAGGCTGATGATAACAAAGATGGCTACTTAACACTTGATGAGATGCTTAATCATGAGTTTGTTTTCTATACCACAGTATATGAAGATCGCAATGGCGACTATGAAGATGATTACCATGATGAACTTTAA

Protein sequence

MERLAEKRELESEGDAIGVGNQVPDAYKHYFDEGILNITLRLMVLFPLLDRSPKDGAISYDELNDWITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYLPQFTEEDIAINATGHGEAGWWKKQFTNADLDPNGLLNFDELKDFLHPEDSSNYRIQNWLLREKIKRMDSDRDGKLNFDEFLHHTYDIYKSYIDFETQGEDVPTAEEKFDELDLDKDEFLSVEELSPLFQYLHPGELSYAEHYASHLINEADDNKDGYLTLDEMLNHEFVFYTTVYEDRNGDYEDDYHDEL

Homology

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BLAST of Lag0020144 vs. NCBI nr
Match: XP_011651314.1 (reticulocalbin-2 [Cucumis sativus] >KGN57652.1 hypothetical protein Csa_010748 [Cucumis sativus])

HSP 1 Score: 489.6 bits (1259), Expect = 1.9e-134
Identity = 233/293 (79.52%), Postives = 264/293 (90.10%), Query Frame = 0


Query: 1   MERLAEKRELESEGDAIGVGNQ---VPDAYKHYFDEGILNITLRLMVLFPLLDRSPKDGA 60
           MERLA +   E   +AIGV NQ   + D+Y++Y+DEG LNI+LRL+VLFPLLD SPKDG 
Sbjct: 53  MERLAAEERGE---NAIGVDNQNHKIIDSYRNYYDEGRLNISLRLLVLFPLLDNSPKDGV 112

Query: 61  ISYDELNDWITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYLPQFTEEDIAINATGHGE 120
           ISY+EL+DWI GQA+ERLNYRT KQL +YDKNGD AISF+EYLPQFTEEDIA N TG+GE
Sbjct: 113 ISYEELSDWINGQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNETGYGE 172

Query: 121 AGWWKKQFTNADLDPNGLLNFDELKDFLHPEDSSNYRIQNWLLREKIKRMDSDRDGKLNF 180
           AGWW+KQFTNAD+D NGLL FDELKDFLHPEDSSNYRIQNWLL +K+KRMD D+DGKLNF
Sbjct: 173 AGWWRKQFTNADVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNF 232

Query: 181 DEFLHHTYDIYKSYIDFETQGEDVPTAEEKFDELDLDKDEFLSVEELSPLFQYLHPGELS 240
           DEFLHHTYDIYK+YI+FETQGEDVP+AEEKFDELDLD+DE LS EEL PLFQYLHPGE+S
Sbjct: 233 DEFLHHTYDIYKNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHPGEVS 292

Query: 241 YAEHYASHLINEADDNKDGYLTLDEMLNHEFVFYTTVYEDRNGDYEDDYHDEL 291
           YA+HY SHLINEADDNKDGYLT+DEMLNHE+VFY+TVYE++NGDYEDDYHDEL
Sbjct: 293 YAQHYTSHLINEADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDDYHDEL 342

[Close]

BLAST of Lag0020144 vs. NCBI nr
Match: XP_022141310.1 (reticulocalbin-2 [Momordica charantia])

HSP 1 Score: 488.8 bits (1257), Expect = 3.2e-134
Identity = 234/295 (79.32%), Postives = 257/295 (87.12%), Query Frame = 0


Query: 1   MERLAEKRELESEGDAIG-----VGNQVPDAYKHYFDEGILNITLRLMVLFPLLDRSPKD 60
           MER AE+       +AI        NQVPDAYK Y+DEG L I+LRL+VLFPLLD SPKD
Sbjct: 53  MERYAEEHGSGRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKD 112

Query: 61  GAISYDELNDWITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYLPQFTEEDIAINATGH 120
           G ISY+EL+DWI  QA++RLNYRTRK+L ++DKNGDGAISF EYLPQFTEEDIA   T H
Sbjct: 113 GVISYEELSDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAH 172

Query: 121 GEAGWWKKQFTNADLDPNGLLNFDELKDFLHPEDSSNYRIQNWLLREKIKRMDSDRDGKL 180
           GEAGWWK+QFTNAD DPNGLLNF+ELKDFLHPEDSSNYRIQNWLLREK+KRMD DRDGKL
Sbjct: 173 GEAGWWKRQFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKL 232

Query: 181 NFDEFLHHTYDIYKSYIDFETQGEDVPTAEEKFDELDLDKDEFLSVEELSPLFQYLHPGE 240
           NFDEFLHH +DIYKSYI+FETQG+DVPTAEEKFDELDLD+DE LSVEEL PLFQYLHPGE
Sbjct: 233 NFDEFLHHVFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGE 292

Query: 241 LSYAEHYASHLINEADDNKDGYLTLDEMLNHEFVFYTTVYEDRNGDYEDDYHDEL 291
           LSYA+HY SHLI EADDNKDGYL+LDEMLNHE+VFY +VYE RNGDYEDDYHDEL
Sbjct: 293 LSYAQHYTSHLIYEADDNKDGYLSLDEMLNHEYVFYNSVYEGRNGDYEDDYHDEL 347

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BLAST of Lag0020144 vs. NCBI nr
Match: XP_008455987.1 (PREDICTED: reticulocalbin-2 [Cucumis melo] >KAA0050524.1 reticulocalbin-2 [Cucumis melo var. makuwa])

HSP 1 Score: 483.4 bits (1243), Expect = 1.4e-132
Identity = 229/290 (78.97%), Postives = 262/290 (90.34%), Query Frame = 0


Query: 6   EKRELESEGD-AIGVGNQ---VPDAYKHYFDEGILNITLRLMVLFPLLDRSPKDGAISYD 65
           E+ E E  G+ AIGV NQ   + DAY++Y+D+G LNI+LRL+VLFPLLD SPKDG ISY+
Sbjct: 54  ERLEAEERGENAIGVENQNHKITDAYRNYYDDGRLNISLRLLVLFPLLDNSPKDGVISYE 113

Query: 66  ELNDWITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYLPQFTEEDIAINATGHGEAGWW 125
           EL+DWI GQA+ERLNYRT KQL +YDKNGD AISF+EYLPQFT+EDIA N TG+GEAGWW
Sbjct: 114 ELSDWINGQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNETGYGEAGWW 173

Query: 126 KKQFTNADLDPNGLLNFDELKDFLHPEDSSNYRIQNWLLREKIKRMDSDRDGKLNFDEFL 185
            KQFTNAD+D NGLL+FDELKDFLHPEDSSNYRIQNWLL +K+KRMD DRDGKLNFDEFL
Sbjct: 174 IKQFTNADVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFL 233

Query: 186 HHTYDIYKSYIDFETQGEDVPTAEEKFDELDLDKDEFLSVEELSPLFQYLHPGELSYAEH 245
           HHTYDIYK+YI+FETQG+D+PTAEEKFDELDLD+DE LSVEE+ PLFQYLHPGE+SYA+H
Sbjct: 234 HHTYDIYKNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQH 293

Query: 246 YASHLINEADDNKDGYLTLDEMLNHEFVFYTTVYEDRNGDYEDD-YHDEL 291
           Y SHLINEADDNKDGYLT+DEMLNHE+ FY+TVYE++NGDYEDD YHDEL
Sbjct: 294 YTSHLINEADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 343

[Close]

BLAST of Lag0020144 vs. NCBI nr
Match: TYK11288.1 (reticulocalbin-2 [Cucumis melo var. makuwa])

HSP 1 Score: 481.1 bits (1237), Expect = 6.7e-132
Identity = 228/290 (78.62%), Postives = 262/290 (90.34%), Query Frame = 0


Query: 6   EKRELESEGD-AIGVGNQ---VPDAYKHYFDEGILNITLRLMVLFPLLDRSPKDGAISYD 65
           E+ E E  G+ AIG+ NQ   + DAY++Y+DEG LNI+LRL+VLFPLLD SPKDG ISY+
Sbjct: 54  ERLEAEERGENAIGMENQNHKITDAYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYE 113

Query: 66  ELNDWITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYLPQFTEEDIAINATGHGEAGWW 125
           EL+DWI GQA+ERLNYRT KQL +YDKNGD AISF+EYLPQFT+EDIA N TG+GEAGWW
Sbjct: 114 ELSDWINGQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWW 173

Query: 126 KKQFTNADLDPNGLLNFDELKDFLHPEDSSNYRIQNWLLREKIKRMDSDRDGKLNFDEFL 185
            KQFTNAD+D NGLL+FDELKDFLHPEDSSNYRIQNWLL +K+KRMD DRDGKLNFDEFL
Sbjct: 174 IKQFTNADVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFL 233

Query: 186 HHTYDIYKSYIDFETQGEDVPTAEEKFDELDLDKDEFLSVEELSPLFQYLHPGELSYAEH 245
           HHTY+IYK+YI+FETQG+D+PTAEEKFDELDLD+DE LSVEE+ PLFQYLHPGE+SYA+H
Sbjct: 234 HHTYNIYKNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQH 293

Query: 246 YASHLINEADDNKDGYLTLDEMLNHEFVFYTTVYEDRNGDYEDD-YHDEL 291
           Y SHLINEADDNKDGYLT+DEMLNHE+ FY+TVYE++NGDYEDD YHDEL
Sbjct: 294 YTSHLINEADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 343

[Close]

BLAST of Lag0020144 vs. NCBI nr
Match: XP_038880351.1 (calumenin-B [Benincasa hispida])

HSP 1 Score: 475.3 bits (1222), Expect = 3.7e-130
Identity = 227/290 (78.28%), Postives = 254/290 (87.59%), Query Frame = 0


Query: 1   MERLAEKRELESEGDAIGVGNQVPDAYKHYFDEGILNITLRLMVLFPLLDRSPKDGAISY 60
           MERLAE+  +  E       NQV D  KHY+DEG LNI+LRL+VLFPLLD SPKDGAISY
Sbjct: 53  MERLAEENAIGVENQNSAKRNQVRDDCKHYYDEGSLNISLRLLVLFPLLDHSPKDGAISY 112

Query: 61  DELNDWITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYLPQFTEEDIAINATGHGEAGW 120
           +EL+DWITGQA+ERLNYRT KQLA+ DKNGDGAISF+EYLPQFTEEDIA N TG+GEAGW
Sbjct: 113 EELSDWITGQAIERLNYRTGKQLAFNDKNGDGAISFHEYLPQFTEEDIARNETGYGEAGW 172

Query: 121 WKKQFTNADLDPNGLLNFDELKDFLHPEDSSNYRIQNWLLREKIKRMDSDRDGKLNFDEF 180
           W+KQFTNAD+D N LLNFDELKDFLHPEDSSNYRIQNWLLR+K+ RMD D DGKL+FDEF
Sbjct: 173 WRKQFTNADVDQNNLLNFDELKDFLHPEDSSNYRIQNWLLRQKMNRMDHDSDGKLSFDEF 232

Query: 181 LHHTYDIYKSYIDFETQGEDVPTAEEKFDELDLDKDEFLSVEELSPLFQYLHPGELSYAE 240
           L   Y IYKSYI+FE QGED+P+AEEKFDELDLD+DE LSVEEL PLFQYLHPGE+SYA+
Sbjct: 233 LRQAYGIYKSYIEFEMQGEDIPSAEEKFDELDLDEDEVLSVEELRPLFQYLHPGEVSYAQ 292

Query: 241 HYASHLINEADDNKDGYLTLDEMLNHEFVFYTTVYEDRNGDYEDDYHDEL 291
           HY SHLIN+ADDNKDGYLTLDEMLNH+ VFY+TVYE+ NG+ EDDYHDEL
Sbjct: 293 HYTSHLINKADDNKDGYLTLDEMLNHDNVFYSTVYENNNGESEDDYHDEL 342

[Close]

BLAST of Lag0020144 vs. ExPASy Swiss-Prot
Match: Q8BP92 (Reticulocalbin-2 OS=Mus musculus OX=10090 GN=Rcn2 PE=1 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.3e-16
Identity = 75/263 (28.52%), Postives = 126/263 (47.91%), Query Frame = 0


Query: 41  RLMVLFPLLDRSPKDGAISYDELNDWITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYL 100
           RL  +   +D S  DG ++ +EL+ WI            ++Q   YDKN DGA++++EY 
Sbjct: 68  RLQSIIKKID-SDSDGFLTENELSQWIQMSFKHYAMQEAKQQFVEYDKNSDGAVTWDEYN 127

Query: 101 PQF------TEEDIAINATGHGEAGWW----KKQFTNADLDPNGLLNFDELKDFLHPEDS 160
            Q        +E+ A++ T  G         KK+F  A+ D    L+ +E   F HPE+ 
Sbjct: 128 IQMYDRVIDFDENTALDDTEEGSFRQLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEV 187

Query: 161 SNYRIQNWLLREKIKRMDSDRDGKLNFDEFLHHTYDIYKSYIDFETQGEDVPTAEEKF-D 220
               +  ++++E ++  D + DG ++ +EFL      Y+         E +   +++F +
Sbjct: 188 D--YMTEFVIQEALEEHDKNGDGFVSLEEFLGD----YRRDPTANEDPEWILVEKDRFVN 247

Query: 221 ELDLDKDEFLSVEELSPLFQYLHPGELSYAEHYASHLINEADDNKDGYLTLDEMLNHEFV 280
           + D D D  L  +E   L  ++ P     A+  A HLI+E D N D  L+ +E+L ++ +
Sbjct: 248 DYDKDNDGRLDPQE---LLSWVVPNNQGIAQEEALHLIDEMDLNSDKKLSEEEILENQDL 307

Query: 281 FYTTVYEDRNGDYEDD--YHDEL 291
           F T+   D      DD  YHDEL
Sbjct: 308 FLTSEATDYGRQLHDDYFYHDEL 320

[Close]

BLAST of Lag0020144 vs. ExPASy Swiss-Prot
Match: J3S9D9 (Reticulocalbin-2 OS=Crotalus adamanteus OX=8729 PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 6.3e-16
Identity = 74/263 (28.14%), Postives = 127/263 (48.29%), Query Frame = 0


Query: 41  RLMVLFPLLDRSPKDGAISYDELNDWITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYL 100
           RL V+   +D    DG ++  EL+ WI       +    ++Q  +YDK+GDG +S+ EY 
Sbjct: 54  RLKVIISRID-VDLDGFLTEAELSSWIQHSFKSYIIEDAKQQFQHYDKDGDGRVSWEEYN 113

Query: 101 PQF------TEEDIAINATGHGEAGWW----KKQFTNADLDPNGLLNFDELKDFLHPEDS 160
            Q        EED  ++              KK+F  A+ D +  L+F+E   F HPE++
Sbjct: 114 IQMYDRVIDFEEDTTLDDAEEESFRQLHLKDKKRFQKANKDGDSHLDFEEFAAFEHPEEA 173

Query: 161 SNYRIQNWLLREKIKRMDSDRDGKLNFDEFLHHTYDIYKSYIDFETQGEDVPTAEEKF-D 220
               ++ ++++E ++  D D DG ++  EFL      Y+     +   E +   E++F +
Sbjct: 174 D--YMKEFVIQESLEEHDKDGDGFVSLQEFLGD----YRRDPAAKEDPEWIVVEEDRFKN 233

Query: 221 ELDLDKDEFLSVEELSPLFQYLHPGELSYAEHYASHLINEADDNKDGYLTLDEMLNHEFV 280
           + D DKD  LS +E   L  ++ P     A+  A HL++E D + D  L+ +E+L ++ +
Sbjct: 234 DYDKDKDGKLSPKE---LLTWVMPNNEGLAQEEAVHLLDEMDLDGDRRLSANEILENQDL 293

Query: 281 FYTTVYEDRNGDYEDD--YHDEL 291
           F  +   D      D   YH+EL
Sbjct: 294 FLNSEATDYGRQLHDKSFYHEEL 306

[Close]

BLAST of Lag0020144 vs. ExPASy Swiss-Prot
Match: Q62703 (Reticulocalbin-2 OS=Rattus norvegicus OX=10116 GN=Rcn2 PE=1 SV=2)

HSP 1 Score: 86.3 bits (212), Expect = 6.3e-16
Identity = 74/263 (28.14%), Postives = 124/263 (47.15%), Query Frame = 0


Query: 41  RLMVLFPLLDRSPKDGAISYDELNDWITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYL 100
           RL  +   +D S  DG ++ +EL+ WI            ++Q   YDKN DG ++++EY 
Sbjct: 68  RLQSIIKKID-SDSDGFLTENELSQWIQMSFKHYAMQEAKQQFVEYDKNSDGTVTWDEYN 127

Query: 101 PQF------TEEDIAINATGHGEAGWW----KKQFTNADLDPNGLLNFDELKDFLHPEDS 160
            Q        +E+ A++ T            KK+F  A+ D    LN +E   F HPE+ 
Sbjct: 128 VQMYDRVIDFDENTALDDTEEESFRQLHLKDKKRFEKANQDSGPGLNLEEFIAFEHPEEV 187

Query: 161 SNYRIQNWLLREKIKRMDSDRDGKLNFDEFLHHTYDIYKSYIDFETQGEDVPTAEEKF-D 220
               +  ++++E ++  D + DG ++ +EFL      Y+         E +   +++F +
Sbjct: 188 D--YMTEFVIQEALEEHDKNGDGFVSLEEFLGD----YRRDPTANEDPEWILVEKDRFVN 247

Query: 221 ELDLDKDEFLSVEELSPLFQYLHPGELSYAEHYASHLINEADDNKDGYLTLDEMLNHEFV 280
           + D D D  L  +E   L  ++ P     A+  A HLI+E D N D  L+ +E+L ++ +
Sbjct: 248 DYDKDSDGRLDPQE---LLSWVVPNNQGIAQEEALHLIDEMDLNSDKKLSEEEILENQDL 307

Query: 281 FYTTVYEDRNGDYEDD--YHDEL 291
           F T+   D      DD  YHDEL
Sbjct: 308 FLTSEATDYGRQLHDDYFYHDEL 320

[Close]

BLAST of Lag0020144 vs. ExPASy Swiss-Prot
Match: B5X4E0 (Calumenin-B OS=Salmo salar OX=8030 GN=calub PE=2 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.7e-14
Identity = 66/236 (27.97%), Postives = 117/236 (49.58%), Query Frame = 0


Query: 41  RLMVLFPLLDRSPKDGAISYDELNDWITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYL 100
           RL +L   +D   KDG +S +E+  WI       +     +Q   +D NGDG +S+ EY 
Sbjct: 73  RLGMLVERIDED-KDGYVSVEEMKKWIKHSQKRWIYDDVDRQWKGHDHNGDGLVSWEEYK 132

Query: 101 PQ-----FTEEDIAINATGHGEAGWWKKQFTNADLDPNGLLNFDELKDFLHPEDSSNYRI 160
                    + D     +        +++F  +DLD +   N +E   FLHPE+  +  +
Sbjct: 133 NATYGYILDDPDPEDGFSYRQMISRDERRFKMSDLDADLKANKEEFTAFLHPEEYDH--M 192

Query: 161 QNWLLREKIKRMDSDRDGKLNFDEFLHHTYDIYKSYIDFETQGEDVPTAEEKFDEL-DLD 220
           ++ ++ E ++ +D + DG ++ +E++    D+Y    D  ++ E V T  E+F E  D +
Sbjct: 193 KDIVVLETMEDIDKNGDGFIDLEEYIG---DMYNQEGD-PSEPEWVRTEREQFTEFRDTN 252

Query: 221 KDEFLSVEELSPLFQYLHPGELSYAEHYASHLINEADDNKDGYLTLDEMLNHEFVF 271
           KD  +  EE      ++ P +  +AE  A HL+ E+D++KDG LT  E++    +F
Sbjct: 253 KDGRMDKEETK---DWILPSDYDHAEAEAKHLVYESDNDKDGKLTKAEIVEKYDLF 298

[Close]

BLAST of Lag0020144 vs. ExPASy Swiss-Prot
Match: Q14257 (Reticulocalbin-2 OS=Homo sapiens OX=9606 GN=RCN2 PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.5e-14
Identity = 80/300 (26.67%), Postives = 132/300 (44.00%), Query Frame = 0


Query: 4   LAEKRELESEGDAIGVGNQVPDAYKHYFDEGILNITLRLMVLFPLLDRSPKDGAISYDEL 63
           L E+R        +GV   V +  K   +E       RL  +   +D    DG ++  EL
Sbjct: 32  LGERRSDYDREALLGVQEDVDEYVKLGHEEQ----QKRLQAIIKKIDLD-SDGFLTESEL 91

Query: 64  NDWITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYLPQFTEEDIAIN---ATGHGEAGW 123
           + WI            ++Q   YDKN D  ++++EY  Q  +  I  +   A    E   
Sbjct: 92  SSWIQMSFKHYAMQEAKQQFVEYDKNSDDTVTWDEYNIQMYDRVIDFDENTALDDAEEES 151

Query: 124 W-------KKQFTNADLDPNGLLNFDELKDFLHPEDSSNYRIQNWLLREKIKRMDSDRDG 183
           +       KK+F  A+ D    L+ +E   F HPE+     +  ++++E ++  D + DG
Sbjct: 152 FRKLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVD--YMTEFVIQEALEEHDKNGDG 211

Query: 184 KLNFDEFLHHTYDIYKSYIDFETQGEDVPTAEEKF-DELDLDKDEFLSVEELSPLFQYLH 243
            ++ +EFL      Y+         E +   +++F ++ D D D  L  +EL P   ++ 
Sbjct: 212 FVSLEEFLGD----YRWDPTANEDPEWILVEKDRFVNDYDKDNDGRLDPQELLP---WVV 271

Query: 244 PGELSYAEHYASHLINEADDNKDGYLTLDEMLNHEFVFYTTVYEDRNGDYEDD--YHDEL 291
           P     A+  A HLI+E D N D  L+ +E+L +  +F T+   D      DD  YHDEL
Sbjct: 272 PNNQGIAQEEALHLIDEMDLNGDKKLSEEEILENPDLFLTSEATDYGRQLHDDYFYHDEL 317

[Close]

BLAST of Lag0020144 vs. ExPASy TrEMBL
Match: A0A0A0LAH3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G238180 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 9.2e-135
Identity = 233/293 (79.52%), Postives = 264/293 (90.10%), Query Frame = 0


Query: 1   MERLAEKRELESEGDAIGVGNQ---VPDAYKHYFDEGILNITLRLMVLFPLLDRSPKDGA 60
           MERLA +   E   +AIGV NQ   + D+Y++Y+DEG LNI+LRL+VLFPLLD SPKDG 
Sbjct: 53  MERLAAEERGE---NAIGVDNQNHKIIDSYRNYYDEGRLNISLRLLVLFPLLDNSPKDGV 112

Query: 61  ISYDELNDWITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYLPQFTEEDIAINATGHGE 120
           ISY+EL+DWI GQA+ERLNYRT KQL +YDKNGD AISF+EYLPQFTEEDIA N TG+GE
Sbjct: 113 ISYEELSDWINGQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNETGYGE 172

Query: 121 AGWWKKQFTNADLDPNGLLNFDELKDFLHPEDSSNYRIQNWLLREKIKRMDSDRDGKLNF 180
           AGWW+KQFTNAD+D NGLL FDELKDFLHPEDSSNYRIQNWLL +K+KRMD D+DGKLNF
Sbjct: 173 AGWWRKQFTNADVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNF 232

Query: 181 DEFLHHTYDIYKSYIDFETQGEDVPTAEEKFDELDLDKDEFLSVEELSPLFQYLHPGELS 240
           DEFLHHTYDIYK+YI+FETQGEDVP+AEEKFDELDLD+DE LS EEL PLFQYLHPGE+S
Sbjct: 233 DEFLHHTYDIYKNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHPGEVS 292

Query: 241 YAEHYASHLINEADDNKDGYLTLDEMLNHEFVFYTTVYEDRNGDYEDDYHDEL 291
           YA+HY SHLINEADDNKDGYLT+DEMLNHE+VFY+TVYE++NGDYEDDYHDEL
Sbjct: 293 YAQHYTSHLINEADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDDYHDEL 342

[Close]

BLAST of Lag0020144 vs. ExPASy TrEMBL
Match: A0A6J1CHQ2 (reticulocalbin-2 OS=Momordica charantia OX=3673 GN=LOC111011736 PE=4 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 1.6e-134
Identity = 234/295 (79.32%), Postives = 257/295 (87.12%), Query Frame = 0


Query: 1   MERLAEKRELESEGDAIG-----VGNQVPDAYKHYFDEGILNITLRLMVLFPLLDRSPKD 60
           MER AE+       +AI        NQVPDAYK Y+DEG L I+LRL+VLFPLLD SPKD
Sbjct: 53  MERYAEEHGSGRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKD 112

Query: 61  GAISYDELNDWITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYLPQFTEEDIAINATGH 120
           G ISY+EL+DWI  QA++RLNYRTRK+L ++DKNGDGAISF EYLPQFTEEDIA   T H
Sbjct: 113 GVISYEELSDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAH 172

Query: 121 GEAGWWKKQFTNADLDPNGLLNFDELKDFLHPEDSSNYRIQNWLLREKIKRMDSDRDGKL 180
           GEAGWWK+QFTNAD DPNGLLNF+ELKDFLHPEDSSNYRIQNWLLREK+KRMD DRDGKL
Sbjct: 173 GEAGWWKRQFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKL 232

Query: 181 NFDEFLHHTYDIYKSYIDFETQGEDVPTAEEKFDELDLDKDEFLSVEELSPLFQYLHPGE 240
           NFDEFLHH +DIYKSYI+FETQG+DVPTAEEKFDELDLD+DE LSVEEL PLFQYLHPGE
Sbjct: 233 NFDEFLHHVFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGE 292

Query: 241 LSYAEHYASHLINEADDNKDGYLTLDEMLNHEFVFYTTVYEDRNGDYEDDYHDEL 291
           LSYA+HY SHLI EADDNKDGYL+LDEMLNHE+VFY +VYE RNGDYEDDYHDEL
Sbjct: 293 LSYAQHYTSHLIYEADDNKDGYLSLDEMLNHEYVFYNSVYEGRNGDYEDDYHDEL 347

[Close]

BLAST of Lag0020144 vs. ExPASy TrEMBL
Match: A0A5A7U5L5 (Reticulocalbin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G001680 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 6.6e-133
Identity = 229/290 (78.97%), Postives = 262/290 (90.34%), Query Frame = 0


Query: 6   EKRELESEGD-AIGVGNQ---VPDAYKHYFDEGILNITLRLMVLFPLLDRSPKDGAISYD 65
           E+ E E  G+ AIGV NQ   + DAY++Y+D+G LNI+LRL+VLFPLLD SPKDG ISY+
Sbjct: 54  ERLEAEERGENAIGVENQNHKITDAYRNYYDDGRLNISLRLLVLFPLLDNSPKDGVISYE 113

Query: 66  ELNDWITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYLPQFTEEDIAINATGHGEAGWW 125
           EL+DWI GQA+ERLNYRT KQL +YDKNGD AISF+EYLPQFT+EDIA N TG+GEAGWW
Sbjct: 114 ELSDWINGQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNETGYGEAGWW 173

Query: 126 KKQFTNADLDPNGLLNFDELKDFLHPEDSSNYRIQNWLLREKIKRMDSDRDGKLNFDEFL 185
            KQFTNAD+D NGLL+FDELKDFLHPEDSSNYRIQNWLL +K+KRMD DRDGKLNFDEFL
Sbjct: 174 IKQFTNADVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFL 233

Query: 186 HHTYDIYKSYIDFETQGEDVPTAEEKFDELDLDKDEFLSVEELSPLFQYLHPGELSYAEH 245
           HHTYDIYK+YI+FETQG+D+PTAEEKFDELDLD+DE LSVEE+ PLFQYLHPGE+SYA+H
Sbjct: 234 HHTYDIYKNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQH 293

Query: 246 YASHLINEADDNKDGYLTLDEMLNHEFVFYTTVYEDRNGDYEDD-YHDEL 291
           Y SHLINEADDNKDGYLT+DEMLNHE+ FY+TVYE++NGDYEDD YHDEL
Sbjct: 294 YTSHLINEADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 343

[Close]

BLAST of Lag0020144 vs. ExPASy TrEMBL
Match: A0A1S3C255 (reticulocalbin-2 OS=Cucumis melo OX=3656 GN=LOC103496051 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 6.6e-133
Identity = 229/290 (78.97%), Postives = 262/290 (90.34%), Query Frame = 0


Query: 6   EKRELESEGD-AIGVGNQ---VPDAYKHYFDEGILNITLRLMVLFPLLDRSPKDGAISYD 65
           E+ E E  G+ AIGV NQ   + DAY++Y+D+G LNI+LRL+VLFPLLD SPKDG ISY+
Sbjct: 54  ERLEAEERGENAIGVENQNHKITDAYRNYYDDGRLNISLRLLVLFPLLDNSPKDGVISYE 113

Query: 66  ELNDWITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYLPQFTEEDIAINATGHGEAGWW 125
           EL+DWI GQA+ERLNYRT KQL +YDKNGD AISF+EYLPQFT+EDIA N TG+GEAGWW
Sbjct: 114 ELSDWINGQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNETGYGEAGWW 173

Query: 126 KKQFTNADLDPNGLLNFDELKDFLHPEDSSNYRIQNWLLREKIKRMDSDRDGKLNFDEFL 185
            KQFTNAD+D NGLL+FDELKDFLHPEDSSNYRIQNWLL +K+KRMD DRDGKLNFDEFL
Sbjct: 174 IKQFTNADVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFL 233

Query: 186 HHTYDIYKSYIDFETQGEDVPTAEEKFDELDLDKDEFLSVEELSPLFQYLHPGELSYAEH 245
           HHTYDIYK+YI+FETQG+D+PTAEEKFDELDLD+DE LSVEE+ PLFQYLHPGE+SYA+H
Sbjct: 234 HHTYDIYKNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQH 293

Query: 246 YASHLINEADDNKDGYLTLDEMLNHEFVFYTTVYEDRNGDYEDD-YHDEL 291
           Y SHLINEADDNKDGYLT+DEMLNHE+ FY+TVYE++NGDYEDD YHDEL
Sbjct: 294 YTSHLINEADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 343

[Close]

BLAST of Lag0020144 vs. ExPASy TrEMBL
Match: A0A5D3CJD2 (Reticulocalbin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G001480 PE=4 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 3.3e-132
Identity = 228/290 (78.62%), Postives = 262/290 (90.34%), Query Frame = 0


Query: 6   EKRELESEGD-AIGVGNQ---VPDAYKHYFDEGILNITLRLMVLFPLLDRSPKDGAISYD 65
           E+ E E  G+ AIG+ NQ   + DAY++Y+DEG LNI+LRL+VLFPLLD SPKDG ISY+
Sbjct: 54  ERLEAEERGENAIGMENQNHKITDAYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYE 113

Query: 66  ELNDWITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYLPQFTEEDIAINATGHGEAGWW 125
           EL+DWI GQA+ERLNYRT KQL +YDKNGD AISF+EYLPQFT+EDIA N TG+GEAGWW
Sbjct: 114 ELSDWINGQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWW 173

Query: 126 KKQFTNADLDPNGLLNFDELKDFLHPEDSSNYRIQNWLLREKIKRMDSDRDGKLNFDEFL 185
            KQFTNAD+D NGLL+FDELKDFLHPEDSSNYRIQNWLL +K+KRMD DRDGKLNFDEFL
Sbjct: 174 IKQFTNADVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFL 233

Query: 186 HHTYDIYKSYIDFETQGEDVPTAEEKFDELDLDKDEFLSVEELSPLFQYLHPGELSYAEH 245
           HHTY+IYK+YI+FETQG+D+PTAEEKFDELDLD+DE LSVEE+ PLFQYLHPGE+SYA+H
Sbjct: 234 HHTYNIYKNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQH 293

Query: 246 YASHLINEADDNKDGYLTLDEMLNHEFVFYTTVYEDRNGDYEDD-YHDEL 291
           Y SHLINEADDNKDGYLT+DEMLNHE+ FY+TVYE++NGDYEDD YHDEL
Sbjct: 294 YTSHLINEADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 343

[Close]

BLAST of Lag0020144 vs. TAIR 10
Match: AT4G27790.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 274.2 bits (700), Expect = 1.2e-73
Identity = 137/292 (46.92%), Postives = 195/292 (66.78%), Query Frame = 0


Query: 1   MERLAEKRELESEGDAIGVGNQVPDAYKHYF-DEGILNITLRLMVLFPLLDRSPKDGAIS 60
           +ERL+ ++E  ++        +  D ++ YF  E  LN T+R+  LFPLLD SP+DG +S
Sbjct: 55  IERLSHEKEAGTK-TVEAAKEEKDDMFEEYFAQERRLNTTMRIKFLFPLLDASPRDGFVS 114

Query: 61  YDELNDWITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYLPQFTEEDIAINATGHGEAG 120
             EL  W+  Q  + + YRT K+L   DK+ DG I+F EYLPQF+++DI  N  GHGEAG
Sbjct: 115 LKELQTWMMQQTEDNMVYRTAKELELQDKDKDGVITFEEYLPQFSKQDIEKNEKGHGEAG 174

Query: 121 WWKKQFTNADLDPNGLLNFDELKDFLHPEDSSNYRIQNWLLREKIKRMDSDRDGKLNFDE 180
           WW +QF N+D D NG L+ +E  +FLHPEDS N   Q W+L+E++  MD++ DGKL + E
Sbjct: 175 WWMEQFKNSDFDHNGSLDIEEFNNFLHPEDSRNGDTQRWVLKERMTGMDTNGDGKLEYKE 234

Query: 181 FLHHTYDIYKSYIDFE-TQGEDVPTAEEKFDELDLDKDEFLSVEELSPLFQYLHPGELSY 240
           F+ + Y++YK +  FE  + E+VPT +  F E+D DKD FL  +EL P+ QYL PGE+SY
Sbjct: 235 FVKNAYEMYKEFAKFEKEEDENVPTPQLLFAEMDRDKDRFLVADELRPILQYLQPGEMSY 294

Query: 241 AEHYASHLINEADDNKDGYLTLDEMLNHEFVFYTTVYEDRNGDYEDDYHDEL 291
           A+ Y++ L +EAD++KDG L+L+EML+HE VFY  V+ +   D +   HDEL
Sbjct: 295 AKFYSTFLCHEADEDKDGKLSLEEMLHHEDVFYKAVHHEDLDDEDYFDHDEL 345

[Close]

BLAST of Lag0020144 vs. TAIR 10
Match: AT5G08580.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 199.9 bits (507), Expect = 2.8e-51
Identity = 104/272 (38.24%), Postives = 167/272 (61.40%), Query Frame = 0


Query: 25  DAYKHYFDEGILNITLRLMVLFPLLDRSPKDGAISYDELNDWITGQAMERLNYRTRKQLA 84
           DA  +  DE   N+T RL++LFP +D SP DG ++  EL +W    + + + +RT++ L 
Sbjct: 119 DAEDYLNDEEKFNVTDRLILLFPKIDVSPADGFMTESELTEWTMQSSAKEVVHRTQRDLD 178

Query: 85  YYDKNGDGAISFNEYLPQFTEEDIAINATGHGEAGWWKKQFTNA-DLDPNGLLNFDELKD 144
            +D+N DG ISF+EY P         N+ G+ + GWWK++  NA D + +GLLN  E  D
Sbjct: 179 VHDRNKDGFISFSEYEPPSWVRKSDNNSFGY-DMGWWKEEHFNASDANGDGLLNLTEFND 238

Query: 145 FLHPEDSSNYRIQNWLLREKIKRMDSDRDGKLNFDEFLHHTYDIYKSYIDFETQG----E 204
           FLHP D+ N ++  WL +E+++  DSD+DGK++F+EF H  +D  ++Y +          
Sbjct: 239 FLHPADTKNPKLLLWLCKEEVRERDSDKDGKISFEEFFHGLFDTVRNYEEDNHNSTHPYH 298

Query: 205 DVP--TAEEKFDELDLDKDEFLSVEELSPLFQYLHPGELSYAEHYASHLINEADDNKDGY 264
           D+P   A++ F +LD + D +LS  EL P+   +HP E  YA+  A ++I++AD +KD  
Sbjct: 299 DLPEGPAKQLFSQLDKNDDGYLSDVELLPIISKIHPTEHYYAKQQADYIISQADSDKDRR 358

Query: 265 LTLDEMLNHEFVFYTTVYEDRNGDYEDDYHDE 290
           LTL EM+ H +VFY+ ++++ + D +  +HDE
Sbjct: 359 LTLAEMIEHPYVFYSAIFDEDDTDDDYGFHDE 389

[Close]

BLAST of Lag0020144 vs. TAIR 10
Match: AT3G50360.1 (centrin2 )

HSP 1 Score: 59.3 bits (142), Expect = 5.9e-09
Identity = 44/140 (31.43%), Postives = 65/140 (46.43%), Query Frame = 0


Query: 46  FPLLDRSPKDGAISYDELNDWITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYLPQFT- 105
           F L D +   G I   ELN  +     E    +  K +A  DK+G GAI F+E++   T 
Sbjct: 32  FELFD-TDGSGTIDAKELNVAMRALGFEMTEEQINKMIADVDKDGSGAIDFDEFVHMMTA 91

Query: 106 ---EEDIAINATGHGEAGWWKKQFTNADLDPNGLLNFDELKDFLHPEDSSNYRIQNWLLR 165
              E D     T         K F   DLD NG ++ D++K  +  +   N+      +R
Sbjct: 92  KIGERDTKEELT---------KAFQIIDLDKNGKISPDDIKR-MAKDLGENFTDAE--IR 151

Query: 166 EKIKRMDSDRDGKLNFDEFL 182
           E ++  D DRDG++N DEF+
Sbjct: 152 EMVEEADRDRDGEVNMDEFM 158

[Close]

BLAST of Lag0020144 vs. TAIR 10
Match: AT4G37010.1 (centrin 2 )

HSP 1 Score: 52.0 bits (123), Expect = 9.4e-07
Identity = 48/178 (26.97%), Postives = 76/178 (42.70%), Query Frame = 0


Query: 7   KRELESEGDAIGVGNQVPDAYKHYFDEGILNITLRLMVLFPLLDRSPKDGAISYDELNDW 66
           +R L+ +G   G+ NQ     +  FD            LF +       G+I   ELN  
Sbjct: 8   RRGLKPKGKTYGLTNQKRREIREIFD------------LFDI----DGSGSIDASELNVA 67

Query: 67  ITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYLPQFTEEDIAINATGHGE---AGWWKK 126
           +     E  N +  + +A  DKN  GAI F+E++   T        T  GE        K
Sbjct: 68  MRSLGFEMNNQQINELMAEVDKNQSGAIDFDEFVHMMT--------TKFGERDSIDELSK 127

Query: 127 QFTNADLDPNGLLNFDELKDFLHPEDSSNYRIQNWLLREKIKRMDSDRDGKLNFDEFL 182
            F   D D NG ++  ++K  +  E   N+   +  + E I+  D D+DG++N +EF+
Sbjct: 128 AFKIIDHDNNGKISPRDIK-MIAKELGENFTDND--IEEMIEEADRDKDGEVNLEEFM 158

[Close]

BLAST of Lag0020144 vs. TAIR 10
Match: AT4G37010.2 (centrin 2 )

HSP 1 Score: 52.0 bits (123), Expect = 9.4e-07
Identity = 48/178 (26.97%), Postives = 76/178 (42.70%), Query Frame = 0


Query: 7   KRELESEGDAIGVGNQVPDAYKHYFDEGILNITLRLMVLFPLLDRSPKDGAISYDELNDW 66
           +R L+ +G   G+ NQ     +  FD            LF +       G+I   ELN  
Sbjct: 12  RRGLKPKGKTYGLTNQKRREIREIFD------------LFDI----DGSGSIDASELNVA 71

Query: 67  ITGQAMERLNYRTRKQLAYYDKNGDGAISFNEYLPQFTEEDIAINATGHGE---AGWWKK 126
           +     E  N +  + +A  DKN  GAI F+E++   T        T  GE        K
Sbjct: 72  MRSLGFEMNNQQINELMAEVDKNQSGAIDFDEFVHMMT--------TKFGERDSIDELSK 131

Query: 127 QFTNADLDPNGLLNFDELKDFLHPEDSSNYRIQNWLLREKIKRMDSDRDGKLNFDEFL 182
            F   D D NG ++  ++K  +  E   N+   +  + E I+  D D+DG++N +EF+
Sbjct: 132 AFKIIDHDNNGKISPRDIK-MIAKELGENFTDND--IEEMIEEADRDKDGEVNLEEFM 162

The following BLAST results are available for this feature:

BLAST of Lag0020144 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011651314.1	1.9e-134	79.52	reticulocalbin-2 [Cucumis sativus] >KGN57652.1 hypothetical protein Csa_010748 [...	[more]
XP_022141310.1	3.2e-134	79.32	reticulocalbin-2 [Momordica charantia]	[more]
XP_008455987.1	1.4e-132	78.97	PREDICTED: reticulocalbin-2 [Cucumis melo] >KAA0050524.1 reticulocalbin-2 [Cucum...	[more]
TYK11288.1	6.7e-132	78.62	reticulocalbin-2 [Cucumis melo var. makuwa]	[more]
XP_038880351.1	3.7e-130	78.28	calumenin-B [Benincasa hispida]	[more]

BLAST of Lag0020144 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8BP92	1.3e-16	28.52	Reticulocalbin-2 OS=Mus musculus OX=10090 GN=Rcn2 PE=1 SV=1	[more]
J3S9D9	6.3e-16	28.14	Reticulocalbin-2 OS=Crotalus adamanteus OX=8729 PE=1 SV=1	[more]
Q62703	6.3e-16	28.14	Reticulocalbin-2 OS=Rattus norvegicus OX=10116 GN=Rcn2 PE=1 SV=2	[more]
B5X4E0	2.7e-14	27.97	Calumenin-B OS=Salmo salar OX=8030 GN=calub PE=2 SV=1	[more]
Q14257	4.5e-14	26.67	Reticulocalbin-2 OS=Homo sapiens OX=9606 GN=RCN2 PE=1 SV=1	[more]

BLAST of Lag0020144 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LAH3	9.2e-135	79.52	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G238180 PE=4 SV=1	[more]
A0A6J1CHQ2	1.6e-134	79.32	reticulocalbin-2 OS=Momordica charantia OX=3673 GN=LOC111011736 PE=4 SV=1	[more]
A0A5A7U5L5	6.6e-133	78.97	Reticulocalbin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G001...	[more]
A0A1S3C255	6.6e-133	78.97	reticulocalbin-2 OS=Cucumis melo OX=3656 GN=LOC103496051 PE=4 SV=1	[more]
A0A5D3CJD2	3.3e-132	78.62	Reticulocalbin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G001...	[more]

BLAST of Lag0020144 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27790.1	1.2e-73	46.92	Calcium-binding EF hand family protein	[more]
AT5G08580.1	2.8e-51	38.24	Calcium-binding EF hand family protein	[more]
AT3G50360.1	5.9e-09	31.43	centrin2	[more]
AT4G37010.1	9.4e-07	26.97	centrin 2	[more]
AT4G37010.2	9.4e-07	26.97	centrin 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 120..148 e-value: 2.8 score: 14.1 coord: 159..187 e-value: 0.27 score: 20.3 coord: 242..270 e-value: 44.0 score: 4.5 coord: 41..70 e-value: 150.0 score: 0.3 coord: 203..231 e-value: 11.0 score: 9.3 coord: 78..106 e-value: 12.0 score: 9.0
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 165..180 e-value: 0.015 score: 14.7 coord: 85..98 e-value: 0.0086 score: 15.5
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 205..264 e-value: 5.4E-9 score: 36.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 160..190 score: 9.157488
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 199..234 score: 10.3012
None	No IPR available	GENE3D	1.10.238.10		coord: 40..101 e-value: 3.2E-7 score: 32.4
None	No IPR available	GENE3D	1.10.238.10		coord: 102..185 e-value: 2.3E-10 score: 42.7
None	No IPR available	GENE3D	1.10.238.10		coord: 186..268 e-value: 1.6E-11 score: 46.3
None	No IPR available	PANTHER	PTHR10827:SF77	CALCIUM-BINDING EF HAND FAMILY PROTEIN	coord: 1..290
None	No IPR available	PANTHER	PTHR10827	RETICULOCALBIN	coord: 1..290
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 251..263
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 129..141
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 168..180
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 87..99
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 118..282
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 41..101

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0020144.1	Lag0020144.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding