Lag0020122 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0020122
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Pectinesterase
Location	chr5: 48276947 .. 48279826 (+)
RNA-Seq Expression	Lag0020122
Synteny	Lag0020122

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCTACGGCAGGCTCAACGAAACCGACCCCGGCGGCACCTCTGGTGCCGTCACCTTCCAACTCTCCGACCACGCCGTTTCCTCTCCCTCTCCCAACCGGAGGCGGAGGCGACTTCTCCTTCTCTCCCTAATGTCCCTCATTCTCATCGTCGCCGCCGCCGTCTCCGCCACCTTCCTCGTCGCCCTCCGAACCGCCTCCCGCGCCCGATCCGACCCGAACCTCCCTCGAAAACCGACTCAGGCCATTTCTAGGGCTTGTAGCCGAACTCGCTTCCCGACTCTCTGTGTCAACTCGCTCCTCGATTTCCCTGAATCTCTCAACGCCAACGAGCAGGATCTCGTCCACATTTCCTTCAATTTGACTCTCCACCATCTCAACCGAGCGCTCTATTTGTCCTCTGAGATCTCCTCTCTTCAAATGGATCTTCGCGTCCGCTCCGCCTACGACGCTTGTCTCGAACTCCTCGACGACTCTGTCGACGCTCTCACTCGCTCCTTGCAGTCCGTCGCGCCGCCTTCCTCCGACGCCGCCCCGCAGCGACTCGGCTCCTCTCAGGACGTCGTCACCTGGCTCAGTGCGGCTCTCACGAATCAAGATACCTGTACGGATGGATTCTCTGAACTCAGCGGGAGTGTCAAAGATCAAATGACGGATAAACTCCACAATTTGTCGGAGCTTGTGAGCAATTGCTTGGCGCTGTTTTCCGGTGGCGAAACGAGCGATTTCTCCGGAGTGCCGATTCAGAACAAGCGACGGTTGATGAAGGCCGGCGAGAAGGAGGATAGTTCCGGTGACTTCCCCGGCTGGATGAGTCGGCGGGAGAGGAGGCTGCTGACGCTGCCGGTGGGAGCGCTGCAGGCCGATATTGTGGTTTCGAAGGACGGGAACGGAACGGTGAAGACGATCGCCGAAGCGATCAAAAAGGCGCCGGAATACAGTAGCAAAAGGACGATCATCTATGTAATGGCAGGAAGGTAAATTTATATCGCTGGTTTATTCCTAAACTTTCCAATTTTAAATAGCTTTTATCAATTAATTTTATGTTTAAAGAAAGTTTTAAACTGATTTTTTAAAATTAAGTTATCAATCTTCTACTTGTTTTAGAAAAACAAAAATAATTTTCAAAAACAAAAAAACAAATAGATATCAAACAACACTTAAAAAATTTTAATTTCATTTATTTTTTAGATTTATAATGTATAAATTAACAAATTTATTAAAGACAAAATTAATTTGGTTGATTTAATTTTCAACTGCAACCGCGTTTCGTTTGACTTGCGTGTCACGATCGTGAACTGTTTTCACGTGATTGGAAAAAGTACGCGCCATACGAATTTGGGAAAGTGGATTTACGGAAATGCCCTGCTTTCGTCCTTTCTGTTTCTGCAGATACGAAGAGAAGAATCTCAAGGTAGGGAGGAAGAAAACGAACCTGATGTTTGTTGGAGATGGCAAGGGCAAGACTGTCATTTCCGGTGCTAAGAGCATCTTCGACAACGTGACAACTTTTCACACGGCGTCGTTTGGTAATAAATCTCTTTCATCTAACTCTCCTCAATTATTTAACTTTTACTTATTCTTTTTGACCACAATAATTTATGGGGTCAGAATAATTCCTCTGTTAAATTAAAAAAAAAAAAAAAAAAAAAAAAACCCACGTGCAAATGCATCCTTGATTGAACTCGCACGTGCCATCTCATCATCTTCTTGTTCCTCTTGTCGGATTTTCTCAATTAGACTAAATTACCCTTCTATTACGATGAGAAATTATTAAGTGTTTTCGATGCAACGTTTCAAGTAGGTTTACACCATCAAATGTAAAAGAAAAAAATTGTAAAAGTACGTTGATTTTTATTAAACAACAGACAAATTTAAAAATATATATTTGGTGGTTCGTTGTACTGAACGCACCTAATAATTTGGCTAATTATTGCACCTCTCTTGGAAAAACATACTCTCTATGGGCAAGAAGTGGAGGTCACAGTCACATGCCACAATTTCAGAGAAATATTTTACACCATTTCTCTAATTAAATTATCCAATTCATATTGTCAAAAAAAAAATTAATTAATTAATTATCCAATTAATTACTATAATAATTCCCGCCATTTGGATATACACTATTTAAATAATATTGGACCTATCTGATCTGATTTCTTTTGAAAATTTTTCTTTACAGCGGCCACCGGCGCCGGCTTAATCTTGAGGGACATGACATTTGAGAATTGGGCTGGACCAGGGCGGCACCAGGCGGTGGCGCTCCGAGTAGGAGCGGACCACGCCGTCGTGTACCGGTGCAACATAATCGGCTATCAAGACACTCTGTACGTCCATTCGAACCGCCAATTCTACCGAGAGTGCGACATTTACGGCACCGTGGATTTCATCTTCGGCAATGCGGCGGTGGTGTTTCAGAACTGCAGCATCTACGCCCGAAAGGCGATGGACCTTCAAAAGAACACGATCACGGCCCAAAACCGGAAGGATCCGAATCAGAACACCGGAATTTCCATCCATGCTTGCCGGATTCTGGCCGCCTCCGATTTGGCCTCCTCCAACAGCAGCAACCCGACTTATCTGGGCCGTCCGTGGAAATTATACTCTAGAGTCGTTTACATGCTTTCGTACATAGGCGGCCACGTGCACCCACGCGGTTGGCTGGAGTGGAACGCCACGTTTGCACTTGACACGTTGTATTACGGTGAGTATATGAATTCCGGGCCAGGTGGGGCTGTGGGCCAGAGGGTGACGTGGCCCGGGTATCGGGTCATAACGTCCACGGTGGAGGCGAGTAAGTTCACGGTGGCGCAGTTCATTTACGGGTCGTCGTGGTTGCCGTCGACTGGAGTCGCGTTTCTCGGAGGGCTAAATGTGTAA

mRNA sequence

ATGGGCTACGGCAGGCTCAACGAAACCGACCCCGGCGGCACCTCTGGTGCCGTCACCTTCCAACTCTCCGACCACGCCGTTTCCTCTCCCTCTCCCAACCGGAGGCGGAGGCGACTTCTCCTTCTCTCCCTAATGTCCCTCATTCTCATCGTCGCCGCCGCCGTCTCCGCCACCTTCCTCGTCGCCCTCCGAACCGCCTCCCGCGCCCGATCCGACCCGAACCTCCCTCGAAAACCGACTCAGGCCATTTCTAGGGCTTGTAGCCGAACTCGCTTCCCGACTCTCTGTGTCAACTCGCTCCTCGATTTCCCTGAATCTCTCAACGCCAACGAGCAGGATCTCGTCCACATTTCCTTCAATTTGACTCTCCACCATCTCAACCGAGCGCTCTATTTGTCCTCTGAGATCTCCTCTCTTCAAATGGATCTTCGCGTCCGCTCCGCCTACGACGCTTGTCTCGAACTCCTCGACGACTCTGTCGACGCTCTCACTCGCTCCTTGCAGTCCGTCGCGCCGCCTTCCTCCGACGCCGCCCCGCAGCGACTCGGCTCCTCTCAGGACGTCGTCACCTGGCTCAGTGCGGCTCTCACGAATCAAGATACCTGTACGGATGGATTCTCTGAACTCAGCGGGAGTGTCAAAGATCAAATGACGGATAAACTCCACAATTTGTCGGAGCTTGTGAGCAATTGCTTGGCGCTGTTTTCCGGTGGCGAAACGAGCGATTTCTCCGGAGTGCCGATTCAGAACAAGCGACGGTTGATGAAGGCCGGCGAGAAGGAGGATAGTTCCGGTGACTTCCCCGGCTGGATGAGTCGGCGGGAGAGGAGGCTGCTGACGCTGCCGGTGGGAGCGCTGCAGGCCGATATTGTGGTTTCGAAGGACGGGAACGGAACGGTGAAGACGATCGCCGAAGCGATCAAAAAGGCGCCGGAATACAGTAGCAAAAGGACGATCATCTATGTAATGGCAGGAAGATACGAAGAGAAGAATCTCAAGGTAGGGAGGAAGAAAACGAACCTGATGTTTGTTGGAGATGGCAAGGGCAAGACTGTCATTTCCGGTGCTAAGAGCATCTTCGACAACGTGACAACTTTTCACACGGCGTCGTTTGCGGCCACCGGCGCCGGCTTAATCTTGAGGGACATGACATTTGAGAATTGGGCTGGACCAGGGCGGCACCAGGCGGTGGCGCTCCGAGTAGGAGCGGACCACGCCGTCGTGTACCGGTGCAACATAATCGGCTATCAAGACACTCTGTACGTCCATTCGAACCGCCAATTCTACCGAGAGTGCGACATTTACGGCACCGTGGATTTCATCTTCGGCAATGCGGCGGTGGTGTTTCAGAACTGCAGCATCTACGCCCGAAAGGCGATGGACCTTCAAAAGAACACGATCACGGCCCAAAACCGGAAGGATCCGAATCAGAACACCGGAATTTCCATCCATGCTTGCCGGATTCTGGCCGCCTCCGATTTGGCCTCCTCCAACAGCAGCAACCCGACTTATCTGGGCCGTCCGTGGAAATTATACTCTAGAGTCGTTTACATGCTTTCGTACATAGGCGGCCACGTGCACCCACGCGGTTGGCTGGAGTGGAACGCCACGTTTGCACTTGACACGTTGTATTACGGTGAGTATATGAATTCCGGGCCAGGTGGGGCTGTGGGCCAGAGGGTGACGTGGCCCGGGTATCGGGTCATAACGTCCACGGTGGAGGCGAGTAAGTTCACGGTGGCGCAGTTCATTTACGGGTCGTCGTGGTTGCCGTCGACTGGAGTCGCGTTTCTCGGAGGGCTAAATGTGTAA

Coding sequence (CDS)

ATGGGCTACGGCAGGCTCAACGAAACCGACCCCGGCGGCACCTCTGGTGCCGTCACCTTCCAACTCTCCGACCACGCCGTTTCCTCTCCCTCTCCCAACCGGAGGCGGAGGCGACTTCTCCTTCTCTCCCTAATGTCCCTCATTCTCATCGTCGCCGCCGCCGTCTCCGCCACCTTCCTCGTCGCCCTCCGAACCGCCTCCCGCGCCCGATCCGACCCGAACCTCCCTCGAAAACCGACTCAGGCCATTTCTAGGGCTTGTAGCCGAACTCGCTTCCCGACTCTCTGTGTCAACTCGCTCCTCGATTTCCCTGAATCTCTCAACGCCAACGAGCAGGATCTCGTCCACATTTCCTTCAATTTGACTCTCCACCATCTCAACCGAGCGCTCTATTTGTCCTCTGAGATCTCCTCTCTTCAAATGGATCTTCGCGTCCGCTCCGCCTACGACGCTTGTCTCGAACTCCTCGACGACTCTGTCGACGCTCTCACTCGCTCCTTGCAGTCCGTCGCGCCGCCTTCCTCCGACGCCGCCCCGCAGCGACTCGGCTCCTCTCAGGACGTCGTCACCTGGCTCAGTGCGGCTCTCACGAATCAAGATACCTGTACGGATGGATTCTCTGAACTCAGCGGGAGTGTCAAAGATCAAATGACGGATAAACTCCACAATTTGTCGGAGCTTGTGAGCAATTGCTTGGCGCTGTTTTCCGGTGGCGAAACGAGCGATTTCTCCGGAGTGCCGATTCAGAACAAGCGACGGTTGATGAAGGCCGGCGAGAAGGAGGATAGTTCCGGTGACTTCCCCGGCTGGATGAGTCGGCGGGAGAGGAGGCTGCTGACGCTGCCGGTGGGAGCGCTGCAGGCCGATATTGTGGTTTCGAAGGACGGGAACGGAACGGTGAAGACGATCGCCGAAGCGATCAAAAAGGCGCCGGAATACAGTAGCAAAAGGACGATCATCTATGTAATGGCAGGAAGATACGAAGAGAAGAATCTCAAGGTAGGGAGGAAGAAAACGAACCTGATGTTTGTTGGAGATGGCAAGGGCAAGACTGTCATTTCCGGTGCTAAGAGCATCTTCGACAACGTGACAACTTTTCACACGGCGTCGTTTGCGGCCACCGGCGCCGGCTTAATCTTGAGGGACATGACATTTGAGAATTGGGCTGGACCAGGGCGGCACCAGGCGGTGGCGCTCCGAGTAGGAGCGGACCACGCCGTCGTGTACCGGTGCAACATAATCGGCTATCAAGACACTCTGTACGTCCATTCGAACCGCCAATTCTACCGAGAGTGCGACATTTACGGCACCGTGGATTTCATCTTCGGCAATGCGGCGGTGGTGTTTCAGAACTGCAGCATCTACGCCCGAAAGGCGATGGACCTTCAAAAGAACACGATCACGGCCCAAAACCGGAAGGATCCGAATCAGAACACCGGAATTTCCATCCATGCTTGCCGGATTCTGGCCGCCTCCGATTTGGCCTCCTCCAACAGCAGCAACCCGACTTATCTGGGCCGTCCGTGGAAATTATACTCTAGAGTCGTTTACATGCTTTCGTACATAGGCGGCCACGTGCACCCACGCGGTTGGCTGGAGTGGAACGCCACGTTTGCACTTGACACGTTGTATTACGGTGAGTATATGAATTCCGGGCCAGGTGGGGCTGTGGGCCAGAGGGTGACGTGGCCCGGGTATCGGGTCATAACGTCCACGGTGGAGGCGAGTAAGTTCACGGTGGCGCAGTTCATTTACGGGTCGTCGTGGTTGCCGTCGACTGGAGTCGCGTTTCTCGGAGGGCTAAATGTGTAA

Protein sequence

MGYGRLNETDPGGTSGAVTFQLSDHAVSSPSPNRRRRRLLLLSLMSLILIVAAAVSATFLVALRTASRARSDPNLPRKPTQAISRACSRTRFPTLCVNSLLDFPESLNANEQDLVHISFNLTLHHLNRALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSDAAPQRLGSSQDVVTWLSAALTNQDTCTDGFSELSGSVKDQMTDKLHNLSELVSNCLALFSGGETSDFSGVPIQNKRRLMKAGEKEDSSGDFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIFDNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPRGWLEWNATFALDTLYYGEYMNSGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV

Homology

[Close]

BLAST of Lag0020122 vs. NCBI nr
Match: XP_038881267.1 (probable pectinesterase/pectinesterase inhibitor 34 [Benincasa hispida])

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 552/603 (91.54%), Postives = 578/603 (95.85%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGAVTFQLSDHAVSSPSPNRRRRRLLLLSLMSLILIVAAAVSATFL 60
           MGYGRLNE+DPGGTS A  FQLSDH +S P+P+R R +L L+SL+SL+LIVAAAVSATFL
Sbjct: 1   MGYGRLNESDPGGTSSA-AFQLSDHPISFPTPSRSRAQLFLISLLSLLLIVAAAVSATFL 60

Query: 61  VALRTASRARSDPNLPRKPTQAISRACSRTRFPTLCVNSLLDFPESLNANEQDLVHISFN 120
           + +RTAS ARSD NLPRKPTQAIS+ACSRTRFP+LCVNSLLDFP SLNANEQDLVHISFN
Sbjct: 61  IGIRTASPARSDRNLPRKPTQAISKACSRTRFPSLCVNSLLDFPGSLNANEQDLVHISFN 120

Query: 121 LTLHHLNRALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSDAAPQ 180
           +TL HLN+ALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAP SS   PQ
Sbjct: 121 ITLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSSGTPQ 180

Query: 181 RLGSSQDVVTWLSAALTNQDTCTDGFSELSGSVKDQMTDKLHNLSELVSNCLALFSGGET 240
           RLGSS+DV+TWLSAALTNQDTCTDGFSELSG+VK+QM DKLHNLSELVSNCLALFSG ET
Sbjct: 181 RLGSSEDVITWLSAALTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFSGSET 240

Query: 241 SDFSGVPIQNKRRLMKAGEKEDSSGDFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTV 300
           SDFSGVPIQNKRRLM AG+ ED+SG+FPGWMSRRERRLLTLPVGALQADIVVS++GNGTV
Sbjct: 241 SDFSGVPIQNKRRLMNAGDNEDTSGNFPGWMSRRERRLLTLPVGALQADIVVSQNGNGTV 300

Query: 301 KTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIF 360
           KTIAEAIKKAP+YSS+RTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIF
Sbjct: 301 KTIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIF 360

Query: 361 DNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTL 420
           DNVTTFHTASFAATGAG+ILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTL
Sbjct: 361 DNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTL 420

Query: 421 YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTG 480
           YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAM LQKNTITAQNRKDPNQNTG
Sbjct: 421 YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMALQKNTITAQNRKDPNQNTG 480

Query: 481 ISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPRGWLEWNATFAL 540
           ISIHACRILA SDLASSNSSNPTYLGRPWKLYSRVVYMLSYIG HVHPRGWLEWNATFAL
Sbjct: 481 ISIHACRILATSDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGWLEWNATFAL 540

Query: 541 DTLYYGEYMNSGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG 600
           DTLYYGEYMN GPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG
Sbjct: 541 DTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG 600

Query: 601 LNV 604
           LNV
Sbjct: 601 LNV 602

[Close]

BLAST of Lag0020122 vs. NCBI nr
Match: XP_022984620.1 (probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita maxima])

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 551/603 (91.38%), Postives = 570/603 (94.53%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGAVTFQLSDHAVSSPSPNRRRRRLLLLSLMSLILIVAAAVSATFL 60
           M YGRLN+TDPGGTS     QLS HAV   SPN RRRRL+L+SL+SLILIVAAAVSATFL
Sbjct: 1   MAYGRLNQTDPGGTSSDAPSQLSHHAV---SPNPRRRRLILISLLSLILIVAAAVSATFL 60

Query: 61  VALRTASRARSDPNLPRKPTQAISRACSRTRFPTLCVNSLLDFPESLNANEQDLVHISFN 120
           V LRTAS  RSDPNLPRKPTQAIS+ACSRTRFPTLCVNSLLDFP SLNANEQDLVHISFN
Sbjct: 61  VGLRTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFN 120

Query: 121 LTLHHLNRALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSDAAPQ 180
           +TL HLN+ALY SS ISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAP SS+  PQ
Sbjct: 121 MTLQHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQ 180

Query: 181 RLGSSQDVVTWLSAALTNQDTCTDGFSELSGSVKDQMTDKLHNLSELVSNCLALFSGGET 240
           RLGSS DV+TWLSAALTNQDTCTDGFS+LSGSVKDQM DKLHNLSELVSNCLALFSG  T
Sbjct: 181 RLGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVT 240

Query: 241 SDFSGVPIQNKRRLMKAGEKEDSSGDFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTV 300
           SDFSGVPIQNKRRLM AGE ED+SG FPGWMSRRERRLLTLPVGALQADIVVS++G+GTV
Sbjct: 241 SDFSGVPIQNKRRLMNAGENEDTSGSFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTV 300

Query: 301 KTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIF 360
           KTIAEAIKKAP+YS++RTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISG KSIF
Sbjct: 301 KTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKCNLMFVGDGKGKTVISGGKSIF 360

Query: 361 DNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTL 420
           DNVTTFHTASFAATGAG+ILRDMTFENWAGPGRHQAVALRVG DHAVVYRCNIIGYQDTL
Sbjct: 361 DNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTL 420

Query: 421 YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTG 480
           YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTG
Sbjct: 421 YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTG 480

Query: 481 ISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPRGWLEWNATFAL 540
           ISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVV+MLSYIG HVHPRGWLEWNATFAL
Sbjct: 481 ISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFAL 540

Query: 541 DTLYYGEYMNSGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG 600
           DTLYYGEYMN GPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG
Sbjct: 541 DTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG 600

Query: 601 LNV 604
           LNV
Sbjct: 601 LNV 600

[Close]

BLAST of Lag0020122 vs. NCBI nr
Match: XP_023553011.1 (probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 550/603 (91.21%), Postives = 570/603 (94.53%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGAVTFQLSDHAVSSPSPNRRRRRLLLLSLMSLILIVAAAVSATFL 60
           MGYGRLNETDPGGTS      LS HA    SPN RRRRLLL+SL+SLILIV AAVSATFL
Sbjct: 1   MGYGRLNETDPGGTSSDAPSHLSHHAA---SPNPRRRRLLLISLLSLILIVTAAVSATFL 60

Query: 61  VALRTASRARSDPNLPRKPTQAISRACSRTRFPTLCVNSLLDFPESLNANEQDLVHISFN 120
           V LRTAS  RSDPNLPRKPTQAIS+ACSRTRFPTLCVNSLLDFP SLNANEQDLVHISFN
Sbjct: 61  VGLRTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFN 120

Query: 121 LTLHHLNRALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSDAAPQ 180
           +TL HLN+ALY SSEISSLQMDLRVRSAYDAC+ELLDDSVDALTRSLQSVAP SS+  PQ
Sbjct: 121 ITLQHLNKALYFSSEISSLQMDLRVRSAYDACVELLDDSVDALTRSLQSVAPSSSNGGPQ 180

Query: 181 RLGSSQDVVTWLSAALTNQDTCTDGFSELSGSVKDQMTDKLHNLSELVSNCLALFSGGET 240
           RLGSS DV+TWLSAALTNQDTCTDGFS+LSGSVKDQM DKLHNLSELVSNCLALFSG  T
Sbjct: 181 RLGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVT 240

Query: 241 SDFSGVPIQNKRRLMKAGEKEDSSGDFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTV 300
           SDFSGVPIQNKRRLM AG+ ED+SG+FPGWMSRRERRLLTLPVGALQADIVVS++G+GTV
Sbjct: 241 SDFSGVPIQNKRRLMNAGDDEDTSGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTV 300

Query: 301 KTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIF 360
           KTIAEAIKKAP+YS++RTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISG KSIF
Sbjct: 301 KTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIF 360

Query: 361 DNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTL 420
           DNVTTFHTASFAATGAG+ILRDMTFENWAGPGRHQAVALRVG DHAVVYRCNIIGYQDTL
Sbjct: 361 DNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTL 420

Query: 421 YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTG 480
           YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTG
Sbjct: 421 YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTG 480

Query: 481 ISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPRGWLEWNATFAL 540
           ISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVV+MLSYIG HVHPRGWLEWNATFAL
Sbjct: 481 ISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFAL 540

Query: 541 DTLYYGEYMNSGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG 600
           DTLYYGEYMN GPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG
Sbjct: 541 DTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG 600

Query: 601 LNV 604
           LNV
Sbjct: 601 LNV 600

[Close]

BLAST of Lag0020122 vs. NCBI nr
Match: XP_022922767.1 (probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita moschata])

HSP 1 Score: 1090.1 bits (2818), Expect = 0.0e+00
Identity = 550/603 (91.21%), Postives = 569/603 (94.36%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGAVTFQLSDHAVSSPSPNRRRRRLLLLSLMSLILIVAAAVSATFL 60
           MGYGRLNETDPGGTS      LS HA    SPN RR RLLL+SL+SLILIVAAAVSATFL
Sbjct: 1   MGYGRLNETDPGGTSSDAPSHLSHHAA---SPNPRRSRLLLISLLSLILIVAAAVSATFL 60

Query: 61  VALRTASRARSDPNLPRKPTQAISRACSRTRFPTLCVNSLLDFPESLNANEQDLVHISFN 120
           V LRTAS  RSDPNLPRKPTQAIS+ACSRTRFPTLCVNSLLDFP SLNANEQDLVHISFN
Sbjct: 61  VGLRTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFN 120

Query: 121 LTLHHLNRALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSDAAPQ 180
           +TL HLN+ALY SS ISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAP SS+  PQ
Sbjct: 121 MTLQHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQ 180

Query: 181 RLGSSQDVVTWLSAALTNQDTCTDGFSELSGSVKDQMTDKLHNLSELVSNCLALFSGGET 240
           RLGSS DV+TWLSAALTNQDTCTDGFS+LSGSVKDQM DKLHNLSELVSNCLALFSG  T
Sbjct: 181 RLGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVT 240

Query: 241 SDFSGVPIQNKRRLMKAGEKEDSSGDFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTV 300
           SDFSGVPIQNKRRLM AGE ED+SG+FPGWMSRRERRLLTLPVGALQADIVVS++G+GTV
Sbjct: 241 SDFSGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTV 300

Query: 301 KTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIF 360
           KTIAEAIKKAP+YS++RTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISG KSIF
Sbjct: 301 KTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIF 360

Query: 361 DNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTL 420
           DNVTTFHTASFAATGAG+ILRDMTFENWAGPGRHQAVALRVG DHAVVYRCNIIGYQDTL
Sbjct: 361 DNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTL 420

Query: 421 YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTG 480
           YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNT+TAQNRKDPNQNTG
Sbjct: 421 YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTG 480

Query: 481 ISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPRGWLEWNATFAL 540
           ISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVV+MLSYIG HVHPRGWLEWNATFAL
Sbjct: 481 ISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFAL 540

Query: 541 DTLYYGEYMNSGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG 600
           DTLYYGEYMN GPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG
Sbjct: 541 DTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG 600

Query: 601 LNV 604
           LNV
Sbjct: 601 LNV 600

[Close]

BLAST of Lag0020122 vs. NCBI nr
Match: KAA0050503.1 (pectin methylesterase family protein [Cucumis melo var. makuwa])

HSP 1 Score: 1072.0 bits (2771), Expect = 1.9e-309
Identity = 542/608 (89.14%), Postives = 571/608 (93.91%), Query Frame = 0


Query: 1   MGYGRLNETDPGG----TSGAVTFQLSDHAVSSPSPNRRRRRLLLLSLMSLILIVAAAVS 60
           MGYGRLNETDPGG    +S A  FQLS H    P+PNR   RLLL+S +SL+LIVAAAVS
Sbjct: 1   MGYGRLNETDPGGPSDSSSVASAFQLSAH---HPTPNRSTTRLLLISFLSLLLIVAAAVS 60

Query: 61  ATFLVALRTASRARSDPNLPRKPTQAISRACSRTRFPTLCVNSLLDFPESLNANEQDLVH 120
           ATFL+ LRTAS ARSDPNLPRKPTQAIS+ACSRTRFPTLCVNSLLDFP SLNANEQDLVH
Sbjct: 61  ATFLIGLRTASPARSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVH 120

Query: 121 ISFNLTLHHLNRALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSD 180
           ISFN+TL HLN+ALY SSEISSLQMDLRVRSAYDACLELLDDS+DA++RSLQSVAP SS 
Sbjct: 121 ISFNITLQHLNKALYSSSEISSLQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAPSSSS 180

Query: 181 AAPQRLGSSQDVVTWLSAALTNQDTCTDGFSELSGSVKDQMTDKLHNLSELVSNCLALFS 240
           + PQRLGSS+D++TWLSAALTNQDTCTDGFSELSG+VK+QM DKLHNLSELVSNCLALFS
Sbjct: 181 STPQRLGSSEDIITWLSAALTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFS 240

Query: 241 GGETSDFSGVPIQNKRRLMK-AGEKEDSSGDFPGWMSRRERRLLTLPVGALQADIVVSKD 300
           G ETSDF+GVPIQNKRRLMK  GE EDSSG+FP WM  RERRLLTLPVG LQADIVVS++
Sbjct: 241 GSETSDFAGVPIQNKRRLMKEEGENEDSSGNFPRWMRGRERRLLTLPVGVLQADIVVSQN 300

Query: 301 GNGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG 360
           GNGTVKTIAEAIKKAP+YS++RTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG
Sbjct: 301 GNGTVKTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG 360

Query: 361 AKSIFDNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIG 420
           +KSIFDNVTTFHTASFAATGAG+ILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIG
Sbjct: 361 SKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIG 420

Query: 421 YQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDP 480
           YQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAM LQKNTITAQNRKDP
Sbjct: 421 YQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMALQKNTITAQNRKDP 480

Query: 481 NQNTGISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPRGWLEWN 540
           NQNTGISIHACRILA SDL SSN+SNPTYLGRPWKLYSRVV+MLSYIGGHVHPRGWLEWN
Sbjct: 481 NQNTGISIHACRILATSDLQSSNTSNPTYLGRPWKLYSRVVFMLSYIGGHVHPRGWLEWN 540

Query: 541 ATFALDTLYYGEYMNSGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGV 600
           ATFALDTLYYGEYMN GPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGV
Sbjct: 541 ATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGV 600

Query: 601 AFLGGLNV 604
           AFLGGLNV
Sbjct: 601 AFLGGLNV 605

[Close]

BLAST of Lag0020122 vs. ExPASy Swiss-Prot
Match: Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 4.0e-230
Identity = 412/613 (67.21%), Postives = 482/613 (78.63%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGAVTFQ------LSDHAVSSPSPNRRRRRLLLLSLMSLILIVAAA 60
           MGY RL    P G +G+VT        L+  + S    N  RR    L + S++L ++  
Sbjct: 1   MGYERLG---PSGATGSVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLI 60

Query: 61  VSATFLVALRTASRA-RSDPNLPRKPTQAISRACSRTRFPTLCVNSLLDFPESLNA-NEQ 120
           ++A     +R+  +  +S P L RKP+QAIS+AC  TRFP LCV+SL+DFP SL A + +
Sbjct: 61  LAAAIFAGVRSRLKLNQSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSK 120

Query: 121 DLVHISFNLTLHHLNRALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAP 180
           DL+H++ N+TLHH + ALY S+ +S + M  R RSAYD+C+ELLDDSVDAL+R+L SV  
Sbjct: 121 DLIHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVV- 180

Query: 181 PSSDAAPQRLGSSQDVVTWLSAALTNQDTCTDGFSEL-SGSVKDQMTDKLHNLSELVSNC 240
            SS A P      QDV TWLSAALTN DTCT+GF  +  G VKD MT  L NLSELVSNC
Sbjct: 181 -SSSAKP------QDVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNC 240

Query: 241 LALFSGG-ETSDFSGVPIQNKRRLMKAGEKEDSSGDFPGWMSRRERRLLTLPVGALQADI 300
           LA+FS   +  DF+GVPIQN RRL+   E+E+    FP WM  +ER +L +PV  +QADI
Sbjct: 241 LAIFSASHDGDDFAGVPIQN-RRLLGVEEREEK---FPRWMRPKEREILEMPVSQIQADI 300

Query: 301 VVSKDGNGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGK 360
           +VSKDGNGT KTI+EAIKKAP+ S++R IIYV AGRYEE NLKVGRKK NLMFVGDGKGK
Sbjct: 301 IVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGK 360

Query: 361 TVISGAKSIFDNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYR 420
           TVISG KSIFDN+TTFHTASFAATGAG I RD+TFENWAGP +HQAVALR+GADHAV+YR
Sbjct: 361 TVISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYR 420

Query: 421 CNIIGYQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQ 480
           CNIIGYQDTLYVHSNRQF+RECDIYGTVDFIFGNAAVV QNCSIYARK MD QKNTITAQ
Sbjct: 421 CNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQ 480

Query: 481 NRKDPNQNTGISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPRG 540
           NRKDPNQNTGISIHA R+LAASDL ++N S  TYLGRPWKL+SR VYM+SYIGGHVH RG
Sbjct: 481 NRKDPNQNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRG 540

Query: 541 WLEWNATFALDTLYYGEYMNSGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWL 600
           WLEWN TFALDTLYYGEY+NSGPG  +GQRV+WPGYRVI ST EA++FTVA+FIYGSSWL
Sbjct: 541 WLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWL 598

Query: 601 PSTGVAFLGGLNV 604
           PSTGV+FL GL++
Sbjct: 601 PSTGVSFLAGLSI 598

[Close]

BLAST of Lag0020122 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 763.1 bits (1969), Expect = 2.4e-219
Identity = 390/605 (64.46%), Postives = 461/605 (76.20%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGAVTFQLSDHAVSSPSPNRRRRRLLLLSLMSLILIVAAAVSATFL 60
           MGY RL      G SG       D A S P   ++ +  L+  L +L ++V   V     
Sbjct: 1   MGYDRL------GPSGPSNPNQKDPATSLPELQKKTKTKLI--LFTLAVLVVGVVCFGIF 60

Query: 61  VALRTASRARSDPNLPRKPTQAISRACSRTRFPTLCVNSLLDFPESLNANEQDLVHISFN 120
             +R     +++P L RKPTQAISR CS++ +P LC+++LLDFP SL A+E +L+HISFN
Sbjct: 61  AGIRAVDSGKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFN 120

Query: 121 LTLHHLNRALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSDAAPQ 180
            TL   ++ALY SS I+  QM  RVRSAYD+CLELLDDSVDALTR+L SV   S D    
Sbjct: 121 ATLQKFSKALYTSSTITYTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDE--- 180

Query: 181 RLGSSQDVVTWLSAALTNQDTCTDGFSEL---SGSVKDQMTDKLHNLSELVSNCLALFSG 240
              S  DV+TWLS+A+TN DTCTDGF E+    G VKDQ+   + +LSE+VSNCLA+F+ 
Sbjct: 181 ---SHSDVMTWLSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFA- 240

Query: 241 GETSDFSGVPIQNKRRLMKAGEKEDSSGDFPGWMSRRERRLLTLPVGALQADIVVSKDGN 300
           G+  D SGVP+ N R+L+   E E    + P W+ R +R LL  P  A+QADI VSKDG+
Sbjct: 241 GKVKDLSGVPVVNNRKLLGTEETE----ELPNWLKREDRELLGTPTSAIQADITVSKDGS 300

Query: 301 GTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAK 360
           GT KTIAEAIKKAPE+SS+R +IYV AGRYEE+NLKVGRKKTNLMF+GDGKGKTVI+G K
Sbjct: 301 GTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGK 360

Query: 361 SIFDNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQ 420
           SI D++TTFHTA+FAATGAG I+RDMTFEN+AGP +HQAVALRVG DHAVVYRCNIIGYQ
Sbjct: 361 SIADDLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQ 420

Query: 421 DTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQ 480
           D LYVHSNRQF+REC+IYGTVDFIFGNAAV+ Q+C+IYARK M  QK TITAQNRKDPNQ
Sbjct: 421 DALYVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQ 480

Query: 481 NTGISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPRGWLEWNAT 540
           NTGISIHAC++LA  DL +S  S PTYLGRPWKLYSRVVYM+S +G H+ PRGWLEWN  
Sbjct: 481 NTGISIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGP 540

Query: 541 FALDTLYYGEYMNSGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAF 600
           FALD+LYYGEYMN G G  +GQRV WPGY VITSTVEASKFTVAQFI GSSWLPSTGV+F
Sbjct: 541 FALDSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSF 586

Query: 601 LGGLN 603
             GL+
Sbjct: 601 FSGLS 586

[Close]

BLAST of Lag0020122 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 4.0e-121
Identity = 242/573 (42.23%), Postives = 351/573 (61.26%), Query Frame = 0


Query: 35  RRRRLLLLSLMSLILIVAAAVSATFLVALRTASRARSDPNLPRKPTQAISRACSRTRFPT 94
           ++++ L L+L + +L+VAA +     +     SR  S  N        +  +CS TR+P 
Sbjct: 25  KKKKKLFLALFATLLVVAAVIG----IVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPD 84

Query: 95  LCVNSLLDFPESLN--ANEQDLVHISFNLTLHHLNRALY-LSSEISSLQMDLRVRSAYDA 154
           LC +++   PE+     +++D++ +S N+T   +    + +   +    +  R + A   
Sbjct: 85  LCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHD 144

Query: 155 CLELLDDSVDALTRSLQSVAP-PSSDAAPQRLGSSQDVVTWLSAALTNQDTCTDGFS--E 214
           CLE +D+++D L ++++ +   P+  +  Q    + D+ T +SAA+TNQ TC DGFS  +
Sbjct: 145 CLETIDETLDELHKAVEDLEEYPNKKSLSQH---ADDLKTLMSAAMTNQGTCLDGFSHDD 204

Query: 215 LSGSVKDQMTDKLHNLSELVSNCLALFSGGETSDFSGVPIQNKRRLMKAGEKEDSSGDFP 274
            +  V+D ++D   ++ ++ SN LA+      +D   +   N R+L+   E+  +   +P
Sbjct: 205 ANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLI---EETSTVDGWP 264

Query: 275 GWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYE 334
            W+S  +RRL  L   ++  ++VV+ DG+G  KT+A ++  AP+  +KR II + AG Y 
Sbjct: 265 AWLSTGDRRL--LQSSSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRIKAGVYR 324

Query: 335 EKNLKVGRKKTNLMFVGDGKGKTVISGAKSIFDNVTTFHTASFAATGAGLILRDMTFENW 394
           E N++V +K  N+MF+GDG+ +T+I+G++++ D  TTF +A+ A  G G + RD+TF+N 
Sbjct: 325 E-NVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDITFQNT 384

Query: 395 AGPGRHQAVALRVGADHAVVYRCNIIGYQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVV 454
           AGP +HQAVALRVGAD +  Y C+++ YQDTLYVHSNRQF+  C I GTVDFIFGNAA V
Sbjct: 385 AGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAV 444

Query: 455 FQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIHACRILAASDLASSNSSNPTYLGRP 514
            QNC I+ARK    QKN +TAQ R DPNQNTGI I   RI A SDL     S PTYLGRP
Sbjct: 445 LQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRP 504

Query: 515 WKLYSRVVYMLSYIGGHVHPRGWLEWNATFALDTLYYGEYMNSGPGGAVGQRVTWPGYRV 574
           WK YSR V M S I   +HP GW EW+  FAL+TL+YGE+ N+G G     RV W G+RV
Sbjct: 505 WKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRV 564

Query: 575 ITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGL 602
           ITS  EA  FT   FI GSSWL STG  F  GL
Sbjct: 565 ITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

[Close]

BLAST of Lag0020122 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 6.8e-121
Identity = 244/573 (42.58%), Postives = 349/573 (60.91%), Query Frame = 0


Query: 35  RRRRLLLLSLMSLILIVAAAVSATFLVALRTASRARSDPNLPRKPTQAISRACSRTRFPT 94
           ++++ L L+L + +L+VAA +     +     SR  S  N        +  +CS TR+P 
Sbjct: 25  KKKKKLFLALFATLLVVAAVIG----IVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPD 84

Query: 95  LCVNSLLDFPESLN--ANEQDLVHISFNLTLHHLNRALY-LSSEISSLQMDLRVRSAYDA 154
           LC +++   PE+     +++D++ +S N+T   +    + +   +    +  R + A   
Sbjct: 85  LCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHD 144

Query: 155 CLELLDDSVDALTRSLQSVAP-PSSDAAPQRLGSSQDVVTWLSAALTNQDTCTDGFS--E 214
           CLE +D+++D L ++++ +   P+  +  Q    + D+ T +SAA+TNQ TC DGFS  +
Sbjct: 145 CLETIDETLDELHKAVEDLEEYPNKKSLSQH---ADDLKTLMSAAMTNQGTCLDGFSHDD 204

Query: 215 LSGSVKDQMTDKLHNLSELVSNCLALFSGGETSDFSGVPIQNKRRLMKAGEKEDSSGDFP 274
            +  V+D ++D   ++ ++ SN LA+      +D   +   N R+L    E+  +   +P
Sbjct: 205 ANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLT---EETSTVDGWP 264

Query: 275 GWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYE 334
            W+S  +RRL  L   ++  + VV+ DG+G  KT+A A+  AP+  +KR II + AG Y 
Sbjct: 265 AWLSPGDRRL--LQSSSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIRIKAGVYR 324

Query: 335 EKNLKVGRKKTNLMFVGDGKGKTVISGAKSIFDNVTTFHTASFAATGAGLILRDMTFENW 394
           E N++V +K  N+MF+GDG+ +T+I+G++++ D  TTF +A+ A  G G + RD+TF+N 
Sbjct: 325 E-NVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARDITFQNT 384

Query: 395 AGPGRHQAVALRVGADHAVVYRCNIIGYQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVV 454
           AGP +HQAVALRVGAD +  Y C+++ YQDTLYVHSNRQF+  C I GTVDFIFGNAA V
Sbjct: 385 AGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAV 444

Query: 455 FQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIHACRILAASDLASSNSSNPTYLGRP 514
            QNC I+ARK    QKN +TAQ R DPNQNTGI I   RI A SDL     S PTYLGRP
Sbjct: 445 LQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRP 504

Query: 515 WKLYSRVVYMLSYIGGHVHPRGWLEWNATFALDTLYYGEYMNSGPGGAVGQRVTWPGYRV 574
           WK YSR V M S I   +HP GW EW+  FAL+TL+YGE+ NSG G     RV W G+RV
Sbjct: 505 WKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRV 564

Query: 575 ITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGL 602
           ITS  EA  FT   FI GSSWL STG  F  GL
Sbjct: 565 ITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

[Close]

BLAST of Lag0020122 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 432.6 bits (1111), Expect = 7.5e-120
Identity = 245/589 (41.60%), Postives = 351/589 (59.59%), Query Frame = 0


Query: 34  RRRRRLLLLSLMSLILIVAAAVSATFLVALRTASRARSDPNLPRKPTQAISRACSRTRFP 93
           ++ ++L+LLS    +L VAA    +       AS+A     L       +  +CS TR+P
Sbjct: 15  KKNKKLVLLSAAVALLFVAAVAGIS-----AGASKANEKRTLSPSSHAVLRSSCSSTRYP 74

Query: 94  TLCVNSLLDFPESLNANEQDLVHISFNLTLHHLNRALYLSSEISSLQMDL--RVRSAYDA 153
            LC+++++        +++D++  S NLT+  +    +   ++   +  L  R ++A   
Sbjct: 75  ELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHD 134

Query: 154 CLELLDDSVDALTRSLQSV-APPSSDAAPQRLGSSQDVVTWLSAALTNQDTCTDGFS--E 213
           CLE +D+++D L  +++ +   P+     +  G   D+ T +S+A+TNQ+TC DGFS  +
Sbjct: 135 CLETIDETLDELHETVEDLHLYPTKKTLREHAG---DLKTLISSAITNQETCLDGFSHDD 194

Query: 214 LSGSVKDQMTDKLHNLSELVSNCLALFSGGETSDFSGVP-----IQNKRRLMK------- 273
               V+  +     ++  + SN LA+      +D +          N R+L +       
Sbjct: 195 ADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTV 254

Query: 274 ----AGEKEDSSGDFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPE 333
               AG  E  S  +P W+S  +RRL  L    ++AD  V+ DG+GT KT+A A+  APE
Sbjct: 255 AVDIAGAGELDSEGWPTWLSAGDRRL--LQGSGVKADATVAADGSGTFKTVAAAVAAAPE 314

Query: 334 YSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIFDNVTTFHTASFA 393
            S+KR +I++ AG Y E N++V +KK N+MF+GDG+ +T+I+G++++ D  TTFH+A+ A
Sbjct: 315 NSNKRYVIHIKAGVYRE-NVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVA 374

Query: 394 ATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLYVHSNRQFYREC 453
           A G   + RD+TF+N AGP +HQAVALRVG+D +  Y C+++ YQDTLYVHSNRQF+ +C
Sbjct: 375 AVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKC 434

Query: 454 DIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIHACRILAAS 513
            I GTVDFIFGNAAVV Q+C I+AR+    QKN +TAQ R DPNQNTGI I  CRI A S
Sbjct: 435 LIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATS 494

Query: 514 DLASSNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPRGWLEWNATFALDTLYYGEYMNSG 573
           DL S   S PTYLGRPWK YS+ V M S I   + P GW EW  TFAL+TL Y EY N+G
Sbjct: 495 DLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTG 554

Query: 574 PGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGL 602
            G     RV W G++VIT+  EA K+T  QFI G  WL STG  F  GL
Sbjct: 555 AGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

[Close]

BLAST of Lag0020122 vs. ExPASy TrEMBL
Match: A0A6J1J930 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111482853 PE=3 SV=1)

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 551/603 (91.38%), Postives = 570/603 (94.53%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGAVTFQLSDHAVSSPSPNRRRRRLLLLSLMSLILIVAAAVSATFL 60
           M YGRLN+TDPGGTS     QLS HAV   SPN RRRRL+L+SL+SLILIVAAAVSATFL
Sbjct: 1   MAYGRLNQTDPGGTSSDAPSQLSHHAV---SPNPRRRRLILISLLSLILIVAAAVSATFL 60

Query: 61  VALRTASRARSDPNLPRKPTQAISRACSRTRFPTLCVNSLLDFPESLNANEQDLVHISFN 120
           V LRTAS  RSDPNLPRKPTQAIS+ACSRTRFPTLCVNSLLDFP SLNANEQDLVHISFN
Sbjct: 61  VGLRTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFN 120

Query: 121 LTLHHLNRALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSDAAPQ 180
           +TL HLN+ALY SS ISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAP SS+  PQ
Sbjct: 121 MTLQHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQ 180

Query: 181 RLGSSQDVVTWLSAALTNQDTCTDGFSELSGSVKDQMTDKLHNLSELVSNCLALFSGGET 240
           RLGSS DV+TWLSAALTNQDTCTDGFS+LSGSVKDQM DKLHNLSELVSNCLALFSG  T
Sbjct: 181 RLGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVT 240

Query: 241 SDFSGVPIQNKRRLMKAGEKEDSSGDFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTV 300
           SDFSGVPIQNKRRLM AGE ED+SG FPGWMSRRERRLLTLPVGALQADIVVS++G+GTV
Sbjct: 241 SDFSGVPIQNKRRLMNAGENEDTSGSFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTV 300

Query: 301 KTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIF 360
           KTIAEAIKKAP+YS++RTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISG KSIF
Sbjct: 301 KTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKCNLMFVGDGKGKTVISGGKSIF 360

Query: 361 DNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTL 420
           DNVTTFHTASFAATGAG+ILRDMTFENWAGPGRHQAVALRVG DHAVVYRCNIIGYQDTL
Sbjct: 361 DNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTL 420

Query: 421 YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTG 480
           YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTG
Sbjct: 421 YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTG 480

Query: 481 ISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPRGWLEWNATFAL 540
           ISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVV+MLSYIG HVHPRGWLEWNATFAL
Sbjct: 481 ISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFAL 540

Query: 541 DTLYYGEYMNSGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG 600
           DTLYYGEYMN GPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG
Sbjct: 541 DTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG 600

Query: 601 LNV 604
           LNV
Sbjct: 601 LNV 600

[Close]

BLAST of Lag0020122 vs. ExPASy TrEMBL
Match: A0A6J1E487 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430666 PE=3 SV=1)

HSP 1 Score: 1090.1 bits (2818), Expect = 0.0e+00
Identity = 550/603 (91.21%), Postives = 569/603 (94.36%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGAVTFQLSDHAVSSPSPNRRRRRLLLLSLMSLILIVAAAVSATFL 60
           MGYGRLNETDPGGTS      LS HA    SPN RR RLLL+SL+SLILIVAAAVSATFL
Sbjct: 1   MGYGRLNETDPGGTSSDAPSHLSHHAA---SPNPRRSRLLLISLLSLILIVAAAVSATFL 60

Query: 61  VALRTASRARSDPNLPRKPTQAISRACSRTRFPTLCVNSLLDFPESLNANEQDLVHISFN 120
           V LRTAS  RSDPNLPRKPTQAIS+ACSRTRFPTLCVNSLLDFP SLNANEQDLVHISFN
Sbjct: 61  VGLRTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFN 120

Query: 121 LTLHHLNRALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSDAAPQ 180
           +TL HLN+ALY SS ISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAP SS+  PQ
Sbjct: 121 MTLQHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQ 180

Query: 181 RLGSSQDVVTWLSAALTNQDTCTDGFSELSGSVKDQMTDKLHNLSELVSNCLALFSGGET 240
           RLGSS DV+TWLSAALTNQDTCTDGFS+LSGSVKDQM DKLHNLSELVSNCLALFSG  T
Sbjct: 181 RLGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVT 240

Query: 241 SDFSGVPIQNKRRLMKAGEKEDSSGDFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTV 300
           SDFSGVPIQNKRRLM AGE ED+SG+FPGWMSRRERRLLTLPVGALQADIVVS++G+GTV
Sbjct: 241 SDFSGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTV 300

Query: 301 KTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIF 360
           KTIAEAIKKAP+YS++RTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISG KSIF
Sbjct: 301 KTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIF 360

Query: 361 DNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTL 420
           DNVTTFHTASFAATGAG+ILRDMTFENWAGPGRHQAVALRVG DHAVVYRCNIIGYQDTL
Sbjct: 361 DNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTL 420

Query: 421 YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTG 480
           YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNT+TAQNRKDPNQNTG
Sbjct: 421 YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTG 480

Query: 481 ISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPRGWLEWNATFAL 540
           ISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVV+MLSYIG HVHPRGWLEWNATFAL
Sbjct: 481 ISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFAL 540

Query: 541 DTLYYGEYMNSGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG 600
           DTLYYGEYMN GPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG
Sbjct: 541 DTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG 600

Query: 601 LNV 604
           LNV
Sbjct: 601 LNV 600

[Close]

BLAST of Lag0020122 vs. ExPASy TrEMBL
Match: A0A5A7U3N7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G001450 PE=3 SV=1)

HSP 1 Score: 1072.0 bits (2771), Expect = 9.0e-310
Identity = 542/608 (89.14%), Postives = 571/608 (93.91%), Query Frame = 0


Query: 1   MGYGRLNETDPGG----TSGAVTFQLSDHAVSSPSPNRRRRRLLLLSLMSLILIVAAAVS 60
           MGYGRLNETDPGG    +S A  FQLS H    P+PNR   RLLL+S +SL+LIVAAAVS
Sbjct: 1   MGYGRLNETDPGGPSDSSSVASAFQLSAH---HPTPNRSTTRLLLISFLSLLLIVAAAVS 60

Query: 61  ATFLVALRTASRARSDPNLPRKPTQAISRACSRTRFPTLCVNSLLDFPESLNANEQDLVH 120
           ATFL+ LRTAS ARSDPNLPRKPTQAIS+ACSRTRFPTLCVNSLLDFP SLNANEQDLVH
Sbjct: 61  ATFLIGLRTASPARSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVH 120

Query: 121 ISFNLTLHHLNRALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSD 180
           ISFN+TL HLN+ALY SSEISSLQMDLRVRSAYDACLELLDDS+DA++RSLQSVAP SS 
Sbjct: 121 ISFNITLQHLNKALYSSSEISSLQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAPSSSS 180

Query: 181 AAPQRLGSSQDVVTWLSAALTNQDTCTDGFSELSGSVKDQMTDKLHNLSELVSNCLALFS 240
           + PQRLGSS+D++TWLSAALTNQDTCTDGFSELSG+VK+QM DKLHNLSELVSNCLALFS
Sbjct: 181 STPQRLGSSEDIITWLSAALTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFS 240

Query: 241 GGETSDFSGVPIQNKRRLMK-AGEKEDSSGDFPGWMSRRERRLLTLPVGALQADIVVSKD 300
           G ETSDF+GVPIQNKRRLMK  GE EDSSG+FP WM  RERRLLTLPVG LQADIVVS++
Sbjct: 241 GSETSDFAGVPIQNKRRLMKEEGENEDSSGNFPRWMRGRERRLLTLPVGVLQADIVVSQN 300

Query: 301 GNGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG 360
           GNGTVKTIAEAIKKAP+YS++RTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG
Sbjct: 301 GNGTVKTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG 360

Query: 361 AKSIFDNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIG 420
           +KSIFDNVTTFHTASFAATGAG+ILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIG
Sbjct: 361 SKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIG 420

Query: 421 YQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDP 480
           YQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAM LQKNTITAQNRKDP
Sbjct: 421 YQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMALQKNTITAQNRKDP 480

Query: 481 NQNTGISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPRGWLEWN 540
           NQNTGISIHACRILA SDL SSN+SNPTYLGRPWKLYSRVV+MLSYIGGHVHPRGWLEWN
Sbjct: 481 NQNTGISIHACRILATSDLQSSNTSNPTYLGRPWKLYSRVVFMLSYIGGHVHPRGWLEWN 540

Query: 541 ATFALDTLYYGEYMNSGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGV 600
           ATFALDTLYYGEYMN GPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGV
Sbjct: 541 ATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGV 600

Query: 601 AFLGGLNV 604
           AFLGGLNV
Sbjct: 601 AFLGGLNV 605

[Close]

BLAST of Lag0020122 vs. ExPASy TrEMBL
Match: A0A0A0L6S0 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G239880 PE=3 SV=1)

HSP 1 Score: 1070.5 bits (2767), Expect = 2.7e-309
Identity = 541/608 (88.98%), Postives = 569/608 (93.59%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTS----GAVTFQLSDHAVSSPSPNRRRRRLLLLSLMSLILIVAAAVS 60
           MGYGRLNETDPGG S     +  FQLS H    P+PNRR  RLLL+S +SL+LIVAAAVS
Sbjct: 1   MGYGRLNETDPGGPSHSSPASPPFQLSSH---HPTPNRRTTRLLLISFLSLLLIVAAAVS 60

Query: 61  ATFLVALRTASRARSDPNLPRKPTQAISRACSRTRFPTLCVNSLLDFPESLNANEQDLVH 120
           ATFL+ LRTAS ARSDPNLPRKPTQAIS+ACSRTRFPTLCVNSLLDFP SLNANEQDLVH
Sbjct: 61  ATFLIGLRTASPARSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVH 120

Query: 121 ISFNLTLHHLNRALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSD 180
           ISFN+TL HLN+ALY SSEISSLQMDLRVRSAYDACLELLDDS+DA++RSLQSVAP SS 
Sbjct: 121 ISFNVTLQHLNKALYSSSEISSLQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAPSSSS 180

Query: 181 AAPQRLGSSQDVVTWLSAALTNQDTCTDGFSELSGSVKDQMTDKLHNLSELVSNCLALFS 240
           + PQRLGSS+D++TWLSAALTNQDTCTDGFSELSG+VK+QM DKLHNLSELVSNCLALFS
Sbjct: 181 STPQRLGSSEDIITWLSAALTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFS 240

Query: 241 GGETSDFSGVPIQNKRRLM-KAGEKEDSSGDFPGWMSRRERRLLTLPVGALQADIVVSKD 300
           G ETSDF+GVPIQNKRRLM + GE EDSSG FP WM+RRERRLLTLPVG LQADIVVS++
Sbjct: 241 GSETSDFAGVPIQNKRRLMEEEGENEDSSGKFPRWMNRRERRLLTLPVGVLQADIVVSQN 300

Query: 301 GNGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG 360
           GNGTVKTIAEAIKKAP+YSS+RTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG
Sbjct: 301 GNGTVKTIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG 360

Query: 361 AKSIFDNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIG 420
           +KSIFDNVTTFHTASFAATGAG+ILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIG
Sbjct: 361 SKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIG 420

Query: 421 YQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDP 480
           YQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAM LQKNTITAQNRKDP
Sbjct: 421 YQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMALQKNTITAQNRKDP 480

Query: 481 NQNTGISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPRGWLEWN 540
           NQNTGISIHACRILA SDL SSN+SNPTYLGRPWKLYSR V MLSYIG HVHPRGWLEWN
Sbjct: 481 NQNTGISIHACRILATSDLESSNTSNPTYLGRPWKLYSRTVVMLSYIGNHVHPRGWLEWN 540

Query: 541 ATFALDTLYYGEYMNSGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGV 600
           ATFALDTLYYGEYMN GPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGV
Sbjct: 541 ATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGV 600

Query: 601 AFLGGLNV 604
           AFLGGLNV
Sbjct: 601 AFLGGLNV 605

[Close]

BLAST of Lag0020122 vs. ExPASy TrEMBL
Match: A0A6J1G0F6 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111449561 PE=3 SV=1)

HSP 1 Score: 967.2 bits (2499), Expect = 3.1e-278
Identity = 497/605 (82.15%), Postives = 534/605 (88.26%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGAVTFQLSDHAVSSPSPNRRRRRLLLLSLMSLILIVAAAVSATFL 60
           MG  RLNET                    P+ N RR  L +LSL+SLILI+  AVS T L
Sbjct: 1   MGCRRLNET-------------------HPAANPRRGPLSVLSLLSLILIL-PAVSPTSL 60

Query: 61  VALRTASRARSDPNLPRKPTQAISRACSRTRFPTLCVNSLLDFPESLNANEQDLVHISFN 120
           + LRT+    SDP LPRKPT+AIS+AC+R+R P LCV+SLLDFP SL+ANE+DLVHISFN
Sbjct: 61  LGLRTS----SDPKLPRKPTRAISKACARSRLPALCVDSLLDFPGSLSANERDLVHISFN 120

Query: 121 LTLHHLNRALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSDAAPQ 180
           LTLHHLN ALY SS +SSL MDL +RSAYDACLELLDDSV ALTRS  S+APPSSD+ P 
Sbjct: 121 LTLHHLNEALYSSSHLSSLHMDLPLRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPT 180

Query: 181 RL-GSSQDVVTWLSAALTNQDTCTDGFSELSGSVKDQMTDKLHNLSELVSNCLALFSGGE 240
            L  SS DV+TWLSAALTNQDTCTDGFS+LSG+VKDQMTDKLHNLSELVSNCLALFSG  
Sbjct: 181 PLASSSDDVITWLSAALTNQDTCTDGFSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSV 240

Query: 241 TSDFSGVPIQNKRRLMKAGEKEDSSGDFPGWMSRRERRLLTLPVGALQADIVVSKDGNGT 300
            +DFSGVPIQNKRRLM A EKED SG+FPGWMSRRERRLLTLPVGALQADIVVSKDGNGT
Sbjct: 241 RNDFSGVPIQNKRRLMNAAEKEDDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGT 300

Query: 301 VKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSI 360
           VKTIAEAIKKAPE+S+++TIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG KSI
Sbjct: 301 VKTIAEAIKKAPEFSTRKTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSI 360

Query: 361 FDNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDT 420
           FDNVTTFHTASFA +GAG+ILRDMTF NWAGP RHQAVALRV ADHAVVYRCN+IGYQDT
Sbjct: 361 FDNVTTFHTASFAVSGAGIILRDMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDT 420

Query: 421 LYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNT 480
           LYVHSNRQF+RECDIYGTVDFIFGNAAVVFQNCSIYARKAM+LQKNTITAQNRKDPNQNT
Sbjct: 421 LYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNT 480

Query: 481 GISIHACRILAASDLASSN-SSNPTYLGRPWKLYSRVVYMLSYIGGHVHPRGWLEWNATF 540
           GISIHACRILA  DLASS+ ++NPTYLGRPWKLYSRVVYM SY+GGHVHPRGWLEWNATF
Sbjct: 481 GISIHACRILATPDLASSSTATNPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATF 540

Query: 541 ALDTLYYGEYMNSGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFL 600
           ALDTLYYGEYMN GPGGAVGQRVTWPGYRVITS VEAS+FTVAQFIYGSSWLPSTGVAFL
Sbjct: 541 ALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFL 581

Query: 601 GGLNV 604
           GGLN+
Sbjct: 601 GGLNL 581

[Close]

BLAST of Lag0020122 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 798.9 bits (2062), Expect = 2.8e-231
Identity = 412/613 (67.21%), Postives = 482/613 (78.63%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGAVTFQ------LSDHAVSSPSPNRRRRRLLLLSLMSLILIVAAA 60
           MGY RL    P G +G+VT        L+  + S    N  RR    L + S++L ++  
Sbjct: 1   MGYERLG---PSGATGSVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLI 60

Query: 61  VSATFLVALRTASRA-RSDPNLPRKPTQAISRACSRTRFPTLCVNSLLDFPESLNA-NEQ 120
           ++A     +R+  +  +S P L RKP+QAIS+AC  TRFP LCV+SL+DFP SL A + +
Sbjct: 61  LAAAIFAGVRSRLKLNQSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSK 120

Query: 121 DLVHISFNLTLHHLNRALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAP 180
           DL+H++ N+TLHH + ALY S+ +S + M  R RSAYD+C+ELLDDSVDAL+R+L SV  
Sbjct: 121 DLIHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVV- 180

Query: 181 PSSDAAPQRLGSSQDVVTWLSAALTNQDTCTDGFSEL-SGSVKDQMTDKLHNLSELVSNC 240
            SS A P      QDV TWLSAALTN DTCT+GF  +  G VKD MT  L NLSELVSNC
Sbjct: 181 -SSSAKP------QDVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNC 240

Query: 241 LALFSGG-ETSDFSGVPIQNKRRLMKAGEKEDSSGDFPGWMSRRERRLLTLPVGALQADI 300
           LA+FS   +  DF+GVPIQN RRL+   E+E+    FP WM  +ER +L +PV  +QADI
Sbjct: 241 LAIFSASHDGDDFAGVPIQN-RRLLGVEEREEK---FPRWMRPKEREILEMPVSQIQADI 300

Query: 301 VVSKDGNGTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGK 360
           +VSKDGNGT KTI+EAIKKAP+ S++R IIYV AGRYEE NLKVGRKK NLMFVGDGKGK
Sbjct: 301 IVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGK 360

Query: 361 TVISGAKSIFDNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYR 420
           TVISG KSIFDN+TTFHTASFAATGAG I RD+TFENWAGP +HQAVALR+GADHAV+YR
Sbjct: 361 TVISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYR 420

Query: 421 CNIIGYQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQ 480
           CNIIGYQDTLYVHSNRQF+RECDIYGTVDFIFGNAAVV QNCSIYARK MD QKNTITAQ
Sbjct: 421 CNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQ 480

Query: 481 NRKDPNQNTGISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPRG 540
           NRKDPNQNTGISIHA R+LAASDL ++N S  TYLGRPWKL+SR VYM+SYIGGHVH RG
Sbjct: 481 NRKDPNQNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRG 540

Query: 541 WLEWNATFALDTLYYGEYMNSGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWL 600
           WLEWN TFALDTLYYGEY+NSGPG  +GQRV+WPGYRVI ST EA++FTVA+FIYGSSWL
Sbjct: 541 WLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWL 598

Query: 601 PSTGVAFLGGLNV 604
           PSTGV+FL GL++
Sbjct: 601 PSTGVSFLAGLSI 598

[Close]

BLAST of Lag0020122 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 763.1 bits (1969), Expect = 1.7e-220
Identity = 390/605 (64.46%), Postives = 461/605 (76.20%), Query Frame = 0


Query: 1   MGYGRLNETDPGGTSGAVTFQLSDHAVSSPSPNRRRRRLLLLSLMSLILIVAAAVSATFL 60
           MGY RL      G SG       D A S P   ++ +  L+  L +L ++V   V     
Sbjct: 1   MGYDRL------GPSGPSNPNQKDPATSLPELQKKTKTKLI--LFTLAVLVVGVVCFGIF 60

Query: 61  VALRTASRARSDPNLPRKPTQAISRACSRTRFPTLCVNSLLDFPESLNANEQDLVHISFN 120
             +R     +++P L RKPTQAISR CS++ +P LC+++LLDFP SL A+E +L+HISFN
Sbjct: 61  AGIRAVDSGKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFN 120

Query: 121 LTLHHLNRALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPPSSDAAPQ 180
            TL   ++ALY SS I+  QM  RVRSAYD+CLELLDDSVDALTR+L SV   S D    
Sbjct: 121 ATLQKFSKALYTSSTITYTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDE--- 180

Query: 181 RLGSSQDVVTWLSAALTNQDTCTDGFSEL---SGSVKDQMTDKLHNLSELVSNCLALFSG 240
              S  DV+TWLS+A+TN DTCTDGF E+    G VKDQ+   + +LSE+VSNCLA+F+ 
Sbjct: 181 ---SHSDVMTWLSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFA- 240

Query: 241 GETSDFSGVPIQNKRRLMKAGEKEDSSGDFPGWMSRRERRLLTLPVGALQADIVVSKDGN 300
           G+  D SGVP+ N R+L+   E E    + P W+ R +R LL  P  A+QADI VSKDG+
Sbjct: 241 GKVKDLSGVPVVNNRKLLGTEETE----ELPNWLKREDRELLGTPTSAIQADITVSKDGS 300

Query: 301 GTVKTIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAK 360
           GT KTIAEAIKKAPE+SS+R +IYV AGRYEE+NLKVGRKKTNLMF+GDGKGKTVI+G K
Sbjct: 301 GTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGK 360

Query: 361 SIFDNVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQ 420
           SI D++TTFHTA+FAATGAG I+RDMTFEN+AGP +HQAVALRVG DHAVVYRCNIIGYQ
Sbjct: 361 SIADDLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQ 420

Query: 421 DTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQ 480
           D LYVHSNRQF+REC+IYGTVDFIFGNAAV+ Q+C+IYARK M  QK TITAQNRKDPNQ
Sbjct: 421 DALYVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQ 480

Query: 481 NTGISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPRGWLEWNAT 540
           NTGISIHAC++LA  DL +S  S PTYLGRPWKLYSRVVYM+S +G H+ PRGWLEWN  
Sbjct: 481 NTGISIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGP 540

Query: 541 FALDTLYYGEYMNSGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAF 600
           FALD+LYYGEYMN G G  +GQRV WPGY VITSTVEASKFTVAQFI GSSWLPSTGV+F
Sbjct: 541 FALDSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSF 586

Query: 601 LGGLN 603
             GL+
Sbjct: 601 FSGLS 586

[Close]

BLAST of Lag0020122 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 432.6 bits (1111), Expect = 5.3e-121
Identity = 245/589 (41.60%), Postives = 351/589 (59.59%), Query Frame = 0


Query: 34  RRRRRLLLLSLMSLILIVAAAVSATFLVALRTASRARSDPNLPRKPTQAISRACSRTRFP 93
           ++ ++L+LLS    +L VAA    +       AS+A     L       +  +CS TR+P
Sbjct: 15  KKNKKLVLLSAAVALLFVAAVAGIS-----AGASKANEKRTLSPSSHAVLRSSCSSTRYP 74

Query: 94  TLCVNSLLDFPESLNANEQDLVHISFNLTLHHLNRALYLSSEISSLQMDL--RVRSAYDA 153
            LC+++++        +++D++  S NLT+  +    +   ++   +  L  R ++A   
Sbjct: 75  ELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHD 134

Query: 154 CLELLDDSVDALTRSLQSV-APPSSDAAPQRLGSSQDVVTWLSAALTNQDTCTDGFS--E 213
           CLE +D+++D L  +++ +   P+     +  G   D+ T +S+A+TNQ+TC DGFS  +
Sbjct: 135 CLETIDETLDELHETVEDLHLYPTKKTLREHAG---DLKTLISSAITNQETCLDGFSHDD 194

Query: 214 LSGSVKDQMTDKLHNLSELVSNCLALFSGGETSDFSGVP-----IQNKRRLMK------- 273
               V+  +     ++  + SN LA+      +D +          N R+L +       
Sbjct: 195 ADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTV 254

Query: 274 ----AGEKEDSSGDFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPE 333
               AG  E  S  +P W+S  +RRL  L    ++AD  V+ DG+GT KT+A A+  APE
Sbjct: 255 AVDIAGAGELDSEGWPTWLSAGDRRL--LQGSGVKADATVAADGSGTFKTVAAAVAAAPE 314

Query: 334 YSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIFDNVTTFHTASFA 393
            S+KR +I++ AG Y E N++V +KK N+MF+GDG+ +T+I+G++++ D  TTFH+A+ A
Sbjct: 315 NSNKRYVIHIKAGVYRE-NVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVA 374

Query: 394 ATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLYVHSNRQFYREC 453
           A G   + RD+TF+N AGP +HQAVALRVG+D +  Y C+++ YQDTLYVHSNRQF+ +C
Sbjct: 375 AVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKC 434

Query: 454 DIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIHACRILAAS 513
            I GTVDFIFGNAAVV Q+C I+AR+    QKN +TAQ R DPNQNTGI I  CRI A S
Sbjct: 435 LIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATS 494

Query: 514 DLASSNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPRGWLEWNATFALDTLYYGEYMNSG 573
           DL S   S PTYLGRPWK YS+ V M S I   + P GW EW  TFAL+TL Y EY N+G
Sbjct: 495 DLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTG 554

Query: 574 PGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGL 602
            G     RV W G++VIT+  EA K+T  QFI G  WL STG  F  GL
Sbjct: 555 AGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

[Close]

BLAST of Lag0020122 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 419.5 bits (1077), Expect = 4.7e-117
Identity = 223/480 (46.46%), Postives = 305/480 (63.54%), Query Frame = 0


Query: 126 LNRALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQ-SVAPPSSDAAPQRLGS 185
           + +A+ + S+        RV +A   C++LLD + + L+  +  S +P   D +   +GS
Sbjct: 58  ITKAVAIVSKFDKKAGKSRVSNAIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVGS 117

Query: 186 SQDVVTWLSAALTNQDTCTDGFSELSGSVKDQMTDKLHNLSELVSNCLALFSGGETSDFS 245
             D+ TW+SAAL+NQDTC DGF   +G +K  +   L  +   V N L +          
Sbjct: 118 --DLRTWISAALSNQDTCLDGFEGTNGIIKKIVAGGLSKVGTTVRNLLTM--------VH 177

Query: 246 GVPIQNKRRLMKA---GEKEDSSGDFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVK 305
             P + K + +KA    +       FP W+   +R+LL        AD VV+ DG G   
Sbjct: 178 SPPSKPKPKPIKAQTMTKAHSGFSKFPSWVKPGDRKLLQTD-NITVADAVVAADGTGNFT 237

Query: 306 TIAEAIKKAPEYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIFD 365
           TI++A+  AP+YS+KR +I+V  G Y E N+++ +KK N+M VGDG   TVI+G +S  D
Sbjct: 238 TISDAVLAAPDYSTKRYVIHVKRGVYVE-NVEIKKKKWNIMMVGDGIDATVITGNRSFID 297

Query: 366 NVTTFHTASFAATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLY 425
             TTF +A+FA +G G I RD+TF+N AGP +HQAVA+R   D  V YRC + GYQDTLY
Sbjct: 298 GWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLY 357

Query: 426 VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGI 485
            HS RQF+REC I GTVDFIFG+A  VFQ+C I A++ +  QKN+ITAQ RKDPN+ TG 
Sbjct: 358 AHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGF 417

Query: 486 SIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPRGWLEWNATFALD 545
           +I    I A +DL  + ++  TYLGRPWKLYSR V+M +Y+   ++P GWLEWN  FALD
Sbjct: 418 TIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALD 477

Query: 546 TLYYGEYMNSGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGL 602
           TLYYGEYMNSGPG ++ +RV WPGY V+ ++ EA+ FTV+Q I G+ WLPSTG+ F+ GL
Sbjct: 478 TLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

[Close]

BLAST of Lag0020122 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 416.4 bits (1069), Expect = 3.9e-116
Identity = 237/586 (40.44%), Postives = 346/586 (59.04%), Query Frame = 0


Query: 27  VSSPSPNRRRRRLLLLSLMSLILIVAAAVSATFLVALRTASRARSD--PNLPRKPTQAIS 86
           +S  S  +  ++L+L S    +L++A+ V     +A  T ++ ++     L       + 
Sbjct: 8   ISKFSDFKNNKKLILSSAAIALLLLASIVG----IAATTTNQNKNQKITTLSSTSHAILK 67

Query: 87  RACSRTRFPTLCVNSLLDFPESLNANEQDLVHISFNLTLHHLNRALYLSSEISSLQMDLR 146
             CS T +P LC +++         ++++++  S NLT   +    +   ++ + +  L 
Sbjct: 68  SVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLT 127

Query: 147 VR--SAYDACLELLDDSVDALTRSLQSVAPPSSDAAPQRLGSSQDVVTWLSAALTNQDTC 206
            R  +A   CLE +D+++D L  +++ +       + ++   + D+ T +S+A+TNQ TC
Sbjct: 128 PREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRK--HADDLKTLISSAITNQGTC 187

Query: 207 TDGFS--ELSGSVKDQMTDKLHNLSELVSNCLALFSGGETSDFSGVPIQ---------NK 266
            DGFS  +    V+  +     ++  + SN LA+      +D +   ++         N 
Sbjct: 188 LDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNN 247

Query: 267 RRLMKAGEKEDSSGDFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAP 326
           R+L +     DS G +P W+S  +RRL  L    ++AD  V+ DG+G   T+A A+  AP
Sbjct: 248 RKLKEVTGDLDSDG-WPKWLSVGDRRL--LQGSTIKADATVADDGSGDFTTVAAAVAAAP 307

Query: 327 EYSSKRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIFDNVTTFHTASF 386
           E S+KR +I++ AG Y E N++V +KKTN+MF+GDG+GKT+I+G++++ D  TTFH+A+ 
Sbjct: 308 EKSNKRFVIHIKAGVYRE-NVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATV 367

Query: 387 AATGAGLILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLYVHSNRQFYRE 446
           AA G   + RD+TF+N AGP +HQAVALRVG+D +  Y+C++  YQDTLYVHSNRQF+ +
Sbjct: 368 AAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVK 427

Query: 447 CDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISIHACRILAA 506
           C I GTVDFIFGNAA V Q+C I AR+    QKN +TAQ R DPNQNTGI I  CRI   
Sbjct: 428 CHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGT 487

Query: 507 SDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGGHVHPRGWLEWNATFALDTLYYGEYMNS 566
           SDL +   + PTYLGRPWK YSR V M S I   + P GW EW+ +FALDTL Y EY+N 
Sbjct: 488 SDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNR 547

Query: 567 GPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAF 598
           G G     RV W GY+VITS  EA  FT  QFI G  WL STG  F
Sbjct: 548 GGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

The following BLAST results are available for this feature:

BLAST of Lag0020122 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881267.1	0.0e+00	91.54	probable pectinesterase/pectinesterase inhibitor 34 [Benincasa hispida]	[more]
XP_022984620.1	0.0e+00	91.38	probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita maxima]	[more]
XP_023553011.1	0.0e+00	91.21	probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita pepo subsp. pepo]	[more]
XP_022922767.1	0.0e+00	91.21	probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita moschata]	[more]
KAA0050503.1	1.9e-309	89.14	pectin methylesterase family protein [Cucumis melo var. makuwa]	[more]

BLAST of Lag0020122 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M3B0	4.0e-230	67.21	Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3...	[more]
Q9FK05	2.4e-219	64.46	Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3...	[more]
P83948	4.0e-121	42.23	Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1	[more]
O04886	6.8e-121	42.58	Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1	[more]
O49006	7.5e-120	41.60	Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME...	[more]

BLAST of Lag0020122 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J930	0.0e+00	91.38	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111482853 PE=3 SV=1	[more]
A0A6J1E487	0.0e+00	91.21	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430666 PE=3 SV=1	[more]
A0A5A7U3N7	9.0e-310	89.14	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G00145...	[more]
A0A0A0L6S0	2.7e-309	88.98	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G239880 PE=3 SV=1	[more]
A0A6J1G0F6	3.1e-278	82.15	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111449561 PE=3 SV=1	[more]

BLAST of Lag0020122 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G49220.1	2.8e-231	67.21	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G53370.1	1.7e-220	64.46	pectin methylesterase PCR fragment F	[more]
AT3G14310.1	5.3e-121	41.60	pectin methylesterase 3	[more]
AT3G43270.1	4.7e-117	46.46	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G53830.1	3.9e-116	40.44	pectin methylesterase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 78..234 e-value: 4.7E-42 score: 155.7
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 82..234 e-value: 4.4E-32 score: 111.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 56..235 e-value: 4.9E-40 score: 134.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 79..237 e-value: 2.2E-36 score: 127.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 81..236
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 289..586 e-value: 6.4E-142 score: 471.6
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 283..601 e-value: 1.6E-116 score: 391.1
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 29..601
None	No IPR available	PANTHER	PTHR31707:SF222	PECTINESTERASE	coord: 29..601
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 86..236 e-value: 8.40595E-45 score: 154.518
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 434..443
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 285..599

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0020122.1	Lag0020122.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity