Lag0020043 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0020043
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionFerric reduction oxidase
Locationchr5: 47594164 .. 47597822 (-)
RNA-Seq ExpressionLag0020043
SyntenyLag0020043
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAATGGCTAGAGCTCTAAAGATGATTTTCTTCTTAACATTTGTGCTTTGGCTGTGGCTTTGGATTATGTTGCCCACAAAGCTTTACAAGAACACTTGGACCCCTGAGCTCAAAATGGACCTCAACTCCACATATTTTAGAGAACAAGGTAATATACTTAGATTTTAATTTCCTTTTCCAAATATTTGATTTTTAATTTCTTTTTTCCTTTTTATATAACTCCATTATTCAATGTTTTTGCAGGCACAAATCTTCTGCTTTTCTCATTTCCCATCATGTTTATGGCAGTTTTGGGCTGTTTTTATCTTCATTTGCAGAAGAAATCTTCAGAAGATCCTAAACCCTTTTCTACCCAAAGGTTTTTGAACTTTGAATTTTTGTTTTTTTCTCTCAAATATTAAATTTTCGTTTTTTGAATGATTTTTTTTTTTTTTTTTTTGGGAATTGGGTTATTTTTGTAGGAGCCGAGGAGATTCATTTCTGAGACGACCAGTTCTGGTAATGAATTCTTTGGGGATTGTGAATACATTGGAGGCTATGTTCGCAGCTGCATTTGTTCTACTTTTGATTTGGTCTCTTGGAAATTACTTGTACGTCAGTTTTGGCCATCTTCACATGCATAAAGTTGGCGAGAAAATGTAAGTTTTCCTTCTTAATTAAGCTGTGATTTAGGCTTTATTTCATGCCTAAAAACACTTTTGGGATTCCCAAAAGAGTACTCGACTTTCGAATTCATCTTGTTTGGGAGTGATTTATAATTATATTTGAAAGAAGTCAAAAGCGCTCAAAAGAACAATACTTGTCTATTCACCGGTCAATAGAATACTTTACTGTCTTTCTCTCTAGTCTGATATATGTTCATTGGTCTAATTCTACTATGTTTAAGAGATAAGTAGACACTACAAAAGGTTTTGACCATTATATTAGGAGTTTGCACTTAAAATGCTTTATAGTTTGTGTTTGGTTCGAGTTTGTACAAATTTGTACTATGGCCTAGTTTGAGAGTGAATTTTAAAAGTTTAAGCTTATGTCGCCTATAAAATCATCAAAAAGTCCATGATACATATCAAATATAGGCTCAGACTCCATAGTTTCAAACTATATTACTCAACTTCTAAATCAAATGCATCAGTTAATTCTTCGAGTTTATTAATGATTTGAAACTCTTGTAGTAGCTAATTATTGTGTTTCGAGTTTGAATTTGTAACTTTTTTGGTCAGTTGGGAAGCAAAATTTCGAAGTGTATCATTGAGACTTGGGTACATTGGAAATATATGCTGGGCATTTCTGTTCTTTCCAGTCACAAGAGGCTCTTCTTTATTGCCTTTATTTGGACTCACATCAGAATCAACTATCAAATACCACATCTGGCTTGGCCACATTTCTAATTTTCTCTTTGCATTGCACACCGTTGGATTCATCACCTATTGGGCCATCACTGATCAAATGGCTTTGGTAAATTCATTTAATTACTTGTCTGATAAATCACTTTGATTTTGAAATAGAAAATGTGTGGTGTTCATCTTTTGGCTGGTCAGTTTGTCTTTTTGATTTTCTGATTGGTTTGTTTTCCTTGTTTTCTTTCAGTGCAAAGAATTATTCTTCCATTTTTTGATTCCTTATCCTTTTCTTGTCCTCTATATGCATTTGAAAGTGATTGATGCTTCATATGCATTTGAAAGTCATTGATCCTTTGAGATTTGTATTTAGACATATATCAGTCTTTCGCTTCAAAAATGTGTCATTACTATTTCCTTCGAATTCACTATTTATCGATATATAATTTATTCCGTAGTAAATTCAATTGTGCTGAAAACTAACTCGTTTGTATGAACAAAATAAGAAGAAAAATCATCTTTAGCTACAGCGACAAAATTCAACTCCAATGCTAGCTAGAACCAAGTTATAATTCTAGCTTCTTATGCGTTGTTCCTGAACTATGACTAATTGAGAATGTGCATCTCATTGAACAGTTGATAGAATGGAGTGTGGACTACGTATCAAATGTTGCTGGAGTGATTGCTATGGCATTTGCCACAGTTATCTGGGTGACGAGCTTTCGTCCAATTCGTCGAAAAATGTTTGAAGTCTTTTATTACAGTCACCATTTCTACATTCTCTACATCATCTTCTATCTAATCCATGTGGGAGCAGAATATGTATGCTTGATCCTTCCTGGAATCTTTCTCTTTGCTATCGATCGATACTTACGATTCTTACAGTCGCAACGTCAAGTAAGCCTAGTTTCAGCTCGACATCTTCCTTGTGGTGCTGTTGAACTAAACTTCTTGAAAAGCCCAGGTTTAGTTTCTCCAAGAGCTGATCATTGAAACCCTTCTTATTTTCTCTCTTTTGTCAAATATTTTATTTGAATTTGCCCTTTTCTGATCAGAACTAAGCTACAGTCCTACAAGTGTAATGTTCATAAATGTGCCAAGCATTTCCAAAATGCAGTGGCATCCCTTTACAGTCACTTCTAACTGCAAACTGGAGCCTGATATAGTGAGTGTTGTAATCAAAACTGGGGGAAGTTGGACTTCCAAACTTCACCAACAACTTTCTTCTTCTGTGGATCACCTTCAAGTTTCTGTTGAAGGTCCATATGGTCCTGCTTCTACTCACTTTTTAAGGTGATTCCCTATGAAGCCAAAATAGCCTTGAATTAGTGGAGTTTACTTAAACTTGATTGATGTTTTTTCTTTTGAAGGCATGAATCCCTGCTGCTGATAAGCGGAGGAAGCGGAATTACTCCCTTCATTTCGATAATCAGAGAGGTAATGATTGTTCGAAGCACGAAACCAAACTGCCATGTTCCGCAAATTCGCCTGATTTGTGCCTTCAAGAACTCCATCGATCTCAACATGCTGGATCTCTTGCTTCCAGTATCTAGCACACAAACAGAGATTTCAAATGTACCAATACAGATTGAAGCTTATATCACCAGAGAGAAACAGCAGGCTGAAACAAATGCTGAAAAGCTCATAAACACAAAATGGTTTAAGCCAAACCCCCTGGACTCACCAGTGTCAGAGGCTCTTGGACCAGACAATTGGCTGCTCCTTGGTGCAATTATCTCATCTTCATTTGTGATGTTCTTGATTCTTTTAGGCATCATCACTCGCTACTACGTTTACCCAATCGAACGAAATGGTGATGCCCTTTACAATTATAGCTATAAGGTTCTTTGGGACATGTTCTTGGCATTTGCTTGTATCTGCATATCTTCAAGCTTTGTCTTTCTGTGGGAAAGGAAAACAAATGCTTCAAAACACAAACAAATTCAAGTTCTCAATGTTCAATCACCAAAAGCATCACCTGGGTCATGGATCTACGGAGGAGAGAGAGAGCTCGAGAGCCTTCCCTGTCAATCACTTGTTCAAGCAACCAATATCCATTATGGAGGCAGGCCTGACCTCAACAGTAAGTTTTGCTGCCTTTCTATCAACCAAAAGCACCACATAAATATATAGCAACTAAAAATATCAATGATATATGACAATATGTTATGCAATGCAGAAATTTTGTTGGACTGCAAAGGATCGGATATTGGAGTTTTGGTCAGCGGACCGACTGGGATGCGGCATGACGTAGCAAAAATCTGTTCATCTGCTTATGCTGATAATCTAAATTTTCACTCCATGAGCTTCCTCTTTTAA

mRNA sequence

ATGGCAATGGCTAGAGCTCTAAAGATGATTTTCTTCTTAACATTTGTGCTTTGGCTGTGGCTTTGGATTATGTTGCCCACAAAGCTTTACAAGAACACTTGGACCCCTGAGCTCAAAATGGACCTCAACTCCACATATTTTAGAGAACAAGGCACAAATCTTCTGCTTTTCTCATTTCCCATCATGTTTATGGCAGTTTTGGGCTGTTTTTATCTTCATTTGCAGAAGAAATCTTCAGAAGATCCTAAACCCTTTTCTACCCAAAGGAGCCGAGGAGATTCATTTCTGAGACGACCAGTTCTGGTAATGAATTCTTTGGGGATTGTGAATACATTGGAGGCTATGTTCGCAGCTGCATTTGTTCTACTTTTGATTTGGTCTCTTGGAAATTACTTGTACGTCAGTTTTGGCCATCTTCACATGCATAAAGTTGGCGAGAAAATTTGGGAAGCAAAATTTCGAAGTGTATCATTGAGACTTGGGTACATTGGAAATATATGCTGGGCATTTCTGTTCTTTCCAGTCACAAGAGGCTCTTCTTTATTGCCTTTATTTGGACTCACATCAGAATCAACTATCAAATACCACATCTGGCTTGGCCACATTTCTAATTTTCTCTTTGCATTGCACACCGTTGGATTCATCACCTATTGGGCCATCACTGATCAAATGGCTTTGTTGATAGAATGGAGTGTGGACTACGTATCAAATGTTGCTGGAGTGATTGCTATGGCATTTGCCACAGTTATCTGGGTGACGAGCTTTCGTCCAATTCGTCGAAAAATGTTTGAAGTCTTTTATTACAGTCACCATTTCTACATTCTCTACATCATCTTCTATCTAATCCATGTGGGAGCAGAATATGTATGCTTGATCCTTCCTGGAATCTTTCTCTTTGCTATCGATCGATACTTACGATTCTTACAGTCGCAACGTCAAGTAAGCCTAGTTTCAGCTCGACATCTTCCTTGTGGTGCTGTTGAACTAAACTTCTTGAAAAGCCCAGAACTAAGCTACAGTCCTACAAGTGTAATGTTCATAAATGTGCCAAGCATTTCCAAAATGCAGTGGCATCCCTTTACAGTCACTTCTAACTGCAAACTGGAGCCTGATATAGTGAGTGTTGTAATCAAAACTGGGGGAAGTTGGACTTCCAAACTTCACCAACAACTTTCTTCTTCTGTGGATCACCTTCAAGTTTCTGTTGAAGGTCCATATGGTCCTGCTTCTACTCACTTTTTAAGGCATGAATCCCTGCTGCTGATAAGCGGAGGAAGCGGAATTACTCCCTTCATTTCGATAATCAGAGAGGTAATGATTGTTCGAAGCACGAAACCAAACTGCCATGTTCCGCAAATTCGCCTGATTTGTGCCTTCAAGAACTCCATCGATCTCAACATGCTGGATCTCTTGCTTCCAGTATCTAGCACACAAACAGAGATTTCAAATGTACCAATACAGATTGAAGCTTATATCACCAGAGAGAAACAGCAGGCTGAAACAAATGCTGAAAAGCTCATAAACACAAAATGGTTTAAGCCAAACCCCCTGGACTCACCAGTGTCAGAGGCTCTTGGACCAGACAATTGGCTGCTCCTTGGTGCAATTATCTCATCTTCATTTGTGATGTTCTTGATTCTTTTAGGCATCATCACTCGCTACTACGTTTACCCAATCGAACGAAATGGTGATGCCCTTTACAATTATAGCTATAAGGTTCTTTGGGACATGTTCTTGGCATTTGCTTGTATCTGCATATCTTCAAGCTTTGTCTTTCTGTGGGAAAGGAAAACAAATGCTTCAAAACACAAACAAATTCAAGTTCTCAATGTTCAATCACCAAAAGCATCACCTGGGTCATGGATCTACGGAGGAGAGAGAGAGCTCGAGAGCCTTCCCTGTCAATCACTTGTTCAAGCAACCAATATCCATTATGGAGGCAGGCCTGACCTCAACAAAATTTTGTTGGACTGCAAAGGATCGGATATTGGAGTTTTGGTCAGCGGACCGACTGGGATGCGGCATGACGTAGCAAAAATCTGTTCATCTGCTTATGCTGATAATCTAAATTTTCACTCCATGAGCTTCCTCTTTTAA

Coding sequence (CDS)

ATGGCAATGGCTAGAGCTCTAAAGATGATTTTCTTCTTAACATTTGTGCTTTGGCTGTGGCTTTGGATTATGTTGCCCACAAAGCTTTACAAGAACACTTGGACCCCTGAGCTCAAAATGGACCTCAACTCCACATATTTTAGAGAACAAGGCACAAATCTTCTGCTTTTCTCATTTCCCATCATGTTTATGGCAGTTTTGGGCTGTTTTTATCTTCATTTGCAGAAGAAATCTTCAGAAGATCCTAAACCCTTTTCTACCCAAAGGAGCCGAGGAGATTCATTTCTGAGACGACCAGTTCTGGTAATGAATTCTTTGGGGATTGTGAATACATTGGAGGCTATGTTCGCAGCTGCATTTGTTCTACTTTTGATTTGGTCTCTTGGAAATTACTTGTACGTCAGTTTTGGCCATCTTCACATGCATAAAGTTGGCGAGAAAATTTGGGAAGCAAAATTTCGAAGTGTATCATTGAGACTTGGGTACATTGGAAATATATGCTGGGCATTTCTGTTCTTTCCAGTCACAAGAGGCTCTTCTTTATTGCCTTTATTTGGACTCACATCAGAATCAACTATCAAATACCACATCTGGCTTGGCCACATTTCTAATTTTCTCTTTGCATTGCACACCGTTGGATTCATCACCTATTGGGCCATCACTGATCAAATGGCTTTGTTGATAGAATGGAGTGTGGACTACGTATCAAATGTTGCTGGAGTGATTGCTATGGCATTTGCCACAGTTATCTGGGTGACGAGCTTTCGTCCAATTCGTCGAAAAATGTTTGAAGTCTTTTATTACAGTCACCATTTCTACATTCTCTACATCATCTTCTATCTAATCCATGTGGGAGCAGAATATGTATGCTTGATCCTTCCTGGAATCTTTCTCTTTGCTATCGATCGATACTTACGATTCTTACAGTCGCAACGTCAAGTAAGCCTAGTTTCAGCTCGACATCTTCCTTGTGGTGCTGTTGAACTAAACTTCTTGAAAAGCCCAGAACTAAGCTACAGTCCTACAAGTGTAATGTTCATAAATGTGCCAAGCATTTCCAAAATGCAGTGGCATCCCTTTACAGTCACTTCTAACTGCAAACTGGAGCCTGATATAGTGAGTGTTGTAATCAAAACTGGGGGAAGTTGGACTTCCAAACTTCACCAACAACTTTCTTCTTCTGTGGATCACCTTCAAGTTTCTGTTGAAGGTCCATATGGTCCTGCTTCTACTCACTTTTTAAGGCATGAATCCCTGCTGCTGATAAGCGGAGGAAGCGGAATTACTCCCTTCATTTCGATAATCAGAGAGGTAATGATTGTTCGAAGCACGAAACCAAACTGCCATGTTCCGCAAATTCGCCTGATTTGTGCCTTCAAGAACTCCATCGATCTCAACATGCTGGATCTCTTGCTTCCAGTATCTAGCACACAAACAGAGATTTCAAATGTACCAATACAGATTGAAGCTTATATCACCAGAGAGAAACAGCAGGCTGAAACAAATGCTGAAAAGCTCATAAACACAAAATGGTTTAAGCCAAACCCCCTGGACTCACCAGTGTCAGAGGCTCTTGGACCAGACAATTGGCTGCTCCTTGGTGCAATTATCTCATCTTCATTTGTGATGTTCTTGATTCTTTTAGGCATCATCACTCGCTACTACGTTTACCCAATCGAACGAAATGGTGATGCCCTTTACAATTATAGCTATAAGGTTCTTTGGGACATGTTCTTGGCATTTGCTTGTATCTGCATATCTTCAAGCTTTGTCTTTCTGTGGGAAAGGAAAACAAATGCTTCAAAACACAAACAAATTCAAGTTCTCAATGTTCAATCACCAAAAGCATCACCTGGGTCATGGATCTACGGAGGAGAGAGAGAGCTCGAGAGCCTTCCCTGTCAATCACTTGTTCAAGCAACCAATATCCATTATGGAGGCAGGCCTGACCTCAACAAAATTTTGTTGGACTGCAAAGGATCGGATATTGGAGTTTTGGTCAGCGGACCGACTGGGATGCGGCATGACGTAGCAAAAATCTGTTCATCTGCTTATGCTGATAATCTAAATTTTCACTCCATGAGCTTCCTCTTTTAA

Protein sequence

MAMARALKMIFFLTFVLWLWLWIMLPTKLYKNTWTPELKMDLNSTYFREQGTNLLLFSFPIMFMAVLGCFYLHLQKKSSEDPKPFSTQRSRGDSFLRRPVLVMNSLGIVNTLEAMFAAAFVLLLIWSLGNYLYVSFGHLHMHKVGEKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRGSSLLPLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQMALLIEWSVDYVSNVAGVIAMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFLFAIDRYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWHPFTVTSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSSVDHLQVSVEGPYGPASTHFLRHESLLLISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPVSSTQTEISNVPIQIEAYITREKQQAETNAEKLINTKWFKPNPLDSPVSEALGPDNWLLLGAIISSSFVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSFVFLWERKTNASKHKQIQVLNVQSPKASPGSWIYGGERELESLPCQSLVQATNIHYGGRPDLNKILLDCKGSDIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSFLF
Homology
BLAST of Lag0020043 vs. NCBI nr
Match: XP_023552403.1 (ferric reduction oxidase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1247.6 bits (3227), Expect = 0.0e+00
Identity = 608/696 (87.36%), Postives = 656/696 (94.25%), Query Frame = 0

Query: 1   MAMARALKMIFFLTFVLWLWLWIMLPTKLYKNTWTPELKMDLNSTYFREQGTNLLLFSFP 60
           MAM R +KM+F LTFVLWLWLWIMLPTKLYKNTWTP+L MDLNSTYFREQGTNLLLFSFP
Sbjct: 5   MAMVRGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLLFSFP 64

Query: 61  IMFMAVLGCFYLHLQKKSSEDPKPFSTQRSRGDSFLRRPVLVMNSLGIVNTLEAMFAAAF 120
           +M MAVLGCFYLHLQK SSEDPKPFSTQRSRGDSFL+RP+LVMN LGI+NTLE  FAAAF
Sbjct: 65  VMLMAVLGCFYLHLQKSSSEDPKPFSTQRSRGDSFLKRPILVMNPLGILNTLEIGFAAAF 124

Query: 121 VLLLIWSLGNYLYVSFGHLHMHKVGEKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRGSS 180
           V+LLIWSLGNYLYVSFG+LHMH  GEK+WEAKFRSVSLRLGYIG+ICWAFLFFPVTRGSS
Sbjct: 125 VILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVTRGSS 184

Query: 181 LLPLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQMALLIEWSVDYVSNVAG 240
           LLP+ GLTSESTIKYHIWLGHISN LFALHTVGFI YW ITDQMALLIEWS DYVSN+AG
Sbjct: 185 LLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVSNIAG 244

Query: 241 VIAMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFLFA 300
           +IAMAFATVIWVTSFR IRRKMFEVF+YSHHFY LY +FYLIHVGA YVCL+LPG+FLFA
Sbjct: 245 LIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLVLPGMFLFA 304

Query: 301 IDRYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWHPF 360
           IDR++RFLQSQR+VSLVSARHLPCGAVELN LKSPELSYSPTSVMF+NVPSISK+QWHPF
Sbjct: 305 IDRFIRFLQSQRRVSLVSARHLPCGAVELNLLKSPELSYSPTSVMFLNVPSISKLQWHPF 364

Query: 361 TVTSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSSVDHLQVSVEGPYGPASTHFLRHESLL 420
           TVTSN KLEPDI+SVV K GGSWTSKLHQQLSSS+DHLQVSVEGPYGPASTHFLRHESLL
Sbjct: 365 TVTSNSKLEPDIISVVSKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTHFLRHESLL 424

Query: 421 LISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPVSSTQTE 480
           LISGGSGITPFISIIRE+MIVRSTKPNCHVPQ+RL+CAFKNSIDL MLDLLLPVSSTQTE
Sbjct: 425 LISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSSTQTE 484

Query: 481 ISNVPIQIEAYITREKQQAETNAEKLINTKWFKPNPLDSPVSEALGPDNWLLLGAIISSS 540
           ISNVP+QI+AYITRE++Q +TN E LINT WFKPNPLDSPV+EALGP+NWLLLGA+ISSS
Sbjct: 485 ISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPLDSPVTEALGPNNWLLLGAVISSS 544

Query: 541 FVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSFVFLWERKTNA 600
           FVMFLILLGIITRYY+YPIERNGDA+YNY+YKVLWDMFL+ ACICI SS V +W+RKT  
Sbjct: 545 FVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQRKTYV 604

Query: 601 SKHKQIQVLNVQSPKASPGSWIYGGERELESLPCQSLVQATNIHYGGRPDLNKILLDCKG 660
           S +KQ+QVLNVQSPKASPGSWI+GGERELESLP Q LVQATNIHYGGRPDL +ILLDCKG
Sbjct: 605 S-NKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLERILLDCKG 664

Query: 661 SDIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
           SDIGVLVSGPTGMRHDVAKICS+AY DNLNFH+MSF
Sbjct: 665 SDIGVLVSGPTGMRHDVAKICSTAYGDNLNFHAMSF 699

BLAST of Lag0020043 vs. NCBI nr
Match: XP_038878180.1 (ferric reduction oxidase 4-like [Benincasa hispida])

HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 621/701 (88.59%), Postives = 657/701 (93.72%), Query Frame = 0

Query: 3   MARALKMIFFLTFVLWLWLWIMLPTKLYKNTWTPELKMDLNSTYFREQGTNLLLFSFPIM 62
           MA+ALKMIF L FVLWLWLWIMLPTKLYKN WTP+L M+LNSTYFREQGTNLLLFSFPIM
Sbjct: 5   MAKALKMIFLLIFVLWLWLWIMLPTKLYKNNWTPKLNMNLNSTYFREQGTNLLLFSFPIM 64

Query: 63  FMAVLGCFYLHLQKKSSE-DPKPFSTQRSRGDSFLRRPVLVMNSLGIVNTLEAMFAAAFV 122
            MAVLGCFYLHLQKKSSE DPKPFSTQRSRGDSFL+RP++VMN  GIVNTLE +FAAAF+
Sbjct: 65  LMAVLGCFYLHLQKKSSELDPKPFSTQRSRGDSFLKRPIVVMNPFGIVNTLEIVFAAAFL 124

Query: 123 LLLIWSLGNYLYVSFGHLHMHKVGEKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRGSSL 182
           LLLIWSLGNYLYVSFG+LHM  VGE I EAKFRSVSLR+GYIGNICWAFLFFPVTRGSSL
Sbjct: 125 LLLIWSLGNYLYVSFGNLHMDNVGETIGEAKFRSVSLRVGYIGNICWAFLFFPVTRGSSL 184

Query: 183 LPLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQMALLIEWSVDYVSNVAGV 242
           LPL GLTSESTIKYHIWLGHISN LFALHT+GFI YWAITDQMALL EWS DYVSNVAGV
Sbjct: 185 LPLIGLTSESTIKYHIWLGHISNLLFALHTLGFIIYWAITDQMALLKEWSEDYVSNVAGV 244

Query: 243 IAMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFLFAI 302
           IAMAFATVIWVTSF+ IRRKMFEVFYYSHHFY LYIIFYLIHVGA YVCLILPGIFL+AI
Sbjct: 245 IAMAFATVIWVTSFKSIRRKMFEVFYYSHHFYALYIIFYLIHVGAAYVCLILPGIFLWAI 304

Query: 303 DRYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWHPFT 362
           DR+LRFLQSQR VSLVSARHLPCGAVELNFLKSPELSY PTSVMFINVPSISK+QWHPFT
Sbjct: 305 DRFLRFLQSQRHVSLVSARHLPCGAVELNFLKSPELSYKPTSVMFINVPSISKLQWHPFT 364

Query: 363 VTSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSSVDHLQVSVEGPYGPASTHFLRHESLLL 422
           VTSNCKLEPD VSVVIK+GGSWTSKLH+QLSSS+DHLQVSVEGPYGPASTHFLRHESLLL
Sbjct: 365 VTSNCKLEPDTVSVVIKSGGSWTSKLHKQLSSSLDHLQVSVEGPYGPASTHFLRHESLLL 424

Query: 423 ISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPVSSTQTEI 482
           ISGGSGITPFISIIRE+MIVRSTKPNCHVPQIRL+CAFKNSIDL MLDLLLPVSSTQTEI
Sbjct: 425 ISGGSGITPFISIIREIMIVRSTKPNCHVPQIRLVCAFKNSIDLTMLDLLLPVSSTQTEI 484

Query: 483 SNVPIQIEAYITREKQQAETNAEKLINTKWFKPNPLDSPVSEALGPDNWLLLGAIISSSF 542
           SN+P+QIEAYITREK+Q +TN+E LINT WFKPNPLDSPVSEALGP+NWLLLGAIISSSF
Sbjct: 485 SNIPLQIEAYITREKEQPQTNSENLINTIWFKPNPLDSPVSEALGPNNWLLLGAIISSSF 544

Query: 543 VMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSFVFLWERKTNAS 602
           VMFLILLGIITRYY+YPIE+NGDA+YNY+YKVLWDMFLAFACICISSSFVFLW+RKTNAS
Sbjct: 545 VMFLILLGIITRYYIYPIEQNGDAVYNYTYKVLWDMFLAFACICISSSFVFLWQRKTNAS 604

Query: 603 KHKQIQVLNVQSPKASPGSWIYGGERELESLPCQSLVQATNIHYGGRPDLN------KIL 662
             KQIQ+ N QSPKASPGSWI+ G+ ELESLPCQSLVQAT+IHYGGRPDL       KIL
Sbjct: 605 NGKQIQIHNFQSPKASPGSWIFRGDSELESLPCQSLVQATSIHYGGRPDLKKILFDCKIL 664

Query: 663 LDCKGSDIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
            DCKGSDIGVLVSGPTGMRHDVAKICS+AY DNL FHSMSF
Sbjct: 665 FDCKGSDIGVLVSGPTGMRHDVAKICSTAYGDNLKFHSMSF 705

BLAST of Lag0020043 vs. NCBI nr
Match: XP_022922912.1 (ferric reduction oxidase 4-like [Cucurbita moschata])

HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 609/696 (87.50%), Postives = 656/696 (94.25%), Query Frame = 0

Query: 1   MAMARALKMIFFLTFVLWLWLWIMLPTKLYKNTWTPELKMDLNSTYFREQGTNLLLFSFP 60
           MAMAR +KM+F LTFVLWLWLWIMLPTKLYKNTWTP+L MDLNSTYFREQGTNLLLFSFP
Sbjct: 5   MAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLLFSFP 64

Query: 61  IMFMAVLGCFYLHLQKKSSEDPKPFSTQRSRGDSFLRRPVLVMNSLGIVNTLEAMFAAAF 120
           +M MAVLGCFYLHLQK SSEDPKPFSTQRSRGDSFL+RPVLVMN LGI+NTLE  FAAAF
Sbjct: 65  VMLMAVLGCFYLHLQKSSSEDPKPFSTQRSRGDSFLKRPVLVMNPLGILNTLEIGFAAAF 124

Query: 121 VLLLIWSLGNYLYVSFGHLHMHKVGEKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRGSS 180
           V+LLIWSLGNYLYVSFG+LHMH  GEK+WEAKFRSVSLRLGYIG+ICWAFLFFPVTRGSS
Sbjct: 125 VILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVTRGSS 184

Query: 181 LLPLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQMALLIEWSVDYVSNVAG 240
           LLP+ GLTSESTIKYHIWLGHISN LFALHTVGFI YW ITDQMALLIEWS DYVSN+AG
Sbjct: 185 LLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVSNIAG 244

Query: 241 VIAMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFLFA 300
           +IAMAFATVIWVTSFR IRRKMFEVF+YSHHFY LY +FYLIHVGA YVCL+LPG+FLFA
Sbjct: 245 LIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLVLPGMFLFA 304

Query: 301 IDRYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWHPF 360
           IDR+LRFLQSQR+VSLVSARHLPCGAVELN LKSPELSYSPTSVMF+NVP+ISK+QWHPF
Sbjct: 305 IDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSPELSYSPTSVMFLNVPTISKLQWHPF 364

Query: 361 TVTSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSSVDHLQVSVEGPYGPASTHFLRHESLL 420
           TVTSN KLEP I+SV IK GGSWTSKLHQQLSSS+DHLQVSVEGPYGPASTHFLRHESLL
Sbjct: 365 TVTSNSKLEPAIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTHFLRHESLL 424

Query: 421 LISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPVSSTQTE 480
           LISGGSGITPFISIIRE+MIVRSTKPNCHVPQ+RL+CAFKNSIDL MLDLLLPVSSTQTE
Sbjct: 425 LISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSSTQTE 484

Query: 481 ISNVPIQIEAYITREKQQAETNAEKLINTKWFKPNPLDSPVSEALGPDNWLLLGAIISSS 540
           ISNVP+QI+AYITRE++Q +TN E LINT WFKPNPLDSPV+EALGP+NWLLLGA+ISSS
Sbjct: 485 ISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPLDSPVTEALGPNNWLLLGAVISSS 544

Query: 541 FVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSFVFLWERKTNA 600
           FVMFLILLGIITRYY+YPIERNGDA+YNY+YKVLWDMFL+ ACICI SS V +W+RKT  
Sbjct: 545 FVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQRKTYV 604

Query: 601 SKHKQIQVLNVQSPKASPGSWIYGGERELESLPCQSLVQATNIHYGGRPDLNKILLDCKG 660
           S +KQ+QVLNVQSPKASPGSWI+GGERELESLP Q LVQATNIHYGGRPDL +ILLDCKG
Sbjct: 605 S-NKQVQVLNVQSPKASPGSWIFGGERELESLPFQPLVQATNIHYGGRPDLKRILLDCKG 664

Query: 661 SDIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
           SDIGVLVSGPTGMRHDVAKICS+AY DNLNFH+MSF
Sbjct: 665 SDIGVLVSGPTGMRHDVAKICSTAYGDNLNFHAMSF 699

BLAST of Lag0020043 vs. NCBI nr
Match: KAG6576705.1 (Ferric reduction oxidase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1245.3 bits (3221), Expect = 0.0e+00
Identity = 608/696 (87.36%), Postives = 656/696 (94.25%), Query Frame = 0

Query: 1   MAMARALKMIFFLTFVLWLWLWIMLPTKLYKNTWTPELKMDLNSTYFREQGTNLLLFSFP 60
           MAMAR +KM+F LTFVLWLWLWIMLPTKLYKNTWTP+L MDLNSTYFREQGTNLLLFSFP
Sbjct: 5   MAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLLFSFP 64

Query: 61  IMFMAVLGCFYLHLQKKSSEDPKPFSTQRSRGDSFLRRPVLVMNSLGIVNTLEAMFAAAF 120
           +M MAVLGCFYLHLQK SSEDPKPFSTQRSRGDSFL+RPVLVMN LGI+NTLE  FAAAF
Sbjct: 65  VMLMAVLGCFYLHLQKSSSEDPKPFSTQRSRGDSFLKRPVLVMNPLGILNTLEIGFAAAF 124

Query: 121 VLLLIWSLGNYLYVSFGHLHMHKVGEKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRGSS 180
           V+LLIWSLGNYLYVSFG+LHMH  GEK+WEAKFRSVSLRLGYIG+ICWAFLFFPVTRGSS
Sbjct: 125 VILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVTRGSS 184

Query: 181 LLPLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQMALLIEWSVDYVSNVAG 240
           LLP+ GLTSESTIKYHIWLGHISN LFALHTVGFI YW ITDQMALLIEWS DYVSN+AG
Sbjct: 185 LLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVSNIAG 244

Query: 241 VIAMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFLFA 300
           +IAMAFATVIWVTSFR IRRKMFEVF+YSHHFY LY +FYLIHVGA YVCL+LPG+FLFA
Sbjct: 245 LIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLVLPGMFLFA 304

Query: 301 IDRYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWHPF 360
           IDR+LRFLQSQR+VSLVSARHLPCGAVELN LKSPELSYSPTSVMF+NV +ISK+QWHPF
Sbjct: 305 IDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSPELSYSPTSVMFLNVTTISKLQWHPF 364

Query: 361 TVTSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSSVDHLQVSVEGPYGPASTHFLRHESLL 420
           TVTSN KLEPDI+SV IK GGSWTSKLHQQLSSS+DHLQVSVEGPYGPASTHFLRHESLL
Sbjct: 365 TVTSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTHFLRHESLL 424

Query: 421 LISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPVSSTQTE 480
           LISGGSGITPFISIIRE+MIVRSTKPNCHVPQ+RL+CAFKNSIDL MLDLLLPVSSTQTE
Sbjct: 425 LISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSSTQTE 484

Query: 481 ISNVPIQIEAYITREKQQAETNAEKLINTKWFKPNPLDSPVSEALGPDNWLLLGAIISSS 540
           ISNVP+QI+AYITRE++Q +TN E LINT WFKPNPLDSPV+EALGP+NWLLLGA+ISSS
Sbjct: 485 ISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPLDSPVTEALGPNNWLLLGAVISSS 544

Query: 541 FVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSFVFLWERKTNA 600
           FVMFLILLGIITRYY+YPIERNGDA+YNY+YKVLWDMFL+ ACICI SS V +W+RKT  
Sbjct: 545 FVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQRKTYV 604

Query: 601 SKHKQIQVLNVQSPKASPGSWIYGGERELESLPCQSLVQATNIHYGGRPDLNKILLDCKG 660
           S +KQ+QVLNVQSPKASPGSWI+GGERELESLP Q LVQATNIHYGGRPDL +ILLDCKG
Sbjct: 605 S-NKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLDCKG 664

Query: 661 SDIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
           SDIGVLVSGPTGMRHDVAKICS+A+ DNLNFH+MSF
Sbjct: 665 SDIGVLVSGPTGMRHDVAKICSTAFGDNLNFHAMSF 699

BLAST of Lag0020043 vs. NCBI nr
Match: XP_022984974.1 (ferric reduction oxidase 4-like [Cucurbita maxima])

HSP 1 Score: 1235.7 bits (3196), Expect = 0.0e+00
Identity = 604/696 (86.78%), Postives = 651/696 (93.53%), Query Frame = 0

Query: 1   MAMARALKMIFFLTFVLWLWLWIMLPTKLYKNTWTPELKMDLNSTYFREQGTNLLLFSFP 60
           MAMAR +KM+F LTFVLWLWLWIMLPTKLYKNTWTP+L MDLNSTYFREQGTNLLLFSFP
Sbjct: 5   MAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLLFSFP 64

Query: 61  IMFMAVLGCFYLHLQKKSSEDPKPFSTQRSRGDSFLRRPVLVMNSLGIVNTLEAMFAAAF 120
           +M MAVLGCFYL LQK SSEDPKPFSTQRSRGDSFL+RPVL MN LGI+NTLE  FAAAF
Sbjct: 65  VMLMAVLGCFYLQLQKSSSEDPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIGFAAAF 124

Query: 121 VLLLIWSLGNYLYVSFGHLHMHKVGEKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRGSS 180
           V+LLIWSLGNYLYVSFG+LHMH  GEK+WEAKFRSVSLRLGYIG+ICWAFLFFPVTRGSS
Sbjct: 125 VILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVTRGSS 184

Query: 181 LLPLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQMALLIEWSVDYVSNVAG 240
           LLP+ GLTSESTIKYHIWLGHISN LFALHTVGFI YW ITDQMALLIEWS DYVSN+AG
Sbjct: 185 LLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVSNIAG 244

Query: 241 VIAMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFLFA 300
           +IAMAFATVIWVTSFR IRRKMFEVF+YSHHFY LY +FYLIHVGA YVCLILPG+FLFA
Sbjct: 245 LIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGMFLFA 304

Query: 301 IDRYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWHPF 360
           IDR+LRFLQSQR+VSLVSARHLPCGAVELN LKS ELSYSPTSVMF+NVP+ISK+QWHPF
Sbjct: 305 IDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQWHPF 364

Query: 361 TVTSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSSVDHLQVSVEGPYGPASTHFLRHESLL 420
           T+TSN KLEPDI+SV IK GGSWTSKLHQQLSSS+DHLQVSVEGPYGPAST FLRHESLL
Sbjct: 365 TITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLRHESLL 424

Query: 421 LISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPVSSTQTE 480
           LISGGSGITPFISIIRE+MIVRSTKPNCHVPQ+RL+CAFKNSIDL MLDLLLPVSSTQTE
Sbjct: 425 LISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSSTQTE 484

Query: 481 ISNVPIQIEAYITREKQQAETNAEKLINTKWFKPNPLDSPVSEALGPDNWLLLGAIISSS 540
           ISNVP+QI+AYITRE++Q +TN E LINT WFKPNP+DSPVSEALGP+NWLLLG +ISSS
Sbjct: 485 ISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLLGTVISSS 544

Query: 541 FVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSFVFLWERKTNA 600
           FVMFLILLGIITRYY+YPIERNGDA+YNY+YKVLWDMFL+ ACICI SS V +W+RKT  
Sbjct: 545 FVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQRKTYV 604

Query: 601 SKHKQIQVLNVQSPKASPGSWIYGGERELESLPCQSLVQATNIHYGGRPDLNKILLDCKG 660
           S +KQ+QVLNVQSPKASPGSWI+GGERELESLP Q LVQATNIHYGGRPDL +ILLDCKG
Sbjct: 605 S-NKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLDCKG 664

Query: 661 SDIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
           SDIGVLVSGP GMRHDVAKICS+AY DNLNFH+MSF
Sbjct: 665 SDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSF 699

BLAST of Lag0020043 vs. ExPASy Swiss-Prot
Match: Q8W110 (Ferric reduction oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=FRO4 PE=2 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 1.1e-226
Identity = 391/701 (55.78%), Postives = 519/701 (74.04%), Query Frame = 0

Query: 3   MARALKMIFFLTFVLWLWLWIMLPTKLYKNTWTPELKMDLNSTYFREQGTNLLLFSFPIM 62
           M   +K +  + F+ W+ +WIM+ T L+K+ WTP+L   LN+TYF  QGTNL+L + P+M
Sbjct: 4   MRSLVKTLMVVLFLGWILVWIMISTNLFKSKWTPKLSKYLNTTYFGPQGTNLVLLTVPMM 63

Query: 63  FMAVLGCFYLHLQKKSSEDPKPFSTQRSRGDSFLRRPVLVMNSLGIVNTLEAMFAAAFVL 122
           F+AVL C YLH+QKK ++  + +  +R  G     R ++VMN LGIV   E  F+  FV 
Sbjct: 64  FIAVLSCVYLHIQKKPTQPQREWKLKRIMG-----RVIMVMNPLGIVTATELTFSLLFVA 123

Query: 123 LLIWSLGNYLYVSFGHLHMHK-VGEKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRGSSL 182
           LL WSL NYLY+S+ H+H+H     KIW+AKFR+  LR+GY+GNICWAFLFFPVTR S++
Sbjct: 124 LLAWSLYNYLYLSY-HVHLHNDDNAKIWQAKFRAFGLRIGYVGNICWAFLFFPVTRASTI 183

Query: 183 LPLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQMALLIEWSVDYVSNVAGV 242
           LPL GLTSES+IKYHIWLGH+SNF F +HTV F+ YWA+ +++     W+  YV N+AG 
Sbjct: 184 LPLVGLTSESSIKYHIWLGHVSNFCFLVHTVVFLIYWAMINKLMETFAWNPTYVPNLAGT 243

Query: 243 IAMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFLFAI 302
           IAM     +WVTS    RRK FE+F+Y+HH Y LYI+FY+IHVG  + C+ILP IFLF I
Sbjct: 244 IAMVIGIAMWVTSLPSFRRKKFEIFFYTHHLYGLYIVFYVIHVGDSWFCMILPNIFLFFI 303

Query: 303 DRYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWHPFT 362
           DRYLRFLQS ++  LVSAR LP   +EL F K+P L Y+PTS++F++VPSISK+QWHPFT
Sbjct: 304 DRYLRFLQSTKRSRLVSARILPSDNLELTFSKTPGLHYTPTSILFLHVPSISKIQWHPFT 363

Query: 363 VTSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSSVDHLQVSVEGPYGPASTHFLRHESLLL 422
           +TS+  LE D +SVVI+  GSWT KL+  LSSS+D L+VS EGPYGP S    RH SL+L
Sbjct: 364 ITSSSNLEKDTLSVVIRRQGSWTQKLYTHLSSSIDSLEVSTEGPYGPNSFDVSRHNSLIL 423

Query: 423 ISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPVSSTQTEI 482
           +SGGSGITPFIS+IRE +I +S   +  +P + L+C+FK+  DL  LDL+ P+  + ++I
Sbjct: 424 VSGGSGITPFISVIRE-LISQSQNKSTKLPDVLLVCSFKHYHDLAFLDLIFPLDMSASDI 483

Query: 483 SNVPIQIEAYITREKQQAETNAE-KLINTKWFKPNPLDSPVSEALGPDNWLLLGAIISSS 542
           S + ++IEAYITRE ++ ET  + +L+ TKWFKP PLDSP+S  LGP+N+L LG +I SS
Sbjct: 484 SRLNLRIEAYITREDKKPETTDDHRLLQTKWFKPQPLDSPISPVLGPNNFLWLGVVILSS 543

Query: 543 FVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSFVFLWERKTNA 602
           FVMFL+L+GI+TRYY+YP++ N  ++YN+SY+ LWDMFL  ACI ISSS VFLW +K N 
Sbjct: 544 FVMFLLLIGIVTRYYIYPVDHNTGSIYNFSYRGLWDMFLGSACIFISSSVVFLWRKKQNK 603

Query: 603 SKHK----QIQVLNVQSPKASPGSWIYGGERELESLPCQSLVQATNIHYGGRPDLNKILL 662
              K    Q+Q +  Q+P +SPGSW +G ERELES+P QS+VQAT++H+G +P+L KILL
Sbjct: 604 EGDKEFKNQVQSVEFQTPTSSPGSWFHGHERELESVPYQSIVQATSVHFGSKPNLKKILL 663

Query: 663 DCKGS-DIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
           + +GS D+GV+V GP  MRH+VAKICSS  A NL+F ++SF
Sbjct: 664 EAEGSEDVGVMVCGPRKMRHEVAKICSSGLAKNLHFEAISF 697

BLAST of Lag0020043 vs. ExPASy Swiss-Prot
Match: Q9FLW2 (Ferric reduction oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=FRO5 PE=2 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 8.7e-221
Identity = 378/703 (53.77%), Postives = 509/703 (72.40%), Query Frame = 0

Query: 3   MARALKMIFFLTFVLWLWLWIMLPTKLYKNTWTPELKMDLNSTYFREQGTNLLLFSFPIM 62
           M   +KM+  + F+ W+++WIM+ T  ++N WTP+L   L +TYF  QG NL+L + P+M
Sbjct: 4   MRSLVKMLMVVLFLGWIFVWIMISTNRFQNIWTPKLAKYLKTTYFGPQGMNLVLLTVPMM 63

Query: 63  FMAVLGCFYLHLQKKSSEDPKPFSTQRSRGDSFLRRPVLVMNSLGIVNTLEAMFAAAFVL 122
           F+AVL C YLH QK+ S+    +  +  +    + R ++VMN LGIV   E  F+  F+ 
Sbjct: 64  FIAVLSCVYLHTQKQPSQTQSLYKCREWKVKGRMGRVMMVMNPLGIVTATELTFSLLFLA 123

Query: 123 LLIWSLGNYLYVSFG-HLHMHKVGEKI--WEAKFRSVSLRLGYIGNICWAFLFFPVTRGS 182
           LL+W+L NYLY+S+  HLH H     +  W+AKFR+  LR+GY+G+ CWAFLFFPVTR S
Sbjct: 124 LLVWALSNYLYLSYHVHLHNHDNANDMCRWQAKFRAFGLRIGYVGHYCWAFLFFPVTRAS 183

Query: 183 SLLPLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQMALLIEWSVDYVSNVA 242
           ++LPL GLTSES+IKYHIWLGH+SNF F +HTV F+ YWA+ +++     W+  YV N+A
Sbjct: 184 TILPLVGLTSESSIKYHIWLGHVSNFCFLVHTVVFLIYWAMVNKLMETFAWNATYVPNLA 243

Query: 243 GVIAMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFLF 302
           G IAM     IWVTS    RRK FE+F+Y+HH Y LYI+FY IHVG  + C+ILP IFLF
Sbjct: 244 GTIAMVIGIAIWVTSLPSFRRKKFEIFFYTHHLYGLYIVFYAIHVGDSWFCMILPNIFLF 303

Query: 303 AIDRYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWHP 362
            IDRYLRFLQS ++  LVSA+ LP   +EL F K+  L Y+PTS++F++VPSISK+QWHP
Sbjct: 304 FIDRYLRFLQSTKRSRLVSAKILPSDNLELTFAKTSGLHYTPTSILFLHVPSISKLQWHP 363

Query: 363 FTVTSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSSVDHLQVSVEGPYGPASTHFLRHESL 422
           FT+TS+  LE D +SVVI+  GSWT KL+  LSSS+D L+VS EGPYGP S    RH+SL
Sbjct: 364 FTITSSSNLEKDTLSVVIRKQGSWTQKLYTHLSSSIDSLEVSTEGPYGPNSFDVSRHDSL 423

Query: 423 LLISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPVSSTQT 482
           +L+ GGSG+TPFIS+IRE +I +S   +  +P + L+CAFKN  DL  LDL+ P   + +
Sbjct: 424 ILVGGGSGVTPFISVIRE-LIFQSQNRSTKLPNVLLVCAFKNYHDLAFLDLIFPSDISVS 483

Query: 483 EISNVPIQIEAYITREKQQAETNAE-KLINTKWFKPNPLDSPVSEALGPDNWLLLGAIIS 542
           +IS + ++IEAYITRE ++ ET  + KL+ TKWFKP PLDSP+S  LGP+N+L LG +I 
Sbjct: 484 DISKLNLRIEAYITREDKKPETTDDHKLLQTKWFKPQPLDSPISPVLGPNNFLWLGVVIL 543

Query: 543 SSFVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSFVFLWERKT 602
           SSFVMFL+L+GI+TRYY+YP++ N  ++YN++Y+VLW MFL   CI ISSS +FLW +K 
Sbjct: 544 SSFVMFLLLIGIVTRYYIYPVDHNTGSIYNFTYRVLWVMFLGCVCIFISSSIIFLWRKKE 603

Query: 603 N----ASKHKQIQVLNVQSPKASPGSWIYGGERELESLPCQSLVQATNIHYGGRPDLNKI 662
           N        KQ+Q +  Q+P +SPGSW +G ERELES+P QS+VQAT++H+G +P+L KI
Sbjct: 604 NKEGDKDSKKQVQSVEFQTPTSSPGSWFHGHERELESVPYQSIVQATSVHFGSKPNLKKI 663

Query: 663 LLDCKGS-DIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
           L + +GS D+GV+V GP  MRH+VAKICSS  A NL+F ++SF
Sbjct: 664 LFEAEGSEDVGVMVCGPKKMRHEVAKICSSGLAKNLHFEAISF 705

BLAST of Lag0020043 vs. ExPASy Swiss-Prot
Match: P92949 (Ferric reduction oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=FRO2 PE=1 SV=2)

HSP 1 Score: 667.5 bits (1721), Expect = 1.6e-190
Identity = 347/699 (49.64%), Postives = 481/699 (68.81%), Query Frame = 0

Query: 8   KMIFFLTFVLWLWLWIMLPTKLYKNTWTPELKMDL-NSTYFREQGTNLLLFSFPIMFMAV 67
           K +  + F+  + LWIM+PT  Y+  W P L++    STYF   GT L ++ FP+M +A 
Sbjct: 28  KFLMMVIFLGTIMLWIMMPTLTYRTKWLPHLRIKFGTSTYFGATGTTLFMYMFPMMVVAC 87

Query: 68  LGCFYLHLQKKSSEDPKPFSTQRSRGD-SFLRRPVLVMNSLGIVNTLEAMFAAAFVLLLI 127
           LGC YLH + + S  P     +   G  S LR+P+LV   LGIV+  E  F A FV LL+
Sbjct: 88  LGCVYLHFKNRKS--PHHIDRETKGGVWSKLRKPMLVKGPLGIVSVTEITFLAMFVALLL 147

Query: 128 WSLGNYLYVSFGHLHMHKVG---EKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRGSSLL 187
           W    YL  SF  +         E +W+AK  S +LRLG IGNIC AFLF PV RGSSLL
Sbjct: 148 WCFITYLRNSFATITPKSAAAHDESLWQAKLESAALRLGLIGNICLAFLFLPVARGSSLL 207

Query: 188 PLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQMALLIEWSVDYVSNVAGVI 247
           P  GLTSES+IKYHIWLGH+   LF +H + +I YWA   +++ +I W    VSN+AG I
Sbjct: 208 PAMGLTSESSIKYHIWLGHMVMALFTVHGLCYIIYWASMHEISQMIMWDTKGVSNLAGEI 267

Query: 248 AMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFLFAID 307
           A+A   V+W T++  IRR+ FEVF+Y+H+ YI++++F+++HVG  +  + LPG ++F +D
Sbjct: 268 ALAAGLVMWATTYPKIRRRFFEVFFYTHYLYIVFMLFFVLHVGISFSFIALPGFYIFLVD 327

Query: 308 RYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWHPFTV 367
           R+LRFLQS+  V L++AR LP   +EL F K+ +L YSPTS+MF+N+PSISK+QWHPFT+
Sbjct: 328 RFLRFLQSRENVRLLAARILPSDTMELTFSKNSKLVYSPTSIMFVNIPSISKLQWHPFTI 387

Query: 368 TSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSS--VDHLQVSVEGPYGPASTHFLRHESLL 427
           TS+ KLEP+ +S+VIK  G W++KLHQ+LSSS  +D L VSVEGPYGPAS  FLRHE+L+
Sbjct: 388 TSSSKLEPEKLSIVIKKEGKWSTKLHQRLSSSDQIDRLAVSVEGPYGPASADFLRHEALV 447

Query: 428 LISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPVSSTQTE 487
           ++ GGSGITPFIS+IR+ +I  S K  C +P+I LICAFK S +++MLDL+LP+S  +TE
Sbjct: 448 MVCGGSGITPFISVIRD-LIATSQKETCKIPKITLICAFKKSSEISMLDLVLPLSGLETE 507

Query: 488 I-SNVPIQIEAYITREKQQAETNAEKLINTKWFKPNPLDSPVSEALGPDNWLLLGAIISS 547
           + S++ I+IEA+ITR+    +      I T WFKP+  D  +S  LGP++WL LGAI++S
Sbjct: 508 LSSDINIKIEAFITRDNDAGDEAKAGKIKTLWFKPSLSDQSISSILGPNSWLWLGAILAS 567

Query: 548 SFVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSFVFLWERKTN 607
           SF++F+I++GIITRYY+YPI+ N + +Y+ + K +  + +    I  + S   LW +K  
Sbjct: 568 SFLIFMIIIGIITRYYIYPIDHNTNKIYSLTSKTIIYILVISVSIMATCSAAMLWNKKKY 627

Query: 608 AS-KHKQIQVLNVQSPKASP-GSWIYGGERELESLPCQSLVQATNIHYGGRPDLNKILLD 667
              + KQ+Q ++  SP +SP  SW Y   RE+ES P +SLVQ TN+H+G RP+L K+LLD
Sbjct: 628 GKVESKQVQNVDRPSPTSSPTSSWGYNSLREIESTPQESLVQRTNLHFGERPNLKKLLLD 687

Query: 668 CKGSDIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
            +GS +GVLV GP  MR  VA+ICSS  A+NL+F S+SF
Sbjct: 688 VEGSSVGVLVCGPKKMRQKVAEICSSGLAENLHFESISF 723

BLAST of Lag0020043 vs. ExPASy Swiss-Prot
Match: Q9LMM2 (Probable ferric reduction oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=FRO1 PE=2 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 1.2e-180
Identity = 333/706 (47.17%), Postives = 481/706 (68.13%), Query Frame = 0

Query: 8   KMIFFLTFVLWLW---LWIMLPTKLYKNTWTPELKMDL-NSTYFREQGTNLLLFSFPIMF 67
           K+I FL  V+ +    +WIM+PT  YK  W   ++  L  S Y+   G NLL++ FP++ 
Sbjct: 13  KVIKFLMMVILMGTIVIWIMMPTSTYKEIWLTSMRAKLGKSIYYGRPGVNLLVYMFPMIL 72

Query: 68  MAVLGCFYLHLQKKSSEDPKPFSTQRSRGD-SFLRRPVLVMNSLGIVNTLEAMFAAAFVL 127
           +A LGC YLHL+K ++ +      ++ R     LRRP+LV   LGIV   E MF   F+ 
Sbjct: 73  LAFLGCIYLHLKKSTTVNQFNSGVEKKRAKFGALRRPMLVNGPLGIVTVTEVMFLTMFMA 132

Query: 128 LLIWSLGNYLYVSFGHLHMHKV---GEKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRGS 187
           LL+WSL NY+Y +F ++        G  +W+A+   +++R+G +GNIC AFLF+PV RGS
Sbjct: 133 LLLWSLANYMYRTFVNVTSESAATDGNNLWQARLDLIAVRIGIVGNICLAFLFYPVARGS 192

Query: 188 SLLPLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQM-ALLIEWSVDYVSNV 247
           SLL   GLTSES+IKYHIWLGH+   +F  H + +  YW   +Q+ + ++EW    VSN+
Sbjct: 193 SLLAAVGLTSESSIKYHIWLGHLVMIIFTSHGLCYFIYWISKNQLVSKMLEWDRTAVSNL 252

Query: 248 AGVIAMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFL 307
           AG IA+    ++WVT++  IRR++FEVF+YSH+ YI++++F++ HVG  +  + LPG ++
Sbjct: 253 AGEIALVAGLMMWVTTYPKIRRRLFEVFFYSHYLYIVFMLFFVFHVGISHALIPLPGFYI 312

Query: 308 FAIDRYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWH 367
           F +DR+LRFLQS+  V LVSAR LPC  VELNF K+P L YSPTS MF+N+PSISK+QWH
Sbjct: 313 FLVDRFLRFLQSRNNVKLVSARVLPCDTVELNFSKNPMLMYSPTSTMFVNIPSISKLQWH 372

Query: 368 PFTVTSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSS----VDHLQVSVEGPYGPASTHFL 427
           PFT+ S+ KLEP+ +SV+IK+ G W++KL+  LSSS    ++ L VSVEGPYGP+ST FL
Sbjct: 373 PFTIISSSKLEPETLSVMIKSQGKWSTKLYDMLSSSSSDQINRLAVSVEGPYGPSSTDFL 432

Query: 428 RHESLLLISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPV 487
           RHESL+++SGGSGITPFISI+R++  + ST   C +P++ LICAFKNS DL+MLDL+LP 
Sbjct: 433 RHESLVMVSGGSGITPFISIVRDLFYMSSTH-KCKIPKMTLICAFKNSSDLSMLDLILPT 492

Query: 488 SSTQTEISN-VPIQIEAYITREKQ---QAETNAEKLINTKWFKPNPLDSPVSEALGPDNW 547
           S   T++++ V IQI+A++TRE++   +  T+   +I T+ FKPN  D P+S  LGP++W
Sbjct: 493 SGLTTDMASFVDIQIKAFVTREEKTSVKESTHNRNIIKTRHFKPNVSDQPISPILGPNSW 552

Query: 548 LLLGAIISSSFVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSF 607
           L L AI+SSSF++F++++ IITRY+++PI++N +  Y ++YK L  +      +  +S+ 
Sbjct: 553 LCLAAILSSSFMIFIVIIAIITRYHIHPIDQNSEK-YTWAYKSLIYLVSISITVVTTSTA 612

Query: 608 VFLWERKTNASKHKQIQVLNVQSPKASPGSWIYGGERELESLPCQSLVQATNIHYGGRPD 667
             LW +K   +K+   Q ++  SP              +ES P Q + Q+T+IHYG RP+
Sbjct: 613 AMLWNKKKYYAKND--QYVDNLSPVI------------IESSPQQLISQSTDIHYGERPN 672

Query: 668 LNKILLDCKGSDIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
           LNK+L+  KGS +G+LV GP  MR  VAKICS   A+NL+F S+SF
Sbjct: 673 LNKLLVGLKGSSVGILVCGPKKMRQKVAKICSFGSAENLHFESISF 702

BLAST of Lag0020043 vs. ExPASy Swiss-Prot
Match: F4I4K7 (Ferric reduction oxidase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FRO3 PE=2 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 5.7e-172
Identity = 332/700 (47.43%), Postives = 466/700 (66.57%), Query Frame = 0

Query: 7   LKMIFFLTFVLWLWLWIMLPTKLYKNTWTPELKMDL-NSTYFREQGTNLLLFSFPIMFMA 66
           +K++  +  +  + +WIM+PT  YK  W   ++  L  S YF + G NLL++ FP++ +A
Sbjct: 38  IKLLTMVILMGTVVIWIMMPTSTYKKIWLKSMRAKLGKSIYFGKPGVNLLVYMFPMILLA 97

Query: 67  VLGCFYLHLQKKSSEDPKPFSTQRSRGDSF--LRRPVLVMNSLGIVNTLEAMFAAAFVLL 126
            LG  YLHL+K++  +       R + D F  L+RP+LV   LGIV   E MF   F+ L
Sbjct: 98  SLGSIYLHLKKQTRVNQFNSRMDRKKIDKFGALKRPMLVKAGLGIVTVTEVMFLMMFMAL 157

Query: 127 LIWSLGNYLYVSFGHLHMHKV---GEKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRGSS 186
           L+WSL NY Y +F  +    +   G+ +W+A+  S+++RLG  GNIC  FLF+PV RGSS
Sbjct: 158 LLWSLANYFYHTFVTITPQSLPTDGDNLWQARLDSIAVRLGLTGNICLGFLFYPVARGSS 217

Query: 187 LLPLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQMALLIEWSVDYVSNVAG 246
           LL   GLTSES+ KYHIWLG++   LF  H + +  YW  T+Q++ ++EW    +S++AG
Sbjct: 218 LLAAVGLTSESSTKYHIWLGNLVMTLFTSHGLCYCIYWISTNQVSQMLEWDRTGISHLAG 277

Query: 247 VIAMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFLFA 306
            IA+    ++W T+F  IRR+ FEVF+Y+H+ Y+++++F++ HVG  Y  +  PG ++F 
Sbjct: 278 EIALVAGLLMWATTFPAIRRRFFEVFFYTHYLYMVFMLFFVFHVGISYALISFPGFYIFM 337

Query: 307 IDRYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWHPF 366
           +DR+LRFLQS+  V LVSAR LPC  VELNF K+P L YSPTS++F+N+PSISK+QWHPF
Sbjct: 338 VDRFLRFLQSRNNVKLVSARVLPCETVELNFSKNPMLMYSPTSILFVNIPSISKLQWHPF 397

Query: 367 TVTSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSS--VDHLQVSVEGPYGPASTHFLRHES 426
           T+TS+ KLEP  +SV+IK+ G W+SKLH  L+SS  +DHL VSVEGPYGPAST +LRH+S
Sbjct: 398 TITSSSKLEPKKLSVMIKSQGKWSSKLHHMLASSNQIDHLAVSVEGPYGPASTDYLRHDS 457

Query: 427 LLLISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPVSSTQ 486
           L+++SGGSGITPFISIIR+++ V ST      P+I LICAFKNS DL+ML+L+LP S   
Sbjct: 458 LVMVSGGSGITPFISIIRDLLYVSSTNA-YKTPKITLICAFKNSSDLSMLNLILPNS--- 517

Query: 487 TEISN-VPIQIEAYITREKQQAETNAEKLINTKWFKPNPLDSPVSEALGPDNWLLLGAII 546
           TEIS+ + IQI+A++TREK    T    +I T  FKP   D P+S  LGP++WL L  I+
Sbjct: 518 TEISSFIDIQIKAFVTREK--VSTCNMNIIKTLSFKPYVSDQPISPILGPNSWLWLATIL 577

Query: 547 SSSFVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSFVFLWERK 606
           SSSF++F+I++ II+RY++YPI+++    Y  +Y  L  +      +  +S+   L  +K
Sbjct: 578 SSSFMIFIIIIAIISRYHIYPIDQSSKE-YTSAYTSLIYLLAISISVVATSTVAMLCNKK 637

Query: 607 TNASKHKQI-QVLNVQSPKASPGSWIYGGERELESLPCQSLVQATNIHYGGRPDLNKILL 666
              S  K + Q ++  SP              +ES P Q L + TNIHYG RP+LNK+L+
Sbjct: 638 ---SYFKGLYQNVDALSPLM------------IESSPDQLLPEFTNIHYGERPNLNKLLV 697

Query: 667 DCKGSDIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
             KGS +GVLV GP  MR +VAKICS   A NL F S+SF
Sbjct: 698 GLKGSSVGVLVCGPRKMREEVAKICSFGSAANLQFESISF 715

BLAST of Lag0020043 vs. ExPASy TrEMBL
Match: A0A6J1E4P2 (ferric reduction oxidase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111430749 PE=4 SV=1)

HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 609/696 (87.50%), Postives = 656/696 (94.25%), Query Frame = 0

Query: 1   MAMARALKMIFFLTFVLWLWLWIMLPTKLYKNTWTPELKMDLNSTYFREQGTNLLLFSFP 60
           MAMAR +KM+F LTFVLWLWLWIMLPTKLYKNTWTP+L MDLNSTYFREQGTNLLLFSFP
Sbjct: 5   MAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLLFSFP 64

Query: 61  IMFMAVLGCFYLHLQKKSSEDPKPFSTQRSRGDSFLRRPVLVMNSLGIVNTLEAMFAAAF 120
           +M MAVLGCFYLHLQK SSEDPKPFSTQRSRGDSFL+RPVLVMN LGI+NTLE  FAAAF
Sbjct: 65  VMLMAVLGCFYLHLQKSSSEDPKPFSTQRSRGDSFLKRPVLVMNPLGILNTLEIGFAAAF 124

Query: 121 VLLLIWSLGNYLYVSFGHLHMHKVGEKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRGSS 180
           V+LLIWSLGNYLYVSFG+LHMH  GEK+WEAKFRSVSLRLGYIG+ICWAFLFFPVTRGSS
Sbjct: 125 VILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVTRGSS 184

Query: 181 LLPLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQMALLIEWSVDYVSNVAG 240
           LLP+ GLTSESTIKYHIWLGHISN LFALHTVGFI YW ITDQMALLIEWS DYVSN+AG
Sbjct: 185 LLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVSNIAG 244

Query: 241 VIAMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFLFA 300
           +IAMAFATVIWVTSFR IRRKMFEVF+YSHHFY LY +FYLIHVGA YVCL+LPG+FLFA
Sbjct: 245 LIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLVLPGMFLFA 304

Query: 301 IDRYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWHPF 360
           IDR+LRFLQSQR+VSLVSARHLPCGAVELN LKSPELSYSPTSVMF+NVP+ISK+QWHPF
Sbjct: 305 IDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSPELSYSPTSVMFLNVPTISKLQWHPF 364

Query: 361 TVTSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSSVDHLQVSVEGPYGPASTHFLRHESLL 420
           TVTSN KLEP I+SV IK GGSWTSKLHQQLSSS+DHLQVSVEGPYGPASTHFLRHESLL
Sbjct: 365 TVTSNSKLEPAIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTHFLRHESLL 424

Query: 421 LISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPVSSTQTE 480
           LISGGSGITPFISIIRE+MIVRSTKPNCHVPQ+RL+CAFKNSIDL MLDLLLPVSSTQTE
Sbjct: 425 LISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSSTQTE 484

Query: 481 ISNVPIQIEAYITREKQQAETNAEKLINTKWFKPNPLDSPVSEALGPDNWLLLGAIISSS 540
           ISNVP+QI+AYITRE++Q +TN E LINT WFKPNPLDSPV+EALGP+NWLLLGA+ISSS
Sbjct: 485 ISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPLDSPVTEALGPNNWLLLGAVISSS 544

Query: 541 FVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSFVFLWERKTNA 600
           FVMFLILLGIITRYY+YPIERNGDA+YNY+YKVLWDMFL+ ACICI SS V +W+RKT  
Sbjct: 545 FVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQRKTYV 604

Query: 601 SKHKQIQVLNVQSPKASPGSWIYGGERELESLPCQSLVQATNIHYGGRPDLNKILLDCKG 660
           S +KQ+QVLNVQSPKASPGSWI+GGERELESLP Q LVQATNIHYGGRPDL +ILLDCKG
Sbjct: 605 S-NKQVQVLNVQSPKASPGSWIFGGERELESLPFQPLVQATNIHYGGRPDLKRILLDCKG 664

Query: 661 SDIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
           SDIGVLVSGPTGMRHDVAKICS+AY DNLNFH+MSF
Sbjct: 665 SDIGVLVSGPTGMRHDVAKICSTAYGDNLNFHAMSF 699

BLAST of Lag0020043 vs. ExPASy TrEMBL
Match: A0A6J1JC03 (ferric reduction oxidase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111483082 PE=4 SV=1)

HSP 1 Score: 1235.7 bits (3196), Expect = 0.0e+00
Identity = 604/696 (86.78%), Postives = 651/696 (93.53%), Query Frame = 0

Query: 1   MAMARALKMIFFLTFVLWLWLWIMLPTKLYKNTWTPELKMDLNSTYFREQGTNLLLFSFP 60
           MAMAR +KM+F LTFVLWLWLWIMLPTKLYKNTWTP+L MDLNSTYFREQGTNLLLFSFP
Sbjct: 5   MAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLLFSFP 64

Query: 61  IMFMAVLGCFYLHLQKKSSEDPKPFSTQRSRGDSFLRRPVLVMNSLGIVNTLEAMFAAAF 120
           +M MAVLGCFYL LQK SSEDPKPFSTQRSRGDSFL+RPVL MN LGI+NTLE  FAAAF
Sbjct: 65  VMLMAVLGCFYLQLQKSSSEDPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIGFAAAF 124

Query: 121 VLLLIWSLGNYLYVSFGHLHMHKVGEKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRGSS 180
           V+LLIWSLGNYLYVSFG+LHMH  GEK+WEAKFRSVSLRLGYIG+ICWAFLFFPVTRGSS
Sbjct: 125 VILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVTRGSS 184

Query: 181 LLPLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQMALLIEWSVDYVSNVAG 240
           LLP+ GLTSESTIKYHIWLGHISN LFALHTVGFI YW ITDQMALLIEWS DYVSN+AG
Sbjct: 185 LLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVSNIAG 244

Query: 241 VIAMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFLFA 300
           +IAMAFATVIWVTSFR IRRKMFEVF+YSHHFY LY +FYLIHVGA YVCLILPG+FLFA
Sbjct: 245 LIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGMFLFA 304

Query: 301 IDRYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWHPF 360
           IDR+LRFLQSQR+VSLVSARHLPCGAVELN LKS ELSYSPTSVMF+NVP+ISK+QWHPF
Sbjct: 305 IDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQWHPF 364

Query: 361 TVTSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSSVDHLQVSVEGPYGPASTHFLRHESLL 420
           T+TSN KLEPDI+SV IK GGSWTSKLHQQLSSS+DHLQVSVEGPYGPAST FLRHESLL
Sbjct: 365 TITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLRHESLL 424

Query: 421 LISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPVSSTQTE 480
           LISGGSGITPFISIIRE+MIVRSTKPNCHVPQ+RL+CAFKNSIDL MLDLLLPVSSTQTE
Sbjct: 425 LISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSSTQTE 484

Query: 481 ISNVPIQIEAYITREKQQAETNAEKLINTKWFKPNPLDSPVSEALGPDNWLLLGAIISSS 540
           ISNVP+QI+AYITRE++Q +TN E LINT WFKPNP+DSPVSEALGP+NWLLLG +ISSS
Sbjct: 485 ISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLLGTVISSS 544

Query: 541 FVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSFVFLWERKTNA 600
           FVMFLILLGIITRYY+YPIERNGDA+YNY+YKVLWDMFL+ ACICI SS V +W+RKT  
Sbjct: 545 FVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQRKTYV 604

Query: 601 SKHKQIQVLNVQSPKASPGSWIYGGERELESLPCQSLVQATNIHYGGRPDLNKILLDCKG 660
           S +KQ+QVLNVQSPKASPGSWI+GGERELESLP Q LVQATNIHYGGRPDL +ILLDCKG
Sbjct: 605 S-NKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLDCKG 664

Query: 661 SDIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
           SDIGVLVSGP GMRHDVAKICS+AY DNLNFH+MSF
Sbjct: 665 SDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSF 699

BLAST of Lag0020043 vs. ExPASy TrEMBL
Match: A0A6J1E5E9 (ferric reduction oxidase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111430747 PE=4 SV=1)

HSP 1 Score: 1228.0 bits (3176), Expect = 0.0e+00
Identity = 605/696 (86.93%), Postives = 651/696 (93.53%), Query Frame = 0

Query: 1   MAMARALKMIFFLTFVLWLWLWIMLPTKLYKNTWTPELKMDLNSTYFREQGTNLLLFSFP 60
           MAMARALKMIF LTFVLWLWLWIMLPTKLYKNTWTP+LKM+LNSTYFREQGTNLLLFSFP
Sbjct: 5   MAMARALKMIFLLTFVLWLWLWIMLPTKLYKNTWTPKLKMELNSTYFREQGTNLLLFSFP 64

Query: 61  IMFMAVLGCFYLHLQKKSSEDPKPFSTQRSRGDSFLRRPVLVMNSLGIVNTLEAMFAAAF 120
           IMFMAVLGCFYLHLQK SSED KPFSTQRSRGDSFL+RP+L MN LGIVNTLE  FAAAF
Sbjct: 65  IMFMAVLGCFYLHLQKNSSEDSKPFSTQRSRGDSFLKRPILAMNPLGIVNTLEIGFAAAF 124

Query: 121 VLLLIWSLGNYLYVSFGHLHMHKVGEKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRGSS 180
           V+LLIWSLGNYLYVSFG+LHMH  GEKIWEAKFRSVSLRLGYIG+ICWAFLFFPVTRGSS
Sbjct: 125 VILLIWSLGNYLYVSFGNLHMHGAGEKIWEAKFRSVSLRLGYIGHICWAFLFFPVTRGSS 184

Query: 181 LLPLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQMALLIEWSVDYVSNVAG 240
           LLPL GLTSESTIKYHIWLGHISN LFALHTVGFI YW ITDQMALL EWS  YVSNVAG
Sbjct: 185 LLPLIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLKEWSEVYVSNVAG 244

Query: 241 VIAMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFLFA 300
           +IAMAFATVIWVTSFR IR+KMFEVF+YSHH Y LY +FYLIHVGA YVC+ILPG+FLFA
Sbjct: 245 LIAMAFATVIWVTSFRSIRQKMFEVFFYSHHLYTLYTVFYLIHVGAAYVCMILPGMFLFA 304

Query: 301 IDRYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWHPF 360
           IDR+LRFLQSQR VSLVSARHLPCG VELN LKSPELSYSPTSVMF+NVPSISK+QWHPF
Sbjct: 305 IDRFLRFLQSQRHVSLVSARHLPCGVVELNLLKSPELSYSPTSVMFLNVPSISKLQWHPF 364

Query: 361 TVTSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSSVDHLQVSVEGPYGPASTHFLRHESLL 420
           T+TSN KLEPDIVSVV K GGSWTSKLHQQLSSS+DHLQVSVEGPYGPASTHFLRHESLL
Sbjct: 365 TITSNSKLEPDIVSVVSKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTHFLRHESLL 424

Query: 421 LISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPVSSTQTE 480
           LISGGSGITPFISIIRE+MIVRSTKP+CHVPQ+RLICAFKNS+DL MLDLLLPVSSTQTE
Sbjct: 425 LISGGSGITPFISIIREIMIVRSTKPDCHVPQVRLICAFKNSMDLTMLDLLLPVSSTQTE 484

Query: 481 ISNVPIQIEAYITREKQQAETNAEKLINTKWFKPNPLDSPVSEALGPDNWLLLGAIISSS 540
           ISNVP+QIEAYITRE++  +TN E L+NT WFKPNPLDSPVSEALGP++WLLLGA+ISSS
Sbjct: 485 ISNVPLQIEAYITREEEPPQTNEENLVNTIWFKPNPLDSPVSEALGPNHWLLLGAVISSS 544

Query: 541 FVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSFVFLWERKTNA 600
           F+MFLILLGIITRYY+YPIERNGDA+YNY+YKVLWDMFLA ACIC+SSSFV +W+RKT  
Sbjct: 545 FMMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLALACICVSSSFVLMWQRKTYV 604

Query: 601 SKHKQIQVLNVQSPKASPGSWIYGGERELESLPCQSLVQATNIHYGGRPDLNKILLDCKG 660
           S +K++QVL+VQSPKASPGSWIYGGERELESLP QSLVQATN+HYG RPDL KILLDCKG
Sbjct: 605 S-NKRVQVLSVQSPKASPGSWIYGGERELESLPYQSLVQATNVHYGSRPDLKKILLDCKG 664

Query: 661 SDIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
           SDIGVLVSGPT +RHDVAKICS+A  DNL FH++SF
Sbjct: 665 SDIGVLVSGPTRLRHDVAKICSTACGDNLKFHALSF 699

BLAST of Lag0020043 vs. ExPASy TrEMBL
Match: A0A1S3AZ42 (ferric reduction oxidase 4-like OS=Cucumis melo OX=3656 GN=LOC103484121 PE=4 SV=1)

HSP 1 Score: 1218.4 bits (3151), Expect = 0.0e+00
Identity = 605/698 (86.68%), Postives = 649/698 (92.98%), Query Frame = 0

Query: 1   MAMARALKMIFFLTFVLWLWLWIMLPTKLYKNTWTPELKMDLNSTYFREQGTNLLLFSFP 60
           +AM RALKMIFFLTFVLWLWLWIMLPTKLYKN W+P+L M+LNSTYFREQGTNLLLFSFP
Sbjct: 5   IAMGRALKMIFFLTFVLWLWLWIMLPTKLYKNYWSPKLNMNLNSTYFREQGTNLLLFSFP 64

Query: 61  IMFMAVLGCFYLHLQKKSSE-DPKPFSTQRSRGDSFLRRPVLVMNSLGIVNTLEAMFAAA 120
           IM MAVLGCFYLH QK+SS+ DPKPFSTQR RG+SFL+RPV+VMN LGIVNTLE +F  +
Sbjct: 65  IMLMAVLGCFYLHFQKESSDLDPKPFSTQRVRGESFLKRPVVVMNPLGIVNTLEIVFGVS 124

Query: 121 FVLLLIWSLGNYLYVSFGHLHMHKVGEKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRGS 180
           F+LLLIWSLGNYLYVSFGHLH   VGE I E KFRSVSLR+GYIGNICWAFLFFPVTRGS
Sbjct: 125 FLLLLIWSLGNYLYVSFGHLHRDNVGETIGELKFRSVSLRVGYIGNICWAFLFFPVTRGS 184

Query: 181 SLLPLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQMALLIEWSVDYVSNVA 240
           SLLPL GLTSES+IKYHIWLGH+SN LFALHT+GFI YWA+TDQMALL +WS DYVSNVA
Sbjct: 185 SLLPLIGLTSESSIKYHIWLGHLSNLLFALHTIGFIIYWALTDQMALLKQWSEDYVSNVA 244

Query: 241 GVIAMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFLF 300
           GVIAM FAT+IWVTSF+ IRRKMFEVFYY+HHFYILYIIFYLIHVG  YVCLILPGIFL+
Sbjct: 245 GVIAMTFATIIWVTSFQSIRRKMFEVFYYTHHFYILYIIFYLIHVGVAYVCLILPGIFLW 304

Query: 301 AIDRYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWHP 360
            IDRYLRFLQSQR+VSLVSARHLPCG VELNFLKSPELSY+PTSVMFINVPSISK+QWHP
Sbjct: 305 FIDRYLRFLQSQRRVSLVSARHLPCGVVELNFLKSPELSYNPTSVMFINVPSISKLQWHP 364

Query: 361 FTVTSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSSVDHLQVSVEGPYGPASTHFLRHESL 420
           FTVTSNCKLEPDIVSVVIK+GGSWTSKLH+ LSSS+DHLQVSVEGPYGPASTHFLRHE L
Sbjct: 365 FTVTSNCKLEPDIVSVVIKSGGSWTSKLHKHLSSSLDHLQVSVEGPYGPASTHFLRHEYL 424

Query: 421 LLISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPVSSTQT 480
           LLISGGSGITPFISIIRE+MIVRSTK N HVPQIRLICAFKNSIDL MLDLLLPVSSTQT
Sbjct: 425 LLISGGSGITPFISIIREIMIVRSTKVNFHVPQIRLICAFKNSIDLTMLDLLLPVSSTQT 484

Query: 481 E-ISNVPIQIEAYITREKQQAETNAEKLINTKWFKPNPLDSPVSEALGPDNWLLLGAIIS 540
           E ISNVP+QIEAYITREK+Q +TN+E LINT WFKPNPLDSP+SEALG ++WLLLGAIIS
Sbjct: 485 ELISNVPLQIEAYITREKEQPQTNSENLINTIWFKPNPLDSPISEALGRNSWLLLGAIIS 544

Query: 541 SSFVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSFVFLWERKT 600
           SSFVMFLILLGI TRYY+YPIE+NGD +YNY+YKVLWDMFLAFACICISSSFVFLW+RKT
Sbjct: 545 SSFVMFLILLGITTRYYIYPIEQNGDVVYNYTYKVLWDMFLAFACICISSSFVFLWQRKT 604

Query: 601 NASKHKQIQVLNVQSPKASPGSWIYGGERELESLPCQSLVQATNIHYGGRPDLNKILLDC 660
           NAS  KQIQ+ N QSPKASPGSWI+GG+ ELESLP QSLVQATNIHYGGRPDLNKIL DC
Sbjct: 605 NASNDKQIQIHNFQSPKASPGSWIFGGDSELESLPYQSLVQATNIHYGGRPDLNKILFDC 664

Query: 661 KGSDIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
           KGSDIGVLVSGPTGMRHDVAKICSSAY DNL FHSMSF
Sbjct: 665 KGSDIGVLVSGPTGMRHDVAKICSSAYGDNLKFHSMSF 702

BLAST of Lag0020043 vs. ExPASy TrEMBL
Match: A0A0A0L8S6 (FAD-binding FR-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G183380 PE=4 SV=1)

HSP 1 Score: 1216.1 bits (3145), Expect = 0.0e+00
Identity = 603/697 (86.51%), Postives = 645/697 (92.54%), Query Frame = 0

Query: 1   MAMARALKMIFFLTFVLWLWLWIMLPTKLYKNTWTPELKMDLNSTYFREQGTNLLLFSFP 60
           MAM RALK+IFFLTFVLWLWLWIMLPTKLYKN WTP+L M+LNST+FREQGTNLLLFSFP
Sbjct: 5   MAMGRALKIIFFLTFVLWLWLWIMLPTKLYKNYWTPKLNMNLNSTFFREQGTNLLLFSFP 64

Query: 61  IMFMAVLGCFYLHLQKKSSE-DPKPFSTQRSRGDSFLRRPVLVMNSLGIVNTLEAMFAAA 120
           IM MAVLGCFYLH QKKSSE DPKPFSTQR RG+SFL+RPV+VMN LGIVNTLE MF  +
Sbjct: 65  IMLMAVLGCFYLHFQKKSSELDPKPFSTQRVRGESFLKRPVVVMNPLGIVNTLEIMFGVS 124

Query: 121 FVLLLIWSLGNYLYVSFGHLHMHKVGEKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRGS 180
           F+LLLIWSLGNYLYVSFGHLH   VGE IWE KFRSVSLR+GYIGNICWAFLFFPVTRGS
Sbjct: 125 FLLLLIWSLGNYLYVSFGHLHRDNVGETIWELKFRSVSLRVGYIGNICWAFLFFPVTRGS 184

Query: 181 SLLPLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQMALLIEWSVDYVSNVA 240
           SLLPL GLTSES+IKYHIWLGH+SN LFALHT+GFI YWA+T Q+ALL +WS DYVSNVA
Sbjct: 185 SLLPLIGLTSESSIKYHIWLGHLSNLLFALHTIGFIIYWALTHQLALLKQWSEDYVSNVA 244

Query: 241 GVIAMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFLF 300
           GVIAMAFAT+IWVTS + IR KMFEVFYY+HH YILYIIFYLIHVG  YVCLILPGIFL+
Sbjct: 245 GVIAMAFATIIWVTSLQSIRPKMFEVFYYTHHLYILYIIFYLIHVGVAYVCLILPGIFLW 304

Query: 301 AIDRYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWHP 360
           AIDRYLRFLQSQR+VSLVSARHLP GAVELNFLKSPELSY+PTSVMFINVPSISK+QWHP
Sbjct: 305 AIDRYLRFLQSQRRVSLVSARHLPSGAVELNFLKSPELSYNPTSVMFINVPSISKLQWHP 364

Query: 361 FTVTSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSSVDHLQVSVEGPYGPASTHFLRHESL 420
           FTVTSNCKLEPDIVSVVIK+GGSWTSKLH+QLSSS+DHLQVSVEGPYGPASTHFLRHESL
Sbjct: 365 FTVTSNCKLEPDIVSVVIKSGGSWTSKLHKQLSSSLDHLQVSVEGPYGPASTHFLRHESL 424

Query: 421 LLISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPVSSTQT 480
           LLISGGSGITPFISII+E+MIVRST  N HVPQIRLICAFK SIDL MLDLLLPVSSTQT
Sbjct: 425 LLISGGSGITPFISIIKEIMIVRSTNINFHVPQIRLICAFKKSIDLTMLDLLLPVSSTQT 484

Query: 481 EISNVPIQIEAYITREKQQAETNAEKLINTKWFKPNPLDSPVSEALGPDNWLLLGAIISS 540
           EISNVP+ IEAYITREK+Q +TN+E LINT WFKPNPLDSPVSE LG +NWLLLGAIISS
Sbjct: 485 EISNVPLHIEAYITREKEQPQTNSENLINTIWFKPNPLDSPVSEVLGRNNWLLLGAIISS 544

Query: 541 SFVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSFVFLWERKTN 600
           SFVMFLILLGI+TRYY+YPIERNGDA+YNY+YKVLWDMFLAFACIC SSS VFLW+RKTN
Sbjct: 545 SFVMFLILLGIVTRYYIYPIERNGDAVYNYTYKVLWDMFLAFACICFSSSLVFLWQRKTN 604

Query: 601 ASKHKQIQVLNVQSPKASPGSWIYGGERELESLPCQSLVQATNIHYGGRPDLNKILLDCK 660
           AS  K+IQ+ N QSPKASPGSWI+GG+ ELESLP QSLVQATNIHY GRPDLNKIL DCK
Sbjct: 605 ASNDKKIQIHNFQSPKASPGSWIFGGDSELESLPYQSLVQATNIHYEGRPDLNKILFDCK 664

Query: 661 GSDIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
           GSDIGVLVSGPTGMRHDVAKICSSAY DNL FHSMSF
Sbjct: 665 GSDIGVLVSGPTGMRHDVAKICSSAYGDNLKFHSMSF 701

BLAST of Lag0020043 vs. TAIR 10
Match: AT5G23980.1 (ferric reduction oxidase 4 )

HSP 1 Score: 787.7 bits (2033), Expect = 7.5e-228
Identity = 391/701 (55.78%), Postives = 519/701 (74.04%), Query Frame = 0

Query: 3   MARALKMIFFLTFVLWLWLWIMLPTKLYKNTWTPELKMDLNSTYFREQGTNLLLFSFPIM 62
           M   +K +  + F+ W+ +WIM+ T L+K+ WTP+L   LN+TYF  QGTNL+L + P+M
Sbjct: 4   MRSLVKTLMVVLFLGWILVWIMISTNLFKSKWTPKLSKYLNTTYFGPQGTNLVLLTVPMM 63

Query: 63  FMAVLGCFYLHLQKKSSEDPKPFSTQRSRGDSFLRRPVLVMNSLGIVNTLEAMFAAAFVL 122
           F+AVL C YLH+QKK ++  + +  +R  G     R ++VMN LGIV   E  F+  FV 
Sbjct: 64  FIAVLSCVYLHIQKKPTQPQREWKLKRIMG-----RVIMVMNPLGIVTATELTFSLLFVA 123

Query: 123 LLIWSLGNYLYVSFGHLHMHK-VGEKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRGSSL 182
           LL WSL NYLY+S+ H+H+H     KIW+AKFR+  LR+GY+GNICWAFLFFPVTR S++
Sbjct: 124 LLAWSLYNYLYLSY-HVHLHNDDNAKIWQAKFRAFGLRIGYVGNICWAFLFFPVTRASTI 183

Query: 183 LPLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQMALLIEWSVDYVSNVAGV 242
           LPL GLTSES+IKYHIWLGH+SNF F +HTV F+ YWA+ +++     W+  YV N+AG 
Sbjct: 184 LPLVGLTSESSIKYHIWLGHVSNFCFLVHTVVFLIYWAMINKLMETFAWNPTYVPNLAGT 243

Query: 243 IAMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFLFAI 302
           IAM     +WVTS    RRK FE+F+Y+HH Y LYI+FY+IHVG  + C+ILP IFLF I
Sbjct: 244 IAMVIGIAMWVTSLPSFRRKKFEIFFYTHHLYGLYIVFYVIHVGDSWFCMILPNIFLFFI 303

Query: 303 DRYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWHPFT 362
           DRYLRFLQS ++  LVSAR LP   +EL F K+P L Y+PTS++F++VPSISK+QWHPFT
Sbjct: 304 DRYLRFLQSTKRSRLVSARILPSDNLELTFSKTPGLHYTPTSILFLHVPSISKIQWHPFT 363

Query: 363 VTSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSSVDHLQVSVEGPYGPASTHFLRHESLLL 422
           +TS+  LE D +SVVI+  GSWT KL+  LSSS+D L+VS EGPYGP S    RH SL+L
Sbjct: 364 ITSSSNLEKDTLSVVIRRQGSWTQKLYTHLSSSIDSLEVSTEGPYGPNSFDVSRHNSLIL 423

Query: 423 ISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPVSSTQTEI 482
           +SGGSGITPFIS+IRE +I +S   +  +P + L+C+FK+  DL  LDL+ P+  + ++I
Sbjct: 424 VSGGSGITPFISVIRE-LISQSQNKSTKLPDVLLVCSFKHYHDLAFLDLIFPLDMSASDI 483

Query: 483 SNVPIQIEAYITREKQQAETNAE-KLINTKWFKPNPLDSPVSEALGPDNWLLLGAIISSS 542
           S + ++IEAYITRE ++ ET  + +L+ TKWFKP PLDSP+S  LGP+N+L LG +I SS
Sbjct: 484 SRLNLRIEAYITREDKKPETTDDHRLLQTKWFKPQPLDSPISPVLGPNNFLWLGVVILSS 543

Query: 543 FVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSFVFLWERKTNA 602
           FVMFL+L+GI+TRYY+YP++ N  ++YN+SY+ LWDMFL  ACI ISSS VFLW +K N 
Sbjct: 544 FVMFLLLIGIVTRYYIYPVDHNTGSIYNFSYRGLWDMFLGSACIFISSSVVFLWRKKQNK 603

Query: 603 SKHK----QIQVLNVQSPKASPGSWIYGGERELESLPCQSLVQATNIHYGGRPDLNKILL 662
              K    Q+Q +  Q+P +SPGSW +G ERELES+P QS+VQAT++H+G +P+L KILL
Sbjct: 604 EGDKEFKNQVQSVEFQTPTSSPGSWFHGHERELESVPYQSIVQATSVHFGSKPNLKKILL 663

Query: 663 DCKGS-DIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
           + +GS D+GV+V GP  MRH+VAKICSS  A NL+F ++SF
Sbjct: 664 EAEGSEDVGVMVCGPRKMRHEVAKICSSGLAKNLHFEAISF 697

BLAST of Lag0020043 vs. TAIR 10
Match: AT5G23990.1 (ferric reduction oxidase 5 )

HSP 1 Score: 667.9 bits (1722), Expect = 8.7e-192
Identity = 347/704 (49.29%), Postives = 470/704 (66.76%), Query Frame = 0

Query: 3   MARALKMIFFLTFVLWLWLWIMLPTKLYKNTWTPEL-KMDLNSTYFREQ---GTNLLLFS 62
           M   +KM+  + F+ W+++WIM+ T  ++N WTP+L K +  +T+       G+ L LF+
Sbjct: 4   MRSLVKMLMVVLFLGWIFVWIMISTNRFQNIWTPKLAKHESCATHGSNDVHCGSELCLFA 63

Query: 63  FPIMFMAVLGCFYLHLQKKSSEDPKPFSTQRSRGDSFLRRPVLVMNSLGIVNTLEAMFAA 122
           +P               K +  + K    +R  G+                         
Sbjct: 64  YP---------------KATLPNSKRMEGKRENGE------------------------- 123

Query: 123 AFVLLLIWSLGNYLYVSFGHLHMHKVGEKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRG 182
                     G+  Y   G    H+V +    AKFR+  LR+GY+G+ CWAFLFFPVTR 
Sbjct: 124 ----------GDDGYEPIGDSDSHRV-DFFSVAKFRAFGLRIGYVGHYCWAFLFFPVTRA 183

Query: 183 SSLLPLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQMALLIEWSVDYVSNV 242
           S++LPL GLTSES+IKYHIWLGH+SNF F +HTV F+ YWA+ +++     W+  YV N+
Sbjct: 184 STILPLVGLTSESSIKYHIWLGHVSNFCFLVHTVVFLIYWAMVNKLMETFAWNATYVPNL 243

Query: 243 AGVIAMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFL 302
           AG IAM     IWVTS    RRK FE+F+Y+HH Y LYI+FY IHVG  + C+ILP IFL
Sbjct: 244 AGTIAMVIGIAIWVTSLPSFRRKKFEIFFYTHHLYGLYIVFYAIHVGDSWFCMILPNIFL 303

Query: 303 FAIDRYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWH 362
           F IDRYLRFLQS ++  LVSA+ LP   +EL F K+  L Y+PTS++F++VPSISK+QWH
Sbjct: 304 FFIDRYLRFLQSTKRSRLVSAKILPSDNLELTFAKTSGLHYTPTSILFLHVPSISKLQWH 363

Query: 363 PFTVTSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSSVDHLQVSVEGPYGPASTHFLRHES 422
           PFT+TS+  LE D +SVVI+  GSWT KL+  LSSS+D L+VS EGPYGP S    RH+S
Sbjct: 364 PFTITSSSNLEKDTLSVVIRKQGSWTQKLYTHLSSSIDSLEVSTEGPYGPNSFDVSRHDS 423

Query: 423 LLLISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPVSSTQ 482
           L+L+ GGSG+TPFIS+IRE +I +S   +  +P + L+CAFKN  DL  LDL+ P   + 
Sbjct: 424 LILVGGGSGVTPFISVIRE-LIFQSQNRSTKLPNVLLVCAFKNYHDLAFLDLIFPSDISV 483

Query: 483 TEISNVPIQIEAYITREKQQAETNAE-KLINTKWFKPNPLDSPVSEALGPDNWLLLGAII 542
           ++IS + ++IEAYITRE ++ ET  + KL+ TKWFKP PLDSP+S  LGP+N+L LG +I
Sbjct: 484 SDISKLNLRIEAYITREDKKPETTDDHKLLQTKWFKPQPLDSPISPVLGPNNFLWLGVVI 543

Query: 543 SSSFVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSFVFLWERK 602
            SSFVMFL+L+GI+TRYY+YP++ N  ++YN++Y+VLW MFL   CI ISSS +FLW +K
Sbjct: 544 LSSFVMFLLLIGIVTRYYIYPVDHNTGSIYNFTYRVLWVMFLGCVCIFISSSIIFLWRKK 603

Query: 603 TN----ASKHKQIQVLNVQSPKASPGSWIYGGERELESLPCQSLVQATNIHYGGRPDLNK 662
            N        KQ+Q +  Q+P +SPGSW +G ERELES+P QS+VQAT++H+G +P+L K
Sbjct: 604 ENKEGDKDSKKQVQSVEFQTPTSSPGSWFHGHERELESVPYQSIVQATSVHFGSKPNLKK 655

Query: 663 ILLDCKGS-DIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
           IL + +GS D+GV+V GP  MRH+VAKICSS  A NL+F ++SF
Sbjct: 664 ILFEAEGSEDVGVMVCGPKKMRHEVAKICSSGLAKNLHFEAISF 655

BLAST of Lag0020043 vs. TAIR 10
Match: AT1G01580.1 (ferric reduction oxidase 2 )

HSP 1 Score: 667.5 bits (1721), Expect = 1.1e-191
Identity = 347/699 (49.64%), Postives = 481/699 (68.81%), Query Frame = 0

Query: 8   KMIFFLTFVLWLWLWIMLPTKLYKNTWTPELKMDL-NSTYFREQGTNLLLFSFPIMFMAV 67
           K +  + F+  + LWIM+PT  Y+  W P L++    STYF   GT L ++ FP+M +A 
Sbjct: 28  KFLMMVIFLGTIMLWIMMPTLTYRTKWLPHLRIKFGTSTYFGATGTTLFMYMFPMMVVAC 87

Query: 68  LGCFYLHLQKKSSEDPKPFSTQRSRGD-SFLRRPVLVMNSLGIVNTLEAMFAAAFVLLLI 127
           LGC YLH + + S  P     +   G  S LR+P+LV   LGIV+  E  F A FV LL+
Sbjct: 88  LGCVYLHFKNRKS--PHHIDRETKGGVWSKLRKPMLVKGPLGIVSVTEITFLAMFVALLL 147

Query: 128 WSLGNYLYVSFGHLHMHKVG---EKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRGSSLL 187
           W    YL  SF  +         E +W+AK  S +LRLG IGNIC AFLF PV RGSSLL
Sbjct: 148 WCFITYLRNSFATITPKSAAAHDESLWQAKLESAALRLGLIGNICLAFLFLPVARGSSLL 207

Query: 188 PLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQMALLIEWSVDYVSNVAGVI 247
           P  GLTSES+IKYHIWLGH+   LF +H + +I YWA   +++ +I W    VSN+AG I
Sbjct: 208 PAMGLTSESSIKYHIWLGHMVMALFTVHGLCYIIYWASMHEISQMIMWDTKGVSNLAGEI 267

Query: 248 AMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFLFAID 307
           A+A   V+W T++  IRR+ FEVF+Y+H+ YI++++F+++HVG  +  + LPG ++F +D
Sbjct: 268 ALAAGLVMWATTYPKIRRRFFEVFFYTHYLYIVFMLFFVLHVGISFSFIALPGFYIFLVD 327

Query: 308 RYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWHPFTV 367
           R+LRFLQS+  V L++AR LP   +EL F K+ +L YSPTS+MF+N+PSISK+QWHPFT+
Sbjct: 328 RFLRFLQSRENVRLLAARILPSDTMELTFSKNSKLVYSPTSIMFVNIPSISKLQWHPFTI 387

Query: 368 TSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSS--VDHLQVSVEGPYGPASTHFLRHESLL 427
           TS+ KLEP+ +S+VIK  G W++KLHQ+LSSS  +D L VSVEGPYGPAS  FLRHE+L+
Sbjct: 388 TSSSKLEPEKLSIVIKKEGKWSTKLHQRLSSSDQIDRLAVSVEGPYGPASADFLRHEALV 447

Query: 428 LISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPVSSTQTE 487
           ++ GGSGITPFIS+IR+ +I  S K  C +P+I LICAFK S +++MLDL+LP+S  +TE
Sbjct: 448 MVCGGSGITPFISVIRD-LIATSQKETCKIPKITLICAFKKSSEISMLDLVLPLSGLETE 507

Query: 488 I-SNVPIQIEAYITREKQQAETNAEKLINTKWFKPNPLDSPVSEALGPDNWLLLGAIISS 547
           + S++ I+IEA+ITR+    +      I T WFKP+  D  +S  LGP++WL LGAI++S
Sbjct: 508 LSSDINIKIEAFITRDNDAGDEAKAGKIKTLWFKPSLSDQSISSILGPNSWLWLGAILAS 567

Query: 548 SFVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSFVFLWERKTN 607
           SF++F+I++GIITRYY+YPI+ N + +Y+ + K +  + +    I  + S   LW +K  
Sbjct: 568 SFLIFMIIIGIITRYYIYPIDHNTNKIYSLTSKTIIYILVISVSIMATCSAAMLWNKKKY 627

Query: 608 AS-KHKQIQVLNVQSPKASP-GSWIYGGERELESLPCQSLVQATNIHYGGRPDLNKILLD 667
              + KQ+Q ++  SP +SP  SW Y   RE+ES P +SLVQ TN+H+G RP+L K+LLD
Sbjct: 628 GKVESKQVQNVDRPSPTSSPTSSWGYNSLREIESTPQESLVQRTNLHFGERPNLKKLLLD 687

Query: 668 CKGSDIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
            +GS +GVLV GP  MR  VA+ICSS  A+NL+F S+SF
Sbjct: 688 VEGSSVGVLVCGPKKMRQKVAEICSSGLAENLHFESISF 723

BLAST of Lag0020043 vs. TAIR 10
Match: AT1G01590.1 (ferric reduction oxidase 1 )

HSP 1 Score: 634.8 bits (1636), Expect = 8.2e-182
Identity = 333/706 (47.17%), Postives = 481/706 (68.13%), Query Frame = 0

Query: 8   KMIFFLTFVLWLW---LWIMLPTKLYKNTWTPELKMDL-NSTYFREQGTNLLLFSFPIMF 67
           K+I FL  V+ +    +WIM+PT  YK  W   ++  L  S Y+   G NLL++ FP++ 
Sbjct: 13  KVIKFLMMVILMGTIVIWIMMPTSTYKEIWLTSMRAKLGKSIYYGRPGVNLLVYMFPMIL 72

Query: 68  MAVLGCFYLHLQKKSSEDPKPFSTQRSRGD-SFLRRPVLVMNSLGIVNTLEAMFAAAFVL 127
           +A LGC YLHL+K ++ +      ++ R     LRRP+LV   LGIV   E MF   F+ 
Sbjct: 73  LAFLGCIYLHLKKSTTVNQFNSGVEKKRAKFGALRRPMLVNGPLGIVTVTEVMFLTMFMA 132

Query: 128 LLIWSLGNYLYVSFGHLHMHKV---GEKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRGS 187
           LL+WSL NY+Y +F ++        G  +W+A+   +++R+G +GNIC AFLF+PV RGS
Sbjct: 133 LLLWSLANYMYRTFVNVTSESAATDGNNLWQARLDLIAVRIGIVGNICLAFLFYPVARGS 192

Query: 188 SLLPLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQM-ALLIEWSVDYVSNV 247
           SLL   GLTSES+IKYHIWLGH+   +F  H + +  YW   +Q+ + ++EW    VSN+
Sbjct: 193 SLLAAVGLTSESSIKYHIWLGHLVMIIFTSHGLCYFIYWISKNQLVSKMLEWDRTAVSNL 252

Query: 248 AGVIAMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFL 307
           AG IA+    ++WVT++  IRR++FEVF+YSH+ YI++++F++ HVG  +  + LPG ++
Sbjct: 253 AGEIALVAGLMMWVTTYPKIRRRLFEVFFYSHYLYIVFMLFFVFHVGISHALIPLPGFYI 312

Query: 308 FAIDRYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWH 367
           F +DR+LRFLQS+  V LVSAR LPC  VELNF K+P L YSPTS MF+N+PSISK+QWH
Sbjct: 313 FLVDRFLRFLQSRNNVKLVSARVLPCDTVELNFSKNPMLMYSPTSTMFVNIPSISKLQWH 372

Query: 368 PFTVTSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSS----VDHLQVSVEGPYGPASTHFL 427
           PFT+ S+ KLEP+ +SV+IK+ G W++KL+  LSSS    ++ L VSVEGPYGP+ST FL
Sbjct: 373 PFTIISSSKLEPETLSVMIKSQGKWSTKLYDMLSSSSSDQINRLAVSVEGPYGPSSTDFL 432

Query: 428 RHESLLLISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPV 487
           RHESL+++SGGSGITPFISI+R++  + ST   C +P++ LICAFKNS DL+MLDL+LP 
Sbjct: 433 RHESLVMVSGGSGITPFISIVRDLFYMSSTH-KCKIPKMTLICAFKNSSDLSMLDLILPT 492

Query: 488 SSTQTEISN-VPIQIEAYITREKQ---QAETNAEKLINTKWFKPNPLDSPVSEALGPDNW 547
           S   T++++ V IQI+A++TRE++   +  T+   +I T+ FKPN  D P+S  LGP++W
Sbjct: 493 SGLTTDMASFVDIQIKAFVTREEKTSVKESTHNRNIIKTRHFKPNVSDQPISPILGPNSW 552

Query: 548 LLLGAIISSSFVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSF 607
           L L AI+SSSF++F++++ IITRY+++PI++N +  Y ++YK L  +      +  +S+ 
Sbjct: 553 LCLAAILSSSFMIFIVIIAIITRYHIHPIDQNSEK-YTWAYKSLIYLVSISITVVTTSTA 612

Query: 608 VFLWERKTNASKHKQIQVLNVQSPKASPGSWIYGGERELESLPCQSLVQATNIHYGGRPD 667
             LW +K   +K+   Q ++  SP              +ES P Q + Q+T+IHYG RP+
Sbjct: 613 AMLWNKKKYYAKND--QYVDNLSPVI------------IESSPQQLISQSTDIHYGERPN 672

Query: 668 LNKILLDCKGSDIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
           LNK+L+  KGS +G+LV GP  MR  VAKICS   A+NL+F S+SF
Sbjct: 673 LNKLLVGLKGSSVGILVCGPKKMRQKVAKICSFGSAENLHFESISF 702

BLAST of Lag0020043 vs. TAIR 10
Match: AT1G23020.2 (ferric reduction oxidase 3 )

HSP 1 Score: 605.9 bits (1561), Expect = 4.1e-173
Identity = 332/700 (47.43%), Postives = 466/700 (66.57%), Query Frame = 0

Query: 7   LKMIFFLTFVLWLWLWIMLPTKLYKNTWTPELKMDL-NSTYFREQGTNLLLFSFPIMFMA 66
           +K++  +  +  + +WIM+PT  YK  W   ++  L  S YF + G NLL++ FP++ +A
Sbjct: 38  IKLLTMVILMGTVVIWIMMPTSTYKKIWLKSMRAKLGKSIYFGKPGVNLLVYMFPMILLA 97

Query: 67  VLGCFYLHLQKKSSEDPKPFSTQRSRGDSF--LRRPVLVMNSLGIVNTLEAMFAAAFVLL 126
            LG  YLHL+K++  +       R + D F  L+RP+LV   LGIV   E MF   F+ L
Sbjct: 98  SLGSIYLHLKKQTRVNQFNSRMDRKKIDKFGALKRPMLVKAGLGIVTVTEVMFLMMFMAL 157

Query: 127 LIWSLGNYLYVSFGHLHMHKV---GEKIWEAKFRSVSLRLGYIGNICWAFLFFPVTRGSS 186
           L+WSL NY Y +F  +    +   G+ +W+A+  S+++RLG  GNIC  FLF+PV RGSS
Sbjct: 158 LLWSLANYFYHTFVTITPQSLPTDGDNLWQARLDSIAVRLGLTGNICLGFLFYPVARGSS 217

Query: 187 LLPLFGLTSESTIKYHIWLGHISNFLFALHTVGFITYWAITDQMALLIEWSVDYVSNVAG 246
           LL   GLTSES+ KYHIWLG++   LF  H + +  YW  T+Q++ ++EW    +S++AG
Sbjct: 218 LLAAVGLTSESSTKYHIWLGNLVMTLFTSHGLCYCIYWISTNQVSQMLEWDRTGISHLAG 277

Query: 247 VIAMAFATVIWVTSFRPIRRKMFEVFYYSHHFYILYIIFYLIHVGAEYVCLILPGIFLFA 306
            IA+    ++W T+F  IRR+ FEVF+Y+H+ Y+++++F++ HVG  Y  +  PG ++F 
Sbjct: 278 EIALVAGLLMWATTFPAIRRRFFEVFFYTHYLYMVFMLFFVFHVGISYALISFPGFYIFM 337

Query: 307 IDRYLRFLQSQRQVSLVSARHLPCGAVELNFLKSPELSYSPTSVMFINVPSISKMQWHPF 366
           +DR+LRFLQS+  V LVSAR LPC  VELNF K+P L YSPTS++F+N+PSISK+QWHPF
Sbjct: 338 VDRFLRFLQSRNNVKLVSARVLPCETVELNFSKNPMLMYSPTSILFVNIPSISKLQWHPF 397

Query: 367 TVTSNCKLEPDIVSVVIKTGGSWTSKLHQQLSSS--VDHLQVSVEGPYGPASTHFLRHES 426
           T+TS+ KLEP  +SV+IK+ G W+SKLH  L+SS  +DHL VSVEGPYGPAST +LRH+S
Sbjct: 398 TITSSSKLEPKKLSVMIKSQGKWSSKLHHMLASSNQIDHLAVSVEGPYGPASTDYLRHDS 457

Query: 427 LLLISGGSGITPFISIIREVMIVRSTKPNCHVPQIRLICAFKNSIDLNMLDLLLPVSSTQ 486
           L+++SGGSGITPFISIIR+++ V ST      P+I LICAFKNS DL+ML+L+LP S   
Sbjct: 458 LVMVSGGSGITPFISIIRDLLYVSSTNA-YKTPKITLICAFKNSSDLSMLNLILPNS--- 517

Query: 487 TEISN-VPIQIEAYITREKQQAETNAEKLINTKWFKPNPLDSPVSEALGPDNWLLLGAII 546
           TEIS+ + IQI+A++TREK    T    +I T  FKP   D P+S  LGP++WL L  I+
Sbjct: 518 TEISSFIDIQIKAFVTREK--VSTCNMNIIKTLSFKPYVSDQPISPILGPNSWLWLATIL 577

Query: 547 SSSFVMFLILLGIITRYYVYPIERNGDALYNYSYKVLWDMFLAFACICISSSFVFLWERK 606
           SSSF++F+I++ II+RY++YPI+++    Y  +Y  L  +      +  +S+   L  +K
Sbjct: 578 SSSFMIFIIIIAIISRYHIYPIDQSSKE-YTSAYTSLIYLLAISISVVATSTVAMLCNKK 637

Query: 607 TNASKHKQI-QVLNVQSPKASPGSWIYGGERELESLPCQSLVQATNIHYGGRPDLNKILL 666
              S  K + Q ++  SP              +ES P Q L + TNIHYG RP+LNK+L+
Sbjct: 638 ---SYFKGLYQNVDALSPLM------------IESSPDQLLPEFTNIHYGERPNLNKLLV 697

Query: 667 DCKGSDIGVLVSGPTGMRHDVAKICSSAYADNLNFHSMSF 697
             KGS +GVLV GP  MR +VAKICS   A NL F S+SF
Sbjct: 698 GLKGSSVGVLVCGPRKMREEVAKICSFGSAANLQFESISF 715

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023552403.10.0e+0087.36ferric reduction oxidase 4-like [Cucurbita pepo subsp. pepo][more]
XP_038878180.10.0e+0088.59ferric reduction oxidase 4-like [Benincasa hispida][more]
XP_022922912.10.0e+0087.50ferric reduction oxidase 4-like [Cucurbita moschata][more]
KAG6576705.10.0e+0087.36Ferric reduction oxidase 4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022984974.10.0e+0086.78ferric reduction oxidase 4-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8W1101.1e-22655.78Ferric reduction oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=FRO4 PE=2 SV=1[more]
Q9FLW28.7e-22153.77Ferric reduction oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=FRO5 PE=2 SV=1[more]
P929491.6e-19049.64Ferric reduction oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=FRO2 PE=1 SV=2[more]
Q9LMM21.2e-18047.17Probable ferric reduction oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=FRO1 PE=2... [more]
F4I4K75.7e-17247.43Ferric reduction oxidase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FRO... [more]
Match NameE-valueIdentityDescription
A0A6J1E4P20.0e+0087.50ferric reduction oxidase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111430749 PE... [more]
A0A6J1JC030.0e+0086.78ferric reduction oxidase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111483082 PE=4... [more]
A0A6J1E5E90.0e+0086.93ferric reduction oxidase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111430747 PE... [more]
A0A1S3AZ420.0e+0086.68ferric reduction oxidase 4-like OS=Cucumis melo OX=3656 GN=LOC103484121 PE=4 SV=... [more]
A0A0A0L8S60.0e+0086.51FAD-binding FR-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_... [more]
Match NameE-valueIdentityDescription
AT5G23980.17.5e-22855.78ferric reduction oxidase 4 [more]
AT5G23990.18.7e-19249.29ferric reduction oxidase 5 [more]
AT1G01580.11.1e-19149.64ferric reduction oxidase 2 [more]
AT1G01590.18.2e-18247.17ferric reduction oxidase 1 [more]
AT1G23020.24.1e-17347.43ferric reduction oxidase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000778Cytochrome b245, heavy chainPRINTSPR00466GP91PHOXcoord: 614..632
score: 30.26
coord: 263..283
score: 47.62
coord: 238..261
score: 28.12
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainGENE3D3.40.50.80coord: 400..524
e-value: 2.6E-15
score: 58.8
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainGENE3D3.40.50.80coord: 593..698
e-value: 1.6E-5
score: 26.7
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainSUPERFAMILY52343Ferredoxin reductase-like, C-terminal NADP-linked domaincoord: 400..473
IPR013121Ferric reductase, NAD binding domainPFAMPF08030NAD_binding_6coord: 416..682
e-value: 2.2E-25
score: 89.6
IPR013130Ferric reductase transmembrane component-like domainPFAMPF01794Ferric_reductcoord: 161..279
e-value: 4.9E-16
score: 59.0
IPR013112FAD-binding 8PFAMPF08022FAD_binding_8coord: 313..410
e-value: 1.8E-20
score: 73.1
NoneNo IPR availableSFLDSFLDS00052Ferric_Reductase_Domaincoord: 27..696
e-value: 0.0
score: 436.3
NoneNo IPR availableSFLDSFLDG01168Ferric_reductase_subgroup_(FRcoord: 9..696
e-value: 0.0
score: 421.8
NoneNo IPR availablePANTHERPTHR11972:SF79FERRIC REDUCTION OXIDASE 4-RELATEDcoord: 3..696
NoneNo IPR availablePANTHERPTHR11972NADPH OXIDASEcoord: 3..696
NoneNo IPR availableCDDcd06186NOX_Duox_like_FAD_NADPcoord: 316..494
e-value: 5.69338E-36
score: 132.814
IPR017927FAD-binding domain, ferredoxin reductase-typePROSITEPS51384FAD_FRcoord: 309..412
score: 11.13071
IPR017938Riboflavin synthase-like beta-barrelSUPERFAMILY63380Riboflavin synthase domain-likecoord: 313..408

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0020043.1Lag0020043.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016174 NAD(P)H oxidase H2O2-forming activity
molecular_function GO:0016491 oxidoreductase activity