Lag0020032 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0020032
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionsilicon efflux transporter LSI2
Locationchr5: 47518410 .. 47520647 (-)
RNA-Seq ExpressionLag0020032
SyntenyLag0020032
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCATGGATCATACTGTAAAGGTCATTCTAGGCTCAATTGCCTTTGCAATCTTCTGGTTATTAGCTGTTTTCCCTGCTATCCCATTTCTACCAATTGGGAGAACAGCAGGATCTCTCCTAGGGGCAATGCTAATGGTCGTATTTCGAGTCCTAACTCCAGATCAAGCATATGCTGCCATTGATCTCCCAATACTTGGTCTTCTGTTTGGGACAATGGTAGTTAGTGTTTATCTTGAAAGAGCTGATATGTTCAAGTACTTGGGTAAGGTGCTGTCATGGAGGAGTAGAGGAGCCAAGGATTTAATTTGTCGAGTGTGCCTCATTTCTGCAATTTCTAGTGCTTTTTTCACCAATGACACCTCATGCGTGGTTTTGACTGAATTTGTACTAAAAATTGCAAGGCAACATAATCTTCCACCTCGTCCCTTCCTTCTTGCCCTTGCGTCAAGCGCTAATATTGGATCGTCAGCAACACCCATTGGCAATCCCCAAAACTTGGTCATAGCTGTTCAGAGTAAAATTCCTTTCGTGCAATTTGTGATTGGAATACTCCCAGCTATGCTTGTTGGTATTGTTGTAAATGCTTTAATTATTCTTATCCTATATTGGAAATTGTTATCTGTCCAAAAGGATGAAGAAGATGTATCCCCAGAAATTATTGCAGATGAAGATGTGAATTCTCATAGGTTTTCACCAGCCAGATTGTCACACTCTCAAATTCCATCCATCAACTCTCTGGAATGGAATTCTCGATTGGAGCTAATGAACGCACAAGGTTCTCCTTGCTCGAATGCACATGTTGAAAATGTTGAAACTCTAAGAAATTCAGTAAGTTCAAAAGATAATGAAATCCACAGGTCTCATAGTGCTATGACGGAGTCGGCAAGAATATCTGATGCATCAAAAGAGTGGGTGGCCATTGCATCTCCTCAGAAAAGGGAAGAAGGTCTTTCTTCTAAGAGCCTCAACTCTATGGATAAACAGAAAGAAGCAGTATCCTTGCAGTTGCAGTCCTCAGAAGGAAAGGAACATTGGAGCACAAAATGGAGAAAGATTGCATGGAAAAGTTGTGTTTATCTTGTGACTTTAGGAATGCTTATTGCTTTATTGATGGGCTTGAATATGTCATGGACTGCAGTGACTGCTGCACTCGCTCTTGTGGTTCTTGATTTCAAGGATGCTCAGCCATGCCTAGAAAAGGTTTGAACCTTCATTTCTTTGTGCATCTTTAATTACTACCAAATATAATTTCTCGATTGAATTTTTGTATGGCGAATGATAAGAACTTGTTTTCTATTTCTACAGGTTTCCTATTCACTTTTAGTCTTCTTTTGTGGGATGTTTATGACGGTCGATGGATTTAACAAGACTGGATTACCAAGTGCTTTTTGGAATTTCATGGAGCCGCATGCGCAGATTGATCGTGTTTCTGGGACTGTAATTCTTGCTCTTGTCATACTATACCTCTCAAACTTGGCTTCAAACGTGCCTACTGGTATTTAAATACTTATCATTCCATTGTTCCTTGTTTTCCAATTTTCCAATTTTTCTTTTTCCAGTTATATCTTCTGTATGTTATAGCCTTTATATAGAATGAAAATTCTAGTATAGCAAAGAAACCCTGAACCTCATTTGGAGGATTTTTGGTGTCTGGTTATATGAAATGCACAAAATAATATGTCAAGATTCAAGAATTATCTGATTAGAGGACAATCACTGCAAATGATGTGTATAAATAAGCTTGAGGTTATGCATGTATTGACCAAATCACAAATTCATTTTCATGGAAACAACTGAGGTTTCGATATTATTTGAACAATATTTTGAATGAAAAGTGATAACCACATCATTTTTCTTGGATTAGTGAACATTATGTTATTCACTAGCTCGATTGACCTGTTAGTTGTCATGAAAATCTAGCTAATCTGCAACTGCAATTGTGTCTAATGCTTTTTCATTGTCATCTTGTCAGTTCTTCTGCTCGGAGCTCGGGTCGCCGCATCTGCTGCTGCAATATCTGCAGCTGAAGAAAAACGGGCATGGCTTCTTTTAGCTTGGATCAGTACTGTAGCTGGAAATCTCTCATTGTTAGGCTCAGCTGCCAACTTGATTGTGTGCGAACAGGCTCGTCGCAGTCCACAGCTAAGCTACAATTTATCCTTCTGGAATCATCTTAAATTTGGACTTCCTTCAACTCTTATTGTCACAGCTATTGGTTTGGTTCTCATAAGATGA

mRNA sequence

ATGGCCATGGATCATACTGTAAAGGTCATTCTAGGCTCAATTGCCTTTGCAATCTTCTGGTTATTAGCTGTTTTCCCTGCTATCCCATTTCTACCAATTGGGAGAACAGCAGGATCTCTCCTAGGGGCAATGCTAATGGTCGTATTTCGAGTCCTAACTCCAGATCAAGCATATGCTGCCATTGATCTCCCAATACTTGGTCTTCTGTTTGGGACAATGGTAGTTAGTGTTTATCTTGAAAGAGCTGATATGTTCAAGTACTTGGGTAAGGTGCTGTCATGGAGGAGTAGAGGAGCCAAGGATTTAATTTGTCGAGTGTGCCTCATTTCTGCAATTTCTAGTGCTTTTTTCACCAATGACACCTCATGCGTGGTTTTGACTGAATTTGTACTAAAAATTGCAAGGCAACATAATCTTCCACCTCGTCCCTTCCTTCTTGCCCTTGCGTCAAGCGCTAATATTGGATCGTCAGCAACACCCATTGGCAATCCCCAAAACTTGGTCATAGCTGTTCAGAGTAAAATTCCTTTCGTGCAATTTGTGATTGGAATACTCCCAGCTATGCTTGTTGGTATTGTTGTAAATGCTTTAATTATTCTTATCCTATATTGGAAATTGTTATCTGTCCAAAAGGATGAAGAAGATGTATCCCCAGAAATTATTGCAGATGAAGATGTGAATTCTCATAGGTTTTCACCAGCCAGATTGTCACACTCTCAAATTCCATCCATCAACTCTCTGGAATGGAATTCTCGATTGGAGCTAATGAACGCACAAGGTTCTCCTTGCTCGAATGCACATGTTGAAAATGTTGAAACTCTAAGAAATTCAGTAAGTTCAAAAGATAATGAAATCCACAGGTCTCATAGTGCTATGACGGAGTCGGCAAGAATATCTGATGCATCAAAAGAGTGGGTGGCCATTGCATCTCCTCAGAAAAGGGAAGAAGGTCTTTCTTCTAAGAGCCTCAACTCTATGGATAAACAGAAAGAAGCAGTATCCTTGCAGTTGCAGTCCTCAGAAGGAAAGGAACATTGGAGCACAAAATGGAGAAAGATTGCATGGAAAAGTTGTGTTTATCTTGTGACTTTAGGAATGCTTATTGCTTTATTGATGGGCTTGAATATGTCATGGACTGCAGTGACTGCTGCACTCGCTCTTGTGGTTCTTGATTTCAAGGATGCTCAGCCATGCCTAGAAAAGGTTTCCTATTCACTTTTAGTCTTCTTTTGTGGGATGTTTATGACGGTCGATGGATTTAACAAGACTGGATTACCAAGTGCTTTTTGGAATTTCATGGAGCCGCATGCGCAGATTGATCGTGTTTCTGGGACTGTAATTCTTGCTCTTGTCATACTATACCTCTCAAACTTGGCTTCAAACGTGCCTACTGTTCTTCTGCTCGGAGCTCGGGTCGCCGCATCTGCTGCTGCAATATCTGCAGCTGAAGAAAAACGGGCATGGCTTCTTTTAGCTTGGATCAGTACTGTAGCTGGAAATCTCTCATTGTTAGGCTCAGCTGCCAACTTGATTGTGTGCGAACAGGCTCGTCGCAGTCCACAGCTAAGCTACAATTTATCCTTCTGGAATCATCTTAAATTTGGACTTCCTTCAACTCTTATTGTCACAGCTATTGGTTTGGTTCTCATAAGATGA

Coding sequence (CDS)

ATGGCCATGGATCATACTGTAAAGGTCATTCTAGGCTCAATTGCCTTTGCAATCTTCTGGTTATTAGCTGTTTTCCCTGCTATCCCATTTCTACCAATTGGGAGAACAGCAGGATCTCTCCTAGGGGCAATGCTAATGGTCGTATTTCGAGTCCTAACTCCAGATCAAGCATATGCTGCCATTGATCTCCCAATACTTGGTCTTCTGTTTGGGACAATGGTAGTTAGTGTTTATCTTGAAAGAGCTGATATGTTCAAGTACTTGGGTAAGGTGCTGTCATGGAGGAGTAGAGGAGCCAAGGATTTAATTTGTCGAGTGTGCCTCATTTCTGCAATTTCTAGTGCTTTTTTCACCAATGACACCTCATGCGTGGTTTTGACTGAATTTGTACTAAAAATTGCAAGGCAACATAATCTTCCACCTCGTCCCTTCCTTCTTGCCCTTGCGTCAAGCGCTAATATTGGATCGTCAGCAACACCCATTGGCAATCCCCAAAACTTGGTCATAGCTGTTCAGAGTAAAATTCCTTTCGTGCAATTTGTGATTGGAATACTCCCAGCTATGCTTGTTGGTATTGTTGTAAATGCTTTAATTATTCTTATCCTATATTGGAAATTGTTATCTGTCCAAAAGGATGAAGAAGATGTATCCCCAGAAATTATTGCAGATGAAGATGTGAATTCTCATAGGTTTTCACCAGCCAGATTGTCACACTCTCAAATTCCATCCATCAACTCTCTGGAATGGAATTCTCGATTGGAGCTAATGAACGCACAAGGTTCTCCTTGCTCGAATGCACATGTTGAAAATGTTGAAACTCTAAGAAATTCAGTAAGTTCAAAAGATAATGAAATCCACAGGTCTCATAGTGCTATGACGGAGTCGGCAAGAATATCTGATGCATCAAAAGAGTGGGTGGCCATTGCATCTCCTCAGAAAAGGGAAGAAGGTCTTTCTTCTAAGAGCCTCAACTCTATGGATAAACAGAAAGAAGCAGTATCCTTGCAGTTGCAGTCCTCAGAAGGAAAGGAACATTGGAGCACAAAATGGAGAAAGATTGCATGGAAAAGTTGTGTTTATCTTGTGACTTTAGGAATGCTTATTGCTTTATTGATGGGCTTGAATATGTCATGGACTGCAGTGACTGCTGCACTCGCTCTTGTGGTTCTTGATTTCAAGGATGCTCAGCCATGCCTAGAAAAGGTTTCCTATTCACTTTTAGTCTTCTTTTGTGGGATGTTTATGACGGTCGATGGATTTAACAAGACTGGATTACCAAGTGCTTTTTGGAATTTCATGGAGCCGCATGCGCAGATTGATCGTGTTTCTGGGACTGTAATTCTTGCTCTTGTCATACTATACCTCTCAAACTTGGCTTCAAACGTGCCTACTGTTCTTCTGCTCGGAGCTCGGGTCGCCGCATCTGCTGCTGCAATATCTGCAGCTGAAGAAAAACGGGCATGGCTTCTTTTAGCTTGGATCAGTACTGTAGCTGGAAATCTCTCATTGTTAGGCTCAGCTGCCAACTTGATTGTGTGCGAACAGGCTCGTCGCAGTCCACAGCTAAGCTACAATTTATCCTTCTGGAATCATCTTAAATTTGGACTTCCTTCAACTCTTATTGTCACAGCTATTGGTTTGGTTCTCATAAGATGA

Protein sequence

MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAAIDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWRSRGAKDLICRVCLISAISSAFFTNDTSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFVQFVIGILPAMLVGIVVNALIILILYWKLLSVQKDEEDVSPEIIADEDVNSHRFSPARLSHSQIPSINSLEWNSRLELMNAQGSPCSNAHVENVETLRNSVSSKDNEIHRSHSAMTESARISDASKEWVAIASPQKREEGLSSKSLNSMDKQKEAVSLQLQSSEGKEHWSTKWRKIAWKSCVYLVTLGMLIALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTGLPSAFWNFMEPHAQIDRVSGTVILALVILYLSNLASNVPTVLLLGARVAASAAAISAAEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFGLPSTLIVTAIGLVLIR
Homology
BLAST of Lag0020032 vs. NCBI nr
Match: XP_038903236.1 (silicon efflux transporter LSI2 [Benincasa hispida] >XP_038903243.1 silicon efflux transporter LSI2 [Benincasa hispida] >XP_038903252.1 silicon efflux transporter LSI2 [Benincasa hispida])

HSP 1 Score: 975.3 bits (2520), Expect = 2.2e-280
Identity = 518/551 (94.01%), Postives = 535/551 (97.10%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAMDHTVKVILGSIAFA+FWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWRSRGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSW+S+GAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFVQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPF QF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180

Query: 181 VIGILPAMLVGIVVNALIILILYWKLLSVQKDEEDVSPEIIADEDVNSHRFSPARLSHSQ 240
           VIGILPAMLVG+VVNALIILILYWKLLSVQKDEED SPEI+ADEDV SHRFSPARLSH+Q
Sbjct: 181 VIGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEIVADEDVLSHRFSPARLSHTQ 240

Query: 241 IPSINSLEWNSRLELMNAQGSPCSNAHVENVETLRNSVSSKDNEIHRSHSAMTESARISD 300
           IPSINS EW+SRL+LMNAQ SPCSNA   NVETLRNSVSSKDNEIHRSHSAMTESARISD
Sbjct: 241 IPSINSAEWDSRLDLMNAQSSPCSNA---NVETLRNSVSSKDNEIHRSHSAMTESARISD 300

Query: 301 ASKEWVAIASPQKREEGLSSKSLNSMDKQKEAVSLQLQSSEGKEHWSTKWRKIAWKSCVY 360
           ASKEW+  ASPQKREEG SSKS+NSM+KQKE VS  LQSSEGKEHWSTKWR+IAWK CVY
Sbjct: 301 ASKEWLPNASPQKREEGFSSKSVNSMEKQKEPVS--LQSSEGKEHWSTKWRRIAWKCCVY 360

Query: 361 LVTLGMLIALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF 420
           LVT+GMLIALL+GLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF
Sbjct: 361 LVTIGMLIALLLGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF 420

Query: 421 NKTGLPSAFWNFMEPHAQIDRVSGTVILALVILYLSNLASNVPTVLLLGARVAASAAAIS 480
           NKTGLPSAFWNFMEPHAQIDRVSGTV+LALVILYLSNLASNVPTVLLLGARVAASAAAIS
Sbjct: 421 NKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAIS 480

Query: 481 AAEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFGLPSTL 540
           A EEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARR+PQLSYNLSFWNHLKFG+PSTL
Sbjct: 481 ATEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTL 540

Query: 541 IVTAIGLVLIR 552
           IVTAIGLVLIR
Sbjct: 541 IVTAIGLVLIR 546

BLAST of Lag0020032 vs. NCBI nr
Match: XP_022141316.1 (silicon efflux transporter LSI2 [Momordica charantia] >XP_022141318.1 silicon efflux transporter LSI2 [Momordica charantia] >XP_022141319.1 silicon efflux transporter LSI2 [Momordica charantia] >XP_022141320.1 silicon efflux transporter LSI2 [Momordica charantia])

HSP 1 Score: 970.7 bits (2508), Expect = 5.3e-279
Identity = 506/551 (91.83%), Postives = 531/551 (96.37%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMV+FRVLTPDQAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVIFRVLTPDQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWRSRGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSW+SRGAKDLICRVC+ISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSRGAKDLICRVCVISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFVQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPF QF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180

Query: 181 VIGILPAMLVGIVVNALIILILYWKLLSVQKDEEDVSPEIIADEDVNSHRFSPARLSHSQ 240
           VIGILPAMLVGIVVNA+IIL+LYWKLLSVQKDEED   E++ADEDVNSHRFSPARLSH+ 
Sbjct: 181 VIGILPAMLVGIVVNAVIILVLYWKLLSVQKDEEDAPSEVVADEDVNSHRFSPARLSHTH 240

Query: 241 IPSINSLEWNSRLELMNAQGSPCSNAHVENVETLRNSVSSKDNEIHRSHSAMTESARISD 300
           IPSINS EWNSRL+ MNAQ SPC+NA++ENVET+RNS+SSKDNEIHRS SAMTESAR+SD
Sbjct: 241 IPSINSPEWNSRLDSMNAQSSPCANANIENVETVRNSISSKDNEIHRSLSAMTESARVSD 300

Query: 301 ASKEWVAIASPQKREEGLSSKSLNSMDKQKEAVSLQLQSSEGKEHWSTKWRKIAWKSCVY 360
           ASKEW+  +SPQKREE   SKS NSMDKQKE VS+ LQSSEGKEHWSTKWR+IAWK CVY
Sbjct: 301 ASKEWLPTSSPQKREESFPSKSFNSMDKQKELVSVSLQSSEGKEHWSTKWRRIAWKCCVY 360

Query: 361 LVTLGMLIALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF 420
           LVT+GML+ALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF
Sbjct: 361 LVTIGMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF 420

Query: 421 NKTGLPSAFWNFMEPHAQIDRVSGTVILALVILYLSNLASNVPTVLLLGARVAASAAAIS 480
           NKTGLPSAFWNFMEPHAQIDRVSGTV+LALVILYLSNLASNVPTVLLLGARVAASAAAIS
Sbjct: 421 NKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAIS 480

Query: 481 AAEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFGLPSTL 540
             +EKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFG+PSTL
Sbjct: 481 PDQEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFGVPSTL 540

Query: 541 IVTAIGLVLIR 552
           +VTAIGLVLIR
Sbjct: 541 LVTAIGLVLIR 551

BLAST of Lag0020032 vs. NCBI nr
Match: XP_016899111.1 (PREDICTED: putative transporter arsB [Cucumis melo] >XP_016899112.1 PREDICTED: putative transporter arsB [Cucumis melo] >XP_016899113.1 PREDICTED: putative transporter arsB [Cucumis melo] >XP_016899114.1 PREDICTED: putative transporter arsB [Cucumis melo] >KAA0033676.1 putative transporter arsB [Cucumis melo var. makuwa] >TYK22974.1 putative transporter arsB [Cucumis melo var. makuwa])

HSP 1 Score: 964.1 bits (2491), Expect = 5.0e-277
Identity = 511/551 (92.74%), Postives = 531/551 (96.37%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAMDHTVKVILGSIAFA+FWLLAVFPAIPFLPIGRTAGS+LGAMLMV+FRVLTP+QAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVIFRVLTPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWRSRGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSW+S+GAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFVQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPF QF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180

Query: 181 VIGILPAMLVGIVVNALIILILYWKLLSVQKDEEDVSPEIIADEDVNSHRFSPARLSHSQ 240
           VIGILPAMLVG+VVNALIILILYWKLLSVQKDEED SPE+IADEDV SHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240

Query: 241 IPSINSLEWNSRLELMNAQGSPCSNAHVENVETLRNSVSSKDNEIHRSHSAMTESARISD 300
           IPS+NS EW+SRL+ MNAQ  PCSNA   NVET+RNSVSSKDNEIHRSHS +TE ARISD
Sbjct: 241 IPSLNSAEWDSRLDSMNAQSPPCSNA---NVETIRNSVSSKDNEIHRSHSTLTEPARISD 300

Query: 301 ASKEWVAIASPQKREEGLSSKSLNSMDKQKEAVSLQLQSSEGKEHWSTKWRKIAWKSCVY 360
           ASKEW+  ASPQKREEG SSKSLNSMDKQKE V   LQSSEGKEHWSTKWR+IAWKSCVY
Sbjct: 301 ASKEWLPNASPQKREEGFSSKSLNSMDKQKEPVI--LQSSEGKEHWSTKWRRIAWKSCVY 360

Query: 361 LVTLGMLIALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF 420
           LVT+GMLIALL+GL+MSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF
Sbjct: 361 LVTVGMLIALLLGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF 420

Query: 421 NKTGLPSAFWNFMEPHAQIDRVSGTVILALVILYLSNLASNVPTVLLLGARVAASAAAIS 480
           NKTGLPSAFWNFMEPHAQIDRVSGTV+LALVILYLSNLASNVPTVLLLGARVAASAAAIS
Sbjct: 421 NKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAIS 480

Query: 481 AAEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFGLPSTL 540
            AEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARR+PQLSYNLSFWNHLKFGLPSTL
Sbjct: 481 PAEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTL 540

Query: 541 IVTAIGLVLIR 552
           IVTAIGLVLIR
Sbjct: 541 IVTAIGLVLIR 546

BLAST of Lag0020032 vs. NCBI nr
Match: XP_004140769.1 (silicon efflux transporter LSI3 [Cucumis sativus] >XP_011651172.1 silicon efflux transporter LSI3 [Cucumis sativus] >XP_011651173.1 silicon efflux transporter LSI3 [Cucumis sativus] >XP_031738919.1 silicon efflux transporter LSI3 [Cucumis sativus] >APT69295.1 LSi2 [Cucumis sativus var. sativus] >KGN57393.1 hypothetical protein Csa_011781 [Cucumis sativus])

HSP 1 Score: 945.3 bits (2442), Expect = 2.4e-271
Identity = 505/552 (91.49%), Postives = 526/552 (95.29%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAMDHTVKVILGSIAFA+FWLLAVFPAIPFLPIGRTAGS+LGAMLMVVFRVLTP+QAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWRSRGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSW+S+GAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFVQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKI F QF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIHFGQF 180

Query: 181 VIGILPAMLVGIVVNALIILILYWKLLSVQKDEEDVSPEIIADEDVNSHRFSPARLSHSQ 240
           VIGILPAMLVG+VVNALIILI+YWKLLSVQKDEED SPE+IADEDV SHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240

Query: 241 IPSINSLEWNSRLELMNAQGSPCSNAHVENVETLRNSVSSKDN-EIHRSHSAMTESARIS 300
           IPS+NS EW+SRL+LMNAQ  PCSN    NVET+RNSVSSKDN EI RSHS MTE ARIS
Sbjct: 241 IPSLNSAEWDSRLDLMNAQSPPCSNT---NVETIRNSVSSKDNEEIRRSHSTMTEPARIS 300

Query: 301 DASKEWVAIASPQKREEGLSSKSLNSMDKQKEAVSLQLQSSEGKEHWSTKWRKIAWKSCV 360
           DASKEW+  AS QKREE  SSKSLNSM+KQKE V   LQSSEGKEHWSTKWR+IAWKSCV
Sbjct: 301 DASKEWLPNASTQKREEDFSSKSLNSMEKQKEPVI--LQSSEGKEHWSTKWRRIAWKSCV 360

Query: 361 YLVTLGMLIALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDG 420
           YLVT+GML+ALLMGL+MSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDG
Sbjct: 361 YLVTVGMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDG 420

Query: 421 FNKTGLPSAFWNFMEPHAQIDRVSGTVILALVILYLSNLASNVPTVLLLGARVAASAAAI 480
           FNKTGLPSAFWNFMEPHAQIDRVSGTV+LALVILYLSNLASNVPTVLLLGARVAASAAAI
Sbjct: 421 FNKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAI 480

Query: 481 SAAEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFGLPST 540
           S  EEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARR+PQLSYNLSFWNHLKFGLPST
Sbjct: 481 SPTEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPST 540

Query: 541 LIVTAIGLVLIR 552
           LIVTAIGLVLI+
Sbjct: 541 LIVTAIGLVLIK 547

BLAST of Lag0020032 vs. NCBI nr
Match: KAG7013248.1 (putative transporter arsB, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 922.2 bits (2382), Expect = 2.2e-264
Identity = 497/554 (89.71%), Postives = 520/554 (93.86%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAMDHTVKVILGSIAF IFWLLAVFPAIPFLPIGRTAGSLLGAMLMV+FRVLTPDQAYAA
Sbjct: 101 MAMDHTVKVILGSIAFGIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVLFRVLTPDQAYAA 160

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWRSRGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSW+S+GAKDLICRVCLISAISSA FTND
Sbjct: 161 IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSALFTND 220

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFVQF 180
           TSCVVLTEFVLKIA+QHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQS IPF QF
Sbjct: 221 TSCVVLTEFVLKIAKQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSGIPFGQF 280

Query: 181 VIGILPAMLVGIVVNALIILILYWKLLSVQKDEEDVSPEIIADEDVNSHRFSPARLSHSQ 240
           VIGILPAMLVGIVVNALIILI YWKLLS+QKDEEDV PEI+ADEDVNSHRFSPARLSHSQ
Sbjct: 281 VIGILPAMLVGIVVNALIILISYWKLLSIQKDEEDVPPEIVADEDVNSHRFSPARLSHSQ 340

Query: 241 IPSINSLEWNSRLELMNAQGSPCSNAHVENVETLRNSVSSKDNEIHRSHSAMTES-ARIS 300
           IPS+NS EW+SRL+ M AQ  P SNA   NVETLR SVSSKDNEIH SH A+TE+ ARIS
Sbjct: 341 IPSLNSQEWDSRLDSMYAQSPPNSNA---NVETLRTSVSSKDNEIHWSHGAVTEAVARIS 400

Query: 301 DASKEWVAIAS--PQKREEGLSSKSLNSMDKQKEAVSLQLQSSEGKEHWSTKWRKIAWKS 360
           DASKE V  AS  PQKREEGLSSKS NSMDKQKE   L LQ SEGK+HWSTKWR++AWK 
Sbjct: 401 DASKECVPNASPPPQKREEGLSSKSFNSMDKQKE--PLPLQVSEGKQHWSTKWRRMAWKC 460

Query: 361 CVYLVTLGMLIALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTV 420
           CVYLVT+GML+ALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMF+TV
Sbjct: 461 CVYLVTIGMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFITV 520

Query: 421 DGFNKTGLPSAFWNFMEPHAQIDRVSGTVILALVILYLSNLASNVPTVLLLGARVAASAA 480
           DGFNKTGLP+ FWNFMEPHAQIDRVSGTV+LALVILYLSNLASNVPTVLLLGARVAASAA
Sbjct: 521 DGFNKTGLPNTFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAA 580

Query: 481 AISAAEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFGLP 540
           AIS A+EKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARR+PQLSYNLSFWNHLKFGLP
Sbjct: 581 AISPAQEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLP 640

Query: 541 STLIVTAIGLVLIR 552
           STLI+T IGLVLI+
Sbjct: 641 STLIITGIGLVLIK 649

BLAST of Lag0020032 vs. ExPASy Swiss-Prot
Match: Q9AV23 (Silicon efflux transporter LSI3 OS=Oryza sativa subsp. japonica OX=39947 GN=LSI3 PE=2 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 4.5e-148
Identity = 298/554 (53.79%), Postives = 387/554 (69.86%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MA+    KV++GS+AF +FW+LAVFP++PFLPIGRTAG+LLGA+LM+VF V++ D AYA+
Sbjct: 1   MALASLPKVVMGSVAFGVFWMLAVFPSVPFLPIGRTAGALLGAVLMIVFHVISADDAYAS 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWRSRGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLF TMVV  YL+ A MF++LG++L+WRS+G +DL+CRVC+++A++SA FTND
Sbjct: 61  IDLPILGLLFATMVVGGYLKNAGMFRHLGRLLAWRSQGGRDLMCRVCVVTALASALFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFVQF 180
           T CVVLTEFVL++A + NLP +PFLLALA+SANIGSSATPIGNPQNLVIA  SKI F+ F
Sbjct: 121 TCCVVLTEFVLELAAERNLPAKPFLLALATSANIGSSATPIGNPQNLVIAFNSKISFISF 180

Query: 181 VIGILPAMLVGIVVNALIILILYWKLLSVQKDEEDVSPEIIADEDVNSHRFSPARLSHSQ 240
           ++GILPAML G+ +N L++L +YWK L    D    SP+ +A                  
Sbjct: 181 LLGILPAMLAGMGINMLMLLCMYWKEL----DGGACSPDEVAAG---------------- 240

Query: 241 IPSINSLEWNSRLELMNAQGSPCSNAHVENVETLRNSVSSKDNEIHRSHSAMTESARISD 300
              + ++E   R  L N                     + KD+                D
Sbjct: 241 -KQMEAIEEGRRTALNN---------------------NKKDD---------------GD 300

Query: 301 ASKEWVAIASPQKREEGLSSKSLNSMDKQKEAVSLQLQSSEGKEHW----STKWRKIAWK 360
           A+      ASP+  + G             +A S+  ++   K  W    S   RK+  K
Sbjct: 301 AATP----ASPEDDDGG-------------DAESMMSENISTKHRWFMQCSEHRRKLFLK 360

Query: 361 SCVYLVTLGMLIALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMT 420
           S  Y+VT+GML+A ++GLNMSWTA+T A+ALVV+DF+DA+PCL+KVSYSLLVFF GMF+T
Sbjct: 361 SFAYVVTVGMLVAYMLGLNMSWTAITTAIALVVVDFRDAEPCLDKVSYSLLVFFSGMFVT 420

Query: 421 VDGFNKTGLPSAFWNFMEPHAQIDRVSGTVILALVILYLSNLASNVPTVLLLGARVAASA 480
           V GFNKTGLP A WN M P+++I+ V+G  +L+++IL LSNLASNVPTVLL+G  VAA+A
Sbjct: 421 VSGFNKTGLPGAIWNVMAPYSKINHVTGVTVLSVIILLLSNLASNVPTVLLMGDEVAAAA 480

Query: 481 AAISAAEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFGL 540
           A IS A   R+WLLLAW+STVAGNLSLLGSAANLIVCEQARR+ + +Y+L+FWNH+ FGL
Sbjct: 481 ATISPAAVTRSWLLLAWVSTVAGNLSLLGSAANLIVCEQARRATRNAYDLTFWNHVIFGL 480

Query: 541 PSTLIVTAIGLVLI 551
           PSTL+VTAIG+ LI
Sbjct: 541 PSTLVVTAIGIPLI 480

BLAST of Lag0020032 vs. ExPASy Swiss-Prot
Match: Q10SY9 (Silicon efflux transporter LSI2 OS=Oryza sativa subsp. japonica OX=39947 GN=LSI2 PE=1 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 1.5e-143
Identity = 287/543 (52.85%), Postives = 370/543 (68.14%), Query Frame = 0

Query: 8   KVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAAIDLPILG 67
           KV LGSIAFA+FW++AVFP++PFLPIGRTAGSLL A+LMV+F V++PD AYA+IDLPILG
Sbjct: 9   KVALGSIAFAVFWMMAVFPSVPFLPIGRTAGSLLSAVLMVIFHVISPDDAYASIDLPILG 68

Query: 68  LLFGTMVVSVYLERADMFKYLGKVLSWRSRGAKDLICRVCLISAISSAFFTNDTSCVVLT 127
           LLF TMVV  YL  A MFK+LG++L+W+S+G +DL+CRVC+++A++SA FTNDT CVVLT
Sbjct: 69  LLFATMVVGSYLRNAGMFKHLGRLLAWKSQGGRDLMCRVCVVTALASALFTNDTCCVVLT 128

Query: 128 EFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFVQFVIGILPA 187
           EFVL++A + NLP +PFLLALASSANIGS+ATPIGNPQNLVIA  SKI F +F++GILPA
Sbjct: 129 EFVLELAAERNLPAKPFLLALASSANIGSAATPIGNPQNLVIAFNSKITFPKFLMGILPA 188

Query: 188 MLVGIVVNALIILILYWKLLSVQKDEEDVSPEIIADEDVNSHRFSPARLSHSQIPSINSL 247
           MLVG+ VN +++L +YW+                                          
Sbjct: 189 MLVGMAVNMVMLLCMYWR------------------------------------------ 248

Query: 248 EWNSRLELMNAQGSPCSNAHVENVETLRNSVSSKDNEIHRSHSAMTESARISDASKEWVA 307
                 EL            +E VE  R+  S+K                          
Sbjct: 249 ------ELGGGAELSVDGKQMEAVEEGRSPASAK-------------------------- 308

Query: 308 IASPQKREEGLSSKSLNSMDKQKEAVSLQLQSSEGKEHWSTKWRKIAWKSCVYLVTLGML 367
            ++PQ    G +  SL   +         +Q +E +       RK+  KS  Y+VT+GM+
Sbjct: 309 -STPQLNGNGNTMMSLEMSENITTKHPWFMQCTEAR-------RKLFLKSFAYVVTVGMV 368

Query: 368 IALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTGLPS 427
           +A ++GLNMSWTA+T ALALVV+DF+DA+PCL+ VSYSLLVFF GMF+TV GFNKTGLP 
Sbjct: 369 VAYMVGLNMSWTAITTALALVVVDFRDAEPCLDTVSYSLLVFFSGMFITVSGFNKTGLPG 428

Query: 428 AFWNFMEPHAQIDRVSGTVILALVILYLSNLASNVPTVLLLGARVAASAAAISAAEEKRA 487
           A W+FM P+++++ V G  +L+++IL LSNLASNVPTVLL+G  VA +AA IS A    +
Sbjct: 429 AIWDFMAPYSKVNSVGGISVLSVIILLLSNLASNVPTVLLMGDEVAKAAALISPAAVTTS 469

Query: 488 WLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFGLPSTLIVTAIGL 547
           WLLLAW+STVAGNLSLLGSAANLIVCEQARR+P+ +Y+L+FW H+ FG+PSTLIVTA+G+
Sbjct: 489 WLLLAWVSTVAGNLSLLGSAANLIVCEQARRAPRNAYDLTFWQHIVFGVPSTLIVTAVGI 469

Query: 548 VLI 551
            LI
Sbjct: 549 PLI 469

BLAST of Lag0020032 vs. ExPASy Swiss-Prot
Match: Q54GU0 (Putative transporter arsB OS=Dictyostelium discoideum OX=44689 GN=arsB PE=2 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 6.3e-57
Identity = 167/534 (31.27%), Postives = 278/534 (52.06%), Query Frame = 0

Query: 31  LPIGRTAGSLLGAMLMVVFRVLTPDQAYAAIDLPILGLLFGTMVVSVYLERADMFKYLGK 90
           LPIGR   S++GA LMV F ++ P +  + I+   + LL   M++S Y+E+A+++    K
Sbjct: 66  LPIGRAGSSIIGATLMVYFGIIQPKEIGSVINWDTIILLMSMMMLSNYMEQANIWGMASK 125

Query: 91  VLSWRSRGAKDLICRVCLISAISSAFFTNDTSCVVLTEFVLKIARQHNLPPRPFLLALAS 150
           +L W+ +     + RVCLIS+I S+  TNDT CV LT  V+   +  NL   PFL+A+A+
Sbjct: 126 ILLWKCKSTSIFMVRVCLISSIMSSILTNDTVCVTLTPIVISACKSTNLTFFPFLMAIAT 185

Query: 151 SANIGSSATPIGNPQNLVIAVQSKIPFVQFVIGILPAMLVGIVVNALIILILYWKLLSVQ 210
           SANIGSSA P+GNPQN++IA    + F  F    + + ++G+ +N +++L+ + K L   
Sbjct: 186 SANIGSSALPVGNPQNMIIATAGGLNFFNFFKVSIVSSILGVCLNTILLLLYFKKDLKNL 245

Query: 211 KDE-----EDVSPEIIADEDVNSHRFSPARLSHSQIPSINSLEWNSRLELMNAQGSPCSN 270
                   E V+P++   E++++        +H      N+   N + E+ N        
Sbjct: 246 NSNFNQLIETVNPKV---EEIDN--------NHHDDDGANNQSKNEK-EMENINKEVEEE 305

Query: 271 AHVENVETLRNSVSSKDNEIHRSHSAM-----TESARISDASKEWVAIASPQKREEGLSS 330
            H  + +       +K+N  +  H+ +      +S  +SD S     I   +K++E    
Sbjct: 306 QHNNDDDDDDGFNENKNNNNNGGHAILLVASSMDSIDLSDCS----IINKDKKKKENFIE 365

Query: 331 KSLNSMDKQKEAVSLQLQSSEGKEHWSTKWRKIAWKSCVYLVTLGMLIALLMGLNMSWTA 390
              NS +K  E +                  K+ +K  V ++   +LI   +G++M +T 
Sbjct: 366 IYFNSKEKSIETI--------------VNIIKLIFKFRVAIILTLVLIGFFIGMHMGFTV 425

Query: 391 VTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTGLPSAFWNFMEPHAQID 450
           +     L++ + KD    +  V + LL+FF G+F+ V+GF++     A W  +EP   ID
Sbjct: 426 LFGVSILMICERKDITDIINSVDWELLLFFSGLFVLVEGFDRQFEKEA-WTILEPFVPID 485

Query: 451 ----RVSGTVILALVILYLSNLASNVPTVLLLGARVAASAAAISAAEEKRAWLLLAWIST 510
                V    I +++IL L N+  NVP VL L  R+      + A      W+LLA++ST
Sbjct: 486 STHLNVLKIFIFSILILVLCNILGNVPLVLSLSPRL------LEALAPDFTWILLAFVST 545

Query: 511 VAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFGLPSTLIVTAIGLVLI 551
           VAGNL+L+GS ANLIV E+++      + + F  +LKFG+PST++V  IG+ ++
Sbjct: 546 VAGNLTLVGSVANLIVAEKSKS----YHEIGFLEYLKFGVPSTILVILIGVPIV 558

BLAST of Lag0020032 vs. ExPASy Swiss-Prot
Match: Q62052 (P protein OS=Mus musculus OX=10090 GN=Oca2 PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 4.6e-15
Identity = 124/562 (22.06%), Postives = 229/562 (40.75%), Query Frame = 0

Query: 14  IAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA-----IDLPILGL 73
           +A A+  L  V+  I F  + RT  ++LGA+  +    +  D+         ID   L L
Sbjct: 326 VASAVAILAGVYTLIIFEIVHRTLAAMLGALAALAALAVVGDRPSLTHVVEWIDFETLAL 385

Query: 74  LFGTMVVSVYLERADMFKYLGKVLSWRSRG-AKDLICRVCLISAISSAFFTNDTSCVVLT 133
           LFG M++         F Y        SRG    +I  +CL++AI SAF  N T+ ++ T
Sbjct: 386 LFGMMILVAVFSETGFFDYCAVKAYQLSRGRVWAMIFMLCLMAAILSAFLDNVTTMLLFT 445

Query: 134 EFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSK-----IPFVQFVI 193
              +++    NL PR  L+A     NIG +AT IG+P N++I    +     + F  F  
Sbjct: 446 PVTIRLCEVLNLDPRQVLIAEVIFTNIGGAATAIGDPPNVIIVSNQELRKMGLDFAGFTA 505

Query: 194 GILPAMLVGIVVNALIILILYWKLLSVQKDEEDVSPEIIADEDVNSHRFSPARLSHSQIP 253
            +   + + ++V+  ++ +LYW      K+  ++   +    +++  R +  R+S +   
Sbjct: 506 HMFLGICLVLLVSFPLLRLLYWNKKLYNKEPSEI---VELKHEIHVWRLTAQRISPA--- 565

Query: 254 SINSLEWNSRLELMNAQGSPCSNAHVENVETLRNSVSSKDNEIHRSHSAMTESARISDAS 313
                         + + +      +E V  L + ++ + +  HR         +IS   
Sbjct: 566 --------------SREETAVRGLLLEKVLALEHLLAQRLHTFHR---------QISQED 625

Query: 314 KEWVAIASPQKREEGLSSKSLNSMDKQKEAVSLQLQSSEGKEHWSTKWRKIAWKSCVYLV 373
           K W       +R+  +S +SL                                  C  L 
Sbjct: 626 KNWETNIQELQRKHRISDRSL-------------------------------LVKC--LT 685

Query: 374 TLGMLIALL--------MGLNMSWTAVTAALALVVL-DFKDAQPCLEKVSYSLLVFFCGM 433
            LG +I++         + L++ W A+  A+ L++L D  D +  L +V ++ L+FF  +
Sbjct: 686 VLGFVISMFFLNSFVPGIHLDLGWIAILGAIWLLILADIHDFEIILHRVEWATLLFFAAL 745

Query: 434 FMTVDGFNK------TGLPSAFWNFMEPHAQIDRVSGTVILALVILYL-SNLASNVP-TV 493
           F+ ++           G  +A    M P  Q  R +  ++L + +  L S+L  N+P T 
Sbjct: 746 FVLMEALTHLHLVEYVGEQTALLIKMVPEDQ--RFAAAIVLIVWVSALASSLIDNIPFTA 805

Query: 494 LLLGARVAASAAAISAAEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYN 548
            ++   +  S     +         LA  + + GN +L+G++ N++    A +     Y 
Sbjct: 806 TMIPVLLNLSQDPEISLPALPLMYALALGACLGGNGTLIGASTNVVCAGIAEKH---GYG 820

BLAST of Lag0020032 vs. ExPASy Swiss-Prot
Match: Q8MIQ9 (P protein OS=Sus scrofa OX=9823 GN=Oca2 PE=2 SV=3)

HSP 1 Score: 83.2 bits (204), Expect = 1.0e-14
Identity = 127/573 (22.16%), Postives = 233/573 (40.66%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLG-----AMLMVVFRVLTPD 60
           MA  +    I   +  A   L  V+  I F  + RT  ++LG     A L V+    T  
Sbjct: 325 MAHQYLRASIEAQVTIAAVILAGVYVLIIFEIVHRTLAAMLGSLAALAALAVIGDRPTLT 384

Query: 61  QAYAAIDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWRSRG-AKDLICRVCLISAISS 120
           Q    ID   L LLFG M++         F Y        SRG    +I  +CLI+A+ S
Sbjct: 385 QVVEWIDFETLALLFGMMILVAIFSETGFFDYCAVKAYQLSRGRVWAMIIMLCLIAAVLS 444

Query: 121 AFFTNDTSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSK 180
           AF  N T+ ++ T   +++    NL PR  L+A     NIG +AT IG+P N++I    +
Sbjct: 445 AFLDNVTTALLFTPVTIRLCEVLNLDPRQVLIAEVIFTNIGGAATAIGDPPNVIIVSNQE 504

Query: 181 -----IPFVQFVIGILPAMLVGIVVNALIILILYWKLLSVQKDEEDVSPEIIADEDVNSH 240
                + F  F   +   +   ++ +  ++ +LYW      K+  ++   +    +++  
Sbjct: 505 LRKMGLDFAGFTAHMFAGICFVLLFSFPLLRLLYWNRKLYNKEPSEI---VELKHEIHVW 564

Query: 241 RFSPARLSHSQIPSINSLEWNSRLELMNAQGSPCSNAHVENVETLRNSVSSKDNEIHRSH 300
           R +  R+S +                 + + +      +E V +L   ++ + +  HR  
Sbjct: 565 RLTAQRISPA-----------------SREETAVRGLLLEKVLSLERLLARRLHSFHR-- 624

Query: 301 SAMTESARISDASKEWVAIASPQKREEGLSSKSLNSMDKQKEAVSLQLQSSEGKEHWSTK 360
                  +IS   K W       +++  +S ++L +                        
Sbjct: 625 -------QISQEDKNWETNIQELQKKHRISDRTLLT------------------------ 684

Query: 361 WRKIAWKSCVYLVTLGMLIALL------MGLNMSWTAVTAALALVVL-DFKDAQPCLEKV 420
                   CV ++ L + +  L      + L++ W A+  A+ L++L D  D +  L +V
Sbjct: 685 -------KCVTVLGLVIFMFFLNSFVPGVHLDLGWIAILGAIWLLILADIHDFEIILHRV 744

Query: 421 SYSLLVFFCGMFMTVDG------FNKTGLPSAFWNFMEPHAQIDRVSGTVILAL-VILYL 480
            ++ L+FF  +F+ ++           G  +A    M P  Q  R++  +I+ + V    
Sbjct: 745 EWATLLFFAALFILMEALAHLHLIEYVGEQTALLIKMVPEDQ--RLAAAIIVVVWVSAIA 804

Query: 481 SNLASNVP-TVLLLGARVAASAAAISAAEEKRAWLLLAWISTVAGNLSLLGSAANLIVCE 540
           S+L  N+P T  ++   +  S     +         LA  + + GN +L+G++AN +VC 
Sbjct: 805 SSLIDNIPFTATMIPVLLNLSRDPEISLPAPPLMYALALGACLGGNGTLIGASAN-VVC- 832

Query: 541 QARRSPQLSYNLSFWNHLKFGLPSTLIVTAIGL 548
            A  + Q  Y  SF    + G P  ++   +G+
Sbjct: 865 -AGIAEQHGYGFSFMEFFRLGFPMMVVSCMVGM 832

BLAST of Lag0020032 vs. ExPASy TrEMBL
Match: A0A6J1CIT4 (silicon efflux transporter LSI2 OS=Momordica charantia OX=3673 GN=LOC111011742 PE=4 SV=1)

HSP 1 Score: 970.7 bits (2508), Expect = 2.6e-279
Identity = 506/551 (91.83%), Postives = 531/551 (96.37%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMV+FRVLTPDQAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVIFRVLTPDQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWRSRGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSW+SRGAKDLICRVC+ISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSRGAKDLICRVCVISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFVQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPF QF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180

Query: 181 VIGILPAMLVGIVVNALIILILYWKLLSVQKDEEDVSPEIIADEDVNSHRFSPARLSHSQ 240
           VIGILPAMLVGIVVNA+IIL+LYWKLLSVQKDEED   E++ADEDVNSHRFSPARLSH+ 
Sbjct: 181 VIGILPAMLVGIVVNAVIILVLYWKLLSVQKDEEDAPSEVVADEDVNSHRFSPARLSHTH 240

Query: 241 IPSINSLEWNSRLELMNAQGSPCSNAHVENVETLRNSVSSKDNEIHRSHSAMTESARISD 300
           IPSINS EWNSRL+ MNAQ SPC+NA++ENVET+RNS+SSKDNEIHRS SAMTESAR+SD
Sbjct: 241 IPSINSPEWNSRLDSMNAQSSPCANANIENVETVRNSISSKDNEIHRSLSAMTESARVSD 300

Query: 301 ASKEWVAIASPQKREEGLSSKSLNSMDKQKEAVSLQLQSSEGKEHWSTKWRKIAWKSCVY 360
           ASKEW+  +SPQKREE   SKS NSMDKQKE VS+ LQSSEGKEHWSTKWR+IAWK CVY
Sbjct: 301 ASKEWLPTSSPQKREESFPSKSFNSMDKQKELVSVSLQSSEGKEHWSTKWRRIAWKCCVY 360

Query: 361 LVTLGMLIALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF 420
           LVT+GML+ALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF
Sbjct: 361 LVTIGMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF 420

Query: 421 NKTGLPSAFWNFMEPHAQIDRVSGTVILALVILYLSNLASNVPTVLLLGARVAASAAAIS 480
           NKTGLPSAFWNFMEPHAQIDRVSGTV+LALVILYLSNLASNVPTVLLLGARVAASAAAIS
Sbjct: 421 NKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAIS 480

Query: 481 AAEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFGLPSTL 540
             +EKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFG+PSTL
Sbjct: 481 PDQEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFGVPSTL 540

Query: 541 IVTAIGLVLIR 552
           +VTAIGLVLIR
Sbjct: 541 LVTAIGLVLIR 551

BLAST of Lag0020032 vs. ExPASy TrEMBL
Match: A0A1S4DT02 (putative transporter arsB OS=Cucumis melo OX=3656 GN=LOC103484097 PE=4 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.4e-277
Identity = 511/551 (92.74%), Postives = 531/551 (96.37%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAMDHTVKVILGSIAFA+FWLLAVFPAIPFLPIGRTAGS+LGAMLMV+FRVLTP+QAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVIFRVLTPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWRSRGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSW+S+GAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFVQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPF QF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180

Query: 181 VIGILPAMLVGIVVNALIILILYWKLLSVQKDEEDVSPEIIADEDVNSHRFSPARLSHSQ 240
           VIGILPAMLVG+VVNALIILILYWKLLSVQKDEED SPE+IADEDV SHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240

Query: 241 IPSINSLEWNSRLELMNAQGSPCSNAHVENVETLRNSVSSKDNEIHRSHSAMTESARISD 300
           IPS+NS EW+SRL+ MNAQ  PCSNA   NVET+RNSVSSKDNEIHRSHS +TE ARISD
Sbjct: 241 IPSLNSAEWDSRLDSMNAQSPPCSNA---NVETIRNSVSSKDNEIHRSHSTLTEPARISD 300

Query: 301 ASKEWVAIASPQKREEGLSSKSLNSMDKQKEAVSLQLQSSEGKEHWSTKWRKIAWKSCVY 360
           ASKEW+  ASPQKREEG SSKSLNSMDKQKE V   LQSSEGKEHWSTKWR+IAWKSCVY
Sbjct: 301 ASKEWLPNASPQKREEGFSSKSLNSMDKQKEPVI--LQSSEGKEHWSTKWRRIAWKSCVY 360

Query: 361 LVTLGMLIALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF 420
           LVT+GMLIALL+GL+MSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF
Sbjct: 361 LVTVGMLIALLLGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF 420

Query: 421 NKTGLPSAFWNFMEPHAQIDRVSGTVILALVILYLSNLASNVPTVLLLGARVAASAAAIS 480
           NKTGLPSAFWNFMEPHAQIDRVSGTV+LALVILYLSNLASNVPTVLLLGARVAASAAAIS
Sbjct: 421 NKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAIS 480

Query: 481 AAEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFGLPSTL 540
            AEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARR+PQLSYNLSFWNHLKFGLPSTL
Sbjct: 481 PAEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTL 540

Query: 541 IVTAIGLVLIR 552
           IVTAIGLVLIR
Sbjct: 541 IVTAIGLVLIR 546

BLAST of Lag0020032 vs. ExPASy TrEMBL
Match: A0A5D3DH83 (Putative transporter arsB OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G00790 PE=4 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.4e-277
Identity = 511/551 (92.74%), Postives = 531/551 (96.37%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAMDHTVKVILGSIAFA+FWLLAVFPAIPFLPIGRTAGS+LGAMLMV+FRVLTP+QAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVIFRVLTPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWRSRGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSW+S+GAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFVQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPF QF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180

Query: 181 VIGILPAMLVGIVVNALIILILYWKLLSVQKDEEDVSPEIIADEDVNSHRFSPARLSHSQ 240
           VIGILPAMLVG+VVNALIILILYWKLLSVQKDEED SPE+IADEDV SHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240

Query: 241 IPSINSLEWNSRLELMNAQGSPCSNAHVENVETLRNSVSSKDNEIHRSHSAMTESARISD 300
           IPS+NS EW+SRL+ MNAQ  PCSNA   NVET+RNSVSSKDNEIHRSHS +TE ARISD
Sbjct: 241 IPSLNSAEWDSRLDSMNAQSPPCSNA---NVETIRNSVSSKDNEIHRSHSTLTEPARISD 300

Query: 301 ASKEWVAIASPQKREEGLSSKSLNSMDKQKEAVSLQLQSSEGKEHWSTKWRKIAWKSCVY 360
           ASKEW+  ASPQKREEG SSKSLNSMDKQKE V   LQSSEGKEHWSTKWR+IAWKSCVY
Sbjct: 301 ASKEWLPNASPQKREEGFSSKSLNSMDKQKEPVI--LQSSEGKEHWSTKWRRIAWKSCVY 360

Query: 361 LVTLGMLIALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF 420
           LVT+GMLIALL+GL+MSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF
Sbjct: 361 LVTVGMLIALLLGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF 420

Query: 421 NKTGLPSAFWNFMEPHAQIDRVSGTVILALVILYLSNLASNVPTVLLLGARVAASAAAIS 480
           NKTGLPSAFWNFMEPHAQIDRVSGTV+LALVILYLSNLASNVPTVLLLGARVAASAAAIS
Sbjct: 421 NKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAIS 480

Query: 481 AAEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFGLPSTL 540
            AEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARR+PQLSYNLSFWNHLKFGLPSTL
Sbjct: 481 PAEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTL 540

Query: 541 IVTAIGLVLIR 552
           IVTAIGLVLIR
Sbjct: 541 IVTAIGLVLIR 546

BLAST of Lag0020032 vs. ExPASy TrEMBL
Match: A0A1L7B7J1 (LSi2 OS=Cucumis sativus var. sativus OX=869827 GN=LSi2 PE=2 SV=1)

HSP 1 Score: 945.3 bits (2442), Expect = 1.2e-271
Identity = 505/552 (91.49%), Postives = 526/552 (95.29%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAMDHTVKVILGSIAFA+FWLLAVFPAIPFLPIGRTAGS+LGAMLMVVFRVLTP+QAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWRSRGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSW+S+GAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFVQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKI F QF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIHFGQF 180

Query: 181 VIGILPAMLVGIVVNALIILILYWKLLSVQKDEEDVSPEIIADEDVNSHRFSPARLSHSQ 240
           VIGILPAMLVG+VVNALIILI+YWKLLSVQKDEED SPE+IADEDV SHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240

Query: 241 IPSINSLEWNSRLELMNAQGSPCSNAHVENVETLRNSVSSKDN-EIHRSHSAMTESARIS 300
           IPS+NS EW+SRL+LMNAQ  PCSN    NVET+RNSVSSKDN EI RSHS MTE ARIS
Sbjct: 241 IPSLNSAEWDSRLDLMNAQSPPCSNT---NVETIRNSVSSKDNEEIRRSHSTMTEPARIS 300

Query: 301 DASKEWVAIASPQKREEGLSSKSLNSMDKQKEAVSLQLQSSEGKEHWSTKWRKIAWKSCV 360
           DASKEW+  AS QKREE  SSKSLNSM+KQKE V   LQSSEGKEHWSTKWR+IAWKSCV
Sbjct: 301 DASKEWLPNASTQKREEDFSSKSLNSMEKQKEPVI--LQSSEGKEHWSTKWRRIAWKSCV 360

Query: 361 YLVTLGMLIALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDG 420
           YLVT+GML+ALLMGL+MSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDG
Sbjct: 361 YLVTVGMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDG 420

Query: 421 FNKTGLPSAFWNFMEPHAQIDRVSGTVILALVILYLSNLASNVPTVLLLGARVAASAAAI 480
           FNKTGLPSAFWNFMEPHAQIDRVSGTV+LALVILYLSNLASNVPTVLLLGARVAASAAAI
Sbjct: 421 FNKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAI 480

Query: 481 SAAEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFGLPST 540
           S  EEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARR+PQLSYNLSFWNHLKFGLPST
Sbjct: 481 SPTEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPST 540

Query: 541 LIVTAIGLVLIR 552
           LIVTAIGLVLI+
Sbjct: 541 LIVTAIGLVLIK 547

BLAST of Lag0020032 vs. ExPASy TrEMBL
Match: A0A0A0L6L7 (CitMHS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G182780 PE=4 SV=1)

HSP 1 Score: 945.3 bits (2442), Expect = 1.2e-271
Identity = 505/552 (91.49%), Postives = 526/552 (95.29%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAMDHTVKVILGSIAFA+FWLLAVFPAIPFLPIGRTAGS+LGAMLMVVFRVLTP+QAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWRSRGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSW+S+GAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFVQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKI F QF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIHFGQF 180

Query: 181 VIGILPAMLVGIVVNALIILILYWKLLSVQKDEEDVSPEIIADEDVNSHRFSPARLSHSQ 240
           VIGILPAMLVG+VVNALIILI+YWKLLSVQKDEED SPE+IADEDV SHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240

Query: 241 IPSINSLEWNSRLELMNAQGSPCSNAHVENVETLRNSVSSKDN-EIHRSHSAMTESARIS 300
           IPS+NS EW+SRL+LMNAQ  PCSN    NVET+RNSVSSKDN EI RSHS MTE ARIS
Sbjct: 241 IPSLNSAEWDSRLDLMNAQSPPCSNT---NVETIRNSVSSKDNEEIRRSHSTMTEPARIS 300

Query: 301 DASKEWVAIASPQKREEGLSSKSLNSMDKQKEAVSLQLQSSEGKEHWSTKWRKIAWKSCV 360
           DASKEW+  AS QKREE  SSKSLNSM+KQKE V   LQSSEGKEHWSTKWR+IAWKSCV
Sbjct: 301 DASKEWLPNASTQKREEDFSSKSLNSMEKQKEPVI--LQSSEGKEHWSTKWRRIAWKSCV 360

Query: 361 YLVTLGMLIALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDG 420
           YLVT+GML+ALLMGL+MSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDG
Sbjct: 361 YLVTVGMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDG 420

Query: 421 FNKTGLPSAFWNFMEPHAQIDRVSGTVILALVILYLSNLASNVPTVLLLGARVAASAAAI 480
           FNKTGLPSAFWNFMEPHAQIDRVSGTV+LALVILYLSNLASNVPTVLLLGARVAASAAAI
Sbjct: 421 FNKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAI 480

Query: 481 SAAEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFGLPST 540
           S  EEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARR+PQLSYNLSFWNHLKFGLPST
Sbjct: 481 SPTEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPST 540

Query: 541 LIVTAIGLVLIR 552
           LIVTAIGLVLI+
Sbjct: 541 LIVTAIGLVLIK 547

BLAST of Lag0020032 vs. TAIR 10
Match: AT1G02260.1 (Divalent ion symporter )

HSP 1 Score: 640.6 bits (1651), Expect = 1.2e-183
Identity = 353/556 (63.49%), Postives = 418/556 (75.18%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAM   +K++LGS+AFAIFW+LAVFP++PFLPIGRTAGSL GAMLMV+F+V+TP+QAYAA
Sbjct: 1   MAMAPVIKLVLGSVAFAIFWILAVFPSVPFLPIGRTAGSLFGAMLMVIFQVITPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWRSRGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVS+YLERADMFKYLG +LSW+SRG KDL+CRVCL+SA+SSA FTND
Sbjct: 61  IDLPILGLLFGTMVVSIYLERADMFKYLGTLLSWKSRGPKDLLCRVCLVSAVSSALFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFVQF 180
           TSCVVLTEFVLKIARQ NLPP PFLLALA+SANIGSSATPIGNPQNLVIAVQSKIPF +F
Sbjct: 121 TSCVVLTEFVLKIARQKNLPPHPFLLALATSANIGSSATPIGNPQNLVIAVQSKIPFWEF 180

Query: 181 VIGILPAMLVGIVVNALIILILYWKLLSVQKDEED----VSPEIIADEDVNSHRFSPARL 240
           ++G+ PAM+VGI VNA+++L +YW+LLS  K++E+       E++A EDV SHRFSPA  
Sbjct: 181 LLGVFPAMIVGITVNAMLLLGMYWRLLSDHKEDEEEVQNADSEVVAQEDVQSHRFSPATF 240

Query: 241 SHSQIPSINSLEWNSRLELMNAQGSPCSNAHVENVETLRNSVSSKDNEIHRSHSAMTESA 300
           S                       S  SN  ++  ETLRN   S                
Sbjct: 241 S--------------------PVSSEDSNLRMDAAETLRNRAGSAG-------------- 300

Query: 301 RISDASKEWVAIASPQKREEGLSSKSLNSMDKQKEAVSLQLQSSEGKEHWSTK-WRKIAW 360
                             E  L S + N+  +Q      Q +S+     + TK WR++ W
Sbjct: 301 ------------------ESELISCNSNASREQHNDAESQGESNNTNNMFQTKRWRRVLW 360

Query: 361 KSCVYLVTLGMLIALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFM 420
           KS VY +TLGMLI+LLMGLNMSWTA+TAALALVVLDFKDA+P LEKVSYSLL+FFCGMF+
Sbjct: 361 KSSVYFITLGMLISLLMGLNMSWTAITAALALVVLDFKDARPSLEKVSYSLLIFFCGMFI 420

Query: 421 TVDGFNKTGLPSAFWNFMEPHAQIDRVSGTVILALVILYLSNLASNVPTVLLLGARVAAS 480
           TVDGFNKTG+P+A W+ MEP+A+ID+  G  +LA+VIL LSN+ASNVPTVLLLGARVAAS
Sbjct: 421 TVDGFNKTGIPTALWDLMEPYAKIDQAKGIAVLAVVILVLSNVASNVPTVLLLGARVAAS 480

Query: 481 AAAISAAEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFG 540
             A+   EEK+AWLLLAW+STVAGNL+LLGSAANLIVCEQARR+    Y L+F  H KFG
Sbjct: 481 --AMGREEEKKAWLLLAWVSTVAGNLTLLGSAANLIVCEQARRAVSHGYTLTFTKHFKFG 502

Query: 541 LPSTLIVTAIGLVLIR 552
           LPSTLIVTAIGL LI+
Sbjct: 541 LPSTLIVTAIGLFLIK 502

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038903236.12.2e-28094.01silicon efflux transporter LSI2 [Benincasa hispida] >XP_038903243.1 silicon effl... [more]
XP_022141316.15.3e-27991.83silicon efflux transporter LSI2 [Momordica charantia] >XP_022141318.1 silicon ef... [more]
XP_016899111.15.0e-27792.74PREDICTED: putative transporter arsB [Cucumis melo] >XP_016899112.1 PREDICTED: p... [more]
XP_004140769.12.4e-27191.49silicon efflux transporter LSI3 [Cucumis sativus] >XP_011651172.1 silicon efflux... [more]
KAG7013248.12.2e-26489.71putative transporter arsB, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q9AV234.5e-14853.79Silicon efflux transporter LSI3 OS=Oryza sativa subsp. japonica OX=39947 GN=LSI3... [more]
Q10SY91.5e-14352.85Silicon efflux transporter LSI2 OS=Oryza sativa subsp. japonica OX=39947 GN=LSI2... [more]
Q54GU06.3e-5731.27Putative transporter arsB OS=Dictyostelium discoideum OX=44689 GN=arsB PE=2 SV=1[more]
Q620524.6e-1522.06P protein OS=Mus musculus OX=10090 GN=Oca2 PE=1 SV=1[more]
Q8MIQ91.0e-1422.16P protein OS=Sus scrofa OX=9823 GN=Oca2 PE=2 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1CIT42.6e-27991.83silicon efflux transporter LSI2 OS=Momordica charantia OX=3673 GN=LOC111011742 P... [more]
A0A1S4DT022.4e-27792.74putative transporter arsB OS=Cucumis melo OX=3656 GN=LOC103484097 PE=4 SV=1[more]
A0A5D3DH832.4e-27792.74Putative transporter arsB OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1L7B7J11.2e-27191.49LSi2 OS=Cucumis sativus var. sativus OX=869827 GN=LSi2 PE=2 SV=1[more]
A0A0A0L6L71.2e-27191.49CitMHS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G182780 PE=4... [more]
Match NameE-valueIdentityDescription
AT1G02260.11.2e-18363.49Divalent ion symporter [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 550..551
NoneNo IPR availablePANTHERPTHR43302TRANSPORTER ARSB-RELATEDcoord: 1..551
NoneNo IPR availablePANTHERPTHR43302:SF12TRANSPORTER ARSB-RELATEDcoord: 1..551
NoneNo IPR availableCDDcd01117YbiR_permeasecoord: 28..550
e-value: 7.513E-111
score: 333.861
IPR004680Citrate transporter-like domainPFAMPF03600CitMHScoord: 30..498
e-value: 2.7E-54
score: 184.4

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0020032.1Lag0020032.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane