Lag0019984 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0019984
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionProtein DETOXIFICATION
Locationchr5: 47170714 .. 47171643 (+)
RNA-Seq ExpressionLag0019984
SyntenyLag0019984
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTCGTATAAACTGTTCCAACGCCGACGACTCTGAAATTCCTTACCTTAACTCAATTAATGAGTCTCTCAATCTCAATCTCAATCTCACGAACCCATTGATCCCTCAGCCTCCGACATCCCAATTACTAACCCACAAACCAACAAAACAAACTAATACTAATACCCATTTCTCCTTAGCCCTTCACGAGGCCTTCTCCATTTCCCGAATCGCCTTCCCCATGGTCCTCACCAGCCTCCTCCTCTACTCCCGCTCCCTCATCTCCATGCTCTTCCTCGGCCGTCTCGGCGACCTCCCCCTCGCCGGCGGCTCCCTCGCCATCGCCTTCGCCAACATCACCGGCTACTCCATTCTCTCCGGCCTCGCCATCGGCATGGAGCCCATCTGCGCCCAGGCCTTCGGCGCCAAGAAATTCTCCCTCCTCGGCCTCGCCCTTCAGAGGACCATTCTTCTCCTCTCCTTGGTCTCAATCCCCATTTCTATTCTCTGGCTCAACGTCGAGCCCATCCTTGTGCTCTGCAAACAGGACCGATCCATTGCCTCCCAAGCCCAACTCTTCCTTCTCTACTCTGTTCCTGACCTCCTCGCGCAGTCCCTCATCCACCCGCTTCGGATTTACCTTCGAAGCCAATCCATAACGCTTCCTCTCACTTTCTGCGCCACTTTTTCGATACTTCTCCACATACCCATCAATTATTTCCTCGTCTCCTACCTCGATTTCGGCATTCGAGGAGTTGCCATGGCAGGGGTCTGGACCAATTTCAACTTGGTGGCTTCTTTGATTCTCTACATAGTGATATTCCGCGTCCACCAGGAAACCTGGGGAGGGTTCTCTCTCGAATGCTTTAAAGAATGGGGAAGCTTCTCAATTTGGCTATCCCGAGCTGCGTGTCGGTCTGCCTCGAGTGGTGGTGGTACGAAATCATGA

mRNA sequence

ATGTGTCGTATAAACTGTTCCAACGCCGACGACTCTGAAATTCCTTACCTTAACTCAATTAATGAGTCTCTCAATCTCAATCTCAATCTCACGAACCCATTGATCCCTCAGCCTCCGACATCCCAATTACTAACCCACAAACCAACAAAACAAACTAATACTAATACCCATTTCTCCTTAGCCCTTCACGAGGCCTTCTCCATTTCCCGAATCGCCTTCCCCATGGTCCTCACCAGCCTCCTCCTCTACTCCCGCTCCCTCATCTCCATGCTCTTCCTCGGCCGTCTCGGCGACCTCCCCCTCGCCGGCGGCTCCCTCGCCATCGCCTTCGCCAACATCACCGGCTACTCCATTCTCTCCGGCCTCGCCATCGGCATGGAGCCCATCTGCGCCCAGGCCTTCGGCGCCAAGAAATTCTCCCTCCTCGGCCTCGCCCTTCAGAGGACCATTCTTCTCCTCTCCTTGGTCTCAATCCCCATTTCTATTCTCTGGCTCAACGTCGAGCCCATCCTTGTGCTCTGCAAACAGGACCGATCCATTGCCTCCCAAGCCCAACTCTTCCTTCTCTACTCTGTTCCTGACCTCCTCGCGCAGTCCCTCATCCACCCGCTTCGGATTTACCTTCGAAGCCAATCCATAACGCTTCCTCTCACTTTCTGCGCCACTTTTTCGATACTTCTCCACATACCCATCAATTATTTCCTCGTCTCCTACCTCGATTTCGGCATTCGAGGAGTTGCCATGGCAGGGGTCTGGACCAATTTCAACTTGGTGGCTTCTTTGATTCTCTACATAGTGATATTCCGCGTCCACCAGGAAACCTGGGGAGGGTTCTCTCTCGAATGCTTTAAAGAATGGGGAAGCTTCTCAATTTGGCTATCCCGAGCTGCGTGTCGGTCTGCCTCGAGTGGTGGTGGTACGAAATCATGA

Coding sequence (CDS)

ATGTGTCGTATAAACTGTTCCAACGCCGACGACTCTGAAATTCCTTACCTTAACTCAATTAATGAGTCTCTCAATCTCAATCTCAATCTCACGAACCCATTGATCCCTCAGCCTCCGACATCCCAATTACTAACCCACAAACCAACAAAACAAACTAATACTAATACCCATTTCTCCTTAGCCCTTCACGAGGCCTTCTCCATTTCCCGAATCGCCTTCCCCATGGTCCTCACCAGCCTCCTCCTCTACTCCCGCTCCCTCATCTCCATGCTCTTCCTCGGCCGTCTCGGCGACCTCCCCCTCGCCGGCGGCTCCCTCGCCATCGCCTTCGCCAACATCACCGGCTACTCCATTCTCTCCGGCCTCGCCATCGGCATGGAGCCCATCTGCGCCCAGGCCTTCGGCGCCAAGAAATTCTCCCTCCTCGGCCTCGCCCTTCAGAGGACCATTCTTCTCCTCTCCTTGGTCTCAATCCCCATTTCTATTCTCTGGCTCAACGTCGAGCCCATCCTTGTGCTCTGCAAACAGGACCGATCCATTGCCTCCCAAGCCCAACTCTTCCTTCTCTACTCTGTTCCTGACCTCCTCGCGCAGTCCCTCATCCACCCGCTTCGGATTTACCTTCGAAGCCAATCCATAACGCTTCCTCTCACTTTCTGCGCCACTTTTTCGATACTTCTCCACATACCCATCAATTATTTCCTCGTCTCCTACCTCGATTTCGGCATTCGAGGAGTTGCCATGGCAGGGGTCTGGACCAATTTCAACTTGGTGGCTTCTTTGATTCTCTACATAGTGATATTCCGCGTCCACCAGGAAACCTGGGGAGGGTTCTCTCTCGAATGCTTTAAAGAATGGGGAAGCTTCTCAATTTGGCTATCCCGAGCTGCGTGTCGGTCTGCCTCGAGTGGTGGTGGTACGAAATCATGA

Protein sequence

MCRINCSNADDSEIPYLNSINESLNLNLNLTNPLIPQPPTSQLLTHKPTKQTNTNTHFSLALHEAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDLPLAGGSLAIAFANITGYSILSGLAIGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISILWLNVEPILVLCKQDRSIASQAQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATFSILLHIPINYFLVSYLDFGIRGVAMAGVWTNFNLVASLILYIVIFRVHQETWGGFSLECFKEWGSFSIWLSRAACRSASSGGGTKS
Homology
BLAST of Lag0019984 vs. NCBI nr
Match: KAG6574153.1 (Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013207.1 Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 456.8 bits (1174), Expect = 1.4e-124
Identity = 244/293 (83.28%), Postives = 264/293 (90.10%), Query Frame = 0

Query: 1   MCRINCSN-----ADDSEIPYLNSINESLN-LNLNLTNPLIPQPPTSQLLTHKPTKQTNT 60
           MCR++CSN      DD E+ YLNSIN SL   N++ TNPLIPQPPTSQ   HKPTKQ   
Sbjct: 1   MCRVSCSNPSRLCGDDDEVAYLNSINHSLKPSNMHFTNPLIPQPPTSQQTHHKPTKQ--I 60

Query: 61  NTHFSLALHEAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDLPLAGGSLAIAFANIT 120
           +THFSLAL EAFSI++IAFPM+LTSLLLYSRSLISMLFLGRLG+L LAGGSLAI FANIT
Sbjct: 61  DTHFSLALGEAFSIAQIAFPMILTSLLLYSRSLISMLFLGRLGELSLAGGSLAIGFANIT 120

Query: 121 GYSILSGLAIGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISILWLNVEPILVLC 180
           GYSILSGLAIGMEPIC QAFGAKKF+LLGLALQRTILLLSL+SIPISILWLNV+PIL++C
Sbjct: 121 GYSILSGLAIGMEPICGQAFGAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLIC 180

Query: 181 KQDRSIASQAQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATFSILLHIPINYF 240
           KQD SIA  AQLFLLYSVPDL AQSLIHPLRIYLR+QSITLPLTFCATFSI LHIPINYF
Sbjct: 181 KQDESIAHHAQLFLLYSVPDLFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYF 240

Query: 241 LVSYLDFGIRGVAMAGVWTNFNLVASLILYIVIFRVHQETWGGFSLECFKEWG 288
           LVSYL+ GIRGVA+AGVWTNFNLVASLILYIVIF+VH+ETWGGFSLECFKEWG
Sbjct: 241 LVSYLNLGIRGVAIAGVWTNFNLVASLILYIVIFQVHKETWGGFSLECFKEWG 291

BLAST of Lag0019984 vs. NCBI nr
Match: XP_023542378.1 (protein DETOXIFICATION 49-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 456.1 bits (1172), Expect = 2.5e-124
Identity = 243/293 (82.94%), Postives = 264/293 (90.10%), Query Frame = 0

Query: 1   MCRINCSN-----ADDSEIPYLNSINESLN-LNLNLTNPLIPQPPTSQLLTHKPTKQTNT 60
           MCR++CSN      DD E+ YLNSIN SL   N++ TNPLIPQPPTSQ   HKPTKQ   
Sbjct: 1   MCRVSCSNPSRLCGDDDEVAYLNSINHSLKPSNMHFTNPLIPQPPTSQQTHHKPTKQ--I 60

Query: 61  NTHFSLALHEAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDLPLAGGSLAIAFANIT 120
           +THFSLAL EAFSI++IAFPM+LTSLLLYSRSLISMLFLGRLG+L LAGGSLA+ FANIT
Sbjct: 61  DTHFSLALGEAFSIAQIAFPMILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANIT 120

Query: 121 GYSILSGLAIGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISILWLNVEPILVLC 180
           GYSILSGLAIGMEPIC QAFGAKKF+LLGLALQRTILLLSL+SIPISILWLNV+PIL++C
Sbjct: 121 GYSILSGLAIGMEPICGQAFGAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLIC 180

Query: 181 KQDRSIASQAQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATFSILLHIPINYF 240
           KQD SIA  AQLFLLYSVPDL AQSLIHPLRIYLR+QSITLPLTFCATFSI LHIPINYF
Sbjct: 181 KQDESIAHHAQLFLLYSVPDLFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYF 240

Query: 241 LVSYLDFGIRGVAMAGVWTNFNLVASLILYIVIFRVHQETWGGFSLECFKEWG 288
           LVSYL+ GIRGVA+AGVWTNFNLVASLILYIVIF+VH+ETWGGFSLECFKEWG
Sbjct: 241 LVSYLNLGIRGVAIAGVWTNFNLVASLILYIVIFQVHKETWGGFSLECFKEWG 291

BLAST of Lag0019984 vs. NCBI nr
Match: XP_022944944.1 (protein DETOXIFICATION 49-like [Cucurbita moschata])

HSP 1 Score: 455.7 bits (1171), Expect = 3.2e-124
Identity = 243/293 (82.94%), Postives = 264/293 (90.10%), Query Frame = 0

Query: 1   MCRINCSN-----ADDSEIPYLNSINESLN-LNLNLTNPLIPQPPTSQLLTHKPTKQTNT 60
           MCR++CSN      DD E+ YLNSIN SL   N++ TNPLIPQPPTSQ   HKPTKQ   
Sbjct: 1   MCRVSCSNPSRLCGDDDEVAYLNSINHSLKPSNMHFTNPLIPQPPTSQQSHHKPTKQ--I 60

Query: 61  NTHFSLALHEAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDLPLAGGSLAIAFANIT 120
           +THFSLAL EAFSI++IAFPM+LTSLLLYSRSLISMLFLGRLG+L LAGGSLA+ FANIT
Sbjct: 61  DTHFSLALGEAFSIAQIAFPMILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANIT 120

Query: 121 GYSILSGLAIGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISILWLNVEPILVLC 180
           GYSILSGLAIGMEPIC QAFGAKKF+LLGLALQRTILLLSL+SIPISILWLNV+PIL++C
Sbjct: 121 GYSILSGLAIGMEPICGQAFGAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLIC 180

Query: 181 KQDRSIASQAQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATFSILLHIPINYF 240
           KQD SIA  AQLFLLYSVPDL AQSLIHPLRIYLR+QSITLPLTFCATFSI LHIPINYF
Sbjct: 181 KQDESIAHHAQLFLLYSVPDLFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYF 240

Query: 241 LVSYLDFGIRGVAMAGVWTNFNLVASLILYIVIFRVHQETWGGFSLECFKEWG 288
           LVSYL+ GIRGVA+AGVWTNFNLVASLILYIVIF+VH+ETWGGFSLECFKEWG
Sbjct: 241 LVSYLNLGIRGVAIAGVWTNFNLVASLILYIVIFQVHKETWGGFSLECFKEWG 291

BLAST of Lag0019984 vs. NCBI nr
Match: XP_022968021.1 (protein DETOXIFICATION 49-like [Cucurbita maxima])

HSP 1 Score: 452.2 bits (1162), Expect = 3.6e-123
Identity = 242/293 (82.59%), Postives = 263/293 (89.76%), Query Frame = 0

Query: 1   MCRINCSN-----ADDSEIPYLNSINESLN-LNLNLTNPLIPQPPTSQLLTHKPTKQTNT 60
           MCR++CSN      DD E+ YL SIN SL   N++ TNPLIPQPPTSQ   HKPTKQ   
Sbjct: 1   MCRVSCSNPSRLCGDDDEVAYLTSINHSLKPSNMHFTNPLIPQPPTSQQSHHKPTKQ--I 60

Query: 61  NTHFSLALHEAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDLPLAGGSLAIAFANIT 120
           +THFSLAL EAFSI++IAFPM+LTSLLLYSRSLISMLFLGRLG+L LAGGSLAI FANIT
Sbjct: 61  DTHFSLALGEAFSIAQIAFPMILTSLLLYSRSLISMLFLGRLGELSLAGGSLAIGFANIT 120

Query: 121 GYSILSGLAIGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISILWLNVEPILVLC 180
           GYSILSGLAIGMEPIC QAFGAKKF+LLGLALQRTILLLSL+SIPISILWLNV+PIL++C
Sbjct: 121 GYSILSGLAIGMEPICGQAFGAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLIC 180

Query: 181 KQDRSIASQAQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATFSILLHIPINYF 240
           KQD SIA  AQLFLLYSVPDL AQSLIHPLRIYLR+QSITLPLTFCATFSI LHIPINYF
Sbjct: 181 KQDESIAHHAQLFLLYSVPDLFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYF 240

Query: 241 LVSYLDFGIRGVAMAGVWTNFNLVASLILYIVIFRVHQETWGGFSLECFKEWG 288
           LVS+L+ GIRGVA+AGVWTNFNLVASLILYIVIF+VH+ETWGGFSLECFKEWG
Sbjct: 241 LVSHLNLGIRGVAIAGVWTNFNLVASLILYIVIFQVHKETWGGFSLECFKEWG 291

BLAST of Lag0019984 vs. NCBI nr
Match: XP_038880058.1 (protein DETOXIFICATION 49 [Benincasa hispida])

HSP 1 Score: 450.7 bits (1158), Expect = 1.0e-122
Identity = 245/299 (81.94%), Postives = 265/299 (88.63%), Query Frame = 0

Query: 1   MCRINCSNAD---DSEIPYLNSINESLN----LNLNLTNPLIPQPPTSQLLTHKPTKQ-- 60
           MCR+ CSN     + E+ YLNSIN+S N    +  +LTNPLIPQ PTSQ   HKPTKQ  
Sbjct: 1   MCRVRCSNCSKLCNCEVSYLNSINDSENPTNSIMHHLTNPLIPQAPTSQQF-HKPTKQTA 60

Query: 61  ---TNTNTHFSLALHEAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDLPLAGGSLAI 120
              TN+N+HFSLA+ EAFSIS+IAFPMVLTSLLLYSRSLISMLFLGRLG+LPLAGGSLAI
Sbjct: 61  ITNTNSNSHFSLAIQEAFSISQIAFPMVLTSLLLYSRSLISMLFLGRLGELPLAGGSLAI 120

Query: 121 AFANITGYSILSGLAIGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISILWLNVE 180
            FANITGYSILSGLAIGMEPIC QAFGAKKF+LLGLALQRTILLLSL SIPIS+LW NV+
Sbjct: 121 GFANITGYSILSGLAIGMEPICGQAFGAKKFTLLGLALQRTILLLSLASIPISLLWFNVK 180

Query: 181 PILVLCKQDRSIASQAQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATFSILLH 240
            IL+LCKQD SIASQAQLFLLYSVPDL AQSLIHPLRIYLRSQSITLPLTFCA FSILLH
Sbjct: 181 NILLLCKQDHSIASQAQLFLLYSVPDLFAQSLIHPLRIYLRSQSITLPLTFCAAFSILLH 240

Query: 241 IPINYFLVSYLDFGIRGVAMAGVWTNFNLVASLILYIVIFRVHQETWGGFSLECFKEWG 288
           IPINY LVSYLDFGIRGVA+AGVWTNFNLVASLILYI+IFRVH++TWGGFSL+CFKEWG
Sbjct: 241 IPINYLLVSYLDFGIRGVAIAGVWTNFNLVASLILYILIFRVHKDTWGGFSLQCFKEWG 298

BLAST of Lag0019984 vs. ExPASy Swiss-Prot
Match: O82752 (Protein DETOXIFICATION 49 OS=Arabidopsis thaliana OX=3702 GN=DTX49 PE=2 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 3.5e-81
Identity = 155/234 (66.24%), Postives = 195/234 (83.33%), Query Frame = 0

Query: 56  THFSLALHEAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDL-PLAGGSLAIAFANIT 115
           TH S ++ EA SI++I+ P++LT LLLYSRS+ISMLFLGRL DL  L+GGSLA+ FANIT
Sbjct: 24  THLSSSIQEAKSIAKISLPLILTGLLLYSRSMISMLFLGRLNDLSALSGGSLALGFANIT 83

Query: 116 GYSILSGLAIGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISILWLNVEPILVLC 175
           GYS+LSGL+IGMEPIC QAFGAK+F LLGLALQRT LLL L S+PISILWLN++ IL+  
Sbjct: 84  GYSLLSGLSIGMEPICVQAFGAKRFKLLGLALQRTTLLLLLCSLPISILWLNIKKILLFF 143

Query: 176 KQDRSIASQAQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATFSILLHIPINYF 235
            QD  I++QA++F+L+S+PDL+ QS +HP+RIYLRSQSITLPLT+ A F++LLHIPINY 
Sbjct: 144 GQDEEISNQAEIFILFSLPDLILQSFLHPIRIYLRSQSITLPLTYSAFFAVLLHIPINYL 203

Query: 236 LVSYLDFGIRGVAMAGVWTNFNLVASLILYIVIFRVHQETWGGFSLECFKEWGS 289
           LVS L  G++GVA+  +WTN NL+  LI+YIV   V+Q+TWGGFS++CFK W S
Sbjct: 204 LVSSLGLGLKGVALGAIWTNVNLLGFLIIYIVFSGVYQKTWGGFSMDCFKGWRS 257

BLAST of Lag0019984 vs. ExPASy Swiss-Prot
Match: Q9SLV0 (Protein DETOXIFICATION 48 OS=Arabidopsis thaliana OX=3702 GN=DTX48 PE=2 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 8.7e-64
Identity = 128/231 (55.41%), Postives = 169/231 (73.16%), Query Frame = 0

Query: 58  FSLALHEAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDLPLAGGSLAIAFANITGYS 117
           F   L E  +I +I+ P  +T LL+YSR++ISMLFLG LG+L LAGGSL+I FANITGYS
Sbjct: 52  FLEGLEEVKAIGKISGPTAMTGLLMYSRAMISMLFLGYLGELELAGGSLSIGFANITGYS 111

Query: 118 ILSGLAIGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISILWLNVEPILVLCKQD 177
           ++SGL++GMEPIC QA+GAK+  LLGL LQRT+LLL   S+PIS  WLN+  IL+ C QD
Sbjct: 112 VISGLSMGMEPICGQAYGAKQMKLLGLTLQRTVLLLLSCSVPISFSWLNMRRILLWCGQD 171

Query: 178 RSIASQAQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATFSILLHIPINYFLVS 237
             I+S AQ FLL+++PDL   SL+HPLRIYLR+Q+ITLP+T+    S+LLH+P+NY LV 
Sbjct: 172 EEISSVAQQFLLFAIPDLFLLSLLHPLRIYLRTQNITLPVTYSTAVSVLLHVPLNYLLVV 231

Query: 238 YLDFGIRGVAMAGVWTNFNLVASLILYIVIFRVHQETWGGFSLECFKEWGS 289
            L+ G+ GVA+A V TN NLV  L  ++    VH +TW   +++  K W +
Sbjct: 232 KLEMGVAGVAIAMVLTNLNLVVLLSSFVYFTSVHSDTWVPITIDSLKGWSA 282

BLAST of Lag0019984 vs. ExPASy Swiss-Prot
Match: Q9FJ87 (Protein DETOXIFICATION 50 OS=Arabidopsis thaliana OX=3702 GN=DTX50 PE=2 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 4.3e-63
Identity = 125/225 (55.56%), Postives = 175/225 (77.78%), Query Frame = 0

Query: 44  LTHKPTKQTNTNTHFSLALHEAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDLPLAG 103
           L  K +   N ++  S+ L+EA SI +I++P+VLT L LY RS +S+ FLG LGD  LAG
Sbjct: 15  LLQKTSHLKNHSSVLSVFLNEAISICKISYPLVLTGLFLYVRSFVSLSFLGGLGDATLAG 74

Query: 104 GSLAIAFANITGYSILSGLAIGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISIL 163
           GSLA AFANITGYS+ SGL +G+E IC+QAFGA++++ +  +++R I+LL + S+P+++L
Sbjct: 75  GSLAAAFANITGYSLFSGLTMGVESICSQAFGARRYNYVCASVKRGIILLLVTSLPVTLL 134

Query: 164 WLNVEPILVLCKQDRSIASQAQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATF 223
           W+N+E IL++ KQD+ +AS+A +FLLYSVPDL+AQS +HPLR+YLR+QS TLPL+ C   
Sbjct: 135 WMNMEKILLILKQDKKLASEAHIFLLYSVPDLVAQSFLHPLRVYLRTQSKTLPLSICTVI 194

Query: 224 SILLHIPINYFLVSYLDFGIRGVAMAGVWTNFNLVASLILYIVIF 269
           +  LH+PI +FLVSYL  GI+G+A++GV +NFNLVA L LYI  F
Sbjct: 195 ASFLHLPITFFLVSYLGLGIKGIALSGVVSNFNLVAFLFLYICFF 239

BLAST of Lag0019984 vs. ExPASy Swiss-Prot
Match: Q4PSF4 (Protein DETOXIFICATION 52 OS=Arabidopsis thaliana OX=3702 GN=DTX52 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 6.9e-61
Identity = 125/224 (55.80%), Postives = 163/224 (72.77%), Query Frame = 0

Query: 64  EAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDLPLAGGSLAIAFANITGYSILSGLA 123
           EA S+  +AFP +L +L+LY+RS ISMLFLG +G+L LAGGSLAIAFANITGYS+L+GLA
Sbjct: 36  EARSLFSLAFPTILAALILYARSAISMLFLGHIGELELAGGSLAIAFANITGYSVLAGLA 95

Query: 124 IGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISILWLNVEPILVLCKQDRSIASQ 183
           +GM+P+C+QAFGA +  LL L LQRT+L L   S+ I  LWLN+  I++   QD SI+S 
Sbjct: 96  LGMDPLCSQAFGAGRPKLLSLTLQRTVLFLLTSSVVIVALWLNLGKIMIYLHQDPSISSL 155

Query: 184 AQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATFSILLHIPINYFLVSYLDFGI 243
           AQ ++L S+PDLL  S +HPLRIYLR+Q IT PLT       + HIP+N+FLVSYL +G 
Sbjct: 156 AQTYILCSIPDLLTNSFLHPLRIYLRAQGITSPLTLATLAGTIFHIPMNFFLVSYLGWGF 215

Query: 244 RGVAMAGVWTNFNLVASLILYIVIFRVHQETWGGFSLECFKEWG 288
            GV+MA   +N  +V  L+ ++ I  +HQ TW   S ECFK+WG
Sbjct: 216 MGVSMAAAASNLLVVIFLVAHVWIAGLHQPTWTRPSSECFKDWG 259

BLAST of Lag0019984 vs. ExPASy Swiss-Prot
Match: Q9SZE2 (Protein DETOXIFICATION 51 OS=Arabidopsis thaliana OX=3702 GN=DTX51 PE=2 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.9e-58
Identity = 120/226 (53.10%), Postives = 160/226 (70.80%), Query Frame = 0

Query: 61  ALHEAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDLPLAGGSLAIAFANITGYSILS 120
           A+ EA S+  +AFP+ +T+L+LY RS +SM FLG+LGDL LA GSLAIAFANITGYS+LS
Sbjct: 53  AVTEAKSLFTLAFPIAVTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAFANITGYSVLS 112

Query: 121 GLAIGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISILWLNVEPILVLCKQDRSI 180
           GLA+GMEP+C+QAFGA +F LL L L RT++ L +  +PIS+LW NV  I V   QD  I
Sbjct: 113 GLALGMEPLCSQAFGAHRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKISVYLHQDPDI 172

Query: 181 ASQAQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATFSILLHIPINYFLVSYLD 240
           A  AQ +L++S+PDLL  +L+HP+RIYLR+Q I  P+T  +    + H+P N FLVSYL 
Sbjct: 173 AKLAQTYLIFSLPDLLTNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLPANLFLVSYLR 232

Query: 241 FGIRGVAMAGVWTNFNLVASLILYIVIFRVHQETWGGFSLECFKEW 287
            G+ GVA+A   TN  +VA L+ Y+    +H  TW   + +CF+ W
Sbjct: 233 LGLTGVAVASSITNIFVVAFLVCYVWASGLHAPTWTDPTRDCFRGW 278

BLAST of Lag0019984 vs. ExPASy TrEMBL
Match: A0A6J1FZK1 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111449327 PE=3 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.6e-124
Identity = 243/293 (82.94%), Postives = 264/293 (90.10%), Query Frame = 0

Query: 1   MCRINCSN-----ADDSEIPYLNSINESLN-LNLNLTNPLIPQPPTSQLLTHKPTKQTNT 60
           MCR++CSN      DD E+ YLNSIN SL   N++ TNPLIPQPPTSQ   HKPTKQ   
Sbjct: 1   MCRVSCSNPSRLCGDDDEVAYLNSINHSLKPSNMHFTNPLIPQPPTSQQSHHKPTKQ--I 60

Query: 61  NTHFSLALHEAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDLPLAGGSLAIAFANIT 120
           +THFSLAL EAFSI++IAFPM+LTSLLLYSRSLISMLFLGRLG+L LAGGSLA+ FANIT
Sbjct: 61  DTHFSLALGEAFSIAQIAFPMILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANIT 120

Query: 121 GYSILSGLAIGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISILWLNVEPILVLC 180
           GYSILSGLAIGMEPIC QAFGAKKF+LLGLALQRTILLLSL+SIPISILWLNV+PIL++C
Sbjct: 121 GYSILSGLAIGMEPICGQAFGAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLIC 180

Query: 181 KQDRSIASQAQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATFSILLHIPINYF 240
           KQD SIA  AQLFLLYSVPDL AQSLIHPLRIYLR+QSITLPLTFCATFSI LHIPINYF
Sbjct: 181 KQDESIAHHAQLFLLYSVPDLFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYF 240

Query: 241 LVSYLDFGIRGVAMAGVWTNFNLVASLILYIVIFRVHQETWGGFSLECFKEWG 288
           LVSYL+ GIRGVA+AGVWTNFNLVASLILYIVIF+VH+ETWGGFSLECFKEWG
Sbjct: 241 LVSYLNLGIRGVAIAGVWTNFNLVASLILYIVIFQVHKETWGGFSLECFKEWG 291

BLAST of Lag0019984 vs. ExPASy TrEMBL
Match: A0A6J1HTP9 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111467391 PE=3 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.7e-123
Identity = 242/293 (82.59%), Postives = 263/293 (89.76%), Query Frame = 0

Query: 1   MCRINCSN-----ADDSEIPYLNSINESLN-LNLNLTNPLIPQPPTSQLLTHKPTKQTNT 60
           MCR++CSN      DD E+ YL SIN SL   N++ TNPLIPQPPTSQ   HKPTKQ   
Sbjct: 1   MCRVSCSNPSRLCGDDDEVAYLTSINHSLKPSNMHFTNPLIPQPPTSQQSHHKPTKQ--I 60

Query: 61  NTHFSLALHEAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDLPLAGGSLAIAFANIT 120
           +THFSLAL EAFSI++IAFPM+LTSLLLYSRSLISMLFLGRLG+L LAGGSLAI FANIT
Sbjct: 61  DTHFSLALGEAFSIAQIAFPMILTSLLLYSRSLISMLFLGRLGELSLAGGSLAIGFANIT 120

Query: 121 GYSILSGLAIGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISILWLNVEPILVLC 180
           GYSILSGLAIGMEPIC QAFGAKKF+LLGLALQRTILLLSL+SIPISILWLNV+PIL++C
Sbjct: 121 GYSILSGLAIGMEPICGQAFGAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLIC 180

Query: 181 KQDRSIASQAQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATFSILLHIPINYF 240
           KQD SIA  AQLFLLYSVPDL AQSLIHPLRIYLR+QSITLPLTFCATFSI LHIPINYF
Sbjct: 181 KQDESIAHHAQLFLLYSVPDLFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYF 240

Query: 241 LVSYLDFGIRGVAMAGVWTNFNLVASLILYIVIFRVHQETWGGFSLECFKEWG 288
           LVS+L+ GIRGVA+AGVWTNFNLVASLILYIVIF+VH+ETWGGFSLECFKEWG
Sbjct: 241 LVSHLNLGIRGVAIAGVWTNFNLVASLILYIVIFQVHKETWGGFSLECFKEWG 291

BLAST of Lag0019984 vs. ExPASy TrEMBL
Match: A0A6J1E4L7 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111430734 PE=3 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 1.0e-115
Identity = 232/287 (80.84%), Postives = 252/287 (87.80%), Query Frame = 0

Query: 12  SEIPYLNSINESL------NLNLN----LTNPLIPQPPTSQLLTHKPTKQTNTNTHFSLA 71
           SE+ YL+SIN+ L      N N N    LT+PLIPQ        HKPT  TNTNTHFSLA
Sbjct: 4   SEVAYLDSINDDLEKPTNTNNNTNSMHHLTDPLIPQQN-----RHKPTNNTNTNTHFSLA 63

Query: 72  LHEAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDLPLAGGSLAIAFANITGYSILSG 131
           + EAFSI +IAFPMVLTSLLLYSRSLISMLFLGRLG+LPLAGGSLAI FANITGYSILSG
Sbjct: 64  IKEAFSIFQIAFPMVLTSLLLYSRSLISMLFLGRLGELPLAGGSLAIGFANITGYSILSG 123

Query: 132 LAIGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISILWLNVEPILVLCKQDRSIA 191
           LA+GMEPIC QAFGAK+F+LLGLALQRTILLL+  SIPIS+LW NV+ IL+LCKQD SIA
Sbjct: 124 LAMGMEPICGQAFGAKRFNLLGLALQRTILLLTFASIPISLLWFNVKNILLLCKQDASIA 183

Query: 192 SQAQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATFSILLHIPINYFLVSYLDF 251
           SQAQLFLLYSVPDL AQSLIHPLRIYLR+QSITLPLTFCATFSILLHIPINYFLVSYL+F
Sbjct: 184 SQAQLFLLYSVPDLFAQSLIHPLRIYLRTQSITLPLTFCATFSILLHIPINYFLVSYLNF 243

Query: 252 GIRGVAMAGVWTNFNLVASLILYIVIFRVHQETWGGFSLECFKEWGS 289
           GIRGVA+AGVWTNFNLVASLILYI+IF VH+ETWGGFSLECFKEWG+
Sbjct: 244 GIRGVAIAGVWTNFNLVASLILYILIFNVHKETWGGFSLECFKEWGN 285

BLAST of Lag0019984 vs. ExPASy TrEMBL
Match: A0A0A0L6E5 (Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_3G180310 PE=3 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 5.6e-114
Identity = 228/281 (81.14%), Postives = 251/281 (89.32%), Query Frame = 0

Query: 7   SNADDSEIPYLNSINESLNLNLNLTNPLIPQPPTSQLLTHKPTKQTNTNTHFSLALHEAF 66
           +N + +  P  N  + S ++  +LTNPLIP  PTSQ     PTKQT TNTHFSLA+ EAF
Sbjct: 38  TNPNPNPNPIPNPNSNSNSIMHHLTNPLIPPLPTSQHF-QLPTKQT-TNTHFSLAIQEAF 97

Query: 67  SISRIAFPMVLTSLLLYSRSLISMLFLGRLGDLPLAGGSLAIAFANITGYSILSGLAIGM 126
           SIS+IAFPMVLTSLLLYSRSLISMLFLGRLG+LPLAGGSLAI FANITGYSILSGLA+GM
Sbjct: 98  SISQIAFPMVLTSLLLYSRSLISMLFLGRLGELPLAGGSLAIGFANITGYSILSGLAMGM 157

Query: 127 EPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISILWLNVEPILVLCKQDRSIASQAQL 186
           EPIC QAFGAKKF+LLGLALQRTILLLSL SIPI+ LW NV+ IL+LCKQD SIASQAQL
Sbjct: 158 EPICGQAFGAKKFTLLGLALQRTILLLSLTSIPIAFLWFNVKNILLLCKQDPSIASQAQL 217

Query: 187 FLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATFSILLHIPINYFLVSYLDFGIRGV 246
           FLLYS+PDL AQSLIHPLRIYLRSQSITLPLTFCA FSILLHIPINYFLVSYL+FGIRGV
Sbjct: 218 FLLYSLPDLFAQSLIHPLRIYLRSQSITLPLTFCAAFSILLHIPINYFLVSYLNFGIRGV 277

Query: 247 AMAGVWTNFNLVASLILYIVIFRVHQETWGGFSLECFKEWG 288
           A+AGVWTNFNLVASLILYI++FRVH++TWGGFSL+CFKEWG
Sbjct: 278 AIAGVWTNFNLVASLILYILVFRVHKDTWGGFSLQCFKEWG 316

BLAST of Lag0019984 vs. ExPASy TrEMBL
Match: A0A6J1JBI0 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111482957 PE=3 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 7.3e-114
Identity = 232/294 (78.91%), Postives = 252/294 (85.71%), Query Frame = 0

Query: 11  DSEIPYLNSINESL--------NLNLN----LTNPLIPQPPTSQLLTHKPTK----QTNT 70
           +SE+ YL+SIN+ L        N N N    LTNPLIPQ        HKPT      TNT
Sbjct: 9   NSEVAYLDSINDDLEKPSNSNSNSNSNSMHHLTNPLIPQQN-----RHKPTNNTNTNTNT 68

Query: 71  NTHFSLALHEAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDLPLAGGSLAIAFANIT 130
           NTHFSLA+ EAFSI +IAFPMVLTSLLLYSRSLISMLFLGRLG+LPLAGGSLAI FANIT
Sbjct: 69  NTHFSLAIKEAFSIFQIAFPMVLTSLLLYSRSLISMLFLGRLGELPLAGGSLAIGFANIT 128

Query: 131 GYSILSGLAIGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISILWLNVEPILVLC 190
           GYSILSGLA+GMEPIC QAFGAK+F+LLGLALQRTILLL+  SIPIS+LW NV+ IL+LC
Sbjct: 129 GYSILSGLALGMEPICGQAFGAKRFNLLGLALQRTILLLTFASIPISLLWFNVKNILLLC 188

Query: 191 KQDRSIASQAQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATFSILLHIPINYF 250
           KQD SIASQAQLFLLYSVPDL AQSLIHPLRIYLR+QSITLPLTFCATFSILLHIPINYF
Sbjct: 189 KQDASIASQAQLFLLYSVPDLFAQSLIHPLRIYLRTQSITLPLTFCATFSILLHIPINYF 248

Query: 251 LVSYLDFGIRGVAMAGVWTNFNLVASLILYIVIFRVHQETWGGFSLECFKEWGS 289
           LVSYL+ GIRGVA+AGVWTNFNLVASLILYI+IF VH+ETWGGFSLECFKEWG+
Sbjct: 249 LVSYLNLGIRGVAIAGVWTNFNLVASLILYILIFNVHKETWGGFSLECFKEWGN 297

BLAST of Lag0019984 vs. TAIR 10
Match: AT4G23030.1 (MATE efflux family protein )

HSP 1 Score: 303.1 bits (775), Expect = 2.5e-82
Identity = 155/234 (66.24%), Postives = 195/234 (83.33%), Query Frame = 0

Query: 56  THFSLALHEAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDL-PLAGGSLAIAFANIT 115
           TH S ++ EA SI++I+ P++LT LLLYSRS+ISMLFLGRL DL  L+GGSLA+ FANIT
Sbjct: 24  THLSSSIQEAKSIAKISLPLILTGLLLYSRSMISMLFLGRLNDLSALSGGSLALGFANIT 83

Query: 116 GYSILSGLAIGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISILWLNVEPILVLC 175
           GYS+LSGL+IGMEPIC QAFGAK+F LLGLALQRT LLL L S+PISILWLN++ IL+  
Sbjct: 84  GYSLLSGLSIGMEPICVQAFGAKRFKLLGLALQRTTLLLLLCSLPISILWLNIKKILLFF 143

Query: 176 KQDRSIASQAQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATFSILLHIPINYF 235
            QD  I++QA++F+L+S+PDL+ QS +HP+RIYLRSQSITLPLT+ A F++LLHIPINY 
Sbjct: 144 GQDEEISNQAEIFILFSLPDLILQSFLHPIRIYLRSQSITLPLTYSAFFAVLLHIPINYL 203

Query: 236 LVSYLDFGIRGVAMAGVWTNFNLVASLILYIVIFRVHQETWGGFSLECFKEWGS 289
           LVS L  G++GVA+  +WTN NL+  LI+YIV   V+Q+TWGGFS++CFK W S
Sbjct: 204 LVSSLGLGLKGVALGAIWTNVNLLGFLIIYIVFSGVYQKTWGGFSMDCFKGWRS 257

BLAST of Lag0019984 vs. TAIR 10
Match: AT1G58340.1 (MATE efflux family protein )

HSP 1 Score: 245.4 bits (625), Expect = 6.2e-65
Identity = 128/231 (55.41%), Postives = 169/231 (73.16%), Query Frame = 0

Query: 58  FSLALHEAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDLPLAGGSLAIAFANITGYS 117
           F   L E  +I +I+ P  +T LL+YSR++ISMLFLG LG+L LAGGSL+I FANITGYS
Sbjct: 52  FLEGLEEVKAIGKISGPTAMTGLLMYSRAMISMLFLGYLGELELAGGSLSIGFANITGYS 111

Query: 118 ILSGLAIGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISILWLNVEPILVLCKQD 177
           ++SGL++GMEPIC QA+GAK+  LLGL LQRT+LLL   S+PIS  WLN+  IL+ C QD
Sbjct: 112 VISGLSMGMEPICGQAYGAKQMKLLGLTLQRTVLLLLSCSVPISFSWLNMRRILLWCGQD 171

Query: 178 RSIASQAQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATFSILLHIPINYFLVS 237
             I+S AQ FLL+++PDL   SL+HPLRIYLR+Q+ITLP+T+    S+LLH+P+NY LV 
Sbjct: 172 EEISSVAQQFLLFAIPDLFLLSLLHPLRIYLRTQNITLPVTYSTAVSVLLHVPLNYLLVV 231

Query: 238 YLDFGIRGVAMAGVWTNFNLVASLILYIVIFRVHQETWGGFSLECFKEWGS 289
            L+ G+ GVA+A V TN NLV  L  ++    VH +TW   +++  K W +
Sbjct: 232 KLEMGVAGVAIAMVLTNLNLVVLLSSFVYFTSVHSDTWVPITIDSLKGWSA 282

BLAST of Lag0019984 vs. TAIR 10
Match: AT5G52050.1 (MATE efflux family protein )

HSP 1 Score: 243.0 bits (619), Expect = 3.1e-64
Identity = 125/225 (55.56%), Postives = 175/225 (77.78%), Query Frame = 0

Query: 44  LTHKPTKQTNTNTHFSLALHEAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDLPLAG 103
           L  K +   N ++  S+ L+EA SI +I++P+VLT L LY RS +S+ FLG LGD  LAG
Sbjct: 15  LLQKTSHLKNHSSVLSVFLNEAISICKISYPLVLTGLFLYVRSFVSLSFLGGLGDATLAG 74

Query: 104 GSLAIAFANITGYSILSGLAIGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISIL 163
           GSLA AFANITGYS+ SGL +G+E IC+QAFGA++++ +  +++R I+LL + S+P+++L
Sbjct: 75  GSLAAAFANITGYSLFSGLTMGVESICSQAFGARRYNYVCASVKRGIILLLVTSLPVTLL 134

Query: 164 WLNVEPILVLCKQDRSIASQAQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATF 223
           W+N+E IL++ KQD+ +AS+A +FLLYSVPDL+AQS +HPLR+YLR+QS TLPL+ C   
Sbjct: 135 WMNMEKILLILKQDKKLASEAHIFLLYSVPDLVAQSFLHPLRVYLRTQSKTLPLSICTVI 194

Query: 224 SILLHIPINYFLVSYLDFGIRGVAMAGVWTNFNLVASLILYIVIF 269
           +  LH+PI +FLVSYL  GI+G+A++GV +NFNLVA L LYI  F
Sbjct: 195 ASFLHLPITFFLVSYLGLGIKGIALSGVVSNFNLVAFLFLYICFF 239

BLAST of Lag0019984 vs. TAIR 10
Match: AT5G19700.1 (MATE efflux family protein )

HSP 1 Score: 235.7 bits (600), Expect = 4.9e-62
Identity = 125/224 (55.80%), Postives = 163/224 (72.77%), Query Frame = 0

Query: 64  EAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDLPLAGGSLAIAFANITGYSILSGLA 123
           EA S+  +AFP +L +L+LY+RS ISMLFLG +G+L LAGGSLAIAFANITGYS+L+GLA
Sbjct: 36  EARSLFSLAFPTILAALILYARSAISMLFLGHIGELELAGGSLAIAFANITGYSVLAGLA 95

Query: 124 IGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISILWLNVEPILVLCKQDRSIASQ 183
           +GM+P+C+QAFGA +  LL L LQRT+L L   S+ I  LWLN+  I++   QD SI+S 
Sbjct: 96  LGMDPLCSQAFGAGRPKLLSLTLQRTVLFLLTSSVVIVALWLNLGKIMIYLHQDPSISSL 155

Query: 184 AQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATFSILLHIPINYFLVSYLDFGI 243
           AQ ++L S+PDLL  S +HPLRIYLR+Q IT PLT       + HIP+N+FLVSYL +G 
Sbjct: 156 AQTYILCSIPDLLTNSFLHPLRIYLRAQGITSPLTLATLAGTIFHIPMNFFLVSYLGWGF 215

Query: 244 RGVAMAGVWTNFNLVASLILYIVIFRVHQETWGGFSLECFKEWG 288
            GV+MA   +N  +V  L+ ++ I  +HQ TW   S ECFK+WG
Sbjct: 216 MGVSMAAAASNLLVVIFLVAHVWIAGLHQPTWTRPSSECFKDWG 259

BLAST of Lag0019984 vs. TAIR 10
Match: AT4G29140.1 (MATE efflux family protein )

HSP 1 Score: 227.6 bits (579), Expect = 1.3e-59
Identity = 120/226 (53.10%), Postives = 160/226 (70.80%), Query Frame = 0

Query: 61  ALHEAFSISRIAFPMVLTSLLLYSRSLISMLFLGRLGDLPLAGGSLAIAFANITGYSILS 120
           A+ EA S+  +AFP+ +T+L+LY RS +SM FLG+LGDL LA GSLAIAFANITGYS+LS
Sbjct: 53  AVTEAKSLFTLAFPIAVTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAFANITGYSVLS 112

Query: 121 GLAIGMEPICAQAFGAKKFSLLGLALQRTILLLSLVSIPISILWLNVEPILVLCKQDRSI 180
           GLA+GMEP+C+QAFGA +F LL L L RT++ L +  +PIS+LW NV  I V   QD  I
Sbjct: 113 GLALGMEPLCSQAFGAHRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKISVYLHQDPDI 172

Query: 181 ASQAQLFLLYSVPDLLAQSLIHPLRIYLRSQSITLPLTFCATFSILLHIPINYFLVSYLD 240
           A  AQ +L++S+PDLL  +L+HP+RIYLR+Q I  P+T  +    + H+P N FLVSYL 
Sbjct: 173 AKLAQTYLIFSLPDLLTNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLPANLFLVSYLR 232

Query: 241 FGIRGVAMAGVWTNFNLVASLILYIVIFRVHQETWGGFSLECFKEW 287
            G+ GVA+A   TN  +VA L+ Y+    +H  TW   + +CF+ W
Sbjct: 233 LGLTGVAVASSITNIFVVAFLVCYVWASGLHAPTWTDPTRDCFRGW 278

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6574153.11.4e-12483.28Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. sororia] >KAG7... [more]
XP_023542378.12.5e-12482.94protein DETOXIFICATION 49-like [Cucurbita pepo subsp. pepo][more]
XP_022944944.13.2e-12482.94protein DETOXIFICATION 49-like [Cucurbita moschata][more]
XP_022968021.13.6e-12382.59protein DETOXIFICATION 49-like [Cucurbita maxima][more]
XP_038880058.11.0e-12281.94protein DETOXIFICATION 49 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
O827523.5e-8166.24Protein DETOXIFICATION 49 OS=Arabidopsis thaliana OX=3702 GN=DTX49 PE=2 SV=1[more]
Q9SLV08.7e-6455.41Protein DETOXIFICATION 48 OS=Arabidopsis thaliana OX=3702 GN=DTX48 PE=2 SV=1[more]
Q9FJ874.3e-6355.56Protein DETOXIFICATION 50 OS=Arabidopsis thaliana OX=3702 GN=DTX50 PE=2 SV=1[more]
Q4PSF46.9e-6155.80Protein DETOXIFICATION 52 OS=Arabidopsis thaliana OX=3702 GN=DTX52 PE=2 SV=1[more]
Q9SZE21.9e-5853.10Protein DETOXIFICATION 51 OS=Arabidopsis thaliana OX=3702 GN=DTX51 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FZK11.6e-12482.94Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111449327 PE=3 SV=1[more]
A0A6J1HTP91.7e-12382.59Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111467391 PE=3 SV=1[more]
A0A6J1E4L71.0e-11580.84Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111430734 PE=3 SV=1[more]
A0A0A0L6E55.6e-11481.14Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_3G180310 PE=3 SV=1[more]
A0A6J1JBI07.3e-11478.91Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111482957 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G23030.12.5e-8266.24MATE efflux family protein [more]
AT1G58340.16.2e-6555.41MATE efflux family protein [more]
AT5G52050.13.1e-6455.56MATE efflux family protein [more]
AT5G19700.14.9e-6255.80MATE efflux family protein [more]
AT4G29140.11.3e-5953.10MATE efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002528Multi antimicrobial extrusion proteinPFAMPF01554MatEcoord: 74..234
e-value: 2.7E-37
score: 127.9
NoneNo IPR availablePANTHERPTHR11206:SF394PROTEIN DETOXIFICATIONcoord: 43..289
NoneNo IPR availablePANTHERPTHR11206MULTIDRUG RESISTANCE PROTEINcoord: 43..289

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0019984.1Lag0019984.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0042910 xenobiotic transmembrane transporter activity