Homology
BLAST of Lag0019961 vs. NCBI nr
Match:
CAA70329.1 (gibberellin 20-oxidase [Marah macrocarpa])
HSP 1 Score: 625.5 bits (1612), Expect = 2.9e-175
Identity = 295/371 (79.51%), Postives = 329/371 (88.68%), Query Frame = 0
Query: 1 MAIKCMTATRSSPSAPGESKREHHLTFGSSGESKVPEDFIWPDEFKASEPVPELPVPHID 60
MAI CM AT SSP+ E KREH + FG +GES VPE+FIWPDEFKA+ PEL VPHID
Sbjct: 1 MAITCMMATESSPAE--EVKREHQVAFGGAGESSVPENFIWPDEFKATADAPELHVPHID 60
Query: 61 MKKFLSGNESDVEEAARVVDKACRKHGFFVVVNHGVDMNMMKDVHECMDEFFSLPLEVKE 120
+KK LSG+E DVEEA R+VD+ACRKHGFFVVVNHGVD +M VHECM+EFF+LPL+VK+
Sbjct: 61 LKKVLSGDEKDVEEATRLVDEACRKHGFFVVVNHGVDKELMNKVHECMNEFFTLPLDVKQ 120
Query: 121 KAHRKVGENFGYANSFIGRFSNKLPWKETFSVRSLADENLPTAYNYVCEVLGQEFSHHGK 180
KAHRKVGENFGYANSFIGRFS KLPWKETFS+R LA EN TA +YV +VLG EFSHHG+
Sbjct: 121 KAHRKVGENFGYANSFIGRFSTKLPWKETFSLRYLAHENSSTARDYVSQVLGPEFSHHGE 180
Query: 181 VYQEFGKAMSDLGLKIVELLGLSLGIQREHYKKFYEDNDSIIRLNYYPPCEKPELTLGTG 240
VYQE GKA+SDL L+IVELLGLSLGI RE ++KFYEDNDSI+R+NYYP CEKPELTLGTG
Sbjct: 181 VYQECGKALSDLSLRIVELLGLSLGISRETFRKFYEDNDSIMRMNYYPRCEKPELTLGTG 240
Query: 241 PHCDPTSITILHQDHVSGLQVYVDDQWYAIPPTPDSFVVNIGDTFMSLTNGVYKSCFHRA 300
PHCDPTSITILHQD VSGLQVYVDDQW++IPPT DSFVVN+GDTFMSLTNGVYKSCFHRA
Sbjct: 241 PHCDPTSITILHQDDVSGLQVYVDDQWHSIPPTEDSFVVNVGDTFMSLTNGVYKSCFHRA 300
Query: 301 VVNPKETRKSVAFFLCPAADKLVRAPEELVEKNPPRKFPDYTWPMLLEMTQKFYRADSNT 360
VVN KE RKS+AFFLCPA +K+VRAPEELVEK PPRKFPDYTWPMLLEMTQK+YRADSNT
Sbjct: 301 VVNCKEARKSMAFFLCPAVEKVVRAPEELVEKYPPRKFPDYTWPMLLEMTQKYYRADSNT 360
Query: 361 FKAFTSWLQEQ 372
F AFT+W+Q+Q
Sbjct: 361 FNAFTTWIQQQ 369
BLAST of Lag0019961 vs. NCBI nr
Match:
XP_022984437.1 (gibberellin 20 oxidase 2-like [Cucurbita maxima])
HSP 1 Score: 624.4 bits (1609), Expect = 6.5e-175
Identity = 292/387 (75.45%), Postives = 337/387 (87.08%), Query Frame = 0
Query: 1 MAIKCMTATRSSPSA--PGESKREHHLTFGSSGESKVPEDFIWPDEFKASEPVPELPVPH 60
MAIKCMT +S + E KR+H + FGSS ES VPE+FIWPDEFKA E VPEL VPH
Sbjct: 1 MAIKCMTTAKSQGESKLKQEMKRKHRVEFGSSDESMVPENFIWPDEFKARESVPELDVPH 60
Query: 61 IDMKKFLSGNESDVEEAARVVDKACRKHGFFVVVNHGVDMNMMKDVHECMDEFFSLPLEV 120
ID++KF+S NE+D+EEA R+VD+ACRKHGFFV+VNHGVDM +MK+VHECMDEFFSLPL+V
Sbjct: 61 IDLQKFVSENENDIEEATRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLPLDV 120
Query: 121 KEKAHRKVGENFGYANSFIGRFSNKLPWKETFSVRSLADENLPTAYNYVCEVLGQEFSHH 180
K+KA RKVGEN+GYANSFIGRFS+KLPWKETFS+R A NLP ++YVC LG EFS
Sbjct: 121 KQKAQRKVGENYGYANSFIGRFSSKLPWKETFSLRYAAHHNLPVTHDYVCHHLGPEFSQQ 180
Query: 181 GKVYQEFGKAMSDLGLKIVELLGLSLGIQREHYKKFYEDNDSIIRLNYYPPCEKPELTLG 240
GKVY+E GKA+SDL LKIVELLGLSLG+ +E +KKF+EDNDSIIRLNYYPPC KPELTLG
Sbjct: 181 GKVYEECGKALSDLSLKIVELLGLSLGVPKEKFKKFFEDNDSIIRLNYYPPCNKPELTLG 240
Query: 241 TGPHCDPTSITILHQDHVSGLQVYVDDQWYAIPPTPDSFVVNIGDTFMSLTNGVYKSCFH 300
TGPHCDPTSITILHQDHVSGLQVYVDDQW++IPP DSFV+NIGDTFM+LTNGVYKSCFH
Sbjct: 241 TGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYKSCFH 300
Query: 301 RAVVNPKETRKSVAFFLCPAADKLVRAPEELVEKNPPRKFPDYTWPMLLEMTQKFYRADS 360
RAVVN +E RKS+ FFLCPA DK+VRAP+E+V+KNPPRKFPDYTWPMLLE+TQKFYR+DS
Sbjct: 301 RAVVNCREARKSIVFFLCPADDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFYRSDS 360
Query: 361 NTFKAFTSWLQEQKKLEGGSCTVAPSV 386
NTFKAFT+WLQ K L + ++APS+
Sbjct: 361 NTFKAFTTWLQ-HKNLATAASSLAPSL 386
BLAST of Lag0019961 vs. NCBI nr
Match:
CBL59326.1 (Gibberellin 20-oxidase [Cucurbita maxima])
HSP 1 Score: 622.9 bits (1605), Expect = 1.9e-174
Identity = 291/387 (75.19%), Postives = 337/387 (87.08%), Query Frame = 0
Query: 1 MAIKCMTATRSSPSA--PGESKREHHLTFGSSGESKVPEDFIWPDEFKASEPVPELPVPH 60
MAIKCMT +S + E KR+H + FGSS ES VPE+FIWPDEFKA E VPEL VPH
Sbjct: 1 MAIKCMTTAKSQGESKLKQEMKRKHRVEFGSSDESMVPENFIWPDEFKARESVPELDVPH 60
Query: 61 IDMKKFLSGNESDVEEAARVVDKACRKHGFFVVVNHGVDMNMMKDVHECMDEFFSLPLEV 120
ID++KF+S NE+D+EEA R+VD+ACRKHGFFV+VNHGVDM +MK+VHECMDEFFSLPL+V
Sbjct: 61 IDLQKFVSENENDIEEATRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLPLDV 120
Query: 121 KEKAHRKVGENFGYANSFIGRFSNKLPWKETFSVRSLADENLPTAYNYVCEVLGQEFSHH 180
K+KA RKVGEN+GYANSFIGRFS+KLPWKETFS+R A NLP ++YVC LG EFS
Sbjct: 121 KQKAQRKVGENYGYANSFIGRFSSKLPWKETFSLRYAAHHNLPVTHDYVCHHLGPEFSQQ 180
Query: 181 GKVYQEFGKAMSDLGLKIVELLGLSLGIQREHYKKFYEDNDSIIRLNYYPPCEKPELTLG 240
GKVY+E G+A+SDL LKIVELLGLSLG+ +E +KKF+EDNDSIIRLNYYPPC KPELTLG
Sbjct: 181 GKVYEECGEALSDLSLKIVELLGLSLGVPKEKFKKFFEDNDSIIRLNYYPPCNKPELTLG 240
Query: 241 TGPHCDPTSITILHQDHVSGLQVYVDDQWYAIPPTPDSFVVNIGDTFMSLTNGVYKSCFH 300
TGPHCDPTSITILHQDHVSGLQVYVDDQW++IPP DSFV+NIGDTFM+LTNGVYKSCFH
Sbjct: 241 TGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYKSCFH 300
Query: 301 RAVVNPKETRKSVAFFLCPAADKLVRAPEELVEKNPPRKFPDYTWPMLLEMTQKFYRADS 360
RAVVN +E RKS+ FFLCPA DK+VRAP+E+V+KNPPRKFPDYTWPMLLE+TQKFYR+DS
Sbjct: 301 RAVVNCREARKSIVFFLCPADDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFYRSDS 360
Query: 361 NTFKAFTSWLQEQKKLEGGSCTVAPSV 386
NTFKAFT+WLQ K L + ++APS+
Sbjct: 361 NTFKAFTTWLQ-HKNLATAASSLAPSL 386
BLAST of Lag0019961 vs. NCBI nr
Match:
XP_023552793.1 (gibberellin 20 oxidase 2-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 620.2 bits (1598), Expect = 1.2e-173
Identity = 289/387 (74.68%), Postives = 336/387 (86.82%), Query Frame = 0
Query: 1 MAIKCMTATRSSPSA--PGESKREHHLTFGSSGESKVPEDFIWPDEFKASEPVPELPVPH 60
MAIKCMT +S + E KR+H + FGSS ES VPE+FIWP+EFKA E VPEL VPH
Sbjct: 1 MAIKCMTTAKSQGESKLKQEMKRKHRVEFGSSDESMVPENFIWPEEFKARESVPELQVPH 60
Query: 61 IDMKKFLSGNESDVEEAARVVDKACRKHGFFVVVNHGVDMNMMKDVHECMDEFFSLPLEV 120
ID++KF+S NE+D+EE R+VD+ACRKHGFFV+VNHGVDM +MK+VHECMDEFFSLPL+V
Sbjct: 61 IDLQKFMSENENDIEEVRRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLPLDV 120
Query: 121 KEKAHRKVGENFGYANSFIGRFSNKLPWKETFSVRSLADENLPTAYNYVCEVLGQEFSHH 180
K+KA RKVGEN+GYANSFIGRFS+KLPWKETFS+R A N P ++YVC LGQEFS
Sbjct: 121 KQKAQRKVGENYGYANSFIGRFSSKLPWKETFSLRYAAHHNFPITHDYVCHHLGQEFSQQ 180
Query: 181 GKVYQEFGKAMSDLGLKIVELLGLSLGIQREHYKKFYEDNDSIIRLNYYPPCEKPELTLG 240
GKVY+E GKA+SDL LKIVELLGLSLG+ ++ +KKF+EDNDSIIRLNYYPPC KPELTLG
Sbjct: 181 GKVYEECGKALSDLSLKIVELLGLSLGLPKQKFKKFFEDNDSIIRLNYYPPCNKPELTLG 240
Query: 241 TGPHCDPTSITILHQDHVSGLQVYVDDQWYAIPPTPDSFVVNIGDTFMSLTNGVYKSCFH 300
TGPHCDPTSITILHQDHVSGLQVYVDDQW++IPP DSFV+NIGDTFM+LTNGVYKSCFH
Sbjct: 241 TGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYKSCFH 300
Query: 301 RAVVNPKETRKSVAFFLCPAADKLVRAPEELVEKNPPRKFPDYTWPMLLEMTQKFYRADS 360
RAVVN +E RKS+ FFLCPA DK+VRAP+E+V+KNPPRKFPDYTWPMLLE+TQKFYR+DS
Sbjct: 301 RAVVNCREARKSIVFFLCPAGDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFYRSDS 360
Query: 361 NTFKAFTSWLQEQKKLEGGSCTVAPSV 386
NTFKAFT+WLQ K L + ++APS+
Sbjct: 361 NTFKAFTTWLQ-HKNLATTASSLAPSL 386
BLAST of Lag0019961 vs. NCBI nr
Match:
XP_022922616.1 (gibberellin 20 oxidase 2-like [Cucurbita moschata] >KAG6576758.1 Gibberellin 20 oxidase 2, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 615.5 bits (1586), Expect = 3.0e-172
Identity = 289/387 (74.68%), Postives = 336/387 (86.82%), Query Frame = 0
Query: 1 MAIKCMTATRSSPSA--PGESKREHHLTFGSSGESKVPEDFIWPDEFKASEPVPELPVPH 60
MAIKCMT +S + E KR+H + FGSS ES VPE+FIWP+EFKA E VPEL VPH
Sbjct: 1 MAIKCMTTAKSQGESKLKQEMKRKHRVEFGSSDESMVPENFIWPEEFKARESVPELHVPH 60
Query: 61 IDMKKFLSGNESDVEEAARVVDKACRKHGFFVVVNHGVDMNMMKDVHECMDEFFSLPLEV 120
ID++KF+S NE+D+EE R+VD+ACRKHGFFV+VNHGVDM +MK+VHECMDEFFSLPL+V
Sbjct: 61 IDLQKFMSENENDIEEVRRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLPLDV 120
Query: 121 KEKAHRKVGENFGYANSFIGRFSNKLPWKETFSVRSLADENLPTAYNYVCEVLGQEFSHH 180
K+KA RKVGEN+GYANSFIGRFS+KLPWKETFS+R A P ++YVC LGQEFS
Sbjct: 121 KQKAQRKVGENYGYANSFIGRFSSKLPWKETFSLRYAAHH--PITHDYVCHHLGQEFSQQ 180
Query: 181 GKVYQEFGKAMSDLGLKIVELLGLSLGIQREHYKKFYEDNDSIIRLNYYPPCEKPELTLG 240
GKVY+E GKA+SDL LKIVELLGLSLG+ ++ +KKF+EDNDSIIRLNYYPPC KPELTLG
Sbjct: 181 GKVYEECGKALSDLSLKIVELLGLSLGVPKQKFKKFFEDNDSIIRLNYYPPCNKPELTLG 240
Query: 241 TGPHCDPTSITILHQDHVSGLQVYVDDQWYAIPPTPDSFVVNIGDTFMSLTNGVYKSCFH 300
TGPHCDPTSITILHQDHVSGLQVYVDDQW++IPP DSFV+NIGDTFM+LTNGVYKSCFH
Sbjct: 241 TGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYKSCFH 300
Query: 301 RAVVNPKETRKSVAFFLCPAADKLVRAPEELVEKNPPRKFPDYTWPMLLEMTQKFYRADS 360
RAVVN +E RKS+ FFLCPA DK+VRAP+E+V+KNPPRKFPDYTWPMLLE+TQKFYR+DS
Sbjct: 301 RAVVNCREARKSIVFFLCPAGDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFYRSDS 360
Query: 361 NTFKAFTSWLQEQKKLEGGSCTVAPSV 386
NTFKAFT+WLQ KKL + ++APS+
Sbjct: 361 NTFKAFTTWLQ-HKKLATAASSLAPSL 384
BLAST of Lag0019961 vs. ExPASy Swiss-Prot
Match:
Q39111 (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1)
HSP 1 Score: 429.9 bits (1104), Expect = 3.1e-119
Identity = 209/376 (55.59%), Postives = 271/376 (72.07%), Query Frame = 0
Query: 1 MAIKCMTAT----RSSPSAPGESKREHHLTFGSS---GESKVPEDFIWPDEFKASEPVPE 60
MAI C T + P E + L F S +S++P FIWPDE K S +PE
Sbjct: 1 MAILCTTTSPAEKEHEPKQDLEKDQTSPLIFNPSLLNLQSQIPNQFIWPDEEKPSIDIPE 60
Query: 61 LPVPHIDMKKFLSGNESDVEEAARVVDKACRKHGFFVVVNHGVDMNMMKDVHECMDEFFS 120
L VP ID LS +S + EA RV+ +AC KHGFF+VVNHGV +++ D H M+ FF
Sbjct: 61 LNVPFID----LSSQDSTL-EAPRVIAEACTKHGFFLVVNHGVSESLIADAHRLMESFFD 120
Query: 121 LPLEVKEKAHRKVGENFGYANSFIGRFSNKLPWKETFSVRSLADEN-LPTAYNYVCEVLG 180
+PL K+KA RK GE+ GYA+SF GRFS KLPWKET S + D + T +Y + LG
Sbjct: 121 MPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSNDNSGSRTVQDYFSDTLG 180
Query: 181 QEFSHHGKVYQEFGKAMSDLGLKIVELLGLSLGIQREHYKKFYEDNDSIIRLNYYPPCEK 240
QEF GKVYQ++ +AMS L LKI+ELLGLSLG+ R++++ F+E+NDSI+RLN+YPPC+
Sbjct: 181 QEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQT 240
Query: 241 PELTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWYAIPPTPDSFVVNIGDTFMSLTNGV 300
P+LTLGTGPHCDP+S+TILHQDHV+GLQV+VD+QW +I P P +FVVNIGDTFM+L+NG+
Sbjct: 241 PDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNGI 300
Query: 301 YKSCFHRAVVNPKETRKSVAFFLCPAADKLVRAPEELVEKNPPRKFPDYTWPMLLEMTQK 360
+KSC HRAVVN + RKS+AFFLCP DK+V+ P +++EK RK+PD+TW M LE TQK
Sbjct: 301 FKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQK 360
Query: 361 FYRADSNTFKAFTSWL 369
YRAD NT +F++W+
Sbjct: 361 HYRADVNTLDSFSNWV 371
BLAST of Lag0019961 vs. ExPASy Swiss-Prot
Match:
Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)
HSP 1 Score: 421.4 bits (1082), Expect = 1.1e-116
Identity = 194/338 (57.40%), Postives = 253/338 (74.85%), Query Frame = 0
Query: 32 ESKVPEDFIWPDEFKASEPVPELPVPHIDMKKFLSGNESDVEEAARVVDKACRKHGFFVV 91
++ +P FIWPD+ K S V EL VP ID++ LS + S +A+R++ +AC+KHGFF+V
Sbjct: 37 QANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLV 96
Query: 92 VNHGVDMNMMKDVHECMDEFFSLPLEVKEKAHRKVGENFGYANSFIGRFSNKLPWKETFS 151
VNHG+ ++ D HE FF +PL K++ RK GE+ GYA+SF GRFS KLPWKET S
Sbjct: 97 VNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLS 156
Query: 152 VRSLAD-ENLPTAYNYVCEVLGQEFSHHGKVYQEFGKAMSDLGLKIVELLGLSLGIQREH 211
R D + +Y C+ LG F GKVYQE+ +AMS L LKI+ELLGLSLG++R++
Sbjct: 157 FRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDY 216
Query: 212 YKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWYAI 271
+++F+E+NDSI+RLNYYPPC KP+LTLGTGPHCDPTS+TILHQDHV+GLQV+V++QW +I
Sbjct: 217 FREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSI 276
Query: 272 PPTPDSFVVNIGDTFMSLTNGVYKSCFHRAVVNPKETRKSVAFFLCPAADKLVRAPEELV 331
P P +FVVNIGDTFM+L+N YKSC HRAVVN + RKS+AFFLCP D++V P EL+
Sbjct: 277 RPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELL 336
Query: 332 EKNPPRKFPDYTWPMLLEMTQKFYRADSNTFKAFTSWL 369
+ R++PD+TW M LE TQK YRAD NT +AF+ WL
Sbjct: 337 DSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373
BLAST of Lag0019961 vs. ExPASy Swiss-Prot
Match:
Q39112 (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1)
HSP 1 Score: 415.2 bits (1066), Expect = 7.9e-115
Identity = 193/342 (56.43%), Postives = 251/342 (73.39%), Query Frame = 0
Query: 35 VPEDFIWPDEFKASEPVPELPVPHIDMKKFLSGNESDVEEAARVVDKACRKHGFFVVVNH 94
+P+ F+WPD K S V L VP ID+ FLSG+ EA R+V KA KHGFF++ NH
Sbjct: 37 IPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNH 96
Query: 95 GVDMNMMKDVHECMDEFFSLPLEVKEKAHRKVGENFGYANSFIGRFSNKLPWKETFSVRS 154
GVD +++ + MD FF P K+KA RK GE+ GYA+SF+GRFS+KLPWKET S +
Sbjct: 97 GVDESLLSRAYLHMDSFFKAPACEKQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKF 156
Query: 155 LADENL--PTAYNYVCEVLGQEFSHHGKVYQEFGKAMSDLGLKIVELLGLSLGIQREHYK 214
+E + T ++V + +G + GKVYQE+ +AM+ L LKI+ELLG+SLG++R ++K
Sbjct: 157 SPEEKIHSQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFK 216
Query: 215 KFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWYAIPP 274
+F+ED+DSI RLNYYP C++PEL LGTGPHCDPTS+TILHQD V GLQV+VD++W +IPP
Sbjct: 217 EFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPP 276
Query: 275 TPDSFVVNIGDTFMSLTNGVYKSCFHRAVVNPKETRKSVAFFLCPAADKLVRAPEELVE- 334
P +FVVNIGDTFM+LTNG YKSC HRAVVN + RK+ AFFLCP +K+V+ PEELV
Sbjct: 277 NPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNG 336
Query: 335 -KNPPRKFPDYTWPMLLEMTQKFYRADSNTFKAFTSWLQEQK 373
K+ RK+PD+TW M LE TQK YRAD NT F+ WL+ ++
Sbjct: 337 VKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSIWLKNRR 378
BLAST of Lag0019961 vs. ExPASy Swiss-Prot
Match:
Q9C955 (Gibberellin 20 oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=GA20OX4 PE=2 SV=1)
HSP 1 Score: 412.9 bits (1060), Expect = 3.9e-114
Identity = 192/341 (56.30%), Postives = 246/341 (72.14%), Query Frame = 0
Query: 35 VPEDFIWPDEFKASEPVPELPVPHIDMKKFLSGNESDVEEAARVVDKACRKHGFFVVVNH 94
+P++F+WPD K S+ VP L VP ID+ FLS + V EA R+V +A +KHGFF+V NH
Sbjct: 36 IPQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFLVTNH 95
Query: 95 GVDMNMMKDVHECMDEFFSLPLEVKEKAHRKVGENFGYANSFIGRFSNKLPWKET----F 154
GVD ++ H+ MD FF P K KA RKVGE GYA+SF+GRF LPWKET F
Sbjct: 96 GVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETLSFSF 155
Query: 155 SVRSLADENLPTAYNYVCEVLGQEFSHHGKVYQEFGKAMSDLGLKIVELLGLSLGIQREH 214
S ++ T NY+ + +G + G VYQE+ + MS+L LKI+ELLG+SLGI+REH
Sbjct: 156 SPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGIKREH 215
Query: 215 YKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWYAI 274
+++F+EDN+SI RLNYYP C++P+L LGTGPHCDPTS+TIL QD VSGLQV+VD+QW +I
Sbjct: 216 FREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQWQSI 275
Query: 275 PPTPDSFVVNIGDTFMSLTNGVYKSCFHRAVVNPKETRKSVAFFLCPAADKLVRAPEELV 334
PP P + VVNIGDT M+LTNG+YKSC HRAVVN + TRK++AFFLCP DK+V+ P EL
Sbjct: 276 PPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCPKVDKVVKPPSEL- 335
Query: 335 EKNPPRKFPDYTWPMLLEMTQKFYRADSNTFKAFTSWLQEQ 372
R +PD+TW M LE T K YRAD NT + FT+WL+ +
Sbjct: 336 --EGERAYPDFTWSMFLEFTMKHYRADMNTLEEFTNWLKNK 373
BLAST of Lag0019961 vs. ExPASy Swiss-Prot
Match:
O04707 (Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1)
HSP 1 Score: 394.8 bits (1013), Expect = 1.1e-108
Identity = 183/345 (53.04%), Postives = 246/345 (71.30%), Query Frame = 0
Query: 30 SGESKVPEDFIWPD-EFKASEPVPELPVPHIDMKKFLSGNESDVEEAARVVDKACRKHGF 89
SG S +P FIWP+ E + EL VP I++ LSG+ + E R+V +AC +HGF
Sbjct: 12 SGRSDIPSQFIWPEGESPTPDAAEELHVPLINIGGMLSGDAAAAAEVTRLVGEACERHGF 71
Query: 90 FVVVNHGVDMNMMKDVHECMDEFFSLPLEVKEKAHRKVGENFGYANSFIGRFSNKLPWKE 149
F VVNHG+D ++ D H C+D FF++PL K++A R+ GE+ GYA+SF GRF++KLPWKE
Sbjct: 72 FQVVNHGIDAELLADAHRCVDNFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKE 131
Query: 150 TFSVRSLADENLPTAYNYVCEVLGQEFSHHGKVYQEFGKAMSDLGLKIVELLGLSLGIQR 209
T S RS + +Y+ LG++ G+VY + MS L L+I+E+LG SLG+ R
Sbjct: 132 TLSFRSCPSDP-ALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGR 191
Query: 210 EHYKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWY 269
HY++F+E NDSI+RLNYYPPC++P TLGTGPHCDPTS+TILHQD+V GLQV+ + +W
Sbjct: 192 AHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWR 251
Query: 270 AIPPTPDSFVVNIGDTFMSLTNGVYKSCFHRAVVNPKETRKSVAFFLCPAADKLVRAPEE 329
+I P D+FVVNIGDTFM+L+NG YKSC HRAVVN + RKS+AFFLCP DK+V P
Sbjct: 252 SIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSRVPRKSLAFFLCPEMDKVVAPPGT 311
Query: 330 LVEKNPPRKFPDYTWPMLLEMTQKFYRADSNTFKAFTSWLQEQKK 374
LV+ + PR +PD+TW LL+ TQK YRAD T + F+SW+ +Q++
Sbjct: 312 LVDASNPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWIVQQQQ 355
BLAST of Lag0019961 vs. ExPASy TrEMBL
Match:
Q96498 (Gibberellin 20-oxidase OS=Marah macrocarpa OX=48242 PE=2 SV=1)
HSP 1 Score: 625.5 bits (1612), Expect = 1.4e-175
Identity = 295/371 (79.51%), Postives = 329/371 (88.68%), Query Frame = 0
Query: 1 MAIKCMTATRSSPSAPGESKREHHLTFGSSGESKVPEDFIWPDEFKASEPVPELPVPHID 60
MAI CM AT SSP+ E KREH + FG +GES VPE+FIWPDEFKA+ PEL VPHID
Sbjct: 1 MAITCMMATESSPAE--EVKREHQVAFGGAGESSVPENFIWPDEFKATADAPELHVPHID 60
Query: 61 MKKFLSGNESDVEEAARVVDKACRKHGFFVVVNHGVDMNMMKDVHECMDEFFSLPLEVKE 120
+KK LSG+E DVEEA R+VD+ACRKHGFFVVVNHGVD +M VHECM+EFF+LPL+VK+
Sbjct: 61 LKKVLSGDEKDVEEATRLVDEACRKHGFFVVVNHGVDKELMNKVHECMNEFFTLPLDVKQ 120
Query: 121 KAHRKVGENFGYANSFIGRFSNKLPWKETFSVRSLADENLPTAYNYVCEVLGQEFSHHGK 180
KAHRKVGENFGYANSFIGRFS KLPWKETFS+R LA EN TA +YV +VLG EFSHHG+
Sbjct: 121 KAHRKVGENFGYANSFIGRFSTKLPWKETFSLRYLAHENSSTARDYVSQVLGPEFSHHGE 180
Query: 181 VYQEFGKAMSDLGLKIVELLGLSLGIQREHYKKFYEDNDSIIRLNYYPPCEKPELTLGTG 240
VYQE GKA+SDL L+IVELLGLSLGI RE ++KFYEDNDSI+R+NYYP CEKPELTLGTG
Sbjct: 181 VYQECGKALSDLSLRIVELLGLSLGISRETFRKFYEDNDSIMRMNYYPRCEKPELTLGTG 240
Query: 241 PHCDPTSITILHQDHVSGLQVYVDDQWYAIPPTPDSFVVNIGDTFMSLTNGVYKSCFHRA 300
PHCDPTSITILHQD VSGLQVYVDDQW++IPPT DSFVVN+GDTFMSLTNGVYKSCFHRA
Sbjct: 241 PHCDPTSITILHQDDVSGLQVYVDDQWHSIPPTEDSFVVNVGDTFMSLTNGVYKSCFHRA 300
Query: 301 VVNPKETRKSVAFFLCPAADKLVRAPEELVEKNPPRKFPDYTWPMLLEMTQKFYRADSNT 360
VVN KE RKS+AFFLCPA +K+VRAPEELVEK PPRKFPDYTWPMLLEMTQK+YRADSNT
Sbjct: 301 VVNCKEARKSMAFFLCPAVEKVVRAPEELVEKYPPRKFPDYTWPMLLEMTQKYYRADSNT 360
Query: 361 FKAFTSWLQEQ 372
F AFT+W+Q+Q
Sbjct: 361 FNAFTTWIQQQ 369
BLAST of Lag0019961 vs. ExPASy TrEMBL
Match:
A0A6J1J263 (gibberellin 20 oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482738 PE=3 SV=1)
HSP 1 Score: 624.4 bits (1609), Expect = 3.2e-175
Identity = 292/387 (75.45%), Postives = 337/387 (87.08%), Query Frame = 0
Query: 1 MAIKCMTATRSSPSA--PGESKREHHLTFGSSGESKVPEDFIWPDEFKASEPVPELPVPH 60
MAIKCMT +S + E KR+H + FGSS ES VPE+FIWPDEFKA E VPEL VPH
Sbjct: 1 MAIKCMTTAKSQGESKLKQEMKRKHRVEFGSSDESMVPENFIWPDEFKARESVPELDVPH 60
Query: 61 IDMKKFLSGNESDVEEAARVVDKACRKHGFFVVVNHGVDMNMMKDVHECMDEFFSLPLEV 120
ID++KF+S NE+D+EEA R+VD+ACRKHGFFV+VNHGVDM +MK+VHECMDEFFSLPL+V
Sbjct: 61 IDLQKFVSENENDIEEATRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLPLDV 120
Query: 121 KEKAHRKVGENFGYANSFIGRFSNKLPWKETFSVRSLADENLPTAYNYVCEVLGQEFSHH 180
K+KA RKVGEN+GYANSFIGRFS+KLPWKETFS+R A NLP ++YVC LG EFS
Sbjct: 121 KQKAQRKVGENYGYANSFIGRFSSKLPWKETFSLRYAAHHNLPVTHDYVCHHLGPEFSQQ 180
Query: 181 GKVYQEFGKAMSDLGLKIVELLGLSLGIQREHYKKFYEDNDSIIRLNYYPPCEKPELTLG 240
GKVY+E GKA+SDL LKIVELLGLSLG+ +E +KKF+EDNDSIIRLNYYPPC KPELTLG
Sbjct: 181 GKVYEECGKALSDLSLKIVELLGLSLGVPKEKFKKFFEDNDSIIRLNYYPPCNKPELTLG 240
Query: 241 TGPHCDPTSITILHQDHVSGLQVYVDDQWYAIPPTPDSFVVNIGDTFMSLTNGVYKSCFH 300
TGPHCDPTSITILHQDHVSGLQVYVDDQW++IPP DSFV+NIGDTFM+LTNGVYKSCFH
Sbjct: 241 TGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYKSCFH 300
Query: 301 RAVVNPKETRKSVAFFLCPAADKLVRAPEELVEKNPPRKFPDYTWPMLLEMTQKFYRADS 360
RAVVN +E RKS+ FFLCPA DK+VRAP+E+V+KNPPRKFPDYTWPMLLE+TQKFYR+DS
Sbjct: 301 RAVVNCREARKSIVFFLCPADDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFYRSDS 360
Query: 361 NTFKAFTSWLQEQKKLEGGSCTVAPSV 386
NTFKAFT+WLQ K L + ++APS+
Sbjct: 361 NTFKAFTTWLQ-HKNLATAASSLAPSL 386
BLAST of Lag0019961 vs. ExPASy TrEMBL
Match:
I0J1I7 (Gibberellin 20-oxidase OS=Cucurbita maxima OX=3661 GN=ga20ox4 PE=2 SV=1)
HSP 1 Score: 622.9 bits (1605), Expect = 9.2e-175
Identity = 291/387 (75.19%), Postives = 337/387 (87.08%), Query Frame = 0
Query: 1 MAIKCMTATRSSPSA--PGESKREHHLTFGSSGESKVPEDFIWPDEFKASEPVPELPVPH 60
MAIKCMT +S + E KR+H + FGSS ES VPE+FIWPDEFKA E VPEL VPH
Sbjct: 1 MAIKCMTTAKSQGESKLKQEMKRKHRVEFGSSDESMVPENFIWPDEFKARESVPELDVPH 60
Query: 61 IDMKKFLSGNESDVEEAARVVDKACRKHGFFVVVNHGVDMNMMKDVHECMDEFFSLPLEV 120
ID++KF+S NE+D+EEA R+VD+ACRKHGFFV+VNHGVDM +MK+VHECMDEFFSLPL+V
Sbjct: 61 IDLQKFVSENENDIEEATRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLPLDV 120
Query: 121 KEKAHRKVGENFGYANSFIGRFSNKLPWKETFSVRSLADENLPTAYNYVCEVLGQEFSHH 180
K+KA RKVGEN+GYANSFIGRFS+KLPWKETFS+R A NLP ++YVC LG EFS
Sbjct: 121 KQKAQRKVGENYGYANSFIGRFSSKLPWKETFSLRYAAHHNLPVTHDYVCHHLGPEFSQQ 180
Query: 181 GKVYQEFGKAMSDLGLKIVELLGLSLGIQREHYKKFYEDNDSIIRLNYYPPCEKPELTLG 240
GKVY+E G+A+SDL LKIVELLGLSLG+ +E +KKF+EDNDSIIRLNYYPPC KPELTLG
Sbjct: 181 GKVYEECGEALSDLSLKIVELLGLSLGVPKEKFKKFFEDNDSIIRLNYYPPCNKPELTLG 240
Query: 241 TGPHCDPTSITILHQDHVSGLQVYVDDQWYAIPPTPDSFVVNIGDTFMSLTNGVYKSCFH 300
TGPHCDPTSITILHQDHVSGLQVYVDDQW++IPP DSFV+NIGDTFM+LTNGVYKSCFH
Sbjct: 241 TGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYKSCFH 300
Query: 301 RAVVNPKETRKSVAFFLCPAADKLVRAPEELVEKNPPRKFPDYTWPMLLEMTQKFYRADS 360
RAVVN +E RKS+ FFLCPA DK+VRAP+E+V+KNPPRKFPDYTWPMLLE+TQKFYR+DS
Sbjct: 301 RAVVNCREARKSIVFFLCPADDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFYRSDS 360
Query: 361 NTFKAFTSWLQEQKKLEGGSCTVAPSV 386
NTFKAFT+WLQ K L + ++APS+
Sbjct: 361 NTFKAFTTWLQ-HKNLATAASSLAPSL 386
BLAST of Lag0019961 vs. ExPASy TrEMBL
Match:
A0A6J1E4L1 (gibberellin 20 oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111430571 PE=3 SV=1)
HSP 1 Score: 615.5 bits (1586), Expect = 1.5e-172
Identity = 289/387 (74.68%), Postives = 336/387 (86.82%), Query Frame = 0
Query: 1 MAIKCMTATRSSPSA--PGESKREHHLTFGSSGESKVPEDFIWPDEFKASEPVPELPVPH 60
MAIKCMT +S + E KR+H + FGSS ES VPE+FIWP+EFKA E VPEL VPH
Sbjct: 1 MAIKCMTTAKSQGESKLKQEMKRKHRVEFGSSDESMVPENFIWPEEFKARESVPELHVPH 60
Query: 61 IDMKKFLSGNESDVEEAARVVDKACRKHGFFVVVNHGVDMNMMKDVHECMDEFFSLPLEV 120
ID++KF+S NE+D+EE R+VD+ACRKHGFFV+VNHGVDM +MK+VHECMDEFFSLPL+V
Sbjct: 61 IDLQKFMSENENDIEEVRRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLPLDV 120
Query: 121 KEKAHRKVGENFGYANSFIGRFSNKLPWKETFSVRSLADENLPTAYNYVCEVLGQEFSHH 180
K+KA RKVGEN+GYANSFIGRFS+KLPWKETFS+R A P ++YVC LGQEFS
Sbjct: 121 KQKAQRKVGENYGYANSFIGRFSSKLPWKETFSLRYAAHH--PITHDYVCHHLGQEFSQQ 180
Query: 181 GKVYQEFGKAMSDLGLKIVELLGLSLGIQREHYKKFYEDNDSIIRLNYYPPCEKPELTLG 240
GKVY+E GKA+SDL LKIVELLGLSLG+ ++ +KKF+EDNDSIIRLNYYPPC KPELTLG
Sbjct: 181 GKVYEECGKALSDLSLKIVELLGLSLGVPKQKFKKFFEDNDSIIRLNYYPPCNKPELTLG 240
Query: 241 TGPHCDPTSITILHQDHVSGLQVYVDDQWYAIPPTPDSFVVNIGDTFMSLTNGVYKSCFH 300
TGPHCDPTSITILHQDHVSGLQVYVDDQW++IPP DSFV+NIGDTFM+LTNGVYKSCFH
Sbjct: 241 TGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYKSCFH 300
Query: 301 RAVVNPKETRKSVAFFLCPAADKLVRAPEELVEKNPPRKFPDYTWPMLLEMTQKFYRADS 360
RAVVN +E RKS+ FFLCPA DK+VRAP+E+V+KNPPRKFPDYTWPMLLE+TQKFYR+DS
Sbjct: 301 RAVVNCREARKSIVFFLCPAGDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFYRSDS 360
Query: 361 NTFKAFTSWLQEQKKLEGGSCTVAPSV 386
NTFKAFT+WLQ KKL + ++APS+
Sbjct: 361 NTFKAFTTWLQ-HKKLATAASSLAPSL 384
BLAST of Lag0019961 vs. ExPASy TrEMBL
Match:
A0A6J1G185 (gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449755 PE=3 SV=1)
HSP 1 Score: 604.4 bits (1557), Expect = 3.4e-169
Identity = 283/388 (72.94%), Postives = 331/388 (85.31%), Query Frame = 0
Query: 1 MAIKCMTATRSSPSA----PGESKREHHLTFGSSGESKVPEDFIWPDEFKASEPVPELPV 60
MA C AT+SS SA E KRE TFG S S VPE FIWPDEFK S+ +PEL V
Sbjct: 1 MAFNCKMATKSSLSAHLNLNQEMKRERRETFGGSDGSMVPEYFIWPDEFKGSQLLPELHV 60
Query: 61 PHIDMKKFLSGNESDVEEAARVVDKACRKHGFFVVVNHGVDMNMMKDVHECMDEFFSLPL 120
PHID+ KFLSG+ESDVEEA R+VD+ACR+HGFFVVVNHGVDM +MK VHEC+DEFF++PL
Sbjct: 61 PHIDLNKFLSGDESDVEEATRLVDEACREHGFFVVVNHGVDMELMKGVHECIDEFFTMPL 120
Query: 121 EVKEKAHRKVGENFGYANSFIGRFSNKLPWKETFSVRSLADENLPTAYNYVCEVLGQEFS 180
+VK++A RKVGEN+GY NSF GRF++KLPWKETFS+R +A +N TA +YV + LG FS
Sbjct: 121 DVKQRAQRKVGENYGYTNSFFGRFASKLPWKETFSLRCVAAQNSSTARDYVIDTLGSTFS 180
Query: 181 HHGKVYQEFGKAMSDLGLKIVELLGLSLGIQREHYKKFYEDNDSIIRLNYYPPCEKPELT 240
HHGKVYQE GKA++DLG+KI+E+LGLSLG+ RE++K FYEDNDSI+RLNYYPPCEKPE+
Sbjct: 181 HHGKVYQECGKALNDLGMKILEILGLSLGVSREYFKNFYEDNDSILRLNYYPPCEKPEVV 240
Query: 241 LGTGPHCDPTSITILHQDHVSGLQVYVDDQWYAIPPTPDSFVVNIGDTFMSLTNGVYKSC 300
LGTGPH DPTS+TILHQDHVSGLQV V+DQWY+IPPTPDSFVVNIGDTF SLTNG+YKSC
Sbjct: 241 LGTGPHTDPTSVTILHQDHVSGLQVCVNDQWYSIPPTPDSFVVNIGDTFTSLTNGIYKSC 300
Query: 301 FHRAVVNPKETRKSVAFFLCPAADKLVRAPEELVEKNPPRKFPDYTWPMLLEMTQKFYRA 360
HRAVVN KE RKS+AFF+CPA DK+VRAPEELVEKNPPRK+PDYTWPMLLEMTQK YRA
Sbjct: 301 VHRAVVNCKEARKSMAFFICPAHDKVVRAPEELVEKNPPRKYPDYTWPMLLEMTQKHYRA 360
Query: 361 DSNTFKAFTSWLQEQKKLEGGSCTVAPS 385
D NT +AFT+W+Q++K L+ G CT+ PS
Sbjct: 361 DCNTLEAFTTWIQKEKLLDTG-CTITPS 387
BLAST of Lag0019961 vs. TAIR 10
Match:
AT5G51810.1 (gibberellin 20 oxidase 2 )
HSP 1 Score: 429.9 bits (1104), Expect = 2.2e-120
Identity = 209/376 (55.59%), Postives = 271/376 (72.07%), Query Frame = 0
Query: 1 MAIKCMTAT----RSSPSAPGESKREHHLTFGSS---GESKVPEDFIWPDEFKASEPVPE 60
MAI C T + P E + L F S +S++P FIWPDE K S +PE
Sbjct: 1 MAILCTTTSPAEKEHEPKQDLEKDQTSPLIFNPSLLNLQSQIPNQFIWPDEEKPSIDIPE 60
Query: 61 LPVPHIDMKKFLSGNESDVEEAARVVDKACRKHGFFVVVNHGVDMNMMKDVHECMDEFFS 120
L VP ID LS +S + EA RV+ +AC KHGFF+VVNHGV +++ D H M+ FF
Sbjct: 61 LNVPFID----LSSQDSTL-EAPRVIAEACTKHGFFLVVNHGVSESLIADAHRLMESFFD 120
Query: 121 LPLEVKEKAHRKVGENFGYANSFIGRFSNKLPWKETFSVRSLADEN-LPTAYNYVCEVLG 180
+PL K+KA RK GE+ GYA+SF GRFS KLPWKET S + D + T +Y + LG
Sbjct: 121 MPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSNDNSGSRTVQDYFSDTLG 180
Query: 181 QEFSHHGKVYQEFGKAMSDLGLKIVELLGLSLGIQREHYKKFYEDNDSIIRLNYYPPCEK 240
QEF GKVYQ++ +AMS L LKI+ELLGLSLG+ R++++ F+E+NDSI+RLN+YPPC+
Sbjct: 181 QEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQT 240
Query: 241 PELTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWYAIPPTPDSFVVNIGDTFMSLTNGV 300
P+LTLGTGPHCDP+S+TILHQDHV+GLQV+VD+QW +I P P +FVVNIGDTFM+L+NG+
Sbjct: 241 PDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNGI 300
Query: 301 YKSCFHRAVVNPKETRKSVAFFLCPAADKLVRAPEELVEKNPPRKFPDYTWPMLLEMTQK 360
+KSC HRAVVN + RKS+AFFLCP DK+V+ P +++EK RK+PD+TW M LE TQK
Sbjct: 301 FKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQK 360
Query: 361 FYRADSNTFKAFTSWL 369
YRAD NT +F++W+
Sbjct: 361 HYRADVNTLDSFSNWV 371
BLAST of Lag0019961 vs. TAIR 10
Match:
AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )
HSP 1 Score: 421.4 bits (1082), Expect = 7.8e-118
Identity = 194/338 (57.40%), Postives = 253/338 (74.85%), Query Frame = 0
Query: 32 ESKVPEDFIWPDEFKASEPVPELPVPHIDMKKFLSGNESDVEEAARVVDKACRKHGFFVV 91
++ +P FIWPD+ K S V EL VP ID++ LS + S +A+R++ +AC+KHGFF+V
Sbjct: 37 QANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLV 96
Query: 92 VNHGVDMNMMKDVHECMDEFFSLPLEVKEKAHRKVGENFGYANSFIGRFSNKLPWKETFS 151
VNHG+ ++ D HE FF +PL K++ RK GE+ GYA+SF GRFS KLPWKET S
Sbjct: 97 VNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLS 156
Query: 152 VRSLAD-ENLPTAYNYVCEVLGQEFSHHGKVYQEFGKAMSDLGLKIVELLGLSLGIQREH 211
R D + +Y C+ LG F GKVYQE+ +AMS L LKI+ELLGLSLG++R++
Sbjct: 157 FRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDY 216
Query: 212 YKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWYAI 271
+++F+E+NDSI+RLNYYPPC KP+LTLGTGPHCDPTS+TILHQDHV+GLQV+V++QW +I
Sbjct: 217 FREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSI 276
Query: 272 PPTPDSFVVNIGDTFMSLTNGVYKSCFHRAVVNPKETRKSVAFFLCPAADKLVRAPEELV 331
P P +FVVNIGDTFM+L+N YKSC HRAVVN + RKS+AFFLCP D++V P EL+
Sbjct: 277 RPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELL 336
Query: 332 EKNPPRKFPDYTWPMLLEMTQKFYRADSNTFKAFTSWL 369
+ R++PD+TW M LE TQK YRAD NT +AF+ WL
Sbjct: 337 DSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373
BLAST of Lag0019961 vs. TAIR 10
Match:
AT5G07200.1 (gibberellin 20-oxidase 3 )
HSP 1 Score: 415.2 bits (1066), Expect = 5.6e-116
Identity = 193/342 (56.43%), Postives = 251/342 (73.39%), Query Frame = 0
Query: 35 VPEDFIWPDEFKASEPVPELPVPHIDMKKFLSGNESDVEEAARVVDKACRKHGFFVVVNH 94
+P+ F+WPD K S V L VP ID+ FLSG+ EA R+V KA KHGFF++ NH
Sbjct: 37 IPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNH 96
Query: 95 GVDMNMMKDVHECMDEFFSLPLEVKEKAHRKVGENFGYANSFIGRFSNKLPWKETFSVRS 154
GVD +++ + MD FF P K+KA RK GE+ GYA+SF+GRFS+KLPWKET S +
Sbjct: 97 GVDESLLSRAYLHMDSFFKAPACEKQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKF 156
Query: 155 LADENL--PTAYNYVCEVLGQEFSHHGKVYQEFGKAMSDLGLKIVELLGLSLGIQREHYK 214
+E + T ++V + +G + GKVYQE+ +AM+ L LKI+ELLG+SLG++R ++K
Sbjct: 157 SPEEKIHSQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFK 216
Query: 215 KFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWYAIPP 274
+F+ED+DSI RLNYYP C++PEL LGTGPHCDPTS+TILHQD V GLQV+VD++W +IPP
Sbjct: 217 EFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPP 276
Query: 275 TPDSFVVNIGDTFMSLTNGVYKSCFHRAVVNPKETRKSVAFFLCPAADKLVRAPEELVE- 334
P +FVVNIGDTFM+LTNG YKSC HRAVVN + RK+ AFFLCP +K+V+ PEELV
Sbjct: 277 NPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNG 336
Query: 335 -KNPPRKFPDYTWPMLLEMTQKFYRADSNTFKAFTSWLQEQK 373
K+ RK+PD+TW M LE TQK YRAD NT F+ WL+ ++
Sbjct: 337 VKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSIWLKNRR 378
BLAST of Lag0019961 vs. TAIR 10
Match:
AT1G60980.1 (gibberellin 20-oxidase 4 )
HSP 1 Score: 412.9 bits (1060), Expect = 2.8e-115
Identity = 192/341 (56.30%), Postives = 246/341 (72.14%), Query Frame = 0
Query: 35 VPEDFIWPDEFKASEPVPELPVPHIDMKKFLSGNESDVEEAARVVDKACRKHGFFVVVNH 94
+P++F+WPD K S+ VP L VP ID+ FLS + V EA R+V +A +KHGFF+V NH
Sbjct: 36 IPQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFLVTNH 95
Query: 95 GVDMNMMKDVHECMDEFFSLPLEVKEKAHRKVGENFGYANSFIGRFSNKLPWKET----F 154
GVD ++ H+ MD FF P K KA RKVGE GYA+SF+GRF LPWKET F
Sbjct: 96 GVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETLSFSF 155
Query: 155 SVRSLADENLPTAYNYVCEVLGQEFSHHGKVYQEFGKAMSDLGLKIVELLGLSLGIQREH 214
S ++ T NY+ + +G + G VYQE+ + MS+L LKI+ELLG+SLGI+REH
Sbjct: 156 SPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGIKREH 215
Query: 215 YKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWYAI 274
+++F+EDN+SI RLNYYP C++P+L LGTGPHCDPTS+TIL QD VSGLQV+VD+QW +I
Sbjct: 216 FREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQWQSI 275
Query: 275 PPTPDSFVVNIGDTFMSLTNGVYKSCFHRAVVNPKETRKSVAFFLCPAADKLVRAPEELV 334
PP P + VVNIGDT M+LTNG+YKSC HRAVVN + TRK++AFFLCP DK+V+ P EL
Sbjct: 276 PPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCPKVDKVVKPPSEL- 335
Query: 335 EKNPPRKFPDYTWPMLLEMTQKFYRADSNTFKAFTSWLQEQ 372
R +PD+TW M LE T K YRAD NT + FT+WL+ +
Sbjct: 336 --EGERAYPDFTWSMFLEFTMKHYRADMNTLEEFTNWLKNK 373
BLAST of Lag0019961 vs. TAIR 10
Match:
AT1G44090.1 (gibberellin 20-oxidase 5 )
HSP 1 Score: 302.8 bits (774), Expect = 4.1e-82
Identity = 151/341 (44.28%), Postives = 214/341 (62.76%), Query Frame = 0
Query: 32 ESKVPEDFIWPDEFKASEPVPELPVPHIDMKKFLSGNESDVEEAARVVDKACRKHGFFVV 91
+ VP +F WP++ A +L +P ID+ FL+GNE++ + AA+ V KAC HG F+V
Sbjct: 40 QPNVPAEFFWPEKDVAPSE-GDLDLPIIDLSGFLNGNEAETQLAAKAVKKACMAHGTFLV 99
Query: 92 VNHGVDMNMMKDVHECMDEFFSLPLEVKEKAHRKVGENFGYANSFIGRFSNKLPWKETFS 151
VNHG + + E FF L + K +A+R G GY RFS+ LPW ET
Sbjct: 100 VNHGFKSGLAEKALEISSLFFGLSKDEKLRAYRIPGNISGYTAGHSQRFSSNLPWNETL- 159
Query: 152 VRSLADENLP--TAYNYVCEVLGQEFSHHGKVYQEFGKAMSDLGLKIVELLGLSLGIQ-R 211
+LA + P +++ LG G+V+QEF AM+ L + ++ELLG+S+G++ R
Sbjct: 160 --TLAFKKGPPHVVEDFLTSRLGNHRQEIGQVFQEFCDAMNGLVMDLMELLGISMGLKDR 219
Query: 212 EHYKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWY 271
+Y++F+ED I R NYYPPC++PE LG GPH DPT+IT+L QD V GL+V+ W
Sbjct: 220 TYYRRFFEDGSGIFRCNYYPPCKQPEKALGVGPHNDPTAITVLLQDDVVGLEVFAAGSWQ 279
Query: 272 AIPPTPDSFVVNIGDTFMSLTNGVYKSCFHRAVVNPKETRKSVAFFLCPAADKLVRAPEE 331
+ P P + VVN+GDTFM+L+NG Y+SC+HRAVVN ++ R+S+ FF CP DK++ P E
Sbjct: 280 TVRPRPGALVVNVGDTFMALSNGNYRSCYHRAVVNKEKVRRSLVFFSCPREDKIIVPPPE 339
Query: 332 LVE-KNPPRKFPDYTWPMLLEMTQKFYRADSNTFKAFTSWL 369
LVE + RK+PD+TW L + TQ YR D+ T F+SWL
Sbjct: 340 LVEGEEASRKYPDFTWAQLQKFTQSGYRVDNTTLHNFSSWL 376
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
CAA70329.1 | 2.9e-175 | 79.51 | gibberellin 20-oxidase [Marah macrocarpa] | [more] |
XP_022984437.1 | 6.5e-175 | 75.45 | gibberellin 20 oxidase 2-like [Cucurbita maxima] | [more] |
CBL59326.1 | 1.9e-174 | 75.19 | Gibberellin 20-oxidase [Cucurbita maxima] | [more] |
XP_023552793.1 | 1.2e-173 | 74.68 | gibberellin 20 oxidase 2-like [Cucurbita pepo subsp. pepo] | [more] |
XP_022922616.1 | 3.0e-172 | 74.68 | gibberellin 20 oxidase 2-like [Cucurbita moschata] >KAG6576758.1 Gibberellin 20 ... | [more] |
Match Name | E-value | Identity | Description | |
Q39111 | 3.1e-119 | 55.59 | Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1 | [more] |
Q39110 | 1.1e-116 | 57.40 | Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2 | [more] |
Q39112 | 7.9e-115 | 56.43 | Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1 | [more] |
Q9C955 | 3.9e-114 | 56.30 | Gibberellin 20 oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=GA20OX4 PE=2 SV=1 | [more] |
O04707 | 1.1e-108 | 53.04 | Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
Q96498 | 1.4e-175 | 79.51 | Gibberellin 20-oxidase OS=Marah macrocarpa OX=48242 PE=2 SV=1 | [more] |
A0A6J1J263 | 3.2e-175 | 75.45 | gibberellin 20 oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482738 PE=3 S... | [more] |
I0J1I7 | 9.2e-175 | 75.19 | Gibberellin 20-oxidase OS=Cucurbita maxima OX=3661 GN=ga20ox4 PE=2 SV=1 | [more] |
A0A6J1E4L1 | 1.5e-172 | 74.68 | gibberellin 20 oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111430571 PE=3... | [more] |
A0A6J1G185 | 3.4e-169 | 72.94 | gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449755 PE=3... | [more] |