Lag0019900 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0019900
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr5: 46558676 .. 46559296 (+)
RNA-Seq ExpressionLag0019900
SyntenyLag0019900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATGGCGATAGGTTTCTGATTATCTATCCCATTTCAAGGCCTTTCAACGCAGGAAGGGTGGAGCTGTTGGGAGGGGGTGATTCTGAATCTAAGGTTTGGAACCCACCTGCGTTTCCTAATTACAAATTAAATACAGATGTGGCAGTGAACAAGAATCTCAATCGCAGCAGTATTGGTGCAGTTGTCAGAAACAAAAGAGGGGAAGTGATGCTTACCTCCGAGAGGTTGGTTGATTTTGTGATTGATGTGGAGGTATTGGAGGCGATGGAGGTTCGCGAAGGACTGGTTGCAGTGGTTGAAGCTGGCTTCTCGCAGGTGATGGCAGAATCAGATTCGGCAAGTGTGGTGAAGCAGATCAGTTCGACGCAGAGAAACCTCTCGGAGATGGGTTTTCTGGTTGAGGAAATTAGGAGCATTGCCAGCAGGTTGAGGTTTTGCTCGATCGGCTGGTGCTGTCGAGCTAGAAATAAGCTAGCGCACGAGGTAGCAAGGACAGCGTTAAGGCAGCAGCTGGAAGGAACTTGGCTGGAGGAGGTGTCGTTGGCTGTCGAAGGTTGCTACTTGGCTGAGTTGAGGAGCGCTGATGCGGATTCTAGGGCTTTATGTAATGAGTTTTAG

mRNA sequence

ATGAATGGCGATAGGTTTCTGATTATCTATCCCATTTCAAGGCCTTTCAACGCAGGAAGGGTGGAGCTGTTGGGAGGGGGTGATTCTGAATCTAAGGTTTGGAACCCACCTGCGTTTCCTAATTACAAATTAAATACAGATGTGGCAGTGAACAAGAATCTCAATCGCAGCAGTATTGGTGCAGTTGTCAGAAACAAAAGAGGGGAAGTGATGCTTACCTCCGAGAGGTTGGTTGATTTTGTGATTGATGTGGAGGTATTGGAGGCGATGGAGGTTCGCGAAGGACTGGTTGCAGTGGTTGAAGCTGGCTTCTCGCAGGTGATGGCAGAATCAGATTCGGCAAGTGTGGTGAAGCAGATCAGTTCGACGCAGAGAAACCTCTCGGAGATGGGTTTTCTGGTTGAGGAAATTAGGAGCATTGCCAGCAGGTTGAGGTTTTGCTCGATCGGCTGGTGCTGTCGAGCTAGAAATAAGCTAGCGCACGAGGTAGCAAGGACAGCGTTAAGGCAGCAGCTGGAAGGAACTTGGCTGGAGGAGGTGTCGTTGGCTGTCGAAGGTTGCTACTTGGCTGAGTTGAGGAGCGCTGATGCGGATTCTAGGGCTTTATGTAATGAGTTTTAG

Coding sequence (CDS)

ATGAATGGCGATAGGTTTCTGATTATCTATCCCATTTCAAGGCCTTTCAACGCAGGAAGGGTGGAGCTGTTGGGAGGGGGTGATTCTGAATCTAAGGTTTGGAACCCACCTGCGTTTCCTAATTACAAATTAAATACAGATGTGGCAGTGAACAAGAATCTCAATCGCAGCAGTATTGGTGCAGTTGTCAGAAACAAAAGAGGGGAAGTGATGCTTACCTCCGAGAGGTTGGTTGATTTTGTGATTGATGTGGAGGTATTGGAGGCGATGGAGGTTCGCGAAGGACTGGTTGCAGTGGTTGAAGCTGGCTTCTCGCAGGTGATGGCAGAATCAGATTCGGCAAGTGTGGTGAAGCAGATCAGTTCGACGCAGAGAAACCTCTCGGAGATGGGTTTTCTGGTTGAGGAAATTAGGAGCATTGCCAGCAGGTTGAGGTTTTGCTCGATCGGCTGGTGCTGTCGAGCTAGAAATAAGCTAGCGCACGAGGTAGCAAGGACAGCGTTAAGGCAGCAGCTGGAAGGAACTTGGCTGGAGGAGGTGTCGTTGGCTGTCGAAGGTTGCTACTTGGCTGAGTTGAGGAGCGCTGATGCGGATTCTAGGGCTTTATGTAATGAGTTTTAG

Protein sequence

MNGDRFLIIYPISRPFNAGRVELLGGGDSESKVWNPPAFPNYKLNTDVAVNKNLNRSSIGAVVRNKRGEVMLTSERLVDFVIDVEVLEAMEVREGLVAVVEAGFSQVMAESDSASVVKQISSTQRNLSEMGFLVEEIRSIASRLRFCSIGWCCRARNKLAHEVARTALRQQLEGTWLEEVSLAVEGCYLAELRSADADSRALCNEF
Homology
BLAST of Lag0019900 vs. NCBI nr
Match: XP_030963892.1 (uncharacterized protein LOC115985062 [Quercus lobata])

HSP 1 Score: 99.0 bits (245), Expect = 5.1e-17
Identity = 58/177 (32.77%), Postives = 96/177 (54.24%), Query Frame = 0

Query: 3   GDRFLIIYPISRPFNAGRVELLGGGDSESKVWNPPAFPNYKLNTDVAVNKNLNRSSIGAV 62
           G  FL+ Y  ++     ++ELL     +S  W PP   +YKLN D A+  +L  S +GA+
Sbjct: 9   GSYFLLEYHQAQ----DQLELLQNAQRQSSGWQPPHSDHYKLNFDAAIYTDLGCSGVGAI 68

Query: 63  VRNKRGEVMLTSERLVDFVIDVEVLEAMEVREGLVAVVEAGFSQVMAESDSASVVKQISS 122
           +RN+RGEVM        FV D E  E +  R+ L   ++ G S ++ E D+ SV++ ++S
Sbjct: 69  IRNERGEVMAALSAKGPFVQDSEEAETLACRKALEFAIDVGISDLIIEGDNVSVMRAVAS 128

Query: 123 TQRNLSEMGFLVEEIRSIASRLRFCSIGWCCRARNKLAHEVARTALRQQLEGTWLEE 180
              + S +G ++E+I  +   LR+ S+    R+ N +AH ++R A     E  W+E+
Sbjct: 129 NCMDGSRLGNILEDIHWLVRSLRWSSVSCVKRSANTVAHSLSRFARNVPDELVWIED 181

BLAST of Lag0019900 vs. NCBI nr
Match: GAV89685.1 (RVT_3 domain-containing protein, partial [Cephalotus follicularis])

HSP 1 Score: 97.8 bits (242), Expect = 1.1e-16
Identity = 54/149 (36.24%), Postives = 84/149 (56.38%), Query Frame = 0

Query: 34  WNPPAFPNYKLNTDVAVNKNLNRSSIGAVVRNKRGEVMLTSERLVDFVIDVEVLEAMEVR 93
           W PP     K+N D AV++N N    G VVR+ +G V+L +   V+ + D   +EAM + 
Sbjct: 2   WCPPPRQTLKVNCDAAVSENRNDVGAGMVVRDVQGNVLLAAAMTVEHIRDPRSIEAMAIL 61

Query: 94  EGLVAVVEAGFSQVMAESDSASVVKQISSTQRNLSEMGFLVEEIRSIASRLRFCSIGWCC 153
             L   +E G+ +++ ESD+ +V+ +I++ Q +LS  G +VEEI+S+ S   FC   W  
Sbjct: 62  HALKVSLEKGWRKIIVESDAENVLNEINAYQPSLSLYGNVVEEIKSLVSPFIFCQFSWIS 121

Query: 154 RARNKLAHEVARTALRQQLEGTWLEEVSL 183
           +  NK+AHE+AR A        W+E   L
Sbjct: 122 KVGNKMAHELARLASSVYTNMVWIESFCL 150

BLAST of Lag0019900 vs. NCBI nr
Match: XP_023876296.1 (uncharacterized protein LOC111988743 [Quercus suber])

HSP 1 Score: 96.3 bits (238), Expect = 3.3e-16
Identity = 55/149 (36.91%), Postives = 86/149 (57.72%), Query Frame = 0

Query: 31  SKVWNPPAFPNYKLNTDVAVNKNLNRSSIGAVVRNKRGEVMLTSERLVDFVIDVEVLEAM 90
           + +W  P  P YKLN D A+ K LN S IGA++RN +GEVM         V D E  E +
Sbjct: 231 ANIWVAPTDPVYKLNFDAAIFKELNCSGIGAIIRNGKGEVMAAMSAKGPLVEDSEEAETL 290

Query: 91  EVREGLVAVVEAGFSQVMAESDSASVVKQISSTQRNLSEMGFLVEEIRSIASRLRFCSIG 150
             R+ +   ++AGFS+++ E D+A+V+  +S    +LS +G +V++++ +A+ LR+ S  
Sbjct: 291 ACRKAVEFAIDAGFSELVIEGDNAAVMASLSHGGPDLSRLGHVVQDVQWLATGLRWVSFS 350

Query: 151 WCCRARNKLAHEVARTALRQQLEGTWLEE 180
              R  N +AH +AR A     +  WLEE
Sbjct: 351 HVKRCANTVAHGLARHAKNVMEDMIWLEE 379

BLAST of Lag0019900 vs. NCBI nr
Match: XP_023904177.1 (uncharacterized protein LOC112015942 [Quercus suber])

HSP 1 Score: 96.3 bits (238), Expect = 3.3e-16
Identity = 55/158 (34.81%), Postives = 93/158 (58.86%), Query Frame = 0

Query: 33  VWNPPAFPNYKLNTDVAVNKNLNRSSIGAVVRNKRGEVMLTSERLVDFVIDVEVLEAMEV 92
           VW PPA P +K+N D A+  +  RS  GAV+RN+ GEVM     +   V   +  E +  
Sbjct: 349 VWRPPAHPFFKMNFDAAIFNDSGRSGFGAVIRNELGEVMAALSAIGPSVSSSDEAETLAC 408

Query: 93  REGLVAVVEAGFSQVMAESDSASVVKQISSTQRNLSEMGFLVEEIRSIASRLRFCSIGWC 152
           R+ +   V+AGF++++ E DS +V+K +SS+  +LS +G +V +I+ + S LR  S  W 
Sbjct: 409 RKAMEFAVDAGFNELIIEGDSINVIKALSSSSPDLSVVGNVVADIKCLISGLRRVSFSWV 468

Query: 153 CRARNKLAHEVARTALRQQLEGTWLEEV-SLAVEGCYL 190
            R  N++AH +A+ A   + +  W+E+   +A++  +L
Sbjct: 469 NRDCNRVAHALAKFANSLEEDLYWMEDAPPVALDALFL 506

BLAST of Lag0019900 vs. NCBI nr
Match: XP_030963999.1 (uncharacterized protein LOC115985177 [Quercus lobata])

HSP 1 Score: 95.1 bits (235), Expect = 7.4e-16
Identity = 57/148 (38.51%), Postives = 84/148 (56.76%), Query Frame = 0

Query: 32  KVWNPPAFPNYKLNTDVAVNKNLNRSSIGAVVRNKRGEVMLTSERLVDFVIDVEVLEAME 91
           ++W PP    YK+N DV V   +N S IG VVRNKRGEVM++       + D E  E + 
Sbjct: 86  QLWEPPVGLIYKVNVDVVVFAEINASGIGVVVRNKRGEVMVSLVAKGPLMQDSEEAEVLA 145

Query: 92  VREGLVAVVEAGFSQVMAESDSASVVKQISSTQRNLSEMGFLVEEIRSIASRLRFCSIGW 151
            ++ L  VV+A F+ ++ E D+ SV+K I+S + N S +G + E+I  IA   R  S+ +
Sbjct: 146 CQKVLEFVVDASFTDLLLEGDNMSVMKSITSIRSNRSRLGHIYEDIHCIAVGFRSFSVNF 205

Query: 152 CCRARNKLAHEVARTALRQQLEGTWLEE 180
              + N +AH +A+ A     E  WLEE
Sbjct: 206 VKHSANLVAHSIAKFASNVDDELVWLEE 233

BLAST of Lag0019900 vs. ExPASy TrEMBL
Match: A0A1Q3DB78 (RVT_3 domain-containing protein (Fragment) OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_33099 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 5.5e-17
Identity = 54/149 (36.24%), Postives = 84/149 (56.38%), Query Frame = 0

Query: 34  WNPPAFPNYKLNTDVAVNKNLNRSSIGAVVRNKRGEVMLTSERLVDFVIDVEVLEAMEVR 93
           W PP     K+N D AV++N N    G VVR+ +G V+L +   V+ + D   +EAM + 
Sbjct: 2   WCPPPRQTLKVNCDAAVSENRNDVGAGMVVRDVQGNVLLAAAMTVEHIRDPRSIEAMAIL 61

Query: 94  EGLVAVVEAGFSQVMAESDSASVVKQISSTQRNLSEMGFLVEEIRSIASRLRFCSIGWCC 153
             L   +E G+ +++ ESD+ +V+ +I++ Q +LS  G +VEEI+S+ S   FC   W  
Sbjct: 62  HALKVSLEKGWRKIIVESDAENVLNEINAYQPSLSLYGNVVEEIKSLVSPFIFCQFSWIS 121

Query: 154 RARNKLAHEVARTALRQQLEGTWLEEVSL 183
           +  NK+AHE+AR A        W+E   L
Sbjct: 122 KVGNKMAHELARLASSVYTNMVWIESFCL 150

BLAST of Lag0019900 vs. ExPASy TrEMBL
Match: A0A2N9HRK6 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS42141 PE=3 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 1.5e-14
Identity = 53/163 (32.52%), Postives = 86/163 (52.76%), Query Frame = 0

Query: 29   SESKVWNPPAFPNYKLNTDVAVNKNLNRSSIGAVVRNKRGEVMLTSERLVDFVIDVEVLE 88
            +++  W PP+  N+K N D A+ K  N + IG +VRN+ G+VM +  + V +   VE +E
Sbjct: 1706 AQTTKWQPPSCQNFKANYDGAIFKETNEAGIGVIVRNREGKVMASLVQKVRYPQSVESIE 1765

Query: 89   AMEVREGLVAVVEAGFSQVMAESDSASVVKQISSTQRNLSEMGFLVEEIRSIASRLRFCS 148
            A   R  +  V E G S+   E DS +VV  ++S   NL+  G L+E+++ + + L    
Sbjct: 1766 AWAARRAVSFVAEIGLSEAEFEGDSKTVVAALNSPYPNLALYGHLIEDVKLLTNGLVSSC 1825

Query: 149  IGWCCRARNKLAHEVARTALRQQLEGTWLEEVSLAVEGCYLAE 192
                 R  N +AH +AR A+       W+E+V    +  YL+E
Sbjct: 1826 FTHVKRQGNAMAHALARMAINTANTEVWMEDVPPQTKRLYLSE 1868

BLAST of Lag0019900 vs. ExPASy TrEMBL
Match: A0A7N2LST3 (RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 1.5e-14
Identity = 56/153 (36.60%), Postives = 84/153 (54.90%), Query Frame = 0

Query: 27  GDSESKVWNPPAFPNYKLNTDVAVNKNLNRSSIGAVVRNKRGEVMLTSERLVDFVIDVEV 86
           G  + + W PP   +YK+N D AV   +N S  G VVRN+R EVM +       V D E 
Sbjct: 2   GVEQVQQWEPPTGYSYKINVDAAVFAEINASGFGVVVRNERAEVMASLAAKGPPVQDSEE 61

Query: 87  LEAMEVREGLVAVVEAGFSQVMAESDSASVVKQISSTQRNLSEMGFLVEEIRSIASRLRF 146
            + +  R+ L   V+AGF  V+ E DS +V++ IS+ + N S +G + E+I+ +A+  R 
Sbjct: 62  AKLLACRKALEFAVDAGFMDVVLEGDSLNVMRAISTGKANRSRLGHIYEDIQCLAAGFRS 121

Query: 147 CSIGWCCRARNKLAHEVARTALRQQLEGTWLEE 180
            S+ +  R+ N +AH +A  A     E  WLEE
Sbjct: 122 YSVSFVKRSANGVAHALACFAKNVGDELVWLEE 154

BLAST of Lag0019900 vs. ExPASy TrEMBL
Match: A0A7N2RFA1 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 7.5e-14
Identity = 55/162 (33.95%), Postives = 85/162 (52.47%), Query Frame = 0

Query: 34  WNPPAFPNYKLNTDVAVNKNLNRSSIGAVVRNKRGEVMLTSERLVDFVIDVEVLEAMEVR 93
           W PP    +KLN D A+  +L  S IGA++RN+RGEVM         V+D    E +  R
Sbjct: 199 WCPPPNSRFKLNFDAAIFPDLKCSGIGAIIRNERGEVMGAMSAKEQQVLDSTEAEVLACR 258

Query: 94  EGLVAVVEAGFSQVMAESDSASVVKQISSTQRNLSEMGFLVEEIRSIASRLRFCSIGWCC 153
             L   ++ GFS+++ E DSA V+  + ST  NLS +G + ++I+ + + L +  +    
Sbjct: 259 RALEFAIDIGFSELVIEGDSAQVLNSLRSTATNLSRLGHIFDDIQCLMAGLLWVEVKLVS 318

Query: 154 RARNKLAHEVARTALRQQLEGTWLEE-VSLAVEGCYLAELRS 195
           R  N   H +AR A     +  WLE+    A+E  Y+  L+S
Sbjct: 319 RNANGAPHSLARYAKNIVEDVVWLEDSPPPALEALYVDSLQS 360

BLAST of Lag0019900 vs. ExPASy TrEMBL
Match: A0A1Q3CFW0 (RVT_3 domain-containing protein (Fragment) OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_22463 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.3e-13
Identity = 49/137 (35.77%), Postives = 81/137 (59.12%), Query Frame = 0

Query: 31  SKVWNPPAFPNYKLNTDVAVNKNLNRSSIGAVVRNKRGEVMLTSERLVDFVIDVEVLEAM 90
           S  W  P+    K+N D AV++N N      VVR+ +G V+L + R V+ + D   +EA+
Sbjct: 1   STKWCLPSRQTLKVNCDGAVSENNNDRGACMVVRDMQGTVLLVATRTVEHIRDPPSIEAV 60

Query: 91  EVREGLVAVVEAGFSQVMAESDSASVVKQISSTQRNLSEMGFLVEEIRSIASRLRFCSIG 150
            +   L   +E G+ +++ ESD  +V+ +I+++Q +LS  G ++EEI+SI S   FC   
Sbjct: 61  AILHALKVSLEKGWRKIIIESDDENVLNEINASQPSLSLYGNVIEEIKSIVSSFIFCQFS 120

Query: 151 WCCRARNKLAHEVARTA 168
           W  R  NK+AH++A+ A
Sbjct: 121 WISRVGNKVAHKLAKLA 137

BLAST of Lag0019900 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 45.8 bits (107), Expect = 4.8e-05
Identity = 37/135 (27.41%), Postives = 65/135 (48.15%), Query Frame = 0

Query: 34  WNPPAFPNYKLNTDVAVNKNLNRSSIGAVVRNKRGEVMLTSERLVDFVIDVEVLEAMEVR 93
           W PP     K NTD   N++  R  IG V+RN++GEV     R +  +  V   E   +R
Sbjct: 420 WRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMR 479

Query: 94  EGLVAVVEAGFSQVMAESDSASVVKQISSTQRNLSEMGFLVEEIRSIASRLRFCSIGWCC 153
             ++++    ++ V+ ESDS  V+ +I +       +   +++++ + S+       +  
Sbjct: 480 WAVLSLSRFQYNYVIFESDS-QVLIEILNNDEIWPSLKPTIQDLQRLLSQFTEVKFVFIP 539

Query: 154 RARNKLAHEVARTAL 169
           R  N LA  VAR +L
Sbjct: 540 REGNTLAERVARESL 553

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_030963892.15.1e-1732.77uncharacterized protein LOC115985062 [Quercus lobata][more]
GAV89685.11.1e-1636.24RVT_3 domain-containing protein, partial [Cephalotus follicularis][more]
XP_023876296.13.3e-1636.91uncharacterized protein LOC111988743 [Quercus suber][more]
XP_023904177.13.3e-1634.81uncharacterized protein LOC112015942 [Quercus suber][more]
XP_030963999.17.4e-1638.51uncharacterized protein LOC115985177 [Quercus lobata][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A1Q3DB785.5e-1736.24RVT_3 domain-containing protein (Fragment) OS=Cephalotus follicularis OX=3775 GN... [more]
A0A2N9HRK61.5e-1432.52Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS42141 PE=3 SV=1[more]
A0A7N2LST31.5e-1436.60RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
A0A7N2RFA17.5e-1433.95Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
A0A1Q3CFW01.3e-1335.77RVT_3 domain-containing protein (Fragment) OS=Cephalotus follicularis OX=3775 GN... [more]
Match NameE-valueIdentityDescription
AT4G29090.14.8e-0527.41Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 41..174
e-value: 7.9E-14
score: 53.7
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 45..166
e-value: 7.7E-25
score: 87.1
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 31..189
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 31..189
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 44..165
e-value: 5.93215E-17
score: 71.5764
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 42..172

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0019900.1Lag0019900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity