Lag0019844 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0019844 vs. NCBI nr
Match: XP_038902857.1 (MATH domain and coiled-coil domain-containing protein At3g58400-like [Benincasa hispida])

HSP 1 Score: 447.6 bits (1150), Expect = 1.0e-121
Identity = 220/297 (74.07%), Postives = 254/297 (85.52%), Query Frame = 0

Query: 60  ITRSRRIDPPADYTFRIKSYSVLSEIGMMKCESGDFEIDGYKWKLILYPNGDEIGDDHIS 119
           ITRSRR+DPP+ Y F+I SYSV SEI M KCESGDFE+DGYKWKLILYPNGDE   DHIS
Sbjct: 20  ITRSRRVDPPSHYAFKIDSYSVFSEIEMKKCESGDFEVDGYKWKLILYPNGDEEVQDHIS 79

Query: 120 LYLAISSNKDHNLPLGWEVKVIFRFFIFDQIKDSYLTIQDGKVRKYSKMKSEHGFSHLLS 179
           LYLAIS+ KD NLPLGWEV+VIFRFFIFDQI+D+YLTIQDGK+R+YSKMKSEHGF+ LLS
Sbjct: 80  LYLAISA-KD-NLPLGWEVRVIFRFFIFDQIRDNYLTIQDGKMRRYSKMKSEHGFTQLLS 139

Query: 180 HIDFNKASNGFLVDNCCTFGVEVSLLKPSNKGEILTIIKEPQQLESSYWSFTSFSTKTDE 239
           H  FNKASNGFLVDN CTFGVE+S+LK SNKGEILTI KEPQQ ++ +WS  SFS K +E
Sbjct: 140 HNIFNKASNGFLVDNSCTFGVEISVLKASNKGEILTIYKEPQQ-DTFFWSIYSFSVKINE 199

Query: 240 SYVSYPINVKGRKWRIKIYPNGFSSGKTTHLSLYLKLDSSNTIPVGKKIYAKYILSVYNR 299
            Y+S P NVKGR+WR+ IYPNG S GKT+H+SLYL+LDSS TIP+GKKIYAK++L VYN 
Sbjct: 200 FYISEPFNVKGRQWRMVIYPNGHSRGKTSHISLYLRLDSSETIPLGKKIYAKFLLGVYNF 259

Query: 300 VDRKYIDKPARQRWFDNTTYGGGFHEFLARKDLSNYLDGDVLYLKARIVAMSTVEEF 357
           V +KYIDK +++ WF  T+ G GFHEFL+RKD+S +L  DVLYLKARIVAMS VEEF
Sbjct: 260 VAKKYIDK-SKEVWFAATSTGNGFHEFLSRKDMSPHLQNDVLYLKARIVAMSAVEEF 312

BLAST of Lag0019844 vs. NCBI nr
Match: XP_023529004.1 (speckle-type POZ protein-like A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 438.3 bits (1126), Expect = 6.1e-119
Identity = 214/299 (71.57%), Postives = 252/299 (84.28%), Query Frame = 0

Query: 59  QITRSRRIDPPADYTFRIKSYSVLSEIGMMKCESGDFEIDGYKWKLILYPNGDEIGDDHI 118
           +ITR+RR++PPA+YTF+I SYS LSEIGM KCESG+F +DGY WKLILYPNGD+  +DHI
Sbjct: 18  EITRTRRVEPPANYTFQINSYSTLSEIGMKKCESGNFAVDGYNWKLILYPNGDDEVEDHI 77

Query: 119 SLYLAISSNKDHNLPLGWEVKVIFRFFIFDQIKDSYLTIQDGKVRKYSKMKSEHGFSHLL 178
           SLYLAI S KD NLPLGWEVKVIFRFF++D+IKD+YLT+QDGK+RKYSKMKSEHGF+  L
Sbjct: 78  SLYLAIIS-KD-NLPLGWEVKVIFRFFVYDRIKDNYLTVQDGKMRKYSKMKSEHGFTKFL 137

Query: 179 SHIDFNKASNGFLVDNCCTFGVEVSLLKPSNKGEILTIIKEPQQLESSYWSFTSFSTKTD 238
           +H  FN+ASNGFLVDN CTFGVEVS+LK SNKGE LTI KEP QL S +WS +SFS KT+
Sbjct: 138 AHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEP-QLGSFFWSISSFSNKTN 197

Query: 239 ESYVSYPINVKGRKWRIKIYPNGFSSGKTTHLSLYLKLDSSNTIPVGKKIYAKYILSVYN 298
           E Y+S P  VKGRKWR+ IYPNG SSGKT+H+SLYLKLDSS T+P GKKIYAK+ LSVYN
Sbjct: 198 EFYLSEPFTVKGRKWRLMIYPNGLSSGKTSHISLYLKLDSSETVPTGKKIYAKFFLSVYN 257

Query: 299 RVDRKYIDKPARQRWFDNTTYGGGFHEFLARKDLS-NYLDGDVLYLKARIVAMSTVEEF 357
              +KY+D+ + + WFDNT YG GFHEFL+R DL  ++L  DV YLKARIVAMS VE+F
Sbjct: 258 FGAKKYVDQ-SNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYLKARIVAMSVVEDF 312

BLAST of Lag0019844 vs. NCBI nr
Match: KAG6600198.1 (Ubiquitin carboxyl-terminal hydrolase 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 436.4 bits (1121), Expect = 2.3e-118
Identity = 212/299 (70.90%), Postives = 252/299 (84.28%), Query Frame = 0

Query: 59  QITRSRRIDPPADYTFRIKSYSVLSEIGMMKCESGDFEIDGYKWKLILYPNGDEIGDDHI 118
           +ITR+RR++PPA+YTF+I SYS LSEIGM KCESG+F +DG+ WKLILYPNGD+  +DHI
Sbjct: 105 EITRTRRVEPPANYTFQINSYSTLSEIGMKKCESGNFAVDGFNWKLILYPNGDDEVEDHI 164

Query: 119 SLYLAISSNKDHNLPLGWEVKVIFRFFIFDQIKDSYLTIQDGKVRKYSKMKSEHGFSHLL 178
           SLYLAI+S KD NLPLGWEVKVIFRFF++D+IKD+YLT+QDGK+RKYSKMKSEHGF+  L
Sbjct: 165 SLYLAITS-KD-NLPLGWEVKVIFRFFVYDRIKDNYLTVQDGKMRKYSKMKSEHGFTKFL 224

Query: 179 SHIDFNKASNGFLVDNCCTFGVEVSLLKPSNKGEILTIIKEPQQLESSYWSFTSFSTKTD 238
           +H  FN+ASNGFLVDN CTFGVEVS+LK SNKGE LTI KEP QL S +WS +SFS KT+
Sbjct: 225 AHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEP-QLGSFFWSISSFSNKTN 284

Query: 239 ESYVSYPINVKGRKWRIKIYPNGFSSGKTTHLSLYLKLDSSNTIPVGKKIYAKYILSVYN 298
           E Y+S P  VKGRKWR+ IYPNG SSGK +H+SLYLKLDSS T+P GKKIYAK+ LSVYN
Sbjct: 285 EFYLSEPFTVKGRKWRLTIYPNGLSSGKMSHISLYLKLDSSETVPTGKKIYAKFFLSVYN 344

Query: 299 RVDRKYIDKPARQRWFDNTTYGGGFHEFLARKDLS-NYLDGDVLYLKARIVAMSTVEEF 357
              +KY+D+ + + WFDNTTYG GFHEFL+R DL  ++L  DV YLKARI AMS VE+F
Sbjct: 345 FGAKKYVDQ-SNEHWFDNTTYGYGFHEFLSRNDLQPHHLQNDVFYLKARIAAMSVVEDF 399

BLAST of Lag0019844 vs. NCBI nr
Match: XP_022989347.1 (uncharacterized protein LOC111486429 [Cucurbita maxima])

HSP 1 Score: 431.4 bits (1108), Expect = 7.5e-117
Identity = 212/299 (70.90%), Postives = 248/299 (82.94%), Query Frame = 0

Query: 59  QITRSRRIDPPADYTFRIKSYSVLSEIGMMKCESGDFEIDGYKWKLILYPNGDEIGDDHI 118
           +ITR+ R+DPPA+Y F+I SYS LSEIGM KCESG F +DGY WKLIL+PNGD+  +DHI
Sbjct: 18  EITRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYNWKLILFPNGDDKVEDHI 77

Query: 119 SLYLAISSNKDHNLPLGWEVKVIFRFFIFDQIKDSYLTIQDGKVRKYSKMKSEHGFSHLL 178
           SLYLAI+S KD NLPLGWEVKVIFRFF++DQIKD+YLTIQDGK+RKYSKMK+EHGF+  L
Sbjct: 78  SLYLAITS-KD-NLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMRKYSKMKTEHGFTKFL 137

Query: 179 SHIDFNKASNGFLVDNCCTFGVEVSLLKPSNKGEILTIIKEPQQLESSYWSFTSFSTKTD 238
           +H  FN+ASNGFLVDN CTFGVEVS+LK SNKGE LTI KEP QL S +WS +SFS K +
Sbjct: 138 AHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEP-QLGSFFWSISSFSNKNN 197

Query: 239 ESYVSYPINVKGRKWRIKIYPNGFSSGKTTHLSLYLKLDSSNTIPVGKKIYAKYILSVYN 298
           E Y S P  VKGRKWR+ IYPNGF SGKT+H+SLYLKLDSS TIP GKKIYAK+ LSVYN
Sbjct: 198 EFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPTGKKIYAKFFLSVYN 257

Query: 299 RVDRKYIDKPARQRWFDNTTYGGGFHEFLARKDLS-NYLDGDVLYLKARIVAMSTVEEF 357
              +KY+D+ + + WFDNT YG GFHEFL+R DL  ++L  DV YLKARIVAMS V++F
Sbjct: 258 FGAKKYVDQ-SNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYLKARIVAMSVVQDF 312

BLAST of Lag0019844 vs. NCBI nr
Match: XP_008453868.1 (PREDICTED: uncharacterized protein LOC103494460 isoform X1 [Cucumis melo] >KAA0044674.1 ubiquitin carboxyl-terminal hydrolase 12-like isoform X2 [Cucumis melo var. makuwa] >TYK16908.1 ubiquitin carboxyl-terminal hydrolase 12-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 430.6 bits (1106), Expect = 1.3e-116
Identity = 204/295 (69.15%), Postives = 250/295 (84.75%), Query Frame = 0

Query: 62  RSRRIDPPADYTFRIKSYSVLSEIGMMKCESGDFEIDGYKWKLILYPNGDEIGDDHISLY 121
           +SRR+DPP+ Y F+I SYSVLSEI M KCESGDFE+DGY WKLILYPNG+   +DHISL+
Sbjct: 22  KSRRVDPPSHYAFQIDSYSVLSEIEMKKCESGDFEVDGYMWKLILYPNGNGEVEDHISLF 81

Query: 122 LAISSNKDHNLPLGWEVKVIFRFFIFDQIKDSYLTIQDGKVRKYSKMKSEHGFSHLLSHI 181
           LAIS+N ++NLPLGWEV+VIFRFFIFDQI+D+YLTIQDGK+RKYSKMKSEHGF+HL+SH 
Sbjct: 82  LAISTNDNNNLPLGWEVRVIFRFFIFDQIRDNYLTIQDGKMRKYSKMKSEHGFTHLISHN 141

Query: 182 DFNKASNGFLVDNCCTFGVEVSLLKPSNKGEILTIIKEPQQLESSYWSFTSFSTKTDESY 241
            F+KASNGFLV+N CTFGVEVS+LK SNKGE LTI +EPQQ ++ +W+  SFS KT++SY
Sbjct: 142 VFSKASNGFLVNNSCTFGVEVSILKASNKGERLTIFREPQQ-DTYFWTLYSFSAKTNQSY 201

Query: 242 VSYPINVKGRKWRIKIYPNGFSSGKTTHLSLYLKLDSSNTIPVGKKIYAKYILSVYNRVD 301
           +S P NVKGRKWR++IYPNG S GKT+H+SLYLKLD+S TIP+GKKIYAK++L VYN   
Sbjct: 202 ISEPFNVKGRKWRMEIYPNGNSLGKTSHISLYLKLDNSETIPLGKKIYAKFLLGVYNFSA 261

Query: 302 RKYIDKPARQRWFDNTTYGGGFHEFLARKDLSNYLDGDVLYLKARIVAMSTVEEF 357
           +KYIDK + + WF    +G GF EFL+R+++S +   D  YLKARIVAMSTV+EF
Sbjct: 262 KKYIDK-SYEHWFKTPGHGDGFDEFLSRREISTHSQNDAFYLKARIVAMSTVDEF 314

BLAST of Lag0019844 vs. ExPASy Swiss-Prot
Match: Q9M2J1 (MATH domain and coiled-coil domain-containing protein At3g58250 OS=Arabidopsis thaliana OX=3702 GN=At3g58250 PE=4 SV=2)

HSP 1 Score: 71.6 bits (174), Expect = 2.0e-11
Identity = 40/135 (29.63%), Postives = 73/135 (54.07%), Query Frame = 0

Query: 72  YTFRIKSYSVLSEIGMMKCESGDFEIDGYKWKLILYPNGDEIGDDHISLYLAISSNKDHN 131
           +++ IK++S L      K  S  F IDG +W+L+ +P G++   DH+SLYL ++ ++  +
Sbjct: 11  FSWVIKNFSSLQS---EKIYSDQFVIDGCRWRLLAFPKGNDTKSDHLSLYLDVAESE--S 70

Query: 132 LPLGWEVKVIFRFFIFDQIKDSYLTIQDGKVRKYSKMKSEHGFSHLLSHIDFNKASNGFL 191
           LP GW     F F I + I +   + +   +  + +  S+ GF++L+  I+     +GFL
Sbjct: 71  LPCGWRRHAQFSFTIVNHIPEK-CSQRKETIHWFCEKVSDWGFTNLVPLIELKAEDSGFL 130

Query: 192 VDNCCTFGVEVSLLK 207
           V       VE+ +L+
Sbjct: 131 VKGELKIVVEIEVLE 139

BLAST of Lag0019844 vs. ExPASy Swiss-Prot
Match: Q9M2I9 (MATH domain and coiled-coil domain-containing protein At3g58270 OS=Arabidopsis thaliana OX=3702 GN=At3g58270 PE=2 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.7e-10
Identity = 43/142 (30.28%), Postives = 75/142 (52.82%), Query Frame = 0

Query: 72  YTFRIKSYSVLSEIGMMKCESGDFEIDGYKWKLILYPNGDEIGDDHISLYLAISSNKDHN 131
           +T+ IK++S        K  S +F +DG KW+L+ +P G+  G + +SLYLA++ ++   
Sbjct: 9   FTWVIKNFSSQQS---RKNYSDEFFVDGCKWRLLAFPKGN--GVEKLSLYLAVAGSE--F 68

Query: 132 LPLGWEVKVIFRFFIFDQIKDSYLTIQDGKVRKYSKMKSEHGFSHLLSHIDFNKASNGFL 191
           LP GW     F F + +Q+ D     ++ K   +    S+ GF+ +LS    +    GFL
Sbjct: 69  LPDGWRRHAYFHFSVVNQLSDELSQARETK-NWFDASTSDWGFTSMLSLKKLHDKDGGFL 128

Query: 192 VDNCCTFGVEVSLLKPSNKGEI 214
           V+      V+VS+L+   K ++
Sbjct: 129 VNGELKIVVDVSVLEVIGKLDV 142

BLAST of Lag0019844 vs. ExPASy Swiss-Prot
Match: Q84WU2 (Ubiquitin C-terminal hydrolase 13 OS=Arabidopsis thaliana OX=3702 GN=UBP13 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.9e-10
Identity = 37/141 (26.24%), Postives = 76/141 (53.90%), Query Frame = 0

Query: 67  DPPA-DYTFRIKSYSVLSEIGMMKCESGDFEIDGYKWKLILYPNGDEIGDDHISLYLAIS 126
           DPP+  +T+ I  +   + +   K  S  F + GYKW+++++P G+ +  DH+S+YL ++
Sbjct: 50  DPPSLKFTWTIPMF---TRLNTRKHYSDVFVVGGYKWRILIFPKGNNV--DHLSMYLDVA 109

Query: 127 SNKDHNLPLGWEVKVIFRFFIFDQIKDSYLTIQDGKVRKYSKMKSEHGFSHLLSHIDFNK 186
                NLP GW     F   + +Q+ + Y +I+     +++  +S+ GF+  +   +  +
Sbjct: 110 DAA--NLPYGWSRYSQFSLAVVNQVNNRY-SIRKETQHQFNARESDWGFTSFMPLSELYE 169

Query: 187 ASNGFLVDNCCTFGVEVSLLK 207
            + G+LV++      EV++ K
Sbjct: 170 PTRGYLVNDTVLIEAEVAVRK 182

BLAST of Lag0019844 vs. ExPASy Swiss-Prot
Match: Q9FPT1 (Ubiquitin C-terminal hydrolase 12 OS=Arabidopsis thaliana OX=3702 GN=UBP12 PE=1 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 3.2e-09
Identity = 36/131 (27.48%), Postives = 62/131 (47.33%), Query Frame = 0

Query: 228 WSFTSFSTKTDESYVSYPINVKGRKWRIKIYPNGFSSGKTTHLSLYLKLDSSNTIPVGKK 287
           W+  +FS +    + S    V G KWRI I+P G       HLS+YL +  + ++P G  
Sbjct: 59  WTIPNFSRQNTRKHYSDVFVVGGYKWRILIFPKG---NNVDHLSMYLDVSDAASLPYGWS 118

Query: 288 IYAKYILSVYNRVDRKYIDKPARQRWFDNTTYGGGFHEFLARKDL----SNYLDGDVLYL 347
            YA++ L+V N++  +Y  +   Q  F+      GF  F+   +L      YL  D + +
Sbjct: 119 RYAQFSLAVVNQIHTRYTVRKETQHQFNARESDWGFTSFMPLSELYDPSRGYLVNDTVLV 178

Query: 348 KARIVAMSTVE 355
           +A +     ++
Sbjct: 179 EAEVAVRKVLD 186

BLAST of Lag0019844 vs. ExPASy Swiss-Prot
Match: F4J4A1 (MATH domain and coiled-coil domain-containing protein At3g44800 OS=Arabidopsis thaliana OX=3702 GN=At3g44800 PE=4 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 6.0e-08
Identity = 47/152 (30.92%), Postives = 74/152 (48.68%), Query Frame = 0

Query: 72  YTFRIKSYSVLSEIGMMKCESGDFEIDGYKWKLILYPNGDEIGDDHISLYLAISSNKDHN 131
           +T+ IK++S L        +S  F I G KW L+ YPNG +    ++SLYL   + K   
Sbjct: 6   FTWVIKNFSSLQS---EYIKSDIFVIGGCKWCLLAYPNGKQ-NASYLSLYLDGPTLK--T 65

Query: 132 LPLGWEVKVIFRFFIFDQIKDSYLTIQDGKVRKYSKMKSEHGFSHLLSHIDFNKASNGFL 191
           LP G   ++ FR  + +Q+ ++     +GK R + K     G+  +L     N    GFL
Sbjct: 66  LPCGCRRRIRFRLTVVNQLSENLSRRGEGK-RWFDKKLPLCGYEEVLLLTKLNAKHGGFL 125

Query: 192 VDNCCTFGVEVSLLKPSNKGEILTIIKEPQQL 224
           V+N      EV +L+   K   L + KE Q++
Sbjct: 126 VNNEVKIVAEVDVLEVIGK---LDVSKESQEV 147

BLAST of Lag0019844 vs. ExPASy TrEMBL
Match: A0A6J1JFK1 (uncharacterized protein LOC111486429 OS=Cucurbita maxima OX=3661 GN=LOC111486429 PE=4 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 3.6e-117
Identity = 212/299 (70.90%), Postives = 248/299 (82.94%), Query Frame = 0

Query: 59  QITRSRRIDPPADYTFRIKSYSVLSEIGMMKCESGDFEIDGYKWKLILYPNGDEIGDDHI 118
           +ITR+ R+DPPA+Y F+I SYS LSEIGM KCESG F +DGY WKLIL+PNGD+  +DHI
Sbjct: 18  EITRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYNWKLILFPNGDDKVEDHI 77

Query: 119 SLYLAISSNKDHNLPLGWEVKVIFRFFIFDQIKDSYLTIQDGKVRKYSKMKSEHGFSHLL 178
           SLYLAI+S KD NLPLGWEVKVIFRFF++DQIKD+YLTIQDGK+RKYSKMK+EHGF+  L
Sbjct: 78  SLYLAITS-KD-NLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMRKYSKMKTEHGFTKFL 137

Query: 179 SHIDFNKASNGFLVDNCCTFGVEVSLLKPSNKGEILTIIKEPQQLESSYWSFTSFSTKTD 238
           +H  FN+ASNGFLVDN CTFGVEVS+LK SNKGE LTI KEP QL S +WS +SFS K +
Sbjct: 138 AHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEP-QLGSFFWSISSFSNKNN 197

Query: 239 ESYVSYPINVKGRKWRIKIYPNGFSSGKTTHLSLYLKLDSSNTIPVGKKIYAKYILSVYN 298
           E Y S P  VKGRKWR+ IYPNGF SGKT+H+SLYLKLDSS TIP GKKIYAK+ LSVYN
Sbjct: 198 EFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPTGKKIYAKFFLSVYN 257

Query: 299 RVDRKYIDKPARQRWFDNTTYGGGFHEFLARKDLS-NYLDGDVLYLKARIVAMSTVEEF 357
              +KY+D+ + + WFDNT YG GFHEFL+R DL  ++L  DV YLKARIVAMS V++F
Sbjct: 258 FGAKKYVDQ-SNEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYLKARIVAMSVVQDF 312

BLAST of Lag0019844 vs. ExPASy TrEMBL
Match: A0A5A7TNH7 (Ubiquitin carboxyl-terminal hydrolase 12-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00430 PE=4 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 6.2e-117
Identity = 204/295 (69.15%), Postives = 250/295 (84.75%), Query Frame = 0

Query: 62  RSRRIDPPADYTFRIKSYSVLSEIGMMKCESGDFEIDGYKWKLILYPNGDEIGDDHISLY 121
           +SRR+DPP+ Y F+I SYSVLSEI M KCESGDFE+DGY WKLILYPNG+   +DHISL+
Sbjct: 22  KSRRVDPPSHYAFQIDSYSVLSEIEMKKCESGDFEVDGYMWKLILYPNGNGEVEDHISLF 81

Query: 122 LAISSNKDHNLPLGWEVKVIFRFFIFDQIKDSYLTIQDGKVRKYSKMKSEHGFSHLLSHI 181
           LAIS+N ++NLPLGWEV+VIFRFFIFDQI+D+YLTIQDGK+RKYSKMKSEHGF+HL+SH 
Sbjct: 82  LAISTNDNNNLPLGWEVRVIFRFFIFDQIRDNYLTIQDGKMRKYSKMKSEHGFTHLISHN 141

Query: 182 DFNKASNGFLVDNCCTFGVEVSLLKPSNKGEILTIIKEPQQLESSYWSFTSFSTKTDESY 241
            F+KASNGFLV+N CTFGVEVS+LK SNKGE LTI +EPQQ ++ +W+  SFS KT++SY
Sbjct: 142 VFSKASNGFLVNNSCTFGVEVSILKASNKGERLTIFREPQQ-DTYFWTLYSFSAKTNQSY 201

Query: 242 VSYPINVKGRKWRIKIYPNGFSSGKTTHLSLYLKLDSSNTIPVGKKIYAKYILSVYNRVD 301
           +S P NVKGRKWR++IYPNG S GKT+H+SLYLKLD+S TIP+GKKIYAK++L VYN   
Sbjct: 202 ISEPFNVKGRKWRMEIYPNGNSLGKTSHISLYLKLDNSETIPLGKKIYAKFLLGVYNFSA 261

Query: 302 RKYIDKPARQRWFDNTTYGGGFHEFLARKDLSNYLDGDVLYLKARIVAMSTVEEF 357
           +KYIDK + + WF    +G GF EFL+R+++S +   D  YLKARIVAMSTV+EF
Sbjct: 262 KKYIDK-SYEHWFKTPGHGDGFDEFLSRREISTHSQNDAFYLKARIVAMSTVDEF 314

BLAST of Lag0019844 vs. ExPASy TrEMBL
Match: A0A1S3BYG6 (uncharacterized protein LOC103494460 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494460 PE=4 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 6.2e-117
Identity = 204/295 (69.15%), Postives = 250/295 (84.75%), Query Frame = 0

Query: 62  RSRRIDPPADYTFRIKSYSVLSEIGMMKCESGDFEIDGYKWKLILYPNGDEIGDDHISLY 121
           +SRR+DPP+ Y F+I SYSVLSEI M KCESGDFE+DGY WKLILYPNG+   +DHISL+
Sbjct: 22  KSRRVDPPSHYAFQIDSYSVLSEIEMKKCESGDFEVDGYMWKLILYPNGNGEVEDHISLF 81

Query: 122 LAISSNKDHNLPLGWEVKVIFRFFIFDQIKDSYLTIQDGKVRKYSKMKSEHGFSHLLSHI 181
           LAIS+N ++NLPLGWEV+VIFRFFIFDQI+D+YLTIQDGK+RKYSKMKSEHGF+HL+SH 
Sbjct: 82  LAISTNDNNNLPLGWEVRVIFRFFIFDQIRDNYLTIQDGKMRKYSKMKSEHGFTHLISHN 141

Query: 182 DFNKASNGFLVDNCCTFGVEVSLLKPSNKGEILTIIKEPQQLESSYWSFTSFSTKTDESY 241
            F+KASNGFLV+N CTFGVEVS+LK SNKGE LTI +EPQQ ++ +W+  SFS KT++SY
Sbjct: 142 VFSKASNGFLVNNSCTFGVEVSILKASNKGERLTIFREPQQ-DTYFWTLYSFSAKTNQSY 201

Query: 242 VSYPINVKGRKWRIKIYPNGFSSGKTTHLSLYLKLDSSNTIPVGKKIYAKYILSVYNRVD 301
           +S P NVKGRKWR++IYPNG S GKT+H+SLYLKLD+S TIP+GKKIYAK++L VYN   
Sbjct: 202 ISEPFNVKGRKWRMEIYPNGNSLGKTSHISLYLKLDNSETIPLGKKIYAKFLLGVYNFSA 261

Query: 302 RKYIDKPARQRWFDNTTYGGGFHEFLARKDLSNYLDGDVLYLKARIVAMSTVEEF 357
           +KYIDK + + WF    +G GF EFL+R+++S +   D  YLKARIVAMSTV+EF
Sbjct: 262 KKYIDK-SYEHWFKTPGHGDGFDEFLSRREISTHSQNDAFYLKARIVAMSTVDEF 314

BLAST of Lag0019844 vs. ExPASy TrEMBL
Match: A0A6J1FUL4 (speckle-type POZ protein-like A OS=Cucurbita moschata OX=3662 GN=LOC111447016 PE=4 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 2.4e-116
Identity = 209/299 (69.90%), Postives = 250/299 (83.61%), Query Frame = 0

Query: 59  QITRSRRIDPPADYTFRIKSYSVLSEIGMMKCESGDFEIDGYKWKLILYPNGDEIGDDHI 118
           +ITR+RR +PPA+YTF+I SYS LSEIGM KCESG+F +DG+ WKLILYPNGD+  +DHI
Sbjct: 18  EITRTRRAEPPANYTFQINSYSTLSEIGMKKCESGNFAVDGFNWKLILYPNGDDEVEDHI 77

Query: 119 SLYLAISSNKDHNLPLGWEVKVIFRFFIFDQIKDSYLTIQDGKVRKYSKMKSEHGFSHLL 178
           SLYLAI+S KD NLPLGWEVKVIFRFF++D+IKD+YLT+QDGK+RKYSKMKSEHGF+  L
Sbjct: 78  SLYLAITS-KD-NLPLGWEVKVIFRFFVYDRIKDNYLTVQDGKMRKYSKMKSEHGFTKFL 137

Query: 179 SHIDFNKASNGFLVDNCCTFGVEVSLLKPSNKGEILTIIKEPQQLESSYWSFTSFSTKTD 238
           +H  FN+ASNGFLVDN CTFGVEVS+LK SNKGE LTI KEP QL S +WS +SFS KT+
Sbjct: 138 AHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEP-QLGSFFWSISSFSNKTN 197

Query: 239 ESYVSYPINVKGRKWRIKIYPNGFSSGKTTHLSLYLKLDSSNTIPVGKKIYAKYILSVYN 298
           E Y+S P  VKGRKWR+ IYPNG SSGK +H+SLYLKLDSS T+P GKKIYAK+ LSVY+
Sbjct: 198 EFYLSEPFTVKGRKWRLMIYPNGLSSGKMSHISLYLKLDSSETVPTGKKIYAKFFLSVYD 257

Query: 299 RVDRKYIDKPARQRWFDNTTYGGGFHEFLARKDL-SNYLDGDVLYLKARIVAMSTVEEF 357
              ++Y+D+ + + WFDNTTYG GFHEFL+R DL  ++L  D  YLKARI AMS VE+F
Sbjct: 258 FGAKRYVDQ-SNEHWFDNTTYGYGFHEFLSRNDLRPHHLQNDGFYLKARIAAMSVVEDF 312

BLAST of Lag0019844 vs. ExPASy TrEMBL
Match: A0A0A0KUE1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G023030 PE=4 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 3.1e-116
Identity = 204/295 (69.15%), Postives = 248/295 (84.07%), Query Frame = 0

Query: 62  RSRRIDPPADYTFRIKSYSVLSEIGMMKCESGDFEIDGYKWKLILYPNGDEIGDDHISLY 121
           +SRR+DPP+ Y F+I SYSVLS+I M KCESGDFE+DGYKWKLILYPNG+E  +DHISL+
Sbjct: 23  KSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDGYKWKLILYPNGNEEVEDHISLF 82

Query: 122 LAISSNKDHNLPLGWEVKVIFRFFIFDQIKDSYLTIQDGKVRKYSKMKSEHGFSHLLSHI 181
           LA+S+N D+NLPLGWE++VIFRFFIFDQI+D+YLTIQDGK+RKYSKMKSEHGF+HL+SH 
Sbjct: 83  LAVSTN-DNNLPLGWELRVIFRFFIFDQIRDNYLTIQDGKMRKYSKMKSEHGFTHLISHN 142

Query: 182 DFNKASNGFLVDNCCTFGVEVSLLKPSNKGEILTIIKEPQQLESSYWSFTSFSTKTDESY 241
            FNKAS+GFLV NCCTFGVEVS+LK SNKGE LTI+KEPQQ ++ +W+  SFS      Y
Sbjct: 143 VFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEPQQ-DTYFWTLYSFSALKQPFY 202

Query: 242 VSYPINVKGRKWRIKIYPNGFSSGKTTHLSLYLKLDSSNTIPVGKKIYAKYILSVYNRVD 301
           +S P NVKGRKWR+++YP+G S GKT+H+SLYLKLDSS TIP+GKKIYAK+IL VYN   
Sbjct: 203 ISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSETIPLGKKIYAKFILGVYNFSA 262

Query: 302 RKYIDKPARQRWFDNTTYGGGFHEFLARKDLSNYLDGDVLYLKARIVAMSTVEEF 357
           +KYIDK + + W+    +G GF EFL+RK++S +   D  YLKARIVAMSTVEEF
Sbjct: 263 KKYIDK-SYEHWYKTPGHGNGFDEFLSRKEISTHSQNDAFYLKARIVAMSTVEEF 314

BLAST of Lag0019844 vs. TAIR 10
Match: AT3G17380.1 (TRAF-like family protein )

HSP 1 Score: 183.7 bits (465), Expect = 2.5e-46
Identity = 96/274 (35.04%), Postives = 161/274 (58.76%), Query Frame = 0

Query: 59  QITRSRRIDPPADYTFRIKSYSVLSEIGMMKCESGDFEIDGYKWKLILYPNGDEIGD--D 118
           +I +S    PP  Y  +I+S+S+L++  + + E+  FE  GYKWKL+LYPNG++  +  D
Sbjct: 9   EIIKSISDAPPTHYMVKIESFSLLTKHAIERYETESFEAGGYKWKLVLYPNGNKSKNTKD 68

Query: 119 HISLYLAISSNKDHNLPLGWEVKVIFRFFIFDQIKDSYLTIQDGKVRKYSKMKSEHGFSH 178
           H+S+YL+++ +   +L  GWEV  +FR ++ DQ KD+YL +Q G  R++  +K E GF  
Sbjct: 69  HVSVYLSLADSS--SLSPGWEVYAVFRLYLLDQNKDNYLILQ-GNERRFHSVKREWGFDK 128

Query: 179 LLSHIDFNKASNGFLVDNCCTFGVEVSLLKP--SNKGEILTIIKEPQQLESSYWSFTSFS 238
            +    F+ ASNG+L+++ C FG +V + K   S +GE L++IK+        W   +FS
Sbjct: 129 FIPTGTFSDASNGYLMEDTCMFGADVFVSKERRSGRGECLSMIKDATS-SKHVWKIENFS 188

Query: 239 TKTDESYVSYPINVKGRKWRIKIYPNGFSSGKTTHLSLYLKLDSSNTIPVGKKIYAKYIL 298
               ESY S       RKW+I+ YP G   G  THLS+YL L    TI  G KI+ ++ +
Sbjct: 189 KLDKESYDSNAFFAGDRKWKIEFYPTGTKQGTGTHLSIYLTLVDPETISDGTKIFVEFTI 248

Query: 299 SVYNRVDRKYIDKPARQRWFDNTTYGGGFHEFLA 329
            +++++  ++I      +WF  ++   G+ ++++
Sbjct: 249 RIFDQLQGRHIAGKV-TKWFSRSSSEHGWVKYVS 277

BLAST of Lag0019844 vs. TAIR 10
Match: AT5G26280.1 (TRAF-like family protein )

HSP 1 Score: 149.4 bits (376), Expect = 5.3e-36
Identity = 94/298 (31.54%), Postives = 146/298 (48.99%), Query Frame = 0

Query: 60  ITRSRRIDPPADYTFRIKSYSVLSEIGMMKCESGDFEIDGYKWKLILYPNG--DEIGDDH 119
           +TR  R + P+     I S+SV+   G    ES  FE  GYKW+L+LY NG  ++ G+DH
Sbjct: 50  VTRELREERPSSKIVTITSFSVIKGRG-EPYESSVFEAAGYKWRLVLYVNGNKNDGGNDH 109

Query: 120 ISLYLAISSNKDHNLPLGWEVKVIFRFFIFDQIKDSYLTIQDGKVRKYSKMKSEHGFSHL 179
           ISLY  I   + ++LPLGWEV V  + F+ +     YLT+ DG V++Y+  K E GF  L
Sbjct: 110 ISLYARI--EETNSLPLGWEVNVDLKLFVHNGKLHKYLTVTDGLVKRYNNAKKEWGFGQL 169

Query: 180 LSHIDFNKASNGFLVDNCCTFGVEVSLLKPSNKGEILTIIKEPQQLESSYWSFTSFSTKT 239
           +    F  A+ G+L  +  +FG E+ ++KP+ + E +T I  P       W    FST  
Sbjct: 170 IPRSTFYNANEGYLDQDTGSFGAEIFIVKPAQQQEKVTFISNPPN-NVFTWKILRFSTLE 229

Query: 240 DESYVSYPINVKGRKWRIKIYPNGFSSGKTTHLSLYLKLDSSNTIPVGKKIYAKYILSVY 299
           D+ Y S    V+ R WR+   P G   G+   L ++L         V    +    L + 
Sbjct: 230 DKFYYSDDFLVEDRYWRLGFNPKGDGGGRPHALPIFLFAQGHKANAVATNTWGAVNLRLK 289

Query: 300 NRVDRKYIDKPARQRWFDNTTYGGGFHEFLARKDLSN----YLDGDVLYLKARIVAMS 352
           N+    +    +   +   + YG G +  +   DL++    YL  D +  +A +V +S
Sbjct: 290 NQRSTNHRQIYSAAWYPIGSGYGVGVNNIILLADLNDASKGYLVNDAIIFEAEMVKVS 343

BLAST of Lag0019844 vs. TAIR 10
Match: AT5G26260.1 (TRAF-like family protein )

HSP 1 Score: 143.7 bits (361), Expect = 2.9e-34
Identity = 90/297 (30.30%), Postives = 142/297 (47.81%), Query Frame = 0

Query: 61  TRSRRIDPPADYTFRIKSYSVLSEIGMMKCESGDFEIDGYKWKLILYPNGDEIG--DDHI 120
           TR  R + P+     I S+SV+ + G    ES  FE  GYKW+L+LY  G+  G  ++HI
Sbjct: 52  TRELRDERPSSKIVTITSFSVIKDRG-EPYESSIFEAAGYKWRLVLYVKGNPKGGINNHI 111

Query: 121 SLYLAISSNKDHNLPLGWEVKVIFRFFIFDQIKDSYLTIQDGKVRKYSKMKSEHGFSHLL 180
           SLY  I   +   LP GWEV V  + F+ ++    YL++ DG V++Y+  K E GF+ L+
Sbjct: 112 SLYARIEETE--TLPRGWEVNVDLKLFVHNRKLKKYLSVTDGTVKRYNDAKKEWGFTQLI 171

Query: 181 SHIDFNKASNGFLVDNCCTFGVEVSLLKPSNKGEILTIIKEPQQLESSYWSFTSFSTKTD 240
           S   F  A+ G+LV +  +FG E+ ++ P+ K E +T I  P       W    FST  D
Sbjct: 172 SLPTFYNANEGYLVQDTASFGAEIFIVNPTEKQEKVTFISNPPD-NVFTWKILRFSTLED 231

Query: 241 ESYVSYPINVKGRKWRIKIYPNGFSSGKTTHLSLYLKLDSSNTIPVGKKIYAKYILSVYN 300
           + Y S    V  R WR+   P G   G+   L ++L         V    +    L + N
Sbjct: 232 KFYYSDDFLVGDRYWRLGFNPKGSGGGRPHALPIFLYAQGHKANAVVTNTWGAVNLRLKN 291

Query: 301 RVDRKYIDKPARQRWFDNTTYGGGFHEFLARKDLSN----YLDGDVLYLKARIVAMS 352
           +    +    +   +   + YG G +  +   +L +    Y+  D +  +A +V +S
Sbjct: 292 QRSSNHKQLYSAAWYPIRSDYGVGVNNIILMSELKDASKGYMVNDAIIFEAEMVKVS 344

BLAST of Lag0019844 vs. TAIR 10
Match: AT4G09770.1 (TRAF-like family protein )

HSP 1 Score: 141.4 bits (355), Expect = 1.4e-33
Identity = 89/294 (30.27%), Postives = 147/294 (50.00%), Query Frame = 0

Query: 65  RIDPPADYTFRIKSYSVLSEIGMMKCESGDFEIDGYKWKLILYPNG--DEIGDDHISLYL 124
           R + P+ Y  ++  +S + +      ES DF++ G+KW+LI YP G  +E G DH+S+Y 
Sbjct: 5   REEAPSSYLMKLVGFSEV-KFSHQPYESADFDVGGHKWRLIFYPAGKLEEGGKDHVSIYA 64

Query: 125 AISSNKDHNLPLGWEVKVIFRFFIFDQIKDSYLTIQDGKVRKYSKMKSEHGFSHLLSHID 184
            I      N+    ++    +FFI++     Y   QDG ++ Y+K K E GF+ +L    
Sbjct: 65  RI-----ENVGASMQIDAELKFFIYNHNNKQYSVFQDGTMKHYNKEKKECGFAQMLLFSK 124

Query: 185 FNKASNGFLVDNCCTFGVEVSLLKPSNKGEILTIIKEPQQLESSYWSFTSFSTKTDES-Y 244
           FN   NG++  N C  GVE+ ++KP  K E +   + P + + + W  + FS   D+  Y
Sbjct: 125 FNDPKNGYIDGNACIVGVEIFVIKPIEKVERVVFTQNPPENKFT-WKISHFSYIGDKRYY 184

Query: 245 VSYPINVKGRKWRIKIYPNGFSSGKTTHLSLYLKLDSSNTIPVGKKIYAKYILSVYNRVD 304
            S    V  RKWR+KI P G    K   LS+Y++  +     V    YAK  L + N+ +
Sbjct: 185 YSDEFVVGDRKWRLKISPKG--DKKVRALSVYVQAMAYLPNAVASSTYAKLRLRLLNQKN 244

Query: 305 RKYIDKPARQRWFDNTTYGGGFHEFLARKDLSN----YLDGDVLYLKARIVAMS 352
             +I+K     +      G G  E ++ +DL++    YL  D + L+  ++ +S
Sbjct: 245 SNHIEKRVFHFYSRENGDGSGISELISVEDLNDESKGYLVEDSIVLETTLLWVS 289

BLAST of Lag0019844 vs. TAIR 10
Match: AT4G09770.2 (TRAF-like family protein )

HSP 1 Score: 141.4 bits (355), Expect = 1.4e-33
Identity = 89/294 (30.27%), Postives = 147/294 (50.00%), Query Frame = 0

Query: 65  RIDPPADYTFRIKSYSVLSEIGMMKCESGDFEIDGYKWKLILYPNG--DEIGDDHISLYL 124
           R + P+ Y  ++  +S + +      ES DF++ G+KW+LI YP G  +E G DH+S+Y 
Sbjct: 5   REEAPSSYLMKLVGFSEV-KFSHQPYESADFDVGGHKWRLIFYPAGKLEEGGKDHVSIYA 64

Query: 125 AISSNKDHNLPLGWEVKVIFRFFIFDQIKDSYLTIQDGKVRKYSKMKSEHGFSHLLSHID 184
            I      N+    ++    +FFI++     Y   QDG ++ Y+K K E GF+ +L    
Sbjct: 65  RI-----ENVGASMQIDAELKFFIYNHNNKQYSVFQDGTMKHYNKEKKECGFAQMLLFSK 124

Query: 185 FNKASNGFLVDNCCTFGVEVSLLKPSNKGEILTIIKEPQQLESSYWSFTSFSTKTDES-Y 244
           FN   NG++  N C  GVE+ ++KP  K E +   + P + + + W  + FS   D+  Y
Sbjct: 125 FNDPKNGYIDGNACIVGVEIFVIKPIEKVERVVFTQNPPENKFT-WKISHFSYIGDKRYY 184

Query: 245 VSYPINVKGRKWRIKIYPNGFSSGKTTHLSLYLKLDSSNTIPVGKKIYAKYILSVYNRVD 304
            S    V  RKWR+KI P G    K   LS+Y++  +     V    YAK  L + N+ +
Sbjct: 185 YSDEFVVGDRKWRLKISPKG--DKKVRALSVYVQAMAYLPNAVASSTYAKLRLRLLNQKN 244

Query: 305 RKYIDKPARQRWFDNTTYGGGFHEFLARKDLSN----YLDGDVLYLKARIVAMS 352
             +I+K     +      G G  E ++ +DL++    YL  D + L+  ++ +S
Sbjct: 245 SNHIEKRVFHFYSRENGDGSGISELISVEDLNDESKGYLVEDSIVLETTLLWVS 289