Lag0019782 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0019782
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRibonuclease T(2)
Locationchr5: 45448567 .. 45452326 (+)
RNA-Seq ExpressionLag0019782
SyntenyLag0019782
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTTTGTCGTTGAAAGCGGCGGCGGCGGCGGTGCTTGTGGCTGTTGCCTTTGTTGCTCAGCTGCAGGCGGCGAGGTTTGGGGCGGAGGAGAATGAATTTGACTACTTTGTGTTATCGCTGCAATGGTCAGGCACTTCCTGCAGAAGCAATAGCGTGTGTTGCCCAGAAAGTGGCTGCTGCGCTGGGTGAGATCAGATCACTTCTATGTTTTTTTTAATGACTTTTACGGTGTGTTTTATTTCGAAAAAAAAAATCATTTTGAATTAAAATGACTTATTAGAAAATGAGATTGATGAATAAATCATTTTGAGACAAACACTTGAAACCAACTTCTTTAAAAGATTTTTTAAGTATATAATAGCTTGTGTATAAAAACACTCGTGAGGAAATATAACCAAACATATGGGTGTTTAAATTCTAAACAATTTTTATACAAAATGTTTTTAAAAAAAATGCATTCTTGAAAAACATTTAAATTGGAATGCATTCCAAACGGACCCTAGTTGTTGTTGGTTTTTCGTTTTTTTTTTCTTTTTCATGTTGTGTTGTGCTAATTAAGATTTTTGTGTGTTTATTCTTTCTCTAGAGCTGACACTCCGACTCAATTTACAATTCGTAAGTGTGAAAAAATCTGAGCTCCCTTAGCTTTGGATTTTGCTTTTCATTTGGATGCATTGTTAAATAAATTGATGACCAAAATTTGGCTGCATATCTGTATGTAGAAATCTTCCTATTTACTACATTAAAATCTTGACAATTAAGAAAGAGGAAAGGAGAAAATGAGAAAACTTTTTTATCTCTCCTAGTTGTCAAAATTTTAATGGTGATATCCAGAGAGATTCCTATGTCATGGAGCCAAAAATTTTCCTAAATTGAGTTTAAATCATCTAAGAGTTTTTTCTTCATTGGTGCAGATGGGCTGTGGCCTGTGAAAGAATGGGGTAAAAATCCTGAATGTTGTACTGATGCCGTATATAATGAAACTGAGGTCTCTCTCTCAAATTTAATGATGCAACCACTTGAAAACTATGCTAAAAAGGGGTTTAAAGTGTTAAAAAAATTCATCATTTTTAAGCGAAAACCTATTTGACACAAACCTATGAAAGTCGAGGAACTATTATACACAAAATTGAAACATTAAAAAGACTTTGTTAACTAAACCTTCTTAAAGTATATGGATATATGGACTAGATAAATCAAATCTTAAAAAAGTTTATCAACTTAGCTATAAATTTAAAATTTTAGGAGACAAACATGTAATCTATTAACCTAAAAAGTTAAGAATACTCTAAAGTACATTAAAAGATTAGGGAAAAATACATTGTTGGTCCCTGAAGTTTGGGTAAGGTTTCAATTTTGGTTCGTAAGTTTTCAAACTCGACATTATAACCCTTGAGGTTTGGATTCTGTTACATTATTGGTCTCCCCGTCCAATTTTCTGTTAAATCCTAACAGATTACTGACGTGACACCATTTTTTATTAAAAAAATATATTTTTTTATAATTTTTAAAAATAAAATATATTAAAATAATAAAAATATCTTCTTCTTCACCTTCACCATAATCCTTCTTCTTCTTCACTTTCACTTTCACCACCACATAACCCTAAGCCCTACCGCCAGCAGCCACCGCAAAATGAAACGTAATCCAAACCTAAGGAACCAAAAGTGTACTTTTTTCCAAAAGATTAAATATACCTTAAATGCTCATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTCCTTGCTAACACATATTATTATTTCATGATGTTAAAAATATGATAATGGCATATGTCAAACCAAAAGTATTGACTCTTAATTACCTTGACAACACTTGTAGATTAATCCCTTGAAGGAGGAACTTGACAAGTATTGGCCAACTTATCAATGTGGTTCTCAACCAACCTGCAATGGTAGAACAGGATCCTTGTGGGCTGAAGAGGTTCTAAATTCTCAATCTCTCCTCTCCTTTAATTTTCTTCCTTAATTACCTCATTATCAAAATTTTAGACTAACCCTAATTTCTCTCATCTTTTGTCTCTCTCTTGTTGCCATTGCTGCAGTATGGTAAGCCACTTCGACCCGTACGTTTCTTTGTTTTATTATCTACACCCTAAAAATATTTTCAAAATCCAGAGAAAAAAGTAAATTTTTAAGAACTTGTTTTTGTTTCTGAAATTTGACTAACAGTTTAAATGTTTTTTGAGCATTCAAAAACCAAAAACCAAGGCCCCATTTGATAACGTCATTTGGTTTTTTGTTTTTGGTTTTTTTAAATTATTAAGCTAATAAACATTACTCCCACTTAAGAGTTTTTTTGTTTTTTCATCTACCTTGTTTCCATGTTTTCAAAAACCAAACCGATTTTTTAAAACTAAAAAAAGTCGTTTTCAAAAACTTCGTTTTTGTTTTTGGAATTTGACTAGGAATTCAAATGCATCTTTAACAAAGATATGAAAACCATAGCAAGTAAAATGGAAGAAAACAAACATATTTTTCAAAGACCAAATGGTTATCAAACGGGACACAAATAATGTTTACGAAACGGATCTAATTTTGCAGAGAGGCATGGAACTTGCGCATCTCATGTAATGAAGAGCGAATTTGAATACTTCGATCAATCCCTCAATCTGTTCTTAGAGTCCAATGTCAATGTAAGCTTTTATAAACTTTTTTTTTTTCTTTACAACGAAGTACGCTTATGTTGGCTAAGTTTTTATAAACTTGGTTATCGATTTTCAACCGTTAAATTAAAATGAAAACTTTGGTGCAATATATATATTTATAAAACCTTTTTTTTTTTTTTTTTGTTACTTAGAAAGCTTTGAAGAATGCAAATATCGTAGCTTCGGATACTGTAACGTATTCCAAAGATGAGGTGATCCATGTAATTGAGGAGGCTGTTTTACGAGGAAAGCCAGAGCTTATATGTGATGATGATGATGAGATTGTCAAGGAACTTTGGCTATGCTATGATAAACGACTAGTGGTATTACTTTGTGTTTGTAAAATTAAGGTCCCGTTTGATAACTATTTGGTTTTTTAAAATCATGAGGTAAATTGATACTAAAACAAAGAAACTTATAGGTGGGAGTAGGTGTTGTATAAACTTAATTTTCAAAAACCAAAAACAAAAAGCTAAATAATTATCAAACGGGGCCTAATTAATTAATATTCCTAACTGTGATAGATTGATGATCAAAAACTAAATGTTTACTCTTTATATATGCAGCCTCGTGATTGTGATCCTCCTACTATAGAATCATGCTCCGACTCTATTAAGCTCCCGCTAAAACCTCCACCATCGGGTAAGTTTCTTTCTATTCTAAGGATCTAAATTCATCGAGCCATAGTGACGGTTGGTCAGTTTTAAGCTCAGAGAGAAAAGGGAGAAAACCCAAATGGTTATCAAACGAGACCTTAGTTTATAGATACCCTTATAATTCTTAGATAATAAAGTTTTCTTGGTTAAATCACCCTGAATACATAGACTATAATAATTAAAGAGAGAAATGAAGTTGAAGAGATTGACACACAAAATACGTCTGGTGTGTTTTGGTACTATATAAGATCATCGCTAAACCCAAAAGCTTAGATTAAATAACTAATCAATATATTTATTTCATTGGCAATTGGTTTTGAGATGTAAGCCCATATAATATAATATTATTGCAGAGACATGTTGCGGGGCTAATGGAGCAATTTCATGGATGTAG

mRNA sequence

ATGGCTTCTTTGTCGTTGAAAGCGGCGGCGGCGGCGGTGCTTGTGGCTGTTGCCTTTGTTGCTCAGCTGCAGGCGGCGAGGTTTGGGGCGGAGGAGAATGAATTTGACTACTTTGTGTTATCGCTGCAATGGTCAGGCACTTCCTGCAGAAGCAATAGCGTGTGTTGCCCAGAAAGTGGCTGCTGCGCTGGAGCTGACACTCCGACTCAATTTACAATTCATGGGCTGTGGCCTGTGAAAGAATGGGGTAAAAATCCTGAATGTTGTACTGATGCCGTATATAATGAAACTGAGATTAATCCCTTGAAGGAGGAACTTGACAAGTATTGGCCAACTTATCAATGTGGTTCTCAACCAACCTGCAATGGTAGAACAGGATCCTTGTGGGCTGAAGAGTATGGTAAGCCACTTCGACCCGAATTCAAATGCATCTTTAACAAAGATATGAAAACCATAGCAAAGAGGCATGGAACTTGCGCATCTCATGTAATGAAGAGCGAATTTGAATACTTCGATCAATCCCTCAATCTGTTCTTAGAGTCCAATGTCAATAAAGCTTTGAAGAATGCAAATATCGTAGCTTCGGATACTGTAACGTATTCCAAAGATGAGGTGATCCATGTAATTGAGGAGGCTGTTTTACGAGGAAAGCCAGAGCTTATATGTGATGATGATGATGAGATTGTCAAGGAACTTTGGCTATGCTATGATAAACGACTAGTGCCTCGTGATTGTGATCCTCCTACTATAGAATCATGCTCCGACTCTATTAAGCTCCCGCTAAAACCTCCACCATCGGAGACATGTTGCGGGGCTAATGGAGCAATTTCATGGATGTAG

Coding sequence (CDS)

ATGGCTTCTTTGTCGTTGAAAGCGGCGGCGGCGGCGGTGCTTGTGGCTGTTGCCTTTGTTGCTCAGCTGCAGGCGGCGAGGTTTGGGGCGGAGGAGAATGAATTTGACTACTTTGTGTTATCGCTGCAATGGTCAGGCACTTCCTGCAGAAGCAATAGCGTGTGTTGCCCAGAAAGTGGCTGCTGCGCTGGAGCTGACACTCCGACTCAATTTACAATTCATGGGCTGTGGCCTGTGAAAGAATGGGGTAAAAATCCTGAATGTTGTACTGATGCCGTATATAATGAAACTGAGATTAATCCCTTGAAGGAGGAACTTGACAAGTATTGGCCAACTTATCAATGTGGTTCTCAACCAACCTGCAATGGTAGAACAGGATCCTTGTGGGCTGAAGAGTATGGTAAGCCACTTCGACCCGAATTCAAATGCATCTTTAACAAAGATATGAAAACCATAGCAAAGAGGCATGGAACTTGCGCATCTCATGTAATGAAGAGCGAATTTGAATACTTCGATCAATCCCTCAATCTGTTCTTAGAGTCCAATGTCAATAAAGCTTTGAAGAATGCAAATATCGTAGCTTCGGATACTGTAACGTATTCCAAAGATGAGGTGATCCATGTAATTGAGGAGGCTGTTTTACGAGGAAAGCCAGAGCTTATATGTGATGATGATGATGAGATTGTCAAGGAACTTTGGCTATGCTATGATAAACGACTAGTGCCTCGTGATTGTGATCCTCCTACTATAGAATCATGCTCCGACTCTATTAAGCTCCCGCTAAAACCTCCACCATCGGAGACATGTTGCGGGGCTAATGGAGCAATTTCATGGATGTAG

Protein sequence

MASLSLKAAAAAVLVAVAFVAQLQAARFGAEENEFDYFVLSLQWSGTSCRSNSVCCPESGCCAGADTPTQFTIHGLWPVKEWGKNPECCTDAVYNETEINPLKEELDKYWPTYQCGSQPTCNGRTGSLWAEEYGKPLRPEFKCIFNKDMKTIAKRHGTCASHVMKSEFEYFDQSLNLFLESNVNKALKNANIVASDTVTYSKDEVIHVIEEAVLRGKPELICDDDDEIVKELWLCYDKRLVPRDCDPPTIESCSDSIKLPLKPPPSETCCGANGAISWM
Homology
BLAST of Lag0019782 vs. NCBI nr
Match: XP_022922895.1 (ribonuclease 2-like [Cucurbita moschata])

HSP 1 Score: 234.2 bits (596), Expect = 1.4e-57
Identity = 125/286 (43.71%), Postives = 168/286 (58.74%), Query Frame = 0

Query: 1   MASLSLKA-----AAAAVLVAVAFVAQLQAAR--FGAEENEFDYFVLSLQWSGTSCRSNS 60
           MAS S+K+     A A  L+ +  VA + AA    G E+ EFDYFVL+LQW  TSC++  
Sbjct: 1   MASFSIKSNPMVVAPAVFLLVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTG 60

Query: 61  VCCPESGCCAGADTPTQFTIHGLWPVKEWGKNPECCTDAVYNETEINPLKEELDKYWPTY 120
            CCP +GCC GAD+PT+FTIHGLWP       P CCTDAV+++ EI  LKE+++KYWPTY
Sbjct: 61  KCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFSQNEITLLKEDIEKYWPTY 120

Query: 121 QCGSQPTCNGRTGSLWAEEYGKPLRPEFKCIFNKDMKTIAKRHGTCASHVMKSEFEYFDQ 180
           +CG+   C+G  GS WA EY                    ++HGTCA+ V+  EF+YF  
Sbjct: 121 RCGTTSNCHGTKGSFWAHEY--------------------EKHGTCAAPVIVGEFDYFLT 180

Query: 181 SLNLFLESNVNKALKNANIVASDTVTYSKDEVIHVIEEAVLRGKPELICDDDDEIVKELW 240
           +L +F + NV KAL +A  VAS+T  Y  ++V+  ++ A     P++ C      VKELW
Sbjct: 181 TLTIFSKYNVTKALSDAGFVASNTEKYPLEDVVDAVKNAT-NSTPKISCAKKG-AVKELW 240

Query: 241 LCYDKRLVPRDCDPPTIESCSDSIKLPLKPPPSETCCGANGAISWM 280
           +C+DK+  PRDCD     SC + IKLP   P         GAI W+
Sbjct: 241 ICFDKKFEPRDCD--LANSCPEFIKLPTYEPQDV----VEGAIPWI 258

BLAST of Lag0019782 vs. NCBI nr
Match: XP_022140986.1 (ribonuclease 2-like [Momordica charantia])

HSP 1 Score: 233.0 bits (593), Expect = 3.1e-57
Identity = 118/256 (46.09%), Postives = 160/256 (62.50%), Query Frame = 0

Query: 5   SLKAAAAAVLVAVAFVAQLQAARFGAEENEFDYFVLSLQWSGTSCRSNSVCCPESGCCAG 64
           S   AA A  V V  V QLQ A F  ++ EFDYFVL+LQW G++C++ S CC ++GCC G
Sbjct: 8   SNSVAAVAAAVFVVAVVQLQTAAFAKDQREFDYFVLALQWPGSACKNISYCCQDNGCCRG 67

Query: 65  ADTPTQFTIHGLWPVKEWGKNPECCTDAVYNETEINPLKEELDKYWPTYQCGSQPTCNGR 124
           + TPTQFTIHGLWP  E    P CCTD  +NE EI  L++ L + WP+Y+CGS  +CN +
Sbjct: 68  SQTPTQFTIHGLWPQYEVKGWPSCCTDESFNENEIFVLRKILPEIWPSYRCGSVTSCNNK 127

Query: 125 TGSLWAEEYGKPLRPEFKCIFNKDMKTIAKRHGTCASHVMKSEFEYFDQSLNLFLESNVN 184
            GS WAEEY                    ++HGTCA+ V++ ++ YF  ++ LF   NV 
Sbjct: 128 KGSFWAEEY--------------------EKHGTCAAPVIEGQYNYFSTAIGLFNTHNVT 187

Query: 185 KALKNANIVASDTVTYSKDEVIHVIEEAVLRGKPELICDDDDEIVKELWLCYDKRLVPRD 244
           KAL+NA IVASDTV Y  ++V+  +E A   G P+++C     I+KEL+LC+DK+  PR+
Sbjct: 188 KALENAGIVASDTVKYPLNDVMAAVEFA-FGGTPKIMCGKKG-IIKELYLCFDKKFKPRN 239

Query: 245 CDPPTIESCSDSIKLP 261
           CD    +SC   +KLP
Sbjct: 248 CD--ETKSCPKKVKLP 239

BLAST of Lag0019782 vs. NCBI nr
Match: XP_022140985.1 (ribonuclease 2-like [Momordica charantia])

HSP 1 Score: 231.9 bits (590), Expect = 6.8e-57
Identity = 128/287 (44.60%), Postives = 165/287 (57.49%), Query Frame = 0

Query: 1   MASLSLKA-AAAAVLVAVAFVAQLQAARFGA-------EENEFDYFVLSLQWSGTSCRSN 60
           MAS S+K    AA  V +  V QLQAA F A       E+ EFDYFVL+LQW  TSC + 
Sbjct: 1   MASSSIKGNPTAAATVFLLVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTNP 60

Query: 61  SVCCPESGCCAGADTPTQFTIHGLWPVKEWGKNPECCTDAVYNETEINPLKEELDKYWPT 120
             CCP +GCC GA++PT+FTIHGLWP  E    P CC+DA ++E EI  L +++ KYWPT
Sbjct: 61  GKCCPSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYWPT 120

Query: 121 YQCGSQPTCNGRTGSLWAEEYGKPLRPEFKCIFNKDMKTIAKRHGTCASHVMKSEFEYFD 180
           Y+CG+  TC+   GS WA EY                    ++HGTCA+ V+  EF+YF 
Sbjct: 121 YRCGTTSTCHRTKGSFWAHEY--------------------EKHGTCAAPVIVGEFDYFL 180

Query: 181 QSLNLFLESNVNKALKNANIVASDTVTYSKDEVIHVIEEAVLRGKPELICDDDDEIVKEL 240
            +L +F + NV K L +A  VASDT  Y+  +V+  +++A     P++ C      VKEL
Sbjct: 181 TTLTIFSKYNVTKVLNDAGFVASDTEKYALGDVVAAVQDA-FNATPKISCAKKG-AVKEL 240

Query: 241 WLCYDKRLVPRDCDPPTIESCSDSIKLPLKPPPSETCCGANGAISWM 280
           WLC+DK   PRDCD     SC D IKLP   P  +      GAI WM
Sbjct: 241 WLCFDKTFQPRDCD--LANSCPDFIKLPTYEP--QEANKVEGAIPWM 261

BLAST of Lag0019782 vs. NCBI nr
Match: XP_004137162.1 (ribonuclease 2 [Cucumis sativus])

HSP 1 Score: 228.0 bits (580), Expect = 9.9e-56
Identity = 123/287 (42.86%), Postives = 163/287 (56.79%), Query Frame = 0

Query: 1   MASLSLKAAAAAVLVAVAFVAQLQAARFG--------AEENEFDYFVLSLQWSGTSCRSN 60
           MASLS+KA A   +V V  + QL    FG        +E+ EFDYFVL+LQW G+SC++ 
Sbjct: 1   MASLSIKAKAMVFVVVV--LLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNP 60

Query: 61  SVCCPESGCCAGADTPTQFTIHGLWPVKEWGKNPECCTDAVYNETEINPLKEELDKYWPT 120
             CCP + CC GA++PT+FTIHGLWP       P CCTDA +NE EIN L E++ KYWPT
Sbjct: 61  GKCCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPT 120

Query: 121 YQCGSQPTCNGRTGSLWAEEYGKPLRPEFKCIFNKDMKTIAKRHGTCASHVMKSEFEYFD 180
           Y+CG+  TC+   GS WA EY                    ++HGTCA+ V+  E++YF 
Sbjct: 121 YRCGTTSTCHQTKGSFWAHEY--------------------EKHGTCAAPVIVGEYDYFL 180

Query: 181 QSLNLFLESNVNKALKNANIVASDTVTYSKDEVIHVIEEAVLRGKPELICDDDDEIVKEL 240
            ++ +F + NV K L +A  VAS+T  Y  ++V+  I+       P++ C      VKEL
Sbjct: 181 TTITIFSKYNVTKVLSDAGFVASNTEKYPIEDVVAAIKNEFNNATPKISCAKKG-AVKEL 240

Query: 241 WLCYDKRLVPRDCDPPTIESCSDSIKLPLKPPPSETCCGANGAISWM 280
           WLC+DK   PRDC+ P   SC D IK P   P         GAI W+
Sbjct: 241 WLCFDKSFEPRDCNLP--NSCPDFIKFPTYEPQEPN--KVEGAIPWI 260

BLAST of Lag0019782 vs. NCBI nr
Match: XP_038876700.1 (ribonuclease 2-like [Benincasa hispida])

HSP 1 Score: 227.6 bits (579), Expect = 1.3e-55
Identity = 125/287 (43.55%), Postives = 162/287 (56.45%), Query Frame = 0

Query: 1   MASLSLKAAAAAVLVAVAFVAQLQAARF--------GAEENEFDYFVLSLQWSGTSCRSN 60
           MAS S+KA   A  V V  + QL A  F        G+E+ EFDYFVL+LQW  TSC++ 
Sbjct: 1   MASFSIKAKPMAAPV-VFLLLQLLAVAFGFTAVAPSGSEQREFDYFVLALQWPATSCKNP 60

Query: 61  SVCCPESGCCAGADTPTQFTIHGLWPVKEWGKNPECCTDAVYNETEINPLKEELDKYWPT 120
             CCP +GCC G D+PT+FTIHGLWP       P CCTDA +NE EIN L E++ KYWPT
Sbjct: 61  GKCCPANGCCRGVDSPTEFTIHGLWPQYNDRGWPSCCTDANFNENEINILAEDIQKYWPT 120

Query: 121 YQCGSQPTCNGRTGSLWAEEYGKPLRPEFKCIFNKDMKTIAKRHGTCASHVMKSEFEYFD 180
           Y+CG+  TC+   GS WA EY                    ++HGTCA+ V+  E++YF 
Sbjct: 121 YRCGTTSTCHRTKGSFWAHEY--------------------EKHGTCAAPVIVGEYDYFL 180

Query: 181 QSLNLFLESNVNKALKNANIVASDTVTYSKDEVIHVIEEAVLRGKPELICDDDDEIVKEL 240
            +L +F + NV K L +A  VAS+T  Y  ++V+  I+       P++ C     +VKEL
Sbjct: 181 TTLTIFSKYNVTKVLNDAGFVASNTEKYPIEDVVAAIKNE-FNSTPKITCAKKG-VVKEL 240

Query: 241 WLCYDKRLVPRDCDPPTIESCSDSIKLPLKPPPSETCCGANGAISWM 280
           W+C+DK   PRDCD     SC D IKLP   P         GAI W+
Sbjct: 241 WICFDKSFEPRDCD--LQNSCPDFIKLPTYDPQEPN--KVEGAIPWI 260

BLAST of Lag0019782 vs. ExPASy Swiss-Prot
Match: P42814 (Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 4.0e-44
Identity = 100/264 (37.88%), Postives = 137/264 (51.89%), Query Frame = 0

Query: 8   AAAAAVLVAVAFVAQLQAA---RFGAEENEFDYFVLSLQWSGTSCRSNSVCCPESGCCAG 67
           A+   +L+ VA +A   A         + EFDYF LSLQW GT CR    CC ++ CC G
Sbjct: 2   ASRLCLLLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRG 61

Query: 68  ADTPTQFTIHGLWPVKEWGKNPECCTDAVYNETEINPLKEELDKYWPTYQCGSQPTCNGR 127
           +D PTQFTIHGLWP    G  P CC  + + E EI+ L + L+KYWP+  CGS  +CNG 
Sbjct: 62  SDAPTQFTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGG 121

Query: 128 TGSLWAEEYGKPLRPEFKCIFNKDMKTIAKRHGTCASHVMKSEFEYFDQSLNLFLESNVN 187
            GS W  E+                    ++HGTC+S V   E+ YF  +LNL+L+ NV 
Sbjct: 122 KGSFWGHEW--------------------EKHGTCSSPVFHDEYNYFLTTLNLYLKHNVT 181

Query: 188 KALKNANIVASDTVTYSKDEVIHVIEEAVLRGKPELICDDDDEIVKELWLCYDKRLVPRD 247
             L  A  VAS++  Y    ++  I+ A     PE++C  D   + E+ +C+ K   PRD
Sbjct: 182 DVLYQAGYVASNSEKYPLGGIVTAIQNA-FHITPEVVCKRD--AIDEIRICFYKDFKPRD 241

Query: 248 C----DPPTIESCSDSIKLPLKPP 265
           C    D  + +SC   + LP   P
Sbjct: 242 CVGSQDLTSRKSCPKYVSLPEYTP 242

BLAST of Lag0019782 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.0e-15
Identity = 63/224 (28.12%), Postives = 104/224 (46.43%), Query Frame = 0

Query: 19  FVAQLQAARFGAEENEFDYFVLSLQWSGTSCRSNSVCC-PESGCCAGADTPTQFTIHGLW 78
           F+  LQ     +   +FD+F   LQW G  C S   CC P++G  A       F IHGLW
Sbjct: 6   FILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPA-----ADFGIHGLW 65

Query: 79  PVKEWGKNPECCT-DAVYNETEINPLKEELDKYWPTYQCGSQPTCNGRTGSLWAEEYGKP 138
           P  + G  P+ C  D+ +++  ++ L  +L + WPT  C   P+ +G     W  E+   
Sbjct: 66  PNYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSC---PSNDGM--KFWTHEW--- 125

Query: 139 LRPEFKCIFNKDMKTIAKRHGTCASHVMKSEFEYFDQSLNLFLESNVNKALKNANIVASD 198
                            ++HGTCA   +  + +YF+  L L  ++N+  AL NA I   D
Sbjct: 126 -----------------EKHGTCAESEL-DQHDYFEAGLKLKQKANLLHALTNAGIKPDD 185

Query: 199 TVTYSKDEVIHVIEEAVLRGKPELICDDD---DEIVKELWLCYD 238
              Y   ++ + I++ V+   P + C+ D   +  + +++LC D
Sbjct: 186 KF-YEMKDIENTIKQ-VVGFAPGIECNKDSSHNSQLYQIYLCVD 196

BLAST of Lag0019782 vs. ExPASy Swiss-Prot
Match: Q7M438 (Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3)

HSP 1 Score: 82.0 bits (201), Expect = 1.1e-14
Identity = 67/259 (25.87%), Postives = 105/259 (40.54%), Query Frame = 0

Query: 4   LSLKAAAAAVLVAVAFVAQLQAARFGAEENEFDYFVLSLQWSGTSCRSNSVCCPESGCCA 63
           + L AA  +VL+  +           ++  +FD+++   QW  + C        +S  C 
Sbjct: 1   MRLIAALLSVLLIASTAQSTVTIYESSKPGDFDFYLFVQQWIYSYC--------DSQTCI 60

Query: 64  GADTPTQFTIHGLWPVKEWGKNPECCTDAVYNETEINPLKEELDKYWPTYQCGSQPTCNG 123
                  FTIHGLWP    G  P  C+   +N   I  L+++L+  W        P+  G
Sbjct: 61  QNKEREAFTIHGLWPENSDGSYPSFCSGPSFNVNAIQDLEDQLNFDW--------PSLTG 120

Query: 124 RTGSLWAEEYGKPLRPEFKCIFNKDMKTIAKRHGTCA-SHVMKSEFEYFDQSLNLFLESN 183
                W  E+ K                    HGTC+ +  +    +YF   + L+ E N
Sbjct: 121 PNTDFWTHEFSK--------------------HGTCSITGPITDIHDYFATGIKLYTEFN 180

Query: 184 VNKALKNANIVASDTVTYSKDEVIHVIEEAVLRGKPELICDDDDEIVKELWLCYDKR-LV 243
           +  AL++ NI  SD+ TY   ++ + I      GKP + C      +  + +C DK  L 
Sbjct: 181 ITAALESENIYPSDSNTYKPVDITNAI-TTHFGGKPGIQCSSGQ--LSTVAVCIDKNSLS 220

Query: 244 PRDCDPPTIESCSDSIKLP 261
             DC      SCS S+K P
Sbjct: 241 IMDCPDLQGWSCSGSVKFP 220

BLAST of Lag0019782 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 4.1e-12
Identity = 56/209 (26.79%), Postives = 96/209 (45.93%), Query Frame = 0

Query: 34  EFDYFVLSLQWSGTSCRSNSVCC-PESGCCAGADTPTQFTIHGLWPVKEWGKNPE-CCTD 93
           +FD+F    QW  + C +   CC P +G          F+IHGLWP  + GK P+ C  +
Sbjct: 27  DFDFFYFVQQWPASYCDTRRSCCYPTTG-----KPDEDFSIHGLWPNYKDGKWPQNCDRE 86

Query: 94  AVYNETEINPLKEELDKYWPTYQCGSQPTCNGRTGSLWAEEYGKPLRPEFKCIFNKDMKT 153
           +  +E+E + L   ++K WP+  C   P+ +G     W+ E+ K                
Sbjct: 87  SSLDESEFSDLISTMEKNWPSLAC---PSSDGL--KFWSHEWLK---------------- 146

Query: 154 IAKRHGTCASHVMKSEFEYFDQSLNLFLESNVNKALKNANIVASDTVTYSKDEVIHVIEE 213
               HGTC++    ++  YF  +L+   +SN+ + L NA I   +   Y  + +   IE+
Sbjct: 147 ----HGTCSA---LNQHAYFQTALDFKTKSNLLQNLNNAGIKPRNGDYYGVESIKKAIEK 201

Query: 214 AVLRGKPELICDDDDE---IVKELWLCYD 238
            V    P + C+ D +    + +++LC D
Sbjct: 207 GV-GHTPFIECNVDSQGNHQLYQVYLCVD 201

BLAST of Lag0019782 vs. ExPASy Swiss-Prot
Match: P04007 (Ribonuclease S-2 OS=Nicotiana alata OX=4087 GN=S-2 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 7.7e-11
Identity = 63/224 (28.12%), Postives = 93/224 (41.52%), Query Frame = 0

Query: 35  FDYFVLSLQWSGTSCRSNSVCCPESGCCAGADTPTQFTIHGLWPVKEWGKNPECCTDAVY 94
           F+Y  L L W  T CR     C          TPT FTIHGLWP         C     Y
Sbjct: 24  FEYMQLVLTWPITFCRIKH--CER--------TPTNFTIHGLWPDNHTTMLNYCDRSKPY 83

Query: 95  NETEINPLKEELDKYWPTYQCGSQPTCNGRTGSLWAEEYGKPLRPEFKCIFNKDMKTIAK 154
           N       K +LD+ WP        + + +  + W +EY K                   
Sbjct: 84  NMFTDGKKKNDLDERWPDLTKTKFDSLDKQ--AFWKDEYVK------------------- 143

Query: 155 RHGTCASHVMKSEFEYFDQSLNLFLESNVNKALKNANIVASDTVTYSKDEVIHVIEEAVL 214
            HGTC S     E +YFD ++ L  + ++  +L+N  I  S   +Y+   + + I +A+ 
Sbjct: 144 -HGTCCSDKFDRE-QYFDLAMTLRDKFDLLSSLRNHGI--SRGFSYTVQNLNNTI-KAIT 203

Query: 215 RGKPELICDDDDEIVKELWLCYDKRLVPRDCDPPTIESCSDSIK 259
            G P L C    E+ KE+ +C+D+  V    D P  ++C  + K
Sbjct: 204 GGFPNLTCSRLREL-KEIGICFDE-TVKNVIDCPNPKTCKPTNK 209

BLAST of Lag0019782 vs. ExPASy TrEMBL
Match: A0A6J1EA39 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111430737 PE=3 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 6.7e-58
Identity = 125/286 (43.71%), Postives = 168/286 (58.74%), Query Frame = 0

Query: 1   MASLSLKA-----AAAAVLVAVAFVAQLQAAR--FGAEENEFDYFVLSLQWSGTSCRSNS 60
           MAS S+K+     A A  L+ +  VA + AA    G E+ EFDYFVL+LQW  TSC++  
Sbjct: 1   MASFSIKSNPMVVAPAVFLLVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTG 60

Query: 61  VCCPESGCCAGADTPTQFTIHGLWPVKEWGKNPECCTDAVYNETEINPLKEELDKYWPTY 120
            CCP +GCC GAD+PT+FTIHGLWP       P CCTDAV+++ EI  LKE+++KYWPTY
Sbjct: 61  KCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFSQNEITLLKEDIEKYWPTY 120

Query: 121 QCGSQPTCNGRTGSLWAEEYGKPLRPEFKCIFNKDMKTIAKRHGTCASHVMKSEFEYFDQ 180
           +CG+   C+G  GS WA EY                    ++HGTCA+ V+  EF+YF  
Sbjct: 121 RCGTTSNCHGTKGSFWAHEY--------------------EKHGTCAAPVIVGEFDYFLT 180

Query: 181 SLNLFLESNVNKALKNANIVASDTVTYSKDEVIHVIEEAVLRGKPELICDDDDEIVKELW 240
           +L +F + NV KAL +A  VAS+T  Y  ++V+  ++ A     P++ C      VKELW
Sbjct: 181 TLTIFSKYNVTKALSDAGFVASNTEKYPLEDVVDAVKNAT-NSTPKISCAKKG-AVKELW 240

Query: 241 LCYDKRLVPRDCDPPTIESCSDSIKLPLKPPPSETCCGANGAISWM 280
           +C+DK+  PRDCD     SC + IKLP   P         GAI W+
Sbjct: 241 ICFDKKFEPRDCD--LANSCPEFIKLPTYEPQDV----VEGAIPWI 258

BLAST of Lag0019782 vs. ExPASy TrEMBL
Match: A0A6J1CHM7 (Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111011505 PE=3 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 1.5e-57
Identity = 118/256 (46.09%), Postives = 160/256 (62.50%), Query Frame = 0

Query: 5   SLKAAAAAVLVAVAFVAQLQAARFGAEENEFDYFVLSLQWSGTSCRSNSVCCPESGCCAG 64
           S   AA A  V V  V QLQ A F  ++ EFDYFVL+LQW G++C++ S CC ++GCC G
Sbjct: 8   SNSVAAVAAAVFVVAVVQLQTAAFAKDQREFDYFVLALQWPGSACKNISYCCQDNGCCRG 67

Query: 65  ADTPTQFTIHGLWPVKEWGKNPECCTDAVYNETEINPLKEELDKYWPTYQCGSQPTCNGR 124
           + TPTQFTIHGLWP  E    P CCTD  +NE EI  L++ L + WP+Y+CGS  +CN +
Sbjct: 68  SQTPTQFTIHGLWPQYEVKGWPSCCTDESFNENEIFVLRKILPEIWPSYRCGSVTSCNNK 127

Query: 125 TGSLWAEEYGKPLRPEFKCIFNKDMKTIAKRHGTCASHVMKSEFEYFDQSLNLFLESNVN 184
            GS WAEEY                    ++HGTCA+ V++ ++ YF  ++ LF   NV 
Sbjct: 128 KGSFWAEEY--------------------EKHGTCAAPVIEGQYNYFSTAIGLFNTHNVT 187

Query: 185 KALKNANIVASDTVTYSKDEVIHVIEEAVLRGKPELICDDDDEIVKELWLCYDKRLVPRD 244
           KAL+NA IVASDTV Y  ++V+  +E A   G P+++C     I+KEL+LC+DK+  PR+
Sbjct: 188 KALENAGIVASDTVKYPLNDVMAAVEFA-FGGTPKIMCGKKG-IIKELYLCFDKKFKPRN 239

Query: 245 CDPPTIESCSDSIKLP 261
           CD    +SC   +KLP
Sbjct: 248 CD--ETKSCPKKVKLP 239

BLAST of Lag0019782 vs. ExPASy TrEMBL
Match: A0A6J1CGP7 (Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111011504 PE=3 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 3.3e-57
Identity = 128/287 (44.60%), Postives = 165/287 (57.49%), Query Frame = 0

Query: 1   MASLSLKA-AAAAVLVAVAFVAQLQAARFGA-------EENEFDYFVLSLQWSGTSCRSN 60
           MAS S+K    AA  V +  V QLQAA F A       E+ EFDYFVL+LQW  TSC + 
Sbjct: 1   MASSSIKGNPTAAATVFLLVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTNP 60

Query: 61  SVCCPESGCCAGADTPTQFTIHGLWPVKEWGKNPECCTDAVYNETEINPLKEELDKYWPT 120
             CCP +GCC GA++PT+FTIHGLWP  E    P CC+DA ++E EI  L +++ KYWPT
Sbjct: 61  GKCCPSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYWPT 120

Query: 121 YQCGSQPTCNGRTGSLWAEEYGKPLRPEFKCIFNKDMKTIAKRHGTCASHVMKSEFEYFD 180
           Y+CG+  TC+   GS WA EY                    ++HGTCA+ V+  EF+YF 
Sbjct: 121 YRCGTTSTCHRTKGSFWAHEY--------------------EKHGTCAAPVIVGEFDYFL 180

Query: 181 QSLNLFLESNVNKALKNANIVASDTVTYSKDEVIHVIEEAVLRGKPELICDDDDEIVKEL 240
            +L +F + NV K L +A  VASDT  Y+  +V+  +++A     P++ C      VKEL
Sbjct: 181 TTLTIFSKYNVTKVLNDAGFVASDTEKYALGDVVAAVQDA-FNATPKISCAKKG-AVKEL 240

Query: 241 WLCYDKRLVPRDCDPPTIESCSDSIKLPLKPPPSETCCGANGAISWM 280
           WLC+DK   PRDCD     SC D IKLP   P  +      GAI WM
Sbjct: 241 WLCFDKTFQPRDCD--LANSCPDFIKLPTYEP--QEANKVEGAIPWM 261

BLAST of Lag0019782 vs. ExPASy TrEMBL
Match: A0A0A0L0P9 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_4G109540 PE=3 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 4.8e-56
Identity = 123/287 (42.86%), Postives = 163/287 (56.79%), Query Frame = 0

Query: 1   MASLSLKAAAAAVLVAVAFVAQLQAARFG--------AEENEFDYFVLSLQWSGTSCRSN 60
           MASLS+KA A   +V V  + QL    FG        +E+ EFDYFVL+LQW G+SC++ 
Sbjct: 1   MASLSIKAKAMVFVVVV--LLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNP 60

Query: 61  SVCCPESGCCAGADTPTQFTIHGLWPVKEWGKNPECCTDAVYNETEINPLKEELDKYWPT 120
             CCP + CC GA++PT+FTIHGLWP       P CCTDA +NE EIN L E++ KYWPT
Sbjct: 61  GKCCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPT 120

Query: 121 YQCGSQPTCNGRTGSLWAEEYGKPLRPEFKCIFNKDMKTIAKRHGTCASHVMKSEFEYFD 180
           Y+CG+  TC+   GS WA EY                    ++HGTCA+ V+  E++YF 
Sbjct: 121 YRCGTTSTCHQTKGSFWAHEY--------------------EKHGTCAAPVIVGEYDYFL 180

Query: 181 QSLNLFLESNVNKALKNANIVASDTVTYSKDEVIHVIEEAVLRGKPELICDDDDEIVKEL 240
            ++ +F + NV K L +A  VAS+T  Y  ++V+  I+       P++ C      VKEL
Sbjct: 181 TTITIFSKYNVTKVLSDAGFVASNTEKYPIEDVVAAIKNEFNNATPKISCAKKG-AVKEL 240

Query: 241 WLCYDKRLVPRDCDPPTIESCSDSIKLPLKPPPSETCCGANGAISWM 280
           WLC+DK   PRDC+ P   SC D IK P   P         GAI W+
Sbjct: 241 WLCFDKSFEPRDCNLP--NSCPDFIKFPTYEPQEPN--KVEGAIPWI 260

BLAST of Lag0019782 vs. ExPASy TrEMBL
Match: A0A6J1J670 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111482949 PE=3 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 1.1e-55
Identity = 123/288 (42.71%), Postives = 165/288 (57.29%), Query Frame = 0

Query: 1   MASLSLK------AAAAAVLVAVAFVAQLQAARF---GAEENEFDYFVLSLQWSGTSCRS 60
           MAS  +K      A A  +L+A+  VA + AA     G  + EFDYFVL+LQW  TSC++
Sbjct: 1   MASFYIKSNPMVVAPAVFLLLALQLVAVVLAAETPSGGGGQKEFDYFVLALQWPATSCKN 60

Query: 61  NSVCCPESGCCAGADTPTQFTIHGLWPVKEWGKNPECCTDAVYNETEINPLKEELDKYWP 120
              CCP +GCC GAD+PT+FTIHGLWP       P CCTDAV+N+ EI+ L E+++KYWP
Sbjct: 61  TGKCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLSEDIEKYWP 120

Query: 121 TYQCGSQPTCNGRTGSLWAEEYGKPLRPEFKCIFNKDMKTIAKRHGTCASHVMKSEFEYF 180
           TY+CG+   C+G  GS WA EY                    ++HGTCA+ V+  E++YF
Sbjct: 121 TYRCGTTSNCHGTKGSFWAHEY--------------------EKHGTCAAPVIVGEYDYF 180

Query: 181 DQSLNLFLESNVNKALKNANIVASDTVTYSKDEVIHVIEEAVLRGKPELICDDDDEIVKE 240
             +L +F + NV KAL +A  VAS+T  Y   +V+  ++ A     P++ C      VKE
Sbjct: 181 LTTLTIFSKYNVTKALSDAGFVASNTEKYPLGDVVDAVKNAT-NSTPKISCAKKG-AVKE 240

Query: 241 LWLCYDKRLVPRDCDPPTIESCSDSIKLPLKPPPSETCCGANGAISWM 280
           LW+C+DK   PRDCD     SC + IKLP   P         GAI W+
Sbjct: 241 LWICFDKNFEPRDCD--LANSCPEFIKLPTYEPQGV----VEGAIPWI 260

BLAST of Lag0019782 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 179.9 bits (455), Expect = 2.9e-45
Identity = 100/264 (37.88%), Postives = 137/264 (51.89%), Query Frame = 0

Query: 8   AAAAAVLVAVAFVAQLQAA---RFGAEENEFDYFVLSLQWSGTSCRSNSVCCPESGCCAG 67
           A+   +L+ VA +A   A         + EFDYF LSLQW GT CR    CC ++ CC G
Sbjct: 2   ASRLCLLLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRG 61

Query: 68  ADTPTQFTIHGLWPVKEWGKNPECCTDAVYNETEINPLKEELDKYWPTYQCGSQPTCNGR 127
           +D PTQFTIHGLWP    G  P CC  + + E EI+ L + L+KYWP+  CGS  +CNG 
Sbjct: 62  SDAPTQFTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGG 121

Query: 128 TGSLWAEEYGKPLRPEFKCIFNKDMKTIAKRHGTCASHVMKSEFEYFDQSLNLFLESNVN 187
            GS W  E+                    ++HGTC+S V   E+ YF  +LNL+L+ NV 
Sbjct: 122 KGSFWGHEW--------------------EKHGTCSSPVFHDEYNYFLTTLNLYLKHNVT 181

Query: 188 KALKNANIVASDTVTYSKDEVIHVIEEAVLRGKPELICDDDDEIVKELWLCYDKRLVPRD 247
             L  A  VAS++  Y    ++  I+ A     PE++C  D   + E+ +C+ K   PRD
Sbjct: 182 DVLYQAGYVASNSEKYPLGGIVTAIQNA-FHITPEVVCKRD--AIDEIRICFYKDFKPRD 241

Query: 248 C----DPPTIESCSDSIKLPLKPP 265
           C    D  + +SC   + LP   P
Sbjct: 242 CVGSQDLTSRKSCPKYVSLPEYTP 242

BLAST of Lag0019782 vs. TAIR 10
Match: AT2G39780.2 (ribonuclease 2 )

HSP 1 Score: 134.4 bits (337), Expect = 1.4e-31
Identity = 85/264 (32.20%), Postives = 114/264 (43.18%), Query Frame = 0

Query: 8   AAAAAVLVAVAFVAQLQAA---RFGAEENEFDYFVLSLQWSGTSCRSNSVCCPESGCCAG 67
           A+   +L+ VA +A   A         + EFDYF LSLQW GT CR    CC ++ CC G
Sbjct: 2   ASRLCLLLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRG 61

Query: 68  ADTPTQFTIHGLWPVKEWGKNPECCTDAVYNETEINPLKEELDKYWPTYQCGSQPTCNGR 127
           +D PTQFTIHGLWP    G  P CC  + + E EI+ L + L+KYWP+  CGS  +CNG 
Sbjct: 62  SDAPTQFTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGG 121

Query: 128 TGSLWAEEYGKPLRPEFKCIFNKDMKTIAKRHGTCASHVMKSEFEYFDQSLNLFLESNVN 187
            GS W  E                                                    
Sbjct: 122 KGSFWGHE---------------------------------------------------- 181

Query: 188 KALKNANIVASDTVTYSKDEVIHVIEEAVLRGKPELICDDDDEIVKELWLCYDKRLVPRD 247
             L  A  VAS++  Y    ++  I+ A     PE++C  D   + E+ +C+ K   PRD
Sbjct: 182 DVLYQAGYVASNSEKYPLGGIVTAIQNA-FHITPEVVCKRD--AIDEIRICFYKDFKPRD 210

Query: 248 C----DPPTIESCSDSIKLPLKPP 265
           C    D  + +SC   + LP   P
Sbjct: 242 CVGSQDLTSRKSCPKYVSLPEYTP 210

BLAST of Lag0019782 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 85.5 bits (210), Expect = 7.4e-17
Identity = 63/224 (28.12%), Postives = 104/224 (46.43%), Query Frame = 0

Query: 19  FVAQLQAARFGAEENEFDYFVLSLQWSGTSCRSNSVCC-PESGCCAGADTPTQFTIHGLW 78
           F+  LQ     +   +FD+F   LQW G  C S   CC P++G  A       F IHGLW
Sbjct: 6   FILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPA-----ADFGIHGLW 65

Query: 79  PVKEWGKNPECCT-DAVYNETEINPLKEELDKYWPTYQCGSQPTCNGRTGSLWAEEYGKP 138
           P  + G  P+ C  D+ +++  ++ L  +L + WPT  C   P+ +G     W  E+   
Sbjct: 66  PNYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSC---PSNDGM--KFWTHEW--- 125

Query: 139 LRPEFKCIFNKDMKTIAKRHGTCASHVMKSEFEYFDQSLNLFLESNVNKALKNANIVASD 198
                            ++HGTCA   +  + +YF+  L L  ++N+  AL NA I   D
Sbjct: 126 -----------------EKHGTCAESEL-DQHDYFEAGLKLKQKANLLHALTNAGIKPDD 185

Query: 199 TVTYSKDEVIHVIEEAVLRGKPELICDDD---DEIVKELWLCYD 238
              Y   ++ + I++ V+   P + C+ D   +  + +++LC D
Sbjct: 186 KF-YEMKDIENTIKQ-VVGFAPGIECNKDSSHNSQLYQIYLCVD 196

BLAST of Lag0019782 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 69.7 bits (169), Expect = 4.2e-12
Identity = 63/258 (24.42%), Postives = 107/258 (41.47%), Query Frame = 0

Query: 13  VLVAVAFVAQLQAARFGAEENEFDYFVLSLQWSGTSCRSNSVCCPESGCCAGADTPTQFT 72
           +++    + Q         E +F++F     W G  C S   CCP +      +T + F 
Sbjct: 4   IIIVSLLILQSLVVSSSQTEPDFNFFYWVNYWPGAICDSQKGCCPPT----KGNTASDFI 63

Query: 73  IHGLWPVKEWGKNPECCTDA-VYNETEINPLKEELDKYWPTYQCGSQPTCNGRTGSLWAE 132
           IHGLWP    G  P  C    +++ ++I+ L  +++K W  +   +   C     +LW  
Sbjct: 64  IHGLWPQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWA---CPSNETNLWEH 123

Query: 133 EYGKPLRPEFKCIFNKDMKTIAKRHGTCASHVMKSEFEYFDQSLNLFLESNVNKALKNAN 192
           E+ K                    HGTC   +   +  YF  +L    + ++   L    
Sbjct: 124 EWNK--------------------HGTCVQSIF-DQHSYFRTNLKFKHKVHLLNILIQKG 183

Query: 193 IVASDTVTYSKDEVIHVIEEAVLRGKPELICDDDDEIVKEL---WLC---YDKRLVPRDC 252
           I  +D   YS DE+ + I+ A+    P + C++D +  K+L   ++C   Y K  V  +C
Sbjct: 184 IKPNDGF-YSLDEIKNAIKCAI-GFAPGIECNEDVKGNKQLFQIYICLDNYAKEFV--EC 229

Query: 253 DPPTIESCSDSIKLPLKP 264
                +SC+  IK P  P
Sbjct: 244 PYVPDKSCASKIKFPSLP 229

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022922895.11.4e-5743.71ribonuclease 2-like [Cucurbita moschata][more]
XP_022140986.13.1e-5746.09ribonuclease 2-like [Momordica charantia][more]
XP_022140985.16.8e-5744.60ribonuclease 2-like [Momordica charantia][more]
XP_004137162.19.9e-5642.86ribonuclease 2 [Cucumis sativus][more]
XP_038876700.11.3e-5543.55ribonuclease 2-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
P428144.0e-4437.88Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1[more]
P428151.0e-1528.13Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1[more]
Q7M4381.1e-1425.87Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3[more]
P801964.1e-1226.79Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2[more]
P040077.7e-1128.13Ribonuclease S-2 OS=Nicotiana alata OX=4087 GN=S-2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EA396.7e-5843.71Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111430737 PE=3 SV=1[more]
A0A6J1CHM71.5e-5746.09Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111011505 PE=3 SV=1[more]
A0A6J1CGP73.3e-5744.60Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111011504 PE=3 SV=1[more]
A0A0A0L0P94.8e-5642.86Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_4G109540 PE=3 SV=1[more]
A0A6J1J6701.1e-5542.71Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111482949 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G39780.12.9e-4537.88ribonuclease 2 [more]
AT2G39780.21.4e-3132.20ribonuclease 2 [more]
AT1G26820.17.4e-1728.13ribonuclease 3 [more]
AT1G14210.14.2e-1224.42Ribonuclease T2 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036430Ribonuclease T2-like superfamilyGENE3D3.90.730.10coord: 31..263
e-value: 4.7E-59
score: 201.9
IPR036430Ribonuclease T2-like superfamilySUPERFAMILY55895Ribonuclease Rh-likecoord: 33..261
IPR001568Ribonuclease T2-likePFAMPF00445Ribonuclease_T2coord: 35..135
e-value: 1.6E-16
score: 61.0
coord: 154..245
e-value: 6.8E-16
score: 58.9
IPR001568Ribonuclease T2-likePANTHERPTHR11240RIBONUCLEASE T2coord: 18..249
NoneNo IPR availablePANTHERPTHR11240:SF51RIBONUCLEASE 2coord: 18..249
IPR018188Ribonuclease T2, His active site 1PROSITEPS00530RNASE_T2_1coord: 71..78
IPR033697Ribonuclease T2, eukaryoticCDDcd01061RNase_T2_eukcoord: 35..256
e-value: 1.3889E-47
score: 155.568

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0019782.1Lag0019782.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090304 nucleic acid metabolic process
molecular_function GO:0033897 ribonuclease T2 activity
molecular_function GO:0003723 RNA binding