Lag0019780 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0019780
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Ribonuclease T(2)
Location	chr5: 45425638 .. 45428931 (+)
RNA-Seq Expression	Lag0019780
Synteny	Lag0019780

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCTCTGTCGTTCAAGGCGGCGGCGGCGGCGGTGCTTGTGGCCGTTGCTGTTGTTGCTCAGCTGCAGGCGGCGAGGTTTGCGGCGGATCAGAAGGAATTTGACTACTTTGTGTTGTCACTGCAATGGCCAGGCAATTCCTGCAGAGACATAAGCATGTGTTGCCAAGAAAACGCCTGCTGCGCTGGGTGAGACATCAGTTGTTTATGTTTTTTGTAATGACTTTTATTGTTGCTTTTTGTTTTGTTTTTGTTTTTTTTTTTTTTTTCATGTCGTGTTGTACAATAATAATGTGCTAATTAAGATTTTTGTGTGTTTATTCTTTCTCTAGAGCTGACACTTCCAAAGAATTTACAATCCGTAAGTGTTAAAAAAGCTGACCTTTTTTGGATTTTGCTAATTTTGATTTTTAATTTGGATGTGTTGTTGAATAATTAATTAAATTGGATTTATATGATCATCTAATTAAGAGTGTGTTTTCTGTTTTTCATTGGTGCAGATGGGCTGTGGCCTGAGTATGAATGGAATGGATGGCCTCAATGTTGTACTGATGCCATATTTAATGAAAATGAGGTCTCTCTCAAATTTAATGGTGCAAGGACTTGAAAACTATGCTCGGTTTGGACCGACTTTTCAACCGGTTAAAAAATATTTTTTGAACACTTGAAAAATCATTCCAAAACCTATTTTGACACAAACATATGAAAGTTAATCAACTAAACTTTTAGTTTGTAATTTAACCTTAATATTATATACACATATTAATGGTCTTTTGTTACTATATATAAATATTGAAATGAATATTTTTCAAAAAAAAAAAAAAATATTGAAATGAATATTGGTTTTGGGCCCAGTTTATAAAGAAAAAAGTCTTTACAATGATTTATCCTGTCAACATAAATATAAACATATATTGATGTTTTTATAGTTGTCGATATATGTATTTCTTAAACAATAATTTATTTCTAGGTCAAAATAAAATTTTTATTGACGTGTTTTTCTTACAAGCGTCAACTCAACAAAAAAAAAGTCAAATGTCAATAAACGCCAAAATTGTAGGTACTGGTTCTATACCAATTATTTTACAGTACAATGAAAATGTTGATATCGTCAGATTCATAGACATGTAGAAAAGTCGGAAAAAATGTCGTTGGAAATTTAATACTCTATCATTACCAACTTCTAATTATACATACATATATATATATATTCTCTTTTCTAACGTAATTTCTCTCTCTTCAATTCTAGTTAATATATTTACTTTTTCTCTCTAATATCTTTATGGCAGGGACTTACTGTACACAAAATTGAAATGTTAAAAAGGTTTATTAACTAATTTTTTTTTAAAAGTATATGGACTAAAAATCCATAATTTTGAAAGTTTATGAACCTAGCTAAATTTAAAATTTTAGGAAGCAAACATGTAATCAATTAACCTAAAAAGTTAAGTGTACTCTAAACTATATTAAAAAGATTAAATATACTTGTAGATTAATCCCTTGATGGACGCTCTTGACAAGTATTGGCCAACCTATCGATGTGGTTCTGAACCAACCTGCAGTGATAGAACAGGAAACTTTTGGGCTCAACAGGTTCAAATTATAAATCTCTCTTTCTCCTTTAATTTTCTTCCTTAATTACCTCATTATCAAAATTTGAGACTAATCCTAATTTCTCTCATCTTTTGTCTCTCTCTTGTTGCCATTGCTGCAGTATGGTAAGCCACTTCGACTCGTACGTTTCTTTGTTTTGTTACTTACTCTCTAGAAATATTCTCAAAACCTCAAAAAGTAACTTTTAAAAACTTGTTTTTGTTTCTGGGATTTGACTAAAATAGTTCAAATTGATTCCTAAAACTCAAAAACCAAAGACCAAATGTTCATGAAACGGATCTGATCTTGACAGAGAAGCATGGAACTTGCGCATCTCCTGTAATCAACAGTCAACTTGAATACTTCAACACAACCCTCGAGTTGTTCTCAAAGTACAATCTCAATGTAAGCTTTTATAAACTTTCTTTTGACAAATAAATTTTATGTCAGTTGAGCTAGCTTTTGTTTGGCTAAGTTTTTATAAACTTGGTTATCGATTTTCAACCGTTAAATTAAAATTAAACTTTGCACATAATATATATTTATAAAAAAAAAAAATTTGTTTTTTGTTTTTGTTACTCAGAAAGCTTTGCAGAATGCTGAAATCGTACCTTCGGATACTGCAACGTATCCCAAAAAAGATGTGATCGATGTAGTTACGACTGTTTTACAAGGAGGAAAACCACAGCTTAGATGTGCTGAAGAGGGGATTGTCAAGGAAGTTTGGCTATGCTATGATAAACTATTTTTGGTATTACTTTGTGTTTATAAAATAAATCAATATTCCTAACTGTGACAGATCCATTGATGAAGAAAAGCTAAATCTTTACTCTTTGCAGCCTCGTGATTGCAATCCTATTAAAGAAACATGCCCCGACTCTGTTAAGTTCCCGATAGCACCGACAAGGGGTAAGTTTCTTTCTATCTAAGGATCTAAATATTTGTCGGTGGGCGTCGGTTTTGAACTTAAAAAACTGACTTCTCTTTAATCAAAGATCATCGAACCACAATGACGGTTGGTTAGTTTTTAACTCAAACCAAAAAGGGAGAAGAGAAAAGAAAAATATATAATTTACTTTTAAAACCACTTGAGAAAATCAACAGTATCGTTGGTTTTCTCAAGTGGTTTTGCACATTGCTTAAATTTTCTTGTTGGAGGTCGGTCGATCGGTTACTCGCTTTCAAATCAGATTGACCGATTGATAGTTGATTTTTTATATGCCACAAACCAACTTCAGTCGAACTCATTGATCAAATTGATTGACCTATAACGTCAATTCAGTCGATTTTCGACGGTCAGGCCAGTTTTTTTTATTTCTCATATCCACCCCTAAGCAAAAGTGTCTTAATTAGGGTTTCTTTATTACCACACCTACCTCATTAGAAAATAACTTTTTTACGATACCTTTACAAAATCTTAGACAATAAAGTTTTCTTTGTTAAATAAATAACCGCTACATTCAAAAGCTTACCTGAAGTTTCGAAAATTAATAAAAAAAAACCATATACGTAGACTAGAATAATCAAAGAGAGAAATGAAGTTGAAGAGATTGAAACACACCAGATACATCTCGTGTTTCGATACTATATCGAATCGTCATTCAACTCAAAAGTTTTAGACTAATAATTAATCAACATTTATGTTTTGTGTATTATTGCAGGGCCTTGCTTGTCAAATGGAGCACTTCCATGGATGTAG

mRNA sequence

ATGGCTTCTCTGTCGTTCAAGGCGGCGGCGGCGGCGGTGCTTGTGGCCGTTGCTGTTGTTGCTCAGCTGCAGGCGGCGAGGTTTGCGGCGGATCAGAAGGAATTTGACTACTTTGTGTTGTCACTGCAATGGCCAGGCAATTCCTGCAGAGACATAAGCATGTGTTGCCAAGAAAACGCCTGCTGCGCTGGAGCTGACACTTCCAAAGAATTTACAATCCATGGGCTGTGGCCTGAGTATGAATGGAATGGATGGCCTCAATGTTGTACTGATGCCATATTTAATGAAAATGAGATTAATCCCTTGATGGACGCTCTTGACAAGTATTGGCCAACCTATCGATGTGGTTCTGAACCAACCTGCAGTGATAGAACAGGAAACTTTTGGGCTCAACAGTATGAGAAGCATGGAACTTGCGCATCTCCTGTAATCAACAGTCAACTTGAATACTTCAACACAACCCTCGAGTTGTTCTCAAAGTACAATCTCAATAAAGCTTTGCAGAATGCTGAAATCGTACCTTCGGATACTGCAACGTATCCCAAAAAAGATGTGATCGATGTAGTTACGACTGTTTTACAAGGAGGAAAACCACAGCTTAGATGTGCTGAAGAGGGGATTGTCAAGGAAGTTTGGCTATGCTATGATAAACTATTTTTGCCTCGTGATTGCAATCCTATTAAAGAAACATGCCCCGACTCTGTTAAGTTCCCGATAGCACCGACAAGGGGGCCTTGCTTGTCAAATGGAGCACTTCCATGGATGTAG

Coding sequence (CDS)

ATGGCTTCTCTGTCGTTCAAGGCGGCGGCGGCGGCGGTGCTTGTGGCCGTTGCTGTTGTTGCTCAGCTGCAGGCGGCGAGGTTTGCGGCGGATCAGAAGGAATTTGACTACTTTGTGTTGTCACTGCAATGGCCAGGCAATTCCTGCAGAGACATAAGCATGTGTTGCCAAGAAAACGCCTGCTGCGCTGGAGCTGACACTTCCAAAGAATTTACAATCCATGGGCTGTGGCCTGAGTATGAATGGAATGGATGGCCTCAATGTTGTACTGATGCCATATTTAATGAAAATGAGATTAATCCCTTGATGGACGCTCTTGACAAGTATTGGCCAACCTATCGATGTGGTTCTGAACCAACCTGCAGTGATAGAACAGGAAACTTTTGGGCTCAACAGTATGAGAAGCATGGAACTTGCGCATCTCCTGTAATCAACAGTCAACTTGAATACTTCAACACAACCCTCGAGTTGTTCTCAAAGTACAATCTCAATAAAGCTTTGCAGAATGCTGAAATCGTACCTTCGGATACTGCAACGTATCCCAAAAAAGATGTGATCGATGTAGTTACGACTGTTTTACAAGGAGGAAAACCACAGCTTAGATGTGCTGAAGAGGGGATTGTCAAGGAAGTTTGGCTATGCTATGATAAACTATTTTTGCCTCGTGATTGCAATCCTATTAAAGAAACATGCCCCGACTCTGTTAAGTTCCCGATAGCACCGACAAGGGGGCCTTGCTTGTCAAATGGAGCACTTCCATGGATGTAG

Protein sequence

MASLSFKAAAAAVLVAVAVVAQLQAARFAADQKEFDYFVLSLQWPGNSCRDISMCCQENACCAGADTSKEFTIHGLWPEYEWNGWPQCCTDAIFNENEINPLMDALDKYWPTYRCGSEPTCSDRTGNFWAQQYEKHGTCASPVINSQLEYFNTTLELFSKYNLNKALQNAEIVPSDTATYPKKDVIDVVTTVLQGGKPQLRCAEEGIVKEVWLCYDKLFLPRDCNPIKETCPDSVKFPIAPTRGPCLSNGALPWM

Homology

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BLAST of Lag0019780 vs. NCBI nr
Match: XP_004137162.1 (ribonuclease 2 [Cucumis sativus])

HSP 1 Score: 298.1 bits (762), Expect = 7.1e-77
Identity = 137/261 (52.49%), Postives = 177/261 (67.82%), Query Frame = 0


Query: 1   MASLSFKAAAAAVLVAV-----AVVAQLQA-ARFAADQKEFDYFVLSLQWPGNSCRDISM 60
           MASLS KA A   +V V      VV    A A  +++Q+EFDYFVL+LQWPG+SC++   
Sbjct: 1   MASLSIKAKAMVFVVVVLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGK 60

Query: 61  CCQENACCAGADTSKEFTIHGLWPEYEWNGWPQCCTDAIFNENEINPLMDALDKYWPTYR 120
           CC  NACC GA++  EFTIHGLWP+Y   GWP CCTDA FNENEIN L + + KYWPTYR
Sbjct: 61  CCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYR 120

Query: 121 CGSEPTCSDRTGNFWAQQYEKHGTCASPVINSQLEYFNTTLELFSKYNLNKALQNAEIVP 180
           CG+  TC    G+FWA +YEKHGTCA+PVI  + +YF TT+ +FSKYN+ K L +A  V 
Sbjct: 121 CGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVA 180

Query: 181 SDTATYPKKDVIDVVTTVLQGGKPQLRCAEEGIVKEVWLCYDKLFLPRDCNPIKETCPDS 240
           S+T  YP +DV+  +        P++ CA++G VKE+WLC+DK F PRDCN +  +CPD 
Sbjct: 181 SNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCN-LPNSCPDF 240

Query: 241 VKFPIAPTRGPCLSNGALPWM 256
           +KFP    + P    GA+PW+
Sbjct: 241 IKFPTYEPQEPNKVEGAIPWI 260

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BLAST of Lag0019780 vs. NCBI nr
Match: XP_022140986.1 (ribonuclease 2-like [Momordica charantia])

HSP 1 Score: 295.0 bits (754), Expect = 6.0e-76
Identity = 134/251 (53.39%), Postives = 174/251 (69.32%), Query Frame = 0


Query: 5   SFKAAAAAVLVAVAVVAQLQAARFAADQKEFDYFVLSLQWPGNSCRDISMCCQENACCAG 64
           S   AA A  V V  V QLQ A FA DQ+EFDYFVL+LQWPG++C++IS CCQ+N CC G
Sbjct: 8   SNSVAAVAAAVFVVAVVQLQTAAFAKDQREFDYFVLALQWPGSACKNISYCCQDNGCCRG 67

Query: 65  ADTSKEFTIHGLWPEYEWNGWPQCCTDAIFNENEINPLMDALDKYWPTYRCGSEPTCSDR 124
           + T  +FTIHGLWP+YE  GWP CCTD  FNENEI  L   L + WP+YRCGS  +C+++
Sbjct: 68  SQTPTQFTIHGLWPQYEVKGWPSCCTDESFNENEIFVLRKILPEIWPSYRCGSVTSCNNK 127

Query: 125 TGNFWAQQYEKHGTCASPVINSQLEYFNTTLELFSKYNLNKALQNAEIVPSDTATYPKKD 184
            G+FWA++YEKHGTCA+PVI  Q  YF+T + LF+ +N+ KAL+NA IV SDT  YP  D
Sbjct: 128 KGSFWAEEYEKHGTCAAPVIEGQYNYFSTAIGLFNTHNVTKALENAGIVASDTVKYPLND 187

Query: 185 VIDVVTTVLQGGKPQLRCAEEGIVKEVWLCYDKLFLPRDCNPIKETCPDSVKFPIAPTRG 244
           V+  V     GG P++ C ++GI+KE++LC+DK F PR+C+  K +CP  VK P      
Sbjct: 188 VMAAVEFAF-GGTPKIMCGKKGIIKELYLCFDKKFKPRNCDETK-SCPKKVKLPTIEQLE 247

Query: 245 PCLSNGALPWM 256
             +  GA+P M
Sbjct: 248 SYMGEGAIPRM 256

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BLAST of Lag0019780 vs. NCBI nr
Match: KAE8649307.1 (hypothetical protein Csa_015411 [Cucumis sativus])

HSP 1 Score: 287.7 bits (735), Expect = 9.6e-74
Identity = 132/244 (54.10%), Postives = 169/244 (69.26%), Query Frame = 0


Query: 1   MASLSFKAAAAAVLVAV-----AVVAQLQA-ARFAADQKEFDYFVLSLQWPGNSCRDISM 60
           MASLS KA A   +V V      VV    A A  +++Q+EFDYFVL+LQWPG+SC++   
Sbjct: 1   MASLSIKAKAMVFVVVVLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGK 60

Query: 61  CCQENACCAGADTSKEFTIHGLWPEYEWNGWPQCCTDAIFNENEINPLMDALDKYWPTYR 120
           CC  NACC GA++  EFTIHGLWP+Y   GWP CCTDA FNENEIN L + + KYWPTYR
Sbjct: 61  CCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYR 120

Query: 121 CGSEPTCSDRTGNFWAQQYEKHGTCASPVINSQLEYFNTTLELFSKYNLNKALQNAEIVP 180
           CG+  TC    G+FWA +YEKHGTCA+PVI  + +YF TT+ +FSKYN+ K L +A  V 
Sbjct: 121 CGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVA 180

Query: 181 SDTATYPKKDVIDVVTTVLQGGKPQLRCAEEGIVKEVWLCYDKLFLPRDCNPIKETCPDS 239
           S+T  YP +DV+  +        P++ CA++G VKE+WLC+DK F PRDCN +  +CPD 
Sbjct: 181 SNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCN-LPNSCPDF 240

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BLAST of Lag0019780 vs. NCBI nr
Match: XP_008462866.1 (PREDICTED: ribonuclease 2-like [Cucumis melo])

HSP 1 Score: 284.6 bits (727), Expect = 8.1e-73
Identity = 130/260 (50.00%), Postives = 167/260 (64.23%), Query Frame = 0


Query: 1   MASLSFKAAAAAVLVAV----AVVAQLQA-ARFAADQKEFDYFVLSLQWPGNSCRDISMC 60
           MAS S K      L  +     VV    A A   ++Q+EFDYFVL+LQWP  SC++   C
Sbjct: 1   MASFSIKGKPMVFLFLIVELLTVVFGFSAEAPSGSEQREFDYFVLALQWPATSCKNAGKC 60

Query: 61  CQENACCAGADTSKEFTIHGLWPEYEWNGWPQCCTDAIFNENEINPLMDALDKYWPTYRC 120
           C  NACC GAD+  EFTIHGLWP+Y   GWP CCTDA FNENEI  L + + KYWPTYRC
Sbjct: 61  CPSNACCRGADSPTEFTIHGLWPQYNEKGWPSCCTDASFNENEIGILAEDIQKYWPTYRC 120

Query: 121 GSEPTCSDRTGNFWAQQYEKHGTCASPVINSQLEYFNTTLELFSKYNLNKALQNAEIVPS 180
           G+  TC    G+FWA +YEKHGTCA+PV+  + +YF T L +FSKYN+ K L     V S
Sbjct: 121 GTSSTCHRTKGSFWAHEYEKHGTCAAPVLVGEYDYFLTALTIFSKYNVTKVLSEGGFVAS 180

Query: 181 DTATYPKKDVIDVVTTVLQGGKPQLRCAEEGIVKEVWLCYDKLFLPRDCNPIKETCPDSV 240
           +T  YP +DVI  +        P++ CA++G+VKE+WLC+DK F PRDCN ++ +CPD +
Sbjct: 181 NTEKYPIEDVIAAIKNKFYNATPKITCAKKGVVKELWLCFDKSFEPRDCN-LQHSCPDFI 240

Query: 241 KFPIAPTRGPCLSNGALPWM 256
           K P    + P    GA+PW+
Sbjct: 241 KLPTYEPQEPNKVEGAIPWI 259

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BLAST of Lag0019780 vs. NCBI nr
Match: XP_038876678.1 (ribonuclease 2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 284.3 bits (726), Expect = 1.1e-72
Identity = 135/242 (55.79%), Postives = 169/242 (69.83%), Query Frame = 0


Query: 1   MASLSFKAAAAAVLVAV-AVVAQLQAAR---FAADQKEFDYFVLSLQWPGNSCRDISMCC 60
           M S S KAAA  V V V  +V  LQ A       +Q+EFDYFVL+LQWP   CR+I++CC
Sbjct: 1   MGSFSLKAAAHVVFVVVLGIVLPLQTAEGFTGGKEQREFDYFVLALQWPAALCRNITVCC 60

Query: 61  QENACCAGADTSKEFTIHGLWPEYEWNGWPQCCTDAIFNENEINPLMDALDKYWPTYRCG 120
             NACC GAD+  EFTIHGLWP+YE  GWP CCT+  FNENEI+ L + LDKYWP+YRCG
Sbjct: 61  PTNACCRGADSPTEFTIHGLWPQYEGKGWPSCCTNETFNENEISSLFEDLDKYWPSYRCG 120

Query: 121 SEPTCSDRTGNFWAQQYEKHGTCASPVINSQLEYFNTTLELFSKYNLNKALQNAEIVPSD 180
              +C  R G+FWA QYEKHGTC +PVI  + +YF TTL LF KYN+ KAL+NAEI+ SD
Sbjct: 121 LVGSCDSRKGSFWAHQYEKHGTCGTPVILQEYDYFLTTLTLFFKYNVTKALENAEIIASD 180

Query: 181 TATYPKKDVIDVVTTVLQGGKPQLRCAEEGIVKEVWLCYDKLFLPRDCNPIKETCPDSVK 239
              YP +DV++ V    +   P+L CA+ GI+KE++LC+DK F  RDC+  K +CP +VK
Sbjct: 181 IKKYPIQDVVNAVHLAFK-ANPKLACAKRGIIKEIYLCFDKQFKLRDCDATK-SCPKAVK 240

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BLAST of Lag0019780 vs. ExPASy Swiss-Prot
Match: P42814 (Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 2.2e-52
Identity = 101/243 (41.56%), Postives = 139/243 (57.20%), Query Frame = 0


Query: 8   AAAAAVLVAVAVVAQLQAARFAAD-------QKEFDYFVLSLQWPGNSCRDISMCCQENA 67
           A+   +L+ VA +    A  FA D       Q+EFDYF LSLQWPG  CR    CC +NA
Sbjct: 2   ASRLCLLLLVACI----AGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNA 61

Query: 68  CCAGADTSKEFTIHGLWPEYEWNGWPQCCTDAIFNENEINPLMDALDKYWPTYRCGSEPT 127
           CC G+D   +FTIHGLWP+Y    WP CC  + F E EI+ LMD L+KYWP+  CGS  +
Sbjct: 62  CCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSS 121

Query: 128 CSDRTGNFWAQQYEKHGTCASPVINSQLEYFNTTLELFSKYNLNKALQNAEIVPSDTATY 187
           C+   G+FW  ++EKHGTC+SPV + +  YF TTL L+ K+N+   L  A  V S++  Y
Sbjct: 122 CNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFLTTLNLYLKHNVTDVLYQAGYVASNSEKY 181

Query: 188 PKKDVIDVVTTVLQGGKPQLRCAEEGIVKEVWLCYDKLFLPRDC-----NPIKETCPDSV 239
           P   ++  +        P++ C  + I  E+ +C+ K F PRDC        +++CP  V
Sbjct: 182 PLGGIVTAIQNAFH-ITPEVVCKRDAI-DEIRICFYKDFKPRDCVGSQDLTSRKSCPKYV 238

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BLAST of Lag0019780 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 4.1e-27
Identity = 75/207 (36.23%), Postives = 103/207 (49.76%), Query Frame = 0


Query: 15  VAVAVVAQLQAARFAADQKEFDYFVLSLQWPGNSCRDISMCCQENACCAGADTSKEFTIH 74
           + +  + QL    FA D   FD+F   LQWPG  C     CC        AD    F IH
Sbjct: 5   IFILALQQLYVQSFAQD---FDFFYFVLQWPGAYCDSRHSCCYPQTGKPAAD----FGIH 64

Query: 75  GLWPEYEWNGWPQCCT-DAIFNENEINPLMDALDKYWPTYRCGSEPTCSDRTGNFWAQQY 134
           GLWP Y+  GWPQ C  D+ F++  ++ LM  L + WPT  C S          FW  ++
Sbjct: 65  GLWPNYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGM-----KFWTHEW 124

Query: 135 EKHGTCASPVINSQLEYFNTTLELFSKYNLNKALQNAEIVPSDTATYPKKDVIDVVTTVL 194
           EKHGTCA   ++ Q +YF   L+L  K NL  AL NA I P D   Y  KD+ + +  V+
Sbjct: 125 EKHGTCAESELD-QHDYFEAGLKLKQKANLLHALTNAGIKPDD-KFYEMKDIENTIKQVV 184

Query: 195 QGGKPQLRCAEEGI----VKEVWLCYD 217
            G  P + C ++      + +++LC D
Sbjct: 185 -GFAPGIECNKDSSHNSQLYQIYLCVD 196

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BLAST of Lag0019780 vs. ExPASy Swiss-Prot
Match: Q7M438 (Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3)

HSP 1 Score: 117.1 bits (292), Expect = 2.9e-25
Identity = 75/234 (32.05%), Postives = 118/234 (50.43%), Query Frame = 0


Query: 8   AAAAAVLVAVAVVAQLQAARFAADQKEFDYFVLSLQWPGNSCRDISMCCQENACCAGADT 67
           AA  +VL+  +          ++   +FD+++   QW  + C D   C Q          
Sbjct: 5   AALLSVLLIASTAQSTVTIYESSKPGDFDFYLFVQQWIYSYC-DSQTCIQNK-------E 64

Query: 68  SKEFTIHGLWPEYEWNGWPQCCTDAIFNENEINPLMDALDKYWPTYRCGSEPTCSDRTGN 127
            + FTIHGLWPE     +P  C+   FN N I  L D L+  W        P+ +    +
Sbjct: 65  REAFTIHGLWPENSDGSYPSFCSGPSFNVNAIQDLEDQLNFDW--------PSLTGPNTD 124

Query: 128 FWAQQYEKHGTCA-SPVINSQLEYFNTTLELFSKYNLNKALQNAEIVPSDTATYPKKDVI 187
           FW  ++ KHGTC+ +  I    +YF T ++L++++N+  AL++  I PSD+ TY   D+ 
Sbjct: 125 FWTHEFSKHGTCSITGPITDIHDYFATGIKLYTEFNITAALESENIYPSDSNTYKPVDIT 184

Query: 188 DVVTTVLQGGKPQLRCAEEGIVKEVWLCYDKLFLP-RDCNPIKE-TCPDSVKFP 239
           + +TT   GGKP ++C+  G +  V +C DK  L   DC  ++  +C  SVKFP
Sbjct: 185 NAITTHF-GGKPGIQCS-SGQLSTVAVCIDKNSLSIMDCPDLQGWSCSGSVKFP 220

[Close]

BLAST of Lag0019780 vs. ExPASy Swiss-Prot
Match: Q7M456 (Ribonuclease Oy OS=Crassostrea gigas OX=29159 PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 6.8e-22
Identity = 68/222 (30.63%), Postives = 107/222 (48.20%), Query Frame = 0


Query: 33  KEFDYFVLSLQWPGNSCRDISMCCQENACCAGADTSKEFTIHGLWP--EYEWNGWPQCCT 92
           K+++YF  + QWP      I++C +  +C    D+   + IHGLWP  + E  G   C  
Sbjct: 1   KDWNYFTFAQQWP------IAVCAEHKSCFI-PDSVVGWGIHGLWPSSDTESKGPENCNG 60

Query: 93  DAIFNENEINPLMDALDKYWPTYRCGSEPTCSDRTGNFWAQQYEKHGTCAS--PVINSQL 152
              F+ N + PL+  L KYWP     +      +  +FW  ++ KHGTCA+  P  +++L
Sbjct: 61  SWPFDINNVMPLVPELKKYWPNLYPDT------KANSFWEHEWSKHGTCATSLPATSNEL 120

Query: 153 EYFNTTLELFSKYNLNKALQNAEIVPSDTATYPKKDVIDVVTTVLQGGKPQLRC------ 212
           +YF   L+L +KYN+++ L N  I+PS TA Y   +    V   L G    + C      
Sbjct: 121 KYFGMGLKLHAKYNISRILVNQGILPSKTAGYMINETEAAVKREL-GVDAVIECVYDKEK 180

Query: 213 AEEGIVKEVWLCYDKLFLPRDCNP---IKETCPDSVKFPIAP 242
            ++ ++ E+ +C  K F    CN     + TCP    F   P
Sbjct: 181 TKKQLLYEISICLTKEFELISCNKKEVSETTCPRKEPFFYPP 208

[Close]

BLAST of Lag0019780 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 102.4 bits (254), Expect = 7.5e-21
Identity = 68/221 (30.77%), Postives = 114/221 (51.58%), Query Frame = 0


Query: 1   MASLSFKAAAAAVLVAVAVVAQLQAARFAADQKEFDYFVLSLQWPGNSCRDISMCCQENA 60
           MAS S  +    +L+    ++ L AA      K+FD+F    QWPG+ C     CC    
Sbjct: 1   MASNSAFSLFLILLIITQCLSVLNAA------KDFDFFYFVQQWPGSYCDTKQSCCYPTT 60

Query: 61  CCAGADTSKEFTIHGLWPEYEWNGWP-QCCTDAIFNENEINPLMDALDKYWPTYRCGSEP 120
               AD    F IHGLWP      +P  C  ++ +++++I+ L+ ++ + WPT  C   P
Sbjct: 61  GKPAAD----FGIHGLWPNNNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLAC---P 120

Query: 121 TCSDRTGNFWAQQYEKHGTCASPVINSQLEYFNTTLELFSKYNLNKALQNAEIVPSDTAT 180
           + S  T  FW+ ++EKHGTCA  V+ +Q  YF   L+L ++ +L   LQ A+I P D  +
Sbjct: 121 SGSGST--FWSHEWEKHGTCAESVLTNQHAYFKKALDLKNQIDLLSILQGADIHP-DGES 180

Query: 181 YPKKDVIDVVTTVLQGGKPQLRC----AEEGIVKEVWLCYD 217
           Y   ++ + + + + G  P ++C    +    + +V++C D
Sbjct: 181 YDLVNIRNAIKSAI-GYTPWIQCNVDQSGNSQLYQVYICVD 204

[Close]

BLAST of Lag0019780 vs. ExPASy TrEMBL
Match: A0A0A0L0P9 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_4G109540 PE=3 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 3.4e-77
Identity = 137/261 (52.49%), Postives = 177/261 (67.82%), Query Frame = 0


Query: 1   MASLSFKAAAAAVLVAV-----AVVAQLQA-ARFAADQKEFDYFVLSLQWPGNSCRDISM 60
           MASLS KA A   +V V      VV    A A  +++Q+EFDYFVL+LQWPG+SC++   
Sbjct: 1   MASLSIKAKAMVFVVVVLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGK 60

Query: 61  CCQENACCAGADTSKEFTIHGLWPEYEWNGWPQCCTDAIFNENEINPLMDALDKYWPTYR 120
           CC  NACC GA++  EFTIHGLWP+Y   GWP CCTDA FNENEIN L + + KYWPTYR
Sbjct: 61  CCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYR 120

Query: 121 CGSEPTCSDRTGNFWAQQYEKHGTCASPVINSQLEYFNTTLELFSKYNLNKALQNAEIVP 180
           CG+  TC    G+FWA +YEKHGTCA+PVI  + +YF TT+ +FSKYN+ K L +A  V 
Sbjct: 121 CGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVA 180

Query: 181 SDTATYPKKDVIDVVTTVLQGGKPQLRCAEEGIVKEVWLCYDKLFLPRDCNPIKETCPDS 240
           S+T  YP +DV+  +        P++ CA++G VKE+WLC+DK F PRDCN +  +CPD 
Sbjct: 181 SNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCN-LPNSCPDF 240

Query: 241 VKFPIAPTRGPCLSNGALPWM 256
           +KFP    + P    GA+PW+
Sbjct: 241 IKFPTYEPQEPNKVEGAIPWI 260

[Close]

BLAST of Lag0019780 vs. ExPASy TrEMBL
Match: A0A6J1CHM7 (Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111011505 PE=3 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.9e-76
Identity = 134/251 (53.39%), Postives = 174/251 (69.32%), Query Frame = 0


Query: 5   SFKAAAAAVLVAVAVVAQLQAARFAADQKEFDYFVLSLQWPGNSCRDISMCCQENACCAG 64
           S   AA A  V V  V QLQ A FA DQ+EFDYFVL+LQWPG++C++IS CCQ+N CC G
Sbjct: 8   SNSVAAVAAAVFVVAVVQLQTAAFAKDQREFDYFVLALQWPGSACKNISYCCQDNGCCRG 67

Query: 65  ADTSKEFTIHGLWPEYEWNGWPQCCTDAIFNENEINPLMDALDKYWPTYRCGSEPTCSDR 124
           + T  +FTIHGLWP+YE  GWP CCTD  FNENEI  L   L + WP+YRCGS  +C+++
Sbjct: 68  SQTPTQFTIHGLWPQYEVKGWPSCCTDESFNENEIFVLRKILPEIWPSYRCGSVTSCNNK 127

Query: 125 TGNFWAQQYEKHGTCASPVINSQLEYFNTTLELFSKYNLNKALQNAEIVPSDTATYPKKD 184
            G+FWA++YEKHGTCA+PVI  Q  YF+T + LF+ +N+ KAL+NA IV SDT  YP  D
Sbjct: 128 KGSFWAEEYEKHGTCAAPVIEGQYNYFSTAIGLFNTHNVTKALENAGIVASDTVKYPLND 187

Query: 185 VIDVVTTVLQGGKPQLRCAEEGIVKEVWLCYDKLFLPRDCNPIKETCPDSVKFPIAPTRG 244
           V+  V     GG P++ C ++GI+KE++LC+DK F PR+C+  K +CP  VK P      
Sbjct: 188 VMAAVEFAF-GGTPKIMCGKKGIIKELYLCFDKKFKPRNCDETK-SCPKKVKLPTIEQLE 247

Query: 245 PCLSNGALPWM 256
             +  GA+P M
Sbjct: 248 SYMGEGAIPRM 256

[Close]

BLAST of Lag0019780 vs. ExPASy TrEMBL
Match: A0A1S3CHX1 (Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103501148 PE=3 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 3.9e-73
Identity = 130/260 (50.00%), Postives = 167/260 (64.23%), Query Frame = 0


Query: 1   MASLSFKAAAAAVLVAV----AVVAQLQA-ARFAADQKEFDYFVLSLQWPGNSCRDISMC 60
           MAS S K      L  +     VV    A A   ++Q+EFDYFVL+LQWP  SC++   C
Sbjct: 1   MASFSIKGKPMVFLFLIVELLTVVFGFSAEAPSGSEQREFDYFVLALQWPATSCKNAGKC 60

Query: 61  CQENACCAGADTSKEFTIHGLWPEYEWNGWPQCCTDAIFNENEINPLMDALDKYWPTYRC 120
           C  NACC GAD+  EFTIHGLWP+Y   GWP CCTDA FNENEI  L + + KYWPTYRC
Sbjct: 61  CPSNACCRGADSPTEFTIHGLWPQYNEKGWPSCCTDASFNENEIGILAEDIQKYWPTYRC 120

Query: 121 GSEPTCSDRTGNFWAQQYEKHGTCASPVINSQLEYFNTTLELFSKYNLNKALQNAEIVPS 180
           G+  TC    G+FWA +YEKHGTCA+PV+  + +YF T L +FSKYN+ K L     V S
Sbjct: 121 GTSSTCHRTKGSFWAHEYEKHGTCAAPVLVGEYDYFLTALTIFSKYNVTKVLSEGGFVAS 180

Query: 181 DTATYPKKDVIDVVTTVLQGGKPQLRCAEEGIVKEVWLCYDKLFLPRDCNPIKETCPDSV 240
           +T  YP +DVI  +        P++ CA++G+VKE+WLC+DK F PRDCN ++ +CPD +
Sbjct: 181 NTEKYPIEDVIAAIKNKFYNATPKITCAKKGVVKELWLCFDKSFEPRDCN-LQHSCPDFI 240

Query: 241 KFPIAPTRGPCLSNGALPWM 256
           K P    + P    GA+PW+
Sbjct: 241 KLPTYEPQEPNKVEGAIPWI 259

[Close]

BLAST of Lag0019780 vs. ExPASy TrEMBL
Match: A0A6J1CGP7 (Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111011504 PE=3 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 8.8e-73
Identity = 137/263 (52.09%), Postives = 170/263 (64.64%), Query Frame = 0


Query: 1   MASLSFKA-AAAAVLVAVAVVAQLQAARFAA-------DQKEFDYFVLSLQWPGNSCRDI 60
           MAS S K    AA  V + VV QLQAA FAA       +Q+EFDYFVL+LQWP  SC + 
Sbjct: 1   MASSSIKGNPTAAATVFLLVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTNP 60

Query: 61  SMCCQENACCAGADTSKEFTIHGLWPEYEWNGWPQCCTDAIFNENEINPLMDALDKYWPT 120
             CC  N CC GA++  EFTIHGLWP+YE  GWP CC+DA F+ENEI  L   + KYWPT
Sbjct: 61  GKCCPSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYWPT 120

Query: 121 YRCGSEPTCSDRTGNFWAQQYEKHGTCASPVINSQLEYFNTTLELFSKYNLNKALQNAEI 180
           YRCG+  TC    G+FWA +YEKHGTCA+PVI  + +YF TTL +FSKYN+ K L +A  
Sbjct: 121 YRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAGF 180

Query: 181 VPSDTATYPKKDVIDVVTTVLQGGKPQLRCAEEGIVKEVWLCYDKLFLPRDCNPIKETCP 240
           V SDT  Y   DV+  V        P++ CA++G VKE+WLC+DK F PRDC+ +  +CP
Sbjct: 181 VASDTEKYALGDVVAAVQDAF-NATPKISCAKKGAVKELWLCFDKTFQPRDCD-LANSCP 240

Query: 241 DSVKFPIAPTRGPCLSNGALPWM 256
           D +K P    +      GA+PWM
Sbjct: 241 DFIKLPTYEPQEANKVEGAIPWM 261

[Close]

BLAST of Lag0019780 vs. ExPASy TrEMBL
Match: A0A6J1J670 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111482949 PE=3 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 2.0e-72
Identity = 128/251 (51.00%), Postives = 171/251 (68.13%), Query Frame = 0


Query: 8   AAAAAVLVAVAVVAQLQAARF---AADQKEFDYFVLSLQWPGNSCRDISMCCQENACCAG 67
           A A  +L+A+ +VA + AA        QKEFDYFVL+LQWP  SC++   CC  N CC G
Sbjct: 14  APAVFLLLALQLVAVVLAAETPSGGGGQKEFDYFVLALQWPATSCKNTGKCCPANGCCRG 73

Query: 68  ADTSKEFTIHGLWPEYEWNGWPQCCTDAIFNENEINPLMDALDKYWPTYRCGSEPTCSDR 127
           AD+  EFTIHGLWP+Y   GWP CCTDA+FN+NEI+ L + ++KYWPTYRCG+   C   
Sbjct: 74  ADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLSEDIEKYWPTYRCGTTSNCHGT 133

Query: 128 TGNFWAQQYEKHGTCASPVINSQLEYFNTTLELFSKYNLNKALQNAEIVPSDTATYPKKD 187
            G+FWA +YEKHGTCA+PVI  + +YF TTL +FSKYN+ KAL +A  V S+T  YP  D
Sbjct: 134 KGSFWAHEYEKHGTCAAPVIVGEYDYFLTTLTIFSKYNVTKALSDAGFVASNTEKYPLGD 193

Query: 188 VIDVVTTVLQGGKPQLRCAEEGIVKEVWLCYDKLFLPRDCNPIKETCPDSVKFPIAPTRG 247
           V+D V        P++ CA++G VKE+W+C+DK F PRDC+ +  +CP+ +K P    +G
Sbjct: 194 VVDAVKNA-TNSTPKISCAKKGAVKELWICFDKNFEPRDCD-LANSCPEFIKLPTYEPQG 253

Query: 248 PCLSNGALPWM 256
             +  GA+PW+
Sbjct: 254 --VVEGAIPWI 260

[Close]

BLAST of Lag0019780 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 207.2 bits (526), Expect = 1.5e-53
Identity = 101/243 (41.56%), Postives = 139/243 (57.20%), Query Frame = 0


Query: 8   AAAAAVLVAVAVVAQLQAARFAAD-------QKEFDYFVLSLQWPGNSCRDISMCCQENA 67
           A+   +L+ VA +    A  FA D       Q+EFDYF LSLQWPG  CR    CC +NA
Sbjct: 2   ASRLCLLLLVACI----AGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNA 61

Query: 68  CCAGADTSKEFTIHGLWPEYEWNGWPQCCTDAIFNENEINPLMDALDKYWPTYRCGSEPT 127
           CC G+D   +FTIHGLWP+Y    WP CC  + F E EI+ LMD L+KYWP+  CGS  +
Sbjct: 62  CCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSS 121

Query: 128 CSDRTGNFWAQQYEKHGTCASPVINSQLEYFNTTLELFSKYNLNKALQNAEIVPSDTATY 187
           C+   G+FW  ++EKHGTC+SPV + +  YF TTL L+ K+N+   L  A  V S++  Y
Sbjct: 122 CNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFLTTLNLYLKHNVTDVLYQAGYVASNSEKY 181

Query: 188 PKKDVIDVVTTVLQGGKPQLRCAEEGIVKEVWLCYDKLFLPRDC-----NPIKETCPDSV 239
           P   ++  +        P++ C  + I  E+ +C+ K F PRDC        +++CP  V
Sbjct: 182 PLGGIVTAIQNAFH-ITPEVVCKRDAI-DEIRICFYKDFKPRDCVGSQDLTSRKSCPKYV 238

[Close]

BLAST of Lag0019780 vs. TAIR 10
Match: AT2G39780.2 (ribonuclease 2 )

HSP 1 Score: 151.8 bits (382), Expect = 7.7e-37
Identity = 84/243 (34.57%), Postives = 115/243 (47.33%), Query Frame = 0


Query: 8   AAAAAVLVAVAVVAQLQAARFAAD-------QKEFDYFVLSLQWPGNSCRDISMCCQENA 67
           A+   +L+ VA +    A  FA D       Q+EFDYF LSLQWPG  CR    CC +NA
Sbjct: 2   ASRLCLLLLVACI----AGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNA 61

Query: 68  CCAGADTSKEFTIHGLWPEYEWNGWPQCCTDAIFNENEINPLMDALDKYWPTYRCGSEPT 127
           CC G+D   +FTIHGLWP+Y    WP CC  + F E EI+ LMD L+KYWP+  CGS  +
Sbjct: 62  CCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSS 121

Query: 128 CSDRTGNFWAQQYEKHGTCASPVINSQLEYFNTTLELFSKYNLNKALQNAEIVPSDTATY 187
           C+   G+FW  +                                  L  A  V S++  Y
Sbjct: 122 CNGGKGSFWGHE--------------------------------DVLYQAGYVASNSEKY 181

Query: 188 PKKDVIDVVTTVLQGGKPQLRCAEEGIVKEVWLCYDKLFLPRDC-----NPIKETCPDSV 239
           P   ++  +        P++ C  + I  E+ +C+ K F PRDC        +++CP  V
Sbjct: 182 PLGGIVTAIQNAFH-ITPEVVCKRDAI-DEIRICFYKDFKPRDCVGSQDLTSRKSCPKYV 206

[Close]

BLAST of Lag0019780 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 123.2 bits (308), Expect = 2.9e-28
Identity = 75/207 (36.23%), Postives = 103/207 (49.76%), Query Frame = 0


Query: 15  VAVAVVAQLQAARFAADQKEFDYFVLSLQWPGNSCRDISMCCQENACCAGADTSKEFTIH 74
           + +  + QL    FA D   FD+F   LQWPG  C     CC        AD    F IH
Sbjct: 5   IFILALQQLYVQSFAQD---FDFFYFVLQWPGAYCDSRHSCCYPQTGKPAAD----FGIH 64

Query: 75  GLWPEYEWNGWPQCCT-DAIFNENEINPLMDALDKYWPTYRCGSEPTCSDRTGNFWAQQY 134
           GLWP Y+  GWPQ C  D+ F++  ++ LM  L + WPT  C S          FW  ++
Sbjct: 65  GLWPNYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGM-----KFWTHEW 124

Query: 135 EKHGTCASPVINSQLEYFNTTLELFSKYNLNKALQNAEIVPSDTATYPKKDVIDVVTTVL 194
           EKHGTCA   ++ Q +YF   L+L  K NL  AL NA I P D   Y  KD+ + +  V+
Sbjct: 125 EKHGTCAESELD-QHDYFEAGLKLKQKANLLHALTNAGIKPDD-KFYEMKDIENTIKQVV 184

Query: 195 QGGKPQLRCAEEGI----VKEVWLCYD 217
            G  P + C ++      + +++LC D
Sbjct: 185 -GFAPGIECNKDSSHNSQLYQIYLCVD 196

[Close]

BLAST of Lag0019780 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 122.9 bits (307), Expect = 3.8e-28
Identity = 73/217 (33.64%), Postives = 111/217 (51.15%), Query Frame = 0


Query: 32  QKEFDYFVLSLQWPGNSCRDISMCCQENACCAGADTSKEFTIHGLWPEYEWNGWP-QCCT 91
           Q++FD+F   LQWPG  C     CC   +    AD    F IHGLWP Y    WP  C  
Sbjct: 25  QEDFDFFYFVLQWPGAYCDTSRACCYPTSGKPAAD----FGIHGLWPNYNGGSWPSNCDP 84

Query: 92  DAIFNENEINPLMDALDKYWPTYRCGSEPTCSDRTGNFWAQQYEKHGTCASPVINSQLEY 151
           D+ F+ ++I+ L+ +L K WPT  C      S+   NFW  ++EKHGTC+  V++ Q EY
Sbjct: 85  DSQFDRSQISDLVSSLKKNWPTLSCP-----SNEGFNFWEHEWEKHGTCSESVMD-QHEY 144

Query: 152 FNTTLELFSKYNLNKALQNAEIVPSDTATYPKKDVIDVVTTVLQGG---KPQLRCAEE-- 211
           F   L+L  K NL + L+N+ I P D   Y     +D +T  ++ G    P + C ++  
Sbjct: 145 FENALKLKQKANLLQILKNSGINPDD-GFYN----LDKITNAIKDGIGFTPGIECNKDPE 204

Query: 212 --GIVKEVWLCYDKL---FLPRDCNPIKETCPDSVKF 238
               + ++++C D     F+     P + +CP  ++F
Sbjct: 205 RNAQLHQIYICVDTSGTEFIECPVLP-RGSCPSQIQF 225

[Close]

BLAST of Lag0019780 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 113.6 bits (283), Expect = 2.3e-25
Identity = 69/194 (35.57%), Postives = 100/194 (51.55%), Query Frame = 0


Query: 29  AADQKEFDYFVLSLQWPGNSCRDISMCCQENACCAGADTSKEFTIHGLWPEYEWNGWP-Q 88
           ++  ++FD+F    QWPG+ C     CC  N+    AD    F IHGLWP Y+   +P  
Sbjct: 24  SSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAAD----FGIHGLWPNYKDGTYPSN 83

Query: 89  CCTDAIFNENEINPLMDALDKYWPTYRCGSEPTCSDRTGNFWAQQYEKHGTCASPVINSQ 148
           C     F+ + I+ L+ ++ K WPT  C   P+ S     FW  ++EKHGTC+  VI+ Q
Sbjct: 84  CDASKPFDSSTISDLLTSMKKSWPTLAC---PSGSGEA--FWEHEWEKHGTCSESVID-Q 143

Query: 149 LEYFNTTLELFSKYNLNKALQNAEIVPSDTATYPKKDVIDVVTTVLQGGKPQLRCAEEGI 208
            EYF T L L  K NL  AL  A I P D  +Y  + + D +   + G  P + C  +G 
Sbjct: 144 HEYFQTALNLKQKTNLLGALTKAGINP-DGKSYSLESIRDSIKESI-GFTPWVECNRDGS 203

Query: 209 ----VKEVWLCYDK 218
               + +V+LC D+
Sbjct: 204 GNSQLYQVYLCVDR 205

The following BLAST results are available for this feature:

BLAST of Lag0019780 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137162.1	7.1e-77	52.49	ribonuclease 2 [Cucumis sativus]	[more]
XP_022140986.1	6.0e-76	53.39	ribonuclease 2-like [Momordica charantia]	[more]
KAE8649307.1	9.6e-74	54.10	hypothetical protein Csa_015411 [Cucumis sativus]	[more]
XP_008462866.1	8.1e-73	50.00	PREDICTED: ribonuclease 2-like [Cucumis melo]	[more]
XP_038876678.1	1.1e-72	55.79	ribonuclease 2-like isoform X1 [Benincasa hispida]	[more]

BLAST of Lag0019780 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P42814	2.2e-52	41.56	Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1	[more]
P42815	4.1e-27	36.23	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
Q7M438	2.9e-25	32.05	Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3	[more]
Q7M456	6.8e-22	30.63	Ribonuclease Oy OS=Crassostrea gigas OX=29159 PE=1 SV=1	[more]
P80022	7.5e-21	30.77	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]

BLAST of Lag0019780 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L0P9	3.4e-77	52.49	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_4G109540 PE=3 SV=1	[more]
A0A6J1CHM7	2.9e-76	53.39	Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111011505 PE=3 SV=1	[more]
A0A1S3CHX1	3.9e-73	50.00	Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103501148 PE=3 SV=1	[more]
A0A6J1CGP7	8.8e-73	52.09	Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111011504 PE=3 SV=1	[more]
A0A6J1J670	2.0e-72	51.00	Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111482949 PE=3 SV=1	[more]

BLAST of Lag0019780 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39780.1	1.5e-53	41.56	ribonuclease 2	[more]
AT2G39780.2	7.7e-37	34.57	ribonuclease 2	[more]
AT1G26820.1	2.9e-28	36.23	ribonuclease 3	[more]
AT1G14220.1	3.8e-28	33.64	Ribonuclease T2 family protein	[more]
AT2G02990.1	2.3e-25	35.57	ribonuclease 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 35..224 e-value: 1.8E-44 score: 152.1
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 15..228
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 31..241 e-value: 2.7E-67 score: 228.8
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 34..239
None	No IPR available	PANTHER	PTHR11240:SF51	RIBONUCLEASE 2	coord: 15..228
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 71..78
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 35..237 e-value: 2.73622E-59 score: 184.458

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0019780.1	Lag0019780.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006401	RNA catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016020	membrane
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding