Lag0019761 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0019761
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationchr5: 45193876 .. 45195978 (+)
RNA-Seq ExpressionLag0019761
SyntenyLag0019761
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAGCGTTAAAGCCATCGCTGAAACACCCAACTTAGCCTCCATTCCTTCCTCCTACATCTTCTCCCCCGACGAGCCTGACGGTGTCGCCGCCGATGCAGCTCCACACGGCGTTGAAGTTTCGATCCCCACCATTGATTTTTCTCTCCTCACCGATGGTACTCCCTGTCAACGAGCCAAAGTCGTCGATGAGCTCGGCAAGGCCTGCCAGGATTGGGGCTTCTTTATGGTATGTGTGTATGAAACTTCGTTAAACTACTAATAAATTTAAAAGTTTAGCACTCGTTTACACTTGAGCTACAAATAGGGTTTGACAGCTTTCAGAATTCCTGTTCTGATACCATGTTAAATTATTGTTGGACGACTCAAAAGTTCAAAGCGATTTTTTTTTCTTTTTTATTTATATTCTTAACAAACCCGTCTGCGAAAACGTGATTTTAACGGAGATTAACGACGTGTGAGGGATGATGTAGGTGATCAATCATGGAGTGGAAGAGAGGGTGATGAAGGAAATGGTGGAAATTTGCAGAGAATTTTTTGATCAAAAGGAGGAGGAGAAGAGAGAGTATGAAACAAAAGATGTTCTTGATCCCATCAGATATGGCACAAGCTTCAATCCTAAGATGGAGAAGGTTTTCTTTTGGAGAGATTATTTGAAAATCTTGGTTCATCCCAAGTTTCATTCCCCAACTAAACCTCCAAAATTCAGGTCAGCCCTAAGTTTTTATTATTGAACATATTCCTTTGAGAAAGCAAAAGCTCTTGTTTTAATTGTGAAAGAAAAACAAAAACTTGGTGGACACCATTCAATGGTTTTATTTATTGATAATCACATAATTATCTTCATGGTTTTTCTTTCATTGATATTTACCTGTATTAATGCATGTGAGTGAAAGGACAGATTATATGGATGAAACAACATCATAGCAGATTGATAAAAGATATTTCAATCTCTAAAGAAATTTTTTTAGGAAGCATATGACTGATGACAGTAAATTGAAAGAACTTTACGTTGGTCTGTAAGAGAAATCTTCAATTTTAAATGCAACTCAAGAGATTCGTATAGGTTGTAGAGATATGTCAAGACTAATGTTGAATTCACATTTCAAATTTTAGTCGGCGCAGATTGTTTTATACAACCAAACATCATAAAAAAAATTAAAATGTTTTGTACTTAATGAAATAGTACTTTTCACCGTTTTAAAAGTTATACCAGGTCGCCTTTTAAGCAAAGTTTATGAACTAAAGTAGAATTTGTTCTCTTTGTTTTATCATTGTAGAGAGATCTTGGAAGAGTATTGCAAAAGAATCAGAGAAATGAGCAGAGAATTGGTGAGAGGAATCTCAGAGAGCTTAGGATTGGAAGGCAGTAATTTGGAAAAAGCAGTTGATCTTGAATCATGTTCAATACTATTTGCAGCAAATTTGTATCCACCATGTCCACAGCCAGAGCTTGCAAGGGGCTTGCCATCTCACTCTGATCAGTGCCTTCTCACTCTTCTTCTTCAAAACAACATTGGTGGCCTTCAAATCTTACATGATCATAACTGGCTCAATGTCAATCCCATTCCCAACTCATTTCTTGTTAATGTTGCTGATCAACTTGAGGTACACACCCAAATATTTCCTTTTCTCCATTTCTTCCTTACAATCACACCATTTTCGAATGAATTTTTTTACGTAACCTCGATTTAACTCGTCCTGATATAAGTCAAATAATTGAAAGTTTAGAAACCAGAATGAAATAGGTATTTGAATATGTTTTAGAAATGGGTTTTGAATCGGGTTTCGGACCGACCAAACCCGCGGAATGGTTTCGTGAACTTGATATGTTCATTGGGTGTGTTAGATTTTGAGCAATGGGAAGTACAAGAGTGTTTTGCACAGAGCAATGGTGAATGCTAAGGCCACAAGGATATCCATAGCCATGGCAGTTGGGCCATCACCTGAAACAGTGGTTGGTCCTGTGGAAGAACTGGTACATAAACAAGCCAATAATCCTCCATTGTTTAAGCACATCAAATACAGAGATTATATGGAAATGATGCAGAGCAACCAACTGCAAGGGAAATCCAGCTTGGATCGTATTCGACTTCTTTGA

mRNA sequence

ATGGCCAGCGTTAAAGCCATCGCTGAAACACCCAACTTAGCCTCCATTCCTTCCTCCTACATCTTCTCCCCCGACGAGCCTGACGGTGTCGCCGCCGATGCAGCTCCACACGGCGTTGAAGTTTCGATCCCCACCATTGATTTTTCTCTCCTCACCGATGGTACTCCCTGTCAACGAGCCAAAGTCGTCGATGAGCTCGGCAAGGCCTGCCAGGATTGGGGCTTCTTTATGGTGATCAATCATGGAGTGGAAGAGAGGGTGATGAAGGAAATGGTGGAAATTTGCAGAGAATTTTTTGATCAAAAGGAGGAGGAGAAGAGAGAGTATGAAACAAAAGATGTTCTTGATCCCATCAGATATGGCACAAGCTTCAATCCTAAGATGGAGAAGGTTTTCTTTTGGAGAGATTATTTGAAAATCTTGGTTCATCCCAAGTTTCATTCCCCAACTAAACCTCCAAAATTCAGAGAGATCTTGGAAGAGTATTGCAAAAGAATCAGAGAAATGAGCAGAGAATTGGTGAGAGGAATCTCAGAGAGCTTAGGATTGGAAGGCAGTAATTTGGAAAAAGCAGTTGATCTTGAATCATGTTCAATACTATTTGCAGCAAATTTGTATCCACCATGTCCACAGCCAGAGCTTGCAAGGGGCTTGCCATCTCACTCTGATCAGTGCCTTCTCACTCTTCTTCTTCAAAACAACATTGGTGGCCTTCAAATCTTACATGATCATAACTGGCTCAATGTCAATCCCATTCCCAACTCATTTCTTGTTAATGTTGCTGATCAACTTGAGATTTTGAGCAATGGGAAGTACAAGAGTGTTTTGCACAGAGCAATGGTGAATGCTAAGGCCACAAGGATATCCATAGCCATGGCAGTTGGGCCATCACCTGAAACAGTGGTTGGTCCTGTGGAAGAACTGGTACATAAACAAGCCAATAATCCTCCATTGTTTAAGCACATCAAATACAGAGATTATATGGAAATGATGCAGAGCAACCAACTGCAAGGGAAATCCAGCTTGGATCGTATTCGACTTCTTTGA

Coding sequence (CDS)

ATGGCCAGCGTTAAAGCCATCGCTGAAACACCCAACTTAGCCTCCATTCCTTCCTCCTACATCTTCTCCCCCGACGAGCCTGACGGTGTCGCCGCCGATGCAGCTCCACACGGCGTTGAAGTTTCGATCCCCACCATTGATTTTTCTCTCCTCACCGATGGTACTCCCTGTCAACGAGCCAAAGTCGTCGATGAGCTCGGCAAGGCCTGCCAGGATTGGGGCTTCTTTATGGTGATCAATCATGGAGTGGAAGAGAGGGTGATGAAGGAAATGGTGGAAATTTGCAGAGAATTTTTTGATCAAAAGGAGGAGGAGAAGAGAGAGTATGAAACAAAAGATGTTCTTGATCCCATCAGATATGGCACAAGCTTCAATCCTAAGATGGAGAAGGTTTTCTTTTGGAGAGATTATTTGAAAATCTTGGTTCATCCCAAGTTTCATTCCCCAACTAAACCTCCAAAATTCAGAGAGATCTTGGAAGAGTATTGCAAAAGAATCAGAGAAATGAGCAGAGAATTGGTGAGAGGAATCTCAGAGAGCTTAGGATTGGAAGGCAGTAATTTGGAAAAAGCAGTTGATCTTGAATCATGTTCAATACTATTTGCAGCAAATTTGTATCCACCATGTCCACAGCCAGAGCTTGCAAGGGGCTTGCCATCTCACTCTGATCAGTGCCTTCTCACTCTTCTTCTTCAAAACAACATTGGTGGCCTTCAAATCTTACATGATCATAACTGGCTCAATGTCAATCCCATTCCCAACTCATTTCTTGTTAATGTTGCTGATCAACTTGAGATTTTGAGCAATGGGAAGTACAAGAGTGTTTTGCACAGAGCAATGGTGAATGCTAAGGCCACAAGGATATCCATAGCCATGGCAGTTGGGCCATCACCTGAAACAGTGGTTGGTCCTGTGGAAGAACTGGTACATAAACAAGCCAATAATCCTCCATTGTTTAAGCACATCAAATACAGAGATTATATGGAAATGATGCAGAGCAACCAACTGCAAGGGAAATCCAGCTTGGATCGTATTCGACTTCTTTGA

Protein sequence

MASVKAIAETPNLASIPSSYIFSPDEPDGVAADAAPHGVEVSIPTIDFSLLTDGTPCQRAKVVDELGKACQDWGFFMVINHGVEERVMKEMVEICREFFDQKEEEKREYETKDVLDPIRYGTSFNPKMEKVFFWRDYLKILVHPKFHSPTKPPKFREILEEYCKRIREMSRELVRGISESLGLEGSNLEKAVDLESCSILFAANLYPPCPQPELARGLPSHSDQCLLTLLLQNNIGGLQILHDHNWLNVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNAKATRISIAMAVGPSPETVVGPVEELVHKQANNPPLFKHIKYRDYMEMMQSNQLQGKSSLDRIRLL
Homology
BLAST of Lag0019761 vs. NCBI nr
Match: XP_022985114.1 (protein DMR6-LIKE OXYGENASE 2-like [Cucurbita maxima])

HSP 1 Score: 560.8 bits (1444), Expect = 8.0e-156
Identity = 283/351 (80.63%), Postives = 311/351 (88.60%), Query Frame = 0

Query: 1   MASVKAIAETPNLASIPSSYIF-SPDEPDGV--AADAAPHGVEVSIPTIDFSLLTDGTPC 60
           + SVKAIAETPNLASIPSSYIF + D+ D V  AADAAPH VEVSIPTIDFSLLT GT  
Sbjct: 34  LPSVKAIAETPNLASIPSSYIFTTSDDSDDVAAAADAAPHRVEVSIPTIDFSLLTTGTSH 93

Query: 61  QRAKVVDELGKACQDWGFFMVINHGVEERVMKEMVEICREFFDQKEEEKREYETKDVLDP 120
           QR+KVV+ELGKAC DWGFFMVINHGV E +MKEMVEICREFFD KEEEKREYETK VLDP
Sbjct: 94  QRSKVVNELGKACHDWGFFMVINHGVGEGLMKEMVEICREFFDLKEEEKREYETKHVLDP 153

Query: 121 IRYGTSFNPKMEKVFFWRDYLKILVHPKFHSPTKPPKFREILEEYCKRIREMSRELVRGI 180
           IRYGTSFNPKME+VFFWRDYLK+LVHPKFHSP KP +FREILEEY KRIREM+RELVRGI
Sbjct: 154 IRYGTSFNPKMEEVFFWRDYLKVLVHPKFHSPPKPTRFREILEEYSKRIREMARELVRGI 213

Query: 181 SESLGLEGSNLEKAVDLESCSILFAANLYPPCPQPELARGLPSHSDQCLLTLLLQNNIGG 240
           SESLGLE  NLEKA +LES S LFAANLYPPCPQP+ ARGLP HSDQCLLT+LLQN++ G
Sbjct: 214 SESLGLEAYNLEKAAELESSSTLFAANLYPPCPQPQFARGLPPHSDQCLLTVLLQNHVSG 273

Query: 241 LQILHDHNWLNVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNAKATRISIAMAVGPS 300
           LQILHDH WL VNP+PN+ LVN ADQLEI+SNGKYKSVLHRAMVN KATRISIAMA+GPS
Sbjct: 274 LQILHDHQWLTVNPVPNALLVNTADQLEIMSNGKYKSVLHRAMVNDKATRISIAMAIGPS 333

Query: 301 PETVVGPVEELVHKQANNPPLFKHIKYRDYMEMMQSNQLQGKSSLDRIRLL 349
            +T+V P+ EL+HK  NNPPLFK IKY+DYMEM+QSN+L+ KS LDR+RLL
Sbjct: 334 SQTLVAPLPELLHKH-NNPPLFKTIKYKDYMEMLQSNKLERKSCLDRLRLL 383

BLAST of Lag0019761 vs. NCBI nr
Match: KAG6576847.1 (2-oxoglutarate-dependent dioxygenase 19, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 560.5 bits (1443), Expect = 1.0e-155
Identity = 282/351 (80.34%), Postives = 310/351 (88.32%), Query Frame = 0

Query: 1   MASVKAIAETPNLASIPSSYIF-SPDEPDGVA--ADAAPHGVEVSIPTIDFSLLTDGTPC 60
           + SVKAIAETPNLASIPSSYIF + D+ D VA  ADAAPH VE SIPTIDFSLLT GTP 
Sbjct: 3   LPSVKAIAETPNLASIPSSYIFTTSDDSDDVATTADAAPHSVEDSIPTIDFSLLTTGTPH 62

Query: 61  QRAKVVDELGKACQDWGFFMVINHGVEERVMKEMVEICREFFDQKEEEKREYETKDVLDP 120
           QR+KVVDELGKAC DWGFFMVINHGV E +MKEMVEICREFFD  EEEKR YETK VLDP
Sbjct: 63  QRSKVVDELGKACHDWGFFMVINHGVGEGLMKEMVEICREFFDLTEEEKRVYETKHVLDP 122

Query: 121 IRYGTSFNPKMEKVFFWRDYLKILVHPKFHSPTKPPKFREILEEYCKRIREMSRELVRGI 180
           IRYGTSFNPKME+VFFWRDYLK+LVHPKFHSP+KP +FREILEEY KRI+EM+RELVRGI
Sbjct: 123 IRYGTSFNPKMEEVFFWRDYLKVLVHPKFHSPSKPTRFREILEEYSKRIKEMARELVRGI 182

Query: 181 SESLGLEGSNLEKAVDLESCSILFAANLYPPCPQPELARGLPSHSDQCLLTLLLQNNIGG 240
           SESLGLE  NLEKA DLES S LFAANLYPPCPQP+LARGLP HSDQCLLT+LLQN++ G
Sbjct: 183 SESLGLEACNLEKAADLESSSTLFAANLYPPCPQPQLARGLPPHSDQCLLTVLLQNHVAG 242

Query: 241 LQILHDHNWLNVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNAKATRISIAMAVGPS 300
           LQILHDH WL VNP+PN  LVN ADQLEI+SNGKYKSVLHRAMVN KATRISIAMA+GPS
Sbjct: 243 LQILHDHQWLTVNPVPNVLLVNTADQLEIMSNGKYKSVLHRAMVNDKATRISIAMAIGPS 302

Query: 301 PETVVGPVEELVHKQANNPPLFKHIKYRDYMEMMQSNQLQGKSSLDRIRLL 349
            +T+V P+ EL+HK  N+PPLFK I Y+DYMEM+QSN+L+GKS LDR+RLL
Sbjct: 303 SQTLVAPLPELLHKH-NSPPLFKSIMYKDYMEMLQSNKLEGKSCLDRVRLL 352

BLAST of Lag0019761 vs. NCBI nr
Match: XP_023552481.1 (protein DMR6-LIKE OXYGENASE 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 558.1 bits (1437), Expect = 5.2e-155
Identity = 282/351 (80.34%), Postives = 310/351 (88.32%), Query Frame = 0

Query: 1   MASVKAIAETPNLASIPSSYIF-SPDEPDGVA--ADAAPHGVEVSIPTIDFSLLTDGTPC 60
           + SVKAIAETPNLASIPSSYIF + D+ D VA  ADAAPHGVE SIPTIDFSLLT GTP 
Sbjct: 29  LPSVKAIAETPNLASIPSSYIFTTSDDSDDVATTADAAPHGVEDSIPTIDFSLLTTGTPH 88

Query: 61  QRAKVVDELGKACQDWGFFMVINHGVEERVMKEMVEICREFFDQKEEEKREYETKDVLDP 120
           QR+KVV+ELGKAC DWGFFMVINHGV E +MKEMVEICREFFD  EEEKR YETK VLDP
Sbjct: 89  QRSKVVNELGKACHDWGFFMVINHGVGEGLMKEMVEICREFFDLTEEEKRVYETKHVLDP 148

Query: 121 IRYGTSFNPKMEKVFFWRDYLKILVHPKFHSPTKPPKFREILEEYCKRIREMSRELVRGI 180
           IRYGTSFNPKME+VFFWRDYLK+LVHPKFHSP KP +FREILEEY KRI+EM+RELVRGI
Sbjct: 149 IRYGTSFNPKMEEVFFWRDYLKVLVHPKFHSPPKPTRFREILEEYSKRIKEMARELVRGI 208

Query: 181 SESLGLEGSNLEKAVDLESCSILFAANLYPPCPQPELARGLPSHSDQCLLTLLLQNNIGG 240
           SESLGLE  N+EKA DLES S LFAANLYPPCPQP+LARGLP HSDQCLLT+LLQN++ G
Sbjct: 209 SESLGLEACNIEKAADLESSSTLFAANLYPPCPQPQLARGLPPHSDQCLLTVLLQNHVAG 268

Query: 241 LQILHDHNWLNVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNAKATRISIAMAVGPS 300
           LQILHDH WL VNPIPN+ LVN ADQLEI+SNGKYKSVLHRAMVN KATRISIAMA+GPS
Sbjct: 269 LQILHDHQWLTVNPIPNALLVNTADQLEIMSNGKYKSVLHRAMVNDKATRISIAMAIGPS 328

Query: 301 PETVVGPVEELVHKQANNPPLFKHIKYRDYMEMMQSNQLQGKSSLDRIRLL 349
            +T+V P+ EL+HK  N+PPLFK I Y+DYMEM+QSN+L+ KS LDRIRLL
Sbjct: 329 SQTLVAPLPELLHKH-NSPPLFKSIMYKDYMEMLQSNKLERKSCLDRIRLL 378

BLAST of Lag0019761 vs. NCBI nr
Match: XP_038904929.1 (protein DMR6-LIKE OXYGENASE 2-like [Benincasa hispida])

HSP 1 Score: 555.8 bits (1431), Expect = 2.6e-154
Identity = 275/347 (79.25%), Postives = 308/347 (88.76%), Query Frame = 0

Query: 1   MASVKAIAETPNLASIPSSYIFSPDEPDGVAADAAPHGVEVSIPTIDFSLLTDGTPCQRA 60
           MASVK++A+TPNLAS+PSS++F+ D+ D  +  A   G E SIP ID SLL DGTP QRA
Sbjct: 3   MASVKSLADTPNLASVPSSFMFATDD-DSDSVTAVSQGAEDSIPIIDTSLLIDGTPHQRA 62

Query: 61  KVVDELGKACQDWGFFMVINHGVEERVMKEMVEICREFFDQKEEEKREYETKDVLDPIRY 120
           KV++ELGKAC+DWGFFMV+NHGV ER+M+EMVEIC+EFFD KEEEKREYETK VLDPIRY
Sbjct: 63  KVINELGKACEDWGFFMVVNHGVGERLMREMVEICKEFFDLKEEEKREYETKSVLDPIRY 122

Query: 121 GTSFNPKMEKVFFWRDYLKILVHPKFHSPTKPPKFREILEEYCKRIREMSRELVRGISES 180
           GTSFNPK+EKVFFWRDYLKI+VHP FHSPTKP +FREILEEYCKRIREM+RELVRGISES
Sbjct: 123 GTSFNPKVEKVFFWRDYLKIMVHPNFHSPTKPTRFREILEEYCKRIREMTRELVRGISES 182

Query: 181 LGLEGSNLEKAVDLESCSILFAANLYPPCPQPELARGLPSHSDQCLLTLLLQNNIGGLQI 240
           LGLE   L+KA +LESCSI+FAANLYPPCPQPELARGLPSHSD CLLT+LLQN I GLQI
Sbjct: 183 LGLEECCLDKAANLESCSIVFAANLYPPCPQPELARGLPSHSDHCLLTILLQNQIAGLQI 242

Query: 241 LHDHNWLNVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNAKATRISIAMAVGPSPET 300
           LH   WLNVNPIPNS LVNVADQLEILSNGKYKSVLHRA+VN KATRISIAMAVGPS ET
Sbjct: 243 LHHDKWLNVNPIPNSLLVNVADQLEILSNGKYKSVLHRAIVNDKATRISIAMAVGPSLET 302

Query: 301 VVGPVEELVHKQANNPPLFKHIKYRDYMEMMQSNQLQGKSSLDRIRL 348
           VVGP  +L++K  NNPPLFK+IKY DYM ++QSN+LQGKS+LDRIRL
Sbjct: 303 VVGPAPQLINKHTNNPPLFKNIKYIDYMGIVQSNKLQGKSTLDRIRL 348

BLAST of Lag0019761 vs. NCBI nr
Match: XP_022141027.1 (protein DMR6-LIKE OXYGENASE 1-like isoform X1 [Momordica charantia] >XP_022159285.1 protein DMR6-LIKE OXYGENASE 1-like isoform X1 [Momordica charantia])

HSP 1 Score: 545.8 bits (1405), Expect = 2.7e-151
Identity = 282/348 (81.03%), Postives = 306/348 (87.93%), Query Frame = 0

Query: 1   MASVKAIAETPNLASIPSSYIFSPDEPDGVAADAAPHGVEVSIPTIDFSLLTDGTPCQRA 60
           +ASVK IAETPNLASIPSSYIFS +  DG AA AAP GVE SIPTIDFSLLT GTP QRA
Sbjct: 6   VASVKTIAETPNLASIPSSYIFSAN--DG-AAKAAPRGVEDSIPTIDFSLLTMGTPDQRA 65

Query: 61  KVVDELGKACQDWGFFMVINHGVEERVMKEMVEICREFFDQKEEEKREYETKDVLDPIRY 120
           KVVDELGKACQDWGFF+V+NHGVEERVM+EM++ICREFFD  EEEK EY+T+ VLDPIRY
Sbjct: 66  KVVDELGKACQDWGFFVVMNHGVEERVMREMIDICREFFDLTEEEKTEYKTEHVLDPIRY 125

Query: 121 GTSFNPKMEKVFFWRDYLKILVHPKFHSPTKPPKFREILEEYCKRIREMSRELVRGISES 180
           GTSFNP+ EKV FWRDYLKI VHPKFHSPTKPP+FREILEEYCKR  EM+RELVRGISES
Sbjct: 126 GTSFNPQKEKVLFWRDYLKIFVHPKFHSPTKPPRFREILEEYCKRSIEMARELVRGISES 185

Query: 181 LGLEGSNLEKAVDLESCSILFAANLYPPCPQPELARGLPSHSDQCLLTLLLQNNIGGLQI 240
           LGLE  +LE+AVD ESCS LFAAN YPP PQPELARGLPSHSDQCLLTLLLQN I GLQI
Sbjct: 186 LGLETCHLERAVDFESCSTLFAANFYPPYPQPELARGLPSHSDQCLLTLLLQNQISGLQI 245

Query: 241 LHDHNWLNVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNAKATRISIAMAVGPSPET 300
           LH  NW++VNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVN KATR+SIA+AVG SPET
Sbjct: 246 LHQGNWVDVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNNKATRLSIAVAVGSSPET 305

Query: 301 VVGPVEELVHKQANNPPLFKHIKYRDYMEMMQSNQLQGKSSLDRIRLL 349
           VV P  +L++K   NPPLFKHIKY DYM+M+QS+ LQ  S LDRIRLL
Sbjct: 306 VVSPAPQLLNK-LTNPPLFKHIKYTDYMQMVQSSNLQ-DSCLDRIRLL 348

BLAST of Lag0019761 vs. ExPASy Swiss-Prot
Match: Q6Z244 (2-oxoglutarate-dependent dioxygenase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD19 PE=1 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 8.5e-76
Identity = 151/335 (45.07%), Postives = 207/335 (61.79%), Query Frame = 0

Query: 16  IPSSYIFSPDEPDGVAADAAPHGVEVSIPTIDFSLLTDGTPCQRAKVVDELGKACQDWGF 75
           +  S + S +E    AA          IP +D  +L +G   +R++ + +LG+AC+DWGF
Sbjct: 2   VAPSRLPSHEEQSAAAAADGSATPSQGIPVVDLGVLINGAADERSRAIRDLGRACEDWGF 61

Query: 76  FMVINHGVEERVMKEMVEICREFFDQKEEEKREYETKDVLDPIRYGTSFNPKMEKVFFWR 135
           FMV NHGV E + + +++ C+E F    EEK+EY     +DPIR GT F   ++ V   R
Sbjct: 62  FMVTNHGVPEALREAIMDACKELFRLPLEEKKEYMRAKPMDPIRIGTGFYSVVDAVPCRR 121

Query: 136 DYLKILVHPKFHSPTKPPKFREILEEYCKRIREMSRELVRGISESLGLEGSNLEKAVDLE 195
           DYLK+  HP+FH P KP K REI  EY    R +  EL + ISESLGL G  L +A++LE
Sbjct: 122 DYLKMFSHPEFHCPEKPAKLREIATEYATCTRALLLELTKAISESLGLAGGRLSEALNLE 181

Query: 196 SCSILFAANLYPPCPQP-ELARGLPSHSDQCLLTLLLQNNIGGLQILHDHNWLNVNPIPN 255
           SC  +   N YP C +P E A GL +HSD  LLTLL QN + GLQ+ HD  WL   P+P 
Sbjct: 182 SCFQILVGNHYPACSRPDEQAMGLSAHSDHGLLTLLFQNGVDGLQVKHDGEWLLAKPLPG 241

Query: 256 SFLVNVADQLEILSNGKYKSVLHRAMVNAKATRISIAMAVGPSPETVVGPVEELVHKQAN 315
           SF V   DQLEI++NG+YK VLHRA+V  + +R+S    +GP  +TVV P+ E+    A+
Sbjct: 242 SFFVIAGDQLEIVTNGRYKGVLHRAVVGGEQSRMSFVSLIGPCMDTVVEPLPEMA---AD 301

Query: 316 NPPL-FKHIKYRDYMEMMQSNQLQGKSSLDRIRLL 349
              L F+ I+YRDYMEM QSN +  K++LD +R++
Sbjct: 302 GRGLEFRGIRYRDYMEMQQSNSINEKTALDIVRVM 333

BLAST of Lag0019761 vs. ExPASy Swiss-Prot
Match: Q6YYX9 (Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japonica OX=39947 GN=SLC1 PE=2 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.5e-61
Identity = 123/302 (40.73%), Postives = 178/302 (58.94%), Query Frame = 0

Query: 13  LASIPSSYIF-SPDEPDGVAADAAPHGVEVSIPTIDFSLLTDGTPCQRAKVVDELGKACQ 72
           +  +P +Y+  + D P   A  AA  G  V +P +D S L    P +R  V+  L  AC+
Sbjct: 49  ITRLPGNYVLPASDRPGQAAGAAAAAGGSVKLPVVDLSRLR--VPSERGAVLRTLDAACR 108

Query: 73  DWGFFMVINHGVEERVMKEMVEICREFFDQKEEEKREYETKDVLDPIRYGTSFNPKMEKV 132
           ++GFF V+NHGV   V+  M+++ R FF+  + E+  Y + DV  P+RYGTSFN   + V
Sbjct: 109 EYGFFQVVNHGVGGEVVGGMLDVARRFFELPQPERERYMSADVRAPVRYGTSFNQVRDAV 168

Query: 133 FFWRDYLKILVHPKF----HSPTKPPKFREILEEYCKRIREMSRELVRGISESLGLEGSN 192
             WRD+LK+   P        PT P   RE+   Y +  + +  E++    E+LG+ G  
Sbjct: 169 LCWRDFLKLACMPLAAVVESWPTSPADLREVASRYAEANQRVFMEVMEAALEALGVGGGG 228

Query: 193 LEKAVDLESCSILFAANLYPPCPQPELARGLPSHSDQCLLTLLLQNNIGGLQILHDHNWL 252
           + +  DL + + +   N YP CPQPEL  G+P HSD   LTL+LQ+ + GLQ++H   WL
Sbjct: 229 VME--DLAAGTQMMTVNCYPECPQPELTLGMPPHSDYGFLTLVLQDEVAGLQVMHAGEWL 288

Query: 253 NVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNAKATRISIAMAVGPSPETVVGPVEE 310
            V+P+P SF+VNV D LEILSNG+Y+SVLHR  VN++  R+S+A     +PE VV P  E
Sbjct: 289 TVDPLPGSFVVNVGDHLEILSNGRYRSVLHRVKVNSRRLRVSVASFHSVAPERVVSPAPE 346

BLAST of Lag0019761 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.0e-60
Identity = 123/309 (39.81%), Postives = 181/309 (58.58%), Query Frame = 0

Query: 42  SIPTIDFSLLTDGTPCQRAKVVDELGKACQDWGFFMVINHGVEERVMKEMVEICREFFDQ 101
           SIP ID   L D     RA ++++   AC   GFF + NHGV E  +K+M+   REFF Q
Sbjct: 41  SIPLID---LHDLHGPNRADIINQFAHACSSCGFFQIKNHGVPEETIKKMMNAAREFFRQ 100

Query: 102 KEEEKREYETKDVLDPIRYGTSFNPKMEKVFFWRDYLKILVHP--KFHS--PTKPPKFRE 161
            E E+ ++ + D     R  TSFN   EKV  WRD+L++  +P   F +  P+ P  FRE
Sbjct: 101 SESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCYPIEDFINEWPSTPISFRE 160

Query: 162 ILEEYCKRIREMSRELVRGISESLGLEGSNLEKAVDLESCSILFAANLYPPCPQPELARG 221
           +  EY   +R +   L+  ISESLGL    +   +      +  A N YP CPQPEL  G
Sbjct: 161 VTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHGQHM--AINYYPRCPQPELTYG 220

Query: 222 LPSHSDQCLLTLLLQNNIGGLQILHDHNWLNVNPIPNSFLVNVADQLEILSNGKYKSVLH 281
           LP H D  L+T+LLQ+ + GLQ+  D  W+ VNP+PN+F+VN+ DQ++++SN KYKSVLH
Sbjct: 221 LPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLH 280

Query: 282 RAMVNAKATRISIAMAVGPSPETVVGPVEELVHKQANNPPLFKHIKYRDYMEMMQSNQLQ 341
           RA+VN+   RISI     PS + V+ P +EL++++ ++P ++++  Y +Y E        
Sbjct: 281 RAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEYFEKFWDTAFD 340

Query: 342 GKSSLDRIR 347
            +S +D  +
Sbjct: 341 TESCIDSFK 344

BLAST of Lag0019761 vs. ExPASy Swiss-Prot
Match: Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 9.5e-59
Identity = 129/347 (37.18%), Postives = 193/347 (55.62%), Query Frame = 0

Query: 4   VKAIAETPNLASIPSSYIFSPDEPDGVAADAAPHGVEVSIPTIDFSLLTDGTPCQRAKVV 63
           V+ I++ PNL+ + SS                      SIP ID   L D     RA +V
Sbjct: 25  VRPISDRPNLSEVESS--------------------GDSIPLID---LRDLHGPNRAVIV 84

Query: 64  DELGKACQDWGFFMVINHGVEERVMKEMVEICREFFDQKEEEKREYETKDVLDPIRYGTS 123
            +L  AC  +GFF + NHGV +  + +M  + REFF Q E E+ ++ + D     R  TS
Sbjct: 85  QQLASACSTYGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTS 144

Query: 124 FNPKMEKVFFWRDYLKILVHP----KFHSPTKPPKFREILEEYCKRIREMSRELVRGISE 183
           FN   +KV  WRD+L++   P        P+ P  FRE+  EY   +R +   L+  ISE
Sbjct: 145 FNVGADKVLNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISE 204

Query: 184 SLGLEGSNLEKAVDLESCSILFAANLYPPCPQPELARGLPSHSDQCLLTLLLQNNIGGLQ 243
           SLGLE  ++   +   +  + F  N YPPCP+PEL  GLP H D  ++T+LLQ+ + GLQ
Sbjct: 205 SLGLESDHISNILGKHAQHMAF--NYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQ 264

Query: 244 ILHDHNWLNVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNAKATRISIAMAVGPSPE 303
           +  D  W+ V+PIPN+F+VN+ DQ++++SN KYKSVLHRA+VN +  R+SI     PS +
Sbjct: 265 VFKDDKWVAVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTD 324

Query: 304 TVVGPVEELVHKQANNPPLFKHIKYRDYMEMMQSNQLQGKSSLDRIR 347
            V+GP  ELV++Q ++  +++   + +Y +   +  L   S LD  +
Sbjct: 325 AVIGPAHELVNEQ-DSLAIYRTYPFVEYWDKFWNRSLATASCLDAFK 345

BLAST of Lag0019761 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 4.0e-57
Identity = 120/312 (38.46%), Postives = 185/312 (59.29%), Query Frame = 0

Query: 40  EVSIPTIDFSLLTDGTPCQRAKVVDELGKACQDWGFFMVINHGVEERVMKEMVEICREFF 99
           +  IP +D +     +P  RA VV  +G AC+  GFF V+NHG++  ++  ++E+ REFF
Sbjct: 40  DARIPVVDLA-----SP-DRAAVVSAVGDACRTHGFFQVVNHGIDAALIASVMEVGREFF 99

Query: 100 DQKEEEKREYETKDVLDPIRYGTSFNPKMEKVFFWRDYLKILVHPKFHS----PTKPPKF 159
               EEK +  + D    IR  TSFN + E V  WRDYL++  +P        P+ PP F
Sbjct: 100 RLPAEEKAKLYSDDPAKKIRLSTSFNVRKETVHNWRDYLRLHCYPLHQFVPDWPSNPPSF 159

Query: 160 REILEEYCKRIREMSRELVRGISESLGLEGSNLEKAVDLESCSILFAANLYPPCPQPELA 219
           +EI+  YC  +RE+   L   ISESLGLEG  + + +  +   +  A N YP CP+PEL 
Sbjct: 160 KEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGEQEQHM--AVNYYPQCPEPELT 219

Query: 220 RGLPSHSDQCLLTLLLQNN-IGGLQILHDHNWLNVNPIPNSFLVNVADQLEILSNGKYKS 279
            GLP+H+D   LT+LL ++ + GLQ+L+D  W+ VNP P + ++N+ DQL+ LSNGKY+S
Sbjct: 220 YGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALVINIGDQLQALSNGKYRS 279

Query: 280 VLHRAMVNAKATRISIAMAVGPSPETVVGPVEELVHKQANNPPLFKHIKYRDYMEMMQSN 339
           V HRA+VN+   R+S+A  + P     +GP ++L+    ++P ++++  Y +Y +   S 
Sbjct: 280 VWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLI--TDDSPAVYRNYTYDEYYKKFWSR 339

Query: 340 QLQGKSSLDRIR 347
            L  +  L+  R
Sbjct: 340 NLDQEHCLELFR 341

BLAST of Lag0019761 vs. ExPASy TrEMBL
Match: A0A6J1JCE3 (protein DMR6-LIKE OXYGENASE 2-like OS=Cucurbita maxima OX=3661 GN=LOC111483195 PE=3 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 3.9e-156
Identity = 283/351 (80.63%), Postives = 311/351 (88.60%), Query Frame = 0

Query: 1   MASVKAIAETPNLASIPSSYIF-SPDEPDGV--AADAAPHGVEVSIPTIDFSLLTDGTPC 60
           + SVKAIAETPNLASIPSSYIF + D+ D V  AADAAPH VEVSIPTIDFSLLT GT  
Sbjct: 34  LPSVKAIAETPNLASIPSSYIFTTSDDSDDVAAAADAAPHRVEVSIPTIDFSLLTTGTSH 93

Query: 61  QRAKVVDELGKACQDWGFFMVINHGVEERVMKEMVEICREFFDQKEEEKREYETKDVLDP 120
           QR+KVV+ELGKAC DWGFFMVINHGV E +MKEMVEICREFFD KEEEKREYETK VLDP
Sbjct: 94  QRSKVVNELGKACHDWGFFMVINHGVGEGLMKEMVEICREFFDLKEEEKREYETKHVLDP 153

Query: 121 IRYGTSFNPKMEKVFFWRDYLKILVHPKFHSPTKPPKFREILEEYCKRIREMSRELVRGI 180
           IRYGTSFNPKME+VFFWRDYLK+LVHPKFHSP KP +FREILEEY KRIREM+RELVRGI
Sbjct: 154 IRYGTSFNPKMEEVFFWRDYLKVLVHPKFHSPPKPTRFREILEEYSKRIREMARELVRGI 213

Query: 181 SESLGLEGSNLEKAVDLESCSILFAANLYPPCPQPELARGLPSHSDQCLLTLLLQNNIGG 240
           SESLGLE  NLEKA +LES S LFAANLYPPCPQP+ ARGLP HSDQCLLT+LLQN++ G
Sbjct: 214 SESLGLEAYNLEKAAELESSSTLFAANLYPPCPQPQFARGLPPHSDQCLLTVLLQNHVSG 273

Query: 241 LQILHDHNWLNVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNAKATRISIAMAVGPS 300
           LQILHDH WL VNP+PN+ LVN ADQLEI+SNGKYKSVLHRAMVN KATRISIAMA+GPS
Sbjct: 274 LQILHDHQWLTVNPVPNALLVNTADQLEIMSNGKYKSVLHRAMVNDKATRISIAMAIGPS 333

Query: 301 PETVVGPVEELVHKQANNPPLFKHIKYRDYMEMMQSNQLQGKSSLDRIRLL 349
            +T+V P+ EL+HK  NNPPLFK IKY+DYMEM+QSN+L+ KS LDR+RLL
Sbjct: 334 SQTLVAPLPELLHKH-NNPPLFKTIKYKDYMEMLQSNKLERKSCLDRLRLL 383

BLAST of Lag0019761 vs. ExPASy TrEMBL
Match: A0A6J1CIQ0 (protein DMR6-LIKE OXYGENASE 1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011533 PE=3 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.3e-151
Identity = 282/348 (81.03%), Postives = 306/348 (87.93%), Query Frame = 0

Query: 1   MASVKAIAETPNLASIPSSYIFSPDEPDGVAADAAPHGVEVSIPTIDFSLLTDGTPCQRA 60
           +ASVK IAETPNLASIPSSYIFS +  DG AA AAP GVE SIPTIDFSLLT GTP QRA
Sbjct: 6   VASVKTIAETPNLASIPSSYIFSAN--DG-AAKAAPRGVEDSIPTIDFSLLTMGTPDQRA 65

Query: 61  KVVDELGKACQDWGFFMVINHGVEERVMKEMVEICREFFDQKEEEKREYETKDVLDPIRY 120
           KVVDELGKACQDWGFF+V+NHGVEERVM+EM++ICREFFD  EEEK EY+T+ VLDPIRY
Sbjct: 66  KVVDELGKACQDWGFFVVMNHGVEERVMREMIDICREFFDLTEEEKTEYKTEHVLDPIRY 125

Query: 121 GTSFNPKMEKVFFWRDYLKILVHPKFHSPTKPPKFREILEEYCKRIREMSRELVRGISES 180
           GTSFNP+ EKV FWRDYLKI VHPKFHSPTKPP+FREILEEYCKR  EM+RELVRGISES
Sbjct: 126 GTSFNPQKEKVLFWRDYLKIFVHPKFHSPTKPPRFREILEEYCKRSIEMARELVRGISES 185

Query: 181 LGLEGSNLEKAVDLESCSILFAANLYPPCPQPELARGLPSHSDQCLLTLLLQNNIGGLQI 240
           LGLE  +LE+AVD ESCS LFAAN YPP PQPELARGLPSHSDQCLLTLLLQN I GLQI
Sbjct: 186 LGLETCHLERAVDFESCSTLFAANFYPPYPQPELARGLPSHSDQCLLTLLLQNQISGLQI 245

Query: 241 LHDHNWLNVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNAKATRISIAMAVGPSPET 300
           LH  NW++VNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVN KATR+SIA+AVG SPET
Sbjct: 246 LHQGNWVDVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNNKATRLSIAVAVGSSPET 305

Query: 301 VVGPVEELVHKQANNPPLFKHIKYRDYMEMMQSNQLQGKSSLDRIRLL 349
           VV P  +L++K   NPPLFKHIKY DYM+M+QS+ LQ  S LDRIRLL
Sbjct: 306 VVSPAPQLLNK-LTNPPLFKHIKYTDYMQMVQSSNLQ-DSCLDRIRLL 348

BLAST of Lag0019761 vs. ExPASy TrEMBL
Match: A0A1S3CJI6 (protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo OX=3656 GN=LOC103501162 PE=3 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 6.4e-151
Identity = 269/348 (77.30%), Postives = 300/348 (86.21%), Query Frame = 0

Query: 1   MASVKAIAETPNLASIPSSYIFSPDEP-DGVAADAAPHGVEVSIPTIDFSLLTDGTPCQR 60
           MASVK+IA+TPNL SIPSS+IF+ D+  D V ADA+P G E SIP ID SLL +GTP QR
Sbjct: 1   MASVKSIADTPNLTSIPSSFIFATDDSFDDVTADASPQGAEDSIPIIDLSLLINGTPQQR 60

Query: 61  AKVVDELGKACQDWGFFMVINHGVEERVMKEMVEICREFFDQKEEEKREYETKDVLDPIR 120
           AKVV+ELGKAC+DWGFFMV+NHGVEE++MK+++EIC EFF+ KEEEKREYETK VLDPIR
Sbjct: 61  AKVVNELGKACEDWGFFMVVNHGVEEKLMKDLMEICIEFFELKEEEKREYETKHVLDPIR 120

Query: 121 YGTSFNPKMEKVFFWRDYLKILVHPKFHSPTKPPKFREILEEYCKRIREMSRELVRGISE 180
           YGTSFNPK+EK FFWRDYLKI VHPKFH+PTKP +FR ILEEYC R+RE +RELVRGISE
Sbjct: 121 YGTSFNPKVEKAFFWRDYLKIKVHPKFHAPTKPTRFRGILEEYCTRVRETTRELVRGISE 180

Query: 181 SLGLEGSNLEKAVDLESCSILFAANLYPPCPQPELARGLPSHSDQCLLTLLLQNNIGGLQ 240
           SLGLEG  LEKA DLES  ILFAANLYPPCPQPELARGLPSHSD CLLT+L+ N I GLQ
Sbjct: 181 SLGLEGCFLEKATDLESSLILFAANLYPPCPQPELARGLPSHSDLCLLTILITNEIAGLQ 240

Query: 241 ILHDHNWLNVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNAKATRISIAMAVGPSPE 300
           ILH   W NVNPIPNS ++NV DQLEILSNGKYKSVLHRA VN KATRISI MAVGPS E
Sbjct: 241 ILHHDKWFNVNPIPNSLIINVGDQLEILSNGKYKSVLHRAKVNDKATRISIGMAVGPSHE 300

Query: 301 TVVGPVEELVHKQANNPPLFKHIKYRDYMEMMQSNQLQGKSSLDRIRL 348
           TVVGP  +LV++  NNPP+FK IKY+DYME+MQSNQLQGKS LDR RL
Sbjct: 301 TVVGPAPQLVNEHTNNPPMFKSIKYKDYMEIMQSNQLQGKSILDRFRL 348

BLAST of Lag0019761 vs. ExPASy TrEMBL
Match: A0A5A7UYU4 (Protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold753G00070 PE=3 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 1.9e-150
Identity = 268/348 (77.01%), Postives = 299/348 (85.92%), Query Frame = 0

Query: 1   MASVKAIAETPNLASIPSSYIFSPDEP-DGVAADAAPHGVEVSIPTIDFSLLTDGTPCQR 60
           MASVK+IA+TPNL SIPSS+IF+ D+  D V ADA+P G E SIP ID SLL +GTP QR
Sbjct: 1   MASVKSIADTPNLTSIPSSFIFATDDSFDDVTADASPQGAEDSIPIIDLSLLINGTPQQR 60

Query: 61  AKVVDELGKACQDWGFFMVINHGVEERVMKEMVEICREFFDQKEEEKREYETKDVLDPIR 120
           AKVV+ELGKAC+DWGFFMV+NHGVEE++MKE++EIC EFF+ KEEEKREYETK VLDPIR
Sbjct: 61  AKVVNELGKACEDWGFFMVVNHGVEEKLMKELMEICIEFFELKEEEKREYETKHVLDPIR 120

Query: 121 YGTSFNPKMEKVFFWRDYLKILVHPKFHSPTKPPKFREILEEYCKRIREMSRELVRGISE 180
           YGTSFNPK+EK FFWRDYLKI VHPKFH+PTKP + R ILEEYC R+RE +RELVRGISE
Sbjct: 121 YGTSFNPKVEKAFFWRDYLKIKVHPKFHAPTKPTRLRGILEEYCTRVRETTRELVRGISE 180

Query: 181 SLGLEGSNLEKAVDLESCSILFAANLYPPCPQPELARGLPSHSDQCLLTLLLQNNIGGLQ 240
           SLGLEG  LEKA DLES  ILFAANLYPPCPQPELARGLPSHSD CLLT+L+ N I GLQ
Sbjct: 181 SLGLEGCFLEKATDLESSLILFAANLYPPCPQPELARGLPSHSDLCLLTILITNEIAGLQ 240

Query: 241 ILHDHNWLNVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNAKATRISIAMAVGPSPE 300
           ILH   W NVNPIPNS ++NV DQLEILSNGKYKSVLHRA VN KATRISI MAVGPS E
Sbjct: 241 ILHHDKWFNVNPIPNSLIINVGDQLEILSNGKYKSVLHRAKVNDKATRISIGMAVGPSHE 300

Query: 301 TVVGPVEELVHKQANNPPLFKHIKYRDYMEMMQSNQLQGKSSLDRIRL 348
           TVVGP  +L+++  NNPP+FK IKY+DYME+MQSNQLQGKS LDR RL
Sbjct: 301 TVVGPAPQLINEHTNNPPMFKSIKYKDYMEIMQSNQLQGKSILDRFRL 348

BLAST of Lag0019761 vs. ExPASy TrEMBL
Match: A0A0A0L0R1 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G112650 PE=3 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 2.7e-149
Identity = 267/348 (76.72%), Postives = 301/348 (86.49%), Query Frame = 0

Query: 1   MASVKAIAETPNLASIPSSYIFSPDEP-DGVAADAAPHGVEVSIPTIDFSLLTDGTPCQR 60
           MASVK+IA++PNL SIPSS+IF+ D+  D VAADA+  G E SIP ID SLL +GTP QR
Sbjct: 1   MASVKSIADSPNLTSIPSSFIFATDDSFDDVAADASLQGAEDSIPIIDLSLLINGTPQQR 60

Query: 61  AKVVDELGKACQDWGFFMVINHGVEERVMKEMVEICREFFDQKEEEKREYETKDVLDPIR 120
           AKVV+ELGKAC+DWGFFMV+NHGVEE++MK+++EIC EFF+ KEEEKREYETK VLDPIR
Sbjct: 61  AKVVNELGKACEDWGFFMVVNHGVEEKLMKDLMEICVEFFELKEEEKREYETKHVLDPIR 120

Query: 121 YGTSFNPKMEKVFFWRDYLKILVHPKFHSPTKPPKFREILEEYCKRIREMSRELVRGISE 180
           YGTSFNPKMEK FFWRDYLKI+VHPKFH+PTKP +FR ILEEYC  +REM+REL+RGISE
Sbjct: 121 YGTSFNPKMEKAFFWRDYLKIMVHPKFHAPTKPTRFRGILEEYCTSVREMTRELLRGISE 180

Query: 181 SLGLEGSNLEKAVDLESCSILFAANLYPPCPQPELARGLPSHSDQCLLTLLLQNNIGGLQ 240
           SLGLEG  LEKA DLES  ILFAANLYPPCPQPELARGLPSHSD CLLT+LL N I GLQ
Sbjct: 181 SLGLEGCFLEKATDLESSLILFAANLYPPCPQPELARGLPSHSDLCLLTILLTNQIAGLQ 240

Query: 241 ILHDHNWLNVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNAKATRISIAMAVGPSPE 300
           ILH   W NVNPIPNSF++NV DQLEILSNGKY+SVLHRA VN KATRISI MAVGPS E
Sbjct: 241 ILHHDKWFNVNPIPNSFIINVGDQLEILSNGKYESVLHRAKVNDKATRISIGMAVGPSHE 300

Query: 301 TVVGPVEELVHKQANNPPLFKHIKYRDYMEMMQSNQLQGKSSLDRIRL 348
           TVVGP  +LV++  NNPP+FK IKY+DYME+MQS+QLQ KS LDR RL
Sbjct: 301 TVVGPAPQLVNEDTNNPPMFKSIKYKDYMEIMQSSQLQEKSILDRFRL 348

BLAST of Lag0019761 vs. TAIR 10
Match: AT2G36690.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 250.4 bits (638), Expect = 2.2e-66
Identity = 142/346 (41.04%), Postives = 204/346 (58.96%), Query Frame = 0

Query: 4   VKAIAETPNLASIPSSYIF-SPDEPDGVAAD-AAPHGVEVSIPTIDFSLLTDGTPCQRAK 63
           VK + E   L  +P+ YI+  PD P    +D        + +P IDF+ L       R  
Sbjct: 21  VKHLCEN-GLTKVPTKYIWPEPDRPILTKSDKLIKPNKNLKLPLIDFAELLGP---NRPH 80

Query: 64  VVDELGKACQDWGFFMVINHGVEERVMKEMVEICREFFDQKEEEKREYETKDVLDPIRYG 123
           V+  + +AC+ +GFF V+NHG+E  V K M+++C+ FF+   EE+ +Y + D+  P+RYG
Sbjct: 81  VLRTIAEACKTYGFFQVVNHGMEGDVSKNMIDVCKRFFELPYEERSKYMSSDMSAPVRYG 140

Query: 124 TSFNPKMEKVFFWRDYLKILVHP----KFHSPTKPPKFREILEEYCKRIREMSRELVRGI 183
           TSFN   + VF WRD+LK+  HP      H P+ P  FR     Y K  +EM   +V+ I
Sbjct: 141 TSFNQIKDNVFCWRDFLKLYAHPLPDYLPHWPSSPSDFRSSAATYAKETKEMFEMMVKAI 200

Query: 184 SESLGLEGSNLEKAVDLESCSILFAANLYPPCPQPELARGLPSHSDQCLLTLLLQNNIGG 243
            ESL ++GS+ E A +LE  S +   N YPPCP+PEL  G+P HSD   LTLLLQ+ + G
Sbjct: 201 LESLEIDGSD-EAAKELEEGSQVVVVNCYPPCPEPELTLGMPPHSDYGFLTLLLQDEVEG 260

Query: 244 LQILHDHNWLNVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNAKATRISIAMAVGPS 303
           LQIL+   W+ V+PIP SF+VNV D LEI SNG+YKSVLHR +VN+   RIS+A      
Sbjct: 261 LQILYRDEWVTVDPIPGSFVVNVGDHLEIFSNGRYKSVLHRVLVNSTKPRISVASLHSFP 320

Query: 304 PETVVGPVEELVHKQANNPPLFKHIKYRDYMEMMQSNQLQGKSSLD 344
             +VV P  +LV K  +NP  +    +  +++ + S + + K+ L+
Sbjct: 321 LTSVVKPSPKLVDK--HNPSQYMDTDFTTFLQYITSREPKWKNFLE 359

BLAST of Lag0019761 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 235.3 bits (599), Expect = 7.2e-62
Identity = 123/309 (39.81%), Postives = 181/309 (58.58%), Query Frame = 0

Query: 42  SIPTIDFSLLTDGTPCQRAKVVDELGKACQDWGFFMVINHGVEERVMKEMVEICREFFDQ 101
           SIP ID   L D     RA ++++   AC   GFF + NHGV E  +K+M+   REFF Q
Sbjct: 41  SIPLID---LHDLHGPNRADIINQFAHACSSCGFFQIKNHGVPEETIKKMMNAAREFFRQ 100

Query: 102 KEEEKREYETKDVLDPIRYGTSFNPKMEKVFFWRDYLKILVHP--KFHS--PTKPPKFRE 161
            E E+ ++ + D     R  TSFN   EKV  WRD+L++  +P   F +  P+ P  FRE
Sbjct: 101 SESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCYPIEDFINEWPSTPISFRE 160

Query: 162 ILEEYCKRIREMSRELVRGISESLGLEGSNLEKAVDLESCSILFAANLYPPCPQPELARG 221
           +  EY   +R +   L+  ISESLGL    +   +      +  A N YP CPQPEL  G
Sbjct: 161 VTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHGQHM--AINYYPRCPQPELTYG 220

Query: 222 LPSHSDQCLLTLLLQNNIGGLQILHDHNWLNVNPIPNSFLVNVADQLEILSNGKYKSVLH 281
           LP H D  L+T+LLQ+ + GLQ+  D  W+ VNP+PN+F+VN+ DQ++++SN KYKSVLH
Sbjct: 221 LPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLH 280

Query: 282 RAMVNAKATRISIAMAVGPSPETVVGPVEELVHKQANNPPLFKHIKYRDYMEMMQSNQLQ 341
           RA+VN+   RISI     PS + V+ P +EL++++ ++P ++++  Y +Y E        
Sbjct: 281 RAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEYFEKFWDTAFD 340

Query: 342 GKSSLDRIR 347
            +S +D  +
Sbjct: 341 TESCIDSFK 344

BLAST of Lag0019761 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 228.8 bits (582), Expect = 6.7e-60
Identity = 129/347 (37.18%), Postives = 193/347 (55.62%), Query Frame = 0

Query: 4   VKAIAETPNLASIPSSYIFSPDEPDGVAADAAPHGVEVSIPTIDFSLLTDGTPCQRAKVV 63
           V+ I++ PNL+ + SS                      SIP ID   L D     RA +V
Sbjct: 25  VRPISDRPNLSEVESS--------------------GDSIPLID---LRDLHGPNRAVIV 84

Query: 64  DELGKACQDWGFFMVINHGVEERVMKEMVEICREFFDQKEEEKREYETKDVLDPIRYGTS 123
            +L  AC  +GFF + NHGV +  + +M  + REFF Q E E+ ++ + D     R  TS
Sbjct: 85  QQLASACSTYGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTS 144

Query: 124 FNPKMEKVFFWRDYLKILVHP----KFHSPTKPPKFREILEEYCKRIREMSRELVRGISE 183
           FN   +KV  WRD+L++   P        P+ P  FRE+  EY   +R +   L+  ISE
Sbjct: 145 FNVGADKVLNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISE 204

Query: 184 SLGLEGSNLEKAVDLESCSILFAANLYPPCPQPELARGLPSHSDQCLLTLLLQNNIGGLQ 243
           SLGLE  ++   +   +  + F  N YPPCP+PEL  GLP H D  ++T+LLQ+ + GLQ
Sbjct: 205 SLGLESDHISNILGKHAQHMAF--NYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQ 264

Query: 244 ILHDHNWLNVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNAKATRISIAMAVGPSPE 303
           +  D  W+ V+PIPN+F+VN+ DQ++++SN KYKSVLHRA+VN +  R+SI     PS +
Sbjct: 265 VFKDDKWVAVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTD 324

Query: 304 TVVGPVEELVHKQANNPPLFKHIKYRDYMEMMQSNQLQGKSSLDRIR 347
            V+GP  ELV++Q ++  +++   + +Y +   +  L   S LD  +
Sbjct: 325 AVIGPAHELVNEQ-DSLAIYRTYPFVEYWDKFWNRSLATASCLDAFK 345

BLAST of Lag0019761 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 221.1 bits (562), Expect = 1.4e-57
Identity = 122/303 (40.26%), Postives = 186/303 (61.39%), Query Frame = 0

Query: 47  DFSLLTDGTPCQRAKVVDELGKACQDWGFFMVINHGVEERVMKEMVEICREFFDQKEEEK 106
           DF L+ D +   R+ ++ ++ +AC  +GFF VINHGV ++++ EMV + REFF    EEK
Sbjct: 37  DFPLI-DLSSTDRSFLIQQIHQACARFGFFQVINHGVNKQIIDEMVSVAREFFSMSMEEK 96

Query: 107 REYETKDVLDPIRYGTSFNPKMEKVFFWRDYLKILVHP--KFHS--PTKPPKFREILEEY 166
            +  + D     R  TSFN K E+V  WRDYL++  +P  K+ +  P+ PP F+EI+ +Y
Sbjct: 97  MKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKY 156

Query: 167 CKRIREMSRELVRGISESLGLEGSNLEKAVDLESCSILFAANLYPPCPQPELARGLPSHS 226
            + +RE+  ++   ISESLGLE   ++K +  +   +  A N YPPCP+PEL  GLP+H+
Sbjct: 157 SREVREVGFKIEELISESLGLEKDYMKKVLGEQGQHM--AVNYYPPCPEPELTYGLPAHT 216

Query: 227 DQCLLTLLLQN-NIGGLQILHDHNWLNVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMV 286
           D   LT+LLQ+  + GLQIL D  W  VNP P++F++N+ DQL+ LSNG YKSV HRA+ 
Sbjct: 217 DPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFVINIGDQLQALSNGVYKSVWHRAVT 276

Query: 287 NAKATRISIAMAVGPSPETVVGPVEELVHKQAN-NPPLFKHIKYRDYMEMMQSNQLQGKS 344
           N +  R+S+A  + P+   V+ P + L   + +   P++K   Y +Y +   S  L  + 
Sbjct: 277 NTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETKPVYKDFTYAEYYKKFWSRNLDQEH 336

BLAST of Lag0019761 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 211.1 bits (536), Expect = 1.5e-54
Identity = 131/352 (37.22%), Postives = 192/352 (54.55%), Query Frame = 0

Query: 7   IAETPNLASIPSSYIFSPDEPDGVAADAAPHGVEVSIPTIDFSLLTDGTPCQRAKVVDEL 66
           I   PN   +P  +I    E   V +    H +   IP ID S L+          + +L
Sbjct: 21  IKSKPN--KVPERFIREEYERGVVVSSLKTHHLHHQIPVIDLSKLSKPDNDDFFFEILKL 80

Query: 67  GKACQDWGFFMVINHGVEERVMKEMVEICREFFDQKEEEKREYETKDVLDP---IRYGTS 126
            +AC+DWGFF VINHG+E  V++++ E+  EFFD   EEK++Y     ++P     YG +
Sbjct: 81  SQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMPLEEKKKYP----MEPGTVQGYGQA 140

Query: 127 FNPKMEKVFFWRDYLKILVHPKFHS-----PTKPPKFREILEEYCKRIREMSRELVRGIS 186
           F    ++   W +   + VHP         P+KP +F E LE Y K IRE+ + L++ I+
Sbjct: 141 FIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSESLEGYSKEIRELCKRLLKYIA 200

Query: 187 ESLGLEGSNLEKAVDLESCSILFAANLYPPCPQPELARGLPSHSDQCLLTLLLQ--NNIG 246
            SLGL+    E+       ++    N YPPC  P+L  GL  HSD   LT+L Q  N+  
Sbjct: 201 ISLGLKEERFEEMFGEAVQAV--RMNYYPPCSSPDLVLGLSPHSDGSALTVLQQSKNSCV 260

Query: 247 GLQILHDHNWLNVNPIPNSFLVNVADQLEILSNGKYKSVLHRAMVNAKATRISIAMAVGP 306
           GLQIL D+ W+ V P+PN+ ++N+ D +E+LSNGKYKSV HRA+ N +  R++I     P
Sbjct: 261 GLQILKDNTWVPVKPLPNALVINIGDTIEVLSNGKYKSVEHRAVTNREKERLTIVTFYAP 320

Query: 307 SPETVVGPVEELVHKQANNPPLFKHIKYRDYMEMMQSNQLQGKSSLDRIRLL 349
           + E  + P+ ELV  +  NP  ++   + DY     SN+LQGK SLD  ++L
Sbjct: 321 NYEVEIEPMSELVDDE-TNPCKYRSYNHGDYSYHYVSNKLQGKKSLDFAKIL 363

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022985114.18.0e-15680.63protein DMR6-LIKE OXYGENASE 2-like [Cucurbita maxima][more]
KAG6576847.11.0e-15580.342-oxoglutarate-dependent dioxygenase 19, partial [Cucurbita argyrosperma subsp. ... [more]
XP_023552481.15.2e-15580.34protein DMR6-LIKE OXYGENASE 2-like [Cucurbita pepo subsp. pepo][more]
XP_038904929.12.6e-15479.25protein DMR6-LIKE OXYGENASE 2-like [Benincasa hispida][more]
XP_022141027.12.7e-15181.03protein DMR6-LIKE OXYGENASE 1-like isoform X1 [Momordica charantia] >XP_02215928... [more]
Match NameE-valueIdentityDescription
Q6Z2448.5e-7645.072-oxoglutarate-dependent dioxygenase 19 OS=Oryza sativa subsp. japonica OX=39947... [more]
Q6YYX93.5e-6140.73Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japoni... [more]
Q9ZSA71.0e-6039.81Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1[more]
Q9ZSA89.5e-5937.18Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1[more]
Q8W2X54.0e-5738.46Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1JCE33.9e-15680.63protein DMR6-LIKE OXYGENASE 2-like OS=Cucurbita maxima OX=3661 GN=LOC111483195 P... [more]
A0A6J1CIQ01.3e-15181.03protein DMR6-LIKE OXYGENASE 1-like isoform X1 OS=Momordica charantia OX=3673 GN=... [more]
A0A1S3CJI66.4e-15177.30protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo OX=3656 GN=LOC103501162 PE=3 ... [more]
A0A5A7UYU41.9e-15077.01Protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A0A0L0R12.7e-14976.72Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G... [more]
Match NameE-valueIdentityDescription
AT2G36690.12.2e-6641.042-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G10490.17.2e-6239.812-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G10500.16.7e-6037.182-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G24530.11.4e-5740.262-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G21420.11.5e-5437.222-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 1..337
e-value: 4.3E-97
score: 327.4
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 201..292
e-value: 1.2E-24
score: 86.7
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 43..148
e-value: 1.2E-24
score: 87.4
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 3..342
NoneNo IPR availablePANTHERPTHR47990:SF47FLAVONOL SYNTHASE/FLAVANONE 3-HYDROXYLASEcoord: 3..342
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 12..339
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 193..296
score: 12.571028

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0019761.1Lag0019761.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity