Lag0019754 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0019754
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	proline-rich receptor-like protein kinase PERK2
Location	chr5: 45137682 .. 45138080 (-)
RNA-Seq Expression	Lag0019754
Synteny	Lag0019754

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATCTTCCAAGTACTTGATCATAGCTTTCTTCATGGCTACTTTGGTGTTTTCGACCATCGACACCGGTCGAGCCGCTCGTCATCTCTTGCAAACCGCTCCACCAAACTTGCCGGGGTTGCCCTCGTTGCCTAAACCGACTGCGCTGCCACCTCTACCGCCCTTGCCTACGATGCCAACGACCCCGACGTCGGGGCCGGGAATTCCCTCTATCCCAAACCTCCCACAACCAACATTGCCAACTACTCAACCATCTTTGCCTAAGTTACCTCCATTCCCAAGCTTCCCCGGTATACCGACGGGAATGGCGCTGCCTCCACTCCCGACGAATTTGCTTCCGAACTTACCGTCGATCCCTTTTCTCACTCCGCCACCGGCCACCACCTCTTCACCTTGA

mRNA sequence

ATGGCATCTTCCAAGTACTTGATCATAGCTTTCTTCATGGCTACTTTGGTGTTTTCGACCATCGACACCGGTCGAGCCGCTCGTCATCTCTTGCAAACCGCTCCACCAAACTTGCCGGGGTTGCCCTCGTTGCCTAAACCGACTGCGCTGCCACCTCTACCGCCCTTGCCTACGATGCCAACGACCCCGACGTCGGGGCCGGGAATTCCCTCTATCCCAAACCTCCCACAACCAACATTGCCAACTACTCAACCATCTTTGCCTAAGTTACCTCCATTCCCAAGCTTCCCCGGTATACCGACGGGAATGGCGCTGCCTCCACTCCCGACGAATTTGCTTCCGAACTTACCGTCGATCCCTTTTCTCACTCCGCCACCGGCCACCACCTCTTCACCTTGA

Coding sequence (CDS)

ATGGCATCTTCCAAGTACTTGATCATAGCTTTCTTCATGGCTACTTTGGTGTTTTCGACCATCGACACCGGTCGAGCCGCTCGTCATCTCTTGCAAACCGCTCCACCAAACTTGCCGGGGTTGCCCTCGTTGCCTAAACCGACTGCGCTGCCACCTCTACCGCCCTTGCCTACGATGCCAACGACCCCGACGTCGGGGCCGGGAATTCCCTCTATCCCAAACCTCCCACAACCAACATTGCCAACTACTCAACCATCTTTGCCTAAGTTACCTCCATTCCCAAGCTTCCCCGGTATACCGACGGGAATGGCGCTGCCTCCACTCCCGACGAATTTGCTTCCGAACTTACCGTCGATCCCTTTTCTCACTCCGCCACCGGCCACCACCTCTTCACCTTGA

Protein sequence

MASSKYLIIAFFMATLVFSTIDTGRAARHLLQTAPPNLPGLPSLPKPTALPPLPPLPTMPTTPTSGPGIPSIPNLPQPTLPTTQPSLPKLPPFPSFPGIPTGMALPPLPTNLLPNLPSIPFLTPPPATTSSP

Homology

[Close]

BLAST of Lag0019754 vs. NCBI nr
Match: XP_023518060.1 (proline-rich receptor-like protein kinase PERK2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 183.7 bits (465), Expect = 1.0e-42
Identity = 110/133 (82.71%), Postives = 116/133 (87.22%), Query Frame = 0


Query: 1   MASSKYLIIAFFMATLVFSTIDTGRAARHLLQTAPPNLPGLPSLPKPTALPPLPPLPTMP 60
           MAS KYLIIA F+ATLVFS IDTGRAARHLLQTAPPN+PGLPS      LPPLPPLPTMP
Sbjct: 1   MASFKYLIIA-FVATLVFSAIDTGRAARHLLQTAPPNVPGLPS------LPPLPPLPTMP 60

Query: 61  TTPTSGPGIPSIPNLPQPTLPTTQPSLPKLPPFPSFP-GIPTGMALPPLPTNLLPNLPSI 120
             PT+G GIPSIP+LPQPTLPTTQPSLPKLPPFP+FP  IPTGMALPPLPT LLPNLPSI
Sbjct: 61  -NPTTGSGIPSIPSLPQPTLPTTQPSLPKLPPFPTFPSNIPTGMALPPLPTTLLPNLPSI 120

Query: 121 PFLTPPPATTSSP 133
           P LTPPP T+SSP
Sbjct: 121 PSLTPPPTTSSSP 125

[Close]

BLAST of Lag0019754 vs. NCBI nr
Match: XP_023518061.1 (protein PELPK1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 179.9 bits (455), Expect = 1.5e-41
Identity = 111/136 (81.62%), Postives = 115/136 (84.56%), Query Frame = 0


Query: 1   MASSKYLIIAFFMATLVFSTIDTGRAARHLLQTAPPNLPGLPSLPKPTALPPLPPLPTMP 60
           MASSKYLIIA FM TL+FS IDTGRAARHLLQTAPPN+P LPS      LPPLPPLPTMP
Sbjct: 1   MASSKYLIIA-FMVTLMFSAIDTGRAARHLLQTAPPNVPRLPS------LPPLPPLPTMP 60

Query: 61  TTPTSGPGIPSIPNLPQ---PTLPTTQPSLPKLPPFPSFP-GIPTGMALPPLPTNLLPNL 120
             PT+GPGIPSIP+LPQ   PTLPTTQPSLPKLPP PSFP  IPTGMALPPLPT LLPNL
Sbjct: 61  -NPTTGPGIPSIPSLPQPTLPTLPTTQPSLPKLPPLPSFPRNIPTGMALPPLPTTLLPNL 120

Query: 121 PSIPFLTPPPATTSSP 133
           PSIP LTPPP TTSSP
Sbjct: 121 PSIPSLTPPPTTTSSP 128

[Close]

BLAST of Lag0019754 vs. NCBI nr
Match: KAG6595524.1 (hypothetical protein SDJN03_12077, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 174.5 bits (441), Expect = 6.1e-40
Identity = 106/132 (80.30%), Postives = 113/132 (85.61%), Query Frame = 0


Query: 1   MASSKYLIIAFFMATLVFSTIDTGRAARHLLQTAPPNLPGLPSLPKPTALPPLPPLPTMP 60
           MASSKYLIIA FMATL+FS I+TGRAARHLLQTAPPN+PGLPS      LPPLPPLPTMP
Sbjct: 1   MASSKYLIIA-FMATLMFSAINTGRAARHLLQTAPPNVPGLPS------LPPLPPLPTMP 60

Query: 61  TTPTSGPGIPSIPNLPQPTLPTTQPSLPKLPPFPSFPGIPTGMALPPLPTNLLPNLPSIP 120
             PT+GPGIPSIP+LPQPTLPTTQPSLPKL P      +PTGMALPPLPT LLPNLPSIP
Sbjct: 61  -NPTTGPGIPSIPSLPQPTLPTTQPSLPKLSP------LPTGMALPPLPTTLLPNLPSIP 118

Query: 121 FLTPPPATTSSP 133
            LTPPP+TTSSP
Sbjct: 121 SLTPPPSTTSSP 118

[Close]

BLAST of Lag0019754 vs. NCBI nr
Match: XP_038904304.1 (RNA-binding protein 12-like [Benincasa hispida])

HSP 1 Score: 173.3 bits (438), Expect = 1.4e-39
Identity = 108/141 (76.60%), Postives = 113/141 (80.14%), Query Frame = 0


Query: 1   MASSKYLIIAFFMATLVFSTIDTGRAARHLLQTAPPNLPGLPSLPKPTALPPLPPLPTMP 60
           MASSKYL+I  FMA LVFSTIDTGRAARHLLQTAPPN+PG PSLPKPTALPPLPPLPT  
Sbjct: 1   MASSKYLMIIAFMAILVFSTIDTGRAARHLLQTAPPNMPGFPSLPKPTALPPLPPLPT-- 60

Query: 61  TTPTSGPGIPSIPNLPQPTLPTTQPSLPKLPPFPSFPG-IP-TGMALPPLPTNLLPNL-- 120
                GPG+PSIPNLPQPTLP+   SLPKLPP PSFPG IP TG+ LPPLPT  LP L  
Sbjct: 61  -----GPGMPSIPNLPQPTLPS---SLPKLPPLPSFPGNIPVTGVTLPPLPTTSLPTLPS 120

Query: 121 -----PSIPFLTPPPATTSSP 133
                PSIPFLTPPPATTSSP
Sbjct: 121 IPTTFPSIPFLTPPPATTSSP 131

[Close]

BLAST of Lag0019754 vs. NCBI nr
Match: KAG7027509.1 (hypothetical protein SDJN02_11523, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 171.8 bits (434), Expect = 4.0e-39
Identity = 105/133 (78.95%), Postives = 111/133 (83.46%), Query Frame = 0


Query: 1   MASSKYLIIAFFMATLVFSTIDTGRAARHLLQTAPPNLPGLPSLPKPTALPPLPPLPTMP 60
           MASS YLIIA FMATLVFS IDTGRAARHLLQ A P++PGLPS      LPPLPPLP   
Sbjct: 1   MASSNYLIIA-FMATLVFSAIDTGRAARHLLQIASPSVPGLPS------LPPLPPLP--- 60

Query: 61  TTPTSGPGIPSIPNLPQPTLPTTQPSLPKLPPFPSFP-GIPTGMALPPLPTNLLPNLPSI 120
             PT+G GIPSIP+LPQPTLPTTQPSLPKLPPFP+FP  IPTGMALPPLPT LLPNLPSI
Sbjct: 61  -NPTTGSGIPSIPSLPQPTLPTTQPSLPKLPPFPTFPSNIPTGMALPPLPTTLLPNLPSI 120

Query: 121 PFLTPPPATTSSP 133
           P LTPPP T+SSP
Sbjct: 121 PSLTPPPTTSSSP 122

[Close]

BLAST of Lag0019754 vs. ExPASy Swiss-Prot
Match: P9WJD2 (ESX-1 secretion-associated protein EspE OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=espE PE=3 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 1.9e-04
Identity = 47/99 (47.47%), Postives = 51/99 (51.52%), Query Frame = 0


Query: 30  LLQTAP--PNLPGLPSLPKPTALPPLPPLPTMPTTPTSGPGIPSIPNLPQPTLPTT--QP 89
           +L T P  P LPGLPSLP        P LP +P      PG+P IP LP    P T   P
Sbjct: 174 MLTTLPKFPGLPGLPSLPDIIDGLWPPKLPDIPI-----PGLPDIPGLPDFKWPPTPGSP 233

Query: 90  SLPKLPPFPSFPGIPTGMALPPLPTNLLPNLPSIPFLTP 125
             P LP FP FPG P   A+P  P   LP LPSIP L P
Sbjct: 234 LFPDLPSFPGFPGFPEFPAIPGFPA--LPGLPSIPNLFP 265

[Close]

BLAST of Lag0019754 vs. ExPASy Swiss-Prot
Match: P9WJD3 (ESX-1 secretion-associated protein EspE OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=espE PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 1.9e-04
Identity = 47/99 (47.47%), Postives = 51/99 (51.52%), Query Frame = 0


Query: 30  LLQTAP--PNLPGLPSLPKPTALPPLPPLPTMPTTPTSGPGIPSIPNLPQPTLPTT--QP 89
           +L T P  P LPGLPSLP        P LP +P      PG+P IP LP    P T   P
Sbjct: 174 MLTTLPKFPGLPGLPSLPDIIDGLWPPKLPDIPI-----PGLPDIPGLPDFKWPPTPGSP 233

Query: 90  SLPKLPPFPSFPGIPTGMALPPLPTNLLPNLPSIPFLTP 125
             P LP FP FPG P   A+P  P   LP LPSIP L P
Sbjct: 234 LFPDLPSFPGFPGFPEFPAIPGFPA--LPGLPSIPNLFP 265

The following BLAST results are available for this feature:

BLAST of Lag0019754 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023518060.1	1.0e-42	82.71	proline-rich receptor-like protein kinase PERK2 [Cucurbita pepo subsp. pepo]	[more]
XP_023518061.1	1.5e-41	81.62	protein PELPK1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6595524.1	6.1e-40	80.30	hypothetical protein SDJN03_12077, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038904304.1	1.4e-39	76.60	RNA-binding protein 12-like [Benincasa hispida]	[more]
KAG7027509.1	4.0e-39	78.95	hypothetical protein SDJN02_11523, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Lag0019754 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P9WJD2	1.9e-04	47.47	ESX-1 secretion-associated protein EspE OS=Mycobacterium tuberculosis (strain CD...	[more]
P9WJD3	1.9e-04	47.47	ESX-1 secretion-associated protein EspE OS=Mycobacterium tuberculosis (strain AT...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 110..132
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 38..104
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 33..132
None	No IPR available	PANTHER	PTHR33088:SF33	PROTEIN APP1-LIKE	coord: 1..130
IPR044659	Protein PELPK-like	PANTHER	PTHR33088	MUCIN-2	coord: 1..130

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0019754.1	Lag0019754.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0016301	kinase activity