Lag0019687 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0019687
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr5: 44459359 .. 44460305 (-)
RNA-Seq ExpressionLag0019687
SyntenyLag0019687
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGGAGGACACAATGAAAACTTGCCTGGGTATTCTGAACAATAGGGAAGAGATAGAGCCTATAAACAAGACTCTGATTGCTCTTATTCCTAAATCCAAGGATCCAAAGACAATGAGTGAGTTTAGGTCAATCAGCCTGTGCAATGTGATTTACAAAGTGATAACAAAAGCCCTAGCGAATCGCATGAAACAACTGCTTGAATCTATCATATCCCCATCCCAATTAGCTTCGTCCCCGGAAGACAGATCACAGATAATGTGTTGGTGGGTTCGAGTGTATCCACGCTCTAAACAACAAAGAATAGGGAAAACAGGATATGTGGCTATAAAACTCGACATGAGTAAAGCCTACGATCGCGTGGAATGGGTGTATATAGAAGAAAGCATGAAACAAATGGGCTTCAGCCCCTGTTGGATCCAGAAAGTTATGAGTTGTATCTCCTCTGTGAGCTACTCTGTTCTAGTCAATGGCGAGCAACAAGAAGATTTTAAGCCGAGTAGGGGCCTTCGTCAGAGAGACCCCCTATCGCCCTACCTCTTCTTGCTGTGTGCGGAAGGTTTCTCGGCCCTCCTCGATAGGGAAGAATCTTTAGCTAAAATCTCTGGTTTTAAAATAAACAAATATTGTCCCACGCTAACCCATCTCTTTTTTGCAGATGACAGTCTTGTTTTCCTTAAAGCTAGGAGTCGGTCCTATGTACGTTCAAGCAAATCCTGAAACAATACGAGGGAGCCTCAGGCCAAACAATCAACCTTGAAAAGTCCATCTTCATGGCGAGCAAAAATGTTAAAGAGGAGACTCTGGCTAAGTGTGAGGTAGCTCTCGGGATCAAGAGATCGAATTCGTTAGGCCAATATCTGGGGATGCCATCTCAAACAGGAAGAAACAAAGGAGCAGTTTTCAAAAGAATCAAAGACAGGGTTGAGAAAATTCTTCAAGGATGA

mRNA sequence

ATGAGGGAGGACACAATGAAAACTTGCCTGGGTATTCTGAACAATAGGGAAGAGATAGAGCCTATAAACAAGACTCTGATTGCTCTTATTCCTAAATCCAAGGATCCAAAGACAATGAGTGAGTTTAGGTCAATCAGCCTGTGCAATGTGATTTACAAAGTGATAACAAAAGCCCTAGCGAATCGCATGAAACAACTGCTTGAATCTATCATATCCCCATCCCAATTAGCTTCGTCCCCGGAAGACAGATCACAGATAATGTGTTGGTGGGTTCGAGTGTATCCACGCTCTAAACAACAAAGAATAGGGAAAACAGGATATGTGGCTATAAAACTCGACATGAGTAAAGCCTACGATCGCGTGGAATGGGTGTATATAGAAGAAAGCATGAAACAAATGGGCTTCAGCCCCTGTTGGATCCAGAAAGTTATGAGTTGTATCTCCTCTGTGAGCTACTCTGTTCTAGTCAATGGCGAGCAACAAGAAGATTTTAAGCCGAGTAGGGGCCTTCGTCAGAGAGACCCCCTATCGCCCTACCTCTTCTTGCTGTGTGCGGAAGGCCAAACAATCAACCTTGAAAAGTCCATCTTCATGGCGAGCAAAAATGTTAAAGAGGAGACTCTGGCTAAGTGTGAGGTAGCTCTCGGGATCAAGAGATCGAATTCGTTAGGCCAATATCTGGGGATGCCATCTCAAACAGGAAGAAACAAAGGAGCAGTTTTCAAAAGAATCAAAGACAGGGTTGAGAAAATTCTTCAAGGATGA

Coding sequence (CDS)

ATGAGGGAGGACACAATGAAAACTTGCCTGGGTATTCTGAACAATAGGGAAGAGATAGAGCCTATAAACAAGACTCTGATTGCTCTTATTCCTAAATCCAAGGATCCAAAGACAATGAGTGAGTTTAGGTCAATCAGCCTGTGCAATGTGATTTACAAAGTGATAACAAAAGCCCTAGCGAATCGCATGAAACAACTGCTTGAATCTATCATATCCCCATCCCAATTAGCTTCGTCCCCGGAAGACAGATCACAGATAATGTGTTGGTGGGTTCGAGTGTATCCACGCTCTAAACAACAAAGAATAGGGAAAACAGGATATGTGGCTATAAAACTCGACATGAGTAAAGCCTACGATCGCGTGGAATGGGTGTATATAGAAGAAAGCATGAAACAAATGGGCTTCAGCCCCTGTTGGATCCAGAAAGTTATGAGTTGTATCTCCTCTGTGAGCTACTCTGTTCTAGTCAATGGCGAGCAACAAGAAGATTTTAAGCCGAGTAGGGGCCTTCGTCAGAGAGACCCCCTATCGCCCTACCTCTTCTTGCTGTGTGCGGAAGGCCAAACAATCAACCTTGAAAAGTCCATCTTCATGGCGAGCAAAAATGTTAAAGAGGAGACTCTGGCTAAGTGTGAGGTAGCTCTCGGGATCAAGAGATCGAATTCGTTAGGCCAATATCTGGGGATGCCATCTCAAACAGGAAGAAACAAAGGAGCAGTTTTCAAAAGAATCAAAGACAGGGTTGAGAAAATTCTTCAAGGATGA

Protein sequence

MREDTMKTCLGILNNREEIEPINKTLIALIPKSKDPKTMSEFRSISLCNVIYKVITKALANRMKQLLESIISPSQLASSPEDRSQIMCWWVRVYPRSKQQRIGKTGYVAIKLDMSKAYDRVEWVYIEESMKQMGFSPCWIQKVMSCISSVSYSVLVNGEQQEDFKPSRGLRQRDPLSPYLFLLCAEGQTINLEKSIFMASKNVKEETLAKCEVALGIKRSNSLGQYLGMPSQTGRNKGAVFKRIKDRVEKILQG
Homology
BLAST of Lag0019687 vs. NCBI nr
Match: XP_012461392.1 (PREDICTED: uncharacterized protein LOC105781396 [Gossypium raimondii])

HSP 1 Score: 216.5 bits (550), Expect = 2.7e-52
Identity = 122/310 (39.35%), Postives = 165/310 (53.23%), Query Frame = 0

Query: 3   EDTMKTCLGILNNREEIEPINKTLIALIPKSKDPKTMSEFRSISLCNVIYKVITKALANR 62
           +D +  CL ILN   EI PIN T I LIPK  +P TM +FR ISLCNV+YK+I K LANR
Sbjct: 169 DDVLSFCLQILNGNMEISPINTTNIVLIPKVSNPSTMMQFRPISLCNVLYKIIAKVLANR 228

Query: 63  MKQLLESIISPSQLASSPEDR-SQIMCWWVRVYPRSKQQRIGKTGYVAIKLDMSKAYDRV 122
            + ++   I  +Q A  P    S  +     +  + KQ+R GK G++A+KLDMSKAYDRV
Sbjct: 229 FRSVIGKCIDEAQSAFVPGRLISDNVLLTYEILHKLKQKRTGKKGFMAVKLDMSKAYDRV 288

Query: 123 EWVYIEESMKQMGFSPCWIQKVMSCISSVSYSVLVNGEQQEDFKPSRGLRQRDPLSPYLF 182
           EW  IEE MK+MGF   W+  +M C+SSV+YSV++NG + + F P+RGLRQ DPLSP+LF
Sbjct: 289 EWKLIEEVMKKMGFDFSWVNAIMKCVSSVAYSVVINGYRGDKFVPTRGLRQGDPLSPFLF 348

Query: 183 LLCAE------------------------------------------------------- 242
           L+C E                                                       
Sbjct: 349 LICGEGLSSLLRLAMKRDRLKGVRVSRYGPQVSHLLFADDCILFGEATSRGANIIKDILC 408

Query: 243 ------GQTINLEKSIFMASKNVKEETLAKCEVALGIKRSNSLGQYLGMPSQTGRNKGAV 251
                 GQ +N +KS    SKN  EE   +    LG++R++   +YLG+P+  GR K   
Sbjct: 409 EFKVCSGQCVNFDKSTVFFSKNTLEEDRGQVVDVLGVRRTSEPERYLGLPNMVGRKKKES 468

BLAST of Lag0019687 vs. NCBI nr
Match: KAA3465168.1 (reverse transcriptase [Gossypium australe])

HSP 1 Score: 208.0 bits (528), Expect = 9.6e-50
Identity = 120/281 (42.70%), Postives = 154/281 (54.80%), Query Frame = 0

Query: 3   EDTMKTCLGILNNREEIEPINKTLIALIPKSKDPKTMSEFRSISLCNVIYKVITKALANR 62
           +D  + CL ILN ++EI  INKT I LIPK  +PK MS FR ISLCNVIYK+I K L N+
Sbjct: 108 QDISRYCLDILNGKKEIGDINKTRIVLIPKIDNPKNMSHFRPISLCNVIYKIIAKVLVNQ 167

Query: 63  MKQLLESIISPSQLASSPEDR-SQIMCWWVRVYPRSKQQRIGKTGYVAIKLDMSKAYDRV 122
           M Q+L   I+ +Q A  P    S  +     V    K +R G+ G   +KLDMSKAYD V
Sbjct: 168 MSQILGDCINEAQGAFIPGRLISDSVLIAYEVLHSLKMKRSGRRGNFTLKLDMSKAYDHV 227

Query: 123 EWVYIEESMKQMGFSPCWIQKVMSCISSVSYSVLVNGEQQEDFKPSRGLRQRDPLSPYLF 182
           EW Y+   MK +GF   W+  +M C++SVSYSV +NG +   F P RGLRQ DPL+PYLF
Sbjct: 228 EWDYLAGMMKSLGFHDDWMVLIMRCVTSVSYSVSLNGMRSNWFSPLRGLRQGDPLNPYLF 287

Query: 183 LLCAE----------------------------GQTINLEKSIFMASKNVKEETLAKCEV 242
           L+CAE                            GQ +N  KS+     NVKEE       
Sbjct: 288 LICAEDDCIIFGDATHVGVSTVRDIIREYEMSAGQKVNYNKSLIYFGANVKEEIKGDIIR 347

Query: 243 ALGIKRSNSLGQYLGMPSQTGRNKGAVFKRIKDRVEKILQG 255
            LG++ +++  +YLG+P   GR K   F   KDR  K + G
Sbjct: 348 TLGVRVASNRKKYLGLPMMVGRRKAWAFASFKDRFRKRVDG 388

BLAST of Lag0019687 vs. NCBI nr
Match: KAA3468713.1 (reverse transcriptase [Gossypium australe])

HSP 1 Score: 206.8 bits (525), Expect = 2.1e-49
Identity = 124/285 (43.51%), Postives = 155/285 (54.39%), Query Frame = 0

Query: 9   CLGILNNREEIEPINKTLIALIPKSKDPKTMSEFRSISLCNVIYKVITKALANRMKQLLE 68
           CL ILN ++EI  INKT I LIPK  +PK MS F+ ISLCNVIYK+I K L NRM  +L 
Sbjct: 196 CLDILNEQKEIGDINKTRIVLIPKIDNPKNMSYFQPISLCNVIYKIIPKVLVNRMSDILG 255

Query: 69  SIISPSQLASSP----EDRSQIMCWWVRVYPRSKQQRIGKTGYVAIKLDMSKAYDRVEWV 128
             I+ +Q A  P     D   I C    V    K ++ G+ G  A+KLDMSKAYDRVEW 
Sbjct: 256 DCINEAQGAFIPGKLISDNVLIAC---EVLHSLKMKKNGRRGNFALKLDMSKAYDRVEWD 315

Query: 129 YIEESMKQMGFSPCWIQKVMSCISSVSYSVLVNGEQQEDFKPSRGLRQRDPLSPYLFLLC 188
           ++   MK +GF   W+  +M CI+SVSYSV +NG   + F PSRGLRQ DPLSPYLFLLC
Sbjct: 316 FLAGMMKSLGFHNDWMVLIMRCITSVSYSVSLNGMCSDWFSPSRGLRQGDPLSPYLFLLC 375

Query: 189 AE-----------------------------------GQTINLEKSIFMASKNVKEETLA 248
           AE                                   GQ +N +KS+     NVKEE   
Sbjct: 376 AEGFSTLLEDAKQKGRMRGASGVCTVRYIIREYEMSAGQKVNYDKSLIYFGANVKEEVNE 435

Query: 249 KCEVALGIKRSNSLGQYLGMPSQTGRNKGAVFKRIKDRVEKILQG 255
                LG++ ++S  +YLG+P   GR K   F   KD+  K + G
Sbjct: 436 DIIRVLGVRVASSPEKYLGLPMMVGRRKAWAFANFKDKFRKRVDG 477

BLAST of Lag0019687 vs. NCBI nr
Match: XP_042952177.1 (uncharacterized protein LOC122289261 [Carya illinoinensis])

HSP 1 Score: 205.7 bits (522), Expect = 4.8e-49
Identity = 115/258 (44.57%), Postives = 163/258 (63.18%), Query Frame = 0

Query: 3   EDTMKTCLGILNNREEIEPINKTLIALIPKSKDPKTMSEFRSISLCNVIYKVITKALANR 62
           E+  K  L ILN   EI  IN+T I LIPK K+P+T+ +F+ ISLCNVIYKV++K L+NR
Sbjct: 389 EEVTKAVLDILNGNGEISHINETYIVLIPKVKNPQTVMDFKPISLCNVIYKVVSKTLSNR 448

Query: 63  MKQLLESIISPSQLA--SSPEDRSQIMCWWVRVYPRSKQQRIGKTGYVAIKLDMSKAYDR 122
           +K +L SII+P+Q A       +  ++  +  ++  S + + G+ GY+AIKLD+SKA+DR
Sbjct: 449 LKLILPSIIAPTQSAFIQGRMIQDNVIVAFETLHSMSIKGK-GQQGYMAIKLDLSKAFDR 508

Query: 123 VEWVYIEESMKQMGFSPCWIQKVMSCISSVSYSVLVNGEQQEDFKPSRGLRQRDPLSPYL 182
           VEW ++ + M +MGF+  WI   M C+++VSYS L+NG+ Q +F PSRG+RQ DPLSPYL
Sbjct: 509 VEWEFLAKVMAKMGFNRRWIFLTMQCVTTVSYSTLINGKPQGNFSPSRGIRQGDPLSPYL 568

Query: 183 FLLCAE----------GQTINLEKSIFMASKNVKEETLAKCEVALGIKRSNSLGQYLGMP 242
           F+L AE          GQ +N  K+    S N +  T          + S+   +YLG+P
Sbjct: 569 FILVAEVILNLYEEASGQKLNKSKTSIYFSPNTRPATREYILSLAQTRASSCYERYLGLP 628

Query: 243 SQTGRNKGAVFKRIKDRV 249
           +  GR+K A FK I DRV
Sbjct: 629 NLIGRSKYAAFKGILDRV 645

BLAST of Lag0019687 vs. NCBI nr
Match: XP_028068804.1 (uncharacterized protein LOC114271378 [Camellia sinensis])

HSP 1 Score: 205.3 bits (521), Expect = 6.2e-49
Identity = 125/310 (40.32%), Postives = 165/310 (53.23%), Query Frame = 0

Query: 7   KTCLGILNNREEIEPINKTLIALIPKSKDPKTMSEFRSISLCNVIYKVITKALANRMKQL 66
           +  LG+LN  + +  +N T I LIPK K PK MS+FR ISLCNV+YK+++K LANRM+++
Sbjct: 412 RAVLGVLNEGKAVTAMNDTFIVLIPKVKSPKRMSQFRPISLCNVVYKLVSKVLANRMRKI 471

Query: 67  LESIISPSQLA-SSPEDRSQIMCWWVRVYPRSKQQRIGKTGYVAIKLDMSKAYDRVEWVY 126
           L  IIS +Q A  +    S  M     ++   K +R GK G+ A+KLDMSKAYDRVEW +
Sbjct: 472 LPDIISMNQSAFVAGRLISDNMLASFEIFHFLKNKRHGKEGHFALKLDMSKAYDRVEWSF 531

Query: 127 IEESMKQMGFSPCWIQKVMSCISSVSYSVLVNGEQQEDFKPSRGLRQRDPLSPYLFLLCA 186
           +   M++MGF+  ++  ++ CISSVSYSVLVNG   + F P+RGLRQ DPLSPYLF+LCA
Sbjct: 532 LRGVMERMGFNQLFVDTIVHCISSVSYSVLVNGSPIKKFVPTRGLRQGDPLSPYLFVLCA 591

Query: 187 E----------------------------------------------------------- 246
           E                                                           
Sbjct: 592 EGLSALIKRAEGEGKLTGVAVCRGSPRVSHLLFADDSLLFGAANMQELVVVQDILGKYEL 651

Query: 247 --GQTINLEKSIFMASKNVKEETLAKCEVALGIKRSNSLGQYLGMPSQTGRNKGAVFKRI 255
             GQ INLEKS    SKNV  +  A    A+G+      G+YLG+P   GR+K  +F  +
Sbjct: 652 VSGQKINLEKSAICFSKNVGIDIQAGLRNAMGVGNLVDSGKYLGLPYIVGRSKRGIFNSV 711

BLAST of Lag0019687 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 1.0e-14
Identity = 63/222 (28.38%), Postives = 105/222 (47.30%), Query Frame = 0

Query: 27  IALIPK-SKDPKTMSEFRSISLCNVIYKVITKALANRMKQLLESIISPSQLASSPEDRSQ 86
           I LIPK  KDP  +  FR ISL N+  K++ K LANR+++ +++II P Q+   P     
Sbjct: 521 ITLIPKPQKDPTKIENFRPISLMNIDAKILNKILANRIQEHIKAIIHPDQVGFIPG---- 580

Query: 87  IMCWWVRVYPRSKQ----QRIGKTGYVAIKLDMSKAYDRVEWVYIEESMKQMGFSPCWIQ 146
            M  W  +           ++    ++ I LD  KA+D+++  ++ + +++ G    ++ 
Sbjct: 581 -MQGWFNIRKSINVIHYINKLKDKNHMIISLDAEKAFDKIQHPFMIKVLERSGIQGPYLN 640

Query: 147 KVMSCISSVSYSVLVNGEQQEDFKPSRGLRQRDPLSPYLFLLCAE--GQTINLE---KSI 206
            + +  S    ++ VNGE+ E      G RQ  PLSPYLF +  E   + I  +   K I
Sbjct: 641 MIKAIYSKPVANIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVLARAIRQQKEIKGI 700

Query: 207 FMASKNVKEETLAKCEVALGIKRSNSLGQYLGMPSQTGRNKG 239
            +  + VK   LA   +       NS  + L + +  G   G
Sbjct: 701 QIGKEEVKISLLADDMIVYISDPKNSTRELLNLINSFGEVVG 737

BLAST of Lag0019687 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 4.4e-13
Identity = 57/216 (26.39%), Postives = 105/216 (48.61%), Query Frame = 0

Query: 2   REDTMKTCLGILNNREEIEPINKTL----IALIPK-SKDPKTMSEFRSISLCNVIYKVIT 61
           +E+ +   L +  N E+   +  T     I LIPK  KDP     +R ISL N+  K++ 
Sbjct: 484 KEELVPILLNLFQNIEKEGILPNTFYEANITLIPKPGKDPTRKENYRPISLMNIDAKILN 543

Query: 62  KALANRMKQLLESIISPSQLASSPEDRSQIMCWWVRVYPRSKQQRIGK---TGYVAIKLD 121
           K L NR++Q ++ II   Q+   P  +     W+      +  Q I K     ++ + +D
Sbjct: 544 KILTNRIQQHIKKIIHHDQVGFIPGSQG----WFNIRKSINVIQHINKLKNKDHMILSID 603

Query: 122 MSKAYDRVEWVYIEESMKQMGFSPCWIQKVMSCISSVSYSVLVNGEQQEDFKPSRGLRQR 181
             KA+D ++  ++  ++K++G    +++ + +  S  + ++++NG + + F    G RQ 
Sbjct: 604 AEKAFDNIQHPFMIRTLKKIGIEGTFLKLIEAIYSKPTANIILNGVKLKSFPLRSGTRQG 663

Query: 182 DPLSPYLFLLCAEGQTINLE-----KSIFMASKNVK 205
            PLSP LF +  E   I +      K I + S+ +K
Sbjct: 664 CPLSPLLFNIVMEVLAIAIREEKAIKGIHIGSEEIK 695

BLAST of Lag0019687 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.3e-12
Identity = 59/215 (27.44%), Postives = 104/215 (48.37%), Query Frame = 0

Query: 27  IALIPK-SKDPKTMSEFRSISLCNVIYKVITKALANRMKQLLESIISPSQLASSPEDRSQ 86
           I LIPK  +D      FR ISL N+  K++ K LANR++Q ++ +I   Q+   P     
Sbjct: 514 IILIPKPGRDTTKKENFRPISLMNIDAKILNKILANRIQQHIKKLIHHDQVGFIPG---- 573

Query: 87  IMCWWVRVYPR----SKQQRIGKTGYVAIKLDMSKAYDRVEWVYIEESMKQMGFSPCWIQ 146
            M  W  +           R     +V I +D  KA+D+++  ++ +++ ++G    +++
Sbjct: 574 -MQGWFNIRKSINVIQHINRAKDKNHVIISIDAEKAFDKIQQPFMLKTLNKLGIDGMYLK 633

Query: 147 KVMSCISSVSYSVLVNGEQQEDFKPSRGLRQRDPLSPYLFLLCAEGQTINLEKSIFMASK 206
            + +     + ++++NG++ E F    G RQ  PLSP LF +  E     L ++I    K
Sbjct: 634 IIRAIYDKPTANIILNGQKLEAFPLKTGTRQGCPLSPLLFNIVLE----VLARAI-RQEK 693

Query: 207 NVKEETLAKCEVALGIKRSNSLGQYLGMPSQTGRN 237
            +K   L K EV L +   + +  YL  P  + +N
Sbjct: 694 EIKGIQLGKEEVKLSLFADDMI-VYLENPIVSAQN 717

BLAST of Lag0019687 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 5.9e-10
Identity = 44/163 (26.99%), Postives = 80/163 (49.08%), Query Frame = 0

Query: 24  KTLIALIPKSKDPKTMSEFRSISLCNVIYKVITKALANRMKQLLESIISPSQLASSPEDR 83
           + +++L+PK  D + +  +R +SL +  YK++ KA++ R+K +L  +I P Q  + P   
Sbjct: 507 RAVLSLLPKKGDLRLIKNWRPVSLLSTDYKIVAKAISLRLKSVLAEVIHPDQSYTVPGRT 566

Query: 84  SQIMCWWVRVYPRSKQQRIGKTGYVAIKLDMSKAYDRVEWVYIEESMKQMGFSPCWIQKV 143
                + +R       +R G      + LD  KA+DRV+  Y+  +++   F P ++  +
Sbjct: 567 IFDNVFLIRDLLHF-ARRTG-LSLAFLSLDQEKAFDRVDHQYLIGTLQAYSFGPQFVGYL 626

Query: 144 MSCISSVSYSVLVNGEQQEDFKPSRGLRQRDPLSPYLFLLCAE 187
            +  +S    V +N          RG+RQ  PLS  L+ L  E
Sbjct: 627 KTMYASAECLVKINWSLTAPLAFGRGVRQGCPLSGQLYSLAIE 667

BLAST of Lag0019687 vs. ExPASy Swiss-Prot
Match: P16423 (Retrovirus-related Pol polyprotein from type-2 retrotransposable element R2DM OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 2.5e-08
Identity = 45/154 (29.22%), Postives = 75/154 (48.70%), Query Frame = 0

Query: 30  IPKSKDPKTMSEFRSISLCNVIYKVITKALANRMKQLLESIISPSQLASSPEDRSQIMCW 89
           IPK+   K   +FR IS+ +V+ + +   LA R+   +     P Q    P D       
Sbjct: 403 IPKTVTAKRPQDFRPISVPSVLVRQLNAILATRLNSSIN--WDPRQRGFLPTDGCADNAT 462

Query: 90  WVRVYPRSKQQRIGKTGYVAIKLDMSKAYDRVEWVYIEESMKQMGFSPCWIQKVMSCISS 149
            V +  R   +   ++ Y+A  LD+SKA+D +    I ++++  G    ++  V +    
Sbjct: 463 IVDLVLRHSHKHF-RSCYIA-NLDVSKAFDSLSHASIYDTLRAYGAPKGFVDYVQNTYEG 522

Query: 150 VSYSVLVNGEQQEDFKPSRGLRQRDPLSPYLFLL 184
              S+  +G   E+F P+RG++Q DPLSP LF L
Sbjct: 523 GGTSLNGDGWSSEEFVPARGVKQGDPLSPILFNL 552

BLAST of Lag0019687 vs. ExPASy TrEMBL
Match: M5XK32 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa016563mg PE=4 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 2.5e-51
Identity = 120/287 (41.81%), Postives = 165/287 (57.49%), Query Frame = 0

Query: 3   EDTMKTCLGILNNREEIEPINKTLIALIPKSKDPKTMSEFRSISLCNVIYKVITKALANR 62
           +D +      L + + +  +N T + LIPK K+P+TM++ R ISLCNV+Y++  K LANR
Sbjct: 64  DDVVAAIRAFLQSNDMLRQLNHTFVTLIPKVKEPRTMAQLRPISLCNVLYRIGAKTLANR 123

Query: 63  MKQLLESIISPSQLASSP----EDRSQIMCWWVRVYPRSKQQRIGKTGYVAIKLDMSKAY 122
           MK +++S+IS SQ A  P     D S +      +    KQ+R G+ G +A+KLDMSKAY
Sbjct: 124 MKFVMQSVISESQSAFVPGRLITDNSIVA---FEIAHFLKQRRRGRKGSLALKLDMSKAY 183

Query: 123 DRVEWVYIEESMKQMGFSPCWIQKVMSCISSVSYSVLVNGEQQEDFKPSRGLRQRDPLSP 182
           DRVEW ++E+ M  MGF   W++ VM C+++VSYS LVNGE      P+RGLRQ DPLSP
Sbjct: 184 DRVEWEFLEKMMLAMGFPILWVRMVMDCVTTVSYSFLVNGEPTRILYPTRGLRQGDPLSP 243

Query: 183 YLFLLCAE-------------------------------GQTINLEKSIFMASKNVKEET 242
           YLFLLCAE                               G+ IN +KS    S N+  +T
Sbjct: 244 YLFLLCAEVLPHDSFVFAKATDNNCGVLKHIFEVYERASGEQINCQKSCVAFSANIHMDT 303

Query: 243 LAKCEVALGIKRSNSLGQYLGMPSQTGRNKGAVFKRIKDRVEKILQG 255
            ++    LG+ R +S   YLG+P   GRNK   F+ +K+RV K LQG
Sbjct: 304 QSRLASVLGVPRVDSHATYLGLPMMLGRNKTFCFRYLKERVWKKLQG 347

BLAST of Lag0019687 vs. ExPASy TrEMBL
Match: A0A2N9J6I3 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS60908 PE=4 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 4.2e-51
Identity = 119/278 (42.81%), Postives = 159/278 (57.19%), Query Frame = 0

Query: 4    DTMKTCLGILNNREEIEPINKTLIALIPKSKDPKTMSEFRSISLCNVIYKVITKALANRM 63
            D   + L  LNN   ++ +N T IALIPK K P+ M++FR ISLCNV+YK+I+K L NRM
Sbjct: 963  DVTDSILDFLNNGHMLKSLNYTHIALIPKVKSPELMTQFRPISLCNVLYKIISKVLVNRM 1022

Query: 64   KQLLESIISPSQLASSPEDR-SQIMCWWVRVYPRSKQQRIGKTGYVAIKLDMSKAYDRVE 123
            K +L  ++S SQ A  P    S  +     +    K +R+GK   +A+KLDMSKAYDRVE
Sbjct: 1023 KTILPFVVSDSQSAFVPGRMISDNIMIAFEILHHLKNKRVGKVPQMAVKLDMSKAYDRVE 1082

Query: 124  WVYIEESMKQMGFSPCWIQKVMSCISSVSYSVLVNGEQQEDFKPSRGLRQRDPLSPYLFL 183
            W Y+++ M ++GF+  W+  +M C++SVSYS+LVNGE +   KPSRGLRQ DPLSPYLFL
Sbjct: 1083 WDYLKKMMLKLGFTARWVALIMECVTSVSYSILVNGEPKGYVKPSRGLRQGDPLSPYLFL 1142

Query: 184  LCAE--------------------------GQTINLEKSIFMASKNVKEETLAKCEVALG 243
            +CAE                          GQ IN  K+    S+N      A      G
Sbjct: 1143 ICAEGLTALLRKAERESVMQGISICRGGPQGQKINTAKTALFFSQNASPIVKASILNFFG 1202

Query: 244  IKRSNSLGQYLGMPSQTGRNKGAVFKRIKDRVEKILQG 255
               +    +YLG+P   GR+K   F  IKDR+ + LQG
Sbjct: 1203 TSPTTQFEKYLGLPPVIGRSKKQAFSEIKDRIWRRLQG 1240

BLAST of Lag0019687 vs. ExPASy TrEMBL
Match: A0A2N9J6K4 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS59981 PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 5.5e-51
Identity = 119/279 (42.65%), Postives = 159/279 (56.99%), Query Frame = 0

Query: 4   DTMKTCLGILNNREEIEPINKTLIALIPKSKDPKTMSEFRSISLCNVIYKVITKALANRM 63
           D   + L  LNN   ++ +N T IALIPK K P+ M++FR ISLCNV+YK+I+K L NRM
Sbjct: 349 DVTDSILDFLNNGHMLKSLNYTHIALIPKVKSPELMTQFRPISLCNVLYKIISKVLVNRM 408

Query: 64  KQLLESIISPSQLASSPEDR-SQIMCWWVRVYPRSKQQRIGKTGYVAIKLDMSKAYDRVE 123
           K +L  ++S SQ A  P    S  +     +    K +R+GK   +A+KLDMSKAYDRVE
Sbjct: 409 KTILPFVVSDSQSAFVPGRMISDNIMIAFEILHHLKNKRVGKVPQMAVKLDMSKAYDRVE 468

Query: 124 WVYIEESMKQMGFSPCWIQKVMSCISSVSYSVLVNGEQQEDFKPSRGLRQRDPLSPYLFL 183
           W Y+++ M ++GF+  W+  +M C++SVSYS+LVNGE +   KPSRGLRQ DPLSPYLFL
Sbjct: 469 WDYLKKMMLKLGFTARWVALIMECVTSVSYSILVNGEPKGYVKPSRGLRQGDPLSPYLFL 528

Query: 184 LCAE---------------------------GQTINLEKSIFMASKNVKEETLAKCEVAL 243
           +CAE                           GQ IN  K+    S+N      A      
Sbjct: 529 ICAEGLTAFIAATDTECQALQDILTLYESASGQKINTAKTALFFSQNASPIVKASILNFF 588

Query: 244 GIKRSNSLGQYLGMPSQTGRNKGAVFKRIKDRVEKILQG 255
           G   +    +YLG+P   GR+K   F  IKDR+ + LQG
Sbjct: 589 GTSPTTQFEKYLGLPPVIGRSKKQAFSEIKDRIWRRLQG 627

BLAST of Lag0019687 vs. ExPASy TrEMBL
Match: A0A2N9EK17 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS7109 PE=4 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 7.2e-51
Identity = 121/290 (41.72%), Postives = 164/290 (56.55%), Query Frame = 0

Query: 4   DTMKTCLGILNNREEIEPINKTLIALIPKSKDPKTMSEFRSISLCNVIYKVITKALANRM 63
           D +   L  L +   ++ +N T IALIPK    +++S+FR ISLCNV+YK+I+K LANRM
Sbjct: 27  DVLTAVLDCLRSGHILKSLNSTNIALIPKVGKAESLSQFRPISLCNVVYKIISKVLANRM 86

Query: 64  KQLLESIISPSQLASSPE---DRSQIMCWWVRVYPRSKQQRIGKTGYVAIKLDMSKAYDR 123
           K +L  +IS  Q A  PE     + ++ + V  Y  +K+Q  GKT ++A KLDMSKAYDR
Sbjct: 87  KSILSVVISDYQSAFVPERLITDNILIAFEVMHYLNTKRQ--GKTAHMAAKLDMSKAYDR 146

Query: 124 VEWVYIEESMKQMGFSPCWIQKVMSCISSVSYSVLVNGEQQEDFKPSRGLRQRDPLSPYL 183
           VEW Y++  M +MGF   W+  +M C+S+V+YSVL+NGE     +PSRGLRQ DPLSPYL
Sbjct: 147 VEWNYLQAVMLKMGFESRWVNLIMECLSTVNYSVLINGEPHGYIRPSRGLRQGDPLSPYL 206

Query: 184 FLLCAE------------------------------------GQTINLEKSIFMASKNVK 243
           FL+CAE                                    GQ +N EKS F  SKN  
Sbjct: 207 FLICAEGLSALFRAAERERRLCALQSDSMALKEILHNYEMASGQQLNFEKSAFFFSKNTP 266

Query: 244 EETLAKCEVALGIKRSNSLGQYLGMPSQTGRNKGAVFKRIKDRVEKILQG 255
           ++        LG   +++ G+YLG+P   GR K   F+  K R+ K LQG
Sbjct: 267 QQIKEDISTILGTSLTSNFGKYLGLPPLIGRKKRQAFEENKTRIWKRLQG 314

BLAST of Lag0019687 vs. ExPASy TrEMBL
Match: A0A5B6V7P4 (Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_000369 PE=4 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 4.7e-50
Identity = 120/281 (42.70%), Postives = 154/281 (54.80%), Query Frame = 0

Query: 3   EDTMKTCLGILNNREEIEPINKTLIALIPKSKDPKTMSEFRSISLCNVIYKVITKALANR 62
           +D  + CL ILN ++EI  INKT I LIPK  +PK MS FR ISLCNVIYK+I K L N+
Sbjct: 108 QDISRYCLDILNGKKEIGDINKTRIVLIPKIDNPKNMSHFRPISLCNVIYKIIAKVLVNQ 167

Query: 63  MKQLLESIISPSQLASSPEDR-SQIMCWWVRVYPRSKQQRIGKTGYVAIKLDMSKAYDRV 122
           M Q+L   I+ +Q A  P    S  +     V    K +R G+ G   +KLDMSKAYD V
Sbjct: 168 MSQILGDCINEAQGAFIPGRLISDSVLIAYEVLHSLKMKRSGRRGNFTLKLDMSKAYDHV 227

Query: 123 EWVYIEESMKQMGFSPCWIQKVMSCISSVSYSVLVNGEQQEDFKPSRGLRQRDPLSPYLF 182
           EW Y+   MK +GF   W+  +M C++SVSYSV +NG +   F P RGLRQ DPL+PYLF
Sbjct: 228 EWDYLAGMMKSLGFHDDWMVLIMRCVTSVSYSVSLNGMRSNWFSPLRGLRQGDPLNPYLF 287

Query: 183 LLCAE----------------------------GQTINLEKSIFMASKNVKEETLAKCEV 242
           L+CAE                            GQ +N  KS+     NVKEE       
Sbjct: 288 LICAEDDCIIFGDATHVGVSTVRDIIREYEMSAGQKVNYNKSLIYFGANVKEEIKGDIIR 347

Query: 243 ALGIKRSNSLGQYLGMPSQTGRNKGAVFKRIKDRVEKILQG 255
            LG++ +++  +YLG+P   GR K   F   KDR  K + G
Sbjct: 348 TLGVRVASNRKKYLGLPMMVGRRKAWAFASFKDRFRKRVDG 388

BLAST of Lag0019687 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 58.2 bits (139), Expect = 1.1e-08
Identity = 26/85 (30.59%), Postives = 50/85 (58.82%), Query Frame = 0

Query: 59  LANRMKQLLESIISPSQLASSPEDRSQIMCWWVRVYPRSKQQRIGKTGYVAIKLDMSKAY 118
           +  R+K L+ ++I P+Q +  P   S     +V+    S +++ G  G++ +KLD+ KAY
Sbjct: 1   MVERLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSMRRKKGVKGWMLLKLDLEKAY 60

Query: 119 DRVEWVYIEESMKQMGFSPCWIQKV 144
           DR+ W Y+E+++   GF   W+ ++
Sbjct: 61  DRIRWDYLEDTLISAGFPEVWLPEI 85

BLAST of Lag0019687 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 48.1 bits (113), Expect = 1.2e-05
Identity = 21/32 (65.62%), Postives = 24/32 (75.00%), Query Frame = 0

Query: 155 LVNGEQQEDFKPSRGLRQRDPLSPYLFLLCAE 187
           ++NG  Q    PSRGLRQ DPLSPYLF+LC E
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTE 44

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_012461392.12.7e-5239.35PREDICTED: uncharacterized protein LOC105781396 [Gossypium raimondii][more]
KAA3465168.19.6e-5042.70reverse transcriptase [Gossypium australe][more]
KAA3468713.12.1e-4943.51reverse transcriptase [Gossypium australe][more]
XP_042952177.14.8e-4944.57uncharacterized protein LOC122289261 [Carya illinoinensis][more]
XP_028068804.16.2e-4940.32uncharacterized protein LOC114271378 [Camellia sinensis][more]
Match NameE-valueIdentityDescription
P113691.0e-1428.38LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE... [more]
P085484.4e-1326.39LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1[more]
O003701.3e-1227.44LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1[more]
P143815.9e-1026.99Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV... [more]
P164232.5e-0829.22Retrovirus-related Pol polyprotein from type-2 retrotransposable element R2DM OS... [more]
Match NameE-valueIdentityDescription
M5XK322.5e-5141.81Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=... [more]
A0A2N9J6I34.2e-5142.81Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS60908 PE=4 SV=1[more]
A0A2N9J6K45.5e-5142.65Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A2N9EK177.2e-5141.72Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A5B6V7P44.7e-5042.70Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_000369 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G20520.11.1e-0830.59RNA binding;RNA-directed DNA polymerases [more]
ATMG01250.11.2e-0565.63RNA-directed DNA polymerase (reverse transcriptase) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 31..188
e-value: 2.5E-23
score: 82.7
NoneNo IPR availablePANTHERPTHR19446REVERSE TRANSCRIPTASEScoord: 3..238
NoneNo IPR availablePANTHERPTHR19446:SF440SUBFAMILY NOT NAMEDcoord: 3..238
NoneNo IPR availableCDDcd01650RT_nLTR_likecoord: 24..209
e-value: 1.02977E-29
score: 109.303
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 27..185

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0019687.1Lag0019687.1mRNA