Lag0019674 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0019674
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Mediator of RNA polymerase II transcription subunit 28
Location	chr5: 44381544 .. 44384026 (-)
RNA-Seq Expression	Lag0019674
Synteny	Lag0019674

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGAACGACAACCACTCGACCAACAGCATCAACTTGATCCACAGTTGCAATCCTCGCAGTCTCCAAGAGAAGATATGATTGCATATGTGATGGCCTTGGAAGCTGCTCTGCTTCCGTGCTTGCCTGCTAGAGAACTACAAGCAATAGACCGTTCTCCTCACCCTTCTCATCAAAGTCAGTAATTGATCCTCTCCTTTGATGCCTGAGTTTCACTAGATTCTGAAAACACATTGCAAAACCAGTGATTGGGCTGGTTCCCTTTATACATTATCATATCTTCTTGCCTACCATGATATGATCTCTTGAATTGAATAAGTTATGTAAAAGAGTTTTTATGTGTATAACTGTGCTTACTAACAATTTTGCATGAAAACAGATCTAAAGTTTTGGTCGTTGTGGGTCTTCATAATGCAAACTCTTTGTTTTTGTTGGTGATTTCTTTACTAATCACGATGGTTTCACTAATCCTTGTTATTGACAAGAAAAAAAACAAGTGTTGCATTGATTTTCAAAATAATGGTTACTGGACCAAGTTTTGAAATTACTTAGGATTAAGAGAAACTTATTGATTTGATTTGATCTTGTTTTATTTTTTAGAAGAGAAACTTGTTGATGCAACAAACGATAAATTGATCCACTTGCGAAGAGTGATAGTTCAATGGCCATTGTTATCCCTAGAGATTTAGGAGGGTGTTTGATGGAGGGTTTGGCTTAAGATGCTGAATCTATGTTTGCATTGTAGATTTGTCAAGCTTGGGGTTTGTTGGACAAACCATGGGTTTCTAATTCCACTCCCTCCTCATACAATTTTCCCAAATTCAGGTTTTTAAATGTGTACCTCGAACATTGATTTCCTAAACCCAAGCTTCTCAAATCACTCACTTAAACTTTTGGCTTAATATCTCTAGGTTTAAGAAGCCGACCCCGTGCCTCAAATGGCTCCTGAATGTGTTAAACATGCAAACCATGAAAAGTCTGTTCTTGAGTTCCTTTCTTATTGAAGGGGCTAAAGGAGAACTATACTAGCATTTGTCCACAAAACTTTAACTTTGCCTTCAAACTACCAGCCTACAGAGCGATTATATAATAGTAAACTATCGTTTCCCCCGCTTTCTAAGTTCCCAGACCCAACAGAAAAAAAAAAAAAAAAAGCCCCGTGATTGGATGTTTTATGTTGTTCTAAGGAAATTAGGGTCTTTAAAGACATCCTATTCTGAAAAACTGATGTACAACAACAATCCTAAGCAGGCAGATGTATTCCCGATGCCTTCTTCAGCTTCTTGATAACTATAGTAGCATGGCATCATATGGATTGCTCCTGTTTATAATGGATACTTTAGCTAGTTCTCTATTTGTTTTGCAGACAGACAACTATGGGGGAACTGTCTCTTATATTGTTTCACTCAACTAGTTCATTGGCTCTTTTGACAATTTACTTCATTTTCTCTCAAGCACAACAATCTGTGGTGTAACTTGTCCGAGGGTAATTCTTCATATGAAAATCTGCGTTTGGCTTTTCTGAACTAGTTCATTTGTTCTTTAAATACTGCCCCTGGTCTAAAAAATTGATGTGCATTCCAGATTTTAGTGTTACACTAAGCTGACAGTTAGTGTGGTTTTCAGTTTCTTCATAGTTATTGTAACATCTTCTGGATCTGTTTATCAATGGATACCTTTGCAAGTTCACAAGTTATAAGGGAGCCGTCTCTTAGTATTTTTGCTCATTCAACTAGTTCATTTGCTCTTTTAATTTCCTTTCTTTGCTCTCCTCAAGCATTGCAATCTGTAAAGCAACTTACTTGACAGTAAATCTTCATGAAAATGGGTTCATCATACTCTATTTCATATTACTGGAGATCTGCAGCTGTATCTGGATCTAATGACTGATTGATCTTTAGTCATTAATTTTCTTGCATTAAAATTATGTGGATGTGTGATTTTCATTAATTTTTTGAATTGAAATATGTGGAGTGGAAGTGACATCTAGATCTAGAAGTAGCATAAATGTCTGCAATCTGCAACTCTAGTGGCTTTATGTACCAGGGGGAGCTGGGTGTTGAATTTCAATGTCTGTGGTTACATGTTTGTAAACAGTGAAAAAATGACAACCTGTTTTTGTTATTGCTTCAGTTGACGTGGAGAGACATGCTAGGGATTTTATGGAGGCAGCTAAAAAGCTTCAATTGTACTTCATTGGTTTACAACGTGAGGATCAACCGACCAAAGTGGAAACTTTAAGAAAGGTAAATAATCTCAAGCACCTTTTTAGTTCCAAGCGGGAGTGTCTCTTATGATAAATATGTAAGCAATTCTTTTTTGTAATATTTGTCTTAATTTTCATCAGGAGATATCTGCCATTGAGGAGGAATTAAGATTAAAGAATGACATAATCAAGAAGCAAGAGAGGCTCATTGAAGGGTGGAGAAAGGATTTGAAAGAGCAGTTAGACAAACATAACAATGAGTTAGAGAAGGTTTAG

mRNA sequence

ATGGCTGAACGACAACCACTCGACCAACAGCATCAACTTGATCCACAGTTGCAATCCTCGCAGTCTCCAAGAGAAGATATGATTGCATATGTGATGGCCTTGGAAGCTGCTCTGCTTCCGTGCTTGCCTGCTAGAGAACTACAAGCAATAGACCGTTCTCCTCACCCTTCTCATCAAAGTCAATTTGTCAAGCTTGGGGTTTGTTGGACAAACCATGGGTTTCTAATTCCACTCCCTCCTCATACAATTTTCCCAAATTCAGTTGACGTGGAGAGACATGCTAGGGATTTTATGGAGGCAGCTAAAAAGCTTCAATTGTACTTCATTGGTTTACAACGTGAGGATCAACCGACCAAAGTGGAAACTTTAAGAAAGGAGATATCTGCCATTGAGGAGGAATTAAGATTAAAGAATGACATAATCAAGAAGCAAGAGAGGCTCATTGAAGGGTGGAGAAAGGATTTGAAAGAGCAGTTAGACAAACATAACAATGAGTTAGAGAAGGTTTAG

Coding sequence (CDS)

ATGGCTGAACGACAACCACTCGACCAACAGCATCAACTTGATCCACAGTTGCAATCCTCGCAGTCTCCAAGAGAAGATATGATTGCATATGTGATGGCCTTGGAAGCTGCTCTGCTTCCGTGCTTGCCTGCTAGAGAACTACAAGCAATAGACCGTTCTCCTCACCCTTCTCATCAAAGTCAATTTGTCAAGCTTGGGGTTTGTTGGACAAACCATGGGTTTCTAATTCCACTCCCTCCTCATACAATTTTCCCAAATTCAGTTGACGTGGAGAGACATGCTAGGGATTTTATGGAGGCAGCTAAAAAGCTTCAATTGTACTTCATTGGTTTACAACGTGAGGATCAACCGACCAAAGTGGAAACTTTAAGAAAGGAGATATCTGCCATTGAGGAGGAATTAAGATTAAAGAATGACATAATCAAGAAGCAAGAGAGGCTCATTGAAGGGTGGAGAAAGGATTTGAAAGAGCAGTTAGACAAACATAACAATGAGTTAGAGAAGGTTTAG

Protein sequence

MAERQPLDQQHQLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQSQFVKLGVCWTNHGFLIPLPPHTIFPNSVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELRLKNDIIKKQERLIEGWRKDLKEQLDKHNNELEKV

Homology

[Close]

BLAST of Lag0019674 vs. NCBI nr
Match: XP_038904970.1 (mediator of RNA polymerase II transcription subunit 28 [Benincasa hispida] >XP_038904972.1 mediator of RNA polymerase II transcription subunit 28 [Benincasa hispida] >XP_038904973.1 mediator of RNA polymerase II transcription subunit 28 [Benincasa hispida] >XP_038904974.1 mediator of RNA polymerase II transcription subunit 28 [Benincasa hispida])

HSP 1 Score: 248.1 bits (632), Expect = 5.6e-62
Identity = 135/169 (79.88%), Postives = 139/169 (82.25%), Query Frame = 0


Query: 1   MAERQPLDQQHQLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQS 60
           MAERQPLDQQH LDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQ 
Sbjct: 1   MAERQPLDQQHPLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQ- 60

Query: 61  QFVKLGVCWTNHGFLIPLPPHTIFPNSVDVERHARDFMEAAKKLQLYFIGLQREDQPTKV 120
                                      VDVERHARDFMEAAKKLQLYFIGLQREDQP KV
Sbjct: 61  ---------------------------VDVERHARDFMEAAKKLQLYFIGLQREDQPNKV 120

Query: 121 ETLRKEISAIEEELRLKNDIIKKQERLIEGWRKDLKEQLDKHNNELEKV 170
           ETLRKEISAIEEEL+LKN+IIKKQERLIEGW+KDLK+QLDKHNNELEKV
Sbjct: 121 ETLRKEISAIEEELKLKNEIIKKQERLIEGWKKDLKDQLDKHNNELEKV 141

[Close]

BLAST of Lag0019674 vs. NCBI nr
Match: XP_004152267.1 (mediator of RNA polymerase II transcription subunit 28 [Cucumis sativus] >KGN52780.1 hypothetical protein Csa_014841 [Cucumis sativus])

HSP 1 Score: 247.3 bits (630), Expect = 9.5e-62
Identity = 133/169 (78.70%), Postives = 140/169 (82.84%), Query Frame = 0


Query: 1   MAERQPLDQQHQLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQS 60
           MAERQP+DQQH LDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQ 
Sbjct: 1   MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQ- 60

Query: 61  QFVKLGVCWTNHGFLIPLPPHTIFPNSVDVERHARDFMEAAKKLQLYFIGLQREDQPTKV 120
                                      VDVERHARDFMEAAKKLQLYFIGLQREDQPTKV
Sbjct: 61  ---------------------------VDVERHARDFMEAAKKLQLYFIGLQREDQPTKV 120

Query: 121 ETLRKEISAIEEELRLKNDIIKKQERLIEGWRKDLKEQLDKHNNELEKV 170
           ETLRKEISAIEEEL++KN+IIKKQE+LIEGW+KDLK+QLDKHNNELEKV
Sbjct: 121 ETLRKEISAIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV 141

[Close]

BLAST of Lag0019674 vs. NCBI nr
Match: XP_022922953.1 (mediator of RNA polymerase II transcription subunit 28 [Cucurbita moschata] >XP_022922954.1 mediator of RNA polymerase II transcription subunit 28 [Cucurbita moschata] >XP_022984538.1 mediator of RNA polymerase II transcription subunit 28 [Cucurbita maxima] >XP_022984539.1 mediator of RNA polymerase II transcription subunit 28 [Cucurbita maxima] >XP_023552182.1 mediator of RNA polymerase II transcription subunit 28 [Cucurbita pepo subsp. pepo] >XP_023552183.1 mediator of RNA polymerase II transcription subunit 28 [Cucurbita pepo subsp. pepo] >KAG6576925.1 Mediator of RNA polymerase II transcription subunit 28, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014951.1 Mediator of RNA polymerase II transcription subunit 28 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 246.5 bits (628), Expect = 1.6e-61
Identity = 132/169 (78.11%), Postives = 141/169 (83.43%), Query Frame = 0


Query: 1   MAERQPLDQQHQLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQS 60
           MAERQPLDQQ+Q+DPQLQSSQSPREDM++YVMALEAALLPCLPARELQAIDRSPHPSHQ 
Sbjct: 1   MAERQPLDQQNQIDPQLQSSQSPREDMVSYVMALEAALLPCLPARELQAIDRSPHPSHQ- 60

Query: 61  QFVKLGVCWTNHGFLIPLPPHTIFPNSVDVERHARDFMEAAKKLQLYFIGLQREDQPTKV 120
                                      +DVERHARDFMEAAKKLQLYFIGLQREDQPTKV
Sbjct: 61  ---------------------------IDVERHARDFMEAAKKLQLYFIGLQREDQPTKV 120

Query: 121 ETLRKEISAIEEELRLKNDIIKKQERLIEGWRKDLKEQLDKHNNELEKV 170
           ETLRKEISAIEEEL+LK+DIIKKQERLIEGW+KDLK+QLDKHNNELEKV
Sbjct: 121 ETLRKEISAIEEELKLKDDIIKKQERLIEGWKKDLKDQLDKHNNELEKV 141

[Close]

BLAST of Lag0019674 vs. NCBI nr
Match: XP_008454378.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 28 [Cucumis melo] >XP_008454380.1 PREDICTED: mediator of RNA polymerase II transcription subunit 28 [Cucumis melo] >KAA0044351.1 mediator of RNA polymerase II transcription subunit 28 [Cucumis melo var. makuwa] >TYK29480.1 mediator of RNA polymerase II transcription subunit 28 [Cucumis melo var. makuwa])

HSP 1 Score: 246.5 bits (628), Expect = 1.6e-61
Identity = 135/169 (79.88%), Postives = 138/169 (81.66%), Query Frame = 0


Query: 1   MAERQPLDQQHQLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQS 60
           MAERQ LDQQH LDPQ QSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQ 
Sbjct: 1   MAERQSLDQQHSLDPQSQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQ- 60

Query: 61  QFVKLGVCWTNHGFLIPLPPHTIFPNSVDVERHARDFMEAAKKLQLYFIGLQREDQPTKV 120
                                      VDVERHARDFMEAAKKLQLYFIGLQREDQPTKV
Sbjct: 61  ---------------------------VDVERHARDFMEAAKKLQLYFIGLQREDQPTKV 120

Query: 121 ETLRKEISAIEEELRLKNDIIKKQERLIEGWRKDLKEQLDKHNNELEKV 170
           ETLRKEISAIEEEL++KNDIIKKQERLIEGW+KDLKEQLDKHNNELEKV
Sbjct: 121 ETLRKEISAIEEELKVKNDIIKKQERLIEGWKKDLKEQLDKHNNELEKV 141

[Close]

BLAST of Lag0019674 vs. NCBI nr
Match: XP_022149222.1 (mediator of RNA polymerase II transcription subunit 28 [Momordica charantia])

HSP 1 Score: 242.3 bits (617), Expect = 3.1e-60
Identity = 131/169 (77.51%), Postives = 137/169 (81.07%), Query Frame = 0


Query: 1   MAERQPLDQQHQLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQS 60
           MA+RQP+DQQHQLDPQLQS QSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQ 
Sbjct: 1   MADRQPMDQQHQLDPQLQSPQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQ- 60

Query: 61  QFVKLGVCWTNHGFLIPLPPHTIFPNSVDVERHARDFMEAAKKLQLYFIGLQREDQPTKV 120
                                      +DVERHARDFMEAAKKLQLYFIGLQREDQP KV
Sbjct: 61  ---------------------------IDVERHARDFMEAAKKLQLYFIGLQREDQPIKV 120

Query: 121 ETLRKEISAIEEELRLKNDIIKKQERLIEGWRKDLKEQLDKHNNELEKV 170
           ETLRKEISAIEEEL+LKNDIIKK ERLIEGW+KDLK+QL KHNNELEKV
Sbjct: 121 ETLRKEISAIEEELKLKNDIIKKHERLIEGWKKDLKDQLAKHNNELEKV 141

[Close]

BLAST of Lag0019674 vs. ExPASy Swiss-Prot
Match: Q9LFA5 (Mediator of RNA polymerase II transcription subunit 28 OS=Arabidopsis thaliana OX=3702 GN=MED28 PE=1 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 1.8e-34
Identity = 85/163 (52.15%), Postives = 112/163 (68.71%), Query Frame = 0


Query: 15  PQLQSSQSPR-------EDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQSQFVKLGV 74
           P ++S ++P        ED++A V ALEAALLPCLPARELQAIDRSPHPSHQ        
Sbjct: 22  PGIRSPETPSNNQNNDIEDIMACVTALEAALLPCLPARELQAIDRSPHPSHQ-------- 81

Query: 75  CWTNHGFLIPLPPHTIFPNSVDVERHARDFMEAAKKLQLYFIGLQREDQ-PTKVETLRKE 134
                               +DVERHARDFMEAAKKLQLYF+GL+RED+ P++ E+L+K+
Sbjct: 82  --------------------IDVERHARDFMEAAKKLQLYFMGLKREDRAPSRAESLKKD 141

Query: 135 ISAIEEELRLKNDIIKKQERLIEGWRKDLKEQLDKHNNELEKV 170
           I+ +EEEL+ K+++IKK  RL +  +K +KEQ++KH +ELEKV
Sbjct: 142 IAVMEEELKTKDELIKKHMRLFQESQKLVKEQIEKHRDELEKV 156

The following BLAST results are available for this feature:

BLAST of Lag0019674 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904970.1	5.6e-62	79.88	mediator of RNA polymerase II transcription subunit 28 [Benincasa hispida] >XP_0...	[more]
XP_004152267.1	9.5e-62	78.70	mediator of RNA polymerase II transcription subunit 28 [Cucumis sativus] >KGN527...	[more]
XP_022922953.1	1.6e-61	78.11	mediator of RNA polymerase II transcription subunit 28 [Cucurbita moschata] >XP_...	[more]
XP_008454378.1	1.6e-61	79.88	PREDICTED: mediator of RNA polymerase II transcription subunit 28 [Cucumis melo]...	[more]
XP_022149222.1	3.1e-60	77.51	mediator of RNA polymerase II transcription subunit 28 [Momordica charantia]	[more]

BLAST of Lag0019674 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LFA5	1.8e-34	52.15	Mediator of RNA polymerase II transcription subunit 28 OS=Arabidopsis thaliana O...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 120..169
None	No IPR available	PFAM	PF11594	Med28	coord: 30..153 e-value: 3.5E-14 score: 53.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	PANTHER	PTHR39117:SF1	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 28	coord: 87..169 coord: 1..60
IPR034456	Mediator of RNA polymerase II transcription subunit 28	PANTHER	PTHR39117	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 28	coord: 87..169 coord: 1..60

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0019674.1	Lag0019674.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0016592	mediator complex