Lag0019668 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0019668
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Auxin-responsive GH3 family protein
Location	chr5: 44338831 .. 44340858 (+)
RNA-Seq Expression	Lag0019668
Synteny	Lag0019668

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGTCGATTCTCCTCTCTCTTCCCCTCTCGGCCCTCCCGCCTGTGAAAAGGACGCCAAAGCTCTTCAATTCATCGAACAAATGACTCGCAACGCCCACGCCGTACAGCACACCGTCCTCGCCGAAATCTTGACCAGAAACGCCGCCACCGAGTATCTCCGCCGTTACTCCCTCGCCGGCGCCACCGACCCCCACACCTTCAAAGCTAAACTCCCCGTCATCACCTACGAGGATCTCCAGCCCGAAATCCAACGGATTGCCAGCGGAGATCGCTCGCCTATCCTCTCCTCCCATCCCATCTCCGAGTTCCTCACCAGGTAATATCATTAAATTAACAAATACAAACAACGCATACCGAAGTCTCTCGATCGGAGCAATTGGAATAGTTGAATTGAATTGATTCTTCGATTCTCTGCAGTTCTGGTACTTCTGCCGGTGAAAGGAAGCTTATGCCGACGATCAAGGAGGAGCTGGATCGTCGTCAACTTCTGTACAGTCTTCTCATGCCAGTTATGAACCTGTAAGTGAAAGCAATCCATATCAATGGCGAATTTGAATTATTATTATTTTAAAATTAAAAGCGCGTAAAATTTAATTTGATTATTGAATATTGATTTAATTTATTGGTTTCTGTTTTGCTGTTTGGCTGACAGCTATGTGCCTGGGTTGGATAAAGGCAAGGGGCTTTACTTCTTGTTTGTGAAATCGGAGACGAGGACTCCGGGTGGGCTCTTGGCCCGACCCGTTTTGACCAGCTACTACAAGAGCGAACACTTCAAGACCCGACCCTACGACCCGTTTATGGTCTACACCAGCCCGAATGAGGCCATCCTCTGCCCCGATTCCTTCCAGAGCATGTACACTCAGATGCTCTGCGGTCTTCTCGAACGCCATCAAGTTCTCCGCCTCGGCGCCGTCTTCGCCTCCGGCTTACTCCGGGCCATCAGATTCCTGCAGTTGAACTGGTCCGACCTTGCTCACGATATCCGGACGGGAAAATTGAATTCGAAGATCACCGATCCGTCGTTGAGAGACTGCATCGAGAAGATCCTGAAGCCGGACGCGGAATTAGCGGATTTCGTTTCGGATGAGTGTTCGAAGGAGAATTGGGAGGGAATCGTGACGAGGATTTGGCCGAAAACGAAGTACCTGGACGTGATCGTCACCGGAGCGATGGCGCAGTACATTCCGACGCTGGATTACTACAGCGGCGGATTGCCTTTAGCGTGCACGATGTACGCATCATCGGAGTGTTATTTCGGACTGAATCTGAACCCTATGTGCAAGCCGTCGGAGGTTTCGTACACTATAATGCCAAACATGGCCTATTTCGAGTTCCTCCCTCACGAGCCGAATTCCGGCAGCGATTCGGCGGCGAAGCTCGTCGACCTCGTGGACGTGGAGGTCGGAAAAGAATACGAACTGGTAATCACGACATACGCCGGACTGTACCGGTACCGCGTGGGCGACATACTCCGCGTGACCGGATTCCACAACGCGGCGCCGCAGTTCCACTTCGTGAGGAGGAAGAACGTGGTGCTGAGCATCGACTCGGACAAAACGGACGAGGCGGAGTTGCAGAAGGCGGTGGAGAACGCATCGGAACTGCTGAAGGAATTCAAAGCGAGCGTGGTGGAGTACACGAGTTACGCAGACACGAAGACGATTCCAGGACACTACGTGATATACTGGGAGCTTCTGGTGAAAGAAGCAGAAGGCCGGAAATGGCCGAGCAAGGAGGTGATGGAACAGTGCTGCCTGGCAATGGAGGAATCGCTGAACTCGGTGTACCGGCAAGGAAGAGTTGCAGACAATTCGATCGGAGCTCTGGAAATCAGAGTGGTGAAGAGCGGAACCTTCGAAGAACTGATGGATTACGCAATTTCGAGAGGAGCGTCGATCAACCAGTACAAGGTGCCGAGGTGCGTGAATTTCACTCCCATAATGGAACTTCTCGATTCTAGAGTAGTATCGGCGCATTTTAGTCCTGCTCTGCCGCATTGGACTCCGGCGAGGAGACGCTAA

mRNA sequence

ATGGCTGTCGATTCTCCTCTCTCTTCCCCTCTCGGCCCTCCCGCCTGTGAAAAGGACGCCAAAGCTCTTCAATTCATCGAACAAATGACTCGCAACGCCCACGCCGTACAGCACACCGTCCTCGCCGAAATCTTGACCAGAAACGCCGCCACCGAGTATCTCCGCCGTTACTCCCTCGCCGGCGCCACCGACCCCCACACCTTCAAAGCTAAACTCCCCGTCATCACCTACGAGGATCTCCAGCCCGAAATCCAACGGATTGCCAGCGGAGATCGCTCGCCTATCCTCTCCTCCCATCCCATCTCCGAGTTCCTCACCAGTTCTGGTACTTCTGCCGGTGAAAGGAAGCTTATGCCGACGATCAAGGAGGAGCTGGATCGTCGTCAACTTCTGTACAGTCTTCTCATGCCAGTTATGAACCTCTATGTGCCTGGGTTGGATAAAGGCAAGGGGCTTTACTTCTTGTTTGTGAAATCGGAGACGAGGACTCCGGGTGGGCTCTTGGCCCGACCCGTTTTGACCAGCTACTACAAGAGCGAACACTTCAAGACCCGACCCTACGACCCGTTTATGGTCTACACCAGCCCGAATGAGGCCATCCTCTGCCCCGATTCCTTCCAGAGCATGTACACTCAGATGCTCTGCGGTCTTCTCGAACGCCATCAAGTTCTCCGCCTCGGCGCCGTCTTCGCCTCCGGCTTACTCCGGGCCATCAGATTCCTGCAGTTGAACTGGTCCGACCTTGCTCACGATATCCGGACGGGAAAATTGAATTCGAAGATCACCGATCCGTCGTTGAGAGACTGCATCGAGAAGATCCTGAAGCCGGACGCGGAATTAGCGGATTTCGTTTCGGATGAGTGTTCGAAGGAGAATTGGGAGGGAATCGTGACGAGGATTTGGCCGAAAACGAAGTACCTGGACGTGATCGTCACCGGAGCGATGGCGCAGTACATTCCGACGCTGGATTACTACAGCGGCGGATTGCCTTTAGCGTGCACGATGTACGCATCATCGGAGTGTTATTTCGGACTGAATCTGAACCCTATGTGCAAGCCGTCGGAGGTTTCGTACACTATAATGCCAAACATGGCCTATTTCGAGTTCCTCCCTCACGAGCCGAATTCCGGCAGCGATTCGGCGGCGAAGCTCGTCGACCTCGTGGACGTGGAGGTCGGAAAAGAATACGAACTGGTAATCACGACATACGCCGGACTGTACCGGTACCGCGTGGGCGACATACTCCGCGTGACCGGATTCCACAACGCGGCGCCGCAGTTCCACTTCGTGAGGAGGAAGAACGTGGTGCTGAGCATCGACTCGGACAAAACGGACGAGGCGGAGTTGCAGAAGGCGGTGGAGAACGCATCGGAACTGCTGAAGGAATTCAAAGCGAGCGTGGTGGAGTACACGAGTTACGCAGACACGAAGACGATTCCAGGACACTACGTGATATACTGGGAGCTTCTGGTGAAAGAAGCAGAAGGCCGGAAATGGCCGAGCAAGGAGGTGATGGAACAGTGCTGCCTGGCAATGGAGGAATCGCTGAACTCGGTGTACCGGCAAGGAAGAGTTGCAGACAATTCGATCGGAGCTCTGGAAATCAGAGTGGTGAAGAGCGGAACCTTCGAAGAACTGATGGATTACGCAATTTCGAGAGGAGCGTCGATCAACCAGTACAAGGTGCCGAGGTGCGTGAATTTCACTCCCATAATGGAACTTCTCGATTCTAGAGTAGTATCGGCGCATTTTAGTCCTGCTCTGCCGCATTGGACTCCGGCGAGGAGACGCTAA

Coding sequence (CDS)

ATGGCTGTCGATTCTCCTCTCTCTTCCCCTCTCGGCCCTCCCGCCTGTGAAAAGGACGCCAAAGCTCTTCAATTCATCGAACAAATGACTCGCAACGCCCACGCCGTACAGCACACCGTCCTCGCCGAAATCTTGACCAGAAACGCCGCCACCGAGTATCTCCGCCGTTACTCCCTCGCCGGCGCCACCGACCCCCACACCTTCAAAGCTAAACTCCCCGTCATCACCTACGAGGATCTCCAGCCCGAAATCCAACGGATTGCCAGCGGAGATCGCTCGCCTATCCTCTCCTCCCATCCCATCTCCGAGTTCCTCACCAGTTCTGGTACTTCTGCCGGTGAAAGGAAGCTTATGCCGACGATCAAGGAGGAGCTGGATCGTCGTCAACTTCTGTACAGTCTTCTCATGCCAGTTATGAACCTCTATGTGCCTGGGTTGGATAAAGGCAAGGGGCTTTACTTCTTGTTTGTGAAATCGGAGACGAGGACTCCGGGTGGGCTCTTGGCCCGACCCGTTTTGACCAGCTACTACAAGAGCGAACACTTCAAGACCCGACCCTACGACCCGTTTATGGTCTACACCAGCCCGAATGAGGCCATCCTCTGCCCCGATTCCTTCCAGAGCATGTACACTCAGATGCTCTGCGGTCTTCTCGAACGCCATCAAGTTCTCCGCCTCGGCGCCGTCTTCGCCTCCGGCTTACTCCGGGCCATCAGATTCCTGCAGTTGAACTGGTCCGACCTTGCTCACGATATCCGGACGGGAAAATTGAATTCGAAGATCACCGATCCGTCGTTGAGAGACTGCATCGAGAAGATCCTGAAGCCGGACGCGGAATTAGCGGATTTCGTTTCGGATGAGTGTTCGAAGGAGAATTGGGAGGGAATCGTGACGAGGATTTGGCCGAAAACGAAGTACCTGGACGTGATCGTCACCGGAGCGATGGCGCAGTACATTCCGACGCTGGATTACTACAGCGGCGGATTGCCTTTAGCGTGCACGATGTACGCATCATCGGAGTGTTATTTCGGACTGAATCTGAACCCTATGTGCAAGCCGTCGGAGGTTTCGTACACTATAATGCCAAACATGGCCTATTTCGAGTTCCTCCCTCACGAGCCGAATTCCGGCAGCGATTCGGCGGCGAAGCTCGTCGACCTCGTGGACGTGGAGGTCGGAAAAGAATACGAACTGGTAATCACGACATACGCCGGACTGTACCGGTACCGCGTGGGCGACATACTCCGCGTGACCGGATTCCACAACGCGGCGCCGCAGTTCCACTTCGTGAGGAGGAAGAACGTGGTGCTGAGCATCGACTCGGACAAAACGGACGAGGCGGAGTTGCAGAAGGCGGTGGAGAACGCATCGGAACTGCTGAAGGAATTCAAAGCGAGCGTGGTGGAGTACACGAGTTACGCAGACACGAAGACGATTCCAGGACACTACGTGATATACTGGGAGCTTCTGGTGAAAGAAGCAGAAGGCCGGAAATGGCCGAGCAAGGAGGTGATGGAACAGTGCTGCCTGGCAATGGAGGAATCGCTGAACTCGGTGTACCGGCAAGGAAGAGTTGCAGACAATTCGATCGGAGCTCTGGAAATCAGAGTGGTGAAGAGCGGAACCTTCGAAGAACTGATGGATTACGCAATTTCGAGAGGAGCGTCGATCAACCAGTACAAGGTGCCGAGGTGCGTGAATTTCACTCCCATAATGGAACTTCTCGATTCTAGAGTAGTATCGGCGCATTTTAGTCCTGCTCTGCCGCATTGGACTCCGGCGAGGAGACGCTAA

Protein sequence

MAVDSPLSSPLGPPACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSLAGATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSEHFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRFLQLNWSDLAHDIRTGKLNSKITDPSLRDCIEKILKPDAELADFVSDECSKENWEGIVTRIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNMAYFEFLPHEPNSGSDSAAKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEFKASVVEYTSYADTKTIPGHYVIYWELLVKEAEGRKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR

Homology

[Close]

BLAST of Lag0019668 vs. NCBI nr
Match: XP_022979865.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita maxima])

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 574/597 (96.15%), Postives = 583/597 (97.65%), Query Frame = 0


Query: 1   MAVDSPLSSPLGPPACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSLA 60
           MAVDS LSSPLGPPACEKDAKALQFIEQMTRNA AVQ TVLAEILTRNA+TEYLRRY LA
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP+TFK KLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDP+MVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLAHDIRTGKLNSKITDPSLRDCIEKILKPDAELADFVSDECSKENWEGIVTRI 300
           LQLNWSDLA+DIRTGKL+SKITDPSLRDCIEKILKPD E ADF+S+ECSKENWEGIVTRI
Sbjct: 241 LQLNWSDLANDIRTGKLSSKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGSDSAAKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPNSGSDSA KLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNSGSDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEFKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENAS+LLKE K SVVEYTSYADT TIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480

Query: 481 GHYVIYWELLVKEAEGRKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS 540
           GHYVIYWEL+VKE EG KWPSKE MEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS
Sbjct: 481 GHYVIYWELMVKETEG-KWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS 540

Query: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR 598
           GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR
Sbjct: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR 596

[Close]

BLAST of Lag0019668 vs. NCBI nr
Match: XP_023519906.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita pepo subsp. pepo] >KAG6600299.1 putative indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030958.1 putative indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1150.2 bits (2974), Expect = 0.0e+00
Identity = 573/597 (95.98%), Postives = 582/597 (97.49%), Query Frame = 0


Query: 1   MAVDSPLSSPLGPPACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSLA 60
           MAVDS LSSPLGPPACEKDAKALQFIEQMTRNA AVQ TVLAEILTRNA+TEYLRRY LA
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP+TFK KLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDP+MVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLAHDIRTGKLNSKITDPSLRDCIEKILKPDAELADFVSDECSKENWEGIVTRI 300
           LQLNWSDLA+DIRTGKL+SKITDPSLRDCIEKILKPD E ADF+S+ECSKENWEGIVTRI
Sbjct: 241 LQLNWSDLANDIRTGKLSSKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGSDSAAKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPNSGSDSA KLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNSGSDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEFKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENAS+LLKE K SVVEYTSYADT TIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480

Query: 481 GHYVIYWELLVKEAEGRKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS 540
           GHYVIYWEL+VKE EG KWPSKE MEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS
Sbjct: 481 GHYVIYWELMVKETEG-KWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS 540

Query: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR 598
           GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALP WTPARRR
Sbjct: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 596

[Close]

BLAST of Lag0019668 vs. NCBI nr
Match: XP_022943254.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita moschata])

HSP 1 Score: 1147.9 bits (2968), Expect = 0.0e+00
Identity = 571/597 (95.64%), Postives = 582/597 (97.49%), Query Frame = 0


Query: 1   MAVDSPLSSPLGPPACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSLA 60
           MAVDS LSSPLGPPACEKDAKALQFIEQMTRNA AVQ TVLAEILTRNA+TEYLRRY LA
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP+TFK KLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDP+MVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLAHDIRTGKLNSKITDPSLRDCIEKILKPDAELADFVSDECSKENWEGIVTRI 300
           LQLNWSDLA+DIRTGKL++KITDPSLRDCIEKILKPD E ADF+S+ECSKENWEGIVTRI
Sbjct: 241 LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGSDSAAKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPNSG+DSA KLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEFKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENAS+LLKE K SVVEYTSYADT TIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480

Query: 481 GHYVIYWELLVKEAEGRKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS 540
           GHYVIYWEL+VKE EG KWPSKE MEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS
Sbjct: 481 GHYVIYWELMVKETEG-KWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS 540

Query: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR 598
           GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALP WTPARRR
Sbjct: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 596

[Close]

BLAST of Lag0019668 vs. NCBI nr
Match: XP_022922958.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita moschata])

HSP 1 Score: 1142.1 bits (2953), Expect = 0.0e+00
Identity = 567/597 (94.97%), Postives = 583/597 (97.65%), Query Frame = 0


Query: 1   MAVDSPLSSPLGPPACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSLA 60
           MAVDS LSSPLGPPACEKDAKALQFIEQMTRNA AVQ TVLAEILTRNAATEYLRRYSL 
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPHTFK KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPF+VYTSPNEAILCPDSFQSMYTQMLCGLL+RHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLAHDIRTGKLNSKITDPSLRDCIEKILKPDAELADFVSDECSKENWEGIVTRI 300
           LQLNW DLA+DIR G LNSKITDPSLRDCIEKILKPDAELADFVSDECSKE+WEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGSDSAAKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPN+ SDS  KLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEFKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENAS+LLKE+KASVVEYTSYA+TKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELLVKEAEGRKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS 540
           GHYVIYWEL+VKEAEG KWPSKEVME+CCLAMEESLNSVYRQGRVAD+SIGALEIRVVK+
Sbjct: 481 GHYVIYWELMVKEAEG-KWPSKEVMEECCLAMEESLNSVYRQGRVADHSIGALEIRVVKN 540

Query: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR 598
           GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALP WTPAR R
Sbjct: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596

[Close]

BLAST of Lag0019668 vs. NCBI nr
Match: KAG6576928.1 (putative indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014954.1 putative indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1140.9 bits (2950), Expect = 0.0e+00
Identity = 566/597 (94.81%), Postives = 582/597 (97.49%), Query Frame = 0


Query: 1   MAVDSPLSSPLGPPACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSLA 60
           MAVDS LSSPLGPPACEKDAKALQFIEQMTRNA AVQ TVLAEILTRNAATEYLRRYSL 
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPHTFK KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPF+VYTSPNEAILCPDSFQSMYTQMLCGLL+RHQVLRLGA+FASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRF 240

Query: 241 LQLNWSDLAHDIRTGKLNSKITDPSLRDCIEKILKPDAELADFVSDECSKENWEGIVTRI 300
           LQLNW DLA+DIR G LNSKITDPSLRDCIEKILKPDAELADFVSDECSKE+WEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGSDSAAKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPN+ SDS  KLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEFKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENAS+LLKE+KASVVEYTSYA+TKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELLVKEAEGRKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS 540
           GHYVIYWEL+VKEAEG KWPSKEVME CCLAMEESLNSVYRQGRVAD+SIGALEIRVVK+
Sbjct: 481 GHYVIYWELMVKEAEG-KWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVVKN 540

Query: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR 598
           GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALP WTPAR R
Sbjct: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596

[Close]

BLAST of Lag0019668 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 984.6 bits (2544), Expect = 5.1e-286
Identity = 487/598 (81.44%), Postives = 533/598 (89.13%), Query Frame = 0


Query: 1   MAVDSPLSSPLGPPACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSLA 60
           MAVDS LSSPLGPPACEKDAKAL+FIE+MTRNA  VQ  +LAEIL RNA TEYLRR++L 
Sbjct: 1   MAVDSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLC 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATD  TFK K+PVITYEDLQPEIQRIA GDRSPILS+HPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKG+YFLFVKSET+TPGGL ARPVLTSYYKSE
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HF++RPYDP+ VYTSPNEAILCPDSFQSMYTQMLCGLL+R  VLR+GAVFASGLLRAIRF
Sbjct: 181 HFRSRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLAHDIRTGKLNSKITDPSLRDCIEKILKPDAELADFVSDECSKENWEGIVTRI 300
           LQL+WS  AHDI  G L+S+ITDPS+R C+  ILKPD  LA+F+  EC  +NWE I+TRI
Sbjct: 241 LQLHWSRFAHDIELGCLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WP TKYLDVIVTGAMAQYIPTL+YYSGGLP+ACTMYASSECYFGLNLNPM KPSEVSYTI
Sbjct: 301 WPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGSDSAAKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEF+P           K V+LVDV +GKEYELV+TTYAGL RYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFIPL-------GGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELL-KEFKASVVEYTSYADTKTI 480
           HN+APQFHFVRRKNV+LSIDSDKTDE+ELQKAVENAS +L +E  + V EYTSYADT TI
Sbjct: 421 HNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTI 480

Query: 481 PGHYVIYWELLVKEAEGRKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVK 540
           PGHYV+YWELLV+  +G + PS E + +CCL MEESLNSVYRQ RVADNS+G LEIRVV+
Sbjct: 481 PGHYVLYWELLVR--DGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVR 540

Query: 541 SGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR 598
           +GTFEELMDYAISRGASINQYKVPRCVNFTPI+ELLDSRVVSAHFSP+LPHWTP RRR
Sbjct: 541 NGTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERRR 589

[Close]

BLAST of Lag0019668 vs. ExPASy Swiss-Prot
Match: O22190 (Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=GH3.3 PE=1 SV=1)

HSP 1 Score: 910.2 bits (2351), Expect = 1.2e-263
Identity = 458/599 (76.46%), Postives = 515/599 (85.98%), Query Frame = 0


Query: 1   MAVDSPLSSPLGPPACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSLA 60
           M VDS L SP+      KD KAL+FIE+MTRN   VQ  V+ EIL+RN+ TEYL+R+ L 
Sbjct: 1   MTVDSALRSPMMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLK 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           G TD  TFK K+PV+ Y+DL+PEIQRIA+GDRS ILSS+PI+EFLTSSGTSAGERKLMPT
Sbjct: 61  GFTDRKTFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           I E++DRRQLLYSLLMPVMNLYVPGLDKGK LYFLFVK+E++TPGGL ARPVLTSYYKSE
Sbjct: 121 IDEDMDRRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
            FK RP DP+ VYTSPNEAILCPDS QSMYTQMLCGLL RH+VLRLGAVFASGLLRAI F
Sbjct: 181 QFKRRPNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGF 240

Query: 241 LQLNWSDLAHDIRTGKLNSKITDPSLRDCIEKIL-KPDAELADFVSDECSKEN-WEGIVT 300
           LQ NW +LA DI TG L+S+I+DP++++ + KIL KPD ELADF++  C ++N WEGI+T
Sbjct: 241 LQTNWKELADDISTGTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIIT 300

Query: 301 RIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSY 360
           +IWP TKYLDVIVTGAMAQYIP L+YYSGGLP+ACTMYASSE YFG+NL PMCKPSEVSY
Sbjct: 301 KIWPNTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSY 360

Query: 361 TIMPNMAYFEFLPHEPNSGSDSAAKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVT 420
           TIMPNMAYFEFLPH  +      ++LV+L DVEVGKEYELVITTYAGL RYRVGDIL+VT
Sbjct: 361 TIMPNMAYFEFLPH--HEVPTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVT 420

Query: 421 GFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEFKASVVEYTSYADTKT 480
           GF+N+APQF FVRRKNV+LSI+SDKTDEAELQ AVENAS LL E    V+EYTSYA+TKT
Sbjct: 421 GFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKT 480

Query: 481 IPGHYVIYWELLVKEAEGRKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVV 540
           IPGHYVIYWELLVK+      P+ EVM +CCL MEESLNSVYRQ RVAD SIG LEIRVV
Sbjct: 481 IPGHYVIYWELLVKDQTNP--PNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVV 540

Query: 541 KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR 598
           K+GTFEELMDYAISRGASINQYKVPRCV+FTPIMELLDSRVVS HFSPALPHW+P RRR
Sbjct: 541 KNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERRR 595

[Close]

BLAST of Lag0019668 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 889.4 bits (2297), Expect = 2.2e-257
Identity = 443/591 (74.96%), Postives = 495/591 (83.76%), Query Frame = 0


Query: 7   LSSPLGPPACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSLAGATDPH 66
           L +P      E D + L+FI++MT N  AVQ  VL EIL RNA TEYL +  L GATD  
Sbjct: 14  LRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRA 73

Query: 67  TFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEELD 126
            F+AK+PV++Y+DLQP IQRIA+GDRSPILS+HP+SEFLTSSGTSAGERKLMPTI +ELD
Sbjct: 74  AFRAKVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELD 133

Query: 127 RRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSEHFKTRP 186
           RRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSET+TPGGL ARPVLTSYYKS+HFK RP
Sbjct: 134 RRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRP 193

Query: 187 YDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRFLQLNWS 246
           YDP+  YTSP  AILC D+FQSMY QM+CGL +R+ VLRLGAVFASGLLRAIRFLQLNW 
Sbjct: 194 YDPYHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWE 253

Query: 247 DLAHDIRTGKLNSKITDPSLRDCIEKILKPDAELADFVSDECSKENWEGIVTRIWPKTKY 306
            LA DI +G+L  ++TDPS+R+ +  IL PD ELA  +  ECSK +W GI+TR+WP TKY
Sbjct: 254 QLADDIESGELTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKY 313

Query: 307 LDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNMAY 366
           LDVIVTGAMAQYIPTL++YSGGLP+ACTMYASSECYFGLNL PMC PSEVSYTIMPNM Y
Sbjct: 314 LDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGY 373

Query: 367 FEFLP-HEPNSGSDSAAKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNAAP 426
           FEFLP  E  + S  A +LVDL  VEVG+EYELVITTYAGL RYRVGD+LRVTGFHNAAP
Sbjct: 374 FEFLPVDETGAASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAP 433

Query: 427 QFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEFKASVVEYTSYADTKTIPGHYVI 486
           QF FVRRKNV+LSI+SDKTDEAELQ+AVE AS LL+   ASVVEYTS A TK IPGHYVI
Sbjct: 434 QFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVI 493

Query: 487 YWELLVKEAEGRKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSGTFEE 546
           YWELL K A G      + + +CCL MEE+LN+VYRQ RVAD SIG LEIRVV+ GTFEE
Sbjct: 494 YWELLTKGA-GATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEE 553

Query: 547 LMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARR 597
           LMDYAISRGASINQYKVPRCV F PI+ELLDSRVVS+HFSPALPHWTPARR
Sbjct: 554 LMDYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARR 603

[Close]

BLAST of Lag0019668 vs. ExPASy Swiss-Prot
Match: Q0D4Z6 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.8 PE=1 SV=1)

HSP 1 Score: 889.4 bits (2297), Expect = 2.2e-257
Identity = 443/591 (74.96%), Postives = 495/591 (83.76%), Query Frame = 0


Query: 7   LSSPLGPPACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSLAGATDPH 66
           L +P      E D + L+FI++MT N  AVQ  VL EIL RNA TEYL +  L GATD  
Sbjct: 14  LRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRA 73

Query: 67  TFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEELD 126
            F+AK+PV++Y+DLQP IQRIA+GDRSPILS+HP+SEFLTSSGTSAGERKLMPTI +ELD
Sbjct: 74  AFRAKVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELD 133

Query: 127 RRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSEHFKTRP 186
           RRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSET+TPGGL ARPVLTSYYKS+HFK RP
Sbjct: 134 RRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRP 193

Query: 187 YDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRFLQLNWS 246
           YDP+  YTSP  AILC D+FQSMY QM+CGL +R+ VLRLGAVFASGLLRAIRFLQLNW 
Sbjct: 194 YDPYHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWE 253

Query: 247 DLAHDIRTGKLNSKITDPSLRDCIEKILKPDAELADFVSDECSKENWEGIVTRIWPKTKY 306
            LA DI +G+L  ++TDPS+R+ +  IL PD ELA  +  ECSK +W GI+TR+WP TKY
Sbjct: 254 QLADDIESGELTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKY 313

Query: 307 LDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNMAY 366
           LDVIVTGAMAQYIPTL++YSGGLP+ACTMYASSECYFGLNL PMC PSEVSYTIMPNM Y
Sbjct: 314 LDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGY 373

Query: 367 FEFLP-HEPNSGSDSAAKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNAAP 426
           FEFLP  E  + S  A +LVDL  VEVG+EYELVITTYAGL RYRVGD+LRVTGFHNAAP
Sbjct: 374 FEFLPVDETGAASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAP 433

Query: 427 QFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEFKASVVEYTSYADTKTIPGHYVI 486
           QF FVRRKNV+LSI+SDKTDEAELQ+AVE AS LL+   ASVVEYTS A TK IPGHYVI
Sbjct: 434 QFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVI 493

Query: 487 YWELLVKEAEGRKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSGTFEE 546
           YWELL K A G      + + +CCL MEE+LN+VYRQ RVAD SIG LEIRVV+ GTFEE
Sbjct: 494 YWELLTKGA-GATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEE 553

Query: 547 LMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARR 597
           LMDYAISRGASINQYKVPRCV F PI+ELLDSRVVS+HFSPALPHWTPARR
Sbjct: 554 LMDYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARR 603

[Close]

BLAST of Lag0019668 vs. ExPASy Swiss-Prot
Match: P0C0M2 (Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.2 PE=2 SV=1)

HSP 1 Score: 876.7 bits (2264), Expect = 1.5e-253
Identity = 431/593 (72.68%), Postives = 502/593 (84.65%), Query Frame = 0


Query: 15  ACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSLAGATDPHTFKAKLPV 74
           ACE+DA+ L+FIE+MTR   AVQ  VLA IL RN   EYLRR+ + G TD   FKA++PV
Sbjct: 25  ACERDAEKLEFIEEMTRGFDAVQERVLAAILARNNGAEYLRRHGMEGRTDREAFKARVPV 84

Query: 75  ITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSL 134
           +TYEDL+PEI+RIA+GDRS I+SSHPI+EFLTSSGTSAGERKLMPTI++ELDRRQ+LYSL
Sbjct: 85  VTYEDLRPEIERIANGDRSNIISSHPITEFLTSSGTSAGERKLMPTIEDELDRRQMLYSL 144

Query: 135 LMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSEHFKTRPYDPFMVYT 194
           LMPVMNLYVPGLDKGKGLYFLF+KSET+TPGGL ARPVLTSYYKS+HFK RP+DP+ VYT
Sbjct: 145 LMPVMNLYVPGLDKGKGLYFLFIKSETKTPGGLPARPVLTSYYKSDHFKHRPFDPYNVYT 204

Query: 195 SPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRFLQLNWSDLAHDIRT 254
           SP  AILC D+FQSMY QMLCGL+ R +VLR+GAVFASGLLRAIRFLQL+W +LAHDIRT
Sbjct: 205 SPTAAILCTDAFQSMYAQMLCGLVARAEVLRVGAVFASGLLRAIRFLQLHWRELAHDIRT 264

Query: 255 GKLNSKITDPSLRDCIEKIL-KPDAELADFVSDECSKENWEGIVTRIWPKTKYLDVIVTG 314
           G L++K+T+PS+RD + ++L  PDAELA FV  EC K+ WEGI+TR+WP TKYLDVIVTG
Sbjct: 265 GTLSAKVTEPSIRDAVAEVLAAPDAELAAFVEAECGKDKWEGIITRMWPNTKYLDVIVTG 324

Query: 315 AMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNMAYFEFLPHE 374
           AMAQYIPTL +YSGGLP+ACTMYASSECYFGLNL PMC PSEVSYTIMPNM YFE +PH+
Sbjct: 325 AMAQYIPTLKFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFELMPHD 384

Query: 375 PNS----GSDSAAKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNAAPQFHF 434
           P++          +LVDL D EVG+EYELVITTYAGL RYRVGDIL+VTGFHNAAPQF F
Sbjct: 385 PDAPPLPRDAPPPRLVDLADAEVGREYELVITTYAGLCRYRVGDILQVTGFHNAAPQFRF 444

Query: 435 VRRKNVVLSIDSDKTDEAELQKAVENASELLKEFKASVVEYTSYADTKTIPGHYVIYWEL 494
           VRRKNV+LSIDSDKTDEAELQ AVE AS LL  + AS+VEYTS AD  TIPGHYV+YWEL
Sbjct: 445 VRRKNVLLSIDSDKTDEAELQAAVERASALLSPYGASIVEYTSQADATTIPGHYVVYWEL 504

Query: 495 LVKEAEGRKWP------SKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSGTF 554
           +V+  EG  WP       + V E+CCL MEE+LN+VYRQGR  + +IG LEIRVV++GTF
Sbjct: 505 MVR--EGGAWPPPAEEEGRGVFERCCLEMEEALNAVYRQGRNGE-AIGPLEIRVVRAGTF 564

Query: 555 EELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARR 597
           EE+MDYAISRGASINQYK PRCV+F PI+ELL+SRV+S HFSPA P ++P ++
Sbjct: 565 EEVMDYAISRGASINQYKAPRCVSFGPIIELLNSRVISKHFSPACPKYSPHKK 614

[Close]

BLAST of Lag0019668 vs. ExPASy TrEMBL
Match: A0A6J1IUJ6 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661 GN=LOC111479427 PE=3 SV=1)

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 574/597 (96.15%), Postives = 583/597 (97.65%), Query Frame = 0


Query: 1   MAVDSPLSSPLGPPACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSLA 60
           MAVDS LSSPLGPPACEKDAKALQFIEQMTRNA AVQ TVLAEILTRNA+TEYLRRY LA
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP+TFK KLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDP+MVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLAHDIRTGKLNSKITDPSLRDCIEKILKPDAELADFVSDECSKENWEGIVTRI 300
           LQLNWSDLA+DIRTGKL+SKITDPSLRDCIEKILKPD E ADF+S+ECSKENWEGIVTRI
Sbjct: 241 LQLNWSDLANDIRTGKLSSKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGSDSAAKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPNSGSDSA KLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNSGSDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEFKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENAS+LLKE K SVVEYTSYADT TIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480

Query: 481 GHYVIYWELLVKEAEGRKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS 540
           GHYVIYWEL+VKE EG KWPSKE MEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS
Sbjct: 481 GHYVIYWELMVKETEG-KWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS 540

Query: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR 598
           GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR
Sbjct: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR 596

[Close]

BLAST of Lag0019668 vs. ExPASy TrEMBL
Match: A0A6J1FTR9 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=3662 GN=LOC111448040 PE=3 SV=1)

HSP 1 Score: 1147.9 bits (2968), Expect = 0.0e+00
Identity = 571/597 (95.64%), Postives = 582/597 (97.49%), Query Frame = 0


Query: 1   MAVDSPLSSPLGPPACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSLA 60
           MAVDS LSSPLGPPACEKDAKALQFIEQMTRNA AVQ TVLAEILTRNA+TEYLRRY LA
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP+TFK KLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDP+MVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLAHDIRTGKLNSKITDPSLRDCIEKILKPDAELADFVSDECSKENWEGIVTRI 300
           LQLNWSDLA+DIRTGKL++KITDPSLRDCIEKILKPD E ADF+S+ECSKENWEGIVTRI
Sbjct: 241 LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGSDSAAKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPNSG+DSA KLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEFKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENAS+LLKE K SVVEYTSYADT TIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480

Query: 481 GHYVIYWELLVKEAEGRKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS 540
           GHYVIYWEL+VKE EG KWPSKE MEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS
Sbjct: 481 GHYVIYWELMVKETEG-KWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS 540

Query: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR 598
           GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALP WTPARRR
Sbjct: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 596

[Close]

BLAST of Lag0019668 vs. ExPASy TrEMBL
Match: A0A6J1E8A0 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=3662 GN=LOC111430783 PE=3 SV=1)

HSP 1 Score: 1142.1 bits (2953), Expect = 0.0e+00
Identity = 567/597 (94.97%), Postives = 583/597 (97.65%), Query Frame = 0


Query: 1   MAVDSPLSSPLGPPACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSLA 60
           MAVDS LSSPLGPPACEKDAKALQFIEQMTRNA AVQ TVLAEILTRNAATEYLRRYSL 
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPHTFK KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPF+VYTSPNEAILCPDSFQSMYTQMLCGLL+RHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLAHDIRTGKLNSKITDPSLRDCIEKILKPDAELADFVSDECSKENWEGIVTRI 300
           LQLNW DLA+DIR G LNSKITDPSLRDCIEKILKPDAELADFVSDECSKE+WEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGSDSAAKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPN+ SDS  KLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEFKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENAS+LLKE+KASVVEYTSYA+TKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELLVKEAEGRKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS 540
           GHYVIYWEL+VKEAEG KWPSKEVME+CCLAMEESLNSVYRQGRVAD+SIGALEIRVVK+
Sbjct: 481 GHYVIYWELMVKEAEG-KWPSKEVMEECCLAMEESLNSVYRQGRVADHSIGALEIRVVKN 540

Query: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR 598
           GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALP WTPAR R
Sbjct: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596

[Close]

BLAST of Lag0019668 vs. ExPASy TrEMBL
Match: A0A6J1J7V1 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661 GN=LOC111483372 PE=3 SV=1)

HSP 1 Score: 1139.4 bits (2946), Expect = 0.0e+00
Identity = 564/597 (94.47%), Postives = 582/597 (97.49%), Query Frame = 0


Query: 1   MAVDSPLSSPLGPPACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSLA 60
           MAVDS LSSPLGPPACEKDAKALQFIEQMTRNA AVQ TVLAEILTRNAATEYLRRYSL 
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           G TDPHTFK KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GTTDPHTFKGKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPF+VYTSPNEAILCPDSFQSMYTQMLCGLL+RHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLAHDIRTGKLNSKITDPSLRDCIEKILKPDAELADFVSDECSKENWEGIVTRI 300
           LQLNW DLA+DIR G LN+KITDPSLRDCIEKILKPDAELADFVSDECSKE+WEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNTKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGSDSAAKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPN+ SDS  KLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEFKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENAS+LLKE+KASVVEYTSYA+TKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELLVKEAEGRKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS 540
           GHYVIYWEL+VKEAEG KWPSKEVME+CCLAMEESLNSVYRQGRVAD+SIGALEIRVVK+
Sbjct: 481 GHYVIYWELMVKEAEG-KWPSKEVMEECCLAMEESLNSVYRQGRVADHSIGALEIRVVKN 540

Query: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR 598
           GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRV+SAHFSPALP WTPAR R
Sbjct: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVISAHFSPALPRWTPARTR 596

[Close]

BLAST of Lag0019668 vs. ExPASy TrEMBL
Match: A0A5A7T148 (Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold379G001170 PE=3 SV=1)

HSP 1 Score: 1121.3 bits (2899), Expect = 0.0e+00
Identity = 558/600 (93.00%), Postives = 580/600 (96.67%), Query Frame = 0


Query: 1   MAVDSPLSSPLGPPACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSLA 60
           MAV S LSSPLGPPACE DAK LQFI++MTRNA AVQHTVLAEIL+RNA+TEYLRRYSL 
Sbjct: 1   MAVHSSLSSPLGPPACETDAKPLQFIDEMTRNADAVQHTVLAEILSRNASTEYLRRYSLD 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP TFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLL+R+QVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLAHDIRTGKLNSKITDPSLRDCIEKILKPDAELADFVSDECSKENWEGIVTRI 300
           LQLNW DLA DIR G LNS+ITDPSLRDCI KILKPD++LADFVSDECSKE+WEGIVTRI
Sbjct: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCILKILKPDSQLADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLD+YSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGSDSAAKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLP EPNSGSDS+ KLVDLVDVE+GKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPLEPNSGSDSSPKLVDLVDVEIGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEFKASVVEYTSYADTKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKE+KASVVEYTSYA+TKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELLVKEA---EGR-KWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIR 540
           GHYVIYWEL+VKEA   EG+ KWPSKEVME+CCL MEES+NSVYRQGRVAD+SIGALEIR
Sbjct: 481 GHYVIYWELMVKEAGDQEGKGKWPSKEVMEECCLRMEESMNSVYRQGRVADHSIGALEIR 540

Query: 541 VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARR 597
           VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVS HFSPALPHWTPAR+
Sbjct: 541 VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPARK 600

[Close]

BLAST of Lag0019668 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 984.6 bits (2544), Expect = 3.6e-287
Identity = 487/598 (81.44%), Postives = 533/598 (89.13%), Query Frame = 0


Query: 1   MAVDSPLSSPLGPPACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSLA 60
           MAVDS LSSPLGPPACEKDAKAL+FIE+MTRNA  VQ  +LAEIL RNA TEYLRR++L 
Sbjct: 1   MAVDSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLC 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATD  TFK K+PVITYEDLQPEIQRIA GDRSPILS+HPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKG+YFLFVKSET+TPGGL ARPVLTSYYKSE
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HF++RPYDP+ VYTSPNEAILCPDSFQSMYTQMLCGLL+R  VLR+GAVFASGLLRAIRF
Sbjct: 181 HFRSRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLAHDIRTGKLNSKITDPSLRDCIEKILKPDAELADFVSDECSKENWEGIVTRI 300
           LQL+WS  AHDI  G L+S+ITDPS+R C+  ILKPD  LA+F+  EC  +NWE I+TRI
Sbjct: 241 LQLHWSRFAHDIELGCLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WP TKYLDVIVTGAMAQYIPTL+YYSGGLP+ACTMYASSECYFGLNLNPM KPSEVSYTI
Sbjct: 301 WPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGSDSAAKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEF+P           K V+LVDV +GKEYELV+TTYAGL RYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFIPL-------GGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELL-KEFKASVVEYTSYADTKTI 480
           HN+APQFHFVRRKNV+LSIDSDKTDE+ELQKAVENAS +L +E  + V EYTSYADT TI
Sbjct: 421 HNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTI 480

Query: 481 PGHYVIYWELLVKEAEGRKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVK 540
           PGHYV+YWELLV+  +G + PS E + +CCL MEESLNSVYRQ RVADNS+G LEIRVV+
Sbjct: 481 PGHYVLYWELLVR--DGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVR 540

Query: 541 SGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR 598
           +GTFEELMDYAISRGASINQYKVPRCVNFTPI+ELLDSRVVSAHFSP+LPHWTP RRR
Sbjct: 541 NGTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERRR 589

[Close]

BLAST of Lag0019668 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 928.3 bits (2398), Expect = 3.1e-270
Identity = 467/606 (77.06%), Postives = 524/606 (86.47%), Query Frame = 0


Query: 1   MAVDSPLSSPL-GPPACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSL 60
           MAVDSPL S +      EKD KAL+FIE+MTRN  +VQ  VL EILTRN+ TEYL+R+ L
Sbjct: 1   MAVDSPLQSRMVSATTSEKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDL 60

Query: 61  AGATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMP 120
            G  D  TFK+K+PV+TYEDL+PEIQRI++GD SPILSSHPI+EFLTSSGTSAGERKLMP
Sbjct: 61  DGVVDRKTFKSKVPVVTYEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMP 120

Query: 121 TIKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS 180
           TI+E+LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSE++T GGL ARPVLTSYYKS
Sbjct: 121 TIEEDLDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKS 180

Query: 181 EHFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIR 240
           +HFK RPYDP+ VYTSPNEAILC DS QSMY QMLCGLL RH+VLRLGAVFASGLLRAI 
Sbjct: 181 DHFKRRPYDPYNVYTSPNEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAIS 240

Query: 241 FLQLNWSDLAHDIRTGKLNSKITDPSLRDCIEKIL-KPDAELADFVSDECSKENWEGIVT 300
           FLQ NW +LA DI TG L+S+I DP++++ + KIL KPD ELA+F+   CS+ENWEGI+T
Sbjct: 241 FLQNNWKELARDISTGTLSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIIT 300

Query: 301 RIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSY 360
           +IWP TKYLDVIVTGAMAQYIPTL+YYSGGLP+ACTMYASSE YFG+NL PMCKPSEVSY
Sbjct: 301 KIWPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSY 360

Query: 361 TIMPNMAYFEFLPHEPNSGSDSAAK-------LVDLVDVEVGKEYELVITTYAGLYRYRV 420
           TIMPNMAYFEFLPH  N   D AA+       LV+L +VEVGKEYELVITTYAGLYRYRV
Sbjct: 361 TIMPNMAYFEFLPH--NHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRV 420

Query: 421 GDILRVTGFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEFKASVVEYT 480
           GDILRVTGFHN+APQF F+RRKNV+LS++SDKTDEAELQKAVENAS L  E    V+EYT
Sbjct: 421 GDILRVTGFHNSAPQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYT 480

Query: 481 SYADTKTIPGHYVIYWELLVKEAEGRKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIG 540
           SYA+TKTIPGHYVIYWELL ++ +     S+EVM +CCL MEESLNSVYRQ RVAD SIG
Sbjct: 481 SYAETKTIPGHYVIYWELLGRD-QSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIG 540

Query: 541 ALEIRVVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHW 598
            LEIRVV++GTFEELMDYAISRGASINQYKVPRCV+FTPIMELLDSRVVSAHFSP+LPHW
Sbjct: 541 PLEIRVVRNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHW 600

[Close]

BLAST of Lag0019668 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 910.2 bits (2351), Expect = 8.6e-265
Identity = 458/599 (76.46%), Postives = 515/599 (85.98%), Query Frame = 0


Query: 1   MAVDSPLSSPLGPPACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSLA 60
           M VDS L SP+      KD KAL+FIE+MTRN   VQ  V+ EIL+RN+ TEYL+R+ L 
Sbjct: 1   MTVDSALRSPMMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLK 60

Query: 61  GATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           G TD  TFK K+PV+ Y+DL+PEIQRIA+GDRS ILSS+PI+EFLTSSGTSAGERKLMPT
Sbjct: 61  GFTDRKTFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           I E++DRRQLLYSLLMPVMNLYVPGLDKGK LYFLFVK+E++TPGGL ARPVLTSYYKSE
Sbjct: 121 IDEDMDRRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
            FK RP DP+ VYTSPNEAILCPDS QSMYTQMLCGLL RH+VLRLGAVFASGLLRAI F
Sbjct: 181 QFKRRPNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGF 240

Query: 241 LQLNWSDLAHDIRTGKLNSKITDPSLRDCIEKIL-KPDAELADFVSDECSKEN-WEGIVT 300
           LQ NW +LA DI TG L+S+I+DP++++ + KIL KPD ELADF++  C ++N WEGI+T
Sbjct: 241 LQTNWKELADDISTGTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIIT 300

Query: 301 RIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSY 360
           +IWP TKYLDVIVTGAMAQYIP L+YYSGGLP+ACTMYASSE YFG+NL PMCKPSEVSY
Sbjct: 301 KIWPNTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSY 360

Query: 361 TIMPNMAYFEFLPHEPNSGSDSAAKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVT 420
           TIMPNMAYFEFLPH  +      ++LV+L DVEVGKEYELVITTYAGL RYRVGDIL+VT
Sbjct: 361 TIMPNMAYFEFLPH--HEVPTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVT 420

Query: 421 GFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEFKASVVEYTSYADTKT 480
           GF+N+APQF FVRRKNV+LSI+SDKTDEAELQ AVENAS LL E    V+EYTSYA+TKT
Sbjct: 421 GFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKT 480

Query: 481 IPGHYVIYWELLVKEAEGRKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVV 540
           IPGHYVIYWELLVK+      P+ EVM +CCL MEESLNSVYRQ RVAD SIG LEIRVV
Sbjct: 481 IPGHYVIYWELLVKDQTNP--PNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVV 540

Query: 541 KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR 598
           K+GTFEELMDYAISRGASINQYKVPRCV+FTPIMELLDSRVVS HFSPALPHW+P RRR
Sbjct: 541 KNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERRR 595

[Close]

BLAST of Lag0019668 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 861.7 bits (2225), Expect = 3.5e-250
Identity = 435/599 (72.62%), Postives = 504/599 (84.14%), Query Frame = 0


Query: 1   MAVDSPLSSPL-GPPACEKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSL 60
           MAVDS L S +  P   E + KAL+FIE++TRN  +VQ  VL EIL+RN+ TEYL+R+ L
Sbjct: 1   MAVDSLLQSGMASPTTSETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDL 60

Query: 61  AGATDPHTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMP 120
            GA D  +FK+K+PV+ YEDL+ +IQRI++GDRSPILSSHPI+EFLTSSGTSAGERKLMP
Sbjct: 61  NGAVDRKSFKSKVPVVIYEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMP 120

Query: 121 TIKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS 180
           TI+E+++RRQLL +LLMPVMNLYVPGLDKGKGLYFLFVKSE+ T GGL ARP LTSYYKS
Sbjct: 121 TIEEDINRRQLLGNLLMPVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKS 180

Query: 181 EHFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIR 240
           ++F+T   D   VYTSP EAILC DS QSMYTQMLCGLL RH+V RLGAVF SGLLRAI 
Sbjct: 181 DYFRTSDSD--SVYTSPKEAILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAIS 240

Query: 241 FLQLNWSDLAHDIRTGKLNSKITDPSLRDCIEKIL-KPDAELADFVSDECSKENWEGIVT 300
           FLQ NW +L+ DI TG L+SKI D +++  +  IL KPD ELA+F+   CS+ENWEGI+T
Sbjct: 241 FLQNNWKELSQDISTGTLSSKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIIT 300

Query: 301 RIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSY 360
           +IWP TKYLDVIVTGAMA+YIP L+YYSGGLP+A  +YASSE YFG+NLNPMCKPSEVSY
Sbjct: 301 KIWPNTKYLDVIVTGAMAEYIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSY 360

Query: 361 TIMPNMAYFEFLPH-EPNSGSDSAAKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRV 420
           TI PNMAYFEFLPH     G   A  LV+L DVEVGKEYELVITTYAGLYRYRVGDILRV
Sbjct: 361 TIFPNMAYFEFLPHNHDGDGGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRV 420

Query: 421 TGFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEFKASVVEYTSYADTK 480
           TGFHN+APQF F+RR+NV+LSI+SDKTDEA+LQKAVENAS LL E    V+EYTSYADTK
Sbjct: 421 TGFHNSAPQFKFIRRENVLLSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTK 480

Query: 481 TIPGHYVIYWELLVKEAEGRKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRV 540
           TIPGHYVIYWELL ++ +    PS EVM +CCL MEESLN+VYRQ RV+D SIG LEIRV
Sbjct: 481 TIPGHYVIYWELLSRD-QSNALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRV 540

Query: 541 VKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARR 597
           V++GTFEELMD++ISRG+SINQYKVPRCV+ TPIM+LLDSRVVSAHFSP+LPHW+P RR
Sbjct: 541 VQNGTFEELMDFSISRGSSINQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPERR 596

[Close]

BLAST of Lag0019668 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 824.7 bits (2129), Expect = 4.8e-239
Identity = 403/593 (67.96%), Postives = 479/593 (80.78%), Query Frame = 0


Query: 17  EKDAKALQFIEQMTRNAHAVQHTVLAEILTRNAATEYLRRYSLAGATDPHTFKAKLPVIT 76
           EK+   LQFIE +T NA  VQ  VL EIL+RNA  EYL+R+ L G TD  TFK  +PV+T
Sbjct: 19  EKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVT 78

Query: 77  YEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136
           YED+QPEI RIA+GD+S +L S+PISEFLTSSGTS GERKLMPTI+EELDRR LLYSLLM
Sbjct: 79  YEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLM 138

Query: 137 PVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSEHFKTRPYDPFMVYTSP 196
           PVM+ +VPGLDKGKG+YFLF+KSE++TPGGL ARPVLTSYYKS HFK RPYDP+  YTSP
Sbjct: 139 PVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSP 198

Query: 197 NEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRFLQLNWSDLAHDIRTGK 256
           N+ ILC DS+QSMY+QMLCGL +  +VLR+GAVFASG +RAI+FL+ +W +LA DIRTG 
Sbjct: 199 NQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGT 258

Query: 257 LNSKITDPSLRDCIEKILKPDAELADFVSDECSKENWEGIVTRIWPKTKYLDVIVTGAMA 316
           L+S+ITD S+R+ + +ILKPD +LADFV  EC K +W+GI+TR+WP TKY+DVIVTG M+
Sbjct: 259 LSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMS 318

Query: 317 QYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNMAYFEFLPHEPNS 376
           QYIPTLDYYS GLPL CTMYASSECYFG+NL P+CKPSEVSYT++PNMAYFEFLP   NS
Sbjct: 319 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNS 378

Query: 377 GSDSA------------AKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNAA 436
           G  S+             +LVDLVDV++G+EYELV+TTYAGLYRYRVGD+L V GF N A
Sbjct: 379 GVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNA 438

Query: 437 PQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEFKASVVEYTSYADTKTIPGHYV 496
           PQF F+ RKNVVLSIDSDKTDE ELQ AV+NA   L  F AS+ EYTSYADT +IPGHYV
Sbjct: 439 PQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYV 498

Query: 497 IYWELLVKEAEGRKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSGTFE 556
           ++WEL +    G       V E CCL +EESLNSVYRQGRV+D SIG LEI++V+SGTF+
Sbjct: 499 LFWELCL---NGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFD 558

Query: 557 ELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR 598
           +LMDYAIS GASINQYK PRCV F PI+ELL+SRVV ++FSP  P W+P  ++
Sbjct: 559 KLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHKQ 608

The following BLAST results are available for this feature:

BLAST of Lag0019668 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022979865.1	0.0e+00	96.15	probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita maxima]	[more]
XP_023519906.1	0.0e+00	95.98	probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita pepo subsp. pepo...	[more]
XP_022943254.1	0.0e+00	95.64	probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita moschata]	[more]
XP_022922958.1	0.0e+00	94.97	probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita moschata]	[more]
KAG6576928.1	0.0e+00	94.81	putative indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma ...	[more]

BLAST of Lag0019668 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82333	5.1e-286	81.44	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=...	[more]
O22190	1.2e-263	76.46	Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
A3BLS0	2.2e-257	74.96	Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399...	[more]
Q0D4Z6	2.2e-257	74.96	Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=3...	[more]
P0C0M2	1.5e-253	72.68	Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japo...	[more]

BLAST of Lag0019668 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IUJ6	0.0e+00	96.15	probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661...	[more]
A0A6J1FTR9	0.0e+00	95.64	probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=36...	[more]
A0A6J1E8A0	0.0e+00	94.97	probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=36...	[more]
A0A6J1J7V1	0.0e+00	94.47	probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661...	[more]
A0A5A7T148	0.0e+00	93.00	Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa...	[more]

BLAST of Lag0019668 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G14960.1	3.6e-287	81.44	Auxin-responsive GH3 family protein	[more]
AT4G37390.1	3.1e-270	77.06	Auxin-responsive GH3 family protein	[more]
AT2G23170.1	8.6e-265	76.46	Auxin-responsive GH3 family protein	[more]
AT1G59500.1	3.5e-250	72.62	Auxin-responsive GH3 family protein	[more]
AT5G54510.1	4.8e-239	67.96	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 446..466
None	No IPR available	PANTHER	PTHR31901:SF7	INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.2-RELATED	coord: 5..597
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 26..568 e-value: 1.7E-205 score: 683.9
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 5..597

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0019668.1	Lag0019668.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016881	acid-amino acid ligase activity