Lag0019605 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0019605
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	basic blue protein-like
Location	chr5: 43743754 .. 43745776 (-)
RNA-Seq Expression	Lag0019605
Synteny	Lag0019605

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTGGGGAAGGGGCAGTGCAGTGTTGGGTTGCTTGTTGTTGGTTCTTCAATACTCTGAAATGGTTCGTGCAGCTGTTTACACTGTTGGAGATGATCAAGGCTGGACCTTCAACGTCGACTCCTGGCCCAAGCAAAAGCTCTTCCGTGCTGGCGACACACTTGGTAACTTTGGCTCCGTTTGATAACCATTTCGTTTTTAGTTTTTGGTTTTTGAAAATTATGTTTGTTTTCTTTCAATCTACTTACCATGGTTTTCATTTTGTTTAGTAGACATTTGAATTCCTAGCCAAATTTCAAAAACAAAAACAAGTTTTTGAAAACTTTTTTTTTTTTTTCAAATTTTGGCTTGTTTTTTGAAAACATGGGAAATGATATAGATAACAAACAAAGAAACTTGTGGGTAAAAAAAATGTTTATAAGCTTAATTTAAAAAAAAAAAACAAAAAACGAAATAGTTATTAAATGGGGCCTTCATTTTTCACTTTTCTTTCTCGATCTCAGAAAAAATTTCTAGTACCAAGGAAGTATACGCACTCTCTTTGTTCACACACTCTGTTTTTGTCTTTATAAGTAATATTTGAATGTTGGTGGACTCAACGTTGTCGTTTTTAGAATGATGCTGATGTGGCAAGAAATGAGTGAAAGGAGTATTGTATCCCTACTCTCTCATGGTATAAGAATTATAAGTCTGGTTTTCTTGGATTTAAAGAGTCTAATTAATCTCTAAACTTTTAATATTGTGTTATGACTGATAGGTTGTTGAAACTTAAAAAAGTATGTAATAAGTTTTGTGAACACAATTAATTTTGTATTTAGTAGGTTTTTGAATTTTAAACAACGTCACACATAAATTAAGAATTATTAAATGTACGTAAAATTGAGAGTAATTTAGAGATGATATCAGCGATAAAATTAAAAGTTTGTGGATTTATTAAATATATATATTTTTAAATAATATATATGCATGGATCTTTTAGATATAATTGTGTTAGGCACACTTTTTAAATTTAGAAATGTGTTGGACACAACTGAAAAAGCTTAGGGACCACAATTATCATTTAGCCCTAGTTTCTAGGGATGGGATTTAGAAGGATGATATAAATGAAACATTTGAAAGAAATATGTATAAAATAAAAAAGATTTTTAGTTTGTATTTAACATCTTTTAAAAAAAGGTATTTTGAAGTGAAACACTTGATATATGGAAGTGCTTTAGGAAGAAAAATAATTAAACAATATACTTCTTCTAAAAGTTCTTTAACTCTTGTGTGTGTATGTTTTTTTAATTACTCCACCACTTTTGGTTATTGAAATTATTTAAATAAAAATTTTAATGAGCCAAAAATACTTTTTGCCTCTCTTTTAAATTTTGACCAATAACCTATAAACTTGTTGGTTTGAGTTCTGTAAAGCAAATCCATGTAGCTTATAAATGGAAGGAACATACTCAAATAATATATTTTGAGGTTAAGAACAATCAATCCAAACTACCTTTCAACCAAGTTGGCATATTTAAAATAACAATAATTCATCATAAAAACACAAACATATATATATATTATTTAAGGCCACACTTGCCCATGCATCTTATTATACATATAAGTATAAAATTTTAAAAAAGAAATTGGGAATTCCACTATATACATATATTATAATTAAGTCCACACACGAACCTCTGATAAGATAAATCATGTCTAGATCAGCTAAGCTATGTTCATATATCGACATAAACAACTCTAGAGTTAAATACATCCTAGTTTAACGAGTTTATTTTCATAACATAAAGTACTTTTCGTTAAAAACTCATGTGCTTGTTACAGTGTTCAACTATAGTCCTGAGACACACAATGTTGTGGCTGTGAACCGAGTGGGGTACAATCGGTGCATAAGTCCAAGGGGTTCGAAAATTTTTCAAACAGGACAAGATCAAATCAAGCTTGTGAAGGGACAAAACTTCTTCATCTGCAGCCTCCCAGGCCACTGCCAAGGTGGAATGAAGATTGCCATTACTGCACTTTAA

mRNA sequence

ATGGGTTGGGGAAGGGGCAGTGCAGTGTTGGGTTGCTTGTTGTTGGTTCTTCAATACTCTGAAATGGTTCGTGCAGCTGTTTACACTGTTGGAGATGATCAAGGCTGGACCTTCAACGTCGACTCCTGGCCCAAGCAAAAGCTCTTCCGTGCTGGCGACACACTTGTGTTCAACTATAGTCCTGAGACACACAATGTTGTGGCTGTGAACCGAGTGGGGTACAATCGGTGCATAAGTCCAAGGGGTTCGAAAATTTTTCAAACAGGACAAGATCAAATCAAGCTTGTGAAGGGACAAAACTTCTTCATCTGCAGCCTCCCAGGCCACTGCCAAGGTGGAATGAAGATTGCCATTACTGCACTTTAA

Coding sequence (CDS)

ATGGGTTGGGGAAGGGGCAGTGCAGTGTTGGGTTGCTTGTTGTTGGTTCTTCAATACTCTGAAATGGTTCGTGCAGCTGTTTACACTGTTGGAGATGATCAAGGCTGGACCTTCAACGTCGACTCCTGGCCCAAGCAAAAGCTCTTCCGTGCTGGCGACACACTTGTGTTCAACTATAGTCCTGAGACACACAATGTTGTGGCTGTGAACCGAGTGGGGTACAATCGGTGCATAAGTCCAAGGGGTTCGAAAATTTTTCAAACAGGACAAGATCAAATCAAGCTTGTGAAGGGACAAAACTTCTTCATCTGCAGCCTCCCAGGCCACTGCCAAGGTGGAATGAAGATTGCCATTACTGCACTTTAA

Protein sequence

MGWGRGSAVLGCLLLVLQYSEMVRAAVYTVGDDQGWTFNVDSWPKQKLFRAGDTLVFNYSPETHNVVAVNRVGYNRCISPRGSKIFQTGQDQIKLVKGQNFFICSLPGHCQGGMKIAITAL

Homology

[Close]

BLAST of Lag0019605 vs. NCBI nr
Match: XP_038878143.1 (basic blue protein-like [Benincasa hispida])

HSP 1 Score: 212.6 bits (540), Expect = 1.9e-51
Identity = 101/125 (80.80%), Postives = 109/125 (87.20%), Query Frame = 0


Query: 1   MGWGRGSA----VLGCLLLVLQYSEMVRAAVYTVGDDQGWTFNVDSWPKQKLFRAGDTLV 60
           MG GRGSA    V+ CLLL LQY EM  AAVYTVGDDQGWTFNVDSW  QKLFRAGDTLV
Sbjct: 1   MGEGRGSAAVSIVMACLLL-LQYCEMAHAAVYTVGDDQGWTFNVDSWTNQKLFRAGDTLV 60

Query: 61  FNYSPETHNVVAVNRVGYNRCISPRGSKIFQTGQDQIKLVKGQNFFICSLPGHCQGGMKI 120
           FNY P  HNVVAVN++GYNRCI+PRGSK+FQTG+DQIKLVKGQNFFIC+LPGHCQ GMKI
Sbjct: 61  FNYRPVAHNVVAVNKMGYNRCITPRGSKVFQTGKDQIKLVKGQNFFICNLPGHCQTGMKI 120

Query: 121 AITAL 122
           AITA+
Sbjct: 121 AITAI 124

[Close]

BLAST of Lag0019605 vs. NCBI nr
Match: XP_038903112.1 (basic blue protein-like [Benincasa hispida])

HSP 1 Score: 209.5 bits (532), Expect = 1.6e-50
Identity = 98/125 (78.40%), Postives = 108/125 (86.40%), Query Frame = 0


Query: 1   MGWGRGSA----VLGCLLLVLQYSEMVRAAVYTVGDDQGWTFNVDSWPKQKLFRAGDTLV 60
           MG GRGSA    V+ C+LL++QYS+M  AAVY VG  QGWTFNV SWPK K FRAGDTLV
Sbjct: 1   MGEGRGSAVVCVVMVCVLLMVQYSQMAHAAVYPVGGAQGWTFNVASWPKGKRFRAGDTLV 60

Query: 61  FNYSPETHNVVAVNRVGYNRCISPRGSKIFQTGQDQIKLVKGQNFFICSLPGHCQGGMKI 120
           FNYSP  HNVVAVNRVGYNRCI+PRGSK+FQTG+DQIKLVKGQNFFIC+LPGHCQ GMKI
Sbjct: 61  FNYSPSAHNVVAVNRVGYNRCITPRGSKVFQTGKDQIKLVKGQNFFICNLPGHCQSGMKI 120

Query: 121 AITAL 122
           AITA+
Sbjct: 121 AITAI 125

[Close]

BLAST of Lag0019605 vs. NCBI nr
Match: XP_008465594.1 (PREDICTED: basic blue protein-like [Cucumis melo] >KAA0039078.1 basic blue protein-like [Cucumis melo var. makuwa] >TYJ99727.1 basic blue protein-like [Cucumis melo var. makuwa])

HSP 1 Score: 207.6 bits (527), Expect = 6.0e-50
Identity = 96/125 (76.80%), Postives = 107/125 (85.60%), Query Frame = 0


Query: 1   MGWGRGSA----VLGCLLLVLQYSEMVRAAVYTVGDDQGWTFNVDSWPKQKLFRAGDTLV 60
           MG GRGSA    V+ C+LL+LQYS+M  AAVYTVG  QGWTFNV SWPK K FRAGDTLV
Sbjct: 1   MGEGRGSAMVCVVMVCVLLMLQYSQMAHAAVYTVGGAQGWTFNVASWPKGKRFRAGDTLV 60

Query: 61  FNYSPETHNVVAVNRVGYNRCISPRGSKIFQTGQDQIKLVKGQNFFICSLPGHCQGGMKI 120
           FNYSP  HNVV VNR+GY+RC +PRGSK+FQTG+DQIKLVKGQNFFICSLPGHCQGGMKI
Sbjct: 61  FNYSPSAHNVVGVNRLGYSRCTTPRGSKVFQTGKDQIKLVKGQNFFICSLPGHCQGGMKI 120

Query: 121 AITAL 122
           A+ A+
Sbjct: 121 AVNAI 125

[Close]

BLAST of Lag0019605 vs. NCBI nr
Match: XP_004149964.1 (basic blue protein-like [Cucumis sativus] >KGN57571.1 hypothetical protein Csa_010885 [Cucumis sativus])

HSP 1 Score: 207.2 bits (526), Expect = 7.8e-50
Identity = 95/125 (76.00%), Postives = 107/125 (85.60%), Query Frame = 0


Query: 1   MGWGRGSA----VLGCLLLVLQYSEMVRAAVYTVGDDQGWTFNVDSWPKQKLFRAGDTLV 60
           MG GRGSA    V+ C+LL+LQYS M  AAVYTVG  QGWTFNV SWPK K FRAGDTLV
Sbjct: 1   MGEGRGSAMVCVVMVCMLLMLQYSHMAHAAVYTVGGAQGWTFNVASWPKGKRFRAGDTLV 60

Query: 61  FNYSPETHNVVAVNRVGYNRCISPRGSKIFQTGQDQIKLVKGQNFFICSLPGHCQGGMKI 120
           FNYSP  HNVV VNR+GY+RCI+PRGSK+FQTG+DQIKLVKGQNFFIC++PGHCQGGMKI
Sbjct: 61  FNYSPSAHNVVGVNRLGYSRCITPRGSKVFQTGKDQIKLVKGQNFFICNIPGHCQGGMKI 120

Query: 121 AITAL 122
           A+ A+
Sbjct: 121 AVNAI 125

[Close]

BLAST of Lag0019605 vs. NCBI nr
Match: XP_022985341.1 (basic blue protein-like [Cucurbita maxima])

HSP 1 Score: 205.3 bits (521), Expect = 3.0e-49
Identity = 95/123 (77.24%), Postives = 109/123 (88.62%), Query Frame = 0


Query: 1   MGWGRGSAVLGCL---LLVLQYSEMVRAAVYTVGDDQGWTFNVDSWPKQKLFRAGDTLVF 60
           MG GRGSAV+G +   +L+LQYS++ RAAVYTVG  +GWTFNV SWPK K FRAGDTLVF
Sbjct: 1   MGQGRGSAVVGAVVVCMLLLQYSQVARAAVYTVGGAKGWTFNVASWPKGKRFRAGDTLVF 60

Query: 61  NYSPETHNVVAVNRVGYNRCISPRGSKIFQTGQDQIKLVKGQNFFICSLPGHCQGGMKIA 120
           NYSP  HNVVAVNRVGY+RCI+PRGSK+F+TG+D+IKLVKGQNFFIC+LPGHCQ GMKIA
Sbjct: 61  NYSPAAHNVVAVNRVGYSRCITPRGSKVFKTGKDRIKLVKGQNFFICTLPGHCQAGMKIA 120

[Close]

BLAST of Lag0019605 vs. ExPASy Swiss-Prot
Match: P00303 (Basic blue protein OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 4.8e-34
Identity = 60/96 (62.50%), Postives = 76/96 (79.17%), Query Frame = 0


Query: 26  AVYTVGDDQGWTFNVDSWPKQKLFRAGDTLVFNYSPETHNVVAVNRVGYNRCISPRGSKI 85
           AVY VG   GWTFN +SWPK K FRAGD L+FNY+P  HNVV VN+ G++ C +P G+K+
Sbjct: 1   AVYVVGGSGGWTFNTESWPKGKRFRAGDILLFNYNPXMHNVVVVNQGGFSTCNTPAGAKV 60

Query: 86  FQTGQDQIKLVKGQNFFICSLPGHCQGGMKIAITAL 122
           + +G+DQIKL KGQ++FIC+ PGHCQ GMKIA+ AL
Sbjct: 61  YTSGRDQIKLPKGQSYFICNFPGHCQSGMKIAVNAL 96

[Close]

BLAST of Lag0019605 vs. ExPASy Swiss-Prot
Match: P60496 (Chemocyanin OS=Lilium longiflorum OX=4690 PE=1 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 4.8e-34
Identity = 67/125 (53.60%), Postives = 86/125 (68.80%), Query Frame = 0


Query: 1   MGWGRGSA----VLGCLLLVLQYS-EMVRAAVYTVGDDQGWTFNVDSWPKQKLFRAGDTL 60
           M  G GSA    VLG +L+ L ++ E+  + VYTVGD  GWTF    WP  K FRAGD L
Sbjct: 1   MAQGSGSAERALVLGVVLVFLVFNCEVAESVVYTVGDGGGWTFGTSGWPAGKTFRAGDVL 60

Query: 61  VFNYSPETHNVVAVNRVGYNRCISPRGSKIFQTGQDQIKLVKGQNFFICSLPGHCQGGMK 120
           VF Y+P  HNVV+V   GY  C +  GS++F++G D+I L +G N+FICS+PGHCQGG+K
Sbjct: 61  VFKYNPAVHNVVSVPAGGYKSCTASPGSRVFKSGDDRITLSRGTNYFICSVPGHCQGGLK 120

[Close]

BLAST of Lag0019605 vs. ExPASy Swiss-Prot
Match: Q8LG89 (Basic blue protein OS=Arabidopsis thaliana OX=3702 GN=ARPN PE=2 SV=2)

HSP 1 Score: 141.0 bits (354), Expect = 9.0e-33
Identity = 67/129 (51.94%), Postives = 85/129 (65.89%), Query Frame = 0


Query: 1   MGWGRGSA--------VLGCLLLVLQYSEMVRAAVYTVGDDQGWTFNVDSWPKQKLFRAG 60
           M  GRGSA         L  + ++L  ++ V+AA YTVGD   WTFN   WPK K FRAG
Sbjct: 1   MAKGRGSASWSARAIVTLMAVSVLLLQADYVQAATYTVGDSGIWTFNAVGWPKGKHFRAG 60

Query: 61  DTLVFNYSPETHNVVAVNRVGYNRCISPRGSKIFQTGQDQIKLVKGQNFFICSLPGHCQG 120
           D LVFNY+P  HNVV V+   YN C +P G+K + +G+D+I L KGQNFFIC+ P HC+ 
Sbjct: 61  DVLVFNYNPRMHNVVKVDSGSYNNCKTPTGAKPYTSGKDRITLSKGQNFFICNFPNHCES 120

Query: 121 GMKIAITAL 122
            MKIA+TA+
Sbjct: 121 DMKIAVTAV 129

[Close]

BLAST of Lag0019605 vs. ExPASy Swiss-Prot
Match: A0A072U307 (Blue copper protein 1b OS=Medicago truncatula OX=3880 GN=BCP1B PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 4.4e-19
Identity = 46/111 (41.44%), Postives = 64/111 (57.66%), Query Frame = 0


Query: 15  LVLQYSEMVRAAVYTVGDDQGWT--FNVDSWPKQKLFRAGDTLVFNYSPETHNVVAVNRV 74
           +VL  S  + A  Y VGDD+GWT  F+   W + K+FR GD LVFNY P  HNV  VN  
Sbjct: 14  MVLLSSVAIAATDYIVGDDKGWTVDFDYTQWAQDKVFRVGDNLVFNYDPSRHNVFKVNGT 73

Query: 75  GYNRCISPRGSKIFQTGQDQIKL-VKGQNFFICSLPGHCQG-GMKIAITAL 122
            +  C  P  ++   TG+D I+L  +G+ +++C +  HC    MK+ IT L
Sbjct: 74  LFQSCTFPPKNEALSTGKDIIQLKTEGRKWYVCGVADHCSARQMKLVITVL 124

[Close]

BLAST of Lag0019605 vs. ExPASy Swiss-Prot
Match: A0A0M4FTF3 (Blue copper protein OS=Petunia hybrida OX=4102 GN=BCP PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.3e-18
Identity = 45/96 (46.88%), Postives = 60/96 (62.50%), Query Frame = 0


Query: 28  YTVGDDQGWTFNVD--SWPKQKLFRAGDTLVFNYSPETHNVVAVNRVGYNRCISPRGSKI 87
           Y VGDD+GWT +VD  +W K K F+ GDTLVF Y+   HNV  VN+ G+  CI+P  S+ 
Sbjct: 118 YWVGDDKGWTIDVDYQAWAKGKTFKVGDTLVFKYTKGHHNVFKVNQTGFQNCIAPPPSEG 177

Query: 88  FQTGQDQIKL-VKGQNFFICSLPGHC-QGGMKIAIT 120
             +G D I L   G+ ++IC  P HC +   K+AIT
Sbjct: 178 LTSGHDVITLAAPGKKWYICGFPTHCSEHKQKLAIT 213

The following BLAST results are available for this feature:

BLAST of Lag0019605 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878143.1	1.9e-51	80.80	basic blue protein-like [Benincasa hispida]	[more]
XP_038903112.1	1.6e-50	78.40	basic blue protein-like [Benincasa hispida]	[more]
XP_008465594.1	6.0e-50	76.80	PREDICTED: basic blue protein-like [Cucumis melo] >KAA0039078.1 basic blue prote...	[more]
XP_004149964.1	7.8e-50	76.00	basic blue protein-like [Cucumis sativus] >KGN57571.1 hypothetical protein Csa_0...	[more]
XP_022985341.1	3.0e-49	77.24	basic blue protein-like [Cucurbita maxima]	[more]

BLAST of Lag0019605 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P00303	4.8e-34	62.50	Basic blue protein OS=Cucumis sativus OX=3659 PE=1 SV=1	[more]
P60496	4.8e-34	53.60	Chemocyanin OS=Lilium longiflorum OX=4690 PE=1 SV=1	[more]
Q8LG89	9.0e-33	51.94	Basic blue protein OS=Arabidopsis thaliana OX=3702 GN=ARPN PE=2 SV=2	[more]
A0A072U307	4.4e-19	41.44	Blue copper protein 1b OS=Medicago truncatula OX=3880 GN=BCP1B PE=2 SV=1	[more]
A0A0M4FTF3	1.3e-18	46.88	Blue copper protein OS=Petunia hybrida OX=4102 GN=BCP PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 26..120 e-value: 2.1E-31 score: 110.0
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 26..120
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 36..114 e-value: 5.0E-24 score: 84.1
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 26..121 score: 34.802578
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 9..119
None	No IPR available	PANTHER	PTHR33021:SF379	BLUE PROTEIN, PUTATIVE-RELATED	coord: 9..119
IPR041844	Plantacyanin	CDD	cd11013	Plantacyanin	coord: 26..120 e-value: 9.57547E-51 score: 154.049

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0019605.1	Lag0019605.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0046658	anchored component of plasma membrane
molecular_function	GO:0009055	electron transfer activity