Lag0019541 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0019541
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Enolase-phosphatase E1
Location	chr5: 43163986 .. 43168717 (+)
RNA-Seq Expression	Lag0019541
Synteny	Lag0019541

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCAATGGCATCGGCTCTGGTCGTCAATGGAGTCAAGTTGGCTTCAACTTCTCAAGCTTATTTGGAAACCAAGGCCGCCAACGACACCAAGGCTCTCTTATCGGAGCTCTACCGCCAATTCTACGGCGTCCGATCGGTCTCCGGCGCCGGCGGCAGCATTGCCATCAAGGTGCACCACCACTCCATTCCCAAGAGACAACAGCCAATCGCTATGTCACCTTCTGGTGGGCATTTCTCCATCACTCCATTCTTTTCTTCCTTGTTTAATTTATTACAATTTCTGGGCATTTTGTTTCTTGCATTGCTTTCAATCGAGCTAATTGGATTTCTTTGTCTCTTGGGTAAGTGATTTTCGAGGTTTCCCCCAATTTTCCTGTAGTCTTTTTGGTGTAGAATTTTTGTTATTGAGGTATATCCTTCTGTCTCTACTCTGCTTTCAGTTGTGTTTATTTCTCGTTTCTTTGAATTTGCTATGAAATCTATAATGGTGAAGATTATTTTTTAGCTTTCCTTATGTTTGTTTGATGCTATAGTCCATAGTGGTTTTAAAATTACTTAAACATACTTAAGGAAAACACTTCGTTAGTGTTTCCCCTAAAAAATGCTTTAAAAGAAAGTGTCACTTAAAAACACTACCTCAAAATCATCTATCAAACCCGCCCTCAAGATGTGTTTAGGTTTAGGAGTGCTTTTAAAATAAGTACTATGCTTAATAATAAATCACTCTAATGTTTAGTTTTGTACTTTTAAAAGTACTTTTGAAGTGCTTCTCAATCAATCAAACACCTAGGCTTAAAAGTACTTGCTTGTTAACCTAACAATATAAGGTGGTTTTTTACATTTTTCAACATGATGATTTTCGATGGAGAGTGCTTCAGGCCACTTCCAAATATAGCCTTATGCTTCTCAATTTCCTAGCTTTTGGGTTAGTAGTCTGTCAAACAAGGTTGGCTGTTTGTAACTGGCGTATGAATATGGCCAGGCATCGTTATAATTTAAACTAGTGCTTGTTGGGAGGATATCCAGTTTCCCCTTCTTTTTGCAGAAGAAGAAATTGTTCTTCTTTGAACCAAAAATGCTTTTCAAGGGTTCAAGCATCAAGAACTGTAAAGAATGTATTTACATGGTGTAAGATCACCTGCTTTTCCGTATTTTCTTTATCCTTTTTGTTTGGCAAGAGGGTATTATATGCAATTCAACTCCTTGCATAAGAGTATCGAGAGCTTGTTCCTTCTCTTTTTTCCTTTCCCCTTAAAAATCAATCGCTTGGAATTCATGGATTTGGATGAATATTCTTTTGCTTTTGTTGGATTGTCCATAGGTGTTAAGAAGGAGAGAATGATACTGGAGGACATCTATGTGTTATCTCATAGTGGGTCTATCTTATATTCACCACCTCCAAAGCCATTTTCACATAAGCTTCCAAACCGTTGTGGACCTCTTTTTATGAAGGTACAGTTCTCGGTACTCTGTAAATACTCATTTGGACGAGGACTTAAAAAACGTCATAAGCTAAACCCATGCTGACAAATAAGCAACTTGTAATGAATAGAAAAGATATAGTCATGCTATAAATGACCCGGTATCTCTCTTCCTTCTCAGTACATTTTATGATTTCGGTGTTTCTTATTAAACAACAATAACTATAGTTGTAGCATTATTTTAAGTAAGCAGCAGGTTCGTTTTGTGATTTTGCACCTTAAATCTCAAGATTATTCAGCACTCTATGTACTTTTTCTTACCATCCCTTTTTATTTGATAAAATACATCTAGCAAGAAGTTTCTTTGAACTTTGTAGATGATGAAATTTCTTATTTTGTACAATTTCAAGAAATTGATTTTGAGTCTGTCTTGATCTTTCATTTAGTTTCAGTTTAATCAATAACTTAATTTTACAGGCTGATTGTTATCACTGTCTCTCTTTGATGCTGGTATTAAGCTTCGTCAACTAGGCATTGATTAGTCTTCTCCAACTCAGGGTCCGATTGGGAGCATCAAAGGAATTTCAAGAGATGGCTTTGGCATCAACATTTCTGTGAAGGCTGGGGCTCAAGGTTTAGATAATAAAAACGAGCCATTGCCAGTGAGTTGAATTGCATTCTACTTAATTGGGAAGCTTAAAATTTCAGCCTTTAATTTTTCATTCTTTTCTTTTCCCTTTGAGATATGCATTTTTGTCTCTTAATTATAAGGTTTTTGTTTATGGTTTGAGTTTGATCAGTCTTCCTTTCAATTGTGGTTCATTTCTCATTTATATTTTGGATGCAGCGTTGTATAGTTCTTGATATCGAAGGGACTACTACTCCAATATCCTTTGTTACTGATGTACTCTTTCCATATGCTCGTGATAATGTTGGAAAGCATTTGAGTTTAACGTACGAAACTGCAGAAACTCAAGATGACATTAAGTTGTTACGTTCACAAGTAAGATATTTTGGTTAAACAGCGTTTTGATTATGTTGCAGTCTGTAGAATGTCTATGTTCCATTTGTGGTATTTACTTGGGGATTGTTGGCTTAGTTGAACTGAAAAATAGCTTGTGCATTTGATAGGAATGTAAGTAAGGTGTAATTAGGAATATTGGTAAGGATATATTAGCAATTAGTTAGGGAGTTGTTAGAGTATGGAGTTGTAAATAGACGGAGTGGGAAAGGGGAAGATACAGAATGAAGTCAGTCAGTTAGATCTTGAGTGATCTCAAGAGAGGTAGGGCCAAGTACCTCGAATACTTGGATTTATCGTGTAGTCCTTTATCTTTTATATTGTAATACATTGTGGTTCTGTTCTGGTTTCTATCGGCGTCTATGAGTTTATCTGTGAAATACAGAAACTTCCTAGCAGCAAATCCAATTTGGTACTTGACAGTTATAGGCTATTTGGGAGTTGTTTTCTGAACCTTCTGCTTTTCAGTATAAAGATTCAGAGAAAATATGTTTGGATAACTAAATTCTAAATGTTCTAAATTTGCCAATGAATTACAACGTCCTTGATGGCAAACATCCTAGATAGATTTGCATCTTGTAATAAAAAACCAATTGATGCTTCTAGTTCGACAACTGGACATTCAGTAAATACAGTTACGTTTTTGGCATAAGAAGGGCCTTCTTTGTAATTATTTATAAAATGCGTTCAGGTTGAAGATGACTTAGAAAAGGGTGTTGCTGGAGCGGTGCCTGTTCCTCCTGATAATGCTGCTAAAGAGGAAGTCATTGCAGCATTGGTTGCTAATGTTGAGGCAATGATAAAAGCTGACCGCAAGATAACTGCCTTGAAACAATTGCAAGTATGCAAGTAAAATTATTCTCATCTTACTGCAATTCATGTAGCAGATTTTGTGAGTGAGCTGACTTCTAGCTATGCAGGGTCACATATGGAGAACAGGGTTTTCAGGCAATGAATTGGAGGGATTAGTTTTTGACGATGTACCAGAGGCACTCAAGAGATGGCATGCTTTGGGCATTAAGGTCATTCCAACCCAACCCCCTTCTTGATTTTTTCTAACTTAAACCAGTCACTTCATGCAAAAAGAAAATGTCACACCTGCACTGACACATTGCTATTTGAGACTAACCCGGGAAAGGTCCTCCCAATGCTAACTCCCACACTGGATATAAACTCAATGATATCAGGACATTTAGAACCCAACTCAGCTTTAATTGAAGAATGATCCAACCTTTTTTAACATACTACGACCCCAAGGATGTATTGTGTGACATTTTGATTCCTCGTTACTAATAGAATCAAAAAATTATCTTTCTTGATCTCTCCCAATTCAACAATCCAAGATTTGATTTAGTGTCCGTTTGAGTCCTCTAAATCACATTGATACGTAGAGAAGACTAAACTCAAGCTTACTTAAGTCCCTTTGCATATGTGTTCATTTTCCTCACAGTGGAATCTATTTAGTGAAGACTAAACTCCTAGTCCTAGTTATTAGATGAAATTGATAGTTTTATGCTTGGGTTAGTAGATGACAGGCCTCTCTAAAATTAGATTTTCTTTTGATTGATTTTCCTTCATTCTCATTTGGAAGCCTGGTGATATTTGAACAAAAGTTCTCCAACAGATGTTGCGAACCAGTAATAACTTTCTGGCATGAACAGGTCTACATATATTCAAGCGGCAGCAGGTTGGCACAAAGGCTTATATTTGGAAAGACTAATTATGGTGATCTAAGAAAATACTTATCAGGATTCTTTGACACTGCTGTGGGGTAACCAGCTGCTGTTTTTTTCCCCCTTTTTGTTTTCCATGTTTTAATCAGACTTAACATCACAACCATGATGGTGAAACTGCTCTTGTATCTCAGGAACAAAAGAGAAATAAGCAGTTATGTTGAGATTTCAGAGTCTGTTGGAGTTGATACACCATCAGAAATATTGTTCATCACAGATGTGTATCAGGAAGCTGTAGCAGCAAAAGCAGCAGGTAATGAGTCTATCCTTTTAGCTATTGTTTGTTGTTAGTTTATGGAAGAAAAGTGAGTAAAATCCATTCGTGCTATAACCATTGGAATTGCATTGGGCTGAAAAGTTATAGTGAAAACTATCATATGAAGTTTCAAAAGAATTGCCAAGTTAATCGCAATGCAGATTTACTAATTTCATACTTCTTGGAATGCAATCGACGACGTTTCAGGGCTGCAGGTGGCAATCTCAATCCGACCAGGAAATGGCCCACTACCTGATGATCATGGGTTCCAGACAATCAATTCCTTCTCTGAAATCTGA

mRNA sequence

ATGCCAATGGCATCGGCTCTGGTCGTCAATGGAGTCAAGTTGGCTTCAACTTCTCAAGCTTATTTGGAAACCAAGGCCGCCAACGACACCAAGGCTCTCTTATCGGAGCTCTACCGCCAATTCTACGGCGTCCGATCGGTCTCCGGCGCCGGCGGCAGCATTGCCATCAAGGTGCACCACCACTCCATTCCCAAGAGACAACAGCCAATCGCTATGTCACCTTCTGGTGGGCATTTCTCCATCACTCCATTCTTTTCTTCCTTGTTTAATTTATTACAATTTCTGGGCATTTTGTTTCTTGCATTGCTTTCAATCGAGCTAATTGGATTTCTTTGTCTCTTGGGTGTTAAGAAGGAGAGAATGATACTGGAGGACATCTATGTGTTATCTCATAGTGGGTCTATCTTATATTCACCACCTCCAAAGCCATTTTCACATAAGCTTCCAAACCGTTGTGGACCTCTTTTTATGAAGGGTCCGATTGGGAGCATCAAAGGAATTTCAAGAGATGGCTTTGGCATCAACATTTCTGTGAAGGCTGGGGCTCAAGGTTTAGATAATAAAAACGAGCCATTGCCACGTTGTATAGTTCTTGATATCGAAGGGACTACTACTCCAATATCCTTTGTTACTGATGTACTCTTTCCATATGCTCGTGATAATGTTGGAAAGCATTTGAGTTTAACGTACGAAACTGCAGAAACTCAAGATGACATTAAGTTGTTACGTTCACAAGTTGAAGATGACTTAGAAAAGGGTGTTGCTGGAGCGGTGCCTGTTCCTCCTGATAATGCTGCTAAAGAGGAAGTCATTGCAGCATTGGTTGCTAATGTTGAGGCAATGATAAAAGCTGACCGCAAGATAACTGCCTTGAAACAATTGCAAGGTCACATATGGAGAACAGGGTTTTCAGGCAATGAATTGGAGGGATTAGTTTTTGACGATGTACCAGAGGCACTCAAGAGATGGCATGCTTTGGGCATTAAGGTCTACATATATTCAAGCGGCAGCAGGTTGGCACAAAGGCTTATATTTGGAAAGACTAATTATGGTGATCTAAGAAAATACTTATCAGGATTCTTTGACACTGCTGTGGGGAACAAAAGAGAAATAAGCAGTTATGTTGAGATTTCAGAGTCTGTTGGAGTTGATACACCATCAGAAATATTGTTCATCACAGATGTGTATCAGGAAGCTGTAGCAGCAAAAGCAGCAGGGCTGCAGGTGGCAATCTCAATCCGACCAGGAAATGGCCCACTACCTGATGATCATGGGTTCCAGACAATCAATTCCTTCTCTGAAATCTGA

Coding sequence (CDS)

ATGCCAATGGCATCGGCTCTGGTCGTCAATGGAGTCAAGTTGGCTTCAACTTCTCAAGCTTATTTGGAAACCAAGGCCGCCAACGACACCAAGGCTCTCTTATCGGAGCTCTACCGCCAATTCTACGGCGTCCGATCGGTCTCCGGCGCCGGCGGCAGCATTGCCATCAAGGTGCACCACCACTCCATTCCCAAGAGACAACAGCCAATCGCTATGTCACCTTCTGGTGGGCATTTCTCCATCACTCCATTCTTTTCTTCCTTGTTTAATTTATTACAATTTCTGGGCATTTTGTTTCTTGCATTGCTTTCAATCGAGCTAATTGGATTTCTTTGTCTCTTGGGTGTTAAGAAGGAGAGAATGATACTGGAGGACATCTATGTGTTATCTCATAGTGGGTCTATCTTATATTCACCACCTCCAAAGCCATTTTCACATAAGCTTCCAAACCGTTGTGGACCTCTTTTTATGAAGGGTCCGATTGGGAGCATCAAAGGAATTTCAAGAGATGGCTTTGGCATCAACATTTCTGTGAAGGCTGGGGCTCAAGGTTTAGATAATAAAAACGAGCCATTGCCACGTTGTATAGTTCTTGATATCGAAGGGACTACTACTCCAATATCCTTTGTTACTGATGTACTCTTTCCATATGCTCGTGATAATGTTGGAAAGCATTTGAGTTTAACGTACGAAACTGCAGAAACTCAAGATGACATTAAGTTGTTACGTTCACAAGTTGAAGATGACTTAGAAAAGGGTGTTGCTGGAGCGGTGCCTGTTCCTCCTGATAATGCTGCTAAAGAGGAAGTCATTGCAGCATTGGTTGCTAATGTTGAGGCAATGATAAAAGCTGACCGCAAGATAACTGCCTTGAAACAATTGCAAGGTCACATATGGAGAACAGGGTTTTCAGGCAATGAATTGGAGGGATTAGTTTTTGACGATGTACCAGAGGCACTCAAGAGATGGCATGCTTTGGGCATTAAGGTCTACATATATTCAAGCGGCAGCAGGTTGGCACAAAGGCTTATATTTGGAAAGACTAATTATGGTGATCTAAGAAAATACTTATCAGGATTCTTTGACACTGCTGTGGGGAACAAAAGAGAAATAAGCAGTTATGTTGAGATTTCAGAGTCTGTTGGAGTTGATACACCATCAGAAATATTGTTCATCACAGATGTGTATCAGGAAGCTGTAGCAGCAAAAGCAGCAGGGCTGCAGGTGGCAATCTCAATCCGACCAGGAAATGGCCCACTACCTGATGATCATGGGTTCCAGACAATCAATTCCTTCTCTGAAATCTGA

Protein sequence

MPMASALVVNGVKLASTSQAYLETKAANDTKALLSELYRQFYGVRSVSGAGGSIAIKVHHHSIPKRQQPIAMSPSGGHFSITPFFSSLFNLLQFLGILFLALLSIELIGFLCLLGVKKERMILEDIYVLSHSGSILYSPPPKPFSHKLPNRCGPLFMKGPIGSIKGISRDGFGINISVKAGAQGLDNKNEPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLSLTYETAETQDDIKLLRSQVEDDLEKGVAGAVPVPPDNAAKEEVIAALVANVEAMIKADRKITALKQLQGHIWRTGFSGNELEGLVFDDVPEALKRWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREISSYVEISESVGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTINSFSEI

Homology

[Close]

BLAST of Lag0019541 vs. NCBI nr
Match: TYK11241.1 (putative bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 671.0 bits (1730), Expect = 6.9e-189
Identity = 365/466 (78.33%), Postives = 387/466 (83.05%), Query Frame = 0


Query: 1   MPMASALVVNGVKLASTSQAYLETKAANDTKALLSELYRQFYGVRSVSGAGGSIAIKVHH 60
           M MASAL ++GVKLA+TSQAY+ETKAANDTKALLSEL RQFY +R VSG G SIAIKVH 
Sbjct: 1   MAMASALPLSGVKLATTSQAYMETKAANDTKALLSELSRQFYTLRCVSGTGSSIAIKVHD 60

Query: 61  HSIPKRQQPIAMSPSGGHFSITPFFSSL----FNLLQFLGILFLALLSIELIGFLCLLGV 120
           HSIP+RQQ IAMS SGGH SI  F  S+    FN L FL IL L+LL IELIG +CLLGV
Sbjct: 61  HSIPRRQQLIAMSLSGGHLSILIFPLSVLVFPFNFLHFLAILLLSLLPIELIGSVCLLGV 120

Query: 121 KKERMILEDIYVLSHSGSILYSPPPKPFSHKLPN--RCGPLFMK---------------- 180
           KK RM+ ED Y+LS  GSIL+SPPPKPFSHKLPN     PLFMK                
Sbjct: 121 KKRRMVREDTYMLSSGGSILHSPPPKPFSHKLPNCFDSRPLFMKMRNAGAVIHSHSKESY 180

Query: 181 ---------GPIGSIKGISRDGFGINISVKAGAQGLDNKNEPLPRCIVLDIEGTTTPISF 240
                    G IGSIKGIS      NISVKAGA+GLDNKN+  PRCIVLDIEGTTTPISF
Sbjct: 181 LVTQRNHHQGQIGSIKGISS-----NISVKAGARGLDNKNQWFPRCIVLDIEGTTTPISF 240

Query: 241 VTDVLFPYARDNVGKHLSLTYETAETQDDIKLLRSQVEDDLEKGVAGAVPVPPDNAAKEE 300
           VTDVLFPYARDNV KHL LTYET ETQDDIKLLRSQVE+DLEKGVAGAVP+PPDNA KEE
Sbjct: 241 VTDVLFPYARDNVEKHLILTYETGETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEE 300

Query: 301 VIAALVANVEAMIKADRKITALKQLQGHIWRTGFSGNELEGLVFDDVPEALKRWHALGIK 360
           VIAA+VANVE MIKADRKITALKQLQGHIWRTGFSGNELEG+VFDDVPEAL+RWHA GIK
Sbjct: 301 VIAAVVANVEGMIKADRKITALKQLQGHIWRTGFSGNELEGVVFDDVPEALERWHASGIK 360

Query: 361 VYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREISSYVEISESVGVDTPSEI 420
           +YIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKRE SSYVEISESVGVD+PSEI
Sbjct: 361 MYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKRETSSYVEISESVGVDSPSEI 420

Query: 421 LFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTINSFSEI 436
           LFITDV QEA AAKAAGLQVAISIRPGNGPLPD+HGFQTI SFSEI
Sbjct: 421 LFITDVCQEAKAAKAAGLQVAISIRPGNGPLPDNHGFQTITSFSEI 461

[Close]

BLAST of Lag0019541 vs. NCBI nr
Match: KAA0058822.1 (putative bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 667.9 bits (1722), Expect = 5.8e-188
Identity = 364/466 (78.11%), Postives = 386/466 (82.83%), Query Frame = 0


Query: 1   MPMASALVVNGVKLASTSQAYLETKAANDTKALLSELYRQFYGVRSVSGAGGSIAIKVHH 60
           M MASAL ++GVKLA+TSQAY+ETKAANDTKALLSEL RQFY +R VSG G SIAIKVH 
Sbjct: 1   MAMASALPLSGVKLATTSQAYMETKAANDTKALLSELSRQFYTLRCVSGTGSSIAIKVHD 60

Query: 61  HSIPKRQQPIAMSPSGGHFSITPFFSSL----FNLLQFLGILFLALLSIELIGFLCLLGV 120
           HSIP+RQQ IAMS SGGH SI  F  S+    FN L FL IL L+LL IELIG +CLLGV
Sbjct: 61  HSIPRRQQLIAMSLSGGHLSILIFPLSVLVFPFNFLHFLAILLLSLLPIELIGSVCLLGV 120

Query: 121 KKERMILEDIYVLSHSGSILYSPPPKPFSHKLPN--RCGPLFMK---------------- 180
           KK RM+ ED Y+LS  GSIL+SPPPKPFSHKLPN     PLFMK                
Sbjct: 121 KKRRMVREDTYMLSSGGSILHSPPPKPFSHKLPNCFDSRPLFMKMRNAGAVIHSHSKESY 180

Query: 181 ---------GPIGSIKGISRDGFGINISVKAGAQGLDNKNEPLPRCIVLDIEGTTTPISF 240
                    G IGSIKGIS      NISVKAGA+GLDNKN+  PRCIVLDIEGTTTPISF
Sbjct: 181 LVTQRNHHQGQIGSIKGISS-----NISVKAGARGLDNKNQWFPRCIVLDIEGTTTPISF 240

Query: 241 VTDVLFPYARDNVGKHLSLTYETAETQDDIKLLRSQVEDDLEKGVAGAVPVPPDNAAKEE 300
           VTDVLF YARDNV KHL LTYET ETQDDIKLLRSQVE+DLEKGVAGAVP+PPDNA KEE
Sbjct: 241 VTDVLFSYARDNVEKHLILTYETGETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEE 300

Query: 301 VIAALVANVEAMIKADRKITALKQLQGHIWRTGFSGNELEGLVFDDVPEALKRWHALGIK 360
           VIAA+VANVE MIKADRKITALKQLQGHIWRTGFSGNELEG+VFDDVPEAL+RWHA GIK
Sbjct: 301 VIAAVVANVEGMIKADRKITALKQLQGHIWRTGFSGNELEGVVFDDVPEALERWHASGIK 360

Query: 361 VYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREISSYVEISESVGVDTPSEI 420
           +YIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKRE SSYVEISESVGVD+PSEI
Sbjct: 361 MYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKRETSSYVEISESVGVDSPSEI 420

Query: 421 LFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTINSFSEI 436
           LFITDV QEA AAKAAGLQVAISIRPGNGPLPD+HGFQTI SFSEI
Sbjct: 421 LFITDVCQEAKAAKAAGLQVAISIRPGNGPLPDNHGFQTITSFSEI 461

[Close]

BLAST of Lag0019541 vs. NCBI nr
Match: XP_038899594.1 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 [Benincasa hispida])

HSP 1 Score: 654.1 bits (1686), Expect = 8.7e-184
Identity = 350/437 (80.09%), Postives = 368/437 (84.21%), Query Frame = 0


Query: 1   MPMASALVVNGVKLASTSQAYLETKAANDTKALLSELYRQFYGVRSVSGAGGSIAIKVHH 60
           M MASAL VNG+KLA+TSQAYLE+KAANDTKALLSEL RQF G+R VSG GGSIAI VH 
Sbjct: 1   MAMASALAVNGLKLATTSQAYLESKAANDTKALLSELSRQFDGLRWVSGTGGSIAITVHD 60

Query: 61  HSIPKRQQPIAMSPSGGHFSITPFFSSLFNLLQFLGILFLALLSIELIGFLCLLGVKKER 120
           +SIPKR++ IAMSPS                                       GVKKER
Sbjct: 61  YSIPKRRRLIAMSPS---------------------------------------GVKKER 120

Query: 121 MILEDIYVLSHSGSILYSPPPKPFSHKLPN--RCGPLFMKGPIGSIKGISRDGFGINISV 180
           M+ ED   LS++GSILYSPPPKPFS++LPN   CGPLFMKG IGSIKGIS      NISV
Sbjct: 121 MLREDTCKLSYAGSILYSPPPKPFSYELPNCFDCGPLFMKGRIGSIKGISS-----NISV 180

Query: 181 KAGAQGLDNKNEPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLSLTYETAETQDD 240
           KAG QGLDNKNE LPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHL LTYETAET+DD
Sbjct: 181 KAGVQGLDNKNESLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLILTYETAETRDD 240

Query: 241 IKLLRSQVEDDLEKGVAGAVPVPPDNAAKEEVIAALVANVEAMIKADRKITALKQLQGHI 300
           IKLLRSQVE+DLEKGVAGAVP+PPDNA KEEVIAALVANVEAMIKADRKITALKQLQGHI
Sbjct: 241 IKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMIKADRKITALKQLQGHI 300

Query: 301 WRTGFSGNELEGLVFDDVPEALKRWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLS 360
           WRTGFSGNELEG+VFDDVPEAL+RWHA GIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLS
Sbjct: 301 WRTGFSGNELEGVVFDDVPEALERWHASGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLS 360

Query: 361 GFFDTAVGNKREISSYVEISESVGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNG 420
           GFFDTAVGNKREISSYVEISESVGVDTPSEILFITDV QEAVAAKAAGLQVAISIRPGNG
Sbjct: 361 GFFDTAVGNKREISSYVEISESVGVDTPSEILFITDVCQEAVAAKAAGLQVAISIRPGNG 393

Query: 421 PLPDDHGFQTINSFSEI 436
           PLPD+HGFQTI SFSEI
Sbjct: 421 PLPDNHGFQTITSFSEI 393

[Close]

BLAST of Lag0019541 vs. NCBI nr
Match: XP_022923008.1 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X3 [Cucurbita moschata])

HSP 1 Score: 638.3 bits (1645), Expect = 4.9e-179
Identity = 337/437 (77.12%), Postives = 359/437 (82.15%), Query Frame = 0


Query: 1   MPMASALVVNGVKLASTSQAYLETKAANDTKALLSELYRQFYGVRSVSGAGGSIAIKVHH 60
           M MAS L VNGVKLA+ S AY++TKAAN TKAL++EL RQFYG+R VSG  G I+IKVH 
Sbjct: 1   MAMASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHD 60

Query: 61  HSIPKRQQPIAMSPSGGHFSITPFFSSLFNLLQFLGILFLALLSIELIGFLCLLGVKKER 120
           HSIPK Q  +A+S S                                       GVKKER
Sbjct: 61  HSIPKGQHTVALSHS---------------------------------------GVKKER 120

Query: 121 MILEDIYVLSHSGSILYSPPPKPFSHKLPNRC--GPLFMKGPIGSIKGISRDGFGINISV 180
           M+ ED  +LS+ GSILYSPPPKPFSHKLP     GPLFMK P GSIKG+S       ISV
Sbjct: 121 MVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFMKDPFGSIKGMSS-----KISV 180

Query: 181 KAGAQGLDNKNEPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLSLTYETAETQDD 240
           KAGAQGLDN+N+PLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHL LTYETAETQDD
Sbjct: 181 KAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLILTYETAETQDD 240

Query: 241 IKLLRSQVEDDLEKGVAGAVPVPPDNAAKEEVIAALVANVEAMIKADRKITALKQLQGHI 300
           IKLLRSQVE+DLEKGVAGAVP+PPDNA KEEVIAALVANVEAMIKADRKITALKQLQGHI
Sbjct: 241 IKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMIKADRKITALKQLQGHI 300

Query: 301 WRTGFSGNELEGLVFDDVPEALKRWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLS 360
           WRTGF+GNELEG+VFDDVPEAL+RWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLS
Sbjct: 301 WRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLS 360

Query: 361 GFFDTAVGNKREISSYVEISESVGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNG 420
           GFFDTAVGNKREI SYVEISES+GVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNG
Sbjct: 361 GFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNG 393

Query: 421 PLPDDHGFQTINSFSEI 436
           PLPDDHGFQT  SFS+I
Sbjct: 421 PLPDDHGFQTATSFSDI 393

[Close]

BLAST of Lag0019541 vs. NCBI nr
Match: XP_023551888.1 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 [Cucurbita pepo subsp. pepo] >XP_023551890.1 probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 635.6 bits (1638), Expect = 3.2e-178
Identity = 337/437 (77.12%), Postives = 358/437 (81.92%), Query Frame = 0


Query: 1   MPMASALVVNGVKLASTSQAYLETKAANDTKALLSELYRQFYGVRSVSGAGGSIAIKVHH 60
           M MAS L VNGVKLA+ S AY++TKAAN+TKAL++EL RQFYG+R VS   GSIAIKVH 
Sbjct: 1   MAMASPLAVNGVKLATASLAYVDTKAANNTKALMAELSRQFYGLRLVSRPAGSIAIKVHD 60

Query: 61  HSIPKRQQPIAMSPSGGHFSITPFFSSLFNLLQFLGILFLALLSIELIGFLCLLGVKKER 120
           HSIPK Q  +A+S S                                       GVKKER
Sbjct: 61  HSIPKGQHTVALSHS---------------------------------------GVKKER 120

Query: 121 MILEDIYVLSHSGSILYSPPPKPFSHKLPNRC--GPLFMKGPIGSIKGISRDGFGINISV 180
           M+ ED  +LS+  SILYSPPPKPFSHKLPN    GPLFMK P G IKGIS       ISV
Sbjct: 121 MVAEDTSMLSYGVSILYSPPPKPFSHKLPNCLDGGPLFMKDPFGRIKGISS-----KISV 180

Query: 181 KAGAQGLDNKNEPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLSLTYETAETQDD 240
           KAG QGLDN+N+PLPRCIVLDIEGTTTPISFVTDVLFPYARD+VGKHL LTYETAETQDD
Sbjct: 181 KAGTQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPYARDSVGKHLLLTYETAETQDD 240

Query: 241 IKLLRSQVEDDLEKGVAGAVPVPPDNAAKEEVIAALVANVEAMIKADRKITALKQLQGHI 300
           IKLLRSQVE+DLEKGVAGAVP+PPDNA KEEVIAALVANVEAMIKADRKITALKQLQGHI
Sbjct: 241 IKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMIKADRKITALKQLQGHI 300

Query: 301 WRTGFSGNELEGLVFDDVPEALKRWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLS 360
           WRTGFSGNELEG+VFDDVPEAL+RWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLS
Sbjct: 301 WRTGFSGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLS 360

Query: 361 GFFDTAVGNKREISSYVEISESVGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNG 420
           GFFDTAVGNKREI SYVEISES+GVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNG
Sbjct: 361 GFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNG 393

Query: 421 PLPDDHGFQTINSFSEI 436
           PLPDDHGFQT  SFS+I
Sbjct: 421 PLPDDHGFQTATSFSDI 393

[Close]

BLAST of Lag0019541 vs. ExPASy Swiss-Prot
Match: E0CSI1 (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 OS=Vitis vinifera OX=29760 GN=VIT_19s0014g02480 PE=3 SV=2)

HSP 1 Score: 502.7 bits (1293), Expect = 4.3e-141
Identity = 293/555 (52.79%), Postives = 335/555 (60.36%), Query Frame = 0


Query: 5   SALVVNGVKLASTSQAYLETKAANDTKALLSELYRQFYGVRSVSGAGGSIAIKVHHHSIP 64
           +A  +NG+K+A+TSQAYLE+   NDT+ LLS+L R FYG+  VSG GGSI IKVH  SIP
Sbjct: 2   AAAALNGLKMAATSQAYLESMPVNDTRKLLSDLCRHFYGLGWVSGTGGSITIKVHDESIP 61

Query: 65  KRQQPIAMSPSGGHFSITPFFSSLFNLLQFLGILFLALLSIELIGFLCLLGVKKERMILE 124
           K QQ I MSPS                                       GV+KERM+ E
Sbjct: 62  KPQQLIVMSPS---------------------------------------GVQKERMVPE 121

Query: 125 DIYVLSHSGSILYSPPPKPFSHKLP--NRCGPLFMKG----------------------- 184
           D+YVLS SG  L SP PKP+ +K P  + C PLFMK                        
Sbjct: 122 DMYVLSPSGCFLSSPSPKPYPNKPPKCSDCAPLFMKAYLMRNAGAVIHSHGMESCIVTMI 181

Query: 185 -PIGS---------IKGISRDGF------------------------------------- 244
            P+           IKGI   G+                                     
Sbjct: 182 DPLSKEFRITHMEMIKGIQGHGYYDELVVPIIENTAHERELTDALAEAIEAYPKTTAVLV 241

Query: 245 ---GI-------------------------------------------------NISVKA 304
              GI                                                 N+SVKA
Sbjct: 242 RNHGIYIWGDSWIHAKTQAECYHYLFDAAIKLYQLGLDWSTPDHGPIKKYGGKTNMSVKA 301

Query: 305 GAQGLDNKNEPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLSLTYETAETQDDIK 364
           G    +++ EP  RCIVLDIEGTTTPISFVTDVLFP+AR+NV +HL+ TYET ETQDDIK
Sbjct: 302 GTVNSNHETEPSRRCIVLDIEGTTTPISFVTDVLFPFARNNVSRHLAATYETDETQDDIK 361

Query: 365 LLRSQVEDDLEKGVAGAVPVPPDNAAKEEVIAALVANVEAMIKADRKITALKQLQGHIWR 424
           LLR+QV+ DLE+G+ GAVP+PPD+A KEEVIAALVANVEAMIKADRKITALKQLQGHIWR
Sbjct: 362 LLRTQVQSDLEQGIVGAVPIPPDDAGKEEVIAALVANVEAMIKADRKITALKQLQGHIWR 421

Query: 425 TGFSGNELEGLVFDDVPEALKRWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGF 436
           TGF  NELEG+VF+DVPEAL++WHA GIKVYIYSSGSRLAQRL+FG TNYGDLRKYL GF
Sbjct: 422 TGFQNNELEGVVFEDVPEALQKWHASGIKVYIYSSGSRLAQRLLFGYTNYGDLRKYLCGF 481

[Close]

BLAST of Lag0019541 vs. ExPASy Swiss-Prot
Match: B9N1F9 (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Populus trichocarpa OX=3694 GN=POPTR_0001s40980g PE=3 SV=2)

HSP 1 Score: 491.1 bits (1263), Expect = 1.3e-137
Identity = 286/551 (51.91%), Postives = 333/551 (60.44%), Query Frame = 0


Query: 6   ALVVNGVKLAS---TSQAYLETKAANDTKALLSELYRQFYGVRSVSGAGGSIAIKVHHHS 65
           A+ VNG  +A+    SQAYLE+KA  DT+ L+++L +QFY +  VSG GGSI IK H  S
Sbjct: 7   AVAVNGGGMAAAKVASQAYLESKAVKDTRVLIADLCKQFYTLGWVSGTGGSITIKAHDDS 66

Query: 66  IPKRQQPIAMSPSGGHFSITPFFSSLFNLLQFLGILFLALLSIELIGFLCLLGVKKERMI 125
           IPKRQQ I MSPS                                       GV+KERM 
Sbjct: 67  IPKRQQLILMSPS---------------------------------------GVQKERME 126

Query: 126 LEDIYVLSHSGSILYSPPPKPFSHKLP--NRCGPLFMK---------------------- 185
            ED+YVL+ +GSIL SP PKP+ +K P  + C PLF+K                      
Sbjct: 127 PEDMYVLATNGSILSSPSPKPYPYKPPKCSDCAPLFLKAYDMRNAGAVIHSHGMESCLVT 186

Query: 186 --GPIGS---------IKGISRDGF----------------------------------- 245
              P+           IKGI   G+                                   
Sbjct: 187 MINPLSKEFRITHMEMIKGIQGHGYYDELVVPIIENTAYENELTDSLAKAIEAYPKTTAV 246

Query: 246 -----GI-------------------------------------------NISVKAGAQG 305
                GI                                           N+ VKAG   
Sbjct: 247 LVRNHGIYIWGDSWISAKTQAECYHYLFDAAIKLHQIGLDWSTPNHGPIQNVKVKAGMNN 306

Query: 306 LDNKNEPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLSLTYETAETQDDIKLLRS 365
            +N+ EPLPRCIVLDIEGTTTPI+FV DVLFPYARDNVG+HLS TY+TAET+DDI LLR+
Sbjct: 307 SNNRIEPLPRCIVLDIEGTTTPITFVADVLFPYARDNVGRHLSATYDTAETKDDINLLRT 366

Query: 366 QVEDDLEKGVAGAVPVPPDNAAKEEVIAALVANVEAMIKADRKITALKQLQGHIWRTGFS 425
           QVEDDL +GV GA+P+P D+A KEEVIAALVANVEAMIKADRKITALKQLQGHIWRTG+ 
Sbjct: 367 QVEDDLAQGVDGAIPIPTDDAGKEEVIAALVANVEAMIKADRKITALKQLQGHIWRTGYE 426

Query: 426 GNELEGLVFDDVPEALKRWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTA 436
            NELEG+V+DDVPEAL++WHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDT 
Sbjct: 427 NNELEGVVYDDVPEALEKWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTT 486

[Close]

BLAST of Lag0019541 vs. ExPASy Swiss-Prot
Match: B9SQI7 (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Ricinus communis OX=3988 GN=RCOM_0591060 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 7.0e-136
Identity = 293/563 (52.04%), Postives = 330/563 (58.61%), Query Frame = 0


Query: 4   ASALVVN-GVKLASTSQAYLETKAANDTKALLSELYRQFYGVRSVSGAGGSIAIKVHHHS 63
           A A+ VN GVK+A  SQAYLE+KA  +T+ L+S+L RQFY +  VSG GGSI IKVH  S
Sbjct: 6   APAVAVNGGVKVA--SQAYLESKAVKETRVLISDLCRQFYNLGWVSGTGGSITIKVHDDS 65

Query: 64  IPKRQQPIAMSPSGGHFSITPFFSSLFNLLQFLGILFLALLSIELIGFLCLLGVKKERMI 123
           IPK  Q I MSPS                                       GV+KERM 
Sbjct: 66  IPKPNQLILMSPS---------------------------------------GVQKERME 125

Query: 124 LEDIYVLSHSGSILYSPPPKPFSHKLP--NRCGPLFMK---------------------- 183
            ED+YVL+ +GSIL SP PKP+ HK P  + CGPLF+K                      
Sbjct: 126 PEDMYVLAANGSILSSPSPKPYPHKPPKCSDCGPLFLKAYEMCNAGAVIHSHGMESCLVT 185

Query: 184 --GPIGS---------IKGISRDGF----------------------------------- 243
              P+           IKGI   G+                                   
Sbjct: 186 MINPLSKEFKITHMEMIKGIKGHGYYDELVVPIIENTAYENELTDSLAKAIEEYPKTTAV 245

Query: 244 ------------------------------------------------------------ 303
                                                                       
Sbjct: 246 LVRNHGIYIWGDSWISAKTQAECYHYLFDAAIKLHQLGLDWSTPNHGLIQNVKALIGSNR 305

Query: 304 GINISVKAGAQGLDNKNEPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLSLTYET 363
            IN SVKAG +  ++  + LP CIVLDIEGTTTPI+FV DVLFPYARDNVG+HL  TYET
Sbjct: 306 DINTSVKAGLKDSNHGMQSLPGCIVLDIEGTTTPITFVADVLFPYARDNVGRHLYATYET 365

Query: 364 AETQDDIKLLRSQVEDDLEKGVAGAVPVPPDNAAKEEVIAALVANVEAMIKADRKITALK 423
           AETQDDIKLLR+QVEDDL +GV  AV +PPD+A KEEVIAALVANVEAMIKADRKITALK
Sbjct: 366 AETQDDIKLLRTQVEDDLARGVNEAVSIPPDDAGKEEVIAALVANVEAMIKADRKITALK 425

Query: 424 QLQGHIWRTGFSGNELEGLVFDDVPEALKRWHALGIKVYIYSSGSRLAQRLIFGKTNYGD 436
           QLQGHIWRTGF  NELEG+V+DDVPEAL++WHALGIKVYIYSSGSRLAQRLIFGKTNYGD
Sbjct: 426 QLQGHIWRTGFQNNELEGVVYDDVPEALEKWHALGIKVYIYSSGSRLAQRLIFGKTNYGD 485

[Close]

BLAST of Lag0019541 vs. ExPASy Swiss-Prot
Match: E0CTF3 (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 2 OS=Vitis vinifera OX=29760 GN=VIT_12s0028g03470 PE=2 SV=2)

HSP 1 Score: 425.6 bits (1093), Expect = 6.6e-118
Identity = 252/559 (45.08%), Postives = 302/559 (54.03%), Query Frame = 0


Query: 9   VNGVKLASTSQ--AYLETKAANDTKALLSELYRQFYGVRSVSGAGGSIAIKVHHHSIPKR 68
           VNG ++ +T+    Y+ET+   + + L SEL R  Y +   SG GGSI IKVH  SIPK 
Sbjct: 12  VNGNEVGTTASFAGYMETRGVREARVLASELCRHMYTLGWFSGTGGSITIKVHDDSIPKP 71

Query: 69  QQPIAMSPSGGHFSITPFFSSLFNLLQFLGILFLALLSIELIGFLCLLGVKKERMILEDI 128
           +Q + +SPS                                       GV+KERM+ ED+
Sbjct: 72  RQLVVISPS---------------------------------------GVQKERMVPEDM 131

Query: 129 YVLSHSGSILYSPPPKPFSHKLP--NRCGPLFMK-------------------------- 188
           YVLS  G IL +PP KP+ HK P    C PLFMK                          
Sbjct: 132 YVLSSDGFILSTPPLKPYPHKPPKCTDCTPLFMKVYEMRDAGAVIHSHGMESCIVTMILP 191

Query: 189 ------------------------------------------------------------ 248
                                                                       
Sbjct: 192 FSKEFRITHMEMIKGIKGHGYHDELVVPIIENTAHEAELVESLTEAITAYPKTTAVLVRN 251

Query: 249 ------------------------------------------GPIGSIKGISRDGFGINI 308
                                                     GPI SI GI      ++ 
Sbjct: 252 HGVYVWGDSWISAKTQAECYHYLFDAAIKLHQLGLDWSTPTHGPIRSINGIWGCNGTMSR 311

Query: 309 SVKAGAQGLDNKNEPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLSLTYETAETQ 368
            +K G   LD+  EP  RCI+LDIEGTTTPISFVTDVLFPYA  NVGKHL+ T+++ ETQ
Sbjct: 312 GLKVGGLSLDDMIEPSQRCILLDIEGTTTPISFVTDVLFPYAHANVGKHLAATFDSEETQ 371

Query: 369 DDIKLLRSQVEDDLEKGVAGAVPVPPDNAAKEEVIAALVANVEAMIKADRKITALKQLQG 428
           DDI LLRSQ++ DLE GV GAVP+PPD   KE VIA+ VANVEAMI+ADR ITALKQLQG
Sbjct: 372 DDINLLRSQIQHDLEHGVVGAVPIPPDYVGKELVIASFVANVEAMIRADRNITALKQLQG 431

Query: 429 HIWRTGFSGNELEGLVFDDVPEALKRWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKY 436
           HIW+TGF  NEL G+VFDDVPEAL+RWHA GIKVYIYSSGSR AQ+LIF  +NYGDLRKY
Sbjct: 432 HIWKTGFQSNELVGVVFDDVPEALERWHASGIKVYIYSSGSREAQQLIFSNSNYGDLRKY 491

[Close]

BLAST of Lag0019541 vs. ExPASy Swiss-Prot
Match: Q9FN41 (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Arabidopsis thaliana OX=3702 GN=At5g53850 PE=1 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 4.3e-117
Identity = 251/546 (45.97%), Postives = 312/546 (57.14%), Query Frame = 0


Query: 7   LVVNGVKLASTSQAYLETKAANDTKALLSELYRQFYGVRSVSGAGGSIAIKVHHHSIPKR 66
           + V    +    QAYLE K   +T +L++EL R FY    VSG GGSI +KVH  SIPK 
Sbjct: 1   MAVAAAAMIGLPQAYLEGKEVKETSSLVTELCRHFYTQGWVSGTGGSITMKVHDASIPKP 60

Query: 67  QQPIAMSPSGGHFSITPFFSSLFNLLQFLGILFLALLSIELIGFLCLLGVKKERMILEDI 126
           +Q I MSPS                                       GV+KERM  ED+
Sbjct: 61  EQLIVMSPS---------------------------------------GVQKERMQPEDM 120

Query: 127 YVLSHSGSILYSPPPKPFSHKLP--NRCGPLFMKG------------------------- 186
           Y+LS +GSI+ +P PKP+ +K P    C PLFMK                          
Sbjct: 121 YILSANGSIISTPSPKPYPNKPPKCTDCAPLFMKAYEMRNAGAVIHSHGMESCLVTMLNP 180

Query: 187 --------PIGSIKGISRDGF--------------------------------------- 246
                    +  IKGI   G+                                       
Sbjct: 181 QAKEFRITHMEMIKGIQGHGYYDELVVPIIENTAYENELTDSLTKAIEAYPKATAVLVRN 240

Query: 247 -GINI---------------------SVKAGAQGLD---------------------NKN 306
            G+ I                     ++K    GLD                      + 
Sbjct: 241 HGVYIWGDSWIHAKTQAECYHYLFDAAIKLHQLGLDAATPDHGPIRRTIHSQIKDSQYER 300

Query: 307 EPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLSLTYETAETQDDIKLLRSQVEDD 366
           E   R IVLDIEGTTTPI+FVTDVLFPYAR+NVGKHL+LTY TAETQ+DIKLLR+QVE+D
Sbjct: 301 EWPRRWIVLDIEGTTTPITFVTDVLFPYARENVGKHLNLTYHTAETQEDIKLLRAQVEED 360

Query: 367 LEKGVAGAVPVPPDNAAKEEVIAALVANVEAMIKADRKITALKQLQGHIWRTGFSGNELE 426
           L +GV GAVP+P  +  KE+VIAA+V+NVEAMI+ADRKITALK+LQGHIWRTGF  +EL+
Sbjct: 361 LREGVTGAVPIPHADEGKEKVIAAMVSNVEAMIRADRKITALKELQGHIWRTGFECDELK 420

Query: 427 GLVFDDVPEALKRWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKR 436
            +VF+DV +AL++WH+ GIKVYIYSSGSRLAQ+L+FG T+YGDLRKY+SGFFDT +GNK+
Sbjct: 421 AIVFEDVADALEKWHSSGIKVYIYSSGSRLAQKLLFGNTDYGDLRKYISGFFDTTIGNKK 480

[Close]

BLAST of Lag0019541 vs. ExPASy TrEMBL
Match: A0A5D3CHG8 (Putative bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G00950 PE=4 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 3.3e-189
Identity = 365/466 (78.33%), Postives = 387/466 (83.05%), Query Frame = 0


Query: 1   MPMASALVVNGVKLASTSQAYLETKAANDTKALLSELYRQFYGVRSVSGAGGSIAIKVHH 60
           M MASAL ++GVKLA+TSQAY+ETKAANDTKALLSEL RQFY +R VSG G SIAIKVH 
Sbjct: 1   MAMASALPLSGVKLATTSQAYMETKAANDTKALLSELSRQFYTLRCVSGTGSSIAIKVHD 60

Query: 61  HSIPKRQQPIAMSPSGGHFSITPFFSSL----FNLLQFLGILFLALLSIELIGFLCLLGV 120
           HSIP+RQQ IAMS SGGH SI  F  S+    FN L FL IL L+LL IELIG +CLLGV
Sbjct: 61  HSIPRRQQLIAMSLSGGHLSILIFPLSVLVFPFNFLHFLAILLLSLLPIELIGSVCLLGV 120

Query: 121 KKERMILEDIYVLSHSGSILYSPPPKPFSHKLPN--RCGPLFMK---------------- 180
           KK RM+ ED Y+LS  GSIL+SPPPKPFSHKLPN     PLFMK                
Sbjct: 121 KKRRMVREDTYMLSSGGSILHSPPPKPFSHKLPNCFDSRPLFMKMRNAGAVIHSHSKESY 180

Query: 181 ---------GPIGSIKGISRDGFGINISVKAGAQGLDNKNEPLPRCIVLDIEGTTTPISF 240
                    G IGSIKGIS      NISVKAGA+GLDNKN+  PRCIVLDIEGTTTPISF
Sbjct: 181 LVTQRNHHQGQIGSIKGISS-----NISVKAGARGLDNKNQWFPRCIVLDIEGTTTPISF 240

Query: 241 VTDVLFPYARDNVGKHLSLTYETAETQDDIKLLRSQVEDDLEKGVAGAVPVPPDNAAKEE 300
           VTDVLFPYARDNV KHL LTYET ETQDDIKLLRSQVE+DLEKGVAGAVP+PPDNA KEE
Sbjct: 241 VTDVLFPYARDNVEKHLILTYETGETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEE 300

Query: 301 VIAALVANVEAMIKADRKITALKQLQGHIWRTGFSGNELEGLVFDDVPEALKRWHALGIK 360
           VIAA+VANVE MIKADRKITALKQLQGHIWRTGFSGNELEG+VFDDVPEAL+RWHA GIK
Sbjct: 301 VIAAVVANVEGMIKADRKITALKQLQGHIWRTGFSGNELEGVVFDDVPEALERWHASGIK 360

Query: 361 VYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREISSYVEISESVGVDTPSEI 420
           +YIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKRE SSYVEISESVGVD+PSEI
Sbjct: 361 MYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKRETSSYVEISESVGVDSPSEI 420

Query: 421 LFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTINSFSEI 436
           LFITDV QEA AAKAAGLQVAISIRPGNGPLPD+HGFQTI SFSEI
Sbjct: 421 LFITDVCQEAKAAKAAGLQVAISIRPGNGPLPDNHGFQTITSFSEI 461

[Close]

BLAST of Lag0019541 vs. ExPASy TrEMBL
Match: A0A5A7USH5 (Putative bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold803G00180 PE=4 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.8e-188
Identity = 364/466 (78.11%), Postives = 386/466 (82.83%), Query Frame = 0


Query: 1   MPMASALVVNGVKLASTSQAYLETKAANDTKALLSELYRQFYGVRSVSGAGGSIAIKVHH 60
           M MASAL ++GVKLA+TSQAY+ETKAANDTKALLSEL RQFY +R VSG G SIAIKVH 
Sbjct: 1   MAMASALPLSGVKLATTSQAYMETKAANDTKALLSELSRQFYTLRCVSGTGSSIAIKVHD 60

Query: 61  HSIPKRQQPIAMSPSGGHFSITPFFSSL----FNLLQFLGILFLALLSIELIGFLCLLGV 120
           HSIP+RQQ IAMS SGGH SI  F  S+    FN L FL IL L+LL IELIG +CLLGV
Sbjct: 61  HSIPRRQQLIAMSLSGGHLSILIFPLSVLVFPFNFLHFLAILLLSLLPIELIGSVCLLGV 120

Query: 121 KKERMILEDIYVLSHSGSILYSPPPKPFSHKLPN--RCGPLFMK---------------- 180
           KK RM+ ED Y+LS  GSIL+SPPPKPFSHKLPN     PLFMK                
Sbjct: 121 KKRRMVREDTYMLSSGGSILHSPPPKPFSHKLPNCFDSRPLFMKMRNAGAVIHSHSKESY 180

Query: 181 ---------GPIGSIKGISRDGFGINISVKAGAQGLDNKNEPLPRCIVLDIEGTTTPISF 240
                    G IGSIKGIS      NISVKAGA+GLDNKN+  PRCIVLDIEGTTTPISF
Sbjct: 181 LVTQRNHHQGQIGSIKGISS-----NISVKAGARGLDNKNQWFPRCIVLDIEGTTTPISF 240

Query: 241 VTDVLFPYARDNVGKHLSLTYETAETQDDIKLLRSQVEDDLEKGVAGAVPVPPDNAAKEE 300
           VTDVLF YARDNV KHL LTYET ETQDDIKLLRSQVE+DLEKGVAGAVP+PPDNA KEE
Sbjct: 241 VTDVLFSYARDNVEKHLILTYETGETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEE 300

Query: 301 VIAALVANVEAMIKADRKITALKQLQGHIWRTGFSGNELEGLVFDDVPEALKRWHALGIK 360
           VIAA+VANVE MIKADRKITALKQLQGHIWRTGFSGNELEG+VFDDVPEAL+RWHA GIK
Sbjct: 301 VIAAVVANVEGMIKADRKITALKQLQGHIWRTGFSGNELEGVVFDDVPEALERWHASGIK 360

Query: 361 VYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREISSYVEISESVGVDTPSEI 420
           +YIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKRE SSYVEISESVGVD+PSEI
Sbjct: 361 MYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKRETSSYVEISESVGVDSPSEI 420

Query: 421 LFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTINSFSEI 436
           LFITDV QEA AAKAAGLQVAISIRPGNGPLPD+HGFQTI SFSEI
Sbjct: 421 LFITDVCQEAKAAKAAGLQVAISIRPGNGPLPDNHGFQTITSFSEI 461

[Close]

BLAST of Lag0019541 vs. ExPASy TrEMBL
Match: A0A6J1E8E3 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111430820 PE=4 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 2.4e-179
Identity = 337/437 (77.12%), Postives = 359/437 (82.15%), Query Frame = 0


Query: 1   MPMASALVVNGVKLASTSQAYLETKAANDTKALLSELYRQFYGVRSVSGAGGSIAIKVHH 60
           M MAS L VNGVKLA+ S AY++TKAAN TKAL++EL RQFYG+R VSG  G I+IKVH 
Sbjct: 1   MAMASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHD 60

Query: 61  HSIPKRQQPIAMSPSGGHFSITPFFSSLFNLLQFLGILFLALLSIELIGFLCLLGVKKER 120
           HSIPK Q  +A+S S                                       GVKKER
Sbjct: 61  HSIPKGQHTVALSHS---------------------------------------GVKKER 120

Query: 121 MILEDIYVLSHSGSILYSPPPKPFSHKLPNRC--GPLFMKGPIGSIKGISRDGFGINISV 180
           M+ ED  +LS+ GSILYSPPPKPFSHKLP     GPLFMK P GSIKG+S       ISV
Sbjct: 121 MVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFMKDPFGSIKGMSS-----KISV 180

Query: 181 KAGAQGLDNKNEPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLSLTYETAETQDD 240
           KAGAQGLDN+N+PLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHL LTYETAETQDD
Sbjct: 181 KAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLILTYETAETQDD 240

Query: 241 IKLLRSQVEDDLEKGVAGAVPVPPDNAAKEEVIAALVANVEAMIKADRKITALKQLQGHI 300
           IKLLRSQVE+DLEKGVAGAVP+PPDNA KEEVIAALVANVEAMIKADRKITALKQLQGHI
Sbjct: 241 IKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMIKADRKITALKQLQGHI 300

Query: 301 WRTGFSGNELEGLVFDDVPEALKRWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLS 360
           WRTGF+GNELEG+VFDDVPEAL+RWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLS
Sbjct: 301 WRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLS 360

Query: 361 GFFDTAVGNKREISSYVEISESVGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNG 420
           GFFDTAVGNKREI SYVEISES+GVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNG
Sbjct: 361 GFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNG 393

Query: 421 PLPDDHGFQTINSFSEI 436
           PLPDDHGFQT  SFS+I
Sbjct: 421 PLPDDHGFQTATSFSDI 393

[Close]

BLAST of Lag0019541 vs. ExPASy TrEMBL
Match: A0A6J1EAE7 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430820 PE=4 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.6e-178
Identity = 335/434 (77.19%), Postives = 357/434 (82.26%), Query Frame = 0


Query: 4   ASALVVNGVKLASTSQAYLETKAANDTKALLSELYRQFYGVRSVSGAGGSIAIKVHHHSI 63
           AS L VNGVKLA+ S AY++TKAAN TKAL++EL RQFYG+R VSG  G I+IKVH HSI
Sbjct: 30  ASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHDHSI 89

Query: 64  PKRQQPIAMSPSGGHFSITPFFSSLFNLLQFLGILFLALLSIELIGFLCLLGVKKERMIL 123
           PK Q  +A+S S                                       GVKKERM+ 
Sbjct: 90  PKGQHTVALSHS---------------------------------------GVKKERMVA 149

Query: 124 EDIYVLSHSGSILYSPPPKPFSHKLPNRC--GPLFMKGPIGSIKGISRDGFGINISVKAG 183
           ED  +LS+ GSILYSPPPKPFSHKLP     GPLFMK P GSIKG+S       ISVKAG
Sbjct: 150 EDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFMKDPFGSIKGMSS-----KISVKAG 209

Query: 184 AQGLDNKNEPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLSLTYETAETQDDIKL 243
           AQGLDN+N+PLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHL LTYETAETQDDIKL
Sbjct: 210 AQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLILTYETAETQDDIKL 269

Query: 244 LRSQVEDDLEKGVAGAVPVPPDNAAKEEVIAALVANVEAMIKADRKITALKQLQGHIWRT 303
           LRSQVE+DLEKGVAGAVP+PPDNA KEEVIAALVANVEAMIKADRKITALKQLQGHIWRT
Sbjct: 270 LRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMIKADRKITALKQLQGHIWRT 329

Query: 304 GFSGNELEGLVFDDVPEALKRWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFF 363
           GF+GNELEG+VFDDVPEAL+RWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFF
Sbjct: 330 GFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFF 389

Query: 364 DTAVGNKREISSYVEISESVGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLP 423
           DTAVGNKREI SYVEISES+GVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLP
Sbjct: 390 DTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLP 419

Query: 424 DDHGFQTINSFSEI 436
           DDHGFQT  SFS+I
Sbjct: 450 DDHGFQTATSFSDI 419

[Close]

BLAST of Lag0019541 vs. ExPASy TrEMBL
Match: A0A6J1E4Z2 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430820 PE=4 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.6e-178
Identity = 335/434 (77.19%), Postives = 357/434 (82.26%), Query Frame = 0


Query: 4   ASALVVNGVKLASTSQAYLETKAANDTKALLSELYRQFYGVRSVSGAGGSIAIKVHHHSI 63
           AS L VNGVKLA+ S AY++TKAAN TKAL++EL RQFYG+R VSG  G I+IKVH HSI
Sbjct: 17  ASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHDHSI 76

Query: 64  PKRQQPIAMSPSGGHFSITPFFSSLFNLLQFLGILFLALLSIELIGFLCLLGVKKERMIL 123
           PK Q  +A+S S                                       GVKKERM+ 
Sbjct: 77  PKGQHTVALSHS---------------------------------------GVKKERMVA 136

Query: 124 EDIYVLSHSGSILYSPPPKPFSHKLPNRC--GPLFMKGPIGSIKGISRDGFGINISVKAG 183
           ED  +LS+ GSILYSPPPKPFSHKLP     GPLFMK P GSIKG+S       ISVKAG
Sbjct: 137 EDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFMKDPFGSIKGMSS-----KISVKAG 196

Query: 184 AQGLDNKNEPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLSLTYETAETQDDIKL 243
           AQGLDN+N+PLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHL LTYETAETQDDIKL
Sbjct: 197 AQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLILTYETAETQDDIKL 256

Query: 244 LRSQVEDDLEKGVAGAVPVPPDNAAKEEVIAALVANVEAMIKADRKITALKQLQGHIWRT 303
           LRSQVE+DLEKGVAGAVP+PPDNA KEEVIAALVANVEAMIKADRKITALKQLQGHIWRT
Sbjct: 257 LRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMIKADRKITALKQLQGHIWRT 316

Query: 304 GFSGNELEGLVFDDVPEALKRWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFF 363
           GF+GNELEG+VFDDVPEAL+RWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFF
Sbjct: 317 GFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFF 376

Query: 364 DTAVGNKREISSYVEISESVGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLP 423
           DTAVGNKREI SYVEISES+GVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLP
Sbjct: 377 DTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLP 406

Query: 424 DDHGFQTINSFSEI 436
           DDHGFQT  SFS+I
Sbjct: 437 DDHGFQTATSFSDI 406

[Close]

BLAST of Lag0019541 vs. TAIR 10
Match: AT5G53850.2 (haloacid dehalogenase-like hydrolase family protein )

HSP 1 Score: 422.9 bits (1086), Expect = 3.0e-118
Identity = 251/546 (45.97%), Postives = 312/546 (57.14%), Query Frame = 0


Query: 7   LVVNGVKLASTSQAYLETKAANDTKALLSELYRQFYGVRSVSGAGGSIAIKVHHHSIPKR 66
           + V    +    QAYLE K   +T +L++EL R FY    VSG GGSI +KVH  SIPK 
Sbjct: 1   MAVAAAAMIGLPQAYLEGKEVKETSSLVTELCRHFYTQGWVSGTGGSITMKVHDASIPKP 60

Query: 67  QQPIAMSPSGGHFSITPFFSSLFNLLQFLGILFLALLSIELIGFLCLLGVKKERMILEDI 126
           +Q I MSPS                                       GV+KERM  ED+
Sbjct: 61  EQLIVMSPS---------------------------------------GVQKERMQPEDM 120

Query: 127 YVLSHSGSILYSPPPKPFSHKLP--NRCGPLFMKG------------------------- 186
           Y+LS +GSI+ +P PKP+ +K P    C PLFMK                          
Sbjct: 121 YILSANGSIISTPSPKPYPNKPPKCTDCAPLFMKAYEMRNAGAVIHSHGMESCLVTMLNP 180

Query: 187 --------PIGSIKGISRDGF--------------------------------------- 246
                    +  IKGI   G+                                       
Sbjct: 181 QAKEFRITHMEMIKGIQGHGYYDELVVPIIENTAYENELTDSLTKAIEAYPKATAVLVRN 240

Query: 247 -GINI---------------------SVKAGAQGLD---------------------NKN 306
            G+ I                     ++K    GLD                      + 
Sbjct: 241 HGVYIWGDSWIHAKTQAECYHYLFDAAIKLHQLGLDAATPDHGPIRRTIHSQIKDSQYER 300

Query: 307 EPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLSLTYETAETQDDIKLLRSQVEDD 366
           E   R IVLDIEGTTTPI+FVTDVLFPYAR+NVGKHL+LTY TAETQ+DIKLLR+QVE+D
Sbjct: 301 EWPRRWIVLDIEGTTTPITFVTDVLFPYARENVGKHLNLTYHTAETQEDIKLLRAQVEED 360

Query: 367 LEKGVAGAVPVPPDNAAKEEVIAALVANVEAMIKADRKITALKQLQGHIWRTGFSGNELE 426
           L +GV GAVP+P  +  KE+VIAA+V+NVEAMI+ADRKITALK+LQGHIWRTGF  +EL+
Sbjct: 361 LREGVTGAVPIPHADEGKEKVIAAMVSNVEAMIRADRKITALKELQGHIWRTGFECDELK 420

Query: 427 GLVFDDVPEALKRWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKR 436
            +VF+DV +AL++WH+ GIKVYIYSSGSRLAQ+L+FG T+YGDLRKY+SGFFDT +GNK+
Sbjct: 421 AIVFEDVADALEKWHSSGIKVYIYSSGSRLAQKLLFGNTDYGDLRKYISGFFDTTIGNKK 480

[Close]

BLAST of Lag0019541 vs. TAIR 10
Match: AT5G53850.1 (haloacid dehalogenase-like hydrolase family protein )

HSP 1 Score: 256.9 bits (655), Expect = 2.9e-68
Identity = 173/440 (39.32%), Postives = 217/440 (49.32%), Query Frame = 0


Query: 7   LVVNGVKLASTSQAYLETKAANDTKALLSELYRQFYGVRSVSGAGGSIAIKVHHHSIPKR 66
           + V    +    QAYLE K   +T +L++EL R FY    VSG GGSI +KVH  SIPK 
Sbjct: 1   MAVAAAAMIGLPQAYLEGKEVKETSSLVTELCRHFYTQGWVSGTGGSITMKVHDASIPKP 60

Query: 67  QQPIAMSPSGGHFSITPFFSSLFNLLQFLGILFLALLSIELIGFLCLLGVKKERMILEDI 126
           +Q I MSPS                                       GV+KERM  ED+
Sbjct: 61  EQLIVMSPS---------------------------------------GVQKERMQPEDM 120

Query: 127 YVLSHSGSILYSPPPKPFSHKLP--NRCGPLFMKG------------------------- 186
           Y+LS +GSI+ +P PKP+ +K P    C PLFMK                          
Sbjct: 121 YILSANGSIISTPSPKPYPNKPPKCTDCAPLFMKAYEMRNAGAVIHSHGMESCLVTMLNP 180

Query: 187 --------PIGSIKGISRDGF--------------------------------------- 246
                    +  IKGI   G+                                       
Sbjct: 181 QAKEFRITHMEMIKGIQGHGYYDELVVPIIENTAYENELTDSLTKAIEAYPKATAVLVRN 240

Query: 247 -GINI---------------------SVKAGAQGLD---------------------NKN 306
            G+ I                     ++K    GLD                      + 
Sbjct: 241 HGVYIWGDSWIHAKTQAECYHYLFDAAIKLHQLGLDAATPDHGPIRRTIHSQIKDSQYER 300

Query: 307 EPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLSLTYETAETQDDIKLLRSQVEDD 330
           E   R IVLDIEGTTTPI+FVTDVLFPYAR+NVGKHL+LTY TAETQ+DIKLLR+QVE+D
Sbjct: 301 EWPRRWIVLDIEGTTTPITFVTDVLFPYARENVGKHLNLTYHTAETQEDIKLLRAQVEED 360

[Close]

BLAST of Lag0019541 vs. TAIR 10
Match: AT5G53850.3 (haloacid dehalogenase-like hydrolase family protein )

HSP 1 Score: 250.8 bits (639), Expect = 2.1e-66
Identity = 173/456 (37.94%), Postives = 217/456 (47.59%), Query Frame = 0


Query: 7   LVVNGVKLASTSQAYLETKAANDTKALLSELYRQFYGVRSVSGAGGSIAIKVHHHSIPKR 66
           + V    +    QAYLE K   +T +L++EL R FY    VSG GGSI +KVH  SIPK 
Sbjct: 1   MAVAAAAMIGLPQAYLEGKEVKETSSLVTELCRHFYTQGWVSGTGGSITMKVHDASIPKP 60

Query: 67  QQPIAMSPSGGHFSITPFFSSLFNLLQFLGILFLALLSIELIGFLCLLGVKKERMILEDI 126
           +Q I MSPS                                       GV+KERM  ED+
Sbjct: 61  EQLIVMSPS---------------------------------------GVQKERMQPEDM 120

Query: 127 YVLSHSGSILYSPPPKPFSHKLP--NRCGPLFMKG------------------------- 186
           Y+LS +GSI+ +P PKP+ +K P    C PLFMK                          
Sbjct: 121 YILSANGSIISTPSPKPYPNKPPKCTDCAPLFMKNRRLSLGKRLRKLGYKAYEMRNAGAV 180

Query: 187 ------------------------PIGSIKGISRDGF----------------------- 246
                                    +  IKGI   G+                       
Sbjct: 181 IHSHGMESCLVTMLNPQAKEFRITHMEMIKGIQGHGYYDELVVPIIENTAYENELTDSLT 240

Query: 247 -----------------GINI---------------------SVKAGAQGLD-------- 306
                            G+ I                     ++K    GLD        
Sbjct: 241 KAIEAYPKATAVLVRNHGVYIWGDSWIHAKTQAECYHYLFDAAIKLHQLGLDAATPDHGP 300

Query: 307 -------------NKNEPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLSLTYETA 330
                         + E   R IVLDIEGTTTPI+FVTDVLFPYAR+NVGKHL+LTY TA
Sbjct: 301 IRRTIHSQIKDSQYEREWPRRWIVLDIEGTTTPITFVTDVLFPYARENVGKHLNLTYHTA 360

The following BLAST results are available for this feature:

BLAST of Lag0019541 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK11241.1	6.9e-189	78.33	putative bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha...	[more]
KAA0058822.1	5.8e-188	78.11	putative bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha...	[more]
XP_038899594.1	8.7e-184	80.09	probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha...	[more]
XP_022923008.1	4.9e-179	77.12	probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha...	[more]
XP_023551888.1	3.2e-178	77.12	probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha...	[more]

BLAST of Lag0019541 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
E0CSI1	4.3e-141	52.79	Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha...	[more]
B9N1F9	1.3e-137	51.91	Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha...	[more]
B9SQI7	7.0e-136	52.04	Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha...	[more]
E0CTF3	6.6e-118	45.08	Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha...	[more]
Q9FN41	4.3e-117	45.97	Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha...	[more]

BLAST of Lag0019541 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CHG8	3.3e-189	78.33	Putative bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha...	[more]
A0A5A7USH5	2.8e-188	78.11	Putative bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha...	[more]
A0A6J1E8E3	2.4e-179	77.12	probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha...	[more]
A0A6J1EAE7	2.6e-178	77.19	probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha...	[more]
A0A6J1E4Z2	2.6e-178	77.19	probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha...	[more]

BLAST of Lag0019541 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53850.2	3.0e-118	45.97	haloacid dehalogenase-like hydrolase family protein	[more]
AT5G53850.1	2.9e-68	39.32	haloacid dehalogenase-like hydrolase family protein	[more]
AT5G53850.3	2.1e-66	37.94	haloacid dehalogenase-like hydrolase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.720.60		coord: 207..302 e-value: 4.2E-30 score: 106.5
None	No IPR available	SFLD	SFLDS00003	Haloacid_Dehalogenase	coord: 194..434 e-value: 0.0 score: 275.6
None	No IPR available	PANTHER	PTHR10640:SF8	BIFUNCTIONAL METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE/ENOLASE-PHOSPHATASE E1-RELATED	coord: 158..435 coord: 5..76
None	No IPR available	PANTHER	PTHR10640:SF8	BIFUNCTIONAL METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE/ENOLASE-PHOSPHATASE E1-RELATED	coord: 114..158
IPR023214	HAD superfamily	GENE3D	3.40.50.1000		coord: 303..435 e-value: 7.3E-47 score: 160.7
IPR023943	Enolase-phosphatase E1	TIGRFAM	TIGR01691	TIGR01691	coord: 194..434 e-value: 2.6E-68 score: 227.8
IPR023943	Enolase-phosphatase E1	SFLD	SFLDF00044	enolase-phosphatase	coord: 194..434 e-value: 0.0 score: 275.6
IPR023943	Enolase-phosphatase E1	CDD	cd01629	HAD_EP	coord: 195..416 e-value: 1.04549E-101 score: 299.458
IPR017714	Methylthioribulose-1-phosphate dehydratase	PANTHER	PTHR10640	METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE	coord: 5..76
IPR017714	Methylthioribulose-1-phosphate dehydratase	PANTHER	PTHR10640	METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE	coord: 158..435
IPR017714	Methylthioribulose-1-phosphate dehydratase	PANTHER	PTHR10640	METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE	coord: 114..158
IPR036412	HAD-like superfamily	SUPERFAMILY	56784	HAD-like	coord: 194..435

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0019541.1	Lag0019541.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016311	dephosphorylation
biological_process	GO:0019509	L-methionine salvage from methylthioadenosine
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0043874	acireductone synthase activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0046872	metal ion binding