Lag0019538 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0019538
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Description3-hydroxybutyryl-CoA dehydrogenase
Locationchr5: 43137823 .. 43140726 (+)
RNA-Seq ExpressionLag0019538
SyntenyLag0019538
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGGAAATCAAGGCCATCGGAGTTGTCGGCGGCGGCCAAATGGGCTCCGGAATTGCCCAACTCGCCGCCACACACGGCCACGAAGTTTGGCTAATCGATTCGGACTCTGCCGCTCTCGCTACAGCAACCAAATCCATTTCTTCCTCGATCCAACGCCTAGTCTCCAGAAACCTTCTCTCGCAAGTAAGAATCCTTACCATTCATTGCTTCGCCTCTAATTGCAAAGAATTTATTTGATATTTGGCCTGTGGTTGAACCCTGCTGATTTGGTTGATTGGGTATCTTGTTTTATAATTAAATGCGTTTTTTGATTAGTTTTGACTGGAGGGCTCTGCGTGTTAATGGATGTTTGAGTTATTTTGCCTTTTAATTATCATTTATTAGGAGTTTTGTGTTGTTTATTTCATAAGGTTGTTCTATGTAAAACCTCTTAACAGATCAATTACAGATCGGATCAGGGTGCATAAAGCAGGATTTAGTATTTTATTTGGTTGTTTACTATTATATTACATGGGCTTCCATCTCAAAACTAATTCGCAAGGAGTGGAGTGATCCTTAACATGCCCCTCAAATGGTGCCTCTTTGGGTTCACCATTTTTGGATCAGATCCCAATTTTTTTCTTTGGACCGAATACCCGTTTGAGTTTAATGGGCTCTCCACTAATCTTTTCAACGTGTGATCTTCAATATTTACTCATGAACATTATATAGCATCAGGAGATTTCAGTGTCTTGGCCTCTATTAGTACCCTTTCTGCTAAAGGGATAACACTGATTCACAGCTTTAGCAAAAAGAAAACGATAGAATCCTTTCCATATTCCATGTCTCTGCAGAGAAGCTTGTGATATAAATGGTTGGATGATGCCTGTTGCAGTACCAAACATGACAAATAGTGGGATTCTGAGCCAAAGCTTGGCTAAATCCACTTGATATTTGGAAAATAGATCATACAGATGATGTATTGGAATGGAGCCAATGAAACAAGAGTGGACTTTGATGAGCTTCTTTAGATGATGCATTGAACCCAAATCACTTTCTTATAGCTCTTCGGTACCTTTTTTTGCTATAGCAGCCTATGGCCTGTGATGCAAAATAACTGTTTCATTTTCATACTCATTGAGCACAATTTGATGTGTACAGCTAAACTGAGTTGATATTGATTATGGAACTGAACATACAACTTTAAAACAGAGCAGCTCTACCGTCAAAATGAACTTTCTCTGTTACTCTATTTTAATCAGATTCTGAAATCCACTTAACAGGAAGTTGGTGGCAGTGCTGTTCAGCGTTTAAAGTTTTCAACTGATTTGAAAGAGCTTCATTCAGCAGATGTCATCATCGAGGCTATCGTGGAATCAGAAGATGTTAAGAGAAAGCTGTTTCTTGAATTAGATAAGATTGCAAAACCATCTGCAATTCTAGCTTCTAATACAAGTTCCATCCCTATTACTCGCTTGGCCTCAGCAACCACACGTCCTGACCAGGTTTCGTTTTACAGTATATTCTATTAATATTAAACAATTCACTTTTTTTATTAATATTGCTCTTTGCTGCATTGGCCATTAAGCATCAAGTCCCCTACATGCGCCTGTTCTCGGGTTCGAGCCCGTCAAATCACCTTTTTAAATCGATTATTTTTTAATAATTGATTCTACAAGAAAAACTATAATTATTATCATTTCCATTTTTTTATTTATATTGAAAATAAGTTAAAGATACCATTTTTGTCTTTTATTTTGGGATTTGTTTCATTTTGGTCTACACATGTCCAAATTTAGTCTATGTACTTTAAGTAAATCTTAAAATTAGTACATATTGTTAGTATATTGTTAATGATTTTTTTTGTTTGTCTTTATTAAACCTTCTTCTATAAATTTTGGAAACATATTCACATCGTGTCTTTATTGAAATGGAAATTATTATCATTACTTTTACCAAATTTAATACAAATTTGTTTCAAACTACCAAAATAAGTACTAATTTTAAGATTTACTGAGAGTATATGGACTAAATTTGGACATTTAAAAGTACACATACTCAAATGTAATGAGACCTGCCTAAAGTATATTGACCAAAATTGTATTTTAACCTAAAAAAAATATCGATAATATTTTTCTTTTGAAGAAACTAATATCAAAATATATTAAGCTCCCTAACATAAAGATCTGTGCCAACAAGAGCATAGCTCAATTGGCATAAAGATGTATTGATAACCACGAAGTCTGTGGTTCGAATCCCCCACCCCTCACATTGTCAAAAAAAAATAATAATCTTATGCATCAACCTGCTGGTTTTCTCTTAAATACATTTGATGCCATTGCAGGTTATTGGCATGCATTTCATGAATCCCCCACCTATAATGAAGTTGGTCGAGATTGTTCGAGGCGCAGATACATCCGATAAAACATTTGATGCCATAAAAATCTTGGCAGAGAGGTGATACACTTCACTGATTAAGCATAGCAAATATAACGAAACATGTACCGATATTTATGTCGGTTTATGATGTCGTTACGAATGATGGTTTTCAGGCTTGGCAAAACAGTTATATGCTCTCAGGACTATTCTGGTTTCATTGTGAATAGAATCCTAATGCCAATGATTAACGAGGCGTTTTTCGCTCTCTACACGGGAGTGGCGACGAAGGAAGACGTGGATACAGGAATGAAGCTAGGAACTAATCATCCAATGGGTCCATTGGAGCTTGCAGACTTCATTGGTTTGGATGTCTGTTTATCAATCATGAAGGTTCTTCATTCTGGACTTGGTGATGATAAATATGCTCCCTGCCCTCTTCTTGTGCAGTATGTCGATGCGGGTCGCCTGGGACGAAAGCGAGGAATAGGCGTATACGATTACCGTAAACTAACACAACCAGGAAAATCTAAGTCTCGACTTTGA

mRNA sequence

ATGACGGAAATCAAGGCCATCGGAGTTGTCGGCGGCGGCCAAATGGGCTCCGGAATTGCCCAACTCGCCGCCACACACGGCCACGAAGTTTGGCTAATCGATTCGGACTCTGCCGCTCTCGCTACAGCAACCAAATCCATTTCTTCCTCGATCCAACGCCTAGTCTCCAGAAACCTTCTCTCGCAAGAAGTTGGTGGCAGTGCTGTTCAGCGTTTAAAGTTTTCAACTGATTTGAAAGAGCTTCATTCAGCAGATGTCATCATCGAGGCTATCGTGGAATCAGAAGATGTTAAGAGAAAGCTGTTTCTTGAATTAGATAAGATTGCAAAACCATCTGCAATTCTAGCTTCTAATACAAGTTCCATCCCTATTACTCGCTTGGCCTCAGCAACCACACGTCCTGACCAGGTTATTGGCATGCATTTCATGAATCCCCCACCTATAATGAAGTTGGTCGAGATTGTTCGAGGCGCAGATACATCCGATAAAACATTTGATGCCATAAAAATCTTGGCAGAGAGGCTTGGCAAAACAGTTATATGCTCTCAGGACTATTCTGGTTTCATTGTGAATAGAATCCTAATGCCAATGATTAACGAGGCGTTTTTCGCTCTCTACACGGGAGTGGCGACGAAGGAAGACGTGGATACAGGAATGAAGCTAGGAACTAATCATCCAATGGGTCCATTGGAGCTTGCAGACTTCATTGGTTTGGATGTCTGTTTATCAATCATGAAGGTTCTTCATTCTGGACTTGGTGATGATAAATATGCTCCCTGCCCTCTTCTTGTGCAGTATGTCGATGCGGGTCGCCTGGGACGAAAGCGAGGAATAGGCGTATACGATTACCGTAAACTAACACAACCAGGAAAATCTAAGTCTCGACTTTGA

Coding sequence (CDS)

ATGACGGAAATCAAGGCCATCGGAGTTGTCGGCGGCGGCCAAATGGGCTCCGGAATTGCCCAACTCGCCGCCACACACGGCCACGAAGTTTGGCTAATCGATTCGGACTCTGCCGCTCTCGCTACAGCAACCAAATCCATTTCTTCCTCGATCCAACGCCTAGTCTCCAGAAACCTTCTCTCGCAAGAAGTTGGTGGCAGTGCTGTTCAGCGTTTAAAGTTTTCAACTGATTTGAAAGAGCTTCATTCAGCAGATGTCATCATCGAGGCTATCGTGGAATCAGAAGATGTTAAGAGAAAGCTGTTTCTTGAATTAGATAAGATTGCAAAACCATCTGCAATTCTAGCTTCTAATACAAGTTCCATCCCTATTACTCGCTTGGCCTCAGCAACCACACGTCCTGACCAGGTTATTGGCATGCATTTCATGAATCCCCCACCTATAATGAAGTTGGTCGAGATTGTTCGAGGCGCAGATACATCCGATAAAACATTTGATGCCATAAAAATCTTGGCAGAGAGGCTTGGCAAAACAGTTATATGCTCTCAGGACTATTCTGGTTTCATTGTGAATAGAATCCTAATGCCAATGATTAACGAGGCGTTTTTCGCTCTCTACACGGGAGTGGCGACGAAGGAAGACGTGGATACAGGAATGAAGCTAGGAACTAATCATCCAATGGGTCCATTGGAGCTTGCAGACTTCATTGGTTTGGATGTCTGTTTATCAATCATGAAGGTTCTTCATTCTGGACTTGGTGATGATAAATATGCTCCCTGCCCTCTTCTTGTGCAGTATGTCGATGCGGGTCGCCTGGGACGAAAGCGAGGAATAGGCGTATACGATTACCGTAAACTAACACAACCAGGAAAATCTAAGTCTCGACTTTGA

Protein sequence

MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDSAALATATKSISSSIQRLVSRNLLSQEVGGSAVQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTSSIPITRLASATTRPDQVIGMHFMNPPPIMKLVEIVRGADTSDKTFDAIKILAERLGKTVICSQDYSGFIVNRILMPMINEAFFALYTGVATKEDVDTGMKLGTNHPMGPLELADFIGLDVCLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLTQPGKSKSRL
Homology
BLAST of Lag0019538 vs. NCBI nr
Match: XP_004146246.1 (uncharacterized protein LOC101221475 isoform X1 [Cucumis sativus])

HSP 1 Score: 535.0 bits (1377), Expect = 4.0e-148
Identity = 273/296 (92.23%), Postives = 289/296 (97.64%), Query Frame = 0

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDSAALATATKSISSSIQRLVSRNLL 60
           MTEIKAIGVVGGGQMGSGIAQLAAT+GH+VWLIDSDSAALA AT SISSSIQRLVSRNLL
Sbjct: 1   MTEIKAIGVVGGGQMGSGIAQLAATYGHQVWLIDSDSAALAKATDSISSSIQRLVSRNLL 60

Query: 61  SQEVGGSAVQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           S+E+G +A+QRLKFSTDLKELHSADVIIEAI+ESEDVKRKLF+ELDKIAK S+ILASNTS
Sbjct: 61  SREIGANALQRLKFSTDLKELHSADVIIEAILESEDVKRKLFVELDKIAKRSSILASNTS 120

Query: 121 SIPITRLASATTRPDQVIGMHFMNPPPIMKLVEIVRGADTSDKTFDAIKILAERLGKTVI 180
           SIPITRLASATTRP+QVIGMHFMNPPP+MKLVEIVRGADTS++TFDAIKILAERLGKTVI
Sbjct: 121 SIPITRLASATTRPEQVIGMHFMNPPPVMKLVEIVRGADTSNETFDAIKILAERLGKTVI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDVDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDYSGFIVNRILMPMINEAFFALYTGVATKED+DTGMKLGTNHPMGPLELADFIGLDV
Sbjct: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 240

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLTQPGKSKSRL 297
           CLSIMKVLHSGLGD+KYAPCPLLVQYVDAGRLGRKR IGVYDYRKL +P KSKSRL
Sbjct: 241 CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRRIGVYDYRKLPEPDKSKSRL 296

BLAST of Lag0019538 vs. NCBI nr
Match: XP_008456022.1 (PREDICTED: 3-hydroxybutyryl-CoA dehydrogenase [Cucumis melo] >KAA0058823.1 3-hydroxybutyryl-CoA dehydrogenase [Cucumis melo var. makuwa])

HSP 1 Score: 532.7 bits (1371), Expect = 2.0e-147
Identity = 274/296 (92.57%), Postives = 284/296 (95.95%), Query Frame = 0

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDSAALATATKSISSSIQRLVSRNLL 60
           MTEIKAIGVVGGGQMGSGIAQLAATHGH+VWLIDSD AALA AT SISSSIQRLVSRNLL
Sbjct: 14  MTEIKAIGVVGGGQMGSGIAQLAATHGHQVWLIDSDPAALAKATDSISSSIQRLVSRNLL 73

Query: 61  SQEVGGSAVQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           S+E+G  A+QRLKFSTDLKELHSADVIIEAIVESEDVKRKLF+ELDKIAK S+ILASNTS
Sbjct: 74  SREIGAIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFVELDKIAKRSSILASNTS 133

Query: 121 SIPITRLASATTRPDQVIGMHFMNPPPIMKLVEIVRGADTSDKTFDAIKILAERLGKTVI 180
           SIPITRLASATTRP+QVIGMHFMNPPPIMKLVEIVRGADTSD+TFDAIKILAERLGKTVI
Sbjct: 134 SIPITRLASATTRPEQVIGMHFMNPPPIMKLVEIVRGADTSDETFDAIKILAERLGKTVI 193

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDVDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDYSGFIVNRILMPMINEAFF LYTGVATKED+DTGMKLGTNHPMGPLELADFIGLDV
Sbjct: 194 CSQDYSGFIVNRILMPMINEAFFTLYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 253

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLTQPGKSKSRL 297
           CLSIMKVLHSGLGD+KYAPCPLLVQY DAGRLGRKRGIGVYDYRK    GKSKSRL
Sbjct: 254 CLSIMKVLHSGLGDNKYAPCPLLVQYADAGRLGRKRGIGVYDYRKQPLLGKSKSRL 309

BLAST of Lag0019538 vs. NCBI nr
Match: TYK11243.1 (3-hydroxybutyryl-CoA dehydrogenase [Cucumis melo var. makuwa])

HSP 1 Score: 532.3 bits (1370), Expect = 2.6e-147
Identity = 274/296 (92.57%), Postives = 285/296 (96.28%), Query Frame = 0

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDSAALATATKSISSSIQRLVSRNLL 60
           MTEIKAIGVVGGGQMGSGIAQLAATHGH+VWLIDSD AALA AT SISSSIQRLVSRNLL
Sbjct: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHQVWLIDSDPAALAKATDSISSSIQRLVSRNLL 60

Query: 61  SQEVGGSAVQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           S+E+G  A+QRLKFSTDLKELHSADVIIEAIVESEDVKRKLF+ELDKIAK S+ILASNTS
Sbjct: 61  SREIGAIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFVELDKIAKRSSILASNTS 120

Query: 121 SIPITRLASATTRPDQVIGMHFMNPPPIMKLVEIVRGADTSDKTFDAIKILAERLGKTVI 180
           SIPITRLASATTRP+QVIGMHFMNPPPIMKLVEIVRGADTS++TFDAIKILAERLGKTVI
Sbjct: 121 SIPITRLASATTRPEQVIGMHFMNPPPIMKLVEIVRGADTSNETFDAIKILAERLGKTVI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDVDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDYSGFIVNRILMPMINEAFF LYTGVATKED+DTGMKLGTNHPMGPLELADFIGLDV
Sbjct: 181 CSQDYSGFIVNRILMPMINEAFFTLYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 240

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLTQPGKSKSRL 297
           CLSIMKVLHSGLGD+KYAPCPLLVQYVDAGRLGRKRGIGVYDYRK    GKSKSRL
Sbjct: 241 CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKQPLLGKSKSRL 296

BLAST of Lag0019538 vs. NCBI nr
Match: XP_023553026.1 (uncharacterized protein LOC111810545 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 527.7 bits (1358), Expect = 6.4e-146
Identity = 270/296 (91.22%), Postives = 283/296 (95.61%), Query Frame = 0

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDSAALATATKSISSSIQRLVSRNLL 60
           MTEIK IGVVGGGQMGSGIAQLAA +GH+VWLIDSD AAL  AT SISSSIQRLVSRNLL
Sbjct: 1   MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNLL 60

Query: 61  SQEVGGSAVQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           S+EVG SA+QRLK STDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS
Sbjct: 61  SREVGVSALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120

Query: 121 SIPITRLASATTRPDQVIGMHFMNPPPIMKLVEIVRGADTSDKTFDAIKILAERLGKTVI 180
           SIPITRLAS+TTRPD+VIGMHFMNPPPIMKLVEIVRGADTSD+TF AIK LAERLGKT+I
Sbjct: 121 SIPITRLASSTTRPDKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDVDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDYSGFIVNRILMPMINEAFFALYTGVATKED+DTGMKLGTNHPMGPLELAD IGLDV
Sbjct: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLTQPGKSKSRL 297
           C+SIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRG+G+YDYRKL +P KSKSRL
Sbjct: 241 CVSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGIYDYRKLPKPEKSKSRL 296

BLAST of Lag0019538 vs. NCBI nr
Match: XP_038878166.1 (3-hydroxybutyryl-CoA dehydrogenase [Benincasa hispida])

HSP 1 Score: 527.3 bits (1357), Expect = 8.4e-146
Identity = 271/296 (91.55%), Postives = 284/296 (95.95%), Query Frame = 0

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDSAALATATKSISSSIQRLVSRNLL 60
           MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSD AALA A+ SISSSIQRL+S+NLL
Sbjct: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLISKNLL 60

Query: 61  SQEVGGSAVQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           S+EVG +A+QRLKFSTDLKELHSADVIIEAIVE EDVKRKLFLELDKIAK S+ILASNTS
Sbjct: 61  SREVGVNALQRLKFSTDLKELHSADVIIEAIVELEDVKRKLFLELDKIAKRSSILASNTS 120

Query: 121 SIPITRLASATTRPDQVIGMHFMNPPPIMKLVEIVRGADTSDKTFDAIKILAERLGKTVI 180
           SIPITRLASATTRP+QVIGMHFMNPPPIMKLVEI+RGADTSD+TFDAIKILAERLGKTVI
Sbjct: 121 SIPITRLASATTRPEQVIGMHFMNPPPIMKLVEIIRGADTSDETFDAIKILAERLGKTVI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDVDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDYSGFIVNRILMPMINEAFF LYTGVATKED+D GMKLGTN+PMGPLELADFIGLDV
Sbjct: 181 CSQDYSGFIVNRILMPMINEAFFTLYTGVATKEDIDKGMKLGTNYPMGPLELADFIGLDV 240

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLTQPGKSKSRL 297
           CLSIMKVLH GLGD+KYAPCPLLVQYVDAGRLGRKRGIGVYDYRKL Q  KSKSRL
Sbjct: 241 CLSIMKVLHCGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLPQSQKSKSRL 296

BLAST of Lag0019538 vs. ExPASy Swiss-Prot
Match: Q45223 (3-hydroxybutyryl-CoA dehydrogenase OS=Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) OX=224911 GN=hbdA PE=3 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.0e-82
Identity = 156/281 (55.52%), Postives = 202/281 (71.89%), Query Frame = 0

Query: 4   IKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDSAALATATKSISSSIQRLVSRNLLSQE 63
           IK +GV+G GQMG+GIA +AA  G +V L D  +  L +   +I+ ++ R VS+ ++++E
Sbjct: 5   IKKVGVIGAGQMGNGIAHVAALAGFDVVLNDVSADRLKSGMATINGNLARQVSKKVVTEE 64

Query: 64  VGGSAVQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTSSIP 123
               A+ R+  +  L +L   D++IE  VE E+VKRK+F EL  + KP AI+AS+TSSI 
Sbjct: 65  AKTKALSRIVAAEKLDDLADCDLVIETAVEKEEVKRKIFHELCAVLKPEAIVASDTSSIS 124

Query: 124 ITRLASATTRPDQVIGMHFMNPPPIMKLVEIVRGADTSDKTFDAIKILAERLGKTVICSQ 183
           ITRLA+AT RP++ IG+HFMNP P+M+LVE++RG  T D TF+A K    +LGK V  S+
Sbjct: 125 ITRLAAATDRPERFIGIHFMNPVPLMELVELIRGIATDDATFEASKEFVAKLGKQVAVSE 184

Query: 184 DYSGFIVNRILMPMINEAFFALYTGVATKEDVDTGMKLGTNHPMGPLELADFIGLDVCLS 243
           D+  FIVNRIL+PMINEA + LY GV   E +D  MKLG +HPMGPLELADFIGLD CLS
Sbjct: 185 DFPAFIVNRILLPMINEAIYTLYEGVGNVEAIDAAMKLGAHHPMGPLELADFIGLDTCLS 244

Query: 244 IMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGIGVYDYR 285
           IM+VLH GL D KY PCPLLV+YV+AG LGRK   G YDYR
Sbjct: 245 IMQVLHEGLADSKYRPCPLLVKYVEAGWLGRKTQRGFYDYR 285

BLAST of Lag0019538 vs. ExPASy Swiss-Prot
Match: P45856 (Probable 3-hydroxybutyryl-CoA dehydrogenase OS=Bacillus subtilis (strain 168) OX=224308 GN=mmgB PE=2 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 3.1e-79
Identity = 152/285 (53.33%), Postives = 192/285 (67.37%), Query Frame = 0

Query: 3   EIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDSAALATATKSISSSIQRLVSRNLLSQ 62
           EIK I V G GQMGSGIAQ AA  G  V + D +  A     K +   + R   +   ++
Sbjct: 2   EIKQIMVAGAGQMGSGIAQTAADAGFYVRMYDVNPEAAEAGLKRLKKQLARDAEKGKRTE 61

Query: 63  EVGGSAVQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTSSI 122
               S + R+  S  L+E   AD++IEAI E+   K ++F  LD+I  P  ILASNTSS+
Sbjct: 62  TEVKSVINRISISQTLEEAEHADIVIEAIAENMAAKTEMFKTLDRICPPHTILASNTSSL 121

Query: 123 PITRLASATTRPDQVIGMHFMNPPPIMKLVEIVRGADTSDKTFDAIKILAERLGKTVICS 182
           PIT +A+ T RP +VIGMHFMNP P+MKLVE++RG  TS++T   +  LAE++GKT +  
Sbjct: 122 PITEIAAVTNRPQRVIGMHFMNPVPVMKLVEVIRGLATSEETALDVMALAEKMGKTAVEV 181

Query: 183 QDYSGFIVNRILMPMINEAFFALYTGVATKEDVDTGMKLGTNHPMGPLELADFIGLDVCL 242
            D+ GF+ NR+L+PMINEA + +Y GVA  E +D  MKLG NHPMGPL LADFIGLD CL
Sbjct: 182 NDFPGFVSNRVLLPMINEAIYCVYEGVAKPEAIDEVMKLGMNHPMGPLALADFIGLDTCL 241

Query: 243 SIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLT 288
           SIM+VLHSGLGD KY PCPLL +YV AG LG+K G G YDY + T
Sbjct: 242 SIMEVLHSGLGDSKYRPCPLLRKYVKAGWLGKKSGRGFYDYEEKT 286

BLAST of Lag0019538 vs. ExPASy Swiss-Prot
Match: Q9RVG1 (Probable 3-hydroxybutyryl-CoA dehydrogenase OS=Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) OX=243230 GN=hbd PE=3 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 8.0e-75
Identity = 148/276 (53.62%), Postives = 189/276 (68.48%), Query Frame = 0

Query: 8   GVVGGGQMGSGIAQLAATHGHEVWLIDSDSAALATATKSISSSIQRLVSRNLLSQEVGGS 67
           GV+G GQMG GIAQ+AA  G +V + D     L      I  S+ +L  +  L+ +   +
Sbjct: 4   GVIGAGQMGGGIAQVAAQSGFDVVVHDQKQEFLDRGKGVIEKSLGKLHEKGKLT-DAPET 63

Query: 68  AVQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTSSIPITRL 127
            + R+ F+TDL++    D+++EAIVE++ +K  LF +L +I KP  ILASNTSSIPIT L
Sbjct: 64  VLGRIHFTTDLQDFADCDLVVEAIVENQQIKNDLFKQLGQIVKPEGILASNTSSIPITAL 123

Query: 128 ASATTRPDQVIGMHFMNPPPIMKLVEIVRGADTSDKTFDAIKILAERLGKTVICSQDYSG 187
           A+A+ RP Q IGMHFMNP P+M+LVE++RG  TSD+T   +   AE+LGKT +   D+ G
Sbjct: 124 ATASGRPAQFIGMHFMNPVPLMQLVEVIRGYQTSDETARIVTETAEKLGKTPLSCNDFPG 183

Query: 188 FIVNRILMPMINEAFFALYTGVATKEDVDTGMKLGTNHPMGPLELADFIGLDVCLSIMKV 247
           F+ NRILMPM+NEA   +  GVA  E +D  MKLG NHPMGPL LADFIGLD CL+IM+V
Sbjct: 184 FVSNRILMPMLNEAIQCVMEGVAEPEAIDGIMKLGMNHPMGPLTLADFIGLDTCLAIMEV 243

Query: 248 LHSGLGDDKYAPCPLLVQYVDAGRLGRKRGIGVYDY 284
           LH GLGDDKY P PLL + V AG LGRK G G Y Y
Sbjct: 244 LHQGLGDDKYRPSPLLRKMVQAGLLGRKSGEGFYKY 278

BLAST of Lag0019538 vs. ExPASy Swiss-Prot
Match: P52041 (3-hydroxybutyryl-CoA dehydrogenase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=hbd PE=1 SV=2)

HSP 1 Score: 277.3 bits (708), Expect = 2.0e-73
Identity = 145/282 (51.42%), Postives = 187/282 (66.31%), Query Frame = 0

Query: 4   IKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDSAALATATKSISSSIQRLVSRNLLSQE 63
           +K + V+G G MGSGIAQ  A  G EV L D     +      I+ ++ +LV +  + + 
Sbjct: 1   MKKVCVIGAGTMGSGIAQAFAAKGFEVVLRDIKDEFVDRGLDFINKNLSKLVKKGKIEEA 60

Query: 64  VGGSAVQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTSSIP 123
                + R+  + DL      D++IEA VE  D+K+++F +LD I KP  ILASNTSS+ 
Sbjct: 61  TKVEILTRISGTVDLNMAADCDLVIEAAVERMDIKKQIFADLDNICKPETILASNTSSLS 120

Query: 124 ITRLASATTRPDQVIGMHFMNPPPIMKLVEIVRGADTSDKTFDAIKILAERLGKTVICSQ 183
           IT +ASAT RPD+VIGMHF NP P+MKLVE++RG  TS +TFDA+K  +  +GK  +   
Sbjct: 121 ITEVASATKRPDKVIGMHFFNPAPVMKLVEVIRGIATSQETFDAVKETSIAIGKDPVEVA 180

Query: 184 DYSGFIVNRILMPMINEAFFALYTGVATKEDVDTGMKLGTNHPMGPLELADFIGLDVCLS 243
           +  GF+VNRIL+PMINEA   L  G+A+ ED+D  MKLG NHPMGPLEL DFIGLD+CL+
Sbjct: 181 EAPGFVVNRILIPMINEAVGILAEGIASVEDIDKAMKLGANHPMGPLELGDFIGLDICLA 240

Query: 244 IMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGIGVYDYRK 286
           IM VL+S  GD KY P  LL +YV AG LGRK G G YDY K
Sbjct: 241 IMDVLYSETGDSKYRPHTLLKKYVRAGWLGRKSGKGFYDYSK 282

BLAST of Lag0019538 vs. ExPASy Swiss-Prot
Match: P77851 (3-hydroxybutyryl-CoA dehydrogenase OS=Thermoanaerobacterium thermosaccharolyticum (strain ATCC 7956 / DSM 571 / NCIMB 9385 / NCA 3814 / NCTC 13789 / WDCM 00135 / 2032) OX=580327 GN=hbd PE=3 SV=2)

HSP 1 Score: 265.4 bits (677), Expect = 7.8e-70
Identity = 137/281 (48.75%), Postives = 194/281 (69.04%), Query Frame = 0

Query: 4   IKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDSAALATATKSISSSIQRLVSRNLLSQE 63
           ++ I V+G G MGSGIAQ+ A +G EV L D D   +     +I  ++QR V +  ++ +
Sbjct: 1   MQKICVIGAGTMGSGIAQVFAQNGFEVILRDIDMKFVEKGFGTIEKNLQRNVDKGKITAD 60

Query: 64  VGGSAVQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTSSIP 123
                + R++ +T+L++   AD ++EA +E+ D+K+++F ELD+I K   ILASNTSS+ 
Sbjct: 61  EKNEILSRIRGTTNLEDAKEADFVVEAAIENMDLKKQIFKELDEICKMETILASNTSSLS 120

Query: 124 ITRLASATTRPDQVIGMHFMNPPPIMKLVEIVRGADTSDKTFDAIKILAERLGKTVICSQ 183
           IT +ASAT RP++VIGMHF NP P+MKLVE+++G  TS++TF+ ++ LA ++ KT I  +
Sbjct: 121 ITEIASATKRPEKVIGMHFFNPVPVMKLVEVIKGLKTSEQTFNVVRELALKVDKTPIEVK 180

Query: 184 DYSGFIVNRILMPMINEAFFALYTGVATKEDVDTGMKLGTNHPMGPLELADFIGLDVCLS 243
           +  GF+VNRIL+PMINEA   L  GVAT ED+D  MKLG NHP+GPL L+D IG DV L+
Sbjct: 181 EAPGFVVNRILIPMINEAIGILADGVATAEDIDEAMKLGANHPIGPLALSDLIGNDVVLA 240

Query: 244 IMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGIGVYDYR 285
           IM VL+   GD KY P PLL + V  G LGRK G G ++Y+
Sbjct: 241 IMNVLYEEYGDSKYRPHPLLKKMVRGGLLGRKTGKGFFEYK 281

BLAST of Lag0019538 vs. ExPASy TrEMBL
Match: A0A0A0L6N5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G234000 PE=3 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 1.9e-148
Identity = 273/296 (92.23%), Postives = 289/296 (97.64%), Query Frame = 0

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDSAALATATKSISSSIQRLVSRNLL 60
           MTEIKAIGVVGGGQMGSGIAQLAAT+GH+VWLIDSDSAALA AT SISSSIQRLVSRNLL
Sbjct: 1   MTEIKAIGVVGGGQMGSGIAQLAATYGHQVWLIDSDSAALAKATDSISSSIQRLVSRNLL 60

Query: 61  SQEVGGSAVQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           S+E+G +A+QRLKFSTDLKELHSADVIIEAI+ESEDVKRKLF+ELDKIAK S+ILASNTS
Sbjct: 61  SREIGANALQRLKFSTDLKELHSADVIIEAILESEDVKRKLFVELDKIAKRSSILASNTS 120

Query: 121 SIPITRLASATTRPDQVIGMHFMNPPPIMKLVEIVRGADTSDKTFDAIKILAERLGKTVI 180
           SIPITRLASATTRP+QVIGMHFMNPPP+MKLVEIVRGADTS++TFDAIKILAERLGKTVI
Sbjct: 121 SIPITRLASATTRPEQVIGMHFMNPPPVMKLVEIVRGADTSNETFDAIKILAERLGKTVI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDVDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDYSGFIVNRILMPMINEAFFALYTGVATKED+DTGMKLGTNHPMGPLELADFIGLDV
Sbjct: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 240

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLTQPGKSKSRL 297
           CLSIMKVLHSGLGD+KYAPCPLLVQYVDAGRLGRKR IGVYDYRKL +P KSKSRL
Sbjct: 241 CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRRIGVYDYRKLPEPDKSKSRL 296

BLAST of Lag0019538 vs. ExPASy TrEMBL
Match: A0A5A7USC9 (3-hydroxybutyryl-CoA dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold803G00200 PE=3 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 9.6e-148
Identity = 274/296 (92.57%), Postives = 284/296 (95.95%), Query Frame = 0

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDSAALATATKSISSSIQRLVSRNLL 60
           MTEIKAIGVVGGGQMGSGIAQLAATHGH+VWLIDSD AALA AT SISSSIQRLVSRNLL
Sbjct: 14  MTEIKAIGVVGGGQMGSGIAQLAATHGHQVWLIDSDPAALAKATDSISSSIQRLVSRNLL 73

Query: 61  SQEVGGSAVQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           S+E+G  A+QRLKFSTDLKELHSADVIIEAIVESEDVKRKLF+ELDKIAK S+ILASNTS
Sbjct: 74  SREIGAIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFVELDKIAKRSSILASNTS 133

Query: 121 SIPITRLASATTRPDQVIGMHFMNPPPIMKLVEIVRGADTSDKTFDAIKILAERLGKTVI 180
           SIPITRLASATTRP+QVIGMHFMNPPPIMKLVEIVRGADTSD+TFDAIKILAERLGKTVI
Sbjct: 134 SIPITRLASATTRPEQVIGMHFMNPPPIMKLVEIVRGADTSDETFDAIKILAERLGKTVI 193

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDVDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDYSGFIVNRILMPMINEAFF LYTGVATKED+DTGMKLGTNHPMGPLELADFIGLDV
Sbjct: 194 CSQDYSGFIVNRILMPMINEAFFTLYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 253

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLTQPGKSKSRL 297
           CLSIMKVLHSGLGD+KYAPCPLLVQY DAGRLGRKRGIGVYDYRK    GKSKSRL
Sbjct: 254 CLSIMKVLHSGLGDNKYAPCPLLVQYADAGRLGRKRGIGVYDYRKQPLLGKSKSRL 309

BLAST of Lag0019538 vs. ExPASy TrEMBL
Match: A0A1S3C2Y7 (3-hydroxybutyryl-CoA dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103496074 PE=3 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 9.6e-148
Identity = 274/296 (92.57%), Postives = 284/296 (95.95%), Query Frame = 0

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDSAALATATKSISSSIQRLVSRNLL 60
           MTEIKAIGVVGGGQMGSGIAQLAATHGH+VWLIDSD AALA AT SISSSIQRLVSRNLL
Sbjct: 14  MTEIKAIGVVGGGQMGSGIAQLAATHGHQVWLIDSDPAALAKATDSISSSIQRLVSRNLL 73

Query: 61  SQEVGGSAVQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           S+E+G  A+QRLKFSTDLKELHSADVIIEAIVESEDVKRKLF+ELDKIAK S+ILASNTS
Sbjct: 74  SREIGAIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFVELDKIAKRSSILASNTS 133

Query: 121 SIPITRLASATTRPDQVIGMHFMNPPPIMKLVEIVRGADTSDKTFDAIKILAERLGKTVI 180
           SIPITRLASATTRP+QVIGMHFMNPPPIMKLVEIVRGADTSD+TFDAIKILAERLGKTVI
Sbjct: 134 SIPITRLASATTRPEQVIGMHFMNPPPIMKLVEIVRGADTSDETFDAIKILAERLGKTVI 193

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDVDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDYSGFIVNRILMPMINEAFF LYTGVATKED+DTGMKLGTNHPMGPLELADFIGLDV
Sbjct: 194 CSQDYSGFIVNRILMPMINEAFFTLYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 253

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLTQPGKSKSRL 297
           CLSIMKVLHSGLGD+KYAPCPLLVQY DAGRLGRKRGIGVYDYRK    GKSKSRL
Sbjct: 254 CLSIMKVLHSGLGDNKYAPCPLLVQYADAGRLGRKRGIGVYDYRKQPLLGKSKSRL 309

BLAST of Lag0019538 vs. ExPASy TrEMBL
Match: A0A5D3CH80 (3-hydroxybutyryl-CoA dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G00980 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.3e-147
Identity = 274/296 (92.57%), Postives = 285/296 (96.28%), Query Frame = 0

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDSAALATATKSISSSIQRLVSRNLL 60
           MTEIKAIGVVGGGQMGSGIAQLAATHGH+VWLIDSD AALA AT SISSSIQRLVSRNLL
Sbjct: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHQVWLIDSDPAALAKATDSISSSIQRLVSRNLL 60

Query: 61  SQEVGGSAVQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           S+E+G  A+QRLKFSTDLKELHSADVIIEAIVESEDVKRKLF+ELDKIAK S+ILASNTS
Sbjct: 61  SREIGAIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFVELDKIAKRSSILASNTS 120

Query: 121 SIPITRLASATTRPDQVIGMHFMNPPPIMKLVEIVRGADTSDKTFDAIKILAERLGKTVI 180
           SIPITRLASATTRP+QVIGMHFMNPPPIMKLVEIVRGADTS++TFDAIKILAERLGKTVI
Sbjct: 121 SIPITRLASATTRPEQVIGMHFMNPPPIMKLVEIVRGADTSNETFDAIKILAERLGKTVI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDVDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDYSGFIVNRILMPMINEAFF LYTGVATKED+DTGMKLGTNHPMGPLELADFIGLDV
Sbjct: 181 CSQDYSGFIVNRILMPMINEAFFTLYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 240

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLTQPGKSKSRL 297
           CLSIMKVLHSGLGD+KYAPCPLLVQYVDAGRLGRKRGIGVYDYRK    GKSKSRL
Sbjct: 241 CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKQPLLGKSKSRL 296

BLAST of Lag0019538 vs. ExPASy TrEMBL
Match: A0A6J1J8K2 (uncharacterized protein LOC111482724 OS=Cucurbita maxima OX=3661 GN=LOC111482724 PE=3 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 1.3e-144
Identity = 265/296 (89.53%), Postives = 281/296 (94.93%), Query Frame = 0

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDSAALATATKSISSSIQRLVSRNLL 60
           MTEIK IGV+GGGQMGSGIAQLAA +GH++WLIDSD AAL  AT SISSSIQRLVSRNLL
Sbjct: 1   MTEIKVIGVIGGGQMGSGIAQLAAAYGHDIWLIDSDPAALTKATNSISSSIQRLVSRNLL 60

Query: 61  SQEVGGSAVQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           S+EVG  A+QRLK STDLKELHSADVIIEA+VESEDVKRKLFLELDKIAKPSAILASNTS
Sbjct: 61  SREVGVDALQRLKCSTDLKELHSADVIIEAVVESEDVKRKLFLELDKIAKPSAILASNTS 120

Query: 121 SIPITRLASATTRPDQVIGMHFMNPPPIMKLVEIVRGADTSDKTFDAIKILAERLGKTVI 180
           SIPITRLAS+TTRP++VIGMHFMNPPPIMKLVEIVRG DTSD+TFDAIK LAERLGKT+I
Sbjct: 121 SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGVDTSDETFDAIKTLAERLGKTLI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDVDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDYSGFIVNR+LMPMINEAFFALYTGVATKED+DTGMKLGTNHPMGPLELAD IGLDV
Sbjct: 181 CSQDYSGFIVNRLLMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLTQPGKSKSRL 297
           CLSIMKVL SGLGDDKYAPCPLLVQYVDAGRLGRKRG+GVYDYRKL +P KSKSRL
Sbjct: 241 CLSIMKVLQSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYDYRKLPKPEKSKSRL 296

BLAST of Lag0019538 vs. TAIR 10
Match: AT3G15290.1 (3-hydroxyacyl-CoA dehydrogenase family protein )

HSP 1 Score: 444.5 bits (1142), Expect = 6.6e-125
Identity = 219/288 (76.04%), Postives = 256/288 (88.89%), Query Frame = 0

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDSAALATATKSISSSIQRLVSRNLL 60
           M E+K++GVVG GQMGSGIAQLAAT G +VWL+D+D  AL+ AT +ISSS++R VS+ L+
Sbjct: 1   MAEMKSVGVVGAGQMGSGIAQLAATSGLDVWLMDADRDALSRATAAISSSVKRFVSKGLI 60

Query: 61  SQEVGGSAVQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           S+EVG  A+ RL+ +++L++L SAD+I+EAIVESED+K+KLF +LD IAK SAILASNTS
Sbjct: 61  SKEVGDDAMHRLRLTSNLEDLCSADIIVEAIVESEDIKKKLFKDLDGIAKSSAILASNTS 120

Query: 121 SIPITRLASATTRPDQVIGMHFMNPPPIMKLVEIVRGADTSDKTFDAIKILAERLGKTVI 180
           SI ITRLASAT RP QVIGMHFMNPPPIMKLVEI+RGADTS++TF A K+LAER GKT +
Sbjct: 121 SISITRLASATRRPSQVIGMHFMNPPPIMKLVEIIRGADTSEETFLATKVLAERFGKTTV 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDVDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDY+GF+VNRILMPMINEAF  LYTGVATKED+D+GMK GTNHPMGPLELAD IGLDV
Sbjct: 181 CSQDYAGFVVNRILMPMINEAFHTLYTGVATKEDIDSGMKHGTNHPMGPLELADLIGLDV 240

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLTQ 289
           CLS+MKVLH GLGD KYAPCPLLVQYVDAGRLGRKRG+GVYDYR+ TQ
Sbjct: 241 CLSVMKVLHEGLGDSKYAPCPLLVQYVDAGRLGRKRGVGVYDYREATQ 288

BLAST of Lag0019538 vs. TAIR 10
Match: AT4G29010.1 (Enoyl-CoA hydratase/isomerase family )

HSP 1 Score: 147.9 bits (372), Expect = 1.3e-35
Identity = 95/295 (32.20%), Postives = 150/295 (50.85%), Query Frame = 0

Query: 4   IKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDSAALATATKSISSSIQRLVSRNLLSQE 63
           IK + V+GGG MGSGIA         V L + +S  L    KS+ ++++ LVSR  L+Q+
Sbjct: 309 IKKVAVIGGGLMGSGIATALLLSNIRVVLKEINSEFLMKGIKSVEANMKSLVSRGKLTQD 368

Query: 64  VGGSAVQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTSSIP 123
             G A+   K   D  E +  D++IEA++E+  +K+ +F E++K+  P  ILASNTS+I 
Sbjct: 369 KAGKALSLFKGVLDYTEFNDVDMVIEAVIENIQLKQNIFKEIEKVCSPHCILASNTSTID 428

Query: 124 ITRLASATTRPDQVIGMHFMNPPPIMKLVEIVRGADTSDKTFDAIKILAERLGKTVICSQ 183
           +  +   T   D+++G HF +P  +M L+EIVR  +TS +    +  + + + K  +   
Sbjct: 429 LDVIGEKTNSKDRIVGAHFFSPAHLMPLLEIVRSKNTSAQVILDLMAVGKAIKKVPVVVG 488

Query: 184 DYSGFIVNRILMPMINEAFFALYTGV--ATKEDVDTGMKLGTNHPMGPLELADFIGLDVC 243
           +  GF VNR   P    A      GV     + V T   L    P+GP +L D  G  + 
Sbjct: 489 NCIGFAVNRTFFPYSQAAHMLANLGVDLFRIDSVITSFGL----PLGPFQLGDLAGHGIG 548

Query: 244 LSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLTQPGKSKSRL 297
           L++  +     GD  +   P+    + +GR G+  G G Y Y K ++P    S L
Sbjct: 549 LAVGPIYAKVYGDRMFR-SPMTELLLKSGRNGKINGRGYYIYEKGSKPKPDPSVL 598

BLAST of Lag0019538 vs. TAIR 10
Match: AT3G06860.1 (multifunctional protein 2 )

HSP 1 Score: 137.9 bits (346), Expect = 1.3e-32
Identity = 89/290 (30.69%), Postives = 147/290 (50.69%), Query Frame = 0

Query: 3   EIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDSAALATATKSISSSIQRLVSRNLLSQ 62
           +IK + ++GGG MGSGIA       + V L + +   L      + +++Q  V +  +SQ
Sbjct: 311 KIKKVAIIGGGLMGSGIATALILSNYPVILKEVNEKFLEAGIGRVKANLQSRVRKGSMSQ 370

Query: 63  EVGGSAVQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTSSI 122
           E     +  LK S D +     D++IEA++E+  +K+++F +L+K      ILASNTS+I
Sbjct: 371 EKFEKTMSLLKGSLDYESFRDVDMVIEAVIENISLKQQIFADLEKYCPQHCILASNTSTI 430

Query: 123 PITRLASATTRPDQVIGMHFMNPPPIMKLVEIVRGADTSDKTFDAIKILAERLGKTVICS 182
            + ++   T   D+++G HF +P  IM L+EIVR   TS +    +  + +++ KT +  
Sbjct: 431 DLNKIGERTKSQDRIVGAHFFSPAHIMPLLEIVRTNHTSAQVIVDLLDVGKKIKKTPVVV 490

Query: 183 QDYSGFIVNRILMPMINEAFFALYTGVATKEDVDTGM-KLGTNHPMGPLELADFIGLDVC 242
            + +GF VNR+  P    A F +  G A    +D  + K G   PMGP  L D +G  V 
Sbjct: 491 GNCTGFAVNRMFFPYTQAAMFLVECG-ADPYLIDRAISKFGM--PMGPFRLCDLVGFGVA 550

Query: 243 LSIMKVLHSGLGDDKYAP--CPLLVQYVDAGRLGRKRGIGVYDYRKLTQP 290
           ++          +  Y     PL+ +   AG   RK G  +YD ++  +P
Sbjct: 551 IATATQFIENFSERTYKSMIIPLMQEDKRAGEATRK-GFYLYDDKRKAKP 596

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004146246.14.0e-14892.23uncharacterized protein LOC101221475 isoform X1 [Cucumis sativus][more]
XP_008456022.12.0e-14792.57PREDICTED: 3-hydroxybutyryl-CoA dehydrogenase [Cucumis melo] >KAA0058823.1 3-hyd... [more]
TYK11243.12.6e-14792.573-hydroxybutyryl-CoA dehydrogenase [Cucumis melo var. makuwa][more]
XP_023553026.16.4e-14691.22uncharacterized protein LOC111810545 [Cucurbita pepo subsp. pepo][more]
XP_038878166.18.4e-14691.553-hydroxybutyryl-CoA dehydrogenase [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q452231.0e-8255.523-hydroxybutyryl-CoA dehydrogenase OS=Bradyrhizobium diazoefficiens (strain JCM ... [more]
P458563.1e-7953.33Probable 3-hydroxybutyryl-CoA dehydrogenase OS=Bacillus subtilis (strain 168) OX... [more]
Q9RVG18.0e-7553.62Probable 3-hydroxybutyryl-CoA dehydrogenase OS=Deinococcus radiodurans (strain A... [more]
P520412.0e-7351.423-hydroxybutyryl-CoA dehydrogenase OS=Clostridium acetobutylicum (strain ATCC 82... [more]
P778517.8e-7048.753-hydroxybutyryl-CoA dehydrogenase OS=Thermoanaerobacterium thermosaccharolyticu... [more]
Match NameE-valueIdentityDescription
A0A0A0L6N51.9e-14892.23Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G234000 PE=3 SV=1[more]
A0A5A7USC99.6e-14892.573-hydroxybutyryl-CoA dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A1S3C2Y79.6e-14892.573-hydroxybutyryl-CoA dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103496074 PE=3 ... [more]
A0A5D3CH801.3e-14792.573-hydroxybutyryl-CoA dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1J8K21.3e-14489.53uncharacterized protein LOC111482724 OS=Cucurbita maxima OX=3661 GN=LOC111482724... [more]
Match NameE-valueIdentityDescription
AT3G15290.16.6e-12576.043-hydroxyacyl-CoA dehydrogenase family protein [more]
AT4G29010.11.3e-3532.20Enoyl-CoA hydratase/isomerase family [more]
AT3G06860.11.3e-3230.69multifunctional protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 1..189
e-value: 8.6E-68
score: 230.1
NoneNo IPR availablePIRSRPIRSR500134-3PIRSR500134-3coord: 7..91
e-value: 0.0033
score: 14.1
NoneNo IPR availablePANTHERPTHR48075:SF53-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEINcoord: 3..288
NoneNo IPR availablePANTHERPTHR480753-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEINcoord: 3..288
IPR0061763-hydroxyacyl-CoA dehydrogenase, NAD bindingPFAMPF027373HCDH_Ncoord: 7..184
e-value: 1.3E-64
score: 217.4
IPR0061083-hydroxyacyl-CoA dehydrogenase, C-terminalPFAMPF007253HCDHcoord: 187..283
e-value: 4.3E-35
score: 120.1
IPR0133286-phosphogluconate dehydrogenase, domain 2GENE3D1.10.1040.10coord: 190..295
e-value: 5.8E-33
score: 115.7
IPR0226943-hydroxyacyl-CoA dehydrogenasePIRSFPIRSF000105HCDHcoord: 1..295
e-value: 5.2E-92
score: 306.4
IPR0061803-hydroxyacyl-CoA dehydrogenase, conserved sitePROSITEPS000673HCDHcoord: 184..209
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 1..185
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 187..285

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0019538.1Lag0019538.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006631 fatty acid metabolic process
molecular_function GO:0004300 enoyl-CoA hydratase activity
molecular_function GO:0070403 NAD+ binding
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
molecular_function GO:0016491 oxidoreductase activity