Lag0019529 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0019529
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionglutamic acid-rich protein isoform X1
Locationchr5: 43065049 .. 43067399 (+)
RNA-Seq ExpressionLag0019529
SyntenyLag0019529
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCTGAGCGCCATTTTCGTACCACGAGCACCAACAGTACCACTTCAACCGCCACTGCCTCCAGCGAGCTTTTCATCTGCTTCACTTCTCGCTTCTCATCTTCTTCTTCTTCCTCCATGAAGATCTCTTCCAAGTCCATTCTCAGTCCCGGCCGAGCTCGCGAGCCCTCCCAAATCTCCCTCTCCACTTCCCTCAGCCGCCGTCTTAAATCCAGCGGCAGCCTCAAGGGCGGCCAGGCCTCCCCGATGTTTCCGACCGGCGGGAAGAAAAGAGGCTGCGCGTTTGAGAATCCGGAGCCGTCGTCTCCGAAGGTTACCTGCATTGGACAGGTTAGGGTTAAGACGAAGAAGCAAGGGAAGAAGATGAGGGCCAGATCGCTGAAGCGGAGGAGTAATTCGGAGGCGAGTTTTCGGAAATCGGAGAGTGTTCATTCGCAGGTGAATGGTAATGACCAGCAATTCGTGACGAAACAGTCGTCGCATCATAATCAGCATCTTCTTCGTCAGAATAGTAATAGTAATGGCGGAAATGGTTTCCAGCAGGAGTGCCTGTCGCATCGGAACCAGCGGTGGGTACATTTGCCGTTCACGATTTGCGAGGCGCTTAGGGCTTTTGGTGCTGAACTCAACTGCTTCTTGCCGTGCCATTCGTCGTGTTCGAGCGATAGGGAGAATCATAATAAGGAATCGAAGCCGGCGGAGAGGTCGTCGGAGAGTTCTTGCGGGACAGTGTTTGCGCGGTGGTTGGTGGCAGTGCAGGACGGCGACGGAAAAGGACGGGAGATCGAACTGGTGGTCGGAGACGAAGAAGCGCGAACGGAGAAGGAAAACGGAAGCCAGAGACGGCATGTTTTCGAGGGAATGGATTTCAAAGAGGAGAACGAAGTCGTGCAGGAAGAAGAATCGAGGATCAGCATTTGCATTCCGCCGAAGAATGCTTTGTTGCTAATGAGGTGCAGATCTGATCCAGTGAAAATGGCGGAGCTGGCGAAACGATTCTGTGAATCTCCTGCGCCAAAAGTGGACGAAGAAGACGAGGAAGAAGAAGAAGAGGATGAAGAGAAGAATAAACAAAATGAAGCGAAAAGAGATTTGCCCGTGCCCGTGCTTACGCCTTTGACTGTAACTGTAACTAAGGAAGAAGAAGAAGAAGAAAAGAGAGTAGAGCTAAATGTGAAGCTTAAAAACGAGGAAGAAATGAGTGAAGAAGAATTTGTATCTGATGCCGAAGTAGAAAACCAAGACGACGTTAATTTGGTTTTACAGGAAGAAGAAGAAGACGATGAAGAAGAAGCCATGGAAATGGCTACAGAAAACGAAATCGATGTGCAGAAATTAGACATTACAGTAGTAAATCAGAGACAAGAAGAACCAGCAGATGAAGAAAAAGAACAAGAAGAAGACGAGATCGATCAAGATAATCAGCAAGAGAAATCATCAGAAGAAACATTGCCGATTCCGATTACGATTCCGACCCACTGTGAACTCGAAATGGCTCGAGATGCAGAGACGCTGGAATCAATCGAAGAAGACGAATCCAAGCTACCCCATGGAAACGAACAAGACCAGAAAAGAGAAGAAGACGAAGACCAGAGAAGAGAACTTGAAGACGGCGAAAATGGCGAGAATCCCACATCGCCATCATTATCAGTAGAGACAGAACGAGTTTTAGAGGAAGCAGAAACTGAAGTTGATGGAAATTGGGAAGAAGAAGAAGAAGAAAGCAGAGGAAGGTCAACCGAAGAAGAAGTAAAAGAGACGACGACGAAAGCGACGGATGAAGGAATCGGACCCGACGCCCAAAACGACGACGAAATGGGTCCAGCGAAGGAGGACCAGTCAAAGGAGCGAGAAACTCCGCCGCCGGAGCCGGAGAGAGAAACACAAACCCAAACAGAAACAAAACCAGAGACCTCCGTCCTCCCAGATTGCTTGCTGTTGATGATGTACGAGCCGAAGCTATCAATGGAGGTATCAAAGGAGACATGGGTTTGCAGCACAGATTTCATAAGGTGCGTTCCGACGAGGGAGAAGAAGGCGGCTCCGCCACCGCCGCCGAAGAAGCGGGAGACGAAGCCGGCGGAGAACAACACGCAGGCGACGGCGGCGGTGCAGCCGGGGAGATGGTCGTGTTCGTTTCCGGCGGCGGCAGCGGCGGCGGCGATGATAGAGCAGAAGCTAGTGAGGGCGAAGGGGGGTACGAGCCGTTTGTTCTGACGAGGTGCAAGTCGGAGCCGATGAGGTCATCGGCTAAGCTGGCGCCAGACGCTTGCTTTTGGAAGGATAGGAAGCTTGAGCCGCACCGTCCGGCTACGTTCGGCGTCGGCGCGGCTGGAGTTGGATTTTGA

mRNA sequence

ATGGATTCTGAGCGCCATTTTCGTACCACGAGCACCAACAGTACCACTTCAACCGCCACTGCCTCCAGCGAGCTTTTCATCTGCTTCACTTCTCGCTTCTCATCTTCTTCTTCTTCCTCCATGAAGATCTCTTCCAAGTCCATTCTCAGTCCCGGCCGAGCTCGCGAGCCCTCCCAAATCTCCCTCTCCACTTCCCTCAGCCGCCGTCTTAAATCCAGCGGCAGCCTCAAGGGCGGCCAGGCCTCCCCGATGTTTCCGACCGGCGGGAAGAAAAGAGGCTGCGCGTTTGAGAATCCGGAGCCGTCGTCTCCGAAGGTTACCTGCATTGGACAGGTTAGGGTTAAGACGAAGAAGCAAGGGAAGAAGATGAGGGCCAGATCGCTGAAGCGGAGGAGTAATTCGGAGGCGAGTTTTCGGAAATCGGAGAGTGTTCATTCGCAGGTGAATGGTAATGACCAGCAATTCGTGACGAAACAGTCGTCGCATCATAATCAGCATCTTCTTCGTCAGAATAGTAATAGTAATGGCGGAAATGGTTTCCAGCAGGAGTGCCTGTCGCATCGGAACCAGCGGTGGGTACATTTGCCGTTCACGATTTGCGAGGCGCTTAGGGCTTTTGGTGCTGAACTCAACTGCTTCTTGCCGTGCCATTCGTCGTGTTCGAGCGATAGGGAGAATCATAATAAGGAATCGAAGCCGGCGGAGAGGTCGTCGGAGAGTTCTTGCGGGACAGTGTTTGCGCGGTGGTTGGTGGCAGTGCAGGACGGCGACGGAAAAGGACGGGAGATCGAACTGGTGGTCGGAGACGAAGAAGCGCGAACGGAGAAGGAAAACGGAAGCCAGAGACGGCATGTTTTCGAGGGAATGGATTTCAAAGAGGAGAACGAAGTCGTGCAGGAAGAAGAATCGAGGATCAGCATTTGCATTCCGCCGAAGAATGCTTTGTTGCTAATGAGGTGCAGATCTGATCCAGTGAAAATGGCGGAGCTGGCGAAACGATTCTGTGAATCTCCTGCGCCAAAAGTGGACGAAGAAGACGAGGAAGAAGAAGAAGAGGATGAAGAGAAGAATAAACAAAATGAAGCGAAAAGAGATTTGCCCGTGCCCGTGCTTACGCCTTTGACTGTAACTGTAACTAAGGAAGAAGAAGAAGAAGAAAAGAGAGTAGAGCTAAATGTGAAGCTTAAAAACGAGGAAGAAATGAGTGAAGAAGAATTTGTATCTGATGCCGAAGTAGAAAACCAAGACGACGTTAATTTGGTTTTACAGGAAGAAGAAGAAGACGATGAAGAAGAAGCCATGGAAATGGCTACAGAAAACGAAATCGATGTGCAGAAATTAGACATTACAGTAGTAAATCAGAGACAAGAAGAACCAGCAGATGAAGAAAAAGAACAAGAAGAAGACGAGATCGATCAAGATAATCAGCAAGAGAAATCATCAGAAGAAACATTGCCGATTCCGATTACGATTCCGACCCACTGTGAACTCGAAATGGCTCGAGATGCAGAGACGCTGGAATCAATCGAAGAAGACGAATCCAAGCTACCCCATGGAAACGAACAAGACCAGAAAAGAGAAGAAGACGAAGACCAGAGAAGAGAACTTGAAGACGGCGAAAATGGCGAGAATCCCACATCGCCATCATTATCAGTAGAGACAGAACGAGTTTTAGAGGAAGCAGAAACTGAAGTTGATGGAAATTGGGAAGAAGAAGAAGAAGAAAGCAGAGGAAGGTCAACCGAAGAAGAAGTAAAAGAGACGACGACGAAAGCGACGGATGAAGGAATCGGACCCGACGCCCAAAACGACGACGAAATGGGTCCAGCGAAGGAGGACCAGTCAAAGGAGCGAGAAACTCCGCCGCCGGAGCCGGAGAGAGAAACACAAACCCAAACAGAAACAAAACCAGAGACCTCCGTCCTCCCAGATTGCTTGCTGTTGATGATATTTCATAAGGTGCGTTCCGACGAGGGAGAAGAAGGCGGCTCCGCCACCGCCGCCGAAGAAGCGGGAGACGAAGCCGGCGGAGAACAACACGCAGGCGACGGCGGCGGTGCAGCCGGGGAGATGGTCGTGTTCGTTTCCGGCGGCGGCAGCGGCGGCGGCGATGATAGAGCAGAAGCTAGTGAGGGCGAAGGGGGGTACGAGCCGTTTGTTCTGACGAGGTGCAAGTCGGAGCCGATGAGGTCATCGGCTAAGCTGGCGCCAGACGCTTGCTTTTGGAAGGATAGGAAGCTTGAGCCGCACCGTCCGGCTACGTTCGGCGTCGGCGCGGCTGGAGTTGGATTTTGA

Coding sequence (CDS)

ATGGATTCTGAGCGCCATTTTCGTACCACGAGCACCAACAGTACCACTTCAACCGCCACTGCCTCCAGCGAGCTTTTCATCTGCTTCACTTCTCGCTTCTCATCTTCTTCTTCTTCCTCCATGAAGATCTCTTCCAAGTCCATTCTCAGTCCCGGCCGAGCTCGCGAGCCCTCCCAAATCTCCCTCTCCACTTCCCTCAGCCGCCGTCTTAAATCCAGCGGCAGCCTCAAGGGCGGCCAGGCCTCCCCGATGTTTCCGACCGGCGGGAAGAAAAGAGGCTGCGCGTTTGAGAATCCGGAGCCGTCGTCTCCGAAGGTTACCTGCATTGGACAGGTTAGGGTTAAGACGAAGAAGCAAGGGAAGAAGATGAGGGCCAGATCGCTGAAGCGGAGGAGTAATTCGGAGGCGAGTTTTCGGAAATCGGAGAGTGTTCATTCGCAGGTGAATGGTAATGACCAGCAATTCGTGACGAAACAGTCGTCGCATCATAATCAGCATCTTCTTCGTCAGAATAGTAATAGTAATGGCGGAAATGGTTTCCAGCAGGAGTGCCTGTCGCATCGGAACCAGCGGTGGGTACATTTGCCGTTCACGATTTGCGAGGCGCTTAGGGCTTTTGGTGCTGAACTCAACTGCTTCTTGCCGTGCCATTCGTCGTGTTCGAGCGATAGGGAGAATCATAATAAGGAATCGAAGCCGGCGGAGAGGTCGTCGGAGAGTTCTTGCGGGACAGTGTTTGCGCGGTGGTTGGTGGCAGTGCAGGACGGCGACGGAAAAGGACGGGAGATCGAACTGGTGGTCGGAGACGAAGAAGCGCGAACGGAGAAGGAAAACGGAAGCCAGAGACGGCATGTTTTCGAGGGAATGGATTTCAAAGAGGAGAACGAAGTCGTGCAGGAAGAAGAATCGAGGATCAGCATTTGCATTCCGCCGAAGAATGCTTTGTTGCTAATGAGGTGCAGATCTGATCCAGTGAAAATGGCGGAGCTGGCGAAACGATTCTGTGAATCTCCTGCGCCAAAAGTGGACGAAGAAGACGAGGAAGAAGAAGAAGAGGATGAAGAGAAGAATAAACAAAATGAAGCGAAAAGAGATTTGCCCGTGCCCGTGCTTACGCCTTTGACTGTAACTGTAACTAAGGAAGAAGAAGAAGAAGAAAAGAGAGTAGAGCTAAATGTGAAGCTTAAAAACGAGGAAGAAATGAGTGAAGAAGAATTTGTATCTGATGCCGAAGTAGAAAACCAAGACGACGTTAATTTGGTTTTACAGGAAGAAGAAGAAGACGATGAAGAAGAAGCCATGGAAATGGCTACAGAAAACGAAATCGATGTGCAGAAATTAGACATTACAGTAGTAAATCAGAGACAAGAAGAACCAGCAGATGAAGAAAAAGAACAAGAAGAAGACGAGATCGATCAAGATAATCAGCAAGAGAAATCATCAGAAGAAACATTGCCGATTCCGATTACGATTCCGACCCACTGTGAACTCGAAATGGCTCGAGATGCAGAGACGCTGGAATCAATCGAAGAAGACGAATCCAAGCTACCCCATGGAAACGAACAAGACCAGAAAAGAGAAGAAGACGAAGACCAGAGAAGAGAACTTGAAGACGGCGAAAATGGCGAGAATCCCACATCGCCATCATTATCAGTAGAGACAGAACGAGTTTTAGAGGAAGCAGAAACTGAAGTTGATGGAAATTGGGAAGAAGAAGAAGAAGAAAGCAGAGGAAGGTCAACCGAAGAAGAAGTAAAAGAGACGACGACGAAAGCGACGGATGAAGGAATCGGACCCGACGCCCAAAACGACGACGAAATGGGTCCAGCGAAGGAGGACCAGTCAAAGGAGCGAGAAACTCCGCCGCCGGAGCCGGAGAGAGAAACACAAACCCAAACAGAAACAAAACCAGAGACCTCCGTCCTCCCAGATTGCTTGCTGTTGATGATATTTCATAAGGTGCGTTCCGACGAGGGAGAAGAAGGCGGCTCCGCCACCGCCGCCGAAGAAGCGGGAGACGAAGCCGGCGGAGAACAACACGCAGGCGACGGCGGCGGTGCAGCCGGGGAGATGGTCGTGTTCGTTTCCGGCGGCGGCAGCGGCGGCGGCGATGATAGAGCAGAAGCTAGTGAGGGCGAAGGGGGGTACGAGCCGTTTGTTCTGACGAGGTGCAAGTCGGAGCCGATGAGGTCATCGGCTAAGCTGGCGCCAGACGCTTGCTTTTGGAAGGATAGGAAGCTTGAGCCGCACCGTCCGGCTACGTTCGGCGTCGGCGCGGCTGGAGTTGGATTTTGA

Protein sequence

MDSERHFRTTSTNSTTSTATASSELFICFTSRFSSSSSSSMKISSKSILSPGRAREPSQISLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKKQGKKMRARSLKRRSNSEASFRKSESVHSQVNGNDQQFVTKQSSHHNQHLLRQNSNSNGGNGFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSDRENHNKESKPAERSSESSCGTVFARWLVAVQDGDGKGREIELVVGDEEARTEKENGSQRRHVFEGMDFKEENEVVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESPAPKVDEEDEEEEEEDEEKNKQNEAKRDLPVPVLTPLTVTVTKEEEEEEKRVELNVKLKNEEEMSEEEFVSDAEVENQDDVNLVLQEEEEDDEEEAMEMATENEIDVQKLDITVVNQRQEEPADEEKEQEEDEIDQDNQQEKSSEETLPIPITIPTHCELEMARDAETLESIEEDESKLPHGNEQDQKREEDEDQRRELEDGENGENPTSPSLSVETERVLEEAETEVDGNWEEEEEESRGRSTEEEVKETTTKATDEGIGPDAQNDDEMGPAKEDQSKERETPPPEPERETQTQTETKPETSVLPDCLLLMIFHKVRSDEGEEGGSATAAEEAGDEAGGEQHAGDGGGAAGEMVVFVSGGGSGGGDDRAEASEGEGGYEPFVLTRCKSEPMRSSAKLAPDACFWKDRKLEPHRPATFGVGAAGVGF
Homology
BLAST of Lag0019529 vs. NCBI nr
Match: XP_038894264.1 (glutamic acid-rich protein [Benincasa hispida])

HSP 1 Score: 916.8 bits (2368), Expect = 1.3e-262
Identity = 579/797 (72.65%), Postives = 630/797 (79.05%), Query Frame = 0

Query: 1   MDSERHFRTTSTNSTTSTATASSELFICFTSRF-SSSSSSSMKISSKSILSPGRAREPSQ 60
           MD++RHFRTTSTNST+STA  SSELFICFTSRF SSSSSSSMKISSKSILSPGRAREPSQ
Sbjct: 1   MDADRHFRTTSTNSTSSTAAPSSELFICFTSRFSSSSSSSSMKISSKSILSPGRAREPSQ 60

Query: 61  ISLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKKQ 120
           ISLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAF+NPEPSSPKVTCIGQVRVKTKKQ
Sbjct: 61  ISLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFDNPEPSSPKVTCIGQVRVKTKKQ 120

Query: 121 GKKMRARSLKRRSNSEASFRKSESV--HSQVNGNDQQFVTKQSSHHNQHLLRQNSNSNGG 180
           GKKMRARS KRRSNSEASFR+SESV   SQ+NGN+QQF    SSHHN HLLRQNSN+NGG
Sbjct: 121 GKKMRARSHKRRSNSEASFRRSESVVQSSQMNGNEQQF----SSHHNHHLLRQNSNNNGG 180

Query: 181 NGFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSDRENHNKESKPAERS 240
           NGFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSDREN NKESKPA RS
Sbjct: 181 NGFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSDREN-NKESKPAVRS 240

Query: 241 S--ESSCGTVFARWLVAVQDGDGKGREIELVVGDEEARTEKENGSQRRHVFEGMDFKEEN 300
           S  ESSCGTVFARWLVAVQDGDGKGREIELVVGDEE RTEKENGSQRRHVFEG+DFK+EN
Sbjct: 241 SETESSCGTVFARWLVAVQDGDGKGREIELVVGDEETRTEKENGSQRRHVFEGLDFKDEN 300

Query: 301 EVVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESPAPKVDEED--EEEEEED 360
           E+V++EESRISICIPPKNALLLMRCRSDPVKMAELAKRFC+SPAPKVDEED  EEEEE++
Sbjct: 301 EIVEQEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCDSPAPKVDEEDGEEEEEEDN 360

Query: 361 EEKNKQNEAKRDLPVPVLTPL--TVTVTKEEEEEEKRVELNVKLKNEEEMSEEEFVSDAE 420
           E KNKQNE KRD+ VP+  P+  TVTV+KE+EEEE++VEL VKL+N+EE +EE  V D+E
Sbjct: 361 EAKNKQNEVKRDVSVPLPVPVSSTVTVSKEKEEEERKVELIVKLENDEETNEES-VFDSE 420

Query: 421 VENQDDVNLVLQ---EEEEDDEEEAMEMATENEIDVQKLDITVVNQRQEEPADEEKEQEE 480
            EN   VNL LQ   EEEED+ E  +EMAT NEID QKLDI V+NQ  +E A EEKE EE
Sbjct: 421 KEN-GQVNLFLQEEREEEEDNRERTIEMATVNEIDEQKLDIIVINQPNQEQAVEEKE-EE 480

Query: 481 DEIDQDNQQEKSSEETLPIPITIPTHCELEMARDAETLESIEEDESKLPHGNEQDQKREE 540
           D+IDQDNQQE  +   +PIPI I TH E E A+DAE LES+EEDESKLPH +EQDQK E 
Sbjct: 481 DKIDQDNQQETMA---IPIPIPIETHYEPETAQDAEKLESVEEDESKLPHESEQDQKTE- 540

Query: 541 DEDQRRELEDGENGENPTSPSLSVETERVLEEAETEVDGNWEEEEEESRGRSTEEEVKET 600
            ++  RE E+  NGENPTSPS SVETE VL+E ETEVD NWEEEEEE      EEE +E 
Sbjct: 541 -DENLREEEEPVNGENPTSPSFSVETEPVLDETETEVDWNWEEEEEEEEEEEEEEEEEE- 600

Query: 601 TTKATDEGIGPDAQNDDEMGPAKEDQSKERETPPPEPERETQTQTETKPETSVLPDCLLL 660
             KATDEGIGPDAQNDD++   +EDQSKERETP PEPER+TQT      ETSVLPDCLLL
Sbjct: 601 --KATDEGIGPDAQNDDKLMGPEEDQSKERETPRPEPERKTQT------ETSVLPDCLLL 660

Query: 661 MIFHKVRSDE------------------GEEGGSATAAEEAGDEAGGEQHAGDGGGAAGE 720
           M++    S E                   E+  +             E    D   AA  
Sbjct: 661 MMYEPKLSMEVSKETWVCSTDFIRCVPTREKKPACRNPPPPPPPKKRETKPADTTQAAVV 720

Query: 721 MVVFVSGGGSGGGDDRAEASE---GEGGYEPFVLTRCKSEPMRSSAKLAPDACFWKDRKL 765
                S          A   +      GYEPFVLTRCKSEPMRSSAKLAPDACFWKDRKL
Sbjct: 721 QPARWSCSFPAAAAAAAMIEQKLVRAKGYEPFVLTRCKSEPMRSSAKLAPDACFWKDRKL 775

BLAST of Lag0019529 vs. NCBI nr
Match: XP_022941617.1 (glutamic acid-rich protein-like [Cucurbita moschata])

HSP 1 Score: 904.8 bits (2337), Expect = 5.0e-259
Identity = 570/796 (71.61%), Postives = 628/796 (78.89%), Query Frame = 0

Query: 1   MDSERHFRTTSTNSTTSTATASSELFICFTSRFSSSSSSSMKISSKSILSPGRAREPSQI 60
           MDS+RHFR  +T S+T+TA+ASSELFICFTSRFSSSSSSSMKISSKSILSPGR REP+QI
Sbjct: 1   MDSDRHFR--NTTSSTATASASSELFICFTSRFSSSSSSSMKISSKSILSPGRPREPAQI 60

Query: 61  SLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKKQG 120
           SLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKKQG
Sbjct: 61  SLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKKQG 120

Query: 121 KKMRARSLKRRSNSEASFRKSES--VHSQVNGNDQQFVTKQSSHHNQHLLRQNSNSNGGN 180
           KKMRARSLKRRSNSEASFRKSES  V SQ+NGND      QSSH N HLLRQ  NSNGGN
Sbjct: 121 KKMRARSLKRRSNSEASFRKSESVQVQSQMNGND---FVNQSSHLNHHLLRQ--NSNGGN 180

Query: 181 GFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSDRENHNKESKPAERS- 240
           GFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSS+REN NKESK  ERS 
Sbjct: 181 GFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSNREN-NKESKTTERSS 240

Query: 241 -SESSCGTVFARWLVAVQDGDGKGREIELVVGDEEARTEKENGSQRRHVFEGMDFKEENE 300
            SESSCGTVFARWLVAVQD DG+GREIELVVGDEE+RTEKENGSQRRHVFEG+DFKEE E
Sbjct: 241 ESESSCGTVFARWLVAVQDNDGRGREIELVVGDEESRTEKENGSQRRHVFEGLDFKEEKE 300

Query: 301 VVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESPAPKVDEEDEEEEEEDEEK 360
           VVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESP PK++EED  EEE DEE 
Sbjct: 301 VVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESPVPKLEEED--EEENDEEN 360

Query: 361 NKQNEAKRDLPVPVLTPLTVTVTKEEEEEEKRVELNVKLKNEEEMSEEEFVSDAEVENQD 420
           + QNEA++  P+PVL PLTVT+ KEEEEEE +VELN KLKNEEEM EE  VSDAE E ++
Sbjct: 361 SNQNEAQKGTPLPVLMPLTVTLIKEEEEEETKVELNSKLKNEEEMIEES-VSDAE-EEEE 420

Query: 421 DVNLVLQEEEEDDE---EEAMEMATENEIDVQKLDITVVNQRQEEPADEEKEQE---EDE 480
           + N+VLQEEEE++E   EE++EMATENEIDVQKLDITV+N + +E A+E+KEQE   E  
Sbjct: 421 EANVVLQEEEEEEEDNGEESIEMATENEIDVQKLDITVINHQDQEEAEEDKEQEHEQEHR 480

Query: 481 IDQDNQQEKSSEETLPIPITIPTHCELEMARDAETLESIEEDESKLPHGNEQDQKREEDE 540
           IDQDNQQ+K  EET+   I I T CE EM +DAE LES E DE K  HGNEQD + EE  
Sbjct: 481 IDQDNQQQKLVEETMAFSIPISTQCEPEMVQDAEKLESAEGDEFKPFHGNEQDFETEEQM 540

Query: 541 DQRRELEDGENGENPTSPSLSVETERVLEEAETEVDGNWEEEEEESRGRSTEEEVKETTT 600
            +  E E  ENGENPTSP LSV         ETEVDGNW EEEEE+RGRSTEEE+K T  
Sbjct: 541 KELEEEEKSENGENPTSPPLSV---------ETEVDGNW-EEEEENRGRSTEEELKGTAA 600

Query: 601 KATDEGIGPDAQNDDEMG-PAKEDQSKERETPPPEPERETQTQTETKPETSVLPDCLLLM 660
            A DEGIGP  QNDDEMG   +EDQSKERETPPPEPERETQTQ  TKPE SVLPDCLLLM
Sbjct: 601 TAMDEGIGPHIQNDDEMGLEEEEDQSKERETPPPEPERETQTQ--TKPEASVLPDCLLLM 660

Query: 661 IFH-KVRSDEGEEGGSA---------TAAEEAGDEAGGEQHAGDGGGAAGEMVVFVSGG- 720
           ++  K+  +  +E             T  ++A      ++          +  V +  G 
Sbjct: 661 MYEPKLSMEVSKETWVCSTDFIRCVPTREKKAAPPPPPKKREIKTAEKNTQTQVAIQPGR 720

Query: 721 ----------GSGGGDDRAEASEGEGGYEPFVLTRCKSEPMRSSAKLAPDACFWKDRKLE 765
                      +   + + E ++G GGYEPFVLTRCKSEPMRSSAKLAPD  F KDR LE
Sbjct: 721 WSCSFPAAAAAAAMIEQKLERAKG-GGYEPFVLTRCKSEPMRSSAKLAPDTGFCKDRNLE 771

BLAST of Lag0019529 vs. NCBI nr
Match: KAG7031049.1 (hypothetical protein SDJN02_05088, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 902.1 bits (2330), Expect = 3.2e-258
Identity = 577/811 (71.15%), Postives = 635/811 (78.30%), Query Frame = 0

Query: 1   MDSERHFRTTSTNSTTSTATASSELFICFTSRFSSSSSSSMKISSKSILSPGRAREPSQI 60
           MDS+RHFR  +T S+T+TA+ASSELFICFTSRFSSSSSSSMKISSKSILSPGR REP+QI
Sbjct: 1   MDSDRHFR--NTTSSTATASASSELFICFTSRFSSSSSSSMKISSKSILSPGRPREPAQI 60

Query: 61  SLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKKQG 120
           SLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKKQG
Sbjct: 61  SLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKKQG 120

Query: 121 KKMRARSLKRRSNSEASFRKSES--VHSQVNGNDQQFVTKQSSHHNQHLLRQNSNSNGGN 180
           KKMRARSLKRRSNSEASFRKSES  V SQ+NGND      QSSH N HLLRQ  NSNGGN
Sbjct: 121 KKMRARSLKRRSNSEASFRKSESVQVQSQMNGND---FVNQSSHLNHHLLRQ--NSNGGN 180

Query: 181 GFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSDRENHNKESKPAERS- 240
           GFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSS+REN NKESK  ERS 
Sbjct: 181 GFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSNREN-NKESKTTERSS 240

Query: 241 -SESSCGTVFARWLVAVQDGDGKGREIELVVGDEEARTEKENGSQRRHVFEGMDFKEENE 300
            SESSCGTVFARWLVAVQD DG+GREIELVVGDEE+RTEKENGSQRRHVFEG+DFKEE E
Sbjct: 241 ESESSCGTVFARWLVAVQDNDGRGREIELVVGDEESRTEKENGSQRRHVFEGLDFKEEKE 300

Query: 301 VVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESPAPKVDEEDEEEEEEDEEK 360
           VVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESP PK++EED  EEE DEE 
Sbjct: 301 VVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESPVPKLEEED--EEENDEEN 360

Query: 361 NKQNEAKRDLPVPVLTPLTVTVTKEEEEEEK--RVELNVKLKNEEEMSEEEFVSDA---- 420
           + QNEAK+  P+PVL PLTVT+ KEEEEEE+  +VELN KLKNEEEM EE  VSDA    
Sbjct: 361 SNQNEAKKGTPLPVLMPLTVTLIKEEEEEEEETKVELNSKLKNEEEMIEES-VSDAEEEE 420

Query: 421 -EVENQDDVNLVLQEEEEDDE---EEAMEMATENEIDVQKLDITVVN-QRQEEPADEEKE 480
            E E +++ N+VLQEEEE++E   EE++EMATENEIDVQKLDITV+N Q QEE A+E+KE
Sbjct: 421 EEEEEEEEANVVLQEEEEEEEDSGEESIEMATENEIDVQKLDITVINHQDQEEEAEEDKE 480

Query: 481 QE-----EDEIDQDNQQEKSSEETLPIPITIPTHCELEMARDAETLESIEEDESKLPHGN 540
           QE     E  IDQDNQQ+K  EET+   I I T CE EM +DAE LES E DE K  HGN
Sbjct: 481 QEHEQEQEHRIDQDNQQQKLVEETMAFSIPISTQCEPEMVQDAEKLESAEGDEFKPFHGN 540

Query: 541 EQDQKREEDEDQRRELED---GENGENPTSPSLSVETERVLEEAETEVDGNW--EEEEEE 600
           EQD + EE E+Q +E E+    ENGENPTSP LSV         ETEVDGNW  EEEEEE
Sbjct: 541 EQDFETEEHEEQMKEFEEEEKSENGENPTSPPLSV---------ETEVDGNWEEEEEEEE 600

Query: 601 SRGRSTEEEVKETTTKATDEGIGPDAQNDDEMG-PAKEDQSKERETPPPEPERETQTQTE 660
           +RGRSTEEE+K T   A DEGIGP  QNDDEMG   +EDQSKERETPPPEPERETQTQ  
Sbjct: 601 NRGRSTEEELKGTAATAMDEGIGPHIQNDDEMGLEEEEDQSKERETPPPEPERETQTQ-- 660

Query: 661 TKPETSVLPDCLLLMIFH-KVRSDEGEEGGSA---------TAAEEAGDEAGGEQHAGDG 720
           TKPE SVLPDCLLLM++  K+  +  +E             T  ++A      ++     
Sbjct: 661 TKPEASVLPDCLLLMMYEPKLSMEVSKETWVCSTDFIRCVPTREKKAAPPPPPKKREIKT 720

Query: 721 GGAAGEMVVFVSGG-----------GSGGGDDRAEASEGEGGYEPFVLTRCKSEPMRSSA 765
                +  V +  G            +   + + E ++G GGYEPFVLTRCKSEPMRSSA
Sbjct: 721 AEKNTQTQVAIQPGRWSCSFPAAAAAAAMIEQKLERAKG-GGYEPFVLTRCKSEPMRSSA 780

BLAST of Lag0019529 vs. NCBI nr
Match: XP_023535806.1 (glutamic acid-rich protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 887.9 bits (2293), Expect = 6.3e-254
Identity = 572/808 (70.79%), Postives = 632/808 (78.22%), Query Frame = 0

Query: 1   MDSERHFRTTSTNSTTSTATASSELFICFTSRFSSSSSSSMKISSKSILSPGRAREPSQI 60
           MDS+R FR  +T S+T+TA+ASSELFICFTSRFSSSSSSSMKISSKSILSPGR REP+QI
Sbjct: 1   MDSDRQFR--NTTSSTATASASSELFICFTSRFSSSSSSSMKISSKSILSPGRPREPAQI 60

Query: 61  SLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKKQG 120
           SLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKKQG
Sbjct: 61  SLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKKQG 120

Query: 121 KKMRARSLKRRSNSEASFRKSES--VHSQVNGNDQQFVTKQSSHHNQHLLRQNSNSNGGN 180
           KKMRARSLKRRSNSEASFRKSES  V SQ+NGND      QSSHHN HLLRQ  NSNGGN
Sbjct: 121 KKMRARSLKRRSNSEASFRKSESVQVQSQMNGND---FVNQSSHHN-HLLRQ--NSNGGN 180

Query: 181 GFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSDRENHNKESKPAERS- 240
            FQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSS+REN NKESK  ERS 
Sbjct: 181 SFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSNREN-NKESKTTERSS 240

Query: 241 -SESSCGTVFARWLVAVQDGDGKGREIELVVGDEEARTEKENGSQRRHVFEGMDFKEENE 300
            SESSCGTVFARWLVAVQD DG+GREIELVVGDEE+RTEKENGSQRRHVFEG+DFKEE E
Sbjct: 241 ESESSCGTVFARWLVAVQDNDGRGREIELVVGDEESRTEKENGSQRRHVFEGLDFKEEKE 300

Query: 301 VVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESPAPKVDEEDEEEEEEDEEK 360
           VVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESP PK++EED  EEE DEE 
Sbjct: 301 VVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESPVPKLEEED--EEENDEEN 360

Query: 361 NKQNEAKRDLPVPVLTPLTVTVTKEEEEEEK--RVELNVKLKNEEEMSEEEFVSDAEVEN 420
           + QNEAK+  P+PVL PLTVT+ KEEEEEE+  +VELN KLKNEEEM EE  VSDAE E 
Sbjct: 361 SNQNEAKKGTPLPVLMPLTVTLIKEEEEEEEETKVELNSKLKNEEEMIEES-VSDAEEEE 420

Query: 421 QDDVNLVLQEEEEDDE---EEAMEMATENEIDVQKLDITVVN-QRQEEPADEEKEQE--- 480
           +++ N+VLQEEEE++E   EE++EMATENEIDVQKLDITV+N Q QEE A+E+KEQE   
Sbjct: 421 EEEANVVLQEEEEEEEDNGEESIEMATENEIDVQKLDITVINHQDQEEEAEEDKEQEHEQ 480

Query: 481 --EDEIDQDNQQEKSSEETLPIPITIPTHCELEMARDAETLESIEEDESKLPHGNEQD-- 540
             E  IDQDNQQ+K  EET+   I I T CE EM +DAE LES E DE K  HGNEQD  
Sbjct: 481 EQEHRIDQDNQQQKLVEETMAFSIPISTQCEPEMVQDAEKLESAEGDEFKPFHGNEQDFE 540

Query: 541 -QKREEDEDQRRELED---GENGENPTSPSLSVETERVLEEAETEVDGNW-EEEEEESRG 600
            ++ EE E+Q ++LE+    ENGENPTS  LSV         ET VDGNW EEEEEE+RG
Sbjct: 541 TEEHEEHEEQMKDLEEEEKSENGENPTSRPLSV---------ETAVDGNWEEEEEEENRG 600

Query: 601 RSTEEEVKETTTKATDEGIGPDAQNDDEMG-PAKEDQSKERETPPPEPERETQTQTETKP 660
           RSTEEE+K T   A DEGIGP  QNDDEMG   +EDQSKERETPPPE ERETQTQ  TKP
Sbjct: 601 RSTEEELKGTAATAMDEGIGPHIQNDDEMGLEEEEDQSKERETPPPELERETQTQ--TKP 660

Query: 661 ETSVLPDCLLLMIFH-KVRSDEGEEGGSA---------TAAEEAGDEAGGEQHAGDGGGA 720
           E SVLPDCLLLM++  K+  +  +E             T  ++A      ++        
Sbjct: 661 EASVLPDCLLLMMYEPKLSMEVSKETWVCSTDFIRCVPTREKKAAPPPPPKKREIKTAEK 720

Query: 721 AGEMVVFVSGG-----------GSGGGDDRAEASEGEGGYEPFVLTRCKSEPMRSSAKLA 765
             +  V +  G            +   + + E ++G GGYEPFVLTRCKSEPMRSSAKLA
Sbjct: 721 NTQTQVAIQPGRWSCSFPAAAAAAAMIEQKLERAKG-GGYEPFVLTRCKSEPMRSSAKLA 780

BLAST of Lag0019529 vs. NCBI nr
Match: XP_022979182.1 (calponin homology domain-containing protein DDB_G0272472-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 884.8 bits (2285), Expect = 5.3e-253
Identity = 570/795 (71.70%), Postives = 620/795 (77.99%), Query Frame = 0

Query: 1   MDSERHFRTTSTNSTTSTATASSELFICFTSRF--SSSSSSSMKISSKSILSPGRAREPS 60
           MDS+RHFR  +T S+T++A+ASSELFICFTSR   SSSSSSSMKISSKSILSPGR REP+
Sbjct: 1   MDSDRHFR--NTTSSTASASASSELFICFTSRISSSSSSSSSMKISSKSILSPGRPREPA 60

Query: 61  QISLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKK 120
           QISLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGC FENPEPSSPKVTCIGQVRVKTKK
Sbjct: 61  QISLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCGFENPEPSSPKVTCIGQVRVKTKK 120

Query: 121 QGKKMRARSLKRRSNSEASFRKSES--VHSQVNGNDQQFVTKQSSHHNQHLLRQNSNSNG 180
           QGKKMRARSLKRRSNSEASFRKSES  V SQ+NGND      QSSHHN HLLRQ  NSNG
Sbjct: 121 QGKKMRARSLKRRSNSEASFRKSESVQVQSQMNGND---FMNQSSHHNHHLLRQ--NSNG 180

Query: 181 GNGFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSDRENHNKESKPAER 240
           GNGFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSS+REN NKESK  ER
Sbjct: 181 GNGFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSNREN-NKESKTTER 240

Query: 241 S--SESSCGTVFARWLVAVQDGDGKGREIELVVGDEEARTEKENGSQRRHVFEGMDFKEE 300
           S  SESSCGTVFARWLVAVQD DG+GREIELVVGDEE+RTEK+NGSQRRHVFEG+DFKEE
Sbjct: 241 SSESESSCGTVFARWLVAVQDNDGRGREIELVVGDEESRTEKDNGSQRRHVFEGLDFKEE 300

Query: 301 NEVVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESPAPKVDEEDEEEEEEDE 360
            EVVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESP PK+   +EEEEE+DE
Sbjct: 301 KEVVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESPVPKL---EEEEEEDDE 360

Query: 361 EKNKQNEAKRDLPVPVLTPLTVTVTKEEEEEEKRVELNVKLKNEEEMSEEEFVSDAEVEN 420
           E + QNEAK+  P+PVL PL+VT+ KEEEEE K VELN KLKNEEEM EE  VSDAE E 
Sbjct: 361 ENSNQNEAKKGAPLPVLVPLSVTLIKEEEEETK-VELNSKLKNEEEMIEES-VSDAEEE- 420

Query: 421 QDDVNLVLQEEEEDD--EEEAMEMATENEIDVQKLDITVVN-QRQEEPADEEKEQEEDEI 480
            ++ NLVLQEE+E++   EE++EMATENEIDVQKLDITV N Q QEE A+E+KEQE   I
Sbjct: 421 -EEANLVLQEEKEEEVNGEESIEMATENEIDVQKLDITVTNHQDQEEEAEEDKEQEH-RI 480

Query: 481 DQDNQQEKSSEETLPIPITIPTHCELEMARDAETLESIEEDESKLPHGNEQDQKREEDED 540
           DQDNQQ+K  EET+   I I T CE EM +DAE LES E DE K  HGNEQD + EE   
Sbjct: 481 DQDNQQQK-LEETMAFSIPISTRCEPEMVQDAEKLESAEGDEFKPSHGNEQDFETEEQMK 540

Query: 541 QRRELEDGENGENPTSPSLSVETERVLEEAETEVDGNW---EEEEEESRGRSTEEEVKET 600
           +  E E  ENGENPTSP LSV         ET VDGNW   EEEEEE+RGRST EE+K T
Sbjct: 541 ELEEEEKSENGENPTSPPLSV---------ETAVDGNWEEEEEEEEENRGRST-EELKGT 600

Query: 601 TTKATDEGIGPDAQNDDEMGPAKEDQSKERETPPPEPERETQTQTETKPETSVLPDCLLL 660
           T  A DEGIGP  QNDDEMG  +EDQSKERETPPPEPERETQTQ  TKPE SVLPDCLLL
Sbjct: 601 TATAMDEGIGPHIQNDDEMGLEEEDQSKERETPPPEPERETQTQ--TKPEASVLPDCLLL 660

Query: 661 MIFH-KVRSDEGEEGGSATA----------AEEAGDEAGGEQHAGDGGGAAGEMVVFVSG 720
           M++  K+  +  +E    +            + A   A  ++     G      V    G
Sbjct: 661 MMYEPKLSMEVSKETWVCSTDFIRCVPTREKKAAPPPAPKKREIKTAGKHTQTQVAIQPG 720

Query: 721 GGSGGGDDRAEAS--------EGEGGYEPFVLTRCKSEPMRSSAKLAPDACFWKDRKLEP 765
             S      A A+          +GGYEPFVLTRCKSEPMRSSAKLAPD  F KDR LEP
Sbjct: 721 RWSCSFPAAAAAAAMIEQKLERAKGGYEPFVLTRCKSEPMRSSAKLAPDTGFCKDRNLEP 766

BLAST of Lag0019529 vs. ExPASy TrEMBL
Match: A0A6J1FMZ8 (glutamic acid-rich protein-like OS=Cucurbita moschata OX=3662 GN=LOC111446922 PE=4 SV=1)

HSP 1 Score: 904.8 bits (2337), Expect = 2.4e-259
Identity = 570/796 (71.61%), Postives = 628/796 (78.89%), Query Frame = 0

Query: 1   MDSERHFRTTSTNSTTSTATASSELFICFTSRFSSSSSSSMKISSKSILSPGRAREPSQI 60
           MDS+RHFR  +T S+T+TA+ASSELFICFTSRFSSSSSSSMKISSKSILSPGR REP+QI
Sbjct: 1   MDSDRHFR--NTTSSTATASASSELFICFTSRFSSSSSSSMKISSKSILSPGRPREPAQI 60

Query: 61  SLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKKQG 120
           SLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKKQG
Sbjct: 61  SLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKKQG 120

Query: 121 KKMRARSLKRRSNSEASFRKSES--VHSQVNGNDQQFVTKQSSHHNQHLLRQNSNSNGGN 180
           KKMRARSLKRRSNSEASFRKSES  V SQ+NGND      QSSH N HLLRQ  NSNGGN
Sbjct: 121 KKMRARSLKRRSNSEASFRKSESVQVQSQMNGND---FVNQSSHLNHHLLRQ--NSNGGN 180

Query: 181 GFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSDRENHNKESKPAERS- 240
           GFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSS+REN NKESK  ERS 
Sbjct: 181 GFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSNREN-NKESKTTERSS 240

Query: 241 -SESSCGTVFARWLVAVQDGDGKGREIELVVGDEEARTEKENGSQRRHVFEGMDFKEENE 300
            SESSCGTVFARWLVAVQD DG+GREIELVVGDEE+RTEKENGSQRRHVFEG+DFKEE E
Sbjct: 241 ESESSCGTVFARWLVAVQDNDGRGREIELVVGDEESRTEKENGSQRRHVFEGLDFKEEKE 300

Query: 301 VVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESPAPKVDEEDEEEEEEDEEK 360
           VVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESP PK++EED  EEE DEE 
Sbjct: 301 VVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESPVPKLEEED--EEENDEEN 360

Query: 361 NKQNEAKRDLPVPVLTPLTVTVTKEEEEEEKRVELNVKLKNEEEMSEEEFVSDAEVENQD 420
           + QNEA++  P+PVL PLTVT+ KEEEEEE +VELN KLKNEEEM EE  VSDAE E ++
Sbjct: 361 SNQNEAQKGTPLPVLMPLTVTLIKEEEEEETKVELNSKLKNEEEMIEES-VSDAE-EEEE 420

Query: 421 DVNLVLQEEEEDDE---EEAMEMATENEIDVQKLDITVVNQRQEEPADEEKEQE---EDE 480
           + N+VLQEEEE++E   EE++EMATENEIDVQKLDITV+N + +E A+E+KEQE   E  
Sbjct: 421 EANVVLQEEEEEEEDNGEESIEMATENEIDVQKLDITVINHQDQEEAEEDKEQEHEQEHR 480

Query: 481 IDQDNQQEKSSEETLPIPITIPTHCELEMARDAETLESIEEDESKLPHGNEQDQKREEDE 540
           IDQDNQQ+K  EET+   I I T CE EM +DAE LES E DE K  HGNEQD + EE  
Sbjct: 481 IDQDNQQQKLVEETMAFSIPISTQCEPEMVQDAEKLESAEGDEFKPFHGNEQDFETEEQM 540

Query: 541 DQRRELEDGENGENPTSPSLSVETERVLEEAETEVDGNWEEEEEESRGRSTEEEVKETTT 600
            +  E E  ENGENPTSP LSV         ETEVDGNW EEEEE+RGRSTEEE+K T  
Sbjct: 541 KELEEEEKSENGENPTSPPLSV---------ETEVDGNW-EEEEENRGRSTEEELKGTAA 600

Query: 601 KATDEGIGPDAQNDDEMG-PAKEDQSKERETPPPEPERETQTQTETKPETSVLPDCLLLM 660
            A DEGIGP  QNDDEMG   +EDQSKERETPPPEPERETQTQ  TKPE SVLPDCLLLM
Sbjct: 601 TAMDEGIGPHIQNDDEMGLEEEEDQSKERETPPPEPERETQTQ--TKPEASVLPDCLLLM 660

Query: 661 IFH-KVRSDEGEEGGSA---------TAAEEAGDEAGGEQHAGDGGGAAGEMVVFVSGG- 720
           ++  K+  +  +E             T  ++A      ++          +  V +  G 
Sbjct: 661 MYEPKLSMEVSKETWVCSTDFIRCVPTREKKAAPPPPPKKREIKTAEKNTQTQVAIQPGR 720

Query: 721 ----------GSGGGDDRAEASEGEGGYEPFVLTRCKSEPMRSSAKLAPDACFWKDRKLE 765
                      +   + + E ++G GGYEPFVLTRCKSEPMRSSAKLAPD  F KDR LE
Sbjct: 721 WSCSFPAAAAAAAMIEQKLERAKG-GGYEPFVLTRCKSEPMRSSAKLAPDTGFCKDRNLE 771

BLAST of Lag0019529 vs. ExPASy TrEMBL
Match: A0A6J1IN35 (calponin homology domain-containing protein DDB_G0272472-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478932 PE=4 SV=1)

HSP 1 Score: 884.8 bits (2285), Expect = 2.6e-253
Identity = 570/795 (71.70%), Postives = 620/795 (77.99%), Query Frame = 0

Query: 1   MDSERHFRTTSTNSTTSTATASSELFICFTSRF--SSSSSSSMKISSKSILSPGRAREPS 60
           MDS+RHFR  +T S+T++A+ASSELFICFTSR   SSSSSSSMKISSKSILSPGR REP+
Sbjct: 1   MDSDRHFR--NTTSSTASASASSELFICFTSRISSSSSSSSSMKISSKSILSPGRPREPA 60

Query: 61  QISLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKK 120
           QISLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGC FENPEPSSPKVTCIGQVRVKTKK
Sbjct: 61  QISLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCGFENPEPSSPKVTCIGQVRVKTKK 120

Query: 121 QGKKMRARSLKRRSNSEASFRKSES--VHSQVNGNDQQFVTKQSSHHNQHLLRQNSNSNG 180
           QGKKMRARSLKRRSNSEASFRKSES  V SQ+NGND      QSSHHN HLLRQ  NSNG
Sbjct: 121 QGKKMRARSLKRRSNSEASFRKSESVQVQSQMNGND---FMNQSSHHNHHLLRQ--NSNG 180

Query: 181 GNGFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSDRENHNKESKPAER 240
           GNGFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSS+REN NKESK  ER
Sbjct: 181 GNGFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSNREN-NKESKTTER 240

Query: 241 S--SESSCGTVFARWLVAVQDGDGKGREIELVVGDEEARTEKENGSQRRHVFEGMDFKEE 300
           S  SESSCGTVFARWLVAVQD DG+GREIELVVGDEE+RTEK+NGSQRRHVFEG+DFKEE
Sbjct: 241 SSESESSCGTVFARWLVAVQDNDGRGREIELVVGDEESRTEKDNGSQRRHVFEGLDFKEE 300

Query: 301 NEVVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESPAPKVDEEDEEEEEEDE 360
            EVVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESP PK+   +EEEEE+DE
Sbjct: 301 KEVVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESPVPKL---EEEEEEDDE 360

Query: 361 EKNKQNEAKRDLPVPVLTPLTVTVTKEEEEEEKRVELNVKLKNEEEMSEEEFVSDAEVEN 420
           E + QNEAK+  P+PVL PL+VT+ KEEEEE K VELN KLKNEEEM EE  VSDAE E 
Sbjct: 361 ENSNQNEAKKGAPLPVLVPLSVTLIKEEEEETK-VELNSKLKNEEEMIEES-VSDAEEE- 420

Query: 421 QDDVNLVLQEEEEDD--EEEAMEMATENEIDVQKLDITVVN-QRQEEPADEEKEQEEDEI 480
            ++ NLVLQEE+E++   EE++EMATENEIDVQKLDITV N Q QEE A+E+KEQE   I
Sbjct: 421 -EEANLVLQEEKEEEVNGEESIEMATENEIDVQKLDITVTNHQDQEEEAEEDKEQEH-RI 480

Query: 481 DQDNQQEKSSEETLPIPITIPTHCELEMARDAETLESIEEDESKLPHGNEQDQKREEDED 540
           DQDNQQ+K  EET+   I I T CE EM +DAE LES E DE K  HGNEQD + EE   
Sbjct: 481 DQDNQQQK-LEETMAFSIPISTRCEPEMVQDAEKLESAEGDEFKPSHGNEQDFETEEQMK 540

Query: 541 QRRELEDGENGENPTSPSLSVETERVLEEAETEVDGNW---EEEEEESRGRSTEEEVKET 600
           +  E E  ENGENPTSP LSV         ET VDGNW   EEEEEE+RGRST EE+K T
Sbjct: 541 ELEEEEKSENGENPTSPPLSV---------ETAVDGNWEEEEEEEEENRGRST-EELKGT 600

Query: 601 TTKATDEGIGPDAQNDDEMGPAKEDQSKERETPPPEPERETQTQTETKPETSVLPDCLLL 660
           T  A DEGIGP  QNDDEMG  +EDQSKERETPPPEPERETQTQ  TKPE SVLPDCLLL
Sbjct: 601 TATAMDEGIGPHIQNDDEMGLEEEDQSKERETPPPEPERETQTQ--TKPEASVLPDCLLL 660

Query: 661 MIFH-KVRSDEGEEGGSATA----------AEEAGDEAGGEQHAGDGGGAAGEMVVFVSG 720
           M++  K+  +  +E    +            + A   A  ++     G      V    G
Sbjct: 661 MMYEPKLSMEVSKETWVCSTDFIRCVPTREKKAAPPPAPKKREIKTAGKHTQTQVAIQPG 720

Query: 721 GGSGGGDDRAEAS--------EGEGGYEPFVLTRCKSEPMRSSAKLAPDACFWKDRKLEP 765
             S      A A+          +GGYEPFVLTRCKSEPMRSSAKLAPD  F KDR LEP
Sbjct: 721 RWSCSFPAAAAAAAMIEQKLERAKGGYEPFVLTRCKSEPMRSSAKLAPDTGFCKDRNLEP 766

BLAST of Lag0019529 vs. ExPASy TrEMBL
Match: A0A6J1IQ21 (eukaryotic translation initiation factor 5B-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478932 PE=4 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.7e-252
Identity = 572/807 (70.88%), Postives = 626/807 (77.57%), Query Frame = 0

Query: 1   MDSERHFRTTSTNSTTSTATASSELFICFTSRF--SSSSSSSMKISSKSILSPGRAREPS 60
           MDS+RHFR  +T S+T++A+ASSELFICFTSR   SSSSSSSMKISSKSILSPGR REP+
Sbjct: 1   MDSDRHFR--NTTSSTASASASSELFICFTSRISSSSSSSSSMKISSKSILSPGRPREPA 60

Query: 61  QISLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKK 120
           QISLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGC FENPEPSSPKVTCIGQVRVKTKK
Sbjct: 61  QISLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCGFENPEPSSPKVTCIGQVRVKTKK 120

Query: 121 QGKKMRARSLKRRSNSEASFRKSES--VHSQVNGNDQQFVTKQSSHHNQHLLRQNSNSNG 180
           QGKKMRARSLKRRSNSEASFRKSES  V SQ+NGND      QSSHHN HLLRQ  NSNG
Sbjct: 121 QGKKMRARSLKRRSNSEASFRKSESVQVQSQMNGND---FMNQSSHHNHHLLRQ--NSNG 180

Query: 181 GNGFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSDRENHNKESKPAER 240
           GNGFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSS+REN NKESK  ER
Sbjct: 181 GNGFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSNREN-NKESKTTER 240

Query: 241 S--SESSCGTVFARWLVAVQDGDGKGREIELVVGDEEARTEKENGSQRRHVFEGMDFKEE 300
           S  SESSCGTVFARWLVAVQD DG+GREIELVVGDEE+RTEK+NGSQRRHVFEG+DFKEE
Sbjct: 241 SSESESSCGTVFARWLVAVQDNDGRGREIELVVGDEESRTEKDNGSQRRHVFEGLDFKEE 300

Query: 301 NEVVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESPAPKVDEEDEEEEEEDE 360
            EVVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESP PK+   +EEEEE+DE
Sbjct: 301 KEVVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESPVPKL---EEEEEEDDE 360

Query: 361 EKNKQNEAKRDLPVPVLTPLTVTVTKEEEEEEKRVELNVKLKNEEEMSEEEFVSDAEVEN 420
           E + QNEAK+  P+PVL PL+VT+ KEEEEE K VELN KLKNEEEM EE  VSDAE E 
Sbjct: 361 ENSNQNEAKKGAPLPVLVPLSVTLIKEEEEETK-VELNSKLKNEEEMIEES-VSDAEEE- 420

Query: 421 QDDVNLVLQEEEEDD--EEEAMEMATENEIDVQKLDITVVN-QRQEEPADEEKEQEEDEI 480
            ++ NLVLQEE+E++   EE++EMATENEIDVQKLDITV N Q QEE A+E+KEQE   I
Sbjct: 421 -EEANLVLQEEKEEEVNGEESIEMATENEIDVQKLDITVTNHQDQEEEAEEDKEQEH-RI 480

Query: 481 DQDNQQEKSSEETLPIPITIPTHCELEMARDAETLESIEEDESKLPHGNEQD-------- 540
           DQDNQQ+K  EET+   I I T CE EM +DAE LES E DE K  HGNEQD        
Sbjct: 481 DQDNQQQK-LEETMAFSIPISTRCEPEMVQDAEKLESAEGDEFKPSHGNEQDFETEEHEE 540

Query: 541 -QKREEDEDQRRELED---GENGENPTSPSLSVETERVLEEAETEVDGNW---EEEEEES 600
            ++ EE ++Q +ELE+    ENGENPTSP LSV         ET VDGNW   EEEEEE+
Sbjct: 541 HEEHEEHKEQMKELEEEEKSENGENPTSPPLSV---------ETAVDGNWEEEEEEEEEN 600

Query: 601 RGRSTEEEVKETTTKATDEGIGPDAQNDDEMGPAKEDQSKERETPPPEPERETQTQTETK 660
           RGRST EE+K TT  A DEGIGP  QNDDEMG  +EDQSKERETPPPEPERETQTQ  TK
Sbjct: 601 RGRST-EELKGTTATAMDEGIGPHIQNDDEMGLEEEDQSKERETPPPEPERETQTQ--TK 660

Query: 661 PETSVLPDCLLLMIFH-KVRSDEGEEGGSATA----------AEEAGDEAGGEQHAGDGG 720
           PE SVLPDCLLLM++  K+  +  +E    +            + A   A  ++     G
Sbjct: 661 PEASVLPDCLLLMMYEPKLSMEVSKETWVCSTDFIRCVPTREKKAAPPPAPKKREIKTAG 720

Query: 721 GAAGEMVVFVSGGGSGGGDDRAEAS--------EGEGGYEPFVLTRCKSEPMRSSAKLAP 765
                 V    G  S      A A+          +GGYEPFVLTRCKSEPMRSSAKLAP
Sbjct: 721 KHTQTQVAIQPGRWSCSFPAAAAAAAMIEQKLERAKGGYEPFVLTRCKSEPMRSSAKLAP 778

BLAST of Lag0019529 vs. ExPASy TrEMBL
Match: A0A0A0L789 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G236550 PE=4 SV=1)

HSP 1 Score: 872.8 bits (2254), Expect = 1.0e-249
Identity = 562/806 (69.73%), Postives = 622/806 (77.17%), Query Frame = 0

Query: 1   MDSERHFRTTSTNSTTSTATASSELFICFTSRFSSSSSSSMKISSKSILSPGRAREPSQI 60
           MDS+ HFRTTSTNST+STAT SSELFICFTSRFSSSSSSSMKISSKSILSPGR REPSQI
Sbjct: 1   MDSDPHFRTTSTNSTSSTATPSSELFICFTSRFSSSSSSSMKISSKSILSPGRPREPSQI 60

Query: 61  SLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKKQG 120
           SLSTSLSRRLKSSGSLKGGQASPMFPTG KKRGCAF+NPEPSSPKVTCIGQVRVKTKKQG
Sbjct: 61  SLSTSLSRRLKSSGSLKGGQASPMFPTGRKKRGCAFDNPEPSSPKVTCIGQVRVKTKKQG 120

Query: 121 KKMRARSLKRRSNSEASFRKSESV--HSQVNGNDQQFVTKQSSHHNQHLLRQNSNSNGGN 180
           KKMRARS KRR+NSEASFR+SES+   SQ NG+DQQF    SSHHN HLLRQNSNSN GN
Sbjct: 121 KKMRARSQKRRTNSEASFRRSESLVQSSQGNGSDQQF----SSHHNHHLLRQNSNSNAGN 180

Query: 181 GFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSDRENHNKESKPAERS- 240
           GFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCS +REN NKESKPAERS 
Sbjct: 181 GFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSGNREN-NKESKPAERSS 240

Query: 241 -SESSCGTVFARWLVAVQDGDGKGREIELVVGDEEARTEKENGSQRRHVFEGMDFKEENE 300
            SESSCGTVFARWLVAVQDGDGKGREIELVVGDEE RTEKENGSQRRHVFEG+DFK++NE
Sbjct: 241 ESESSCGTVFARWLVAVQDGDGKGREIELVVGDEETRTEKENGSQRRHVFEGLDFKDKNE 300

Query: 301 VVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESPAPKVDEEDEEEEEEDEE- 360
            V+EEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCE PAPKVDEEDEE E+ED E 
Sbjct: 301 AVEEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCEPPAPKVDEEDEEGEDEDNEA 360

Query: 361 KNKQNEAKRDLPVPVLTPLTVT----VTKEEEEEEKRVELNVKLKNEEEMSEEEFVSDAE 420
           K +QNE KRD+ VPV + +TV       KEEE+E K  +L VKL+NEEEM+ EE VSDA+
Sbjct: 361 KKRQNEVKRDVSVPVSSIVTVNKEEEEVKEEEDERKVEQLIVKLENEEEMN-EECVSDAD 420

Query: 421 VENQDDVNLVLQ----EEEEDDEEEAMEMATENEIDVQKLDITVVNQRQEEPADEEKEQE 480
            E +++ NLVLQ    EEEED+EEE +EMATENEID QK DITVVNQ  +E A EEK  E
Sbjct: 421 KE-KEEANLVLQEEEREEEEDNEEETIEMATENEIDEQK-DITVVNQLNQEQALEEK--E 480

Query: 481 EDEIDQDNQQEKSSEETLPIPITIPTHCELEMARDAETLESIEEDESKLPHGNEQDQKRE 540
           ED+ DQ NQQE  +   +PIP+ I THCE EMA+D E LES+E++E KL H +EQDQK E
Sbjct: 481 EDKTDQVNQQETMA---IPIPLLIQTHCEPEMAQDVEKLESVEKEEPKLSHESEQDQKTE 540

Query: 541 ED--------EDQRRELEDGENGENPTSPSLSVETERVLEEAETEVDGNWEEEEEESRGR 600
           ED        E++  E E+GENGE  TSPSLSVETE V +E ETEVD N EEEEEE    
Sbjct: 541 EDENLREDKEEEEEEEGENGENGETTTSPSLSVETEPVSDETETEVDVNREEEEEE---- 600

Query: 601 STEEEVKETTTKATDEGIGPDAQNDDEMGPAKEDQSKERETPPPEPERETQTQTETKPET 660
             EEE      K TDEGIGPD +ND  +GP +EDQSKE ETPPPEPE E + + +T+ ET
Sbjct: 601 --EEE------KTTDEGIGPDDENDVLVGPEEEDQSKEGETPPPEPESEPKPERKTQTET 660

Query: 661 SVLPDCLLLMIFH-KVRSDEGEEGGSATA--------------AEEAGDEAGGEQHAGDG 720
           SVLPDCLLLM++  K+  +  +E    +A               ++       ++     
Sbjct: 661 SVLPDCLLLMMYEPKLSMEVSKETWVCSADFIRCVPTREKKAIGKDPPPPPPPKKRETKP 720

Query: 721 GGAAGEMVVFVSGGGSGGGDDRAEASEGE------GGYEPFVLTRCKSEPMRSSAKLAPD 765
                  VV  +          A A+  E       GYEPFVLTRCKSEPMRSSAKLAPD
Sbjct: 721 TDTTQTAVVQPARWSCSFPAAAAAAAMIEQKLVRAKGYEPFVLTRCKSEPMRSSAKLAPD 780

BLAST of Lag0019529 vs. ExPASy TrEMBL
Match: A0A1S3C2C2 (glutamic acid-rich protein isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496068 PE=4 SV=1)

HSP 1 Score: 869.0 bits (2244), Expect = 1.5e-248
Identity = 562/809 (69.47%), Postives = 624/809 (77.13%), Query Frame = 0

Query: 1   MDSERHFRTTSTNSTTSTATASSELFICFTSRFSSSSSSSMKISSKSILSPGRAREPSQI 60
           MD +RHFRTTSTNST+STAT SSELFICFTSRF  SSSSSMKISSKSILSPGR REPSQI
Sbjct: 1   MDPDRHFRTTSTNSTSSTATPSSELFICFTSRF--SSSSSMKISSKSILSPGRHREPSQI 60

Query: 61  SLSTSLSRRLKSSGSLKGGQASPMFPTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKKQG 120
           SLSTSLSRRLKSSGSLKGGQASPMFPTG KKRGCAF+NPEPSSPKVTCIGQVRVKTKKQG
Sbjct: 61  SLSTSLSRRLKSSGSLKGGQASPMFPTGRKKRGCAFDNPEPSSPKVTCIGQVRVKTKKQG 120

Query: 121 KKMRARSLKRRSNSEASFRKSESV--HSQVNGNDQQFVTKQSSHHNQHLLRQNSNSNGGN 180
           KKMRARS KRR+NSEASFR+SESV   SQVN NDQQF    SSHHN HLLRQNSNSN GN
Sbjct: 121 KKMRARSQKRRTNSEASFRRSESVVQSSQVNSNDQQF----SSHHNHHLLRQNSNSNAGN 180

Query: 181 GFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSDRENHNKESKPAERS- 240
           GFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCS +REN NKE KPAERS 
Sbjct: 181 GFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSGNREN-NKEPKPAERSS 240

Query: 241 -SESSCGTVFARWLVAVQDGDGKGREIELVVGDEEARTEKENGSQRRHVFEGMDFKEENE 300
            SESSCGTVFARWLVAVQDGDGKGREIELVVGDEE RTEKENGSQRRHVFEG+DFK++NE
Sbjct: 241 ESESSCGTVFARWLVAVQDGDGKGREIELVVGDEETRTEKENGSQRRHVFEGLDFKDKNE 300

Query: 301 VVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESPAPKVDEEDEE--EEEEDE 360
            V+EEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCE PAPKVDEEDEE  E+E++E
Sbjct: 301 AVEEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCEPPAPKVDEEDEEEGEDEDNE 360

Query: 361 EKNKQNEAKRDLPVPVLTPLTVTVTKEEEEEEKRVE-------LNVKLKNEEEMSEEEFV 420
            K ++NE KRD+ VPV + +TV   +EEEEEE++ E         VKL+NEEE++EE  V
Sbjct: 361 AKKRKNEVKRDVSVPVSSIITVNKEEEEEEEEEKEEDERKVEQFVVKLENEEEVNEES-V 420

Query: 421 SDAEVENQDDVNLVLQE---EEEDDEEEAMEMATENEIDVQKLDITVVNQRQEEPADEEK 480
           SD + E +++ NLVLQE   EE+D+EEE +EMATEN+ D QK DITVVNQ  +E A EEK
Sbjct: 421 SDEDKE-KEEANLVLQEEQREEKDNEEETIEMATEND-DEQKQDITVVNQLNQEQALEEK 480

Query: 481 EQEEDEIDQDNQQEKSSEETLPIPITIPTHCELEMARDAETLESIEEDESKLPHGNEQDQ 540
             EED+ DQ NQQ     ET+ IPI I THCE EMA+DAE LES+E++ESKL H +EQDQ
Sbjct: 481 --EEDKTDQVNQQ-----ETMAIPIPIQTHCEPEMAQDAEKLESVEKEESKLSHESEQDQ 540

Query: 541 KREED-------EDQRRELEDGENGENPTSPSLSVETERVLEEAETEVDGNWEEEEEESR 600
           K EED       E++  E E+GENGENPTSPSLSVET+ VL+E ETEVDG  EEEEEE  
Sbjct: 541 KTEEDEILREEKEEEEEEEEEGENGENPTSPSLSVETKPVLDETETEVDGKREEEEEEEE 600

Query: 601 GRSTEEEVKETTTKATDEGIGPDAQNDDEM-GPAKEDQSKERETPPPEPERETQTQTETK 660
               EEE      KATDEGIGPD +N+  + GP +EDQSKERETPPPEPE E + +T+T 
Sbjct: 601 ----EEE------KATDEGIGPDDENNGALVGPEEEDQSKERETPPPEPEPEPEGKTQT- 660

Query: 661 PETSVLPDCLLLMIFH-KVRSDEGEE--------------GGSATAAEEAGDEAGGEQHA 720
            ETSVLPDCLLLM++  K+  +  +E                  T   +       ++  
Sbjct: 661 -ETSVLPDCLLLMMYEPKLSMEVSKETWVCSADFIRCVPTREKKTVGRDPPPPPPPKKRE 720

Query: 721 GDGGGAAGEMVVFVSGGGSGGGDDRAEASEGE------GGYEPFVLTRCKSEPMRSSAKL 765
                     VV  +          A A+  E       GYEPFVLTRCKSEPMRSSAKL
Sbjct: 721 TKPTDTMQTTVVQPARWSCSFPAAAAAAAMIEQKLARAKGYEPFVLTRCKSEPMRSSAKL 780

BLAST of Lag0019529 vs. TAIR 10
Match: AT3G15095.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; Has 9762 Blast hits to 6439 proteins in 764 species: Archae - 77; Bacteria - 1339; Metazoa - 3211; Fungi - 718; Plants - 437; Viruses - 131; Other Eukaryotes - 3849 (source: NCBI BLink). )

HSP 1 Score: 243.0 bits (619), Expect = 7.6e-64
Identity = 293/816 (35.91%), Postives = 397/816 (48.65%), Query Frame = 0

Query: 2   DSERHFRTTSTNSTTSTATASS-ELFICFTSRFSSSSSSSMKISSKSILSPGRAREPSQI 61
           ++ER  R++S NS+++  + SS +LFICFTSRF  SSSSSM++SSKSI SP R+      
Sbjct: 3   ETERPHRSSSINSSSNNNSGSSTDLFICFTSRF--SSSSSMRLSSKSIHSPARS-----A 62

Query: 62  SLSTSLSRRLKSSGSLKGGQA----SPMF-PTGGKKR-GCAFENP--------EPSSPKV 121
            L+TSLSRRL++SGSLK   A    SPMF   GG+KR G  +EN         EPSSPKV
Sbjct: 63  CLTTSLSRRLRTSGSLKNASAGVLNSPMFGANGGRKRSGSGYENSNNNNNNNIEPSSPKV 122

Query: 122 TCIGQVRVKTKKQ-GKKMRARSLKRRSNSEASFRKSESVHSQVNGNDQQFVTKQSSHHNQ 181
           TCIGQVRVKT+K   KKMRARS  RR   E SFR+S      V+ ND             
Sbjct: 123 TCIGQVRVKTRKHVKKKMRARS--RRKGGENSFRRS------VDQND------------- 182

Query: 182 HLLRQNSNSNGGNGFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSDRE 241
                     GG G +         R VHLP TICE+LR+FG+ELNCF PC SSC+   E
Sbjct: 183 ----------GGGGCR---FKASENRLVHLPVTICESLRSFGSELNCFFPCRSSCT---E 242

Query: 242 NHNKESKPAERSSE---------SSCGTVFARWLVAVQD-GDGKGREIELVVGDEEARTE 301
           N + + + AE +++         +SCG VF RW VAV++   GK REIELVVG E+   E
Sbjct: 243 NSHGDGRRAESNNDGCGGGGGGSNSCGAVFTRWFVAVEETSGGKRREIELVVGGEDEVEE 302

Query: 302 KENGSQRRHVFEGMDFKE-----ENEVVQEEESRISICIPPKNALLLMRCRSDPVKMAEL 361
               S+RRHVFEG+D  E     E +   EE  R+SIC PPKNALLLMRCRSDPVK+A L
Sbjct: 303 DRRRSRRRHVFEGLDLSEIEMKTEKKERGEEVGRMSICSPPKNALLLMRCRSDPVKVAAL 362

Query: 362 AKRFCESPAPKVDEEDEEEEEEDEEKNKQNEAKRDLPVPVLTPLTVTVTKEEEEEEKRVE 421
           A R  E     +++    EEEEDE + +                     + E E++KR++
Sbjct: 363 ANRVRERQL-SLNDGVYTEEEEDERRRR--------------------FELEIEDKKRID 422

Query: 422 LNVKLKNEEEM--SEEEFVSDAEVENQDDVNLVLQEEEEDDEEEAMEMATENEIDVQKLD 481
           L  K  + E    +EE  V+ AE E + +    L      +EEE +++  ++ ++ ++  
Sbjct: 423 LCEKWISGETTVETEEVSVAVAEAEAEAEAEAPLPSNPATEEEERVKVVEDSIVEEEQEA 482

Query: 482 ITVVNQRQEEPADEEKEQEEDEIDQDNQQEKSSEETLPIPITIPTHCELEMARDAETLES 541
             +++  +EE      ++ EDEI    ++E+   E            EL +   AET E 
Sbjct: 483 SKILDSFEEEIEATIMKKIEDEIRNAIEEEEKLAEME----------ELAVVAVAET-EE 542

Query: 542 IEEDESKLPHGNEQDQKREEDEDQRRELEDGENGENPTSPSLSVETERVLEEAETEVDGN 601
           +EE +  +P    Q+++R             E G     PS  V   R L+E  TE +  
Sbjct: 543 VEESKEVVPDCIPQNEER------------SEQGNREPDPSPEVVMRRSLQEETTEKEKT 602

Query: 602 WEEEEE---------ESRGRSTEEEVKETTTKATD-----------EGIGPDAQNDDEMG 661
                +             + + E  KET   +TD           + I P+A  D+   
Sbjct: 603 TATPYKVLPDCLLLMMCEPKLSMEVSKETWVCSTDFVRCLPGRPPAKKIPPEAVGDNH-- 662

Query: 662 PAKEDQSKERETPPPEPERETQTQTETKPETSVLPDCLLLMIFHKVRSDEGEEGGSATAA 721
                Q K+R     +    ++ ++  +P   + P           RS            
Sbjct: 663 --HHHQPKKRIVTAVDSNASSRRRSIDRPPLHLQPP----------RSSCSYPAAPPIIT 684

Query: 722 EEAGDEAGGEQHAGDGGGAAGEMVVFVSGGGSGGGDDRAEASEGEGGYEPFVLTRCKSEP 765
             A   A GEQ                                G    +P VL RCKSEP
Sbjct: 723 AAA---AVGEQRVA-----------------------------GANKVQPPVLPRCKSEP 684

BLAST of Lag0019529 vs. TAIR 10
Match: AT3G15095.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; Has 9396 Blast hits to 6248 proteins in 757 species: Archae - 72; Bacteria - 1337; Metazoa - 3078; Fungi - 696; Plants - 406; Viruses - 135; Other Eukaryotes - 3672 (source: NCBI BLink). )

HSP 1 Score: 156.4 bits (394), Expect = 9.3e-38
Identity = 203/633 (32.07%), Postives = 289/633 (45.66%), Query Frame = 0

Query: 169 RQNSNSNGGNGFQQECLSHRNQRWVHLPFTICEALRAFGAELNCFLPCHSSCSSDRENHN 228
           R    ++GG G +         R VHLP TICE+LR+FG+ELNCF PC SSC+   EN +
Sbjct: 16  RSVDQNDGGGGCR---FKASENRLVHLPVTICESLRSFGSELNCFFPCRSSCT---ENSH 75

Query: 229 KESKPAERSSE---------SSCGTVFARWLVAVQD-GDGKGREIELVVGDEEARTEKEN 288
            + + AE +++         +SCG VF RW VAV++   GK REIELVVG E+   E   
Sbjct: 76  GDGRRAESNNDGCGGGGGGSNSCGAVFTRWFVAVEETSGGKRREIELVVGGEDEVEEDRR 135

Query: 289 GSQRRHVFEGMDFKE-----ENEVVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKR 348
            S+RRHVFEG+D  E     E +   EE  R+SIC PPKNALLLMRCRSDPVK+A LA R
Sbjct: 136 RSRRRHVFEGLDLSEIEMKTEKKERGEEVGRMSICSPPKNALLLMRCRSDPVKVAALANR 195

Query: 349 FCESPAPKVDEEDEEEEEEDEEKNKQNEAKRDLPVPVLTPLTVTVTKEEEEEEKRVELNV 408
             E     +++    EEEEDE + +                     + E E++KR++L  
Sbjct: 196 VRERQL-SLNDGVYTEEEEDERRRR--------------------FELEIEDKKRIDLCE 255

Query: 409 KLKNEEEM--SEEEFVSDAEVENQDDVNLVLQEEEEDDEEEAMEMATENEIDVQKLDITV 468
           K  + E    +EE  V+ AE E + +    L      +EEE +++  ++ ++ ++    +
Sbjct: 256 KWISGETTVETEEVSVAVAEAEAEAEAEAPLPSNPATEEEERVKVVEDSIVEEEQEASKI 315

Query: 469 VNQRQEEPADEEKEQEEDEIDQDNQQEKSSEETLPIPITIPTHCELEMARDAETLESIEE 528
           ++  +EE      ++ EDEI    ++E+   E            EL +   AET E +EE
Sbjct: 316 LDSFEEEIEATIMKKIEDEIRNAIEEEEKLAEME----------ELAVVAVAET-EEVEE 375

Query: 529 DESKLPHGNEQDQKREEDEDQRRELEDGENGENPTSPSLSVETERVLEEAETEVDGNWEE 588
            +  +P    Q+++R             E G     PS  V   R L+E  TE +     
Sbjct: 376 SKEVVPDCIPQNEER------------SEQGNREPDPSPEVVMRRSLQEETTEKEKTTAT 435

Query: 589 EEE---------ESRGRSTEEEVKETTTKATD-----------EGIGPDAQNDDEMGPAK 648
             +             + + E  KET   +TD           + I P+A  D+      
Sbjct: 436 PYKVLPDCLLLMMCEPKLSMEVSKETWVCSTDFVRCLPGRPPAKKIPPEAVGDNH----H 495

Query: 649 EDQSKERETPPPEPERETQTQTETKPETSVLPDCLLLMIFHKVRSDEGEEGGSATAAEEA 708
             Q K+R     +    ++ ++  +P   + P           RS              A
Sbjct: 496 HHQPKKRIVTAVDSNASSRRRSIDRPPLHLQPP----------RSSCSYPAAPPIITAAA 552

Query: 709 GDEAGGEQHAGDGGGAAGEMVVFVSGGGSGGGDDRAEASEGEGGYEPFVLTRCKSEPMRS 765
              A GEQ                                G    +P VL RCKSEP +S
Sbjct: 556 ---AVGEQRVA-----------------------------GANKVQPPVLPRCKSEPRKS 552

BLAST of Lag0019529 vs. TAIR 10
Match: AT3G15095.3 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 61.2 bits (147), Expect = 4.1e-09
Identity = 141/495 (28.48%), Postives = 208/495 (42.02%), Query Frame = 0

Query: 292 KEENEVVQEEESRISICIPPKNALLLMRCRSDPVKMAELAKRFCESPAPKVDEEDEEEEE 351
           K E +   EE  R+SIC PPKNALLLMRCRSDPVK+A LA R  E     +++    EEE
Sbjct: 2   KTEKKERGEEVGRMSICSPPKNALLLMRCRSDPVKVAALANRVRERQL-SLNDGVYTEEE 61

Query: 352 EDEEKNKQNEAKRDLPVPVLTPLTVTVTKEEEEEEKRVELNVKLKNEEEM--SEEEFVSD 411
           EDE + +                     + E E++KR++L  K  + E    +EE  V+ 
Sbjct: 62  EDERRRR--------------------FELEIEDKKRIDLCEKWISGETTVETEEVSVAV 121

Query: 412 AEVENQDDVNLVLQEEEEDDEEEAMEMATENEIDVQKLDITVVNQRQEEPADEEKEQEED 471
           AE E + +    L      +EEE +++  ++ ++ ++    +++  +EE      ++ ED
Sbjct: 122 AEAEAEAEAEAPLPSNPATEEEERVKVVEDSIVEEEQEASKILDSFEEEIEATIMKKIED 181

Query: 472 EIDQDNQQEKSSEETLPIPITIPTHCELEMARDAETLESIEEDESKLPHGNEQDQKREED 531
           EI    ++E+   E            EL +   AET E +EE +  +P    Q+++R   
Sbjct: 182 EIRNAIEEEEKLAEME----------ELAVVAVAET-EEVEESKEVVPDCIPQNEER--- 241

Query: 532 EDQRRELEDGENGENPTSPSLSVETERVLEEAETEVDGNWEEEEE---------ESRGRS 591
                     E G     PS  V   R L+E  TE +       +             + 
Sbjct: 242 ---------SEQGNREPDPSPEVVMRRSLQEETTEKEKTTATPYKVLPDCLLLMMCEPKL 301

Query: 592 TEEEVKETTTKATD-----------EGIGPDAQNDDEMGPAKEDQSKERETPPPEPERET 651
           + E  KET   +TD           + I P+A  D+        Q K+R     +    +
Sbjct: 302 SMEVSKETWVCSTDFVRCLPGRPPAKKIPPEAVGDNH----HHHQPKKRIVTAVDSNASS 361

Query: 652 QTQTETKPETSVLPDCLLLMIFHKVRSDEGEEGGSATAAEEAGDEAGGEQHAGDGGGAAG 711
           + ++  +P   + P           RS              A   A GEQ          
Sbjct: 362 RRRSIDRPPLHLQPP----------RSSCSYPAAPPIITAAA---AVGEQRVA------- 406

Query: 712 EMVVFVSGGGSGGGDDRAEASEGEGGYEPFVLTRCKSEPMRSSAKLAPDACFWKDRKLEP 765
                                 G    +P VL RCKSEP +S++KLAP+ACFWK+RKLEP
Sbjct: 422 ----------------------GANKVQPPVLPRCKSEPRKSASKLAPEACFWKNRKLEP 406

BLAST of Lag0019529 vs. TAIR 10
Match: AT1G78110.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 17 plant structures; EXPRESSED DURING: 7 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G22230.1); Has 5452 Blast hits to 3541 proteins in 289 species: Archae - 4; Bacteria - 165; Metazoa - 1756; Fungi - 532; Plants - 205; Viruses - 141; Other Eukaryotes - 2649 (source: NCBI BLink). )

HSP 1 Score: 52.8 bits (125), Expect = 1.5e-06
Identity = 90/362 (24.86%), Postives = 147/362 (40.61%), Query Frame = 0

Query: 13  NSTTSTATASSELFICFTSRFSSSSSSSMKISSKSILSPGRAREPSQISLSTSLSRRLKS 72
           N  +S++  S++L +CF SR      + + ++ K I SP R  + S         +  K 
Sbjct: 8   NRGSSSSGYSADLLVCFPSR------THLALTPKPICSPSRPSDSSTNRRPHHRRQLSKL 67

Query: 73  SGSLKGGQASPMF--PTGGKKRGCAFENPEPSSPKVTCIGQVRVKTKKQGKKMRARSLKR 132
           SG   GG  SP+        K     E  EP+SPKVTC GQ++V+  K G         R
Sbjct: 68  SGGGGGGHGSPVLWAKQASSKNMGGDEIAEPTSPKVTCAGQIKVRPSKCG--------GR 127

Query: 133 RSNSEASFRKSESVHSQVNGNDQQFVTKQSSHHNQHLLRQNSNSNGGNGFQQECLSHRNQ 192
             N ++   + E +H   N +  +F                       G +++ +     
Sbjct: 128 GKNWQSVMEEIERIHD--NRSQSKFF----------------------GLKKDVMG---- 187

Query: 193 RWVHLPFTICEALRAFGAELNCFLPC-HSSCSSD-----RENHNKESKPAERSSESSCGT 252
                 F  C  LR    +  CF    H+  +SD      ++ ++E +  E   E +  T
Sbjct: 188 ------FLTC--LRNIKFDFRCFGDFRHADVTSDDDEEEDDDDDEEEEVVEGEEEENSKT 247

Query: 253 VFARWLVAVQDGDGKGREIELVVGDEEARTEKENGSQRRHVFEGMDFKEENEVVQEEESR 312
           VF++W + +Q                E +  K++        E  D          E++ 
Sbjct: 248 VFSKWFMVLQ----------------EEQNNKDDDKNNNKCDEKRDL---------EDTE 293

Query: 313 ISICIPPKNALLLMRCRSDPVKMAELAKRFCESPAPKVDEEDEEEEEEDEEKNKQNEAKR 367
               +PP NALLLMRCRS P K     +   ++   K +E+ EE+E ED+E + + + K+
Sbjct: 308 TEPAVPPPNALLLMRCRSAPAKSWLEERMKVKTEQEKREEQKEEKETEDQETSMKTK-KK 293

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038894264.11.3e-26272.65glutamic acid-rich protein [Benincasa hispida][more]
XP_022941617.15.0e-25971.61glutamic acid-rich protein-like [Cucurbita moschata][more]
KAG7031049.13.2e-25871.15hypothetical protein SDJN02_05088, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023535806.16.3e-25470.79glutamic acid-rich protein-like [Cucurbita pepo subsp. pepo][more]
XP_022979182.15.3e-25371.70calponin homology domain-containing protein DDB_G0272472-like isoform X2 [Cucurb... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1FMZ82.4e-25971.61glutamic acid-rich protein-like OS=Cucurbita moschata OX=3662 GN=LOC111446922 PE... [more]
A0A6J1IN352.6e-25371.70calponin homology domain-containing protein DDB_G0272472-like isoform X2 OS=Cucu... [more]
A0A6J1IQ211.7e-25270.88eukaryotic translation initiation factor 5B-like isoform X1 OS=Cucurbita maxima ... [more]
A0A0A0L7891.0e-24969.73Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G236550 PE=4 SV=1[more]
A0A1S3C2C21.5e-24869.47glutamic acid-rich protein isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496068 PE... [more]
Match NameE-valueIdentityDescription
AT3G15095.17.6e-6435.91unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT3G15095.29.3e-3832.07unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT3G15095.34.1e-0928.48unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G78110.11.5e-0624.86unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 464..484
NoneNo IPR availableCOILSCoilCoilcoord: 379..401
NoneNo IPR availableCOILSCoilCoilcoord: 420..440
NoneNo IPR availableCOILSCoilCoilcoord: 341..365
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 341..355
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 497..541
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 657..714
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 337..644
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 572..593
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 35..182
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 35..80
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 461..476
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 402..438
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 378..401
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 139..182
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 605..633
NoneNo IPR availablePANTHERPTHR33448:SF4CHLOROPLAST PROTEIN HCF243coord: 714..764
NoneNo IPR availablePANTHERPTHR33448CHLOROPLAST PROTEIN HCF243-RELATEDcoord: 3..651
NoneNo IPR availablePANTHERPTHR33448CHLOROPLAST PROTEIN HCF243-RELATEDcoord: 714..764
NoneNo IPR availablePANTHERPTHR33448:SF4CHLOROPLAST PROTEIN HCF243coord: 3..651

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0019529.1Lag0019529.1mRNA