Lag0019528 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0019528 vs. NCBI nr
Match: XP_023553198.1 (reticulon-4-interacting protein 1, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 387.5 bits (994), Expect = 7.6e-104
Identity = 192/215 (89.30%), Postives = 205/215 (95.35%), Query Frame = 0

Query: 3   MEASAIPFAALTAWRALKSTARIKEGQRVLIIGGGGAVGSTAVQLSVASGCHVTTTCGSQ 62
           +EASAIPFAALTAWRALKSTARIKEGQRVL+IGGGGAVG  AVQ+SVASGCHV TTCGSQ
Sbjct: 150 VEASAIPFAALTAWRALKSTARIKEGQRVLVIGGGGAVGYAAVQISVASGCHVATTCGSQ 209

Query: 63  SIEKLLAAGAEQAIDYTTEGIEFIIKGKFDAVLDTIGEPDTERIGINLLNKGGHYMTLQG 122
           SI+KLLAAGAEQA+DYTTEGIEF +KGKFDAVLDTIG PDTERIGI+LLN+GGHYMTLQG
Sbjct: 210 SIDKLLAAGAEQAVDYTTEGIEFTLKGKFDAVLDTIGVPDTERIGIHLLNRGGHYMTLQG 269

Query: 123 EAASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTYMRADSEGLHEIRRLCDAGK 182
           EAASLTDRYGLVLGLPIATS+L++KQLQYR SH I YWWTYMRADSEGL EIRRLC+AGK
Sbjct: 270 EAASLTDRYGLVLGLPIATSILLQKQLQYRCSHGIEYWWTYMRADSEGLDEIRRLCEAGK 329

Query: 183 LKIPVEKTFPITQVREAHEAKDMNLIPGKVVLELD 218
           LKIPVEKTFPITQVREAHEAKD N+IPGKVVLELD
Sbjct: 330 LKIPVEKTFPITQVREAHEAKDKNIIPGKVVLELD 364

BLAST of Lag0019528 vs. NCBI nr
Match: KAG7015031.1 (Reticulon-4-interacting protein 1, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 387.5 bits (994), Expect = 7.6e-104
Identity = 192/215 (89.30%), Postives = 205/215 (95.35%), Query Frame = 0

Query: 3   MEASAIPFAALTAWRALKSTARIKEGQRVLIIGGGGAVGSTAVQLSVASGCHVTTTCGSQ 62
           +EASAIPFAALTAWRALKSTARIKEGQRVL+IGGGGAVG  AVQ+SVASGCHV TTCGSQ
Sbjct: 150 VEASAIPFAALTAWRALKSTARIKEGQRVLVIGGGGAVGYAAVQISVASGCHVATTCGSQ 209

Query: 63  SIEKLLAAGAEQAIDYTTEGIEFIIKGKFDAVLDTIGEPDTERIGINLLNKGGHYMTLQG 122
           SI+KLLAAGAEQA+DYTTEGIEF +KGKFDAVLDTIG PDTERIGI+LLN+GGHYMTLQG
Sbjct: 210 SIDKLLAAGAEQAVDYTTEGIEFTLKGKFDAVLDTIGVPDTERIGIHLLNRGGHYMTLQG 269

Query: 123 EAASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTYMRADSEGLHEIRRLCDAGK 182
           EAASLTDRYGLVLGLPIATS+L++KQLQYR SH I YWWTYMRADSEGL EIRRLC+AGK
Sbjct: 270 EAASLTDRYGLVLGLPIATSILLQKQLQYRCSHGIEYWWTYMRADSEGLDEIRRLCEAGK 329

Query: 183 LKIPVEKTFPITQVREAHEAKDMNLIPGKVVLELD 218
           LKIPVEKTFPITQVREAHEAKD N+IPGKVVLELD
Sbjct: 330 LKIPVEKTFPITQVREAHEAKDKNIIPGKVVLELD 364

BLAST of Lag0019528 vs. NCBI nr
Match: XP_022922906.1 (reticulon-4-interacting protein 1, mitochondrial [Cucurbita moschata])

HSP 1 Score: 386.3 bits (991), Expect = 1.7e-103
Identity = 192/215 (89.30%), Postives = 204/215 (94.88%), Query Frame = 0

Query: 3   MEASAIPFAALTAWRALKSTARIKEGQRVLIIGGGGAVGSTAVQLSVASGCHVTTTCGSQ 62
           +EASAIPFAALTAWRALKSTARIKEGQRVL+IGGGGAVG  AVQ+SVASGCHV TTCGSQ
Sbjct: 150 VEASAIPFAALTAWRALKSTARIKEGQRVLVIGGGGAVGYAAVQISVASGCHVATTCGSQ 209

Query: 63  SIEKLLAAGAEQAIDYTTEGIEFIIKGKFDAVLDTIGEPDTERIGINLLNKGGHYMTLQG 122
           SI+KLLAAGAEQA+DYTTEGIEF +KGKFDAVLDTIG PDTERIGI+LLN+GGHYMTLQG
Sbjct: 210 SIDKLLAAGAEQAVDYTTEGIEFTLKGKFDAVLDTIGVPDTERIGIHLLNRGGHYMTLQG 269

Query: 123 EAASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTYMRADSEGLHEIRRLCDAGK 182
           EAASLTDRYGLVLGLPIATS+L++KQLQYR SH I YWWTYMRADSEGL EIRRLC+AGK
Sbjct: 270 EAASLTDRYGLVLGLPIATSILLQKQLQYRCSHGIEYWWTYMRADSEGLDEIRRLCEAGK 329

Query: 183 LKIPVEKTFPITQVREAHEAKDMNLIPGKVVLELD 218
           LKIPVEKTFPITQVREAHEAKD N IPGKVVLELD
Sbjct: 330 LKIPVEKTFPITQVREAHEAKDKNTIPGKVVLELD 364

BLAST of Lag0019528 vs. NCBI nr
Match: KAG6577009.1 (Reticulon-4-interacting protein 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 386.3 bits (991), Expect = 1.7e-103
Identity = 191/215 (88.84%), Postives = 205/215 (95.35%), Query Frame = 0

Query: 3   MEASAIPFAALTAWRALKSTARIKEGQRVLIIGGGGAVGSTAVQLSVASGCHVTTTCGSQ 62
           +EA+AIPFAALTAWRALKSTARIKEGQRVL+IGGGGAVG  AVQ+SVASGCHV TTCGSQ
Sbjct: 150 VEATAIPFAALTAWRALKSTARIKEGQRVLVIGGGGAVGYAAVQISVASGCHVATTCGSQ 209

Query: 63  SIEKLLAAGAEQAIDYTTEGIEFIIKGKFDAVLDTIGEPDTERIGINLLNKGGHYMTLQG 122
           SI+KLLAAGAEQA+DYTTEGIEF +KGKFDAVLDTIG PDTERIGI+LLN+GGHYMTLQG
Sbjct: 210 SIDKLLAAGAEQAVDYTTEGIEFTLKGKFDAVLDTIGVPDTERIGIHLLNRGGHYMTLQG 269

Query: 123 EAASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTYMRADSEGLHEIRRLCDAGK 182
           EAASLTDRYGLVLGLPIATS+L++KQLQYR SH I YWWTYMRADSEGL EIRRLC+AGK
Sbjct: 270 EAASLTDRYGLVLGLPIATSILLQKQLQYRCSHGIEYWWTYMRADSEGLDEIRRLCEAGK 329

Query: 183 LKIPVEKTFPITQVREAHEAKDMNLIPGKVVLELD 218
           LKIPVEKTFPITQVREAHEAKD N+IPGKVVLELD
Sbjct: 330 LKIPVEKTFPITQVREAHEAKDKNIIPGKVVLELD 364

BLAST of Lag0019528 vs. NCBI nr
Match: XP_038879486.1 (reticulon-4-interacting protein 1, mitochondrial [Benincasa hispida])

HSP 1 Score: 380.9 bits (977), Expect = 7.1e-102
Identity = 190/215 (88.37%), Postives = 203/215 (94.42%), Query Frame = 0

Query: 3   MEASAIPFAALTAWRALKSTARIKEGQRVLIIGGGGAVGSTAVQLSVASGCHVTTTCGSQ 62
           +EASAIPFAALTAWRALKSTARI EGQRVL+IGGGGAVG +A+QLSVASGCHVTTTCGSQ
Sbjct: 179 VEASAIPFAALTAWRALKSTARITEGQRVLVIGGGGAVGYSAIQLSVASGCHVTTTCGSQ 238

Query: 63  SIEKLLAAGAEQAIDYTTEGIEFIIKGKFDAVLDTIGEPDTERIGINLLNKGGHYMTLQG 122
           SI+KLLAAGAEQAIDYTTEGIEF IKGKFDAVLDTIG PDTERIGI+LLN+GGHYMTLQG
Sbjct: 239 SIDKLLAAGAEQAIDYTTEGIEFKIKGKFDAVLDTIGVPDTERIGIHLLNRGGHYMTLQG 298

Query: 123 EAASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTYMRADSEGLHEIRRLCDAGK 182
           EAASLTDRYGLVLGLP ATS+L++KQL+YR SH I YWWTYMRADSEGL EIRRLC+A K
Sbjct: 299 EAASLTDRYGLVLGLPFATSILLQKQLKYRWSHGIEYWWTYMRADSEGLEEIRRLCEAEK 358

Query: 183 LKIPVEKTFPITQVREAHEAKDMNLIPGKVVLELD 218
           LKIPVEKTFPITQVREAH+AKD NLIPGKVVLE D
Sbjct: 359 LKIPVEKTFPITQVREAHDAKDKNLIPGKVVLEFD 393

BLAST of Lag0019528 vs. ExPASy Swiss-Prot
Match: Q7T3C7 (Reticulon-4-interacting protein 1 homolog, mitochondrial OS=Danio rerio OX=7955 GN=rtn4ip1 PE=2 SV=2)

HSP 1 Score: 117.9 bits (294), Expect = 1.5e-25
Identity = 78/222 (35.14%), Postives = 123/222 (55.41%), Query Frame = 0

Query: 4   EASAIPFAALTAWRALKSTARIKEG----QRVLIIGGGGAVGSTAVQLSVASGCHVTTTC 63
           EA++IP+ A TAW A+ +T  + +     +RVLI+GG G VG+ A+Q+  A G HVT TC
Sbjct: 163 EAASIPYVAATAWSAIVNTGGLNKDNSAKKRVLILGGSGGVGTFAIQMVKAWGAHVTVTC 222

Query: 64  GSQSIEKLLA-AGAEQAIDYTTEGIEFIIKG--KFDAVLDTIGEPDTERIGINLLN--KG 123
            SQ+ E+L+   GA+  +DYT   +E  +K   KFD +LD+IG  +TE+  ++LL    G
Sbjct: 223 -SQNAERLVRDLGADDVVDYTAGPVEKQLKNLEKFDLILDSIG-GETEKWALDLLKPWSG 282

Query: 124 GHYMTLQGEAASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTYMRADSEGLHEI 183
             ++TL       TDR GL  G+ + +++ V  ++       + Y W +       L E+
Sbjct: 283 AKFVTLITPFLQNTDRLGLADGM-MQSAVTVGCKVVKNLRKGVHYRWGFFAPSGSALDEV 342

Query: 184 RRLCDAGKLKIPVEKTFPITQVREAHEAKDMNLIPGKVVLEL 217
             + DAGK++  VE+ F   QV EA +  +     GK V+ +
Sbjct: 343 SEMVDAGKVRPVVEEVFSFAQVPEAFQKVEQGHARGKTVVSI 381

BLAST of Lag0019528 vs. ExPASy Swiss-Prot
Match: Q924D0 (Reticulon-4-interacting protein 1, mitochondrial OS=Mus musculus OX=10090 GN=Rtn4ip1 PE=1 SV=2)

HSP 1 Score: 115.9 bits (289), Expect = 5.6e-25
Identity = 74/221 (33.48%), Postives = 113/221 (51.13%), Query Frame = 0

Query: 4   EASAIPFAALTAWRALKSTARIKE----GQRVLIIGGGGAVGSTAVQLSVASGCHVTTTC 63
           +A+++P+ ALTAW A+     + +    G+R LI+G  G VG+ A+Q+  A G HVT  C
Sbjct: 177 QAASLPYVALTAWSAINKVGGLSDKNCKGKRALILGASGGVGTFAIQVMKAWGAHVTAVC 236

Query: 64  GSQSIEKLLAAGAEQAIDYTTEGIEFIIKG--KFDAVLDTIGEPDTERIGINLLNK--GG 123
              + E +   GA++ IDYT   +E  +K    FD +LD +G   TE   +N L K  G 
Sbjct: 237 SKDASELVRKLGADEVIDYTLGSVEEQLKSLKLFDFILDNVG-GSTETWALNFLKKWSGA 296

Query: 124 HYMTLQGEAASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTYMRADSEGLHEIR 183
            Y+TL        DR G+  G+ + T + V  +        + Y W +  A    L EI 
Sbjct: 297 TYVTLVTPFLLNMDRLGVADGM-LQTGVTVGTKALKHLWQGVHYRWAFFMASGPYLDEIA 356

Query: 184 RLCDAGKLKIPVEKTFPITQVREAHEAKDMNLIPGKVVLEL 217
            L DAGK++  +E+TFP ++V EA    +     GK V+ +
Sbjct: 357 ELVDAGKIRPVIERTFPFSEVPEAFLKVERGHARGKTVVNV 395

BLAST of Lag0019528 vs. ExPASy Swiss-Prot
Match: Q0VC50 (Reticulon-4-interacting protein 1, mitochondrial OS=Bos taurus OX=9913 GN=RTN4IP1 PE=2 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.8e-23
Identity = 71/221 (32.13%), Postives = 111/221 (50.23%), Query Frame = 0

Query: 4   EASAIPFAALTAWRALKSTARIKE----GQRVLIIGGGGAVGSTAVQLSVASGCHVTTTC 63
           +A+++P+ ALTAW A+     + +    G+RVLI+G  G VG+ A+Q+  A   HVT  C
Sbjct: 177 QAASLPYVALTAWSAINKVGGLNDRNCTGKRVLILGASGGVGTFAIQVMKAWDAHVTAVC 236

Query: 64  GSQSIEKLLAAGAEQAIDYTTEGIEFIIKGK--FDAVLDTIGEPDTERIGINLLNK--GG 123
              + E +   GA+  IDY +  +E  +K    FD +LD +G   TE   +  L K  G 
Sbjct: 237 SQDASELVRKLGADDVIDYKSGNVEAQLKSSKPFDFILDNVG-GSTETWALKFLKKWSGA 296

Query: 124 HYMTLQGEAASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTYMRADSEGLHEIR 183
            Y+TL        DR G+  G+ + T + V  +        + Y W +  A    L +I 
Sbjct: 297 TYVTLVTPFLLNMDRLGIADGM-LQTGVTVGSKTLKHFWQGVHYRWAFFMASGPCLDDIA 356

Query: 184 RLCDAGKLKIPVEKTFPITQVREAHEAKDMNLIPGKVVLEL 217
            L +AGK++  +EKTFP ++V EA    +     GK V+ +
Sbjct: 357 ELVEAGKIQPVIEKTFPFSKVPEAFLKVERGHARGKTVINV 395

BLAST of Lag0019528 vs. ExPASy Swiss-Prot
Match: Q8WWV3 (Reticulon-4-interacting protein 1, mitochondrial OS=Homo sapiens OX=9606 GN=RTN4IP1 PE=1 SV=2)

HSP 1 Score: 108.6 bits (270), Expect = 8.9e-23
Identity = 71/221 (32.13%), Postives = 111/221 (50.23%), Query Frame = 0

Query: 4   EASAIPFAALTAWRALKSTARIKE----GQRVLIIGGGGAVGSTAVQLSVASGCHVTTTC 63
           +A+++P+ ALTAW A+     + +    G+RVLI+G  G VG+ A+Q+  A   HVT  C
Sbjct: 177 QAASLPYVALTAWSAINKVGGLNDKNCTGKRVLILGASGGVGTFAIQVMKAWDAHVTAVC 236

Query: 64  GSQSIEKLLAAGAEQAIDYTTEGIEFIIKG--KFDAVLDTIGEPDTERIGINLLNK--GG 123
              + E +   GA+  IDY +  +E  +K    FD +LD +G   TE    + L K  G 
Sbjct: 237 SQDASELVRKLGADDVIDYKSGSVEEQLKSLKPFDFILDNVG-GSTETWAPDFLKKWSGA 296

Query: 124 HYMTLQGEAASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTYMRADSEGLHEIR 183
            Y+TL        DR G+  G+ + T + V  +        + Y W +  A    L +I 
Sbjct: 297 TYVTLVTPFLLNMDRLGIADGM-LQTGVTVGSKALKHFWKGVHYRWAFFMASGPCLDDIA 356

Query: 184 RLCDAGKLKIPVEKTFPITQVREAHEAKDMNLIPGKVVLEL 217
            L DAGK++  +E+TFP ++V EA    +     GK V+ +
Sbjct: 357 ELVDAGKIRPVIEQTFPFSKVPEAFLKVERGHARGKTVINV 395

BLAST of Lag0019528 vs. ExPASy Swiss-Prot
Match: Q4W4Z2 (Reticulon-4-interacting protein 1, mitochondrial OS=Caenorhabditis elegans OX=6239 GN=rad-8 PE=2 SV=2)

HSP 1 Score: 74.7 bits (182), Expect = 1.4e-12
Identity = 63/225 (28.00%), Postives = 109/225 (48.44%), Query Frame = 0

Query: 3   MEASAIPFAALTAWRAL---KSTARIKEGQRVLIIGGGGAVGSTAVQLSVASGCH-VTTT 62
           + A+A+P+ A TA+ A    + + R  + QRVLI GG G VGS A+QL  A GC  +  T
Sbjct: 147 VSAAALPYVASTAYSAFTIARVSQRNAKQQRVLIHGGAGGVGSMAIQLLKAWGCEKIVAT 206

Query: 63  CGSQSIEKLLAAGAEQAIDYTTEGI--EFIIKGKFDAVLDTIGEPDTERIGINLLN--KG 122
           C   S + +   GA   +DYT++    E I    F+ +LDT+ +    +   N++   + 
Sbjct: 207 CAKGSFDIVKQLGA-IPVDYTSDQATQELIEHAPFEVILDTV-DSQLAKWSDNVMGVWRN 266

Query: 123 GHYMTLQGEAASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTY--MRADSEGLH 182
             ++++        D+ G+ LGL        ++  Q   SH    W++Y   R  S+ + 
Sbjct: 267 CVHVSIVSPLMREMDKNGVPLGLVTTAMKHFERSFQ---SHLRGRWFSYAFFRPSSDLMS 326

Query: 183 EIRRLCDAGKLKIPVEKTFPITQVREAHEAKDMNLIPGKVVLELD 218
           ++ R  + GK+   VE+     ++ +A+E        GK V++ D
Sbjct: 327 QLSRFAEDGKIVPVVEQVMGFEELEKAYEKVSQLNGRGKTVIKYD 366

BLAST of Lag0019528 vs. ExPASy TrEMBL
Match: A0A6J1E5E5 (reticulon-4-interacting protein 1, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111430742 PE=4 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 8.2e-104
Identity = 192/215 (89.30%), Postives = 204/215 (94.88%), Query Frame = 0

Query: 3   MEASAIPFAALTAWRALKSTARIKEGQRVLIIGGGGAVGSTAVQLSVASGCHVTTTCGSQ 62
           +EASAIPFAALTAWRALKSTARIKEGQRVL+IGGGGAVG  AVQ+SVASGCHV TTCGSQ
Sbjct: 150 VEASAIPFAALTAWRALKSTARIKEGQRVLVIGGGGAVGYAAVQISVASGCHVATTCGSQ 209

Query: 63  SIEKLLAAGAEQAIDYTTEGIEFIIKGKFDAVLDTIGEPDTERIGINLLNKGGHYMTLQG 122
           SI+KLLAAGAEQA+DYTTEGIEF +KGKFDAVLDTIG PDTERIGI+LLN+GGHYMTLQG
Sbjct: 210 SIDKLLAAGAEQAVDYTTEGIEFTLKGKFDAVLDTIGVPDTERIGIHLLNRGGHYMTLQG 269

Query: 123 EAASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTYMRADSEGLHEIRRLCDAGK 182
           EAASLTDRYGLVLGLPIATS+L++KQLQYR SH I YWWTYMRADSEGL EIRRLC+AGK
Sbjct: 270 EAASLTDRYGLVLGLPIATSILLQKQLQYRCSHGIEYWWTYMRADSEGLDEIRRLCEAGK 329

Query: 183 LKIPVEKTFPITQVREAHEAKDMNLIPGKVVLELD 218
           LKIPVEKTFPITQVREAHEAKD N IPGKVVLELD
Sbjct: 330 LKIPVEKTFPITQVREAHEAKDKNTIPGKVVLELD 364

BLAST of Lag0019528 vs. ExPASy TrEMBL
Match: A0A6J1JBD9 (reticulon-4-interacting protein 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111482921 PE=4 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 4.5e-102
Identity = 189/215 (87.91%), Postives = 202/215 (93.95%), Query Frame = 0

Query: 3   MEASAIPFAALTAWRALKSTARIKEGQRVLIIGGGGAVGSTAVQLSVASGCHVTTTCGSQ 62
           +EASAIPFAALTAWRALKSTARIKEGQRVL+IGGGGAVG  AVQ+SVASGCHV TTCGSQ
Sbjct: 150 VEASAIPFAALTAWRALKSTARIKEGQRVLVIGGGGAVGYAAVQISVASGCHVATTCGSQ 209

Query: 63  SIEKLLAAGAEQAIDYTTEGIEFIIKGKFDAVLDTIGEPDTERIGINLLNKGGHYMTLQG 122
           SI+KLLAAGAEQA+DYTTEGIEF ++GKFDAVLDTIG PDTERIGI+LLN+GGHYMTLQG
Sbjct: 210 SIDKLLAAGAEQAVDYTTEGIEFTMEGKFDAVLDTIGVPDTERIGIHLLNRGGHYMTLQG 269

Query: 123 EAASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTYMRADSEGLHEIRRLCDAGK 182
           EAASLTDRYGLV GLPIATS+L++KQLQYR SH I YWWTYMRADSEGL EIRRLC+AGK
Sbjct: 270 EAASLTDRYGLVFGLPIATSILLQKQLQYRCSHGIEYWWTYMRADSEGLDEIRRLCEAGK 329

Query: 183 LKIPVEKTFPITQVREAHEAKDMNLIPGKVVLELD 218
           LKIPVEKTFPITQV EAHEAKD N IPGKVVLELD
Sbjct: 330 LKIPVEKTFPITQVMEAHEAKDKNTIPGKVVLELD 364

BLAST of Lag0019528 vs. ExPASy TrEMBL
Match: A0A6J1CNW2 (reticulon-4-interacting protein 1 homolog, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111012829 PE=4 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 2.9e-101
Identity = 184/215 (85.58%), Postives = 201/215 (93.49%), Query Frame = 0

Query: 3   MEASAIPFAALTAWRALKSTARIKEGQRVLIIGGGGAVGSTAVQLSVASGCHVTTTCGSQ 62
           +EASAIPFAALTAWRALKSTARI +G RVL+IGGGGAVGS AVQ+SVASGCHVTTTCGSQ
Sbjct: 76  VEASAIPFAALTAWRALKSTARITQGHRVLVIGGGGAVGSAAVQISVASGCHVTTTCGSQ 135

Query: 63  SIEKLLAAGAEQAIDYTTEGIEFIIKGKFDAVLDTIGEPDTERIGINLLNKGGHYMTLQG 122
           SI+KLLAAGAEQA+DYTTE IE +IKGKFDAVLDTIG PDTERIGI+LL +GGHYMTLQG
Sbjct: 136 SIDKLLAAGAEQAVDYTTEDIELVIKGKFDAVLDTIGAPDTERIGISLLKRGGHYMTLQG 195

Query: 123 EAASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTYMRADSEGLHEIRRLCDAGK 182
           EAAS TD+YGL+ GLPIATS+L KKQ+QYR+SH I YWWTYMRADSEGLHEIR+LC+AGK
Sbjct: 196 EAASFTDKYGLLFGLPIATSVLAKKQIQYRYSHGIEYWWTYMRADSEGLHEIRKLCEAGK 255

Query: 183 LKIPVEKTFPITQVREAHEAKDMNLIPGKVVLELD 218
           LKIPVEKTFPI+QVREAH AKD NLIPGKVVLELD
Sbjct: 256 LKIPVEKTFPISQVREAHNAKDKNLIPGKVVLELD 290

BLAST of Lag0019528 vs. ExPASy TrEMBL
Match: A0A5D3CLG4 (Reticulon-4-interacting protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G001120 PE=4 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 3.8e-101
Identity = 188/215 (87.44%), Postives = 200/215 (93.02%), Query Frame = 0

Query: 3   MEASAIPFAALTAWRALKSTARIKEGQRVLIIGGGGAVGSTAVQLSVASGCHVTTTCGSQ 62
           +EASAIPFAALTAWRALKSTARI+EGQRVL+IGGGGAVG  AVQLSVASGCHV TTCGSQ
Sbjct: 151 VEASAIPFAALTAWRALKSTARIREGQRVLVIGGGGAVGYAAVQLSVASGCHVATTCGSQ 210

Query: 63  SIEKLLAAGAEQAIDYTTEGIEFIIKGKFDAVLDTIGEPDTERIGINLLNKGGHYMTLQG 122
           SIEKLLAAGAEQA+DYTTEGIEF IKGKFDAVLDTIG PDTERIGI LLN+GGHYMTLQG
Sbjct: 211 SIEKLLAAGAEQAVDYTTEGIEFKIKGKFDAVLDTIGVPDTERIGIYLLNRGGHYMTLQG 270

Query: 123 EAASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTYMRADSEGLHEIRRLCDAGK 182
           EAASLTDRYGLVLGLP +T +L++KQL+YR SH I YWWTYMRADSEGL EIRRLC+A K
Sbjct: 271 EAASLTDRYGLVLGLPFSTGVLLQKQLKYRFSHAIEYWWTYMRADSEGLEEIRRLCEAEK 330

Query: 183 LKIPVEKTFPITQVREAHEAKDMNLIPGKVVLELD 218
           LKIPVEKTFPITQVREAH+AKD NLIPGKVVLE D
Sbjct: 331 LKIPVEKTFPITQVREAHDAKDKNLIPGKVVLEFD 365

BLAST of Lag0019528 vs. ExPASy TrEMBL
Match: A0A5A7UZ93 (Reticulon-4-interacting protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold803G00370 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 8.5e-101
Identity = 187/215 (86.98%), Postives = 200/215 (93.02%), Query Frame = 0

Query: 3   MEASAIPFAALTAWRALKSTARIKEGQRVLIIGGGGAVGSTAVQLSVASGCHVTTTCGSQ 62
           +EASAIPFAALTAWRALKSTARI+EGQRVL+IGGGGAVG  AVQLSVASGCHV TTCGSQ
Sbjct: 151 VEASAIPFAALTAWRALKSTARIREGQRVLVIGGGGAVGYAAVQLSVASGCHVATTCGSQ 210

Query: 63  SIEKLLAAGAEQAIDYTTEGIEFIIKGKFDAVLDTIGEPDTERIGINLLNKGGHYMTLQG 122
           SIEKLLAAG+EQA+DYTTEGIEF IKGKFDAVLDTIG PDTERIGI LLN+GGHYMTLQG
Sbjct: 211 SIEKLLAAGSEQAVDYTTEGIEFKIKGKFDAVLDTIGVPDTERIGIYLLNRGGHYMTLQG 270

Query: 123 EAASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTYMRADSEGLHEIRRLCDAGK 182
           EAASLTDRYGLVLGLP +T +L++KQL+YR SH I YWWTYMRADSEGL EIRRLC+A K
Sbjct: 271 EAASLTDRYGLVLGLPFSTGVLLQKQLKYRFSHAIEYWWTYMRADSEGLEEIRRLCEAEK 330

Query: 183 LKIPVEKTFPITQVREAHEAKDMNLIPGKVVLELD 218
           LKIPVEKTFPITQVREAH+AKD NLIPGKVVLE D
Sbjct: 331 LKIPVEKTFPITQVREAHDAKDKNLIPGKVVLEFD 365

BLAST of Lag0019528 vs. TAIR 10
Match: AT3G15090.1 (GroES-like zinc-binding alcohol dehydrogenase family protein )

HSP 1 Score: 328.2 bits (840), Expect = 5.1e-90
Identity = 161/213 (75.59%), Postives = 186/213 (87.32%), Query Frame = 0

Query: 3   MEASAIPFAALTAWRALKSTARIKEGQRVLIIGGGGAVGSTAVQLSVASGCHVTTTCGSQ 62
           +EASAIPFAALTAWRALKS ARI EGQR+L+ GGGGAVG +A+QL+VASGCHVT +C  Q
Sbjct: 153 VEASAIPFAALTAWRALKSNARITEGQRLLVFGGGGAVGFSAIQLAVASGCHVTASCVGQ 212

Query: 63  SIEKLLAAGAEQAIDYTTEGIEFIIKGKFDAVLDTIGEPDTERIGINLLNKGGHYMTLQG 122
           + +++LAAGAEQA+DYTTE IE  +KGKFDAVLDTIG P+TERIGIN L KGG+YMTLQG
Sbjct: 213 TKDRILAAGAEQAVDYTTEDIELAVKGKFDAVLDTIGGPETERIGINFLRKGGNYMTLQG 272

Query: 123 EAASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTYMRADSEGLHEIRRLCDAGK 182
           EAASLTD+YG V+GLP+ATSLL+KK++QY++SH I YWWTYMRAD EGL EI+RL  AGK
Sbjct: 273 EAASLTDKYGFVVGLPLATSLLMKKKIQYQYSHGIDYWWTYMRADPEGLAEIQRLVGAGK 332

Query: 183 LKIPVEKTFPITQVREAHEAKDMNLIPGKVVLE 216
           LKIPVEKTFPIT V  AHEAK+   IPGKVVLE
Sbjct: 333 LKIPVEKTFPITDVVAAHEAKEKKQIPGKVVLE 365

BLAST of Lag0019528 vs. TAIR 10
Match: AT4G21580.1 (oxidoreductase, zinc-binding dehydrogenase family protein )

HSP 1 Score: 67.0 bits (162), Expect = 2.1e-11
Identity = 65/232 (28.02%), Postives = 101/232 (43.53%), Query Frame = 0

Query: 2   IMEASAIPFAALTAWRALKSTARIKEGQRVLIIGGGGAVGSTAVQLSVASGCHVTTTCGS 61
           + +A+A P  A T W  +    R+  G+  LI GG   +G+ A+Q++   G  V  T GS
Sbjct: 114 LKDAAAFPEVACTVWSTVFMMGRLSVGESFLIHGGSSGIGTFAIQIAKHLGVRVFVTAGS 173

Query: 62  QSIEKLLAA---GAEQAIDYTTEGIEFIIKGK-------FDAVLDTIGEPDTERIGINLL 121
              EKL A    GA+  I+Y TE  +F+ K K        D +LD IG P  ++  ++ L
Sbjct: 174 D--EKLAACKELGADVCINYKTE--DFVAKVKAETDGKGVDVILDCIGAPYLQK-NLDSL 233

Query: 122 NKGGH--YMTLQGEA------ASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTY 181
           N  G    + L G A      +SL  +   VLG  +       K +  R   +       
Sbjct: 234 NFDGRLCIIGLMGGANAEIKLSSLLPKRLTVLGAALRPRSPENKAVVVREVEK------- 293

Query: 182 MRADSEGLHEIRRLCDAGKLKIPVEKTFPITQVREAHEAKDMNLIPGKVVLE 216
                     +    +AGK+K  + K  P++Q  E H   + +   GK++LE
Sbjct: 294 ---------NVWPAIEAGKVKPVIYKYLPLSQAAEGHSLMESSNHIGKILLE 324

BLAST of Lag0019528 vs. TAIR 10
Match: AT4G21580.2 (oxidoreductase, zinc-binding dehydrogenase family protein )

HSP 1 Score: 67.0 bits (162), Expect = 2.1e-11
Identity = 65/232 (28.02%), Postives = 101/232 (43.53%), Query Frame = 0

Query: 2   IMEASAIPFAALTAWRALKSTARIKEGQRVLIIGGGGAVGSTAVQLSVASGCHVTTTCGS 61
           + +A+A P  A T W  +    R+  G+  LI GG   +G+ A+Q++   G  V  T GS
Sbjct: 81  LKDAAAFPEVACTVWSTVFMMGRLSVGESFLIHGGSSGIGTFAIQIAKHLGVRVFVTAGS 140

Query: 62  QSIEKLLAA---GAEQAIDYTTEGIEFIIKGK-------FDAVLDTIGEPDTERIGINLL 121
              EKL A    GA+  I+Y TE  +F+ K K        D +LD IG P  ++  ++ L
Sbjct: 141 D--EKLAACKELGADVCINYKTE--DFVAKVKAETDGKGVDVILDCIGAPYLQK-NLDSL 200

Query: 122 NKGGH--YMTLQGEA------ASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTY 181
           N  G    + L G A      +SL  +   VLG  +       K +  R   +       
Sbjct: 201 NFDGRLCIIGLMGGANAEIKLSSLLPKRLTVLGAALRPRSPENKAVVVREVEK------- 260

Query: 182 MRADSEGLHEIRRLCDAGKLKIPVEKTFPITQVREAHEAKDMNLIPGKVVLE 216
                     +    +AGK+K  + K  P++Q  E H   + +   GK++LE
Sbjct: 261 ---------NVWPAIEAGKVKPVIYKYLPLSQAAEGHSLMESSNHIGKILLE 291

BLAST of Lag0019528 vs. TAIR 10
Match: AT1G23740.1 (Oxidoreductase, zinc-binding dehydrogenase family protein )

HSP 1 Score: 66.2 bits (160), Expect = 3.6e-11
Identity = 58/215 (26.98%), Postives = 95/215 (44.19%), Query Frame = 0

Query: 4   EASAIPFAALTAWRALKSTARIKEGQRVLIIGGGGAVGSTAVQLS--VASGCHVTTTCGS 63
           +A+ +P A  TA   L  T     G+ +L++ G G VGS  +QL+  V     V  T  +
Sbjct: 204 QAAGLPLAIETADEGLVRT-EFSAGKSILVLNGAGGVGSLVIQLAKHVYGASKVAATAST 263

Query: 64  QSIEKLLAAGAEQAIDYTTEGIEFIIKGKFDAVLDTIGEPDTERIGINLLNKGGHYMTLQ 123
           + +E + + GA+ AIDYT E IE  +  K+D V D IG  D     + ++ +GG  + L 
Sbjct: 264 EKLELVRSLGADLAIDYTKENIE-DLPDKYDVVFDAIGMCDK---AVKVIKEGGKVVALT 323

Query: 124 GEAASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTYMRADSEGLHEIRRLCDAG 183
           G       R+       + TS                        + + L ++    ++G
Sbjct: 324 GAVTPPGFRF-------VVTS------------------------NGDVLKKLNPYIESG 382

Query: 184 KLKIPVEK--TFPITQVREAHEAKDMNLIPGKVVL 215
           K+K  V+    FP ++V +A    + N   GKVV+
Sbjct: 384 KVKPVVDPKGPFPFSRVADAFSYLETNHATGKVVV 382

BLAST of Lag0019528 vs. TAIR 10
Match: AT4G21580.3 (oxidoreductase, zinc-binding dehydrogenase family protein )

HSP 1 Score: 58.9 bits (141), Expect = 5.8e-09
Identity = 59/229 (25.76%), Postives = 96/229 (41.92%), Query Frame = 0

Query: 2   IMEASAIPFAALTAWRALKSTARIKEGQRVLIIGGGGAVGSTAVQLSVASGCHVTTTCGS 61
           + +A+A P  A T W  +    R+  G+  LI GG   +G+ A+Q++   G  V  T  +
Sbjct: 114 LKDAAAFPEVACTVWSTVFMMGRLSVGESFLIHGGSSGIGTFAIQIAKHLGVRVFVTAAA 173

Query: 62  QSIEKLLAAGAEQAIDYTTEGIEFIIKGK-------FDAVLDTIGEPDTERIGINLLNKG 121
                    GA+  I+Y TE  +F+ K K        D +LD IG P  ++  ++ LN  
Sbjct: 174 -----CKELGADVCINYKTE--DFVAKVKAETDGKGVDVILDCIGAPYLQK-NLDSLNFD 233

Query: 122 GH--YMTLQGEA------ASLTDRYGLVLGLPIATSLLVKKQLQYRHSHEIAYWWTYMRA 181
           G    + L G A      +SL  +   VLG  +       K +  R   +          
Sbjct: 234 GRLCIIGLMGGANAEIKLSSLLPKRLTVLGAALRPRSPENKAVVVREVEK---------- 293

Query: 182 DSEGLHEIRRLCDAGKLKIPVEKTFPITQVREAHEAKDMNLIPGKVVLE 216
                  +    +AGK+K  + K  P++Q  E H   + +   GK++LE
Sbjct: 294 ------NVWPAIEAGKVKPVIYKYLPLSQAAEGHSLMESSNHIGKILLE 318