Lag0019360 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0019360
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionaspartic proteinase CDR1-like
Locationchr5: 41449476 .. 41450777 (-)
RNA-Seq ExpressionLag0019360
SyntenyLag0019360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCTACTTCTATCAACTACTTCTATCTCCTCCTATTATTCTTCATCTTCCAAACAACCGCCATCCGCGGCGGCGGCGGCAATGGCGGCTTCACCGCCTCTCTCTTCCACCGCGATTCTCCACTCTCCCCACTTCACAACCCATCTCTCACTCACTACCACCGTCTCAATAACGCCTTCCGCCGCTCCTTCTCCCGTGCCGCCGCCCTTTTCCACCGTGCCGCCGTCGAAATCCAATCTCCGATCACCCCCGGTAGCGGCGAGTATATAATGTCGGTCTCCATCGGAACCCCGCCGGTGGATTTCGTCGCCATCGCCGACACCGGGAGCGATCTGACGTGGACCCAATGCTTGCCATGTCAGAAATGCTTCAACCAATCAAACCCCATTTTCAACCCTCTCCAATCCTCGTCATTCAGTCACGTGCCTTGCACGTCCAAAACTTGTCACGCGCTCGACGTCGTCGAGTGTGGGGTCCAAGGGGTTTGCGACTACAGCTACACGTACGGAGACCAAACCTACACGAAGGGGGACTTGGGAATCGATAAGATCACCTTCGGGTCATCTTCCGTGAGCACGGTCTTCGGATGCGGCCACGAGAGCGGCGGCGGGTTCGGCGTTGCCTCGGGGGTCATCGGACTCGGCGGTGGCGAACTCTCGTTAGTCTCTCAAATGAGTCAAACCGCCGCCGTCAGCCGGCAATTCTCCTACTGCTTGCCGACCGTGCTCAGTCATGCGAATGGCAAGATAAACTTCGGCCCAAACGCCGTCGTTTCCGCCCCGGGAGTGGTTTCGACACCACTGGTCTCCAAAATCCCCAACACGCTTTATTACATGACTCTCGAAGCCGTTTCTGTTGGCGACGAACGTCATGCGGTCGACATGTCGGCTGCGCAAGCACAAGGCAACATGATTATAGACACTGGGACGACATTGTCGTACCTTCCCAAGGAGTTGTACGACGGCGTCGTTTCGTCATTGGTGAAGGTCGTTCGAGCGAAGCGGGTGGAGGATCCCGGTGGCTTTTTTGGACTCTGCTTTGCTGCAGACGGCAGCGGCGATGACGGTGGCTTGGATGTTCCGGTCATCACCGCCCATTTCGCCGGCGGTGCCAATGTGGATTTTTTGCCGGTGAATACGTTTAATAAAGTGGCCGATAAAGTGAGTTGCTTGACGTTTACGCCGTCGTCGCCGTCGGATCCTTTTGGGATTTTGGGGAATTTGGCGCAGGCGAACTTCTTGATTGGATATGATTTGGAGCTCGGGAAACTGTCGTTCAAGCCAACTATATGTGCTTAG

mRNA sequence

ATGGCTGCTACTTCTATCAACTACTTCTATCTCCTCCTATTATTCTTCATCTTCCAAACAACCGCCATCCGCGGCGGCGGCGGCAATGGCGGCTTCACCGCCTCTCTCTTCCACCGCGATTCTCCACTCTCCCCACTTCACAACCCATCTCTCACTCACTACCACCGTCTCAATAACGCCTTCCGCCGCTCCTTCTCCCGTGCCGCCGCCCTTTTCCACCGTGCCGCCGTCGAAATCCAATCTCCGATCACCCCCGGTAGCGGCGAGTATATAATGTCGGTCTCCATCGGAACCCCGCCGGTGGATTTCGTCGCCATCGCCGACACCGGGAGCGATCTGACGTGGACCCAATGCTTGCCATGTCAGAAATGCTTCAACCAATCAAACCCCATTTTCAACCCTCTCCAATCCTCGTCATTCAGTCACGTGCCTTGCACGTCCAAAACTTGTCACGCGCTCGACGTCGTCGAGTGTGGGGTCCAAGGGGTTTGCGACTACAGCTACACGTACGGAGACCAAACCTACACGAAGGGGGACTTGGGAATCGATAAGATCACCTTCGGGTCATCTTCCGTGAGCACGGTCTTCGGATGCGGCCACGAGAGCGGCGGCGGGTTCGGCGTTGCCTCGGGGGTCATCGGACTCGGCGGTGGCGAACTCTCGTTAGTCTCTCAAATGAGTCAAACCGCCGCCGTCAGCCGGCAATTCTCCTACTGCTTGCCGACCGTGCTCAGTCATGCGAATGGCAAGATAAACTTCGGCCCAAACGCCGTCGTTTCCGCCCCGGGAGTGGTTTCGACACCACTGGTCTCCAAAATCCCCAACACGCTTTATTACATGACTCTCGAAGCCGTTTCTGTTGGCGACGAACGTCATGCGGTCGACATGTCGGCTGCGCAAGCACAAGGCAACATGATTATAGACACTGGGACGACATTGTCGTACCTTCCCAAGGAGTTGTACGACGGCGTCGTTTCGTCATTGGTGAAGGTCGTTCGAGCGAAGCGGGTGGAGGATCCCGGTGGCTTTTTTGGACTCTGCTTTGCTGCAGACGGCAGCGGCGATGACGGTGGCTTGGATGTTCCGGTCATCACCGCCCATTTCGCCGGCGGTGCCAATGTGGATTTTTTGCCGGTGAATACGTTTAATAAAGTGGCCGATAAAGTGAGTTGCTTGACGTTTACGCCGTCGTCGCCGTCGGATCCTTTTGGGATTTTGGGGAATTTGGCGCAGGCGAACTTCTTGATTGGATATGATTTGGAGCTCGGGAAACTGTCGTTCAAGCCAACTATATGTGCTTAG

Coding sequence (CDS)

ATGGCTGCTACTTCTATCAACTACTTCTATCTCCTCCTATTATTCTTCATCTTCCAAACAACCGCCATCCGCGGCGGCGGCGGCAATGGCGGCTTCACCGCCTCTCTCTTCCACCGCGATTCTCCACTCTCCCCACTTCACAACCCATCTCTCACTCACTACCACCGTCTCAATAACGCCTTCCGCCGCTCCTTCTCCCGTGCCGCCGCCCTTTTCCACCGTGCCGCCGTCGAAATCCAATCTCCGATCACCCCCGGTAGCGGCGAGTATATAATGTCGGTCTCCATCGGAACCCCGCCGGTGGATTTCGTCGCCATCGCCGACACCGGGAGCGATCTGACGTGGACCCAATGCTTGCCATGTCAGAAATGCTTCAACCAATCAAACCCCATTTTCAACCCTCTCCAATCCTCGTCATTCAGTCACGTGCCTTGCACGTCCAAAACTTGTCACGCGCTCGACGTCGTCGAGTGTGGGGTCCAAGGGGTTTGCGACTACAGCTACACGTACGGAGACCAAACCTACACGAAGGGGGACTTGGGAATCGATAAGATCACCTTCGGGTCATCTTCCGTGAGCACGGTCTTCGGATGCGGCCACGAGAGCGGCGGCGGGTTCGGCGTTGCCTCGGGGGTCATCGGACTCGGCGGTGGCGAACTCTCGTTAGTCTCTCAAATGAGTCAAACCGCCGCCGTCAGCCGGCAATTCTCCTACTGCTTGCCGACCGTGCTCAGTCATGCGAATGGCAAGATAAACTTCGGCCCAAACGCCGTCGTTTCCGCCCCGGGAGTGGTTTCGACACCACTGGTCTCCAAAATCCCCAACACGCTTTATTACATGACTCTCGAAGCCGTTTCTGTTGGCGACGAACGTCATGCGGTCGACATGTCGGCTGCGCAAGCACAAGGCAACATGATTATAGACACTGGGACGACATTGTCGTACCTTCCCAAGGAGTTGTACGACGGCGTCGTTTCGTCATTGGTGAAGGTCGTTCGAGCGAAGCGGGTGGAGGATCCCGGTGGCTTTTTTGGACTCTGCTTTGCTGCAGACGGCAGCGGCGATGACGGTGGCTTGGATGTTCCGGTCATCACCGCCCATTTCGCCGGCGGTGCCAATGTGGATTTTTTGCCGGTGAATACGTTTAATAAAGTGGCCGATAAAGTGAGTTGCTTGACGTTTACGCCGTCGTCGCCGTCGGATCCTTTTGGGATTTTGGGGAATTTGGCGCAGGCGAACTTCTTGATTGGATATGATTTGGAGCTCGGGAAACTGTCGTTCAAGCCAACTATATGTGCTTAG

Protein sequence

MAATSINYFYLLLLFFIFQTTAIRGGGGNGGFTASLFHRDSPLSPLHNPSLTHYHRLNNAFRRSFSRAAALFHRAAVEIQSPITPGSGEYIMSVSIGTPPVDFVAIADTGSDLTWTQCLPCQKCFNQSNPIFNPLQSSSFSHVPCTSKTCHALDVVECGVQGVCDYSYTYGDQTYTKGDLGIDKITFGSSSVSTVFGCGHESGGGFGVASGVIGLGGGELSLVSQMSQTAAVSRQFSYCLPTVLSHANGKINFGPNAVVSAPGVVSTPLVSKIPNTLYYMTLEAVSVGDERHAVDMSAAQAQGNMIIDTGTTLSYLPKELYDGVVSSLVKVVRAKRVEDPGGFFGLCFAADGSGDDGGLDVPVITAHFAGGANVDFLPVNTFNKVADKVSCLTFTPSSPSDPFGILGNLAQANFLIGYDLELGKLSFKPTICA
Homology
BLAST of Lag0019360 vs. NCBI nr
Match: KAA0044967.1 (putative aspartic protease [Cucumis melo var. makuwa])

HSP 1 Score: 607.8 bits (1566), Expect = 7.1e-170
Identity = 307/436 (70.41%), Postives = 355/436 (81.42%), Query Frame = 0

Query: 1   MAATSINYFYLLLLFFIFQTTAIRGGGGNGGFTASLFHRDSPLSPLHNPSLTHYHRLNNA 60
           M AT   +F L LL   F  T I    G+ GFT SLFHRDS LSPL   +L+HY RL+NA
Sbjct: 1   MVATISIFFLLFLLLISFSQTTII--NGDNGFTTSLFHRDSLLSPLEFSTLSHYDRLSNA 60

Query: 61  FRRSFSRAAALFHRA----AVEIQSPITPGSGEYIMSVSIGTPPVDFVAIADTGSDLTWT 120
           FRRS SR+AAL +RA    AV +QSPI PGSGEY+MSVSIGTPPVD++ +ADTGSDLTW 
Sbjct: 61  FRRSLSRSAALLNRAATSGAVGLQSPIAPGSGEYLMSVSIGTPPVDYIGLADTGSDLTWA 120

Query: 121 QCLPCQKCFNQSNPIFNPLQSSSFSHVPCTSKTCHALDVVECGVQGVCDYSYTYGDQTYT 180
           QCLPC KCF QS PIFNPL+S+SFSHVPC S+ C A+D   CGVQGVCDYSYTYGDQTYT
Sbjct: 121 QCLPCVKCFKQSRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYT 180

Query: 181 KGDLGIDKITFGSSSVSTVFGCGHESGGGFGVASGVIGLGGGELSLVSQMSQTAAVSRQF 240
           KGDLG++KIT GSSSV +V GCGHESGGGFG ASGVIGLGGG+LSLVSQMSQT+ +SR+F
Sbjct: 181 KGDLGLEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRF 240

Query: 241 SYCLPTVLSHANGKINFGPNAVVSAPGVVSTPLVSKIPNTLYYMTLEAVSVGDERHAVDM 300
           SYCLPT+LSHANGKINFG NAVVS PGVVSTPL+SK P T YY+TLEA+S+G+ERH    
Sbjct: 241 SYCLPTLLSHANGKINFGQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERH---- 300

Query: 301 SAAQAQGNMIIDTGTTLSYLPKELYDGVVSSLVKVVRAKRVEDPGGFFGLCFAADGSGDD 360
            A+  QGN+IID+GTTL+ LPKELYDGVVSSL+KVV+AKRV+DPG F+ LCF  DG    
Sbjct: 301 MASAKQGNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCF-DDGINVA 360

Query: 361 GGLDVPVITAHFAGGANVDFLPVNTFNKVADKVSCLTFTPSSPSDPFGILGNLAQANFLI 420
               +P+IT HF+GGANV+ LPVNTF KVA+ V+CLT T +SP+D FGI+GNLAQANFLI
Sbjct: 361 ASSGIPIITTHFSGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLI 420

Query: 421 GYDLELGKLSFKPTIC 433
           GYDLE  +LSFKPT+C
Sbjct: 421 GYDLEAKRLSFKPTVC 429

BLAST of Lag0019360 vs. NCBI nr
Match: XP_008452153.1 (PREDICTED: probable aspartic protease At2g35615 [Cucumis melo] >TYK16501.1 putative aspartic protease [Cucumis melo var. makuwa])

HSP 1 Score: 606.7 bits (1563), Expect = 1.6e-169
Identity = 308/434 (70.97%), Postives = 355/434 (81.80%), Query Frame = 0

Query: 3   ATSINYFYLLLLFFIFQTTAIRGGGGNGGFTASLFHRDSPLSPLHNPSLTHYHRLNNAFR 62
           A SI    +L L    QTT I    G+ GFT SLFHRDS LSPL   SL+HY RL+NAFR
Sbjct: 2   AASIFCRLILFLISFSQTTII---NGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLSNAFR 61

Query: 63  RSFSRAAALFHRA----AVEIQSPITPGSGEYIMSVSIGTPPVDFVAIADTGSDLTWTQC 122
           RS SR+AAL +RA    AV +QSPI PGSGEY+MSVSIGTPPVD++ +ADTGSDLTW QC
Sbjct: 62  RSLSRSAALLNRAATSGAVGLQSPIAPGSGEYLMSVSIGTPPVDYIGLADTGSDLTWAQC 121

Query: 123 LPCQKCFNQSNPIFNPLQSSSFSHVPCTSKTCHALDVVECGVQGVCDYSYTYGDQTYTKG 182
           LPC KCF QS PIFNPL+S+SFSHVPC S+ C A+D   CGVQGVCDYSYTYGDQTYTKG
Sbjct: 122 LPCVKCFKQSRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 181

Query: 183 DLGIDKITFGSSSVSTVFGCGHESGGGFGVASGVIGLGGGELSLVSQMSQTAAVSRQFSY 242
           DLG++KIT GSSSV +V GCGHESGGGFG ASGVIGLGGG+LSLVSQMSQT+ +SR+FSY
Sbjct: 182 DLGLEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSY 241

Query: 243 CLPTVLSHANGKINFGPNAVVSAPGVVSTPLVSKIPNTLYYMTLEAVSVGDERHAVDMSA 302
           CLPT+LSHANGKINFG NAVVS PGVVSTPL+SK P T YY+TLEA+S+G+ERH     A
Sbjct: 242 CLPTLLSHANGKINFGQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERH----MA 301

Query: 303 AQAQGNMIIDTGTTLSYLPKELYDGVVSSLVKVVRAKRVEDPGGFFGLCFAADGSGDDGG 362
           +  QGN+IID+GTTL+ LPKELYDGVVSSL+KVV+AKRV+DPG F+ LCF  DG      
Sbjct: 302 SAKQGNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCF-DDGINVAAS 361

Query: 363 LDVPVITAHFAGGANVDFLPVNTFNKVADKVSCLTFTPSSPSDPFGILGNLAQANFLIGY 422
             +P+ITAHF+GGANV+ LPVNTF KVA+ V+CLT T +SP+D FGI+GNLAQANFLIGY
Sbjct: 362 SGIPIITAHFSGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLIGY 421

Query: 423 DLELGKLSFKPTIC 433
           DLE  +LSFKPT+C
Sbjct: 422 DLEAKRLSFKPTVC 427

BLAST of Lag0019360 vs. NCBI nr
Match: XP_038889229.1 (aspartic proteinase CDR1-like [Benincasa hispida])

HSP 1 Score: 602.8 bits (1553), Expect = 2.3e-168
Identity = 311/438 (71.00%), Postives = 349/438 (79.68%), Query Frame = 0

Query: 3   ATSINYFYLLLLFFIFQTTAIRGGGGNGGFTASLFHRDSPLSPLHNPSLTHYHRLNNAFR 62
           AT   + YLLLL    Q T       + GFT SLFHRD   S     SL+HY RL NAF+
Sbjct: 2   ATISLFSYLLLLISFSQATI----NDDNGFTTSLFHRD---SLFQISSLSHYDRLTNAFQ 61

Query: 63  RSFSRAAALFHR-------AAVEIQSPITPGSGEYIMSVSIGTPPVDFVAIADTGSDLTW 122
           RSFSR+ AL +         A  +QSPI PGSGEY+M VSIGTPPVD++ IADTGSDL W
Sbjct: 62  RSFSRSTALINHVATVATTTAAVVQSPIGPGSGEYLMYVSIGTPPVDYIGIADTGSDLIW 121

Query: 123 TQCLPCQKCFNQSNPIFNPLQSSSFSHVPCTSKTCHALDVVECGVQGVCDYSYTYGDQTY 182
           TQCLPCQKCFNQS PIFNPL+SSS+  V CTS++CHAL+V  CGVQG+C+YSYTYGDQTY
Sbjct: 122 TQCLPCQKCFNQSRPIFNPLKSSSYHRVSCTSQSCHALNVAHCGVQGICNYSYTYGDQTY 181

Query: 183 TKGDLGIDKITFGSSSVSTVFGCGHESGGGFGVASGVIGLGGGELSLVSQMSQTAAVSRQ 242
           TKGDLG DKIT GSSSV++V GCGHESGGGFG ASGVIGLGGGELSLVSQMSQTAA+S+Q
Sbjct: 182 TKGDLGFDKITIGSSSVNSVIGCGHESGGGFGYASGVIGLGGGELSLVSQMSQTAAISQQ 241

Query: 243 FSYCLPTVLSHANGKINFGPNAVVSAPGVVSTPLVSKIPNTLYYMTLEAVSVGDERHAVD 302
           FSYCLPT+LSHANGKINFG NA++S PGVVSTPLV K P T YYMTLEA+S+G+ERH  D
Sbjct: 242 FSYCLPTLLSHANGKINFGQNAIISGPGVVSTPLVPKNPKTYYYMTLEAISIGNERHVAD 301

Query: 303 MSAAQAQGNMIIDTGTTLSYLPKELYDGVVSSLVKVVRAKRVEDPGGFFGLCFAADGSGD 362
           MS+   QGNMIID+GTTL+ LPKELYDGVVSSL+KVVRA+RVEDPG F GLCFA D   +
Sbjct: 302 MSS--KQGNMIIDSGTTLTILPKELYDGVVSSLLKVVRARRVEDPGHFLGLCFADD--SN 361

Query: 363 DGGLDVPVITAHFAGGANVDFLPVNTFNKVADKVSCLTFTPSSPSDPFGILGNLAQANFL 422
            GGL +P+ITAHFAGGA+V  LP NTF KVA  VSC T T + P D FGILGNLAQANFL
Sbjct: 362 SGGLGIPIITAHFAGGADVKLLPENTFMKVAKNVSCSTLTSAEPRDGFGILGNLAQANFL 421

Query: 423 IGYDLELGKLSFKPTICA 434
           IGYDLE  +LSFKPTICA
Sbjct: 422 IGYDLEARRLSFKPTICA 428

BLAST of Lag0019360 vs. NCBI nr
Match: XP_031739975.1 (probable aspartic protease At2g35615 [Cucumis sativus] >KAE8649216.1 hypothetical protein Csa_014406 [Cucumis sativus])

HSP 1 Score: 601.3 bits (1549), Expect = 6.7e-168
Identity = 302/436 (69.27%), Postives = 355/436 (81.42%), Query Frame = 0

Query: 1   MAATSINYFYLLLLFFIFQTTAIRGGGGNGGFTASLFHRDSPLSPLHNPSLTHYHRLNNA 60
           M AT   +F+L+LL   F  T I    G+ GFT SLFHRDS LSPL   SL+HY RL NA
Sbjct: 1   MVATISIFFHLILLLISFSQTTII--NGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNA 60

Query: 61  FRRSFSRAAALFHRA----AVEIQSPITPGSGEYIMSVSIGTPPVDFVAIADTGSDLTWT 120
           FRRS SR+A L +RA    A+++Q+P+TPGSGEY+MSVSIGTPPVD++ +ADTGSDL W 
Sbjct: 61  FRRSLSRSATLLNRAATNGALDLQAPLTPGSGEYLMSVSIGTPPVDYIGMADTGSDLMWA 120

Query: 121 QCLPCQKCFNQSNPIFNPLQSSSFSHVPCTSKTCHALDVVECGVQGVCDYSYTYGDQTYT 180
           QCLPC KC+ QS PIF+PL+S+SFSHVPC S+ C A+D   CG QGVCDYSYTYGDQTYT
Sbjct: 121 QCLPCLKCYKQSRPIFDPLKSTSFSHVPCNSQNCKAIDDSHCGAQGVCDYSYTYGDQTYT 180

Query: 181 KGDLGIDKITFGSSSVSTVFGCGHESGGGFGVASGVIGLGGGELSLVSQMSQTAAVSRQF 240
           KGDLG +KIT GSSSV +V GCGHESGGGFG ASGVIGLGGG+LSLVSQMSQT+ +SR+F
Sbjct: 181 KGDLGFEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRF 240

Query: 241 SYCLPTVLSHANGKINFGPNAVVSAPGVVSTPLVSKIPNTLYYMTLEAVSVGDERHAVDM 300
           SYCLPT+LSHANGKINFG NAVVS PGVVSTPL+SK P T YY+TLEA+S+G+ERH    
Sbjct: 241 SYCLPTLLSHANGKINFGQNAVVSGPGVVSTPLISKNPVTYYYVTLEAISIGNERH---- 300

Query: 301 SAAQAQGNMIIDTGTTLSYLPKELYDGVVSSLVKVVRAKRVEDPGGFFGLCFAADGSGDD 360
            A+  QGN+IID+GTTLS+LPKELYDGVVSSL+KVV+AKRV+DPG F+ LCF  DG    
Sbjct: 301 MASAKQGNVIIDSGTTLSFLPKELYDGVVSSLLKVVKAKRVKDPGNFWDLCF-DDGINVA 360

Query: 361 GGLDVPVITAHFAGGANVDFLPVNTFNKVADKVSCLTFTPSSPSDPFGILGNLAQANFLI 420
               +P+ITA F+GGANV+ LPVNTF KVA+ V+CLT TP+SP+D FGI+GNLA ANFLI
Sbjct: 361 TSSGIPIITAQFSGGANVNLLPVNTFQKVANNVNCLTLTPASPTDEFGIIGNLALANFLI 420

Query: 421 GYDLELGKLSFKPTIC 433
           GYDLE  +LSFKPT+C
Sbjct: 421 GYDLEAKRLSFKPTVC 429

BLAST of Lag0019360 vs. NCBI nr
Match: XP_008452152.1 (PREDICTED: probable aspartic protease At2g35615 [Cucumis melo] >TYK16500.1 putative aspartic protease [Cucumis melo var. makuwa])

HSP 1 Score: 595.5 bits (1534), Expect = 3.7e-166
Identity = 302/436 (69.27%), Postives = 350/436 (80.28%), Query Frame = 0

Query: 1   MAATSINYFYLLLLFFIFQTTAIRGGGGNGGFTASLFHRDSPLSPLHNPSLTHYHRLNNA 60
           MAAT   +F L LL   F  T I    G+ GFT SLFHRDS LSPL   +L+HY RL+NA
Sbjct: 1   MAATISIFFLLFLLLISFSQTTII--NGDNGFTTSLFHRDSLLSPLEFSTLSHYDRLSNA 60

Query: 61  FRRSFSRAAALFHR----AAVEIQSPITPGSGEYIMSVSIGTPPVDFVAIADTGSDLTWT 120
           FRRS SR+AAL +R     AV +QSPI PGSGEY+M VSIGTPPVD++ + DTGSDLTW 
Sbjct: 61  FRRSLSRSAALLNRTATSGAVGLQSPIAPGSGEYLMYVSIGTPPVDYIGMIDTGSDLTWA 120

Query: 121 QCLPCQKCFNQSNPIFNPLQSSSFSHVPCTSKTCHALDVVECGVQGVCDYSYTYGDQTYT 180
           QCLPC+KCF Q  PIFNPL+S+SFSHVPC S+ C A+D   CGVQGVCDYSYTYGDQTYT
Sbjct: 121 QCLPCRKCFLQLRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYT 180

Query: 181 KGDLGIDKITFGSSSVSTVFGCGHESGGGFGVASGVIGLGGGELSLVSQMSQTAAVSRQF 240
           KGDLG +KIT GSSSV +V GCGHESGGGFG ASGVIGLGGG+LSLVSQMSQT+ +SR+F
Sbjct: 181 KGDLGFEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRF 240

Query: 241 SYCLPTVLSHANGKINFGPNAVVSAPGVVSTPLVSKIPNTLYYMTLEAVSVGDERHAVDM 300
           SYCLP +L HANGKINF  NAVVS PGVVSTPL+SK P T YY+TLEA+S+G+ERH    
Sbjct: 241 SYCLPPLLGHANGKINFAQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERH---- 300

Query: 301 SAAQAQGNMIIDTGTTLSYLPKELYDGVVSSLVKVVRAKRVEDPGGFFGLCFAADGSGDD 360
            A+  QGN+IID+GTTL+ LPKELYDGVVSSL+KVV+AKRV+DPG F+ LCF  DG    
Sbjct: 301 MASAKQGNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCF-DDGINVA 360

Query: 361 GGLDVPVITAHFAGGANVDFLPVNTFNKVADKVSCLTFTPSSPSDPFGILGNLAQANFLI 420
               +P+ITAHF+GGANV+ LPVNTF KVA+ V+CLT T +SP+D FGI+GNLAQANFLI
Sbjct: 361 ASSGIPIITAHFSGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLI 420

Query: 421 GYDLELGKLSFKPTIC 433
           GYDLE  +LSFKPT+C
Sbjct: 421 GYDLEAKRLSFKPTVC 429

BLAST of Lag0019360 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 9.9e-90
Identity = 194/450 (43.11%), Postives = 276/450 (61.33%), Query Frame = 0

Query: 11  LLLLFFIFQTTAIRGGGGNGGFTASLFHRDSPLSPLHNPSLTHYHRLNNAFRRSFSRAAA 70
           +LL FF+F +  +   G    F+  L HRDSPLSP++NP +T   RLN AF RS SR+  
Sbjct: 5   ILLCFFLFFSVTLSSSGHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRR 64

Query: 71  LFHR-AAVEIQSPITPGSGEYIMSVSIGTPPVDFVAIADTGSDLTWTQCLPCQKCFNQSN 130
             H+ +  ++QS +    GE+ MS++IGTPP+   AIADTGSDLTW QC PCQ+C+ ++ 
Sbjct: 65  FNHQLSQTDLQSGLIGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKENG 124

Query: 131 PIFNPLQSSSFSHVPCTSKTCHALDVVECGV---QGVCDYSYTYGDQTYTKGDLGIDKIT 190
           PIF+  +SS++   PC S+ C AL   E G      +C Y Y+YGDQ+++KGD+  + ++
Sbjct: 125 PIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETVS 184

Query: 191 FGSSSVS------TVFGCGHESGGGFG-VASGVIGLGGGELSLVSQMSQTAAVSRQFSYC 250
             S+S S      TVFGCG+ +GG F    SG+IGLGGG LSL+SQ+   +++S++FSYC
Sbjct: 185 IDSASGSPVSFPGTVFGCGYNNGGTFDETGSGIIGLGGGHLSLISQLG--SSISKKFSYC 244

Query: 251 LPTVLSHANGK--INFGPNAVVSA----PGVVSTPLVSKIPNTLYYMTLEAVSVGDERHA 310
           L    +  NG   IN G N++ S+     GVVSTPLV K P T YY+TLEA+SVG ++  
Sbjct: 245 LSHKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLTLEAISVGKKKIP 304

Query: 311 VDMSA---------AQAQGNMIIDTGTTLSYLPKELYDGVVSSLVK-VVRAKRVEDPGGF 370
              S+         ++  GN+IID+GTTL+ L    +D   S++ + V  AKRV DP G 
Sbjct: 305 YTGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQGL 364

Query: 371 FGLCFAADGSGDDGGLDVPVITAHFAGGANVDFLPVNTFNKVADKVSCLTFTPSSPSDPF 430
              CF + GS + G   +P IT HF  GA+V   P+N F K+++ + CL+  P++     
Sbjct: 365 LSHCFKS-GSAEIG---LPEITVHFT-GADVRLSPINAFVKLSEDMVCLSMVPTT---EV 424

Query: 431 GILGNLAQANFLIGYDLELGKLSFKPTICA 434
            I GN AQ +FL+GYDLE   +SF+   C+
Sbjct: 425 AIYGNFAQMDFLVGYDLETRTVSFQHMDCS 444

BLAST of Lag0019360 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 2.1e-87
Identity = 188/420 (44.76%), Postives = 256/420 (60.95%), Query Frame = 0

Query: 31  GFTASLFHRDSPLSPLHNPSLTHYHRLNNAFRRSFSRAAALFHRA----AVEIQSPITPG 90
           GFTA L HRDSP SP +NP  T   RL NA  RS +R   +FH        + Q  +T  
Sbjct: 30  GFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSVNR---VFHFTEKDNTPQPQIDLTSN 89

Query: 91  SGEYIMSVSIGTPPVDFVAIADTGSDLTWTQCLPCQKCFNQSNPIFNPLQSSSFSHVPCT 150
           SGEY+M+VSIGTPP   +AIADTGSDL WTQC PC  C+ Q +P+F+P  SS++  V C+
Sbjct: 90  SGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDPLFDPKTSSTYKDVSCS 149

Query: 151 SKTCHALD-VVECGV-QGVCDYSYTYGDQTYTKGDLGIDKITFGSSSV------STVFGC 210
           S  C AL+    C      C YS +YGD +YTKG++ +D +T GSS        + + GC
Sbjct: 150 SSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLTLGSSDTRPMQLKNIIIGC 209

Query: 211 GHESGGGFG-VASGVIGLGGGELSLVSQMSQTAAVSRQFSYCLPTVLSHAN--GKINFGP 270
           GH + G F    SG++GLGGG +SL+ Q+    ++  +FSYCL  + S  +   KINFG 
Sbjct: 210 GHNNAGTFNKKGSGIVGLGGGPVSLIKQLGD--SIDGKFSYCLVPLTSKKDQTSKINFGT 269

Query: 271 NAVVSAPGVVSTPLVSKI-PNTLYYMTLEAVSVGDERHAVDMSAAQ-AQGNMIIDTGTTL 330
           NA+VS  GVVSTPL++K    T YY+TL+++SVG ++     S ++ ++GN+IID+GTTL
Sbjct: 270 NAIVSGSGVVSTPLIAKASQETFYYLTLKSISVGSKQIQYSGSDSESSEGNIIIDSGTTL 329

Query: 331 SYLPKELYDGVVSSLVKVVRAKRVEDPGGFFGLCFAADGSGDDGGLDVPVITAHFAGGAN 390
           + LP E Y  +  ++   + A++ +DP     LC++A G      L VPVIT HF  GA+
Sbjct: 330 TLLPTEFYSELEDAVASSIDAEKKQDPQSGLSLCYSATGD-----LKVPVITMHF-DGAD 389

Query: 391 VDFLPVNTFNKVADKVSCLTFTPSSPSDPFGILGNLAQANFLIGYDLELGKLSFKPTICA 434
           V     N F +V++ + C  F   SPS  F I GN+AQ NFL+GYD     +SFKPT CA
Sbjct: 390 VKLDSSNAFVQVSEDLVCFAFR-GSPS--FSIYGNVAQMNFLVGYDTVSKTVSFKPTDCA 435

BLAST of Lag0019360 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.3e-60
Identity = 156/413 (37.77%), Postives = 208/413 (50.36%), Query Frame = 0

Query: 31  GFTASLFHRDSPLSPLHNPSLTHYHRLNNAFRRSFSRAAAL--FHRAAVEIQSPITPGSG 90
           GF   L H DS        +LT +  L  A  R   R   L         +++ +  G G
Sbjct: 40  GFQIMLEHVDS------GKNLTKFQLLERAIERGSRRLQRLEAMLNGPSGVETSVYAGDG 99

Query: 91  EYIMSVSIGTPPVDFVAIADTGSDLTWTQCLPCQKCFNQSNPIFNPLQSSSFSHVPCTSK 150
           EY+M++SIGTP   F AI DTGSDL WTQC PC +CFNQS PIFNP  SSSFS +PC+S+
Sbjct: 100 EYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQ 159

Query: 151 TCHALDVVECGVQGVCDYSYTYGDQTYTKGDLGIDKITFGSSSVSTV-FGCGHESGG-GF 210
            C AL    C     C Y+Y YGD + T+G +G + +TFGS S+  + FGCG  + G G 
Sbjct: 160 LCQALSSPTCS-NNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGCGENNQGFGQ 219

Query: 211 GVASGVIGLGGGELSLVSQMSQTAAVSRQFSYCLPTVLSHANGKINFGPNAVVSAPGVVS 270
           G  +G++G+G G LSL SQ+  T     +FSYC+  + S     +  G  A     G  +
Sbjct: 220 GNGAGLVGMGRGPLSLPSQLDVT-----KFSYCMTPIGSSTPSNLLLGSLANSVTAGSPN 279

Query: 271 TPLV--SKIPNTLYYMTLEAVSVGDERHAVDMSA-----AQAQGNMIIDTGTTLSYLPKE 330
           T L+  S+IP T YY+TL  +SVG  R  +D SA         G +IID+GTTL+Y    
Sbjct: 280 TTLIQSSQIP-TFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNN 339

Query: 331 LYDGVVSSLVKVVRAKRVEDPGGFFGLCFAADGSGDDGGLDVPVITAHFAGGANVDFLPV 390
            Y  V    +  +    V      F LCF      D   L +P    HF GG +++    
Sbjct: 340 AYQSVRQEFISQINLPVVNGSSSGFDLCFQT--PSDPSNLQIPTFVMHFDGG-DLELPSE 399

Query: 391 NTFNKVADKVSCLTFTPSSPSDPFGILGNLAQANFLIGYDLELGKLSFKPTIC 433
           N F   ++ + CL     S S    I GN+ Q N L+ YD     +SF    C
Sbjct: 400 NYFISPSNGLICLAM--GSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of Lag0019360 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 6.9e-59
Identity = 148/392 (37.76%), Postives = 209/392 (53.32%), Query Frame = 0

Query: 50  SLTHYHRLNNAFRRSFSRAAAL--FHRAAVEIQSPITPGSGEYIMSVSIGTPPVDFVAIA 109
           +LT Y  +  A +R   R  ++    +++  I++P+  G GEY+M+V+IGTP   F AI 
Sbjct: 54  NLTKYELIKRAIKRGERRMRSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIM 113

Query: 110 DTGSDLTWTQCLPCQKCFNQSNPIFNPLQSSSFSHVPCTSKTCHALDVVECGVQGVCDYS 169
           DTGSDL WTQC PC +CF+Q  PIFNP  SSSFS +PC S+ C  L    C     C Y+
Sbjct: 114 DTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCN-NNECQYT 173

Query: 170 YTYGDQTYTKGDLGIDKITFGSSSVSTV-FGCGHESGG-GFGVASGVIGLGGGELSLVSQ 229
           Y YGD + T+G +  +  TF +SSV  + FGCG ++ G G G  +G+IG+G G LSL SQ
Sbjct: 174 YGYGDGSTTQGYMATETFTFETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQ 233

Query: 230 MSQTAAVSRQFSYCLPTVLSHANGKINFGPNAVVSAPGVVSTPLV-SKIPNTLYYMTLEA 289
           +        QFSYC+ +  S +   +  G  A     G  ST L+ S +  T YY+TL+ 
Sbjct: 234 LG-----VGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSLNPTYYYITLQG 293

Query: 290 VSVGDERHAVDMSAAQAQ----GNMIIDTGTTLSYLPKELYDGVVSSLVKVVRAKRVEDP 349
           ++VG +   +  S  Q Q    G MIID+GTTL+YLP++ Y+ V  +    +    V++ 
Sbjct: 294 ITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDES 353

Query: 350 GGFFGLCFAADGSGDDGGLDVPVITAHFAGGANVDFLPVNTFNKVADKVSCLTFTPSSPS 409
                 CF     G    + VP I+  F GG  ++    N     A+ V CL    SS  
Sbjct: 354 SSGLSTCFQQPSDGST--VQVPEISMQFDGGV-LNLGEQNILISPAEGVICLAMGSSSQL 413

Query: 410 DPFGILGNLAQANFLIGYDLELGKLSFKPTIC 433
               I GN+ Q    + YDL+   +SF PT C
Sbjct: 414 G-ISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of Lag0019360 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 2.5e-48
Identity = 122/363 (33.61%), Postives = 184/363 (50.69%), Query Frame = 0

Query: 79  IQSPITPGSGEYIMSVSIGTPPVDFVAIADTGSDLTWTQCLPCQKCFNQSNPIFNPLQSS 138
           + S  + GSGEY   + +GTP  +   + DTGSD+ W QC PC  C+ QS+P+FNP  SS
Sbjct: 151 VVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSS 210

Query: 139 SFSHVPCTSKTCHALDVVECGVQGVCDYSYTYGDQTYTKGDLGIDKITFGSSSV--STVF 198
           ++  + C++  C  L+   C     C Y  +YGD ++T G+L  D +TFG+S    +   
Sbjct: 211 TYKSLTCSAPQCSLLETSACR-SNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVAL 270

Query: 199 GCGHESGGGFGVASGVIGLGGGELSLVSQMSQTAAVSRQFSYCLPTVLSHANGKINFGPN 258
           GCGH++ G F  A+G++GLGGG LS+ +QM  T+     FSYCL    S  +  ++F  N
Sbjct: 271 GCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATS-----FSYCLVDRDSGKSSSLDF--N 330

Query: 259 AVVSAPGVVSTPLV-SKIPNTLYYMTLEAVSVGDER----HAVDMSAAQAQGNMIIDTGT 318
           +V    G  + PL+ +K  +T YY+ L   SVG E+     A+    A   G +I+D GT
Sbjct: 331 SVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGT 390

Query: 319 TLSYLPKELYDGVVSSLVKV-VRAKRVEDPGGFFGLCFAADGSGDDGGLDVPVITAHFAG 378
            ++ L  + Y+ +  + +K+ V  K+       F  C+          + VP +  HF G
Sbjct: 391 AVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLST---VKVPTVAFHFTG 450

Query: 379 GANVDFLPVNTFNKVADK-VSCLTFTPSSPSDPFGILGNLAQANFLIGYDLELGKLSFKP 433
           G ++D    N    V D    C  F P+S S    I+GN+ Q    I YDL    +    
Sbjct: 451 GKSLDLPAKNYLIPVDDSGTFCFAFAPTSSS--LSIIGNVQQQGTRITYDLSKNVIGLSG 500

BLAST of Lag0019360 vs. ExPASy TrEMBL
Match: A0A5A7TSV3 (Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G002730 PE=3 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 3.5e-170
Identity = 307/436 (70.41%), Postives = 355/436 (81.42%), Query Frame = 0

Query: 1   MAATSINYFYLLLLFFIFQTTAIRGGGGNGGFTASLFHRDSPLSPLHNPSLTHYHRLNNA 60
           M AT   +F L LL   F  T I    G+ GFT SLFHRDS LSPL   +L+HY RL+NA
Sbjct: 1   MVATISIFFLLFLLLISFSQTTII--NGDNGFTTSLFHRDSLLSPLEFSTLSHYDRLSNA 60

Query: 61  FRRSFSRAAALFHRA----AVEIQSPITPGSGEYIMSVSIGTPPVDFVAIADTGSDLTWT 120
           FRRS SR+AAL +RA    AV +QSPI PGSGEY+MSVSIGTPPVD++ +ADTGSDLTW 
Sbjct: 61  FRRSLSRSAALLNRAATSGAVGLQSPIAPGSGEYLMSVSIGTPPVDYIGLADTGSDLTWA 120

Query: 121 QCLPCQKCFNQSNPIFNPLQSSSFSHVPCTSKTCHALDVVECGVQGVCDYSYTYGDQTYT 180
           QCLPC KCF QS PIFNPL+S+SFSHVPC S+ C A+D   CGVQGVCDYSYTYGDQTYT
Sbjct: 121 QCLPCVKCFKQSRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYT 180

Query: 181 KGDLGIDKITFGSSSVSTVFGCGHESGGGFGVASGVIGLGGGELSLVSQMSQTAAVSRQF 240
           KGDLG++KIT GSSSV +V GCGHESGGGFG ASGVIGLGGG+LSLVSQMSQT+ +SR+F
Sbjct: 181 KGDLGLEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRF 240

Query: 241 SYCLPTVLSHANGKINFGPNAVVSAPGVVSTPLVSKIPNTLYYMTLEAVSVGDERHAVDM 300
           SYCLPT+LSHANGKINFG NAVVS PGVVSTPL+SK P T YY+TLEA+S+G+ERH    
Sbjct: 241 SYCLPTLLSHANGKINFGQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERH---- 300

Query: 301 SAAQAQGNMIIDTGTTLSYLPKELYDGVVSSLVKVVRAKRVEDPGGFFGLCFAADGSGDD 360
            A+  QGN+IID+GTTL+ LPKELYDGVVSSL+KVV+AKRV+DPG F+ LCF  DG    
Sbjct: 301 MASAKQGNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCF-DDGINVA 360

Query: 361 GGLDVPVITAHFAGGANVDFLPVNTFNKVADKVSCLTFTPSSPSDPFGILGNLAQANFLI 420
               +P+IT HF+GGANV+ LPVNTF KVA+ V+CLT T +SP+D FGI+GNLAQANFLI
Sbjct: 361 ASSGIPIITTHFSGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLI 420

Query: 421 GYDLELGKLSFKPTIC 433
           GYDLE  +LSFKPT+C
Sbjct: 421 GYDLEAKRLSFKPTVC 429

BLAST of Lag0019360 vs. ExPASy TrEMBL
Match: A0A5D3CX41 (Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003090 PE=3 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 7.7e-170
Identity = 308/434 (70.97%), Postives = 355/434 (81.80%), Query Frame = 0

Query: 3   ATSINYFYLLLLFFIFQTTAIRGGGGNGGFTASLFHRDSPLSPLHNPSLTHYHRLNNAFR 62
           A SI    +L L    QTT I    G+ GFT SLFHRDS LSPL   SL+HY RL+NAFR
Sbjct: 2   AASIFCRLILFLISFSQTTII---NGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLSNAFR 61

Query: 63  RSFSRAAALFHRA----AVEIQSPITPGSGEYIMSVSIGTPPVDFVAIADTGSDLTWTQC 122
           RS SR+AAL +RA    AV +QSPI PGSGEY+MSVSIGTPPVD++ +ADTGSDLTW QC
Sbjct: 62  RSLSRSAALLNRAATSGAVGLQSPIAPGSGEYLMSVSIGTPPVDYIGLADTGSDLTWAQC 121

Query: 123 LPCQKCFNQSNPIFNPLQSSSFSHVPCTSKTCHALDVVECGVQGVCDYSYTYGDQTYTKG 182
           LPC KCF QS PIFNPL+S+SFSHVPC S+ C A+D   CGVQGVCDYSYTYGDQTYTKG
Sbjct: 122 LPCVKCFKQSRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 181

Query: 183 DLGIDKITFGSSSVSTVFGCGHESGGGFGVASGVIGLGGGELSLVSQMSQTAAVSRQFSY 242
           DLG++KIT GSSSV +V GCGHESGGGFG ASGVIGLGGG+LSLVSQMSQT+ +SR+FSY
Sbjct: 182 DLGLEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSY 241

Query: 243 CLPTVLSHANGKINFGPNAVVSAPGVVSTPLVSKIPNTLYYMTLEAVSVGDERHAVDMSA 302
           CLPT+LSHANGKINFG NAVVS PGVVSTPL+SK P T YY+TLEA+S+G+ERH     A
Sbjct: 242 CLPTLLSHANGKINFGQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERH----MA 301

Query: 303 AQAQGNMIIDTGTTLSYLPKELYDGVVSSLVKVVRAKRVEDPGGFFGLCFAADGSGDDGG 362
           +  QGN+IID+GTTL+ LPKELYDGVVSSL+KVV+AKRV+DPG F+ LCF  DG      
Sbjct: 302 SAKQGNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCF-DDGINVAAS 361

Query: 363 LDVPVITAHFAGGANVDFLPVNTFNKVADKVSCLTFTPSSPSDPFGILGNLAQANFLIGY 422
             +P+ITAHF+GGANV+ LPVNTF KVA+ V+CLT T +SP+D FGI+GNLAQANFLIGY
Sbjct: 362 SGIPIITAHFSGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLIGY 421

Query: 423 DLELGKLSFKPTIC 433
           DLE  +LSFKPT+C
Sbjct: 422 DLEAKRLSFKPTVC 427

BLAST of Lag0019360 vs. ExPASy TrEMBL
Match: A0A1S3BUB0 (probable aspartic protease At2g35615 OS=Cucumis melo OX=3656 GN=LOC103493257 PE=3 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 7.7e-170
Identity = 308/434 (70.97%), Postives = 355/434 (81.80%), Query Frame = 0

Query: 3   ATSINYFYLLLLFFIFQTTAIRGGGGNGGFTASLFHRDSPLSPLHNPSLTHYHRLNNAFR 62
           A SI    +L L    QTT I    G+ GFT SLFHRDS LSPL   SL+HY RL+NAFR
Sbjct: 2   AASIFCRLILFLISFSQTTII---NGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLSNAFR 61

Query: 63  RSFSRAAALFHRA----AVEIQSPITPGSGEYIMSVSIGTPPVDFVAIADTGSDLTWTQC 122
           RS SR+AAL +RA    AV +QSPI PGSGEY+MSVSIGTPPVD++ +ADTGSDLTW QC
Sbjct: 62  RSLSRSAALLNRAATSGAVGLQSPIAPGSGEYLMSVSIGTPPVDYIGLADTGSDLTWAQC 121

Query: 123 LPCQKCFNQSNPIFNPLQSSSFSHVPCTSKTCHALDVVECGVQGVCDYSYTYGDQTYTKG 182
           LPC KCF QS PIFNPL+S+SFSHVPC S+ C A+D   CGVQGVCDYSYTYGDQTYTKG
Sbjct: 122 LPCVKCFKQSRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 181

Query: 183 DLGIDKITFGSSSVSTVFGCGHESGGGFGVASGVIGLGGGELSLVSQMSQTAAVSRQFSY 242
           DLG++KIT GSSSV +V GCGHESGGGFG ASGVIGLGGG+LSLVSQMSQT+ +SR+FSY
Sbjct: 182 DLGLEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSY 241

Query: 243 CLPTVLSHANGKINFGPNAVVSAPGVVSTPLVSKIPNTLYYMTLEAVSVGDERHAVDMSA 302
           CLPT+LSHANGKINFG NAVVS PGVVSTPL+SK P T YY+TLEA+S+G+ERH     A
Sbjct: 242 CLPTLLSHANGKINFGQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERH----MA 301

Query: 303 AQAQGNMIIDTGTTLSYLPKELYDGVVSSLVKVVRAKRVEDPGGFFGLCFAADGSGDDGG 362
           +  QGN+IID+GTTL+ LPKELYDGVVSSL+KVV+AKRV+DPG F+ LCF  DG      
Sbjct: 302 SAKQGNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCF-DDGINVAAS 361

Query: 363 LDVPVITAHFAGGANVDFLPVNTFNKVADKVSCLTFTPSSPSDPFGILGNLAQANFLIGY 422
             +P+ITAHF+GGANV+ LPVNTF KVA+ V+CLT T +SP+D FGI+GNLAQANFLIGY
Sbjct: 362 SGIPIITAHFSGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLIGY 421

Query: 423 DLELGKLSFKPTIC 433
           DLE  +LSFKPT+C
Sbjct: 422 DLEAKRLSFKPTVC 427

BLAST of Lag0019360 vs. ExPASy TrEMBL
Match: A0A5D3CYR2 (Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003080 PE=3 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.8e-166
Identity = 302/436 (69.27%), Postives = 350/436 (80.28%), Query Frame = 0

Query: 1   MAATSINYFYLLLLFFIFQTTAIRGGGGNGGFTASLFHRDSPLSPLHNPSLTHYHRLNNA 60
           MAAT   +F L LL   F  T I    G+ GFT SLFHRDS LSPL   +L+HY RL+NA
Sbjct: 1   MAATISIFFLLFLLLISFSQTTII--NGDNGFTTSLFHRDSLLSPLEFSTLSHYDRLSNA 60

Query: 61  FRRSFSRAAALFHR----AAVEIQSPITPGSGEYIMSVSIGTPPVDFVAIADTGSDLTWT 120
           FRRS SR+AAL +R     AV +QSPI PGSGEY+M VSIGTPPVD++ + DTGSDLTW 
Sbjct: 61  FRRSLSRSAALLNRTATSGAVGLQSPIAPGSGEYLMYVSIGTPPVDYIGMIDTGSDLTWA 120

Query: 121 QCLPCQKCFNQSNPIFNPLQSSSFSHVPCTSKTCHALDVVECGVQGVCDYSYTYGDQTYT 180
           QCLPC+KCF Q  PIFNPL+S+SFSHVPC S+ C A+D   CGVQGVCDYSYTYGDQTYT
Sbjct: 121 QCLPCRKCFLQLRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYT 180

Query: 181 KGDLGIDKITFGSSSVSTVFGCGHESGGGFGVASGVIGLGGGELSLVSQMSQTAAVSRQF 240
           KGDLG +KIT GSSSV +V GCGHESGGGFG ASGVIGLGGG+LSLVSQMSQT+ +SR+F
Sbjct: 181 KGDLGFEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRF 240

Query: 241 SYCLPTVLSHANGKINFGPNAVVSAPGVVSTPLVSKIPNTLYYMTLEAVSVGDERHAVDM 300
           SYCLP +L HANGKINF  NAVVS PGVVSTPL+SK P T YY+TLEA+S+G+ERH    
Sbjct: 241 SYCLPPLLGHANGKINFAQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERH---- 300

Query: 301 SAAQAQGNMIIDTGTTLSYLPKELYDGVVSSLVKVVRAKRVEDPGGFFGLCFAADGSGDD 360
            A+  QGN+IID+GTTL+ LPKELYDGVVSSL+KVV+AKRV+DPG F+ LCF  DG    
Sbjct: 301 MASAKQGNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCF-DDGINVA 360

Query: 361 GGLDVPVITAHFAGGANVDFLPVNTFNKVADKVSCLTFTPSSPSDPFGILGNLAQANFLI 420
               +P+ITAHF+GGANV+ LPVNTF KVA+ V+CLT T +SP+D FGI+GNLAQANFLI
Sbjct: 361 ASSGIPIITAHFSGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLI 420

Query: 421 GYDLELGKLSFKPTIC 433
           GYDLE  +LSFKPT+C
Sbjct: 421 GYDLEAKRLSFKPTVC 429

BLAST of Lag0019360 vs. ExPASy TrEMBL
Match: A0A1S3BTZ4 (probable aspartic protease At2g35615 OS=Cucumis melo OX=3656 GN=LOC103493256 PE=3 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.8e-166
Identity = 302/436 (69.27%), Postives = 350/436 (80.28%), Query Frame = 0

Query: 1   MAATSINYFYLLLLFFIFQTTAIRGGGGNGGFTASLFHRDSPLSPLHNPSLTHYHRLNNA 60
           MAAT   +F L LL   F  T I    G+ GFT SLFHRDS LSPL   +L+HY RL+NA
Sbjct: 1   MAATISIFFLLFLLLISFSQTTII--NGDNGFTTSLFHRDSLLSPLEFSTLSHYDRLSNA 60

Query: 61  FRRSFSRAAALFHR----AAVEIQSPITPGSGEYIMSVSIGTPPVDFVAIADTGSDLTWT 120
           FRRS SR+AAL +R     AV +QSPI PGSGEY+M VSIGTPPVD++ + DTGSDLTW 
Sbjct: 61  FRRSLSRSAALLNRTATSGAVGLQSPIAPGSGEYLMYVSIGTPPVDYIGMIDTGSDLTWA 120

Query: 121 QCLPCQKCFNQSNPIFNPLQSSSFSHVPCTSKTCHALDVVECGVQGVCDYSYTYGDQTYT 180
           QCLPC+KCF Q  PIFNPL+S+SFSHVPC S+ C A+D   CGVQGVCDYSYTYGDQTYT
Sbjct: 121 QCLPCRKCFLQLRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYT 180

Query: 181 KGDLGIDKITFGSSSVSTVFGCGHESGGGFGVASGVIGLGGGELSLVSQMSQTAAVSRQF 240
           KGDLG +KIT GSSSV +V GCGHESGGGFG ASGVIGLGGG+LSLVSQMSQT+ +SR+F
Sbjct: 181 KGDLGFEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRF 240

Query: 241 SYCLPTVLSHANGKINFGPNAVVSAPGVVSTPLVSKIPNTLYYMTLEAVSVGDERHAVDM 300
           SYCLP +L HANGKINF  NAVVS PGVVSTPL+SK P T YY+TLEA+S+G+ERH    
Sbjct: 241 SYCLPPLLGHANGKINFAQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERH---- 300

Query: 301 SAAQAQGNMIIDTGTTLSYLPKELYDGVVSSLVKVVRAKRVEDPGGFFGLCFAADGSGDD 360
            A+  QGN+IID+GTTL+ LPKELYDGVVSSL+KVV+AKRV+DPG F+ LCF  DG    
Sbjct: 301 MASAKQGNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCF-DDGINVA 360

Query: 361 GGLDVPVITAHFAGGANVDFLPVNTFNKVADKVSCLTFTPSSPSDPFGILGNLAQANFLI 420
               +P+ITAHF+GGANV+ LPVNTF KVA+ V+CLT T +SP+D FGI+GNLAQANFLI
Sbjct: 361 ASSGIPIITAHFSGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLI 420

Query: 421 GYDLELGKLSFKPTIC 433
           GYDLE  +LSFKPT+C
Sbjct: 421 GYDLEAKRLSFKPTVC 429

BLAST of Lag0019360 vs. TAIR 10
Match: AT1G64830.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 335.9 bits (860), Expect = 4.9e-92
Identity = 188/417 (45.08%), Postives = 253/417 (60.67%), Query Frame = 0

Query: 31  GFTASLFHRDSPLSPLHNPSLTHYHRLNNAFRRSFSRAAALFHRAAVEIQSP---ITPGS 90
           GFT  L HRDSP SP +N + T   R+ NA RRS +R+   F        SP   IT   
Sbjct: 25  GFTIDLIHRDSPKSPFYNSAETSSQRMRNAIRRS-ARSTLQFSNDDASPNSPQSFITSNR 84

Query: 91  GEYIMSVSIGTPPVDFVAIADTGSDLTWTQCLPCQKCFNQSNPIFNPLQSSSFSHVPCTS 150
           GEY+M++SIGTPPV  +AIADTGSDL WTQC PC+ C+ Q++P+F+P +SS++  V C+S
Sbjct: 85  GEYLMNISIGTPPVPILAIADTGSDLIWTQCNPCEDCYQQTSPLFDPKESSTYRKVSCSS 144

Query: 151 KTCHALDVVECGV-QGVCDYSYTYGDQTYTKGDLGIDKITFGSSS------VSTVFGCGH 210
             C AL+   C   +  C Y+ TYGD +YTKGD+ +D +T GSS        + + GCGH
Sbjct: 145 SQCRALEDASCSTDENTCSYTITYGDNSYTKGDVAVDTVTMGSSGRRPVSLRNMIIGCGH 204

Query: 211 ESGGGFGVA-SGVIGLGGGELSLVSQMSQTAAVSRQFSYCLPTVLSHA--NGKINFGPNA 270
           E+ G F  A SG+IGLGGG  SLVSQ+ +  +++ +FSYCL    S      KINFG N 
Sbjct: 205 ENTGTFDPAGSGIIGLGGGSTSLVSQLRK--SINGKFSYCLVPFTSETGLTSKINFGTNG 264

Query: 271 VVSAPGVVSTPLVSKIPNTLYYMTLEAVSVGDER-HAVDMSAAQAQGNMIIDTGTTLSYL 330
           +VS  GVVST +V K P T Y++ LEA+SVG ++           +GN++ID+GTTL+ L
Sbjct: 265 IVSGDGVVSTSMVKKDPATYYFLNLEAISVGSKKIQFTSTIFGTGEGNIVIDSGTTLTLL 324

Query: 331 PKELYDGVVSSLVKVVRAKRVEDPGGFFGLCFAADGSGDDGGLDVPVITAHFAGGANVDF 390
           P   Y  + S +   ++A+RV+DP G   LC+      D     VP IT HF GG +V  
Sbjct: 325 PSNFYYELESVVASTIKAERVQDPDGILSLCYR-----DSSSFKVPDITVHFKGG-DVKL 384

Query: 391 LPVNTFNKVADKVSCLTFTPSSPSDPFGILGNLAQANFLIGYDLELGKLSFKPTICA 434
             +NTF  V++ VSC  F   + ++   I GNLAQ NFL+GYD   G +SFK T C+
Sbjct: 385 GNLNTFVAVSEDVSCFAF---AANEQLTIFGNLAQMNFLVGYDTVSGTVSFKKTDCS 429

BLAST of Lag0019360 vs. TAIR 10
Match: AT2G35615.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 332.0 bits (850), Expect = 7.0e-91
Identity = 194/450 (43.11%), Postives = 276/450 (61.33%), Query Frame = 0

Query: 11  LLLLFFIFQTTAIRGGGGNGGFTASLFHRDSPLSPLHNPSLTHYHRLNNAFRRSFSRAAA 70
           +LL FF+F +  +   G    F+  L HRDSPLSP++NP +T   RLN AF RS SR+  
Sbjct: 5   ILLCFFLFFSVTLSSSGHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRR 64

Query: 71  LFHR-AAVEIQSPITPGSGEYIMSVSIGTPPVDFVAIADTGSDLTWTQCLPCQKCFNQSN 130
             H+ +  ++QS +    GE+ MS++IGTPP+   AIADTGSDLTW QC PCQ+C+ ++ 
Sbjct: 65  FNHQLSQTDLQSGLIGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKENG 124

Query: 131 PIFNPLQSSSFSHVPCTSKTCHALDVVECGV---QGVCDYSYTYGDQTYTKGDLGIDKIT 190
           PIF+  +SS++   PC S+ C AL   E G      +C Y Y+YGDQ+++KGD+  + ++
Sbjct: 125 PIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETVS 184

Query: 191 FGSSSVS------TVFGCGHESGGGFG-VASGVIGLGGGELSLVSQMSQTAAVSRQFSYC 250
             S+S S      TVFGCG+ +GG F    SG+IGLGGG LSL+SQ+   +++S++FSYC
Sbjct: 185 IDSASGSPVSFPGTVFGCGYNNGGTFDETGSGIIGLGGGHLSLISQLG--SSISKKFSYC 244

Query: 251 LPTVLSHANGK--INFGPNAVVSA----PGVVSTPLVSKIPNTLYYMTLEAVSVGDERHA 310
           L    +  NG   IN G N++ S+     GVVSTPLV K P T YY+TLEA+SVG ++  
Sbjct: 245 LSHKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLTLEAISVGKKKIP 304

Query: 311 VDMSA---------AQAQGNMIIDTGTTLSYLPKELYDGVVSSLVK-VVRAKRVEDPGGF 370
              S+         ++  GN+IID+GTTL+ L    +D   S++ + V  AKRV DP G 
Sbjct: 305 YTGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQGL 364

Query: 371 FGLCFAADGSGDDGGLDVPVITAHFAGGANVDFLPVNTFNKVADKVSCLTFTPSSPSDPF 430
              CF + GS + G   +P IT HF  GA+V   P+N F K+++ + CL+  P++     
Sbjct: 365 LSHCFKS-GSAEIG---LPEITVHFT-GADVRLSPINAFVKLSEDMVCLSMVPTT---EV 424

Query: 431 GILGNLAQANFLIGYDLELGKLSFKPTICA 434
            I GN AQ +FL+GYDLE   +SF+   C+
Sbjct: 425 AIYGNFAQMDFLVGYDLETRTVSFQHMDCS 444

BLAST of Lag0019360 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 324.3 bits (830), Expect = 1.5e-88
Identity = 188/420 (44.76%), Postives = 256/420 (60.95%), Query Frame = 0

Query: 31  GFTASLFHRDSPLSPLHNPSLTHYHRLNNAFRRSFSRAAALFHRA----AVEIQSPITPG 90
           GFTA L HRDSP SP +NP  T   RL NA  RS +R   +FH        + Q  +T  
Sbjct: 30  GFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSVNR---VFHFTEKDNTPQPQIDLTSN 89

Query: 91  SGEYIMSVSIGTPPVDFVAIADTGSDLTWTQCLPCQKCFNQSNPIFNPLQSSSFSHVPCT 150
           SGEY+M+VSIGTPP   +AIADTGSDL WTQC PC  C+ Q +P+F+P  SS++  V C+
Sbjct: 90  SGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDPLFDPKTSSTYKDVSCS 149

Query: 151 SKTCHALD-VVECGV-QGVCDYSYTYGDQTYTKGDLGIDKITFGSSSV------STVFGC 210
           S  C AL+    C      C YS +YGD +YTKG++ +D +T GSS        + + GC
Sbjct: 150 SSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLTLGSSDTRPMQLKNIIIGC 209

Query: 211 GHESGGGFG-VASGVIGLGGGELSLVSQMSQTAAVSRQFSYCLPTVLSHAN--GKINFGP 270
           GH + G F    SG++GLGGG +SL+ Q+    ++  +FSYCL  + S  +   KINFG 
Sbjct: 210 GHNNAGTFNKKGSGIVGLGGGPVSLIKQLGD--SIDGKFSYCLVPLTSKKDQTSKINFGT 269

Query: 271 NAVVSAPGVVSTPLVSKI-PNTLYYMTLEAVSVGDERHAVDMSAAQ-AQGNMIIDTGTTL 330
           NA+VS  GVVSTPL++K    T YY+TL+++SVG ++     S ++ ++GN+IID+GTTL
Sbjct: 270 NAIVSGSGVVSTPLIAKASQETFYYLTLKSISVGSKQIQYSGSDSESSEGNIIIDSGTTL 329

Query: 331 SYLPKELYDGVVSSLVKVVRAKRVEDPGGFFGLCFAADGSGDDGGLDVPVITAHFAGGAN 390
           + LP E Y  +  ++   + A++ +DP     LC++A G      L VPVIT HF  GA+
Sbjct: 330 TLLPTEFYSELEDAVASSIDAEKKQDPQSGLSLCYSATGD-----LKVPVITMHF-DGAD 389

Query: 391 VDFLPVNTFNKVADKVSCLTFTPSSPSDPFGILGNLAQANFLIGYDLELGKLSFKPTICA 434
           V     N F +V++ + C  F   SPS  F I GN+AQ NFL+GYD     +SFKPT CA
Sbjct: 390 VKLDSSNAFVQVSEDLVCFAFR-GSPS--FSIYGNVAQMNFLVGYDTVSKTVSFKPTDCA 435

BLAST of Lag0019360 vs. TAIR 10
Match: AT1G31450.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 308.9 bits (790), Expect = 6.4e-84
Identity = 190/457 (41.58%), Postives = 262/457 (57.33%), Query Frame = 0

Query: 1   MAATSINYFYLLLLFFIFQTTAIRGGGGNGGFTASLFHRDSPLSPLHNPSLTHYHRLNNA 60
           MA  +  Y  LL + F F   A          T  L HRDSP SPL+NP  T   RLN A
Sbjct: 1   MATKTFLYCSLLAISFFF---ASNSSANRENLTVELIHRDSPHSPLYNPHHTVSDRLNAA 60

Query: 61  FRRSFSRAAALFHRAAVEIQSPITPGSGEYIMSVSIGTPPVDFVAIADTGSDLTWTQCLP 120
           F RS SR+         ++QS +    GEY MS+SIGTPP    AIADTGSDLTW QC P
Sbjct: 61  FLRSISRSRRF--TTKTDLQSGLISNGGEYFMSISIGTPPSKVFAIADTGSDLTWVQCKP 120

Query: 121 CQKCFNQSNPIFNPLQSSSFSHVPCTSKTCHALDVVECGV---QGVCDYSYTYGDQTYTK 180
           CQ+C+ Q++P+F+  +SS++    C SKTC AL   E G    + +C Y Y+YGD ++TK
Sbjct: 121 CQQCYKQNSPLFDKKKSSTYKTESCDSKTCQALSEHEEGCDESKDICKYRYSYGDNSFTK 180

Query: 181 GDLGIDKITFGSSSVS------TVFGCGHESGGGF-GVASGVIGLGGGELSLVSQMSQTA 240
           GD+  + I+  SSS S      TVFGCG+ +GG F    SG+IGLGGG LSLVSQ+   +
Sbjct: 181 GDVATETISIDSSSGSSVSFPGTVFGCGYNNGGTFEETGSGIIGLGGGPLSLVSQLG--S 240

Query: 241 AVSRQFSYCLPTVLSHANGK--INFGPNAVVSAP----GVVSTPLVSKIPNTLYYMTLEA 300
           ++ ++FSYCL    +  NG   IN G N++ S P      ++TPL+ K P T Y++TLEA
Sbjct: 241 SIGKKFSYCLSHTAATTNGTSVINLGTNSIPSNPSKDSATLTTPLIQKDPETYYFLTLEA 300

Query: 301 VSVGDER-------HAVDMSAAQAQGNMIIDTGTTLSYLPKELYDGVVSSLVK-VVRAKR 360
           V+VG  +       + ++  +++  GN+IID+GTTL+ L    YD   +++ + V  AKR
Sbjct: 301 VTVGKTKLPYTGGGYGLNGKSSKRTGNIIIDSGTTLTLLDSGFYDDFGTAVEESVTGAKR 360

Query: 361 VEDPGGFFGLCFAADGSGDDGGLDVPVITAHFAGGANVDFLPVNTFNKVADKVSCLTFTP 420
           V DP G    CF       D  + +P IT HF   A+V   P+N F K+ +   CL+  P
Sbjct: 361 VSDPQGLLTHCF----KSGDKEIGLPAITMHFT-NADVKLSPINAFVKLNEDTVCLSMIP 420

Query: 421 SSPSDPFGILGNLAQANFLIGYDLELGKLSFKPTICA 434
           ++      I GN+ Q +FL+GYDLE   +SF+   C+
Sbjct: 421 TT---EVAIYGNMVQMDFLVGYDLETKTVSFQRMDCS 442

BLAST of Lag0019360 vs. TAIR 10
Match: AT2G28010.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 213.4 bits (542), Expect = 3.6e-55
Identity = 157/443 (35.44%), Postives = 217/443 (48.98%), Query Frame = 0

Query: 1   MAATSINYFYLLLLFFIFQTTAIRGGGGNGGFTASLFHRDSPLSPLHNPSLTHYHRLNNA 60
           +A T I  F  + L F+F TTA        GFT  L HR S  S           R++N 
Sbjct: 3   LATTIIVLFLQISLCFLFTTTA----SPPHGFTMDLIHRRSNAS----------SRVSNT 62

Query: 61  FRRSFSRAAALFHRAAVEIQSPITPGSGEYIMSVSIGTPPVDFVAIADTGSDLTWTQCLP 120
              S   A  +F  +              Y+M + +GTPP +  AI DTGS++TWTQCLP
Sbjct: 63  QSGSSPYANTVFDNSV-------------YLMKLQVGTPPFEIQAIIDTGSEITWTQCLP 122

Query: 121 CQKCFNQSNPIFNPLQSSSFSHVPCTSKTCHALDVVECGVQGVCDYSYTYGDQTYTKGDL 180
           C  C+ Q+ PIF+P +SS+F    C   +              C Y   Y D TYT G L
Sbjct: 123 CVHCYEQNAPIFDPSKSSTFKEKRCDGHS--------------CPYEVDYFDHTYTMGTL 182

Query: 181 GIDKITFGSSS------VSTVFGCGHESGGGFGVASGVIGLGGGELSLVSQMSQTAAVSR 240
             + IT  S+S        T+ GCGH +       SG++GL  G  SL++QM        
Sbjct: 183 ATETITLHSTSGEPFVMPETIIGCGHNNSWFKPSFSGMVGLNWGPSSLITQMG--GEYPG 242

Query: 241 QFSYCLPTVLSHANGKINFGPNAVVSAPGVVSTPL-VSKIPNTLYYMTLEAVSVGDER-H 300
             SYC          KINFG NA+V+  GVVST + ++      YY+ L+AVSVG+ R  
Sbjct: 243 LMSYCFS---GQGTSKINFGANAIVAGDGVVSTTMFMTTAKPGFYYLNLDAVSVGNTRIE 302

Query: 301 AVDMSAAQAQGNMIIDTGTTLSYLPKELYDGVVSSLVKVVRAKRVEDPGGFFGLCFAADG 360
            +  +    +GN++ID+GTTL+Y P    + V  ++  VV A R  DP G   LC+ +D 
Sbjct: 303 TMGTTFHALEGNIVIDSGTTLTYFPVSYCNLVRQAVEHVVTAVRAADPTGNDMLCYNSD- 362

Query: 361 SGDDGGLDV-PVITAHFAGGANVDFLPVNTFNKVAD-KVSCLTFTPSSPSDPFGILGNLA 420
                 +D+ PVIT HF+GG ++     N + +  +  V CL    +SP+    I GN A
Sbjct: 363 -----TIDIFPVITMHFSGGVDLVLDKYNMYMESNNGGVFCLAIICNSPTQE-AIFGNRA 392

Query: 421 QANFLIGYDLELGKLSFKPTICA 434
           Q NFL+GYD     +SF PT C+
Sbjct: 423 QNNFLVGYDSSSLLVSFSPTNCS 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0044967.17.1e-17070.41putative aspartic protease [Cucumis melo var. makuwa][more]
XP_008452153.11.6e-16970.97PREDICTED: probable aspartic protease At2g35615 [Cucumis melo] >TYK16501.1 putat... [more]
XP_038889229.12.3e-16871.00aspartic proteinase CDR1-like [Benincasa hispida][more]
XP_031739975.16.7e-16869.27probable aspartic protease At2g35615 [Cucumis sativus] >KAE8649216.1 hypothetica... [more]
XP_008452152.13.7e-16669.27PREDICTED: probable aspartic protease At2g35615 [Cucumis melo] >TYK16500.1 putat... [more]
Match NameE-valueIdentityDescription
Q3EBM59.9e-9043.11Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g3561... [more]
Q6XBF82.1e-8744.76Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1[more]
Q766C31.3e-6037.77Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C26.9e-5937.76Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q9LS402.5e-4833.61Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Match NameE-valueIdentityDescription
A0A5A7TSV33.5e-17070.41Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A5D3CX417.7e-17070.97Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3BUB07.7e-17070.97probable aspartic protease At2g35615 OS=Cucumis melo OX=3656 GN=LOC103493257 PE=... [more]
A0A5D3CYR21.8e-16669.27Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3BTZ41.8e-16669.27probable aspartic protease At2g35615 OS=Cucumis melo OX=3656 GN=LOC103493256 PE=... [more]
Match NameE-valueIdentityDescription
AT1G64830.14.9e-9245.08Eukaryotic aspartyl protease family protein [more]
AT2G35615.17.0e-9143.11Eukaryotic aspartyl protease family protein [more]
AT5G33340.11.5e-8844.76Eukaryotic aspartyl protease family protein [more]
AT1G31450.16.4e-8441.58Eukaryotic aspartyl protease family protein [more]
AT2G28010.13.6e-5535.44Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 305..316
score: 47.4
coord: 404..419
score: 25.88
coord: 96..116
score: 45.65
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 253..433
e-value: 2.2E-43
score: 150.0
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 68..252
e-value: 2.0E-51
score: 176.7
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 83..432
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 278..428
e-value: 2.4E-27
score: 95.7
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 90..254
e-value: 1.4E-48
score: 165.4
NoneNo IPR availablePANTHERPTHR47967OS07G0603500 PROTEIN-RELATEDcoord: 12..432
NoneNo IPR availablePANTHERPTHR47967:SF66ASPARTIC PROTEINASE CDR1-RELATEDcoord: 12..432
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 305..316
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 90..428
score: 39.51981
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 89..432
e-value: 1.11463E-84
score: 258.347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0019360.1Lag0019360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005576 extracellular region
molecular_function GO:0004190 aspartic-type endopeptidase activity