Lag0019330 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0019330
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNA polymerase II transcriptional coactivator KELP-like
Locationchr5: 41131527 .. 41132973 (+)
RNA-Seq ExpressionLag0019330
SyntenyLag0019330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCCGAAATCCAACGCAGAATCGAGGAAGCTGTCCGCAGAATTCTCGAATCCTCCGACATGGACCAAATGACCGAGTCCAAGATTCGAGCCCTCGCCTCCAAGGACCTCGATCTCGACCTCTCCAAATCCCCTTACAAGGCTCTCGTTCGCCAGGTTGTCGAGTCCTTCCTCCAAGAACGATCTGAACAACAGCCGCAAGACGACGCCGACGACGCCTCTGCTGCCAAGGAGAAGGAGTACGACGACGACGGCGATCTCGTTGTTTGTTGGGTTTGTTTTTTTTTTTTTTTTTTTGCCTCATCTCCTTTCTAGTAGCTTCCGATTGCGGTTTCTTCTGTTTGTAGTGAATACGTGGCGATTTTATGCTCTGATTCTATGTTTGCGGTGTTCTTTGGCTCAGTTATCGAGCAAGAGGAAGGTGACGATTCAGGATTTTAAAGGGAAGACTCTGGTTTCGATTAGGGAGTTCTTTAGAAAGGATGGGAAAGACCTCCCTACGTCAAAAGGTGTTTGTCTAATGCCTTATTGCTTGTGTTTATGAAATTCTGTATGTTAATGTGTTGTTAATTGCATGTGTTTATGAAACTCTGTATGTTAACATATTACTAATTGCTTGTGTTTATGAAGTTGTGTATGTTAATATATTACTTACAGAGACTGCAAATTAGGCAAAACTCCTATTTTTTTATATGTGCTTCTTGTTCGATACTGTGAAGATATTGAACATTATACTACTCCCTAACCACATCACACTCAGTAATACAATACCCTGATGCATTGAGAACTTTTACTGGGTGAAGCAAATTGACCGGTTCTTAGAACATGTTGGTGAATTAGAAGTAAAAACAATTAAAGGGAATGGGTACGATCCATGATTGCCATTTACCTAAGATTTAATATCATATCCTACATGTTTCCTGACAACCAAATGTAGTAGAGTCAAACGGTTGTTCCGTGAGATTGGTCGAGGTGCGTGCAAGCGGACACAAACATTCACGGATATAAAAAAAATATATAAATAAAAATAACACAATTATTATTGGACCATTGACGTGTGGGAAATGTGGGAAGATAATATTCATTGAAATATAGGCTAGAACCCTTTATTAAAGTTAAATGAGCAACTGCAGCCACACATTTGATTTGTGATGTATCTGATACAAGGTAATGCGTGTAATTTGAATAAAGCAATGAACAGGGGTGATGTTTATAAAACCACTGCATGTTGATCTCTGTTCTCATGAACTTGTTCTTTGATCAAACTTATATAATTTTTGTGTGTTGGAAATTTGAATGGCTAGAGTAGTTATTTCAGACCAATAACATTGGTTTTGTTTGTTTCTCAGGAATAAGTTTGACCGAAGAACAATGGTCTGTCTTTAAAAAGAATGTACCTGCCATAGAAAAAGCTATTAAGAATATGCAATCACAAGCAATGTGA

mRNA sequence

ATGGATTCCGAAATCCAACGCAGAATCGAGGAAGCTGTCCGCAGAATTCTCGAATCCTCCGACATGGACCAAATGACCGAGTCCAAGATTCGAGCCCTCGCCTCCAAGGACCTCGATCTCGACCTCTCCAAATCCCCTTACAAGGCTCTCGTTCGCCAGGTTGTCGAGTCCTTCCTCCAAGAACGATCTGAACAACAGCCGCAAGACGACGCCGACGACGCCTCTGCTGCCAAGGAGAAGGAGTACGACGACGACGGCGATCTCGTTGTTTGTTGGTTATCGAGCAAGAGGAAGGTGACGATTCAGGATTTTAAAGGGAAGACTCTGGTTTCGATTAGGGAGTTCTTTAGAAAGGATGGGAAAGACCTCCCTACGTCAAAAGGAATAAGTTTGACCGAAGAACAATGGTCTGTCTTTAAAAAGAATGTACCTGCCATAGAAAAAGCTATTAAGAATATGCAATCACAAGCAATGTGA

Coding sequence (CDS)

ATGGATTCCGAAATCCAACGCAGAATCGAGGAAGCTGTCCGCAGAATTCTCGAATCCTCCGACATGGACCAAATGACCGAGTCCAAGATTCGAGCCCTCGCCTCCAAGGACCTCGATCTCGACCTCTCCAAATCCCCTTACAAGGCTCTCGTTCGCCAGGTTGTCGAGTCCTTCCTCCAAGAACGATCTGAACAACAGCCGCAAGACGACGCCGACGACGCCTCTGCTGCCAAGGAGAAGGAGTACGACGACGACGGCGATCTCGTTGTTTGTTGGTTATCGAGCAAGAGGAAGGTGACGATTCAGGATTTTAAAGGGAAGACTCTGGTTTCGATTAGGGAGTTCTTTAGAAAGGATGGGAAAGACCTCCCTACGTCAAAAGGAATAAGTTTGACCGAAGAACAATGGTCTGTCTTTAAAAAGAATGTACCTGCCATAGAAAAAGCTATTAAGAATATGCAATCACAAGCAATGTGA

Protein sequence

MDSEIQRRIEEAVRRILESSDMDQMTESKIRALASKDLDLDLSKSPYKALVRQVVESFLQERSEQQPQDDADDASAAKEKEYDDDGDLVVCWLSSKRKVTIQDFKGKTLVSIREFFRKDGKDLPTSKGISLTEEQWSVFKKNVPAIEKAIKNMQSQAM
Homology
[Close]
BLAST of Lag0019330 vs. NCBI nr
Match: XP_038878439.1 (RNA polymerase II transcriptional coactivator KELP-like [Benincasa hispida])

HSP 1 Score: 282.0 bits (720), Expect = 3.3e-72
Identity = 147/156 (94.23%), Postives = 155/156 (99.36%), Query Frame = 0 

Query: 1   MDSEIQRRIEEAVRRILESSDMDQMTESKIRALASKDLDLDLSKSPYKALVRQVVESFLQ 60
           MDSEIQRRIEEAVRRILESSDMD+MTESKIRALASKDLDLDLSKSPYKALVR+VVESFLQ
Sbjct: 1   MDSEIQRRIEEAVRRILESSDMDEMTESKIRALASKDLDLDLSKSPYKALVRKVVESFLQ 60

Query: 61  ERSEQQPQDDADDASAAKEKEYDDDGDLVVCWLSSKRKVTIQDFKGKTLVSIREFFRKDG 120
           ERSE+QPQD ADDASAAKEKEYDDDGDL+VCWLSSKRKVTIQDF+GKTLVSIREF+RKDG
Sbjct: 61  ERSEEQPQDQADDASAAKEKEYDDDGDLIVCWLSSKRKVTIQDFRGKTLVSIREFYRKDG 120

Query: 121 KDLPTSKGISLTEEQWSVFKKNVPAIEKAIKNMQSQ 157
           KDLPT+KGISLTEEQWSVFKKNVPAIEKAIKNM+SQ
Sbjct: 121 KDLPTAKGISLTEEQWSVFKKNVPAIEKAIKNMRSQ 156

[Close]
BLAST of Lag0019330 vs. NCBI nr
Match: XP_022931551.1 (RNA polymerase II transcriptional coactivator KELP-like [Cucurbita moschata] >XP_023551821.1 RNA polymerase II transcriptional coactivator KELP-like [Cucurbita pepo subsp. pepo] >KAG6577134.1 RNA polymerase II transcriptional coactivator KELP, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015130.1 RNA polymerase II transcriptional coactivator KELP [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 280.0 bits (715), Expect = 1.2e-71
Identity = 146/158 (92.41%), Postives = 155/158 (98.10%), Query Frame = 0 

Query: 1   MDSEIQRRIEEAVRRILESSDMDQMTESKIRALASKDLDLDLSKSPYKALVRQVVESFLQ 60
           MDSEIQRRIEEAVRRILESSDMD+MTESKIRALASKDLDLDLSKSPYKALVR+VVESFLQ
Sbjct: 1   MDSEIQRRIEEAVRRILESSDMDEMTESKIRALASKDLDLDLSKSPYKALVRKVVESFLQ 60

Query: 61  ERSEQQPQDDADDASAAKEKEYDDDGDLVVCWLSSKRKVTIQDFKGKTLVSIREFFRKDG 120
           ERSE QPQ++A+DASAAKEKEYDDDGDL+VCWLSSKRKVTIQDF+GKTLVSIREF+RKDG
Sbjct: 61  ERSEDQPQEEAEDASAAKEKEYDDDGDLIVCWLSSKRKVTIQDFRGKTLVSIREFYRKDG 120

Query: 121 KDLPTSKGISLTEEQWSVFKKNVPAIEKAIKNMQSQAM 159
           KDLPT+KGISLTEEQWSVFK NVPAIEKAIKNMQSQ M
Sbjct: 121 KDLPTAKGISLTEEQWSVFKNNVPAIEKAIKNMQSQIM 158

[Close]
BLAST of Lag0019330 vs. NCBI nr
Match: XP_008451928.1 (PREDICTED: RNA polymerase II transcriptional coactivator KELP [Cucumis melo] >KAA0044928.1 RNA polymerase II transcriptional coactivator KELP [Cucumis melo var. makuwa] >TYK16542.1 RNA polymerase II transcriptional coactivator KELP [Cucumis melo var. makuwa])

HSP 1 Score: 279.6 bits (714), Expect = 1.6e-71
Identity = 146/158 (92.41%), Postives = 154/158 (97.47%), Query Frame = 0 

Query: 1   MDSEIQRRIEEAVRRILESSDMDQMTESKIRALASKDLDLDLSKSPYKALVRQVVESFLQ 60
           MDSE QRRIEEAVRRILE+SDMD+MTESKIRALASKDLDLDLSKSPYK+LVR VVESFLQ
Sbjct: 1   MDSETQRRIEEAVRRILETSDMDEMTESKIRALASKDLDLDLSKSPYKSLVRTVVESFLQ 60

Query: 61  ERSEQQPQDDADDASAAKEKEYDDDGDLVVCWLSSKRKVTIQDFKGKTLVSIREFFRKDG 120
           ERSEQQPQD A+DASAAKEKEYDDDGDL+VCWLSSKRKVTIQDF+GKTLVSIREF+RKDG
Sbjct: 61  ERSEQQPQDQAEDASAAKEKEYDDDGDLIVCWLSSKRKVTIQDFRGKTLVSIREFYRKDG 120

Query: 121 KDLPTSKGISLTEEQWSVFKKNVPAIEKAIKNMQSQAM 159
           KDLPT+KGISLTEEQWSVFKKNVPAIEKAIKNMQSQ M
Sbjct: 121 KDLPTAKGISLTEEQWSVFKKNVPAIEKAIKNMQSQIM 158

[Close]
BLAST of Lag0019330 vs. NCBI nr
Match: XP_022985429.1 (RNA polymerase II transcriptional coactivator KELP-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 273.1 bits (697), Expect = 1.5e-69
Identity = 143/158 (90.51%), Postives = 154/158 (97.47%), Query Frame = 0 

Query: 1   MDSEIQRRIEEAVRRILESSDMDQMTESKIRALASKDLDLDLSKSPYKALVRQVVESFLQ 60
           MDS+IQRRIEEAVRRILESSDMD+MTESKIRALASKDLDLDLSKSPYKALVR+VVESFLQ
Sbjct: 1   MDSKIQRRIEEAVRRILESSDMDEMTESKIRALASKDLDLDLSKSPYKALVRKVVESFLQ 60

Query: 61  ERSEQQPQDDADDASAAKEKEYDDDGDLVVCWLSSKRKVTIQDFKGKTLVSIREFFRKDG 120
           +RSE Q Q++A+DASAAKEKEYDDDGDL+VCWLSSKRKVTIQDF+GKTLVSIREF+RKDG
Sbjct: 61  KRSEDQLQEEAEDASAAKEKEYDDDGDLIVCWLSSKRKVTIQDFRGKTLVSIREFYRKDG 120

Query: 121 KDLPTSKGISLTEEQWSVFKKNVPAIEKAIKNMQSQAM 159
           KDLPT+KGISLTEEQWSVFK NVPAIEKAIKNMQSQ M
Sbjct: 121 KDLPTAKGISLTEEQWSVFKNNVPAIEKAIKNMQSQIM 158

[Close]
BLAST of Lag0019330 vs. NCBI nr
Match: XP_004148989.1 (RNA polymerase II transcriptional coactivator KELP [Cucumis sativus] >KGN53421.1 hypothetical protein Csa_014494 [Cucumis sativus])

HSP 1 Score: 270.8 bits (691), Expect = 7.5e-69
Identity = 141/158 (89.24%), Postives = 151/158 (95.57%), Query Frame = 0 

Query: 1   MDSEIQRRIEEAVRRILESSDMDQMTESKIRALASKDLDLDLSKSPYKALVRQVVESFLQ 60
           MDS++QRRIEEAVR ILESSDMD+MTESKIRALASKDL LDLSKSPYK+LVR VVESFLQ
Sbjct: 1   MDSQLQRRIEEAVRAILESSDMDEMTESKIRALASKDLHLDLSKSPYKSLVRTVVESFLQ 60

Query: 61  ERSEQQPQDDADDASAAKEKEYDDDGDLVVCWLSSKRKVTIQDFKGKTLVSIREFFRKDG 120
           ERSEQ P+D  +DASAAKEKEYDDDGDL+VCWLSSKRKVTIQDF+GKTLVSIREF+RKDG
Sbjct: 61  ERSEQHPEDQPEDASAAKEKEYDDDGDLIVCWLSSKRKVTIQDFRGKTLVSIREFYRKDG 120

Query: 121 KDLPTSKGISLTEEQWSVFKKNVPAIEKAIKNMQSQAM 159
           KDLPT+KGISLTEEQWSVFKKNVPAIEKAIKNMQSQ M
Sbjct: 121 KDLPTAKGISLTEEQWSVFKKNVPAIEKAIKNMQSQIM 158

[Close]
BLAST of Lag0019330 vs. ExPASy Swiss-Prot
Match: O65155 (RNA polymerase II transcriptional coactivator KELP OS=Arabidopsis thaliana OX=3702 GN=KELP PE=1 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 1.1e-41
Identity = 90/164 (54.88%), Postives = 120/164 (73.17%), Query Frame = 0 

Query: 1   MDSEIQRRIEEAVRRILESSDMDQMTESKIRALASKDLDLDLSKSPYKALVRQVVESFLQ 60
           M+ E + +IE+ V  IL  SDM ++TE K+R LAS+ L +DLS+  +KA VR VVE FL 
Sbjct: 1   MEKETKEKIEKTVIEILSESDMKEITEFKVRKLASEKLAIDLSEKSHKAFVRSVVEKFLD 60

Query: 61  ERSEQQ--------PQDDADDASAAKEKEYDDDGDLVVCWLSSKRKVTIQDFKGKTLVSI 120
           E   ++         ++D D       KE+DDDGDL++C LS KR+VTIQ+FKGK+LVSI
Sbjct: 61  EERAREYENSQVNKEEEDGDKDCGKGNKEFDDDGDLIICRLSDKRRVTIQEFKGKSLVSI 120

Query: 121 REFFRKDGKDLPTSKGISLTEEQWSVFKKNVPAIEKAIKNMQSQ 157
           RE+++KDGK+LPTSKGISLT+EQWS FKKN+PAIE A+K M+S+
Sbjct: 121 REYYKKDGKELPTSKGISLTDEQWSTFKKNMPAIENAVKKMESR 164

[Close]
BLAST of Lag0019330 vs. ExPASy Swiss-Prot
Match: O65154 (RNA polymerase II transcriptional coactivator KIWI OS=Arabidopsis thaliana OX=3702 GN=KIWI PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.3e-11
Identity = 35/91 (38.46%), Postives = 56/91 (61.54%), Query Frame = 0 

Query: 69  DDADDASAAKEK------EYDDDGDLVVCWLSSKRKVTIQDFKGKTLVSIREFFRKDGKD 128
           DD  +  A  +K      + D   D+VVC +S  R+V+++++ GK  + IREF+ KDGK 
Sbjct: 16  DDESETHAPAKKVAKPADDSDQSDDIVVCNISKNRRVSVRNWNGKIWIDIREFYVKDGKT 75

Query: 129 LPTSKGISLTEEQWSVFKKNVPAIEKAIKNM 154
           LP  KGISL+ +QW+  + +   IEKA+ ++
Sbjct: 76  LPGKKGISLSVDQWNTLRNHAEDIEKALSDL 106

[Close]
BLAST of Lag0019330 vs. ExPASy Swiss-Prot
Match: Q9FHX8 (Mediator-associated protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g42060 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.0e-08
Identity = 30/61 (49.18%), Postives = 49/61 (80.33%), Query Frame = 0 

Query: 1  MDSEIQRRIEEAVRRILESSDMDQMTESKIRALASKDLDLDLSKSPYKALVRQVVESFLQ 60
          +D +++R+I++ V++ILESS++ ++TE K R  AS  LDLDLS+ PYK +V++ VE+FL+
Sbjct: 11 IDKDLRRKIKKTVKKILESSNLYKITEIKAREEASLKLDLDLSQDPYKVIVKEEVENFLE 70

Query: 61 E 62
          E
Sbjct: 71 E 71

[Close]
BLAST of Lag0019330 vs. ExPASy Swiss-Prot
Match: Q63396 (Activated RNA polymerase II transcriptional coactivator p15 OS=Rattus norvegicus OX=10116 GN=Sub1 PE=1 SV=3)

HSP 1 Score: 50.8 bits (120), Expect = 1.6e-05
Identity = 29/91 (31.87%), Postives = 54/91 (59.34%), Query Frame = 0 

Query: 64  EQQPQDDADDASAAKEKEYDDDGDLVVCWLSSKRKVTIQDFKGKTLVSIREFFR-KDGKD 123
           +Q+P + +   +++K+     D ++    +   R V+++DFKGK L+ IRE++   +G+ 
Sbjct: 39  KQKPGESSRALASSKQSSSSRDDNMF--QIGKMRYVSVRDFKGKILIDIREYWMDSEGEM 98

Query: 124 LPTSKGISLTEEQWSVFKKNVPAIEKAIKNM 154
            P  KGISL  EQWS  K+ +  I+ A++ +
Sbjct: 99  KPGRKGISLNMEQWSQLKEQISDIDDAVRKL 127

[Close]
BLAST of Lag0019330 vs. ExPASy Swiss-Prot
Match: P11031 (Activated RNA polymerase II transcriptional coactivator p15 OS=Mus musculus OX=10090 GN=Sub1 PE=1 SV=3)

HSP 1 Score: 50.4 bits (119), Expect = 2.1e-05
Identity = 29/91 (31.87%), Postives = 54/91 (59.34%), Query Frame = 0 

Query: 64  EQQPQDDADDASAAKEKEYDDDGDLVVCWLSSKRKVTIQDFKGKTLVSIREFFR-KDGKD 123
           +Q+P + +   +++K+     D ++    +   R V+++DFKGK L+ IRE++   +G+ 
Sbjct: 39  KQKPGETSRALASSKQSSSSRDDNMF--QIGKMRYVSVRDFKGKILIDIREYWMDSEGEM 98

Query: 124 LPTSKGISLTEEQWSVFKKNVPAIEKAIKNM 154
            P  KGISL  EQWS  K+ +  I+ A++ +
Sbjct: 99  KPGRKGISLNMEQWSQLKEQISDIDDAVRKL 127

The following BLAST results are available for this feature:
BLAST of Lag0019330 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038878439.13.3e-7294.23RNA polymerase II transcriptional coactivator KELP-like [Benincasa hispida][more]
XP_022931551.11.2e-7192.41RNA polymerase II transcriptional coactivator KELP-like [Cucurbita moschata] >XP... [more]
XP_008451928.11.6e-7192.41PREDICTED: RNA polymerase II transcriptional coactivator KELP [Cucumis melo] >KA... [more]
XP_022985429.11.5e-6990.51RNA polymerase II transcriptional coactivator KELP-like isoform X3 [Cucurbita ma... [more]
XP_004148989.17.5e-6989.24RNA polymerase II transcriptional coactivator KELP [Cucumis sativus] >KGN53421.1... [more]
BLAST of Lag0019330 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O651551.1e-4154.88RNA polymerase II transcriptional coactivator KELP OS=Arabidopsis thaliana OX=37... [more]
O651543.3e-1138.46RNA polymerase II transcriptional coactivator KIWI OS=Arabidopsis thaliana OX=37... [more]
Q9FHX82.0e-0849.18Mediator-associated protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g42060 PE=1 ... [more]
Q633961.6e-0531.87Activated RNA polymerase II transcriptional coactivator p15 OS=Rattus norvegicus... [more]
P110312.1e-0531.87Activated RNA polymerase II transcriptional coactivator p15 OS=Mus musculus OX=1... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017415Putative RNA polymerase II transcriptional coactivator KELPPIRSFPIRSF038156RNA_polymII_KELPcoord: 1..157
e-value: 1.9E-54
score: 181.9
IPR003173Transcriptional coactivator p15 (PC4), C-terminalPFAMPF02229PC4coord: 93..141
e-value: 9.7E-21
score: 73.2
IPR014876DEK, C-terminalPFAMPF08766DEK_Ccoord: 8..59
e-value: 4.2E-12
score: 45.8
IPR009044ssDNA-binding transcriptional regulatorGENE3D2.30.31.10Transcriptional Coactivator Pc4; Chain Acoord: 79..157
e-value: 2.6E-26
score: 93.0
IPR009044ssDNA-binding transcriptional regulatorSUPERFAMILY54447ssDNA-binding transcriptional regulator domaincoord: 90..152
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 60..83
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 63..83
NoneNo IPR availablePANTHERPTHR13215:SF6RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR KELPcoord: 3..156
IPR045125RNA polymerase II transcriptional coactivator Sub1/Tcp4-likePANTHERPTHR13215RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATORcoord: 3..156

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0019330.1Lag0019330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0060261 positive regulation of transcription initiation from RNA polymerase II promoter
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
cellular_component GO:0005667 transcription regulator complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0003713 transcription coactivator activity