Lag0019274 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0019274
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionDirigent protein
Locationchr5: 40481387 .. 40481950 (-)
RNA-Seq ExpressionLag0019274
SyntenyLag0019274
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTCTCAGCAAAAGTTTGCATTCTTCTAGTTTTGTTCTTGTTCTCTTCTTCTTCCTCCTCAGCCTTCCCAGGAAAGAAGCAAAAACCCAAGCCTTGTAAAGAGCTAGTTCTTTACTTTCATGACATTATTTACAATGGAACGAATGCAGCCAATGCCACTTCGGCCATTGTTGCAGCCCCTGAAGGTTCAAACTTGACAATTTTGGCACCCCACAACCACTTTGGAAACATAGTCGTTTTCGACGATCCCCTTACCTTGGACAACAATCTCCACTCGCCTCCCGTGGGTCGAGCTCAAGGGCTTTACCTTTACGACACCAAAAATACCTTCACCGCATGGTTCGGCTTCACGTTTTCTCTCAACTACACGTCCTATGTTGGCACCATCAACTGCATCGGAGCTGACCCGGCCTTGGTGAAGACTAGAGACATCTCCGTCGTCGGTGGCACCGGAGACTTCTTCATGCACCGCGGTATTGCTACCATCATGACTGATGCCGTTGAAGATGATGTCTACTTTCGACTCCGATTTGATATCAAGTTCTACGAGTGCTGGTGA

mRNA sequence

ATGGCTTTCTCAGCAAAAGTTTGCATTCTTCTAGTTTTGTTCTTGTTCTCTTCTTCTTCCTCCTCAGCCTTCCCAGGAAAGAAGCAAAAACCCAAGCCTTGTAAAGAGCTAGTTCTTTACTTTCATGACATTATTTACAATGGAACGAATGCAGCCAATGCCACTTCGGCCATTGTTGCAGCCCCTGAAGGTTCAAACTTGACAATTTTGGCACCCCACAACCACTTTGGAAACATAGTCGTTTTCGACGATCCCCTTACCTTGGACAACAATCTCCACTCGCCTCCCGTGGGTCGAGCTCAAGGGCTTTACCTTTACGACACCAAAAATACCTTCACCGCATGGTTCGGCTTCACGTTTTCTCTCAACTACACGTCCTATGTTGGCACCATCAACTGCATCGGAGCTGACCCGGCCTTGGTGAAGACTAGAGACATCTCCGTCGTCGGTGGCACCGGAGACTTCTTCATGCACCGCGGTATTGCTACCATCATGACTGATGCCGTTGAAGATGATGTCTACTTTCGACTCCGATTTGATATCAAGTTCTACGAGTGCTGGTGA

Coding sequence (CDS)

ATGGCTTTCTCAGCAAAAGTTTGCATTCTTCTAGTTTTGTTCTTGTTCTCTTCTTCTTCCTCCTCAGCCTTCCCAGGAAAGAAGCAAAAACCCAAGCCTTGTAAAGAGCTAGTTCTTTACTTTCATGACATTATTTACAATGGAACGAATGCAGCCAATGCCACTTCGGCCATTGTTGCAGCCCCTGAAGGTTCAAACTTGACAATTTTGGCACCCCACAACCACTTTGGAAACATAGTCGTTTTCGACGATCCCCTTACCTTGGACAACAATCTCCACTCGCCTCCCGTGGGTCGAGCTCAAGGGCTTTACCTTTACGACACCAAAAATACCTTCACCGCATGGTTCGGCTTCACGTTTTCTCTCAACTACACGTCCTATGTTGGCACCATCAACTGCATCGGAGCTGACCCGGCCTTGGTGAAGACTAGAGACATCTCCGTCGTCGGTGGCACCGGAGACTTCTTCATGCACCGCGGTATTGCTACCATCATGACTGATGCCGTTGAAGATGATGTCTACTTTCGACTCCGATTTGATATCAAGTTCTACGAGTGCTGGTGA

Protein sequence

MAFSAKVCILLVLFLFSSSSSSAFPGKKQKPKPCKELVLYFHDIIYNGTNAANATSAIVAAPEGSNLTILAPHNHFGNIVVFDDPLTLDNNLHSPPVGRAQGLYLYDTKNTFTAWFGFTFSLNYTSYVGTINCIGADPALVKTRDISVVGGTGDFFMHRGIATIMTDAVEDDVYFRLRFDIKFYECW
Homology
BLAST of Lag0019274 vs. NCBI nr
Match: KAG6577167.1 (Disease resistance response protein 206, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 323.2 bits (827), Expect = 1.5e-84
Identity = 159/189 (84.13%), Postives = 170/189 (89.95%), Query Frame = 0

Query: 1   MAFSAKVCILLV-LFLFSSSSSSAFP-GKKQKPKPCKELVLYFHDIIYNGTNAANATSAI 60
           MA SAK CI+LV LFLFS SSSSAFP GKKQ+PKPCKELVLYFHDIIYNG+NA NATSAI
Sbjct: 1   MASSAKNCIILVALFLFSISSSSAFPMGKKQRPKPCKELVLYFHDIIYNGSNAGNATSAI 60

Query: 61  VAAPEGSNLTILAPHNHFGNIVVFDDPLTLDNNLHSPPVGRAQGLYLYDTKNTFTAWFGF 120
           VAAP G+NLTILAP  HFGNI VFDDP+TLDNNLHS PVGRAQGLY+YDTKNTFTAW GF
Sbjct: 61  VAAPHGANLTILAPQFHFGNIAVFDDPITLDNNLHSKPVGRAQGLYIYDTKNTFTAWLGF 120

Query: 121 TFSLNYTSYVGTINCIGADPALVKTRDISVVGGTGDFFMHRGIATIMTDAVEDDVYFRLR 180
           +FSLNYT+Y GTIN IGADP LVK RDISVVGGTGDFFMHRG+ATI+TDA E +VYFRLR
Sbjct: 121 SFSLNYTAYKGTINFIGADPILVKARDISVVGGTGDFFMHRGVATIVTDAFEGEVYFRLR 180

Query: 181 FDIKFYECW 188
            DIKFYECW
Sbjct: 181 VDIKFYECW 189

BLAST of Lag0019274 vs. NCBI nr
Match: XP_022931436.1 (disease resistance response protein 206-like [Cucurbita moschata])

HSP 1 Score: 322.8 bits (826), Expect = 2.0e-84
Identity = 159/189 (84.13%), Postives = 169/189 (89.42%), Query Frame = 0

Query: 1   MAFSAKVCILLV-LFLFSSSSSSAFP-GKKQKPKPCKELVLYFHDIIYNGTNAANATSAI 60
           MA SAK CI+LV LFLFS SSSSAFP GKKQ+PKPCKELVLYFHDIIYNG NA NATSAI
Sbjct: 1   MASSAKNCIILVALFLFSISSSSAFPMGKKQRPKPCKELVLYFHDIIYNGNNAGNATSAI 60

Query: 61  VAAPEGSNLTILAPHNHFGNIVVFDDPLTLDNNLHSPPVGRAQGLYLYDTKNTFTAWFGF 120
           VAAP G+NLTILAP  HFGNI VFDDP+TLDNNLHS PVGRAQGLY+YDTKNTFTAW GF
Sbjct: 61  VAAPHGANLTILAPQFHFGNIAVFDDPITLDNNLHSKPVGRAQGLYIYDTKNTFTAWLGF 120

Query: 121 TFSLNYTSYVGTINCIGADPALVKTRDISVVGGTGDFFMHRGIATIMTDAVEDDVYFRLR 180
           +FSLNYT+Y GTIN IGADP LVK RDISVVGGTGDFFMHRG+ATI+TDA E +VYFRLR
Sbjct: 121 SFSLNYTAYKGTINFIGADPILVKARDISVVGGTGDFFMHRGVATIVTDAFEGEVYFRLR 180

Query: 181 FDIKFYECW 188
            DIKFYECW
Sbjct: 181 VDIKFYECW 189

BLAST of Lag0019274 vs. NCBI nr
Match: XP_022985425.1 (disease resistance response protein 206-like [Cucurbita maxima])

HSP 1 Score: 322.8 bits (826), Expect = 2.0e-84
Identity = 160/189 (84.66%), Postives = 168/189 (88.89%), Query Frame = 0

Query: 1   MAFSAKVC-ILLVLFLFSSSSSSAFP-GKKQKPKPCKELVLYFHDIIYNGTNAANATSAI 60
           MA SAK C ILL LFLFS SSSSAFP GKKQKPKPCKELVLYFHDIIYNG NA NATSAI
Sbjct: 1   MASSAKNCIILLALFLFSISSSSAFPWGKKQKPKPCKELVLYFHDIIYNGKNAGNATSAI 60

Query: 61  VAAPEGSNLTILAPHNHFGNIVVFDDPLTLDNNLHSPPVGRAQGLYLYDTKNTFTAWFGF 120
           VAAP G+NLTILAP  HFGNI VFDDP+TLDNNLHS PVGRAQGLY+YDTKNTFTAW GF
Sbjct: 61  VAAPHGANLTILAPQFHFGNIAVFDDPITLDNNLHSKPVGRAQGLYIYDTKNTFTAWLGF 120

Query: 121 TFSLNYTSYVGTINCIGADPALVKTRDISVVGGTGDFFMHRGIATIMTDAVEDDVYFRLR 180
           +FSLNYT+Y GTIN IGADP LVK RDISVVGGTGDFFMHRG+ATI+TDA E +VYFRLR
Sbjct: 121 SFSLNYTAYKGTINFIGADPILVKARDISVVGGTGDFFMHRGVATIVTDAFEGEVYFRLR 180

Query: 181 FDIKFYECW 188
            DIKFYECW
Sbjct: 181 VDIKFYECW 189

BLAST of Lag0019274 vs. NCBI nr
Match: XP_023553111.1 (disease resistance response protein 206-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 318.9 bits (816), Expect = 2.9e-83
Identity = 157/189 (83.07%), Postives = 168/189 (88.89%), Query Frame = 0

Query: 1   MAFSAKVCI-LLVLFLFSSSSSSAFP-GKKQKPKPCKELVLYFHDIIYNGTNAANATSAI 60
           MA +A+ CI LL LFLFS SSSSAFP GKKQ+PKPCKELVLYFHDIIYNG NA NATSAI
Sbjct: 1   MASTARNCIMLLALFLFSISSSSAFPWGKKQRPKPCKELVLYFHDIIYNGKNARNATSAI 60

Query: 61  VAAPEGSNLTILAPHNHFGNIVVFDDPLTLDNNLHSPPVGRAQGLYLYDTKNTFTAWFGF 120
           VAAP G+NLTILAP  HFGNI VFDDP+TLDNNLHS PVGRAQGLY+YDTKNTFTAW GF
Sbjct: 61  VAAPHGANLTILAPQFHFGNIAVFDDPITLDNNLHSKPVGRAQGLYIYDTKNTFTAWLGF 120

Query: 121 TFSLNYTSYVGTINCIGADPALVKTRDISVVGGTGDFFMHRGIATIMTDAVEDDVYFRLR 180
           +FSLNYT+Y GTIN IGADP LVK RDISVVGGTGDFFMHRG+ATI+TDA E +VYFRLR
Sbjct: 121 SFSLNYTAYKGTINFIGADPILVKARDISVVGGTGDFFMHRGVATIVTDAFEGEVYFRLR 180

Query: 181 FDIKFYECW 188
            DIKFYECW
Sbjct: 181 VDIKFYECW 189

BLAST of Lag0019274 vs. NCBI nr
Match: XP_038906438.1 (disease resistance response protein 206-like [Benincasa hispida])

HSP 1 Score: 318.5 bits (815), Expect = 3.7e-83
Identity = 156/189 (82.54%), Postives = 169/189 (89.42%), Query Frame = 0

Query: 1   MAFSAKVCILLV-LFLFSSSSSSAFP-GKKQKPKPCKELVLYFHDIIYNGTNAANATSAI 60
           MAFS + CI+L+ L LFS+SSSSA P GKKQK KPCKELVLYFHDIIYNG NA NATSAI
Sbjct: 1   MAFSTRNCIILIALLLFSTSSSSASPWGKKQKHKPCKELVLYFHDIIYNGKNAGNATSAI 60

Query: 61  VAAPEGSNLTILAPHNHFGNIVVFDDPLTLDNNLHSPPVGRAQGLYLYDTKNTFTAWFGF 120
           VAAPEG+NLTILAP  HFGNIVVFDDP+TLDNNLHS PVGRAQGLY+YDTKNTFT+W  F
Sbjct: 61  VAAPEGANLTILAPQFHFGNIVVFDDPITLDNNLHSNPVGRAQGLYIYDTKNTFTSWLAF 120

Query: 121 TFSLNYTSYVGTINCIGADPALVKTRDISVVGGTGDFFMHRGIATIMTDAVEDDVYFRLR 180
           +FSLNYT+Y GTIN +GADP LVKTRDISVVGGTGDFFMHRG+ATIMTDA E +VYFRLR
Sbjct: 121 SFSLNYTAYKGTINFVGADPILVKTRDISVVGGTGDFFMHRGVATIMTDAFEGEVYFRLR 180

Query: 181 FDIKFYECW 188
            DIKFYECW
Sbjct: 181 VDIKFYECW 189

BLAST of Lag0019274 vs. ExPASy Swiss-Prot
Match: P13240 (Disease resistance response protein 206 OS=Pisum sativum OX=3888 GN=PI206 PE=1 SV=2)

HSP 1 Score: 251.9 bits (642), Expect = 5.6e-66
Identity = 123/180 (68.33%), Postives = 141/180 (78.33%), Query Frame = 0

Query: 10  LLVLFLFSS--SSSSAFPGKKQKPKPCKELVLYFHDIIYNGTNAANATSAIVAAPEGSNL 69
           LLVLF+F    + SSA P K++  KPCK LV YFHDI+YNG NAANATSAIVAAPEG +L
Sbjct: 5   LLVLFVFVMLFALSSAIPNKRKPYKPCKNLVFYFHDILYNGKNAANATSAIVAAPEGVSL 64

Query: 70  TILAPHNHFGNIVVFDDPLTLDNNLHSPPVGRAQGLYLYDTKNTFTAWFGFTFSLNYTSY 129
           T LAP +HFGNI+VFDDP+TL ++L S  VGRAQG Y+YDTKNT+T+W  FTF LN T +
Sbjct: 65  TKLAPQSHFGNIIVFDDPITLSHSLSSKQVGRAQGFYIYDTKNTYTSWLSFTFVLNSTHH 124

Query: 130 VGTINCIGADPALVKTRDISVVGGTGDFFMHRGIATIMTDAVEDDVYFRLRFDIKFYECW 188
            GTI   GADP + KTRDISV GGTGDFFMHRGIATI TDA E + YFRL   IKF+ECW
Sbjct: 125 QGTITFAGADPIVAKTRDISVTGGTGDFFMHRGIATITTDAFEGEAYFRLGVYIKFFECW 184

BLAST of Lag0019274 vs. ExPASy Swiss-Prot
Match: Q1ZZU9 (Dirigent protein OS=Sinopodophyllum hexandrum OX=93608 GN=DIR PE=2 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 3.6e-65
Identity = 118/188 (62.77%), Postives = 148/188 (78.72%), Query Frame = 0

Query: 2   AFSAKVCILLVLFLFSSSSSSAFPGKKQKPK--PCKELVLYFHDIIYNGTNAANATSAIV 61
           AFS    + L  FL + S+SSA P +++  +  PCK+LVLYFHD++YNG N ANAT++IV
Sbjct: 6   AFSFIFLLFLCFFLANLSASSAHPPRQKLKQRIPCKQLVLYFHDVVYNGHNKANATASIV 65

Query: 62  AAPEGSNLTILAPHNHFGNIVVFDDPLTLDNNLHSPPVGRAQGLYLYDTKNTFTAWFGFT 121
            AP+G++L  LA  NHFGN+VVFDDP+TLDNN HSPPVGRAQGLY+YD K+TF +W  F+
Sbjct: 66  GAPQGADLVKLAGENHFGNVVVFDDPITLDNNFHSPPVGRAQGLYVYDKKDTFHSWLSFS 125

Query: 122 FSLNYTSYVGTINCIGADPALVKTRDISVVGGTGDFFMHRGIATIMTDAVEDDVYFRLRF 181
           F+LN T + GT+  +GADP L+K RDI+VVGGTGDFFM RGIATI TD+ E +VYFRL+ 
Sbjct: 126 FTLNTTMHQGTLIFMGADPILIKNRDITVVGGTGDFFMARGIATIATDSYEGEVYFRLKV 185

Query: 182 DIKFYECW 188
           DIK YECW
Sbjct: 186 DIKLYECW 193

BLAST of Lag0019274 vs. ExPASy Swiss-Prot
Match: Q9SH66 (Dirigent protein 5 OS=Arabidopsis thaliana OX=3702 GN=DIR5 PE=2 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 1.9e-45
Identity = 88/178 (49.44%), Postives = 113/178 (63.48%), Query Frame = 0

Query: 10  LLVLFLFSSSSSSAFPGKKQKPKPCKELVLYFHDIIYNGTNAANATSAIVAAPEGSNLTI 69
           L V  + + +  SA    K +PKPCK  VLY+HDI++   +  NATSA V  P G     
Sbjct: 11  LFVFLVLTKTVISARKPSKSQPKPCKNFVLYYHDIMFGVDDVQNATSAAVTNPPGLG--- 70

Query: 70  LAPHNHFGNIVVFDDPLTLDNNLHSPPVGRAQGLYLYDTKNTFTAWFGFTFSLNYTSYVG 129
              +  FG +V+FDDP+T+D N  S PV RAQG Y YD KN + AWF +T   N T + G
Sbjct: 71  ---NFKFGKLVIFDDPMTIDKNFQSEPVARAQGFYFYDMKNDYNAWFAYTLVFNSTQHKG 130

Query: 130 TINCIGADPALVKTRDISVVGGTGDFFMHRGIATIMTDAVEDDVYFRLRFDIKFYECW 188
           T+N +GAD  +V++RD+SVVGGTGDFFM RGI T  TD  E   YFR++ DIK YEC+
Sbjct: 131 TLNIMGADLMMVQSRDLSVVGGTGDFFMSRGIVTFETDTFEGAKYFRVKMDIKLYECY 182

BLAST of Lag0019274 vs. ExPASy Swiss-Prot
Match: Q9SUQ8 (Dirigent protein 6 OS=Arabidopsis thaliana OX=3702 GN=DIR6 PE=1 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 7.9e-44
Identity = 90/185 (48.65%), Postives = 114/185 (61.62%), Query Frame = 0

Query: 3   FSAKVCILLVLFLFSSSSSSAFPGKKQKPKPCKELVLYFHDIIYNGTNAANATSAIVAAP 62
           F A     L++ LFS +  S F     + KPCK    YFHDI+Y+G N ANATSA + +P
Sbjct: 10  FKALFSFFLLVLLFSDTVLS-FRKTIDQKKPCKHFSFYFHDILYDGDNVANATSAAIVSP 69

Query: 63  EGSNLTILAPHNHFGNIVVFDDPLTLDNNLHSPPVGRAQGLYLYDTKNTFTAWFGFTFSL 122
            G        +  FG  V+FD P+T+D N  S PV RAQG Y YD K  F +WF +T   
Sbjct: 70  PGLG------NFKFGKFVIFDGPITMDKNYLSKPVARAQGFYFYDMKMDFNSWFSYTLVF 129

Query: 123 NYTSYVGTINCIGADPALVKTRDISVVGGTGDFFMHRGIATIMTDAVEDDVYFRLRFDIK 182
           N T + GT+N +GAD  +  TRD+SVVGGTGDFFM RGIAT +TD  +   YFR++ DIK
Sbjct: 130 NSTEHKGTLNIMGADLMMEPTRDLSVVGGTGDFFMARGIATFVTDLFQGAKYFRVKMDIK 187

Query: 183 FYECW 188
            YEC+
Sbjct: 190 LYECY 187

BLAST of Lag0019274 vs. ExPASy Swiss-Prot
Match: Q9T019 (Dirigent protein 14 OS=Arabidopsis thaliana OX=3702 GN=DIR14 PE=2 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 2.9e-38
Identity = 80/179 (44.69%), Postives = 104/179 (58.10%), Query Frame = 0

Query: 9   ILLVLFLFSSSSSSAFPGKKQKPKPCKELVLYFHDIIYNGTNAANATSAIVAAPEGSNLT 68
           I L + L  S + S+F       KPCK  VL+ HDI Y+G NAANATSA +  P G    
Sbjct: 11  ICLSVLLCQSYTVSSFQKSLDLAKPCKRFVLHLHDIAYDGDNAANATSAAIVNPLGLG-- 70

Query: 69  ILAPHNHFGNIVVFDDPLTLDNNLHSPPVGRAQGLYLYDTKNTFTAWFGFTFSLNYTSYV 128
                  FG  V+ DDP+T+D N  S PV R QG + Y  K T+ AW  +T   N T + 
Sbjct: 71  ----DFSFGKFVIMDDPVTMDQNYLSKPVARVQGFFCYHGKATYDAWIAWTVVFNSTQHK 130

Query: 129 GTINCIGADPALVKTRDISVVGGTGDFFMHRGIATIMTDAVEDDVYFRLRFDIKFYECW 188
           G    +G +P +  TRD+ +VGGTGDF M RGIAT+ TD ++   YFR++ DIK YEC+
Sbjct: 131 GAFTIMGENPFMEPTRDLPIVGGTGDFIMTRGIATLTTDHIDGSKYFRVKLDIKLYECY 183

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6577167.11.5e-8484.13Disease resistance response protein 206, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022931436.12.0e-8484.13disease resistance response protein 206-like [Cucurbita moschata][more]
XP_022985425.12.0e-8484.66disease resistance response protein 206-like [Cucurbita maxima][more]
XP_023553111.12.9e-8383.07disease resistance response protein 206-like [Cucurbita pepo subsp. pepo][more]
XP_038906438.13.7e-8382.54disease resistance response protein 206-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
P132405.6e-6668.33Disease resistance response protein 206 OS=Pisum sativum OX=3888 GN=PI206 PE=1 S... [more]
Q1ZZU93.6e-6562.77Dirigent protein OS=Sinopodophyllum hexandrum OX=93608 GN=DIR PE=2 SV=1[more]
Q9SH661.9e-4549.44Dirigent protein 5 OS=Arabidopsis thaliana OX=3702 GN=DIR5 PE=2 SV=1[more]
Q9SUQ87.9e-4448.65Dirigent protein 6 OS=Arabidopsis thaliana OX=3702 GN=DIR6 PE=1 SV=1[more]
Q9T0192.9e-3844.69Dirigent protein 14 OS=Arabidopsis thaliana OX=3702 GN=DIR14 PE=2 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004265Dirigent proteinPFAMPF03018Dirigentcoord: 34..181
e-value: 1.4E-34
score: 119.0
IPR044859Allene oxide cyclase/Dirigent proteinGENE3D2.40.480.10coord: 20..183
e-value: 7.1E-12
score: 47.2
NoneNo IPR availablePANTHERPTHR46442:SF16DIRIGENT PROTEINcoord: 8..187
NoneNo IPR availablePANTHERPTHR46442DIRIGENT PROTEINcoord: 8..187

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0019274.1Lag0019274.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0048046 apoplast