Lag0019229 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0019229
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionsubtilisin-like protease SBT3.7
Locationchr5: 40012457 .. 40013154 (+)
RNA-Seq ExpressionLag0019229
SyntenyLag0019229
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTAAGGTTGGGGCGAGCAAAAACCATCATCGGCAAGCCTATATCAACCAGGATAGTCTATTTCTCATCTAGAGGTCCAAATTCTGCTTCACCTGCAATTCTCAAGGTGCATCTTACATAGTTGATATATTCTCTAATATCAAATCATATGATAACATCAATCTATATTCTATTGTCTAAATGACAAATTAATAAACAATAATAACGATAGTGGCTTATTAGAATAAAAAATATTTGGCTGCATAGTCGTGAGTAGGAATCTCCCTACCCACCCCATTAAAATCTTAACAACTAAGAAAGAGAAAAGTAGAAAATGATAAATTTATTTTATCTCTCCAGATTGTTGACGTTTTAATGATAGTGTGCAGGGAGATCATTACATACACCGATATAGGAGCCGAATATTTCCTATTAGAGTTATATTAAAAGCTAAAAAACTAACTAAACTTTTAAAATTTTACAAGTAATTATTCTCGTGAGCAGCCAGATATAGCCGCTCCAGGATCTAATATTCTAGCCGCCATTTCACCCCACCACTCGTCCAACGACAAAGGGTTTGGAATGATGTCGGGAACTTCCATGGCCGCACCTCATATCTCTGGTATCGTGGCTCTTCTTAGATCCCTCCGTCCTACTTGGTCACCTGCTGCAATCAAATCTGCCCTCATCACCACTGGTATGCAGCTTAAGCGTTGA

mRNA sequence

ATGGTAAGGTTGGGGCGAGCAAAAACCATCATCGGCAAGCCTATATCAACCAGGATAGTCTATTTCTCATCTAGAGGTCCAAATTCTGCTTCACCTGCAATTCTCAAGCCAGATATAGCCGCTCCAGGATCTAATATTCTAGCCGCCATTTCACCCCACCACTCGTCCAACGACAAAGGGTTTGGAATGATGTCGGGAACTTCCATGGCCGCACCTCATATCTCTGGTATCGTGGCTCTTCTTAGATCCCTCCGTCCTACTTGGTCACCTGCTGCAATCAAATCTGCCCTCATCACCACTGGTATGCAGCTTAAGCGTTGA

Coding sequence (CDS)

ATGGTAAGGTTGGGGCGAGCAAAAACCATCATCGGCAAGCCTATATCAACCAGGATAGTCTATTTCTCATCTAGAGGTCCAAATTCTGCTTCACCTGCAATTCTCAAGCCAGATATAGCCGCTCCAGGATCTAATATTCTAGCCGCCATTTCACCCCACCACTCGTCCAACGACAAAGGGTTTGGAATGATGTCGGGAACTTCCATGGCCGCACCTCATATCTCTGGTATCGTGGCTCTTCTTAGATCCCTCCGTCCTACTTGGTCACCTGCTGCAATCAAATCTGCCCTCATCACCACTGGTATGCAGCTTAAGCGTTGA

Protein sequence

MVRLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKPDIAAPGSNILAAISPHHSSNDKGFGMMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITTGMQLKR
Homology
[Close]
BLAST of Lag0019229 vs. NCBI nr
Match: XP_022159381.1 (subtilisin-like protease SBT3.4, partial [Momordica charantia])

HSP 1 Score: 158.7 bits (400), Expect = 2.8e-35
Identity = 80/100 (80.00%), Postives = 89/100 (89.00%), Query Frame = 0 

Query: 1   MVRLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKPDIAAPGSNILAAISPHHSSNDKG 60
           MVR+GRA TI+GKPIST + +FSSRGPNSASPAILKPDIAAPGSNILAAISP +  +DKG
Sbjct: 382 MVRIGRAXTIVGKPISTNVAFFSSRGPNSASPAILKPDIAAPGSNILAAISPRNPFSDKG 441

Query: 61  FGMMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITT 101
           F   SGTSMAAPHISGIVALL+SL PTWSPAAI+SA+ TT
Sbjct: 442 FAFQSGTSMAAPHISGIVALLKSLHPTWSPAAIRSAJSTT 481

[Close]
BLAST of Lag0019229 vs. NCBI nr
Match: XP_022931498.1 (subtilisin-like protease SBT3.7 isoform X6 [Cucurbita moschata])

HSP 1 Score: 157.1 bits (396), Expect = 8.1e-35
Identity = 77/100 (77.00%), Postives = 87/100 (87.00%), Query Frame = 0 

Query: 1   MVRLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKPDIAAPGSNILAAISPHHSSNDKG 60
           ++RL   +TI+GKPIS+RI YFSSRGPNS SP ILKPDIAAPGSNI+AA+ P+H   DKG
Sbjct: 470 LIRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGGDKG 529

Query: 61  FGMMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITT 101
           F  MSGTSMA PHISGIVAL++SLRPTWSPAAIKSALITT
Sbjct: 530 FAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITT 569

[Close]
BLAST of Lag0019229 vs. NCBI nr
Match: XP_022931496.1 (subtilisin-like protease SBT3.7 isoform X4 [Cucurbita moschata])

HSP 1 Score: 157.1 bits (396), Expect = 8.1e-35
Identity = 77/100 (77.00%), Postives = 87/100 (87.00%), Query Frame = 0 

Query: 1   MVRLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKPDIAAPGSNILAAISPHHSSNDKG 60
           ++RL   +TI+GKPIS+RI YFSSRGPNS SP ILKPDIAAPGSNI+AA+ P+H   DKG
Sbjct: 472 LIRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGGDKG 531

Query: 61  FGMMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITT 101
           F  MSGTSMA PHISGIVAL++SLRPTWSPAAIKSALITT
Sbjct: 532 FAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITT 571

[Close]
BLAST of Lag0019229 vs. NCBI nr
Match: XP_022931495.1 (subtilisin-like protease SBT3.7 isoform X3 [Cucurbita moschata])

HSP 1 Score: 157.1 bits (396), Expect = 8.1e-35
Identity = 77/100 (77.00%), Postives = 87/100 (87.00%), Query Frame = 0 

Query: 1   MVRLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKPDIAAPGSNILAAISPHHSSNDKG 60
           ++RL   +TI+GKPIS+RI YFSSRGPNS SP ILKPDIAAPGSNI+AA+ P+H   DKG
Sbjct: 474 LIRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGGDKG 533

Query: 61  FGMMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITT 101
           F  MSGTSMA PHISGIVAL++SLRPTWSPAAIKSALITT
Sbjct: 534 FAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITT 573

[Close]
BLAST of Lag0019229 vs. NCBI nr
Match: XP_022931497.1 (subtilisin-like protease SBT3.7 isoform X5 [Cucurbita moschata])

HSP 1 Score: 157.1 bits (396), Expect = 8.1e-35
Identity = 77/100 (77.00%), Postives = 87/100 (87.00%), Query Frame = 0 

Query: 1   MVRLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKPDIAAPGSNILAAISPHHSSNDKG 60
           ++RL   +TI+GKPIS+RI YFSSRGPNS SP ILKPDIAAPGSNI+AA+ P+H   DKG
Sbjct: 472 LIRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGGDKG 531

Query: 61  FGMMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITT 101
           F  MSGTSMA PHISGIVAL++SLRPTWSPAAIKSALITT
Sbjct: 532 FAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITT 571

[Close]
BLAST of Lag0019229 vs. ExPASy Swiss-Prot
Match: Q9ZSB0 (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 3.3e-31
Identity = 67/100 (67.00%), Postives = 83/100 (83.00%), Query Frame = 0 

Query: 1   MVRLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKPDIAAPGSNILAAISPHHSSNDKG 60
           +V++  +KT+ G+ +ST++  FSSRGPNS SPAILKPDIAAPG NILAAISP+ S ND G
Sbjct: 467 IVKIQASKTLFGQSVSTKVATFSSRGPNSVSPAILKPDIAAPGVNILAAISPNSSINDGG 526

Query: 61  FGMMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITT 101
           F MMSGTSMA P +SG+V LL+SL P WSP+AIKSA++TT
Sbjct: 527 FAMMSGTSMATPVVSGVVVLLKSLHPDWSPSAIKSAIVTT 566

[Close]
BLAST of Lag0019229 vs. ExPASy Swiss-Prot
Match: Q9ZSB1 (Subtilisin-like protease SBT3.10 OS=Arabidopsis thaliana OX=3702 GN=SBT3.10 PE=3 SV=2)

HSP 1 Score: 129.4 bits (324), Expect = 2.4e-29
Identity = 66/100 (66.00%), Postives = 82/100 (82.00%), Query Frame = 0 

Query: 1   MVRLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKPDIAAPGSNILAAISPHHSSNDKG 60
           +V +  ++T+ G+ +ST++  FSSRGPNS SPAILKPDIAAPG NILAAISP +S ND G
Sbjct: 471 IVNIQASRTLFGQSVSTKVATFSSRGPNSVSPAILKPDIAAPGVNILAAISP-NSINDGG 530

Query: 61  FGMMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITT 101
           F MMSGTSMA P +SG+V LL+SL P WSP+AIKSA++TT
Sbjct: 531 FAMMSGTSMATPVVSGVVVLLKSLHPDWSPSAIKSAIVTT 569

[Close]
BLAST of Lag0019229 vs. ExPASy Swiss-Prot
Match: Q9MAP4 (Subtilisin-like protease SBT3.2 OS=Arabidopsis thaliana OX=3702 GN=SBT3.2 PE=2 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 9.1e-29
Identity = 62/100 (62.00%), Postives = 82/100 (82.00%), Query Frame = 0 

Query: 1   MVRLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKPDIAAPGSNILAAISPHHSSNDKG 60
           + ++   +T++G P++T++  FSSRGPNS SPAILKPDIAAPG NILAA SP+ +  DKG
Sbjct: 445 VAKIQPTRTLVGLPVATKVATFSSRGPNSISPAILKPDIAAPGVNILAATSPNDTFYDKG 504

Query: 61  FGMMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITT 101
           F M SGTSM+AP ++GIVALL+S+ P WSPAAI+SA++TT
Sbjct: 505 FAMKSGTSMSAPVVAGIVALLKSVHPHWSPAAIRSAIVTT 544

[Close]
BLAST of Lag0019229 vs. ExPASy Swiss-Prot
Match: Q9MAP5 (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 9.1e-29
Identity = 62/100 (62.00%), Postives = 82/100 (82.00%), Query Frame = 0 

Query: 1   MVRLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKPDIAAPGSNILAAISPHHSSNDKG 60
           +V++ R++T+ G+P+ T++V FSSRGPNS SPAILKPDIAAPG  ILAA SP+ + N  G
Sbjct: 488 VVKIQRSRTLSGQPVGTKVVNFSSRGPNSMSPAILKPDIAAPGVRILAATSPNDTLNVGG 547

Query: 61  FGMMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITT 101
           F M+SGTSMA P ISG++ALL++L P WSPAA +SA++TT
Sbjct: 548 FAMLSGTSMATPVISGVIALLKALHPEWSPAAFRSAIVTT 587

[Close]
BLAST of Lag0019229 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 2.0e-28
Identity = 62/100 (62.00%), Postives = 82/100 (82.00%), Query Frame = 0 

Query: 1   MVRLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKPDIAAPGSNILAAISPHHSSNDKG 60
           +V++  ++T++G+P+ T++  FSSRGPNS SPAILKPDIAAPG +ILAA SP+ + N  G
Sbjct: 484 VVKIQPSRTLVGEPVGTKVATFSSRGPNSISPAILKPDIAAPGVSILAATSPNDTLNAGG 543

Query: 61  FGMMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITT 101
           F M SGTSMAAP ISG++ALL+SL P WSPAA +SA++TT
Sbjct: 544 FVMRSGTSMAAPVISGVIALLKSLHPDWSPAAFRSAIVTT 583

The following BLAST results are available for this feature:
BLAST of Lag0019229 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022159381.12.8e-3580.00subtilisin-like protease SBT3.4, partial [Momordica charantia][more]
XP_022931498.18.1e-3577.00subtilisin-like protease SBT3.7 isoform X6 [Cucurbita moschata][more]
XP_022931496.18.1e-3577.00subtilisin-like protease SBT3.7 isoform X4 [Cucurbita moschata][more]
XP_022931495.18.1e-3577.00subtilisin-like protease SBT3.7 isoform X3 [Cucurbita moschata][more]
XP_022931497.18.1e-3577.00subtilisin-like protease SBT3.7 isoform X5 [Cucurbita moschata][more]
BLAST of Lag0019229 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9ZSB03.3e-3167.00Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 S... [more]
Q9ZSB12.4e-2966.00Subtilisin-like protease SBT3.10 OS=Arabidopsis thaliana OX=3702 GN=SBT3.10 PE=3... [more]
Q9MAP49.1e-2962.00Subtilisin-like protease SBT3.2 OS=Arabidopsis thaliana OX=3702 GN=SBT3.2 PE=2 S... [more]
Q9MAP59.1e-2962.00Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 S... [more]
F4HPF12.0e-2862.00Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 S... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 12..104
e-value: 1.4E-38
score: 134.9
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 16..104
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 18..100
e-value: 6.7E-23
score: 81.3
NoneNo IPR availablePANTHERPTHR43806:SF50SUBTILISIN-LIKE PROTEASE SBT3.1-RELATEDcoord: 2..103
NoneNo IPR availablePANTHERPTHR43806PEPTIDASE S8coord: 2..103
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 1..106
score: 16.815746
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 66..76

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0019229.1Lag0019229.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity