Lag0019041 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0019041
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPapain family cysteine protease
Locationchr5: 37919677 .. 37920758 (-)
RNA-Seq ExpressionLag0019041
SyntenyLag0019041
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGGTTTGTTTTATAAGTTGTTTTTTTGTTTCTTAAATTTCCAGCCTAAGTTTTTTCATAAATTCTGATGCTTTTTTCTTTCCCATGTCACAAATAATAGGAGGTAACATCTTTTGATTGGAGAGACCATAATGTTCTTACACCCGTTCGAAATCAGAAACGATCCCGTATGTATTTTATTTTCTTATTAGTAAACCGTAATATATATTATCTCTACCTTTTATTAATGTCTGATTAATTAATTAATTATATATATCTTTTTTAATTTGTTCAGGATTTTGCTGGGCTATCGTGGCCGCAGGAGCCATCGAGTCGTTGCATAACATTGAGCATGGGAACAACAATCTGCAGCTTTCACCTCAGTACTTAATCAATTGTATCATATGTCCTAATGTGTATGAGGTCGAGATAGAGAGTAAGGAGATTGGTTATATACTGTCAATTCCAAAAACAGGTGGATAATGAAAAATGGTATTCCTCAAGAGTCCGAGGTTCCATTTGTAGCAGAAATAGGAGCATGTAGGCGTATGACTGGGGTATATTTACAGTTTCTCACTTAATTAACTTACCCTCCATCTTTTATATAATTATATATTACTAGCTAACTGTTAATATTTTATTTCATTTTTAATTTAAGCATCTCCTCAAAATTCAAAATTTTGTAAAGATACGAGATTTTTCCAATTTGAACCTACACATTCAACGACAACCAATAGCAGCGGGTGTAAGGCTTTGTTTGGAATTTCAACAGTGACAACTGTAAGTAAATATATTACACTCGTATTTACACGTGATAAATATTTGCTACTAACTTTATTTTGTTAATGATAAATTTAGGAGATTTACAAAGGACCAACAGATTGGAATGCATGTTTAACGAAGGTTGCCACGCAGTTTTTGTTGTTGGGTTCGACATCGATTCTACTGGCCAGAAATACTGGATTGTCAAGAACTCGTGGGGCGAGGAAGGGGGAGAATGTGGCTATGGGAAAATTAGTCAAGAGATTGTCCATACTCTGAGAGGCTCCAAATACTTGATAGAGATGTTAACTTATCCAACCGATATTGTGCTCAACTAA

mRNA sequence

ATGCAGGAGGTAACATCTTTTGATTGGAGAGACCATAATGTTCTTACACCCGTTCGAAATCAGAAACGATCCCGATTTTGCTGGGCTATCGTGGCCGCAGGAGCCATCGAGTCGTTGCATAACATTGAGCATGGGAACAACAATCTGCAGCTTTCACCTCAGTACTTAATCAATTGTATCATATGTCCTAATGTGTATGAGGTCGAGATAGAGAGTAAGGAGATTGGTTATATACTGTCAATTCCAAAAACAGGAGATTTACAAAGGACCAACAGATTGGAATGCATGTTTAACGAAGGTTGCCACGCAGTTTTTGTTGTTGGGTTCGACATCGATTCTACTGGCCAGAAATACTGGATTGTCAAGAACTCGTGGGGCGAGGAAGGGGGAGAATGTGGCTATGGGAAAATTAGTCAAGAGATTGTCCATACTCTGAGAGGCTCCAAATACTTGATAGAGATGTTAACTTATCCAACCGATATTGTGCTCAACTAA

Coding sequence (CDS)

ATGCAGGAGGTAACATCTTTTGATTGGAGAGACCATAATGTTCTTACACCCGTTCGAAATCAGAAACGATCCCGATTTTGCTGGGCTATCGTGGCCGCAGGAGCCATCGAGTCGTTGCATAACATTGAGCATGGGAACAACAATCTGCAGCTTTCACCTCAGTACTTAATCAATTGTATCATATGTCCTAATGTGTATGAGGTCGAGATAGAGAGTAAGGAGATTGGTTATATACTGTCAATTCCAAAAACAGGAGATTTACAAAGGACCAACAGATTGGAATGCATGTTTAACGAAGGTTGCCACGCAGTTTTTGTTGTTGGGTTCGACATCGATTCTACTGGCCAGAAATACTGGATTGTCAAGAACTCGTGGGGCGAGGAAGGGGGAGAATGTGGCTATGGGAAAATTAGTCAAGAGATTGTCCATACTCTGAGAGGCTCCAAATACTTGATAGAGATGTTAACTTATCCAACCGATATTGTGCTCAACTAA

Protein sequence

MQEVTSFDWRDHNVLTPVRNQKRSRFCWAIVAAGAIESLHNIEHGNNNLQLSPQYLINCIICPNVYEVEIESKEIGYILSIPKTGDLQRTNRLECMFNEGCHAVFVVGFDIDSTGQKYWIVKNSWGEEGGECGYGKISQEIVHTLRGSKYLIEMLTYPTDIVLN
Homology
BLAST of Lag0019041 vs. NCBI nr
Match: KDO63024.1 (hypothetical protein CISIN_1g018958mg [Citrus sinensis])

HSP 1 Score: 94.7 bits (234), Expect = 7.7e-16
Identity = 53/141 (37.59%), Postives = 71/141 (50.35%), Query Frame = 0

Query: 5   TSFDWRDHNVLTPVRNQKRSRFCWAIVAAGAIESLHNIEHGNNNLQLSPQYLINCIICPN 64
           TS DWRD   +TP++NQK    CWA  A  A+E +  I  G N +QLS Q L++C    N
Sbjct: 138 TSLDWRDKGAVTPIKNQKECGCCWAFAAVAAVEGITKIRSG-NLIQLSEQQLLDCSTNGN 197

Query: 65  VYEVEIESKEIGYILSIPKTGDLQRTNRLECMFNEGC-----HAVFVVGFDIDSTGQKYW 124
              +   S+E  +   I   G          +FN  C     HAV +VGF     G  YW
Sbjct: 198 NGCLG-GSREKAFAYIIQNQG----------IFNGVCGTQLDHAVTIVGFGTTEDGANYW 257

Query: 125 IVKNSWGEEGGECGYGKISQE 141
           ++KNSWG   G+ GY KI ++
Sbjct: 258 LIKNSWGNTWGDAGYMKIVRD 266

BLAST of Lag0019041 vs. NCBI nr
Match: OBS76496.1 (hypothetical protein A6R68_17052, partial [Neotoma lepida])

HSP 1 Score: 87.4 bits (215), Expect = 1.2e-13
Identity = 50/165 (30.30%), Postives = 79/165 (47.88%), Query Frame = 0

Query: 6   SFDWRDHNVLTPVRNQKRSRFCWAIVAAGAIESLHNIEHGNNNLQLSPQYLINCI----- 65
           S DWR H+ +TPV++Q +   CWA  A G++E           + LS Q L++C      
Sbjct: 160 SVDWRKHSYVTPVKDQGKCGACWAFSAVGSLEG-QMFRKTGKLVPLSEQNLVDCSWSYGN 219

Query: 66  ------ICPNVYEVEIESKEIGYILSIPKTGDLQRTNRLECMFNEGCHAVFVVGFDIDST 125
                 +    ++   ++  +    S P    +       C  N   HA+ VVG+  +S 
Sbjct: 220 KGCDGGLMEPAFQYVKDNGGLDTRESYPYEARVSMYYEANCSSNNLDHAMLVVGYGEESD 279

Query: 126 GQKYWIVKNSWGEEGGECGYGKISQEIVHTLRGSKYLIEMLTYPT 160
           G+KYW+VKNSWGE+ G  GY K++++  +    + Y I    YPT
Sbjct: 280 GKKYWLVKNSWGEDWGMSGYIKMARDRNNNCGIASYAI----YPT 319

BLAST of Lag0019041 vs. NCBI nr
Match: XP_012647628.1 (Papain family cysteine protease [Babesia microti strain RI] >CCF73019.1 Papain family cysteine protease [Babesia microti strain RI])

HSP 1 Score: 87.4 bits (215), Expect = 1.2e-13
Identity = 55/196 (28.06%), Postives = 87/196 (44.39%), Query Frame = 0

Query: 7   FDWRDHNVLTPVRNQKRSRFCWAIVAAGAIESLHNIEHGNNNLQLSPQYLINCI----IC 66
           FDWRD +V++PVR Q+    CWAI AAGAI++++NI++  + +  SPQ+L+NC+     C
Sbjct: 328 FDWRDQDVISPVRAQQGCGSCWAIAAAGAIDAVYNIKNKGSKMITSPQHLMNCVSDEFTC 387

Query: 67  P--NVYEVEIESKEI--GYIL--SIPKTGDLQRTNRLEC--------------------- 126
               V  + IE  ++  G  +   +P   + Q+     C                     
Sbjct: 388 QTGGVVRMAIEYAQVKGGVCVESDVPYVAEKQKCETKACKQLVTISKYFKVPANKMQSVL 447

Query: 127 ----------------------MFNEGC-----HAVFVVGFDI-DSTGQKYWIVKNSWGE 144
                                 ++N  C     HA+ + G+   DS   +YWI KNSWG 
Sbjct: 448 KDKGPIAAAMAITKDFLQYESGVYNGSCNKVLNHAMLITGYGYDDSVNSRYWIFKNSWGS 507

BLAST of Lag0019041 vs. NCBI nr
Match: OAE33067.1 (hypothetical protein AXG93_1913s1690 [Marchantia polymorpha subsp. ruderalis])

HSP 1 Score: 86.7 bits (213), Expect = 2.1e-13
Identity = 57/184 (30.98%), Postives = 83/184 (45.11%), Query Frame = 0

Query: 5   TSFDWRDHNVLTPVRNQKRSRFCWAIVAAGAIESLHNIEHGNNNLQLSPQYLINCIICPN 64
           T  DWR    +T V++Q +   CWA    GA+ESL+ I+ G N + LS Q L++C +  N
Sbjct: 134 TEVDWRKEGAVTGVKSQGQCGSCWAFSTIGAVESLNQIKTG-NLISLSEQELVDCDVTLN 193

Query: 65  VYEVEIESKEIGYILSIPKTG----------------DLQRTNRL--------ECMFNEG 124
            Y       + G+   +   G                +  +  R+        + ++N  
Sbjct: 194 TYGCAGGFMDYGFEYIVQNGGVDTDMDYPYIAAEDFCNSHKETRVAASINAYEQGIYNTT 253

Query: 125 C-----HAVFVVGFDIDSTGQKYWIVKNSWGEEGGECGYGKISQEIVHTLRGSKYLIEML 160
           C     H V VVG+  D  GQ+YW+VKNSWGE  GE GY ++ Q  V    G   +    
Sbjct: 254 CGTELDHGVLVVGYGTDQ-GQEYWLVKNSWGESWGESGYIRL-QRNVQAPEGMCGIAMAA 313

BLAST of Lag0019041 vs. NCBI nr
Match: XP_006421530.1 (ervatamin-B [Citrus clementina] >ESR34770.1 hypothetical protein CICLE_v10005334mg [Citrus clementina])

HSP 1 Score: 86.3 bits (212), Expect = 2.7e-13
Identity = 56/198 (28.28%), Postives = 81/198 (40.91%), Query Frame = 0

Query: 5   TSFDWRDHNVLTPVRNQKRSRFCWAIVAAGAIESLHNIEHGNNNLQLSPQYLINCII--- 64
           TS DWR+   +TP++NQ +   CWA  A  A+E +  I  G N + LS Q +++C I   
Sbjct: 130 TSMDWREKGAVTPIKNQGQCGVCWAFSAVAAVEGVTKIS-GGNLIPLSEQQILDCSIDGN 189

Query: 65  -------CPNVYEVEIESKEIGYILSIP-------------------------KTGDLQR 124
                    N ++  I+++ I      P                         K  D Q 
Sbjct: 190 RGCDGGWMDNAFKYIIKNQGIATEADYPYKEVQGTCEDAQVKVAAKISNFEDVKPNDEQA 249

Query: 125 TNRLECM---------------------FNEGC-----HAVFVVGFDIDSTGQKYWIVKN 142
             +   M                     FN GC     HAV +VGF     G KYW++KN
Sbjct: 250 LLQAVAMQPVSICIEGSGPDFQSYKGGIFNRGCGTQCSHAVAIVGFGATEDGMKYWLIKN 309

BLAST of Lag0019041 vs. ExPASy Swiss-Prot
Match: P80884 (Ananain OS=Ananas comosus OX=4615 GN=AN1 PE=1 SV=2)

HSP 1 Score: 79.3 bits (194), Expect = 4.4e-14
Identity = 52/194 (26.80%), Postives = 81/194 (41.75%), Query Frame = 0

Query: 6   SFDWRDHNVLTPVRNQKRSRFCWAIVAAGAIESLHNIEHGNNNLQLSPQYLINCII---- 65
           S DWRD   +T V+NQ R   CWA  +   +ES++ I+ G N + LS Q +++C +    
Sbjct: 126 SIDWRDSGAVTSVKNQGRCGSCWAFASIATVESIYKIKRG-NLVSLSEQQVLDCAVSYGC 185

Query: 66  ----CPNVYEVEIESKEIGYILSIP--------KTGD------------LQRTNRLECM- 125
                   Y   I +K +      P        KT              +QR N    M 
Sbjct: 186 KGGWINKAYSFIISNKGVASAAIYPYKAAKGTCKTNGVPNSAYITRYTYVQRNNERNMMY 245

Query: 126 ------------------------FNEGC-----HAVFVVGFDIDSTGQKYWIVKNSWGE 142
                                   F   C     HA+ ++G+  DS+G+K+WIV+NSWG 
Sbjct: 246 AVSNQPIAAALDASGNFQHYKRGVFTGPCGTRLNHAIVIIGYGQDSSGKKFWIVRNSWGA 305

BLAST of Lag0019041 vs. ExPASy Swiss-Prot
Match: Q9PYY5 (Viral cathepsin OS=Xestia c-nigrum granulosis virus OX=51677 GN=VCATH PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.7e-13
Identity = 57/194 (29.38%), Postives = 79/194 (40.72%), Query Frame = 0

Query: 6   SFDWRDHNVLTPVRNQKRSRFCWAIVAAGAIESLHNIEHGNNNLQLSPQYLINCIICPN- 65
           SFDWRD N +T V+ QK    CWA  A   IESL++I+H N +L LS Q L++C    N 
Sbjct: 136 SFDWRDRNSVTSVKMQKECGSCWAFSAVANIESLYHIKH-NVSLDLSEQQLVDCDKVNNG 195

Query: 66  --------VYEVEIESKEIGYILSIPKTG--------------------DLQRTNRL--- 125
                    +E  I +  I Y    P TG                    DL+   +L   
Sbjct: 196 CNGGLMSWAFEGIIRAGGISYEAPYPYTGVDGVCKNTTRYVQLSGCYAYDLRSEKKLRQV 255

Query: 126 -------------------------ECMFNEGC-HAVFVVGFDIDSTGQKYWIVKNSWGE 142
                                     C  + G  H V +VG+       KYW +KNSWG 
Sbjct: 256 LHEKGPVSVAIDVVDLTNYKSGVAKHCSVDHGLNHGVLLVGYG-QENDVKYWTLKNSWGS 315

BLAST of Lag0019041 vs. ExPASy Swiss-Prot
Match: O91466 (Viral cathepsin OS=Cydia pomonella granulosis virus (isolate Mexico/1963) OX=654905 GN=VCATH PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.4e-12
Identity = 57/193 (29.53%), Postives = 80/193 (41.45%), Query Frame = 0

Query: 6   SFDWRDHNVLTPVRNQKRSRFCWAIVAAGAIESLHNIEHGNNNLQLSPQYLINCIICPN- 65
           + DWRD + +TPV+NQ     CWA      IESL+NI++ +  L LS Q+L+NC    N 
Sbjct: 127 TLDWRDKHGVTPVKNQMECGSCWAFSTIANIESLYNIKY-DKALNLSEQHLVNCDNINNG 186

Query: 66  --------------------------VYEVEIESKEIGYILSI--PKTGDLQRTNRLE-- 125
                                      Y  +   K+  + LSI   +   LQ  N+L   
Sbjct: 187 CAGGLMHWALESILQEGGVVSAENEPYYGFDGVCKKSPFELSISGSRRYVLQNENKLREL 246

Query: 126 --------------------------CMFNEGC-HAVFVVGFDIDSTGQKYWIVKNSWGE 141
                                     C  NEG  HAV +VG+ +      YWI+KNSWG 
Sbjct: 247 LVVNGPISVAIDVSDLINYKAGIADICENNEGLNHAVLLVGYGV-KNDVPYWILKNSWGA 306

BLAST of Lag0019041 vs. ExPASy Swiss-Prot
Match: O97397 (Cathepsin L-like proteinase OS=Phaedon cochleariae OX=80249 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.4e-12
Identity = 56/215 (26.05%), Postives = 85/215 (39.53%), Query Frame = 0

Query: 6   SFDWRDHNVLTPVRNQKRSRFCWAIVAAGAIESLHNIEHGNNNLQLSPQYLINCII---- 65
           S DWR   V+ PVRNQ     CWA+  A AIES   I+ G + + LSPQ L++C      
Sbjct: 113 SIDWRSKGVVLPVRNQGECGSCWALSTAAAIESQSAIKSG-SKVPLSPQQLVDCSTSYGN 172

Query: 66  --CPNVYEV------------------------------------------EIESKEIGY 125
             C   + V                                          ++ + E   
Sbjct: 173 HGCNGGFAVNGFEYVKDNGLESDADYPYSGKEDKCKANDKSRSVVELTGYKKVTASETSL 232

Query: 126 ILSIPKTGDLQRT--------------NRLECMFNEGCHAVFVVGFDIDSTGQKYWIVKN 159
             ++   G +                 +   C+ +   H V VVG+ I++ GQKYWI+KN
Sbjct: 233 KEAVGTIGPISAVVFGKPMKSYGGGIFDDSSCLGDNLHHGVNVVGYGIEN-GQKYWIIKN 292

BLAST of Lag0019041 vs. ExPASy Swiss-Prot
Match: Q9FGR9 (KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 PE=1 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 4.1e-12
Identity = 56/201 (27.86%), Postives = 79/201 (39.30%), Query Frame = 0

Query: 5   TSFDWRDHNVLTPVRNQKRSRFCWAIVAAGAIESLHNIEHGNNNLQLSPQYLINCIICPN 64
           TS DWR +  +TPV+NQ +   CWA     A+E ++ I        LS Q L++C    N
Sbjct: 128 TSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQI-RTKKLTSLSEQELVDCDTNQN 187

Query: 65  -------------------------VYEVEIE------SKEIGYILSIPKTGDLQRTNRL 124
                                    VY  +        +KE   ++SI    D+ + +  
Sbjct: 188 QGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSIDGHEDVPKNSED 247

Query: 125 ECM---------------------FNEGC----------HAVFVVGFDIDSTGQKYWIVK 144
           + M                     ++EG           H V VVG+     G KYWIVK
Sbjct: 248 DLMKAVANQPVSVAIDAGGSDFQFYSEGVFTGRCGTELNHGVAVVGYGTTIDGTKYWIVK 307

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KDO63024.17.7e-1637.59hypothetical protein CISIN_1g018958mg [Citrus sinensis][more]
OBS76496.11.2e-1330.30hypothetical protein A6R68_17052, partial [Neotoma lepida][more]
XP_012647628.11.2e-1328.06Papain family cysteine protease [Babesia microti strain RI] >CCF73019.1 Papain f... [more]
OAE33067.12.1e-1330.98hypothetical protein AXG93_1913s1690 [Marchantia polymorpha subsp. ruderalis][more]
XP_006421530.12.7e-1328.28ervatamin-B [Citrus clementina] >ESR34770.1 hypothetical protein CICLE_v10005334... [more]
Match NameE-valueIdentityDescription
P808844.4e-1426.80Ananain OS=Ananas comosus OX=4615 GN=AN1 PE=1 SV=2[more]
Q9PYY51.7e-1329.38Viral cathepsin OS=Xestia c-nigrum granulosis virus OX=51677 GN=VCATH PE=3 SV=1[more]
O914661.4e-1229.53Viral cathepsin OS=Cydia pomonella granulosis virus (isolate Mexico/1963) OX=654... [more]
O973972.4e-1226.05Cathepsin L-like proteinase OS=Phaedon cochleariae OX=80249 PE=2 SV=1[more]
Q9FGR94.1e-1227.86KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 ... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 3..145
e-value: 2.2E-6
score: -6.5
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 94..141
e-value: 3.7E-11
score: 43.5
coord: 5..59
e-value: 1.0E-11
score: 45.4
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 2..79
e-value: 1.6E-17
score: 66.1
NoneNo IPR availableGENE3D2.40.50.170Cysteine proteinases. Chain Ccoord: 94..154
e-value: 7.2E-15
score: 56.7
NoneNo IPR availablePANTHERPTHR12411:SF553SUBFAMILY NOT NAMEDcoord: 95..143
coord: 5..61
NoneNo IPR availablePANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 95..143
coord: 5..61
IPR025660Cysteine peptidase, histidine active sitePROSITEPS00639THIOL_PROTEASE_HIScoord: 100..110
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 5..139

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0019041.1Lag0019041.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0008234 cysteine-type peptidase activity