Lag0018861 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0018861
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPlus3 domain-containing protein
Locationchr5: 35492612 .. 35494560 (+)
RNA-Seq ExpressionLag0018861
SyntenyLag0018861
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAAGAAGAAATGCAAAACGAAAGAAGAAATCGGAGAGGATTTTTGCTTCACCTGTAAAGATGGAGGACTCCTCAGATTCTGCGACTTCAAGTGAGTTCGTTTCATTACAATTGAGAATGCCCCATTCGTTGTTCTGTTCATGCAAGGATTTTCTTAGGAGGAATGAGAATCATTATGCAAGGAATAACATTATGATGTTTATGTTTAGAGAGGAGGTTCATGCATGGAAAATCGAGCGAATAGCGAAGAATGAAGTGGAAAATGGAATTGAACATTTGTTTGTTCGTTCCTTTTCTCCTTTTTCCTCGCACGATCCTGTTAGAATTAGGTTCTTAGTAGTTCGATTTTTGGCCACTTGTTTCCACATTGTAATTTTCAGCGGAAGTTTCTCCTAAAATTATTTTGGGAAAAATGCACACGGCGAAGCTTTTAGTATGATGATCAGGGTTTGTTATTTAGTGTGTGAAAGTGAAAATTTATAGAATTCTCTACCAAGTTTGAGTATCTCAATCCACATTATGTCCGGAGACGTTTCTGTTCCTTTTTGCATCCTTTCATTGGTTCAAGGAAAGTTGTTTCTATCTCTCTCTCTCTCTCTCTAAAAAAAAAAAAAAGCCCCACATGAACACTGTCAGTTTATGAATATTCATGAGTCAACATGTATGGATGCTGAAAACATATTTTTGTTAGCATATTGGTTAGGATTTTGTTTGCATGTACCGTACATGGTGATGTATTGAGACACTGTTATTAAAAGTTAAAATTACGACAGGACCATTCTGTTCTGGCTAGATTGTGTGAAGATGTGATGGAGTGGATTTTTTTCTTTAGGGTTTCATCTTTTTTCTTTTTTTAAAAAATAATTAAAAGGAATTTGGACACTCTTATATATCCCTGTTTGTCCAGATGGATATCTCTCCGATCTTGGATTTTCATTTTTTATTATAAAGATAGGCTTTTAATTGTTTATATCTTGATTTCAGAAATTGCCTTAAAGCGTATCACCCTGAATGTGTTGGAAGGGAAGATTCTTTTGTGGAGTCTGAAGATCGTTGGACTTGTGGTAAGATGCTGAACACGATTATGATTTTAAGAGTGATCTCTGAATCATGTGTGAATTTTAGTCTGTTTTAATTTAAAAGTTGCTAGTTACTCAGTTAATGATATCTTCTTAATACAAGTTATATCTTTTCACAAGGACGGGAATAAGAAGTTTATGAATCCACTATTATCTTCTGAGTTTCAAGTCTCCCCCATTTCAAGTTTCGTGACTTCTAGAGAAAGTACTGAATGAGCTTGGTATAATGTGGTTCCTGGACATGTTGGGGTCTGTCATTGACTACTACAAATTTATGAAATTAAGAGATCTATAACATGTGGATGCAAGTGAAGCATTAGTCATATTGTGCTATCTGAATATTTCTCTGACTCTTCTACTACGCTGTCTTTCTTGTTAAGGAAAAGGTTTATTAACGATTATGTGTTATTCCACAAACCTACTTGCTGCCTTGCCTGTTATATGCCATCCCATGTCTTCTACATAGTACATACACACGGAGGCCTCAATAAGATCAGTTCATAGAACTAACTGGGAACTGGGAAGTATAAGCTTACTTTTTGTCTGTTGATTTTTTTTTTGCTATATCTAGCAGTCTTTTTTTGTCTGCCTGTGCCATTTATATATTTTTCAGTTTCCTCAAATGATGATTATGTTATGTATCAGACTGGCATTCGTGCTTCCTCTGCCATAAAACCTCAAAGTTCCGTTGTGTTGGCTGCCCACAAGCTGTATGTGGTCGCTGCATTTTTAATGCCGAGTTTGTGCGTGTTCGAGGCAGTAGAGGATTCTGCAATCACTGTTTGAAGCTCACGTTACTTATAGAAGATGGTAAAGATGTTGACACTGATGGGGTATGCTCACTTGTCCTCTTTATGATTGTTTAG

mRNA sequence

ATGGGGAAGAAGAAATGCAAAACGAAAGAAGAAATCGGAGAGGATTTTTGCTTCACCTGTAAAGATGGAGGACTCCTCAGATTCTGCGACTTCAAAAATTGCCTTAAAGCGTATCACCCTGAATGTGTTGGAAGGGAAGATTCTTTTGTGGAGTCTGAAGATCGTTGGACTTGTGACTGGCATTCGTGCTTCCTCTGCCATAAAACCTCAAAGTTCCGTTGTGTTGGCTGCCCACAAGCTGTATGTGGTCGCTGCATTTTTAATGCCGAGTTTGTGCGTGTTCGAGGCAGTAGAGGATTCTGCAATCACTGTTTGAAGCTCACGTTACTTATAGAAGATGGTAAAGATGTTGACACTGATGGGGTATGCTCACTTGTCCTCTTTATGATTGTTTAG

Coding sequence (CDS)

ATGGGGAAGAAGAAATGCAAAACGAAAGAAGAAATCGGAGAGGATTTTTGCTTCACCTGTAAAGATGGAGGACTCCTCAGATTCTGCGACTTCAAAAATTGCCTTAAAGCGTATCACCCTGAATGTGTTGGAAGGGAAGATTCTTTTGTGGAGTCTGAAGATCGTTGGACTTGTGACTGGCATTCGTGCTTCCTCTGCCATAAAACCTCAAAGTTCCGTTGTGTTGGCTGCCCACAAGCTGTATGTGGTCGCTGCATTTTTAATGCCGAGTTTGTGCGTGTTCGAGGCAGTAGAGGATTCTGCAATCACTGTTTGAAGCTCACGTTACTTATAGAAGATGGTAAAGATGTTGACACTGATGGGGTATGCTCACTTGTCCTCTTTATGATTGTTTAG

Protein sequence

MGKKKCKTKEEIGEDFCFTCKDGGLLRFCDFKNCLKAYHPECVGREDSFVESEDRWTCDWHSCFLCHKTSKFRCVGCPQAVCGRCIFNAEFVRVRGSRGFCNHCLKLTLLIEDGKDVDTDGVCSLVLFMIV
Homology
BLAST of Lag0018861 vs. NCBI nr
Match: XP_022984438.1 (uncharacterized protein At5g08430-like [Cucurbita maxima])

HSP 1 Score: 255.8 bits (652), Expect = 2.1e-64
Identity = 111/121 (91.74%), Postives = 114/121 (94.21%), Query Frame = 0

Query: 1   MGKKKCKTKEEIGEDFCFTCKDGGLLRFCDFKNCLKAYHPECVGREDSFVESEDRWTCDW 60
           M KKKC  KEEIGEDFCF CKDGGLLRFCDFK+CLKAYHPECVGREDS VESEDRWTCDW
Sbjct: 1   MVKKKCTRKEEIGEDFCFICKDGGLLRFCDFKDCLKAYHPECVGREDSSVESEDRWTCDW 60

Query: 61  HSCFLCHKTSKFRCVGCPQAVCGRCIFNAEFVRVRGSRGFCNHCLKLTLLIEDGKDVDTD 120
           H+CFLCHKTSKFRCVGCPQAVCGRCIFNAEFVRVRG RGFCNHCL+LTLLIEDGKDVD D
Sbjct: 61  HACFLCHKTSKFRCVGCPQAVCGRCIFNAEFVRVRGWRGFCNHCLQLTLLIEDGKDVDID 120

Query: 121 G 122
           G
Sbjct: 121 G 121

BLAST of Lag0018861 vs. NCBI nr
Match: KAG7031352.1 (hypothetical protein SDJN02_05392, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 253.4 bits (646), Expect = 1.0e-63
Identity = 111/121 (91.74%), Postives = 113/121 (93.39%), Query Frame = 0

Query: 1   MGKKKCKTKEEIGEDFCFTCKDGGLLRFCDFKNCLKAYHPECVGREDSFVESEDRWTCDW 60
           M KKKC  KEEIGEDFCF CKDGGLLRFCDFK+CLKAYHPECVGREDS VESEDRWTCDW
Sbjct: 1   MVKKKCTRKEEIGEDFCFICKDGGLLRFCDFKDCLKAYHPECVGREDSSVESEDRWTCDW 60

Query: 61  HSCFLCHKTSKFRCVGCPQAVCGRCIFNAEFVRVRGSRGFCNHCLKLTLLIEDGKDVDTD 120
           HSCFLCHKTSKFRCV CPQAVCGRCIFNAEFVRVRG RGFCNHCL+LTLLIEDGKDVD D
Sbjct: 61  HSCFLCHKTSKFRCVCCPQAVCGRCIFNAEFVRVRGWRGFCNHCLQLTLLIEDGKDVDID 120

Query: 121 G 122
           G
Sbjct: 121 G 121

BLAST of Lag0018861 vs. NCBI nr
Match: XP_023539109.1 (uncharacterized protein At5g08430-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 253.4 bits (646), Expect = 1.0e-63
Identity = 111/121 (91.74%), Postives = 113/121 (93.39%), Query Frame = 0

Query: 1   MGKKKCKTKEEIGEDFCFTCKDGGLLRFCDFKNCLKAYHPECVGREDSFVESEDRWTCDW 60
           M KKKC  KEEIGEDFCF CKDGGLLRFCDFK+CLKAYHPECVGREDS VESEDRWTCDW
Sbjct: 1   MVKKKCTRKEEIGEDFCFICKDGGLLRFCDFKDCLKAYHPECVGREDSSVESEDRWTCDW 60

Query: 61  HSCFLCHKTSKFRCVGCPQAVCGRCIFNAEFVRVRGSRGFCNHCLKLTLLIEDGKDVDTD 120
           HSCFLCHKTSKFRCV CPQAVCGRCIFNAEFVRVRG RGFCNHCL+LTLLIEDGKDVD D
Sbjct: 61  HSCFLCHKTSKFRCVCCPQAVCGRCIFNAEFVRVRGWRGFCNHCLQLTLLIEDGKDVDID 120

Query: 121 G 122
           G
Sbjct: 121 G 121

BLAST of Lag0018861 vs. NCBI nr
Match: KAG6600713.1 (hypothetical protein SDJN03_05946, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 253.4 bits (646), Expect = 1.0e-63
Identity = 111/121 (91.74%), Postives = 113/121 (93.39%), Query Frame = 0

Query: 1   MGKKKCKTKEEIGEDFCFTCKDGGLLRFCDFKNCLKAYHPECVGREDSFVESEDRWTCDW 60
           M KKKC  KEEIGEDFCF CKDGGLLRFCDFK+CLKAYHPECVGREDS VESEDRWTCDW
Sbjct: 1   MVKKKCTRKEEIGEDFCFICKDGGLLRFCDFKDCLKAYHPECVGREDSSVESEDRWTCDW 60

Query: 61  HSCFLCHKTSKFRCVGCPQAVCGRCIFNAEFVRVRGSRGFCNHCLKLTLLIEDGKDVDTD 120
           HSCFLCHKTSKFRCV CPQAVCGRCIFNAEFVRVRG RGFCNHCL+LTLLIEDGKDVD D
Sbjct: 61  HSCFLCHKTSKFRCVCCPQAVCGRCIFNAEFVRVRGWRGFCNHCLQLTLLIEDGKDVDID 120

Query: 121 G 122
           G
Sbjct: 121 G 121

BLAST of Lag0018861 vs. NCBI nr
Match: XP_022942556.1 (uncharacterized protein At5g08430-like [Cucurbita moschata])

HSP 1 Score: 253.4 bits (646), Expect = 1.0e-63
Identity = 111/121 (91.74%), Postives = 113/121 (93.39%), Query Frame = 0

Query: 1   MGKKKCKTKEEIGEDFCFTCKDGGLLRFCDFKNCLKAYHPECVGREDSFVESEDRWTCDW 60
           M KKKC  KEEIGEDFCF CKDGGLLRFCDFK+CLKAYHPECVGREDS VESEDRWTCDW
Sbjct: 1   MVKKKCTRKEEIGEDFCFICKDGGLLRFCDFKDCLKAYHPECVGREDSSVESEDRWTCDW 60

Query: 61  HSCFLCHKTSKFRCVGCPQAVCGRCIFNAEFVRVRGSRGFCNHCLKLTLLIEDGKDVDTD 120
           HSCFLCHKTSKFRCV CPQAVCGRCIFNAEFVRVRG RGFCNHCL+LTLLIEDGKDVD D
Sbjct: 61  HSCFLCHKTSKFRCVCCPQAVCGRCIFNAEFVRVRGWRGFCNHCLQLTLLIEDGKDVDID 120

Query: 121 G 122
           G
Sbjct: 121 G 121

BLAST of Lag0018861 vs. ExPASy Swiss-Prot
Match: Q9SD34 (Zinc finger CCCH domain-containing protein 44 OS=Arabidopsis thaliana OX=3702 GN=At3g51120 PE=2 SV=3)

HSP 1 Score: 124.8 bits (312), Expect = 7.3e-28
Identity = 52/114 (45.61%), Postives = 70/114 (61.40%), Query Frame = 0

Query: 9   KEEIGEDFCFTCKDGGLLRFCDFKNCLKAYHPECVGREDSFVESEDRWTCDWHSCFLCHK 68
           KE+  ED CF C DGG L  CD +NC KAYHP C+ R+++F  +  +W C WH C  C K
Sbjct: 105 KEDKEEDVCFICFDGGDLVLCDRRNCPKAYHPACIKRDEAFFRTTAKWNCGWHICGTCQK 164

Query: 69  TSKFRCVGCPQAVCGRCIFNAEFVRVRGSRGFCNHCLKLTLLIEDGKDVDTDGV 123
            S + C  C  +VC RCI +A++V VRG+ G C  C+K  +LIE+    D + V
Sbjct: 165 ASSYMCYTCTFSVCKRCIKDADYVIVRGNMGLCGTCIKPIMLIENIAQGDNEAV 218

BLAST of Lag0018861 vs. ExPASy Swiss-Prot
Match: Q9SIV5 (Zinc finger CCCH domain-containing protein 19 OS=Arabidopsis thaliana OX=3702 GN=NERD PE=1 SV=3)

HSP 1 Score: 110.9 bits (276), Expect = 1.1e-23
Identity = 47/111 (42.34%), Postives = 65/111 (58.56%), Query Frame = 0

Query: 2   GKKKCKTKEEIGEDFCFTCKDGGLLRFCDFKNCLKAYHPECVGREDSFVESEDRWTCDWH 61
           GKKK        ED CF C DGG L  CD + C KAYHP CV R+++F +++ +W C WH
Sbjct: 594 GKKK-------EEDVCFMCFDGGDLVLCDRRGCTKAYHPSCVDRDEAFFQTKGKWNCGWH 653

Query: 62  SCFLCHKTSKFRCVGCPQAVCGRCIFNAEFVRVRGSRGFCNHCLKLTLLIE 113
            C  C KT+ + C  C  ++C  C  +A F  +RG++G C  C++   LIE
Sbjct: 654 LCSKCEKTATYLCYTCMFSLCKGCAKDAVFFCIRGNKGLCETCMETVKLIE 697

BLAST of Lag0018861 vs. ExPASy Swiss-Prot
Match: O96028 (Histone-lysine N-methyltransferase NSD2 OS=Homo sapiens OX=9606 GN=NSD2 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.1e-11
Identity = 38/105 (36.19%), Postives = 45/105 (42.86%), Query Frame = 0

Query: 3    KKKCKTKEEIG------EDFCFTCKDGGLLRFCDFKNCLKAYHPECVGREDSFVESEDRW 62
            KKK + +   G      ED CF C DGG L  CD K C KAYH  C+G          +W
Sbjct: 1222 KKKTRRRRAKGEGKRQSEDECFRCGDGGQLVLCDRKFCTKAYHLSCLGLGK---RPFGKW 1281

Query: 63   TCDWHSCFLCHKTSKFRCVGCPQAVCGRCIFNAEFVRVRGSRGFC 102
             C WH C +C K S   C  CP + C        F      R +C
Sbjct: 1282 ECPWHHCDVCGKPSTSFCHLCPNSFCKEHQDGTAFSCTPDGRSYC 1323

BLAST of Lag0018861 vs. ExPASy Swiss-Prot
Match: Q8BVE8 (Histone-lysine N-methyltransferase NSD2 OS=Mus musculus OX=10090 GN=Nsd2 PE=1 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 2.1e-11
Identity = 37/105 (35.24%), Postives = 46/105 (43.81%), Query Frame = 0

Query: 3    KKKCKTKEEIG------EDFCFTCKDGGLLRFCDFKNCLKAYHPECVGREDSFVESEDRW 62
            KKK + +   G      ED CF C DGG L  CD K C KAYH  C+G          +W
Sbjct: 1222 KKKTRRRRAKGEGKRQSEDECFRCGDGGQLVLCDRKFCTKAYHLSCLGLGK---RPFGKW 1281

Query: 63   TCDWHSCFLCHKTSKFRCVGCPQAVCGRCIFNAEFVRVRGSRGFC 102
             C WH C +C K S   C  CP + C        F   +  + +C
Sbjct: 1282 ECPWHHCDVCGKPSTSFCHLCPNSFCKEHQDGTAFRSTQDGQSYC 1323

BLAST of Lag0018861 vs. ExPASy Swiss-Prot
Match: Q6P2L6 (Histone-lysine N-methyltransferase NSD3 OS=Mus musculus OX=10090 GN=Nsd3 PE=1 SV=2)

HSP 1 Score: 68.9 bits (167), Expect = 4.7e-11
Identity = 34/83 (40.96%), Postives = 41/83 (49.40%), Query Frame = 0

Query: 3    KKKCKTKEE---IGEDFCFTCKDGGLLRFCDFKNCLKAYHPECVGREDSFVESEDRWTCD 62
            KK+ K K E   I ED+CF C DGG L  CD K+C KAYH  C+           +W C 
Sbjct: 1309 KKRRKVKAEAKPIHEDYCFQCGDGGELVMCDKKDCPKAYHLLCLNLTQ---PPHGKWECP 1368

Query: 63   WHSCFLCHKTSKFRCVGCPQAVC 83
            WH C  C   +   C  CP + C
Sbjct: 1369 WHRCDECGSVAVSFCEFCPHSFC 1388

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022984438.12.1e-6491.74uncharacterized protein At5g08430-like [Cucurbita maxima][more]
KAG7031352.11.0e-6391.74hypothetical protein SDJN02_05392, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023539109.11.0e-6391.74uncharacterized protein At5g08430-like [Cucurbita pepo subsp. pepo][more]
KAG6600713.11.0e-6391.74hypothetical protein SDJN03_05946, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022942556.11.0e-6391.74uncharacterized protein At5g08430-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SD347.3e-2845.61Zinc finger CCCH domain-containing protein 44 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9SIV51.1e-2342.34Zinc finger CCCH domain-containing protein 19 OS=Arabidopsis thaliana OX=3702 GN... [more]
O960282.1e-1136.19Histone-lysine N-methyltransferase NSD2 OS=Homo sapiens OX=9606 GN=NSD2 PE=1 SV=... [more]
Q8BVE82.1e-1135.24Histone-lysine N-methyltransferase NSD2 OS=Mus musculus OX=10090 GN=Nsd2 PE=1 SV... [more]
Q6P2L64.7e-1140.96Histone-lysine N-methyltransferase NSD3 OS=Mus musculus OX=10090 GN=Nsd3 PE=1 SV... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001965Zinc finger, PHD-typeSMARTSM00249PHD_3coord: 16..62
e-value: 1.3E-6
score: 37.9
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 2..108
e-value: 3.4E-20
score: 74.1
NoneNo IPR availablePANTHERPTHR46851:SF2GYF DOMAIN PROTEINcoord: 1..122
NoneNo IPR availablePANTHERPTHR46851OS01G0884500 PROTEINcoord: 1..122
IPR011011Zinc finger, FYVE/PHD-typeSUPERFAMILY57903FYVE/PHD zinc fingercoord: 13..70

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0018861.1Lag0018861.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003677 DNA binding
molecular_function GO:0046872 metal ion binding