Lag0018833 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0018833
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr5: 35165165 .. 35165713 (+)
RNA-Seq ExpressionLag0018833
SyntenyLag0018833
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCGATTAATTCAAACTAGGGATATGGTTTCTCAGATGTTTTCAGGAGGGGAGGACTTTATTTTACATGTTGATGCAGCGTGGACTAAACACTCCATGACCAGTGGTGTGGGGGTTGTGTTGCGCTCAAAGACGGGCAAATTGGTGGCTATTTTTCAAAGAGGAATCCCTTTAACTTCATCGCCATTATGTGCTGAGGCGATAGCGATTCTTGAAGGTCTCAAAATGCTTTTCTCCCTAAGGATTAGTAAGGTCACGGTATGTTCGGATTCCCTATCGTTAATTACTATTCTTCGGGATAAAGATCGGTGTCAAGCAGATTGCTTTCCAGTGGTGGCAGATATTCAAGGTCTGATTGGTTCTTTTGCCAAGATTTCTTTCTGTCATATAAAAATGGAGTATAATTTAATGTCACATGAGCTAGCAAGGTTAGGGATGGTGTCTTCTACTCAATATTGGAGTAGGAATTTCCCTCAATGGGTGTTAGAGTTGGCTAGTACAGAATTGTCTTTTTTTGTAACCCCTTGCGGGAGTCTTTGTTCCTGA

mRNA sequence

ATGGATCGATTAATTCAAACTAGGGATATGGTTTCTCAGATGTTTTCAGGAGGGGAGGACTTTATTTTACATGTTGATGCAGCGTGGACTAAACACTCCATGACCAGTGGTGTGGGGGTTGTGTTGCGCTCAAAGACGGGCAAATTGGTGGCTATTTTTCAAAGAGGAATCCCTTTAACTTCATCGCCATTATGTGCTGAGGCGATAGCGATTCTTGAAGGTCTCAAAATGCTTTTCTCCCTAAGGATTAGTAAGGTCACGGTATGTTCGGATTCCCTATCGTTAATTACTATTCTTCGGGATAAAGATCGGTGTCAAGCAGATTGCTTTCCAGTGGTGGCAGATATTCAAGGTCTGATTGGTTCTTTTGCCAAGATTTCTTTCTGTCATATAAAAATGGAGTATAATTTAATGTCACATGAGCTAGCAAGGTTAGGGATGGTGTCTTCTACTCAATATTGGAGTAGGAATTTCCCTCAATGGGTGTTAGAGTTGGCTAGTACAGAATTGTCTTTTTTTGTAACCCCTTGCGGGAGTCTTTGTTCCTGA

Coding sequence (CDS)

ATGGATCGATTAATTCAAACTAGGGATATGGTTTCTCAGATGTTTTCAGGAGGGGAGGACTTTATTTTACATGTTGATGCAGCGTGGACTAAACACTCCATGACCAGTGGTGTGGGGGTTGTGTTGCGCTCAAAGACGGGCAAATTGGTGGCTATTTTTCAAAGAGGAATCCCTTTAACTTCATCGCCATTATGTGCTGAGGCGATAGCGATTCTTGAAGGTCTCAAAATGCTTTTCTCCCTAAGGATTAGTAAGGTCACGGTATGTTCGGATTCCCTATCGTTAATTACTATTCTTCGGGATAAAGATCGGTGTCAAGCAGATTGCTTTCCAGTGGTGGCAGATATTCAAGGTCTGATTGGTTCTTTTGCCAAGATTTCTTTCTGTCATATAAAAATGGAGTATAATTTAATGTCACATGAGCTAGCAAGGTTAGGGATGGTGTCTTCTACTCAATATTGGAGTAGGAATTTCCCTCAATGGGTGTTAGAGTTGGCTAGTACAGAATTGTCTTTTTTTGTAACCCCTTGCGGGAGTCTTTGTTCCTGA

Protein sequence

MDRLIQTRDMVSQMFSGGEDFILHVDAAWTKHSMTSGVGVVLRSKTGKLVAIFQRGIPLTSSPLCAEAIAILEGLKMLFSLRISKVTVCSDSLSLITILRDKDRCQADCFPVVADIQGLIGSFAKISFCHIKMEYNLMSHELARLGMVSSTQYWSRNFPQWVLELASTELSFFVTPCGSLCS
Homology
BLAST of Lag0018833 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 78.6 bits (192), Expect = 6.3e-11
Identity = 53/149 (35.57%), Postives = 77/149 (51.68%), Query Frame = 0

Query: 23   LHVDAAWTKHSMTSGVGVVLRSKTGKLVAIFQRGIPLTSSPLCAEAIAILEGLKMLFSLR 82
            L+ DAA      ++  G ++R  +  LVA     +P   SPL AE   ILEGLK   +  
Sbjct: 1304 LNTDAAC--RGASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAEIRCILEGLKFAAASN 1363

Query: 83   ISKVTVCSDSLSLITILRDKDRCQADCFPVVADIQGLIGSFAKISFCHIKMEYNLMSHEL 142
             + + V SDSL  I ++R++   + D    V +IQ L   FA ISF H   + N  +H L
Sbjct: 1364 FTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALTCCFAFISFSHSSRQCNRAAHGL 1423

Query: 143  ARLGMV--SSTQYWSRNFPQWVLELASTE 170
            A+ G+   S+T  W  NFP W+L+L   +
Sbjct: 1424 AKWGITSPSATYAWLFNFPTWLLDLVQRD 1450

BLAST of Lag0018833 vs. NCBI nr
Match: RYR10083.1 (hypothetical protein Ahy_B05g078550 [Arachis hypogaea])

HSP 1 Score: 72.4 bits (176), Expect = 4.5e-09
Identity = 48/141 (34.04%), Postives = 73/141 (51.77%), Query Frame = 0

Query: 23  LHVDAAWTKHSMTSGVGVVLRSKTGKLVAIFQRGIPLTSSPLCAEAIAILEGLKMLFSLR 82
           ++VDAA   H +  GVGVV+R++ G ++A   + IP       AEA A   GL       
Sbjct: 180 VNVDAA-VPHDINGGVGVVIRNEMGIIMAAATQAIPYPLEEHEAEAYAAFWGLNFAKDCC 239

Query: 83  ISKVTVCSDSLSLITILRDKDRCQADCF-PVVADIQGLIGSFAKISFCHIKMEYNLMSHE 142
            SKV + SD++ ++  L+ + RC   CF   + D   LI +F  + F H+K E N ++HE
Sbjct: 240 FSKVILESDNIEVMGALKQR-RCFNSCFGTFITDAISLIRNFRFVEFSHVKREGNKVAHE 299

Query: 143 LARLGMVSSTQYWSRNFPQWV 163
           LA L + +    W  + P  V
Sbjct: 300 LATLALTTPNCMWMEDAPSHV 318

BLAST of Lag0018833 vs. NCBI nr
Match: KAF5447347.1 (hypothetical protein F2P56_032905 [Juglans regia])

HSP 1 Score: 71.6 bits (174), Expect = 7.7e-09
Identity = 39/126 (30.95%), Postives = 68/126 (53.97%), Query Frame = 0

Query: 26  DAAWTKHSMTSGVGVVLRSKTGKLVAIFQRGIPLTSSPLCAEAIAILEGLKMLFSLRISK 85
           DAA  K     G+G+++R+ TG +VA  +   PL   P  AEAI  LE  ++   L ++ 
Sbjct: 87  DAAVDKAKCKVGIGIIVRNHTGSVVATSRLNRPLYPDPALAEAIGALEAARLCHHLGLTN 146

Query: 86  VTVCSDSLSLITILRDKDRCQADCFPVVADIQGLIGSFAKISFCHIKMEYNLMSHELARL 145
           + +  D+L +I  +  +    +    ++ D++G + +F   S  HI+ E N  +HELA+ 
Sbjct: 147 IILEGDALQVIQAINSRSENWSSTGMIMEDVKGQLSTFESWSAIHIRREGNKAAHELAKN 206

Query: 146 GMVSST 152
            +V+ST
Sbjct: 207 ALVTST 212

BLAST of Lag0018833 vs. NCBI nr
Match: XP_035541483.1 (uncharacterized protein LOC118344571 [Juglans regia])

HSP 1 Score: 71.6 bits (174), Expect = 7.7e-09
Identity = 39/126 (30.95%), Postives = 68/126 (53.97%), Query Frame = 0

Query: 26  DAAWTKHSMTSGVGVVLRSKTGKLVAIFQRGIPLTSSPLCAEAIAILEGLKMLFSLRISK 85
           DAA  K     G+G+++R+ TG +VA  +   PL   P  AEAI  LE  ++   L ++ 
Sbjct: 70  DAAVDKAKCKVGIGIIVRNHTGSVVATSRLNRPLYPDPALAEAIGALEAARLCHHLGLTN 129

Query: 86  VTVCSDSLSLITILRDKDRCQADCFPVVADIQGLIGSFAKISFCHIKMEYNLMSHELARL 145
           + +  D+L +I  +  +    +    ++ D++G + +F   S  HI+ E N  +HELA+ 
Sbjct: 130 IILEGDALQVIQAINSRSENWSSTGMIMEDVKGQLSTFESWSAIHIRREGNKAAHELAKN 189

Query: 146 GMVSST 152
            +V+ST
Sbjct: 190 ALVTST 195

BLAST of Lag0018833 vs. NCBI nr
Match: XP_016199994.1 (uncharacterized protein LOC107641002 [Arachis ipaensis] >XP_025652971.1 uncharacterized protein LOC112748935 [Arachis hypogaea])

HSP 1 Score: 71.2 bits (173), Expect = 1.0e-08
Identity = 48/143 (33.57%), Postives = 72/143 (50.35%), Query Frame = 0

Query: 25  VDAAWTKHSMTSGVGVVLRSKTGKLVAIFQRGIPLTSSPLCAEAIAILEGLKMLFSLRIS 84
           VDAA   H +  GVGVV+R + G ++A+  + IP       AEA A   GL  +     +
Sbjct: 28  VDAA-VPHDINGGVGVVIRDEMGIIMALAIQAIPYPFEAHEAEAYAAFWGLNFIKDCCFA 87

Query: 85  KVTVCSDSLSLITILRDKDRCQADCF-PVVADIQGLIGSFAKISFCHIKMEYNLMSHELA 144
           KV + SD+  ++  L  K RC + CF   + D   LI +F  + F H+K E N ++HELA
Sbjct: 88  KVILKSDNTEVMGAL--KQRCFSSCFGTFIIDAISLIKNFRSVEFSHVKREGNRVAHELA 147

Query: 145 RLGMVSSTQYWSRNFPQWVLELA 167
            L + +    W  +    +  LA
Sbjct: 148 TLALTNPNCMWMEDVLNHIQNLA 167

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158377.16.3e-1135.57uncharacterized protein LOC111024874 [Momordica charantia][more]
RYR10083.14.5e-0934.04hypothetical protein Ahy_B05g078550 [Arachis hypogaea][more]
KAF5447347.17.7e-0930.95hypothetical protein F2P56_032905 [Juglans regia][more]
XP_035541483.17.7e-0930.95uncharacterized protein LOC118344571 [Juglans regia][more]
XP_016199994.11.0e-0833.57uncharacterized protein LOC107641002 [Arachis ipaensis] >XP_025652971.1 uncharac... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 20..148
e-value: 3.3E-16
score: 61.5
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 25..145
e-value: 5.0E-21
score: 74.8
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 23..169
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 23..169
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 23..144
e-value: 4.8598E-21
score: 81.5916
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 21..148

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0018833.1Lag0018833.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity