Lag0018774 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0018774
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr5: 34182897 .. 34185230 (+)
RNA-Seq ExpressionLag0018774
SyntenyLag0018774
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTTTAGCTCCAATTGGATCCAGAAGGTTATGAGTTGCATCTCCTCTGTATGCTACTTAGTTCTTGTTAATGGCGAGCACCAAACAGAGTTTAAGCCGAGCAGAGGCCTCCGGCAGGGAGACCCCCTGTCGCCCTACCTGTTCCTGTTGTGTGCGGAAGGCTTTTCCGCTCTTTTGGAAAGGAAAGAATCTCTTTCTAAAATTTCTGGTTTTAAAATTAACAAGCTTTGTCCTTCTCTAACTCATCTCTTCTTTGCAGATGACAGCCTAGTCTTTCTTAAAGCAAAATACTCGGATCTTCGAGCCTTCAAACAAGTTTTGAAACACTACGAGGGAGCGTCGGGCCAGACTATCAACCTTGACAAGTCCACCTTTATGGCTAGTAAAAACGTTAAGGATGAGACTTTGGCCAAGTGTGAAGAAGTTCTAGGTATCAAGAGATCGAGTTCGTTAGGCCAATATTTGGGGATGCCTTCGCAAACAGGGAGAAATAAAGGAGTGGTGTTCAAAAGAATCAAAGACAGGGTTGAGAAAACTCTTCAAGGATGGAAAGGAAATCTCTTTTCCATGGGAGGAAAGGAAGTGCTTATTAAGGCTGTGGCTCAGGCGATCCCAGTCTATACCATGAGTTGTTTTCGACTACCCACTAATATCTGTTCTTTTATTGACAGGTTATGCGCTAAATTGTGGTGGGGCTCGGCTGGGAATAAAGACAAAACTCACTGGATAAGCTGGAAGAAGATGTGCAAAAACAAGAGTCCTAAACAGAGCTGGAGGCTGATAAGGAACCCCAATAGCCTCCTGTTCAAGATTCTGAGAGGGCGATATTTCAAAGGAAGTAATTTCCTTGAAGCTCCCATTGGCAACTCTCCTTCCCTCACTTGGTGAAGCATAGTTTGGGGCAGGGACCTGTTCCTTAAGGGTTACCGCTGGAGAGTTGGAAACGGAGCCCAAATTTTCATTGACAAGGATCCTTGGATAAGCCGAGAAGGCAATCCTAGAGTCATCACCCTGTCCAACTCCTATGTGGGCCTTAGAGTCAAAGATTTCCTTGATGACAACAACAAGTGGAAAGAAAATGCAATTAGGGAAGCTTTCTCTCCTCAAGATGCGTCCGATATCCTGAATATGCATGCAGGGAGTAAGGATTCAAAGGACGAAATTGTGTGGAGCTTCGACAAAAAAGGCATTTTCTCTGTGAAAAGTGCCTACCGACTAGCCTCGAAAGGCCTTAGCACTTATGAAGCCTCCCATTCTAACCAATCTAAGGAAGCAGCTTTCTGGAACAGTATTTGGAAGGCCAATGTTCTCCCTAGATCCAAAGTGTGTGCGTGGAGGATTATCAATGACATCATCCCTACAAAGGCTAATGCTCTAAAAAAGGGAGTTGATCTTATTCCCTTATGCTCTTTTTGTGGAAAATATAAAGAATCTTCTTCCCATCTAATATGGGAATGCAAAATGGTTAAATCTATATGGTCCTATTTTGTTCCAAGCTCTCTTACTATGTGGTCTTTGTGTAGGGAGGATTGGAAGCCTAAGGACTACTGGGGATGGATGGAAGCGAATCTAAACAGGGAAGACATAGACAGAAGTATAATCATAATGTAGCAAATTTGGGATTTCAGAAACAAATCAGAACATTACAACATCCCAACAACAACAAAATTTCTCCAGAATTCCATCGAAGTTAAATTAAAGGAATGAGAATCTTCTTACCTCAAGACCCATTACCCGAAAAGGCTGAGGACTCCGGCGAGTCAAGCTCAGTGGGAGAAGCCACGGCCGAACACGTGGAAGCTCAACTCAGACGCAACCTGGATGGAAAAAGAGGGTTGCAGAGGTCTCGGATGGCTCGTGCGTGACTCGGAGGGTTCCTTGATCTGTTTCGGGATGAAGCAAGTTAAACAAAATTGGGCTATAAAGAATCTGGAAGCTTGCGCTATGTTGGAAGGTATCAAAGAAGTTTCAGATACCTGTAATCGCCTCGGAATTCATCTGGAAGTCGAGACGGACGCCAACGAGGTCGTTCGGGTTCTCAACGGCGAGGAGGAAGATTTATCAGACCTGAAGACTTTCACCGATGAAATCAAGGCACTAGCTGACCGTGCCTTCTCCATGAAATTCAGCCATTGTAATCGCCTTTTGAACACAGCTGCACACTGTGTTGCGAGGAGCGCTGCCAGCAAGTTCTCGCCGTCTCCGGCGATGTCTTCTGGCGCTTTGTCTTCTTCGCGGGAACGGGAAGTATGTTTTTCGGCTCCCAACATTCCGGTTTGGGCTTTCCCTCTAATTAATGAGGGTGGTTGTACTAGACTGCTTTTGTCTTAA

mRNA sequence

ATGGGTTTTAGCTCCAATTGGATCCAGAAGGTTATGAGTTGCATCTCCTCTGTATGCTACTTAGTTCTTGTTAATGGCGAGCACCAAACAGAGTTTAAGCCGAGCAGAGGCCTCCGGCAGGGAGACCCCCTGTCGCCCTACCTGTTCCTGTTGTGTGCGGAAGGCTTTTCCGCTCTTTTGGAAAGGAAAGAATCTCTTTCTAAAATTTCTGGTTTTAAAATTAACAAGCTTTGTCCTTCTCTAACTCATCTCTTCTTTGCAGATGACAGCCTAGTCTTTCTTAAAGCAAAATACTCGGATCTTCGAGCCTTCAAACAAGTTTTGAAACACTACGAGGGAGCGTCGGGCCAGACTATCAACCTTGACAAGTCCACCTTTATGGCTAGTAAAAACGTTAAGGATGAGACTTTGGCCAAGTGTGAAGAAGTTCTAGGTATCAAGAGATCGAGTTCGTTAGGCCAATATTTGGGGATGCCTTCGCAAACAGGGAGAAATAAAGGAGTGGTGTTCAAAAGAATCAAAGACAGGGTTGAGAAAACTCTTCAAGGATGGAAAGGAAATCTCTTTTCCATGGGAGGAAAGGAAGTGCTTATTAAGGCTGTGGCTCAGGCGATCCCAGTCTATACCATGAGTTGTTTTCGACTACCCACTAATATCTGTTCTTTTATTGACAGGTTATGCGCTAAATTGTGGTGGGGCTCGGCTGGGAATAAAGACAAAACTCACTGGATAAGCTGGAAGAAGATGTGCAAAAACAAGAGTCCTAAACAGAGCTGGAGGCTGATAAGGAACCCCAATAGCCTCCTGTTCAAGATTCTGAGAGGGCGATATTTCAAAGGAAGTAATTTCCTTGAAGCTCCCATTGGCAACTCTCCTTCCCTCACTTGCCGAGAAGGCAATCCTAGAGTCATCACCCTGTCCAACTCCTATGTGGGCCTTAGAGTCAAAGATTTCCTTGATGACAACAACAAGTGGAAAGAAAATGCAATTAGGGAAGCTTTCTCTCCTCAAGATGCGTCCGATATCCTGAATATGCATGCAGGGAGTAAGGATTCAAAGGACGAAATTGTGTGGAGCTTCGACAAAAAAGGCATTTTCTCTGTGAAAAGTGCCTACCGACTAGCCTCGAAAGGCCTTAGCACTTATGAAGCCTCCCATTCTAACCAATCTAAGGAAGCAGCTTTCTGGAACAGTATTTGGAAGGCCAATGTTCTCCCTAGATCCAAAGTGTGTGCGTGGAGGATTATCAATGACATCATCCCTACAAAGGCTAATGCTCTAAAAAAGGGAGTTGATCTTATTCCCTTATGCTCTTTTTGTGGAAAATATAAAGAATCTTCTTCCCATCTAATATGGGAATGCAAAATGGTTAAATCTATATGGTCCTATTTTGTTCCAAGCTCTCTTACTATGTGGTCTTTGTGTAGGGAGGATTGGAAGCCTAAGGACTACTGGGGATGGATGGAAGCGAATCTAAACAGGGAAGACATAGACAGAAGTATAATCATAATGCTGAGGACTCCGGCGAGTCAAGCTCAGTGGGAGAAGCCACGGCCGAACACGTGGAAGCTCAACTCAGACGCAACCTGGATGGAAAAAGAGGGTTGCAGAGGTCTCGGATGGCTCGTGCGTGACTCGGAGGGTTCCTTGATCTGTTTCGGGATGAAGCAAGTTAAACAAAATTGGGCTATAAAGAATCTGGAAGCTTGCGCTATGTTGGAAGGTATCAAAGAAGTTTCAGATACCTGTAATCGCCTCGGAATTCATCTGGAAGTCGAGACGGACGCCAACGAGGTCGTTCGGGTTCTCAACGGCGAGGAGGAAGATTTATCAGACCTGAAGACTTTCACCGATGAAATCAAGGCACTAGCTGACCGTGCCTTCTCCATGAAATTCAGCCATTGTAATCGCCTTTTGAACACAGCTGCACACTGTGTTGCGAGGAGCGCTGCCAGCAAGTTCTCGCCGTCTCCGGCGATGTCTTCTGGCGCTTTGTCTTCTTCGCGGGAACGGGAAGTATGTTTTTCGGCTCCCAACATTCCGGTTTGGGCTTTCCCTCTAATTAATGAGGGTGGTTGTACTAGACTGCTTTTGTCTTAA

Coding sequence (CDS)

ATGGGTTTTAGCTCCAATTGGATCCAGAAGGTTATGAGTTGCATCTCCTCTGTATGCTACTTAGTTCTTGTTAATGGCGAGCACCAAACAGAGTTTAAGCCGAGCAGAGGCCTCCGGCAGGGAGACCCCCTGTCGCCCTACCTGTTCCTGTTGTGTGCGGAAGGCTTTTCCGCTCTTTTGGAAAGGAAAGAATCTCTTTCTAAAATTTCTGGTTTTAAAATTAACAAGCTTTGTCCTTCTCTAACTCATCTCTTCTTTGCAGATGACAGCCTAGTCTTTCTTAAAGCAAAATACTCGGATCTTCGAGCCTTCAAACAAGTTTTGAAACACTACGAGGGAGCGTCGGGCCAGACTATCAACCTTGACAAGTCCACCTTTATGGCTAGTAAAAACGTTAAGGATGAGACTTTGGCCAAGTGTGAAGAAGTTCTAGGTATCAAGAGATCGAGTTCGTTAGGCCAATATTTGGGGATGCCTTCGCAAACAGGGAGAAATAAAGGAGTGGTGTTCAAAAGAATCAAAGACAGGGTTGAGAAAACTCTTCAAGGATGGAAAGGAAATCTCTTTTCCATGGGAGGAAAGGAAGTGCTTATTAAGGCTGTGGCTCAGGCGATCCCAGTCTATACCATGAGTTGTTTTCGACTACCCACTAATATCTGTTCTTTTATTGACAGGTTATGCGCTAAATTGTGGTGGGGCTCGGCTGGGAATAAAGACAAAACTCACTGGATAAGCTGGAAGAAGATGTGCAAAAACAAGAGTCCTAAACAGAGCTGGAGGCTGATAAGGAACCCCAATAGCCTCCTGTTCAAGATTCTGAGAGGGCGATATTTCAAAGGAAGTAATTTCCTTGAAGCTCCCATTGGCAACTCTCCTTCCCTCACTTGCCGAGAAGGCAATCCTAGAGTCATCACCCTGTCCAACTCCTATGTGGGCCTTAGAGTCAAAGATTTCCTTGATGACAACAACAAGTGGAAAGAAAATGCAATTAGGGAAGCTTTCTCTCCTCAAGATGCGTCCGATATCCTGAATATGCATGCAGGGAGTAAGGATTCAAAGGACGAAATTGTGTGGAGCTTCGACAAAAAAGGCATTTTCTCTGTGAAAAGTGCCTACCGACTAGCCTCGAAAGGCCTTAGCACTTATGAAGCCTCCCATTCTAACCAATCTAAGGAAGCAGCTTTCTGGAACAGTATTTGGAAGGCCAATGTTCTCCCTAGATCCAAAGTGTGTGCGTGGAGGATTATCAATGACATCATCCCTACAAAGGCTAATGCTCTAAAAAAGGGAGTTGATCTTATTCCCTTATGCTCTTTTTGTGGAAAATATAAAGAATCTTCTTCCCATCTAATATGGGAATGCAAAATGGTTAAATCTATATGGTCCTATTTTGTTCCAAGCTCTCTTACTATGTGGTCTTTGTGTAGGGAGGATTGGAAGCCTAAGGACTACTGGGGATGGATGGAAGCGAATCTAAACAGGGAAGACATAGACAGAAGTATAATCATAATGCTGAGGACTCCGGCGAGTCAAGCTCAGTGGGAGAAGCCACGGCCGAACACGTGGAAGCTCAACTCAGACGCAACCTGGATGGAAAAAGAGGGTTGCAGAGGTCTCGGATGGCTCGTGCGTGACTCGGAGGGTTCCTTGATCTGTTTCGGGATGAAGCAAGTTAAACAAAATTGGGCTATAAAGAATCTGGAAGCTTGCGCTATGTTGGAAGGTATCAAAGAAGTTTCAGATACCTGTAATCGCCTCGGAATTCATCTGGAAGTCGAGACGGACGCCAACGAGGTCGTTCGGGTTCTCAACGGCGAGGAGGAAGATTTATCAGACCTGAAGACTTTCACCGATGAAATCAAGGCACTAGCTGACCGTGCCTTCTCCATGAAATTCAGCCATTGTAATCGCCTTTTGAACACAGCTGCACACTGTGTTGCGAGGAGCGCTGCCAGCAAGTTCTCGCCGTCTCCGGCGATGTCTTCTGGCGCTTTGTCTTCTTCGCGGGAACGGGAAGTATGTTTTTCGGCTCCCAACATTCCGGTTTGGGCTTTCCCTCTAATTAATGAGGGTGGTTGTACTAGACTGCTTTTGTCTTAA

Protein sequence

MGFSSNWIQKVMSCISSVCYLVLVNGEHQTEFKPSRGLRQGDPLSPYLFLLCAEGFSALLERKESLSKISGFKINKLCPSLTHLFFADDSLVFLKAKYSDLRAFKQVLKHYEGASGQTINLDKSTFMASKNVKDETLAKCEEVLGIKRSSSLGQYLGMPSQTGRNKGVVFKRIKDRVEKTLQGWKGNLFSMGGKEVLIKAVAQAIPVYTMSCFRLPTNICSFIDRLCAKLWWGSAGNKDKTHWISWKKMCKNKSPKQSWRLIRNPNSLLFKILRGRYFKGSNFLEAPIGNSPSLTCREGNPRVITLSNSYVGLRVKDFLDDNNKWKENAIREAFSPQDASDILNMHAGSKDSKDEIVWSFDKKGIFSVKSAYRLASKGLSTYEASHSNQSKEAAFWNSIWKANVLPRSKVCAWRIINDIIPTKANALKKGVDLIPLCSFCGKYKESSSHLIWECKMVKSIWSYFVPSSLTMWSLCREDWKPKDYWGWMEANLNREDIDRSIIIMLRTPASQAQWEKPRPNTWKLNSDATWMEKEGCRGLGWLVRDSEGSLICFGMKQVKQNWAIKNLEACAMLEGIKEVSDTCNRLGIHLEVETDANEVVRVLNGEEEDLSDLKTFTDEIKALADRAFSMKFSHCNRLLNTAAHCVARSAASKFSPSPAMSSGALSSSREREVCFSAPNIPVWAFPLINEGGCTRLLLS
Homology
BLAST of Lag0018774 vs. NCBI nr
Match: XP_023881891.1 (uncharacterized protein LOC111994244 [Quercus suber])

HSP 1 Score: 411.8 bits (1057), Expect = 1.2e-110
Identity = 249/745 (33.42%), Postives = 376/745 (50.47%), Query Frame = 0

Query: 1    MGFSSNWIQKVMSCISSVCYLVLVNGEHQTEFKPSRGLRQGDPLSPYLFLLCAEGFSALL 60
            MGF   WI+ VM CI+SV Y +LVNG       P+RGLRQGDP+SPY+FLLCA+GFS+LL
Sbjct: 436  MGFHEKWIKLVMHCITSVSYSILVNGGAYGSITPTRGLRQGDPISPYIFLLCADGFSSLL 495

Query: 61   ERKESLSKISGFKINKLCPSLTHLFFADDSLVFLKAKYSDLRAFKQVLKHYEGASGQTIN 120
                   +ISG  I + CP +THLFFADDSL+F KA   + +    +L+ YE ASGQ IN
Sbjct: 496  NDVARKLRISGVSICRGCPKITHLFFADDSLLFCKANSQECQTLIDILQLYEDASGQKIN 555

Query: 121  LDKSTFMASKNVKDETLAKCEEVLGIKRSSSLGQYLGMPSQTGRNKGVVFKRIKDRVEKT 180
            +DKS+   S N  DE   +   +LG  + +   +YLG+PS  G++K  +F  +K+RVE+ 
Sbjct: 556  VDKSSVFFSNNTPDEKRCEVLRMLGHMQDTRHKKYLGLPSIIGKSKVEIFAEVKERVERK 615

Query: 181  LQGWKGNLFSMGGKEVLIKAVAQAIPVYTMSCFRLPTNICSFIDRLCAKLWWGSAGNKDK 240
            L GWK  L S+GG+E+LIKAVAQAIP YTMSCF++P  +C  I+ +  + WWG  G + K
Sbjct: 616  LSGWKEKLLSVGGREILIKAVAQAIPTYTMSCFQIPKTLCEEIEAMMRRFWWGQRGQESK 675

Query: 241  THWISWKKMCKNKS-----------------PKQSWRLIRNPNSLLFKILRGRYFKGSNF 300
              W+SWKK+CK K                   KQ WRLI NPNSL+ +I + RY+   + 
Sbjct: 676  IAWVSWKKLCKAKKNGGMGFRNLQAFNLAMLAKQGWRLISNPNSLVAQIYKARYYPHGDV 735

Query: 301  LEAPIGNSPSLTCRE----------------GN-PRVITLSNSYVGL------------- 360
             +A +G SPS T R                 GN  R++   + ++               
Sbjct: 736  FQAKLGASPSYTWRSIFNGLEVVRRGTRWRVGNGERILIWEDKWLPTPITYKVISPPKPF 795

Query: 361  ----RVKDFLD-DNNKWKENAIREAFSPQDASDILNMHAGSKDSKDEIVWSFDKKGIFSV 420
                RV   +D +  +WK++ +R+ F P +A  IL++       +D+I+W  ++KG FSV
Sbjct: 796  DDYPRVSALIDRERKRWKDDVVRDLFLPFEARTILSIPLSHNFPEDQIIWVGNRKGEFSV 855

Query: 421  KSAYRLASKGLSTYEASHSNQ-SKEAAFWNSIWKANVLPRSKVCAWRIINDIIPTKANAL 480
            KSAY +A   +   E   S+     +  W  +W  N+ P+ ++ AW++  + +PT  N L
Sbjct: 856  KSAYYIAVGVIDNLEVGESSSGDSRSLLWRKLWHLNIPPKVRIFAWKMCMNALPTFLNLL 915

Query: 481  KKGVDLIPLCSFCGKYKESSSHLIWECKMVKSIW-------------------------S 540
            +KGV++  +C  CG   ES+ H+  +C++ K +W                          
Sbjct: 916  RKGVNICDVCPACGMEPESNIHIFVKCEVAKRVWRCWLDNPADLVNVNMDIVDIAMKILD 975

Query: 541  YFVPSSLTM-----WSL-CRED--------WKPKDYWGWMEANLNREDIDRSIIIMLRTP 600
            +  PS L +     W++ C  +          P+  WG+    +  E  + S       P
Sbjct: 976  FGTPSDLEIFFVVAWAIWCNRNKIVFESLSQVPEFIWGFAIKYI-LEFKNASATYCQILP 1035

Query: 601  ASQAQWEKPRPNTWKLNSDATWMEKEGCRGLGWLVRDSEGSLICFGMKQVKQNWAIKNLE 654
             S  +W  P P  +K+N D    E      +G ++RD+ G +       ++  ++++ +E
Sbjct: 1036 QSDGKWMAPPPGVFKINVDGATSEYGRNSSVGVIIRDATGDVCAACCTYLQGQYSVEEVE 1095

BLAST of Lag0018774 vs. NCBI nr
Match: XP_023878301.1 (uncharacterized protein LOC111990748 [Quercus suber])

HSP 1 Score: 409.8 bits (1052), Expect = 4.6e-110
Identity = 251/741 (33.87%), Postives = 362/741 (48.85%), Query Frame = 0

Query: 1    MGFSSNWIQKVMSCISSVCYLVLVNGEHQTEFKPSRGLRQGDPLSPYLFLLCAEGFSALL 60
            MGF + W   VM CI+SV Y +L+NG       PSRGLRQGDPLSP LFLLCAEG SAL+
Sbjct: 560  MGFCNRWRDLVMQCITSVSYSILINGVAHGNIYPSRGLRQGDPLSPSLFLLCAEGLSALI 619

Query: 61   ERKESLSKISGFKINKLCPSLTHLFFADDSLVFLKAKYSDLRAFKQVLKHYEGASGQTIN 120
             +      I+G  IN+ CP +THLFFADDS++F KA Y +    + +L  YE ASGQ IN
Sbjct: 620  NQAARNKLITGISINRGCPKVTHLFFADDSILFCKAAYEECHLLRSILGQYEEASGQKIN 679

Query: 121  LDKSTFMASKNVKDETLAKCEEVLGIKRSSSLGQYLGMPSQTGRNKGVVFKRIKDRVEKT 180
             DKS+   S N   ET  +   +LG  ++S   +YLG+PS  GR+K  VF  +K++V   
Sbjct: 680  TDKSSIFFSPNTAQETRDEIFNILGPMQNSRHTKYLGLPSLIGRSKSQVFAMLKEKVGHK 739

Query: 181  LQGWKGNLFSMGGKEVLIKAVAQAIPVYTMSCFRLPTNICSFIDRLCAKLWWGSAGNKDK 240
            L GWKG L SMGGKE+LIKAVAQAIP YTMSCF LP  +C  ++R+    WWG    + K
Sbjct: 740  LAGWKGKLLSMGGKEILIKAVAQAIPTYTMSCFLLPQGLCDDMERMMKNFWWGQRNQETK 799

Query: 241  THWISWKKMCKNKS-----------------PKQSWRLIRNPNSLLFKILRGRYFKGSNF 300
              WISWK+MC +K+                  KQ+WR++ NPNSL+ ++L+ RYF   + 
Sbjct: 800  MGWISWKRMCNSKASGGLGFRNLKAFNLAMLAKQAWRILYNPNSLVGRVLKARYFPTGDL 859

Query: 301  LEAPIGNSPSLTCRE----------------GNPRVITL------------------SNS 360
            L A +G+SPS + R                 GN + I +                   ++
Sbjct: 860  LNAKLGSSPSYSWRSIHSSLEVIRRGTRWRVGNGKQIHIWEDRWLPTPSTYKVISPQIHN 919

Query: 361  YVGLRVKDFLDDNNK-WKENAIREAFSPQDASDILNMHAGSKDSKDEIVWSFDKKGIFSV 420
            +    V   +D + K WK  A+R  F P +   IL +       +D+++W  +KKG FSV
Sbjct: 920  FEFPLVSSLIDPDTKWWKVEALRSIFLPFEVETILRIPLSYNLPEDKLIWIGNKKGEFSV 979

Query: 421  KSAYRLASKGLSTYEASH-SNQSKEAAFWNSIWKANVLPRSKVCAWRIINDIIPTKANAL 480
            KSAY +A   +   E    SN       W  +W  N+  + K+ AWR   D +PT  N  
Sbjct: 980  KSAYHIAHSIIDPNERGECSNGDPYRLLWKKLWLLNLPGKIKIFAWRACVDGLPTYDNIS 1039

Query: 481  KKGVDLIPLCSFCGKYKESSSHLIWECKMVKSIWSY------------------------ 540
            K+G+     C  CG   E  +H +  C+    +W +                        
Sbjct: 1040 KRGICCSSTCPICGLVTEDVNHALLYCEAASLVWCFWSDYPETPQSHNGSFLDMALHLCH 1099

Query: 541  ---------FVPSSLTMW----SLCREDWKPKDYWGWMEANLNREDIDRSIIIMLRTPA- 600
                     F   S  +W     +   D        W+ AN   ED  ++  + +  P  
Sbjct: 1100 SKASQVLELFFVLSWAIWYNRNKIVHNDSPLSPSQVWLMANNTLEDFKKAASLDIIPPRH 1159

Query: 601  SQAQWEKPRPNTWKLNSDATWMEKEGCRGLGWLVRDSEGSLICFGMKQVKQNWAIKNLEA 651
            SQ +WE P    +K+N D    ++     +G ++RDS G ++    K +   +A   +EA
Sbjct: 1160 SQIRWEAPPLGIFKVNVDGATSDQGRNSSIGVIIRDSNGLVVAALNKYLPGRFAADQVEA 1219

BLAST of Lag0018774 vs. NCBI nr
Match: XP_030946812.1 (uncharacterized protein LOC115971195 [Quercus lobata])

HSP 1 Score: 396.7 bits (1018), Expect = 4.0e-106
Identity = 242/748 (32.35%), Postives = 361/748 (48.26%), Query Frame = 0

Query: 1    MGFSSNWIQKVMSCISSVCYLVLVNGEHQTEFKPSRGLRQGDPLSPYLFLLCAEGFSALL 60
            +GF + WI  V SCI SV + VLVNGE    F P+RGLRQGDPLSPYLFLLCAEG  +L+
Sbjct: 604  LGFDNRWITLVSSCIRSVSFSVLVNGEPHGNFTPNRGLRQGDPLSPYLFLLCAEGLHSLI 663

Query: 61   ERKESLSKISGFKINKLCPSLTHLFFADDSLVFLKAKYSDLRAFKQVLKHYEGASGQTIN 120
            ++ E    I G  +    P ++HLFFADDSL+F +A   ++ +  ++LK YE ASGQ IN
Sbjct: 664  QQVEISGSIKGVSLCSTVPKVSHLFFADDSLLFCRANSQEVSSIMEILKQYEEASGQQIN 723

Query: 121  LDKSTFMASKNVKDETLAKCEEVLGIKRSSSLGQYLGMPSQTGRNKGVVFKRIKDRVEKT 180
             +K+    S N       + + +LG+  +++  +YLG+PS  GR K   F  I++RV + 
Sbjct: 724  REKTQLFFSPNTDPHVQEEIKTLLGVAATTNYEKYLGLPSFVGRGKKQSFAYIRERVWQK 783

Query: 181  LQGWKGNLFSMGGKEVLIKAVAQAIPVYTMSCFRLPTNICSFIDRLCAKLWWGSAGNKDK 240
            +QGWK  L S GG+EVLIKAV QA+P +TM CF+LP ++C  I+ L  K WWG  G   K
Sbjct: 784  MQGWKERLLSQGGREVLIKAVLQAMPTFTMGCFKLPKSLCKDIESLIRKFWWGYKGEARK 843

Query: 241  THWISWKKMCKNKS-----------------PKQSWRLIRNPNSLLFKILRGRYFKGSNF 300
             HW+ WKK+CK+KS                  KQ WRLI N +SL +K+ + +YF   + 
Sbjct: 844  IHWVGWKKLCKSKSRGGLGFKDIELFNIAMLGKQVWRLIHNKDSLFYKVFKAKYFPNCSI 903

Query: 301  LEA--------------------------PIGNSPSLTCREG-------NPRVITLSNSY 360
            L+                            IG+  S+  R         + RV++   ++
Sbjct: 904  LDEGVKENGSYAWQSILKARGVVRMGSKWRIGDGHSVRIRGDKWLPDLFSSRVVSPQKNF 963

Query: 361  V-GLRVKDFLDDNNK-WKENAIREAFSPQDASDILNMHAGSKDSKDEIVWSFDKKGIFSV 420
                RV   +D+ N+ W E+ IRE F P +A  IL++       +D ++W+    G ++ 
Sbjct: 964  PNNTRVCALIDEENRCWMEDRIREEFLPHEAEAILSLPLSFNGKEDRLIWAETANGYYTT 1023

Query: 421  KSAYRLASKGLSTYEASHSNQSKEAAFWNSIWKANVLPRSKVCAWRIINDIIPTKANALK 480
            KSAYRL  +       S SN + E  FW  +W  NV  + +   WR  ND +P K N  K
Sbjct: 1024 KSAYRLLLQAAEAATPSSSNSAAEKPFWQELWSLNVPNKIRHFLWRAANDSLPIKRNLQK 1083

Query: 481  KGVDLIPLCSFCGKYKESSSHLIWECKMVKSIW------------SYFVPSSLTMWSLCR 540
            + +    +C  CG   E   H +W C+M+K +W            ++     L    L +
Sbjct: 1084 RNIIQDTICERCGDGIEDGIHALWGCQMIKQVWWELEKCKDFLNENFASFHDLLQGILAQ 1143

Query: 541  EDWKPKDYWG------WMEANLNR--------EDIDRSIIIMLR-------------TPA 600
            ++    + +G      W + N  R        E I R  +  LR             T  
Sbjct: 1144 KNPNVAELFGFIGWSIWHDRNARRLGSHSLPIEKIYRDAVERLREFHSVREEQRTQLTVH 1203

Query: 601  SQAQWEKPRPNTWKLNSDATWMEKEGCRGLGWLVRDSEGSLICFGMKQVKQNWAIKNLEA 657
                W    P+ +K+N D          GLG +VRDSEG +I    +++     +  LEA
Sbjct: 1204 HPTHWLPSSPSVYKVNFDGATFPDIVAAGLGVVVRDSEGLVIAALSERIHLPPTVTALEA 1263

BLAST of Lag0018774 vs. NCBI nr
Match: XP_023901742.1 (uncharacterized protein LOC112013579 [Quercus suber])

HSP 1 Score: 394.0 bits (1011), Expect = 2.6e-105
Identity = 245/746 (32.84%), Postives = 358/746 (47.99%), Query Frame = 0

Query: 1    MGFSSNWIQKVMSCISSVCYLVLVNGEHQTEFKPSRGLRQGDPLSPYLFLLCAEGFSALL 60
            +GF   WI  +M+C+SSV Y VL+NGE      PSRG+RQGDPLSP LFLLCAEG SAL+
Sbjct: 445  LGFMEKWIHLIMNCVSSVSYSVLINGEAYGNITPSRGIRQGDPLSPCLFLLCAEGLSALI 504

Query: 61   ERKESLSKISGFKINKLCPSLTHLFFADDSLVFLKAKYSDLRAFKQVLKHYEGASGQTIN 120
                   +I+G  I + CP +THLFFADDSL+F KAK  +  A   +L  YE ASGQ IN
Sbjct: 505  HEAARNQQINGISICRGCPLITHLFFADDSLLFCKAKEQECHALVSILNRYEEASGQKIN 564

Query: 121  LDKSTFMASKNVKDETLAKCEEVLGIKRSSSLGQYLGMPSQTGRNKGVVFKRIKDRVEKT 180
             DKS+   S N   E       +LG  + S   +YLG+PS  G++K  VF  +KDRV K 
Sbjct: 565  TDKSSVFFSPNTSQELRESIFNILGPMQDSRHSKYLGLPSIIGKSKAQVFAEVKDRVAKK 624

Query: 181  LQGWKGNLFSMGGKEVLIKAVAQAIPVYTMSCFRLPTNICSFIDRLCAKLWWGSAGNKDK 240
            L GWKG L S+GG+E+LIKAVAQA+P YTMSCF+LP  +C  ++ L    WWG    ++K
Sbjct: 625  LAGWKGKLLSIGGREILIKAVAQAVPTYTMSCFQLPKTLCQDLESLMRSFWWGQKDKENK 684

Query: 241  THWISWKKMCKNK-----------------SPKQSWRLIRNPNSLLFKILRGRYFKGSNF 300
              W+SW+KMC++K                   KQ WR++ NPNSL+ ++ + +YF   + 
Sbjct: 685  IAWVSWRKMCRSKLHGGMGFRNIQAFNLAMLAKQGWRILTNPNSLMARVFKAKYFPFDDV 744

Query: 301  LEAPIGNSPSLTCRE----------------GNPRVITL----------SNSYVGLRVKD 360
            L +  G++PS   R                 GN R I +          ++  V  RV D
Sbjct: 745  LNSKKGSNPSYAWRSIHNSLDVIRKGTRWRVGNGRRIHIWDDRWLPTPTTHKVVSPRV-D 804

Query: 361  FLD----------DNNKWKENAIREAFSPQDASDILNMHAGSKDSKDEIVWSFDKKGIFS 420
            + D          D  +WK + I   F P +A+ IL +       +D ++W  +K+G F+
Sbjct: 805  YGDFPMVSSLIDLDTRRWKVDVINATFLPHEANTILKIPLSFNLPEDCLIWIGNKRGEFT 864

Query: 421  VKSAYRLASKGLSTYEASHSNQSKE-AAFWNSIWKANVLPRSKVCAWRIINDIIPTKANA 480
            VKSAY +AS  + + E   S      +  W  IW+  V P+ K+ AWR   + +PT  N 
Sbjct: 865  VKSAYYIASDLVDSVEEGESTSGNSWSLLWKRIWQLKVPPKIKIFAWRTCVNGLPTMQNL 924

Query: 481  LKKGVDLIPLCSFCGKYKESSSHLIWECKMVKSIWSYFVPSSLTM--------------- 540
              +GV     C  C K  E+ +H +  C+  K  W+++  S + +               
Sbjct: 925  NHRGVHCSSFCPLCDKAIETITHALLHCEHAKLTWAFWCNSPVDLSTSCDMVEIALDIIA 984

Query: 541  --------------WSL-------CREDWKPKDYWGW-MEANLNREDIDRSIIIMLRTPA 600
                          WS+         ED        W +   +  E     +   L    
Sbjct: 985  KGSLHDLELFFAVAWSIWWNRNQAIHEDSGSPPIQAWELAGGVLAEFKAACLCPALSQAL 1044

Query: 601  SQAQWEKPRPNTWKLNSDATWMEKEGCRGLGWLVRDSEGSLICFGMKQVKQNWAIKNLEA 651
              ++W+ P    +K+N+DA   + E C  +G ++RD  G ++    K +  ++  +  EA
Sbjct: 1045 PLSRWKAPPSGYFKINTDAAAFDDERCSCIGVVIRDCRGVVLAASSKVLSASFPAEISEA 1104

BLAST of Lag0018774 vs. NCBI nr
Match: XP_030939975.1 (uncharacterized protein LOC115964883 [Quercus lobata])

HSP 1 Score: 391.3 bits (1004), Expect = 1.7e-104
Identity = 246/758 (32.45%), Postives = 357/758 (47.10%), Query Frame = 0

Query: 1    MGFSSNWIQKVMSCISSVCYLVLVNGEHQTEFKPSRGLRQGDPLSPYLFLLCAEGFSALL 60
            +GF + WI  V SCI SV + VLVNGE    F P+RGLRQGDPLSPYLFLLCAEG  +L+
Sbjct: 575  LGFDNRWITLVSSCIRSVSFSVLVNGEPHGNFTPNRGLRQGDPLSPYLFLLCAEGLHSLI 634

Query: 61   ERKESLSKISGFKINKLCPSLTHLFFADDSLVFLKAKYSDLRAFKQVLKHYEGASGQTIN 120
            ++ E    I G  +    P ++HLFFADDSL+F +A   +  +  ++LK YE ASGQ IN
Sbjct: 635  QQAEISGTIKGVSLCSTGPKVSHLFFADDSLLFCRANSQEASSIMEILKQYEEASGQQIN 694

Query: 121  LDKSTFMASKNVKDETLAKCEEVLGIKRSSSLGQYLGMPSQTGRNKGVVFKRIKDRVEKT 180
             +K+    S N       + + +LG+  +++  +YLG+PS  GR K   F  I++R+   
Sbjct: 695  REKTQLFFSPNTDPHVQEEIKTLLGVATTTNYEKYLGLPSFVGRGKKQSFGYIRERIWHK 754

Query: 181  LQGWKGNLFSMGGKEVLIKAVAQAIPVYTMSCFRLPTNICSFIDRLCAKLWWGSAGNKDK 240
            +QGWK  L S GG+EVLIKAV QA+P +TM CF++P ++C  I+ L  K WWG  G   K
Sbjct: 755  MQGWKERLLSQGGREVLIKAVLQAMPTFTMGCFKIPKSLCKDIESLIRKFWWGYKGEARK 814

Query: 241  THWISWKKMCKNKS-----------------PKQSWRLIRNPNSLLFKILRGRYFKGSNF 300
             HW+ WKK+CK+KS                  KQ WRLI N +SL +K+ + ++F   + 
Sbjct: 815  IHWVGWKKLCKSKSHGGLGFKDIELFNIAMLGKQVWRLIHNKDSLFYKVFKAKFFPNCSI 874

Query: 301  LEA--------------------------PIGNSPSLTCREG-------NPRVITLSNSY 360
            L+                            IG+  S+  R         + RV++   ++
Sbjct: 875  LDEGVKENGSYAWQSILKARGVVRMGSKWRIGDGHSVRIRGDKWLPDLFSSRVVSPQKNF 934

Query: 361  V-GLRVKDFLDDNNK-WKENAIREAFSPQDASDILNMHAGSKDSKDEIVWSFDKKGIFSV 420
                RV   +D+ N+ W E+ IRE F P +A  IL++       +D ++W+    G ++ 
Sbjct: 935  PNNTRVCALIDEENRCWMEDRIREEFLPHEAEAILSLPLSFNGKEDRLIWAETANGYYTT 994

Query: 421  KSAYRLASKGLSTYEASHSNQSKEAAFWNSIWKANVLPRSKVCAWRIINDIIPTKANALK 480
            KSAYRL  +         SN + +  FW  +W  NV  + +   WR  ND +PTK N LK
Sbjct: 995  KSAYRLLLQAAEAAAPGTSNPADQKPFWQELWSLNVPNKIRHFLWRAANDSLPTKKNLLK 1054

Query: 481  KGVDLIPLCSFCGKYKESSSHLIWECKMVKSIWSYFVPSSLTMWSL--CREDWKPK---- 540
            + +     C  CG   E   H IW C+M+K +W          W L  CRE    K    
Sbjct: 1055 RNIIQDTTCERCGGEIEDGIHAIWGCQMIKQVW----------WELEKCREFLNEKFASF 1114

Query: 541  -------------------DYWGWM-----------EANLNREDIDRSIIIMLR------ 600
                                + GW              +L  E I R  +  LR      
Sbjct: 1115 HDLLQGILAQKIPNFAELFAFIGWSIWHDRNARRLGSPSLPTEKIYRVAVERLREFHSVQ 1174

Query: 601  -TPASQ------AQWEKPRPNTWKLNSDATWMEKEGCRGLGWLVRDSEGSLICFGMKQVK 657
              P  Q        W  P P+ +K+N D          GLG ++RDSEG +I    +++ 
Sbjct: 1175 EDPRPQLPVHHPTHWLPPSPSVYKVNFDGATFLDIAAAGLGVVIRDSEGLVIAALSERIH 1234

BLAST of Lag0018774 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 1.4e-16
Identity = 42/112 (37.50%), Postives = 60/112 (53.57%), Query Frame = 0

Query: 204 AIPVYTMSCFRLPTNICSFIDRLCAKLWWGSAGNKDKTHWISWKKMCKNKS--------- 263
           A+PVY MSCFRL   +C  +     + WW S  NK K  W++W+K+CK+K          
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 264 ---------PKQSWRLIRNPNSLLFKILRGRYFKGSNFLEAPIGNSPSLTCR 298
                     KQS+R+I  P++LL ++LR RYF  S+ +E  +G  PS   R
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWR 113

BLAST of Lag0018774 vs. ExPASy Swiss-Prot
Match: P92555 (Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 GN=AtMg01250 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 2.4e-13
Identity = 36/76 (47.37%), Postives = 48/76 (63.16%), Query Frame = 0

Query: 18 VCY---LVLVNGEHQTEFKPSRGLRQGDPLSPYLFLLCAEGFSALLERKESLSKISGFKI 77
          VC+   L ++NG  Q    PSRGLRQGDPLSPYLF+LC E  S L  R +   ++ G ++
Sbjct: 5  VCHFYLLFIINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRV 64

Query: 78 NKLCPSLTHLFFADDS 91
          +   P + HL FADD+
Sbjct: 65 SNNSPRINHLLFADDT 80

BLAST of Lag0018774 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 65.5 bits (158), Expect = 2.8e-09
Identity = 57/239 (23.85%), Postives = 106/239 (44.35%), Query Frame = 0

Query: 2   GFSSNWIQKVMSCISSVCYLVLVNGEHQTEFKPSRGLRQGDPLSPYLFLLCAEGFSALLE 61
           G    ++  + +  S     + VNGE         G RQG PLSPYLF +  E  +  + 
Sbjct: 628 GIQGPYLNMIKAIYSKPVANIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVLARAIR 687

Query: 62  RKESLSKISGFKINKLCPSLTHLFFADDSLVFLKAKYSDLRAFKQVLKHYEGASGQTINL 121
           +++   +I G +I K    ++ L  ADD +V++    +  R    ++  +    G  IN 
Sbjct: 688 QQK---EIKGIQIGKEEVKISLL--ADDMIVYISDPKNSTRELLNLINSFGEVVGYKINS 747

Query: 122 DKS-TFMASKNVKDETLAKCEEVLGIKRSSSLGQYLGMPSQTGRNKGVV---FKRIKDRV 181
           +KS  F+ +KN + E   +  E       ++  +YLG+ + T   K +    FK +K  +
Sbjct: 748 NKSMAFLYTKNKQAE--KEIRETTPFSIVTNNIKYLGV-TLTKEVKDLYDKNFKSLKKEI 807

Query: 182 EKTLQGWKGNLFSMGGKEVLIKAVAQAIPVYTMSC--FRLPTNICSFIDRLCAKLWWGS 235
           ++ L+ WK    S  G+  ++K       +Y  +    ++PT   + ++    K  W +
Sbjct: 808 KEDLRRWKDLPCSWIGRINIVKMAILPKAIYRFNAIPIKIPTQFFNELEGAICKFVWNN 858

BLAST of Lag0018774 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 1.3e-06
Identity = 63/248 (25.40%), Postives = 104/248 (41.94%), Query Frame = 0

Query: 3   FSSNWIQKVMSCISSVCYLVLVNGEHQTEFKPSRGLRQGDPLSPYLFLLCAEGFSALLER 62
           F   ++  + +  +S   LV +N          RG+RQG PLS  L+ L  E F  LL +
Sbjct: 616 FGPQFVGYLKTMYASAECLVKINWSLTAPLAFGRGVRQGCPLSGQLYSLAIEPFLCLLRK 675

Query: 63  KESLSKISGFKINKLCPSLTHLFFADDSLVFLKAKYSDLRAFKQVLKHYEGASGQTINLD 122
                +++G  + +    +    +ADD ++ +     DL   ++  + Y  AS   IN  
Sbjct: 676 -----RLTGLVLKEPDMRVVLSAYADD-VILVAQDLVDLERAQECQEVYAAASSARINWS 735

Query: 123 KSTFMASKNVKDETLAKCEEVLGIKRSSSLGQYLGM-PSQTGRNKGVVFKRIKDRVEKTL 182
           KS+ +   ++K + L        I   S + +YLG+  S         F  +++ V   L
Sbjct: 736 KSSGLLEGSLKVDFLPPA--FRDISWESKIIKYLGVYLSAEEYPVSQNFIELEECVLTRL 795

Query: 183 QGWKG--NLFSMGGKEVLIKAVAQAIPVYTMSCFRLPTN--ICSFIDRLCAKLWWGSAGN 242
             WKG   + SM G+ ++I  +  +   Y + C   PT   I     RL   LW G    
Sbjct: 796 GKWKGFAKVLSMRGRALVINQLVASQIWYRLICLS-PTQEFIAKIQRRLLDFLWIGK--- 847

Query: 243 KDKTHWIS 246
               HW+S
Sbjct: 856 ----HWVS 847

BLAST of Lag0018774 vs. ExPASy TrEMBL
Match: M5VU98 (Reverse transcriptase domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_ppa022115mg PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 1.3e-113
Identity = 246/706 (34.84%), Postives = 361/706 (51.13%), Query Frame = 0

Query: 1    MGFSSNWIQKVMSCISSVCYLVLVNGEHQTEFKPSRGLRQGDPLSPYLFLLCAEGFSALL 60
            MGF   W++ VM C+++V Y  LVNGE      P+RGLRQGDPLSPYLFLLCAEGF+ LL
Sbjct: 1014 MGFPILWVRMVMDCVTTVSYSFLVNGEPTRILYPTRGLRQGDPLSPYLFLLCAEGFTTLL 1073

Query: 61   ERKESLSKISGFKINKLCPSLTHLFFADDSLVFLKAKYSDLRAFKQVLKHYEGASGQTIN 120
             + E   ++ G  I +  P+++HLFFADDS VF KA  ++    K + + YE ASGQ IN
Sbjct: 1074 SKAERQGQLQGIVICRGAPTVSHLFFADDSFVFAKATDNNCGVLKHIFEVYEHASGQQIN 1133

Query: 121  LDKSTFMASKNVKDETLAKCEEVLGIKRSSSLGQYLGMPSQTGRNKGVVFKRIKDRVEKT 180
              KS    S N+  +T ++   VLG+ R  S   YLG+P   GRNK V F+ +K+RV K 
Sbjct: 1134 CQKSCVAFSANIHMDTQSRLASVLGVPRVDSHATYLGLPMMLGRNKTVCFRYLKERVWKK 1193

Query: 181  LQGWKGNLFSMGGKEVLIKAVAQAIPVYTMSCFRLPTNICSFIDRLCAKLWWGSAGNKDK 240
            LQGW+    S+ GKEVL+K VAQ+IP+Y MSCF LP  +C  I+++ A+ WWG  G   K
Sbjct: 1194 LQGWREQTLSIAGKEVLLKVVAQSIPLYVMSCFLLPQGLCHEIEQMMARFWWGQQGENRK 1253

Query: 241  THWISWKKMCKNKS-----------------PKQSWRLIRNPNSLLFKILRGRYFKGSNF 300
             HW+ W+++CK K+                  KQ WRL+ NP+SL  ++L+ +YF  +NF
Sbjct: 1254 IHWMRWERLCKAKTEGGMGFRCLQAFNMAMLAKQGWRLVHNPHSLASRLLKAKYFPQTNF 1313

Query: 301  LEAPIGNSPSLTCR------------------EGN----------PRVITLS------NS 360
             EA +G+ PS   +                  +G           PR  T +      + 
Sbjct: 1314 WEATLGSRPSCVWKSIWTARKVLEMGSRFQIGDGKSVRIWGDKWVPRPATFAVITSPLDG 1373

Query: 361  YVGLRVKDFL--DDNNKWKENAIREAFSPQDASDILNMHAGSKDSKDEIVWSFDKKGIFS 420
                +V + +  + + +W    +   F P D  DI+ +    +   D IVW++DK G+F+
Sbjct: 1374 MENTKVSELICNEGSPQWDLQKLNNLFLPVDVVDIVRIPLSIRAPPDRIVWNYDKHGLFT 1433

Query: 421  VKSAYRLASKGLSTYE-ASHSNQSKEAAFWNSIWKANVLPRSKVCAWRIINDIIPTKANA 480
            VKSAYR+A +  S  E  S S+ S     W  IW A V  + K+ AWR+ +DI+PTKAN 
Sbjct: 1434 VKSAYRVALRVTSGDEDESSSSNSDTGMLWRHIWNATVPTKLKIFAWRVAHDILPTKANL 1493

Query: 481  LKKGVDLIPLCSFCGKYKESSSHLIWECKMVKSIWSYFVPSSLTMWSLCREDWKPKDYWG 540
            +KKGVD+  +C FCG   ES+ H++  C    + W+    S LT  +       P +  G
Sbjct: 1494 IKKGVDMQDMCMFCGDITESALHVLAMCPFAVATWNI---SLLTRHAHQGVQRSPHEVVG 1553

Query: 541  WMEANLNR----EDIDRSIIIMLRTPASQAQWEKPRPNTWKLNSDATWMEKEGCRGLGWL 600
            + +  ++      D    +   +R P    +W  P     K N D  +    G   +G +
Sbjct: 1554 FAQQYVHEFITANDTPSKVTDRVRDP---VRWAAPPSGRLKFNFDGAFDPTSGRGAVGVV 1613

Query: 601  VRDSEGSLICFGMKQVKQNWAIKNLEACAMLEGIKEVSDTCNRLGIHLEVETDANEVVRV 649
             RD++G  +    K V +  + ++ E  A  EG+           I    E D+  VV  
Sbjct: 1614 ARDADGGFVAAVAKSVGEVLSAEHAEILAAREGVALALSLGTASPI---FEGDSAVVVSA 1673

BLAST of Lag0018774 vs. ExPASy TrEMBL
Match: A0A803QSN3 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 2.8e-105
Identity = 234/745 (31.41%), Postives = 358/745 (48.05%), Query Frame = 0

Query: 1   MGFSSNWIQKVMSCISSVCYLVLVNGEHQTEFKPSRGLRQGDPLSPYLFLLCAEGFSALL 60
           +G+   W+ K M+CI++V + VLVNGE     +P RGLRQGDPLSPY+FLLC+EGFS L+
Sbjct: 37  LGYDKRWVDKFMNCITTVSFSVLVNGEISGLIQPQRGLRQGDPLSPYMFLLCSEGFSCLI 96

Query: 61  ERKESLSKISGFKINKLCPSLTHLFFADDSLVFLKAKYSDLRAFKQVLKHYEGASGQTIN 120
           +  E   KI G K  +    L+HLFFADDS VFL A  ++ R+ K +L+ Y   SGQ IN
Sbjct: 97  QEAERADKIHGIKFGREGLKLSHLFFADDSFVFLDANPTERRSMKAILQEYSSLSGQQIN 156

Query: 121 LDKSTFMASKNVKDETLAKCEEVLGIKRSSSLGQYLGMPSQTGRNKGVVFKRIKDRVEKT 180
           L KS     + +             +K      +YLG+P+  GR K  VF+ I+ ++   
Sbjct: 157 LQKSELCVGRKINQTDGTMLMHASRVKLVECHTKYLGLPATVGRRKKEVFESIRSKIRDK 216

Query: 181 LQGWKGNLFSMGGKEVLIKAVAQAIPVYTMSCFRLPTNICSFIDRLCAKLWWGSAGNKDK 240
           LQGWK +LFS  G+EVL+KAV QAIP Y MSCFRLP  +   I  + A+ WWGS+ +K+K
Sbjct: 217 LQGWKASLFSQAGREVLLKAVIQAIPTYVMSCFRLPKELIKDIHAMMARFWWGSSESKNK 276

Query: 241 THWISWKKMCKNKS-----------------PKQSWRLIRNPNSLLFKILRGRYFKGSNF 300
            HW  W+K+CK K                   KQ W++I NP+SLL ++L+  YF  S F
Sbjct: 277 IHWGRWEKLCKPKDKGGMGFKDLEKFNQSLLAKQGWKIINNPSSLLARVLKACYFTNSTF 336

Query: 301 LEAPIGNSPSLTCRE------------------------------GNPRVITL---SNSY 360
           +EA +G   S   R                                 P   TL   +   
Sbjct: 337 MEAKLGGFCSFMWRSILWGRKIVERGVRWRVQLGREVRINEDKWLPRPSTFTLRTPTKVN 396

Query: 361 VGLRVKDFLDDNNKWKENAIREAFSPQDASDILNMHAGSKDSKDEIVWSFDKKGIFSVKS 420
            G  +    D+  +W ++ I + F P D   +L M     ++ D++VW +   G+++V S
Sbjct: 397 NGTTMDHLKDEKGEWNQDMITQTFHPDDVPIVLGMAPCYNNAIDDLVWDYTPNGMYTVSS 456

Query: 421 AYRLASKGLSTYEASHSNQSKEAAFWNSIWKANVLPRSKVCAWRIINDIIPTKANALKKG 480
            Y++A+   +   A  S++     +W  IWK  V P+ +  AWR+ N  IP       +G
Sbjct: 457 GYKVAT--TNELNAGPSSEEHTKKWWTEIWKLEVPPKVRNFAWRLYNGWIPVNMVLRNRG 516

Query: 481 VDLIPLCSFCGKYKESSSHLIWECKMVKSIWSY--------------------------- 540
           + + P C  CG  +E+  H IW C  +K IW +                           
Sbjct: 517 MKIDPWCCGCGNEEETIEHGIWFCPAIKEIWQHFLLWKTINRWKGETKHMLMAIKQQTTK 576

Query: 541 -----FVPSSLTMWSLCREDWK-------PKDYW-GW----MEANLNREDIDRSIIIMLR 600
                F+  S  +W+  R + K       P + W  W    M+  ++        +  + 
Sbjct: 577 ADFLMFILLSWLIWN--RRNKKRLKLHSLPNNLWCRWAQLEMDVMMHNHSNSTQPLKPMT 636

Query: 601 TPASQAQWEKPRPNTWKLNSDATWMEKEGCRGLGWLVRDSEGSLICFGMKQVKQNWAIKN 651
           TPA    W+ P P T  +N+DA+ + KE   GLG ++R+ EG+++   +KQ +  ++++ 
Sbjct: 637 TPA--GGWDPPHPGTLCINTDASVLCKEWKIGLGAVIRNHEGAVVAAEIKQQRGCYSVEL 696

BLAST of Lag0018774 vs. ExPASy TrEMBL
Match: A0A7N2LIH6 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 1.1e-104
Identity = 245/749 (32.71%), Postives = 365/749 (48.73%), Query Frame = 0

Query: 1    MGFSSNWIQKVMSCISSVCYLVLVNGEHQTEFKPSRGLRQGDPLSPYLFLLCAEGFSALL 60
            MGF   WI  +M C++SV + VL+NGE +  F PSRGLRQGDP+SPYLFLLC EG SA++
Sbjct: 1292 MGFGDRWISLIMMCVTSVSFSVLINGEPKGSFTPSRGLRQGDPISPYLFLLCGEGLSAMI 1351

Query: 61   ERKESLSKISGFKINKLCPSLTHLFFADDSLVFLKAKYSDLRAFKQVLKHYEGASGQTIN 120
            ++KE    I G    +  P ++HLFFADDS++F +A   +     +VL+ YE  SGQ +N
Sbjct: 1352 KKKEREGLIRGVVAARQAPRISHLFFADDSIIFCRATVDECEQVAKVLEVYEEESGQKLN 1411

Query: 121  LDKSTFMASKNVKDETLAKCEEVLGIKRSSSLGQYLGMPSQTGRNKGVVFKRIKDRVEKT 180
             DK++   S+N KDE     + + G +      +YLG+P   GR K   F RIKD+V + 
Sbjct: 1412 RDKTSLFFSRNTKDEMKEFAKGIFGAQIIQHHEKYLGLPPLIGRAKKKAFNRIKDQVGRK 1471

Query: 181  LQGWKGNLFSMGGKEVLIKAVAQAIPVYTMSCFRLPTNICSFIDRLCAKLWWGSAGNKDK 240
            + GWKG L S  G+EVLIKAVAQA P YTM+ F+LP ++C+ ++ +    WWG  G + K
Sbjct: 1472 IAGWKGKLLSNAGREVLIKAVAQATPTYTMNVFKLPDSLCAELNSMMGSFWWGQRGREKK 1531

Query: 241  THWISWKKMCKNK-----------------SPKQSWRLIRNPNSLLFKILRGRYFKGSNF 300
              W+SWK +CK K                   KQ WRL +NPNSL  ++L+ +YF  S+F
Sbjct: 1532 MAWVSWKNLCKPKVDGGMGFKDLKAFNLALLAKQGWRLHQNPNSLAHRVLKAKYFANSSF 1591

Query: 301  LEAPIGNSPSLTCR----------EGNPRVI------------------------TLSNS 360
            +EA +G  PS   R          EG+  V+                        T S S
Sbjct: 1592 MEAQLGKKPSYIWRSIMAAKNIIKEGSRWVVGDGRSIEIWDARWLPSTASGKVMTTRSGS 1651

Query: 361  YVGLRVKDFL-DDNNKWKENAIREAFSPQDASDILNMHAGSKDSKDEIVWSFDKKGIFSV 420
              G RV   +  +  +WK   +++ F P +A +IL++   S +  D +VW+    G F+V
Sbjct: 1652 VQGERVASLISQERGEWKTTLVQQTFIPHEAEEILSIPLSSMNLADSLVWAETPNGCFTV 1711

Query: 421  KSAYRLASKGLSTYEASHSN-----QSKEAAFWNSIWKANVLPRSKVCAWRIINDIIPTK 480
            KSAYR A K +       +N     +S+ +  W +IW      + K   WR    I+PTK
Sbjct: 1712 KSAYRTAFKCILEPREGEANPECSDKSRMSTIWKTIWGLQCPNKIKHFLWRACRGILPTK 1771

Query: 481  ANALKKGVDLIPLCSFCGKYKESSSHLIWECKMVKSIW----------SYFVPSSLTMWS 540
               + + +     C FCG+  E+S H +W C + K  W           + V     +W 
Sbjct: 1772 KCLVHRKIMKDDCCDFCGE-SETSGHCLWNCIVAKEAWKGLGFNIDNPEHVVEFLDVVWL 1831

Query: 541  LCR----EDWKPKDYWGWM----------------------EANLNREDIDRSIIIMLRT 600
            L      +DW+      W                       EA   RE++  ++    + 
Sbjct: 1832 LLESQGDKDWEFFAIVAWSLWNNRNNVRHGGVSKQGKSITEEARRYREEVRTALPAKGQV 1891

Query: 601  P---ASQAQWEKPRPNTWKLNSDATWMEKEGCRGLGWLVRDSEGSLICFGMKQVKQNWAI 651
            P       +W  P  + +K+N DA    ++G  G+G ++R+++G ++  G    K  + +
Sbjct: 1892 PKPMPKHKRWSPPLQDWYKVNVDAAVFREQGTCGIGVVIRNNKGQIM--GAMSKKMLFPL 1951

BLAST of Lag0018774 vs. ExPASy TrEMBL
Match: A0A5E4GGB8 (PREDICTED: reverse mRNAase (Fragment) OS=Prunus dulcis OX=3755 GN=ALMOND_2B018728 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 1.8e-104
Identity = 204/516 (39.53%), Postives = 290/516 (56.20%), Query Frame = 0

Query: 1   MGFSSNWIQKVMSCISSVCYLVLVNGEHQTEFKPSRGLRQGDPLSPYLFLLCAEGFSALL 60
           MGF   W++ VM C+++V Y  LVNGE      P+RGLRQGDPLSPYLFLLCAEGF+ LL
Sbjct: 212 MGFPIPWVRMVMDCVTTVSYSFLVNGEPTRILYPTRGLRQGDPLSPYLFLLCAEGFTTLL 271

Query: 61  ERKESLSKISGFKINKLCPSLTHLFFADDSLVFLKAKYSDLRAFKQVLKHYEGASGQTIN 120
            + E   ++ G  I +  P+++HLFFADDS VF KA  ++    K + + YE ASGQ IN
Sbjct: 272 SKAERQGQLQGIVICRGAPTVSHLFFADDSFVFAKATDNNCGVLKHIFEVYERASGQQIN 331

Query: 121 LDKSTFMASKNVKDETLAKCEEVLGIKRSSSLGQYLGMPSQTGRNKGVVFKRIKDRVEKT 180
             KS    S N+  +T ++   VLG+ R  S   YLG+P   GRNK   F+ +K+RV K 
Sbjct: 332 CQKSCVAFSTNIHMDTQSRLASVLGVPRVDSHATYLGLPMMLGRNKTFCFRYLKERVWKK 391

Query: 181 LQGWKGNLFSMGGKEVLIKAVAQAIPVYTMSCFRLPTNICSFIDRLCAKLWWGSAGNKDK 240
           LQGW+    S+ GKEVL+K VAQ+IP+Y MSCF LP  +C  I+++ A+ WWG  G   K
Sbjct: 392 LQGWREQTLSIAGKEVLLKVVAQSIPLYVMSCFLLPQGLCHEIEQMMARFWWGQQGENRK 451

Query: 241 THWISWKKMCKNKS-----------------PKQSWRLIRNPNSLLFKILRGRYFKGSNF 300
            HW+ W+++CK K+                  KQ WRL+ NP+SL  ++L+ +YF  +NF
Sbjct: 452 IHWMRWERLCKAKTEGGMGFRCLQAFNMAMLAKQGWRLVHNPHSLASRLLKAKYFPQTNF 511

Query: 301 LEAPIGNSPSLTCR------------------EGN----------PRVITLS------NS 360
            EA +G+ PS   +                  +G           PR  T +      + 
Sbjct: 512 WEATLGSRPSCVWKSIWTARKVLEMGSRFQIGDGKSVRIWGDKWVPRPATFAVITSPLDG 571

Query: 361 YVGLRVKDFL--DDNNKWKENAIREAFSPQDASDILNMHAGSKDSKDEIVWSFDKKGIFS 420
               +V + +  + + +W    +   F P D  DI+ +    +   D IVW++DK G+F+
Sbjct: 572 MENTKVSELICNEGSPQWDLQKLNNLFLPVDVVDIVRIPLSIRAPPDRIVWNYDKHGLFT 631

Query: 421 VKSAYRLASKGLSTYE-ASHSNQSKEAAFWNSIWKANVLPRSKVCAWRIINDIIPTKANA 463
           VKSAYR+A +  S  E  S S+ S  +  W  IW ANV  + K+ AWR+ +DI+PTKAN 
Sbjct: 632 VKSAYRVALRVTSGDEDESSSSNSDTSMLWRHIWNANVPTKLKIFAWRVAHDILPTKANL 691

BLAST of Lag0018774 vs. ExPASy TrEMBL
Match: A0A803P4U9 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 1.8e-104
Identity = 238/732 (32.51%), Postives = 365/732 (49.86%), Query Frame = 0

Query: 1    MGFSSNWIQKVMSCISSVCYLVLVNGEHQTEFKPSRGLRQGDPLSPYLFLLCAEGFSALL 60
            +G+   WI KVM+C+ SV + +L+NG  Q  F P RGLRQGDPLSP+LFLLC+EG + LL
Sbjct: 1081 LGYDEIWISKVMNCVRSVSFSILINGSIQGHFIPERGLRQGDPLSPFLFLLCSEGLTCLL 1140

Query: 61   ERKESLSKISGFKINKLCPSLTHLFFADDSLVFLKAKYSDLRAFKQVLKHYEGASGQTIN 120
               E   KI G +   L  +L+HL FADDSLVFL A   +  A K+VL  Y   SGQ IN
Sbjct: 1141 HESERAGKIHGLRFGNLEHNLSHLLFADDSLVFLNANLEESTALKEVLDCYASLSGQNIN 1200

Query: 121  LDKSTFMASKNVKDETLAKCEEVLGIKRSSSLGQYLGMPSQTGRNKGVVFKRIKDRVEKT 180
            LDKS     + + DE+       LG++   +  +YLGMP+  G+NK  +F +I+DRVE  
Sbjct: 1201 LDKSDLCVGRKINDESAESLAAFLGVRLVKTHTKYLGMPTFVGKNKKEIFGKIRDRVEAK 1260

Query: 181  LQGWKGNLFSMGGKEVLIKAVAQAIPVYTMSCFRLPTNICSFIDRLCAKLWWGSAGNKDK 240
            LQGWK  LFS  GKE+LIKAV QA+P Y MSCFR+   I   I+ + A+ WWG    K K
Sbjct: 1261 LQGWKIGLFSQAGKEILIKAVVQALPCYVMSCFRISKGIIHDIESMIARFWWGENTKKHK 1320

Query: 241  THWISWKKMCKNKS-----------------PKQSWRLIRNPNSLLFKILRGRYFKGSNF 300
             HW SW+KMCK K                   KQ W+++ NP+ LL ++L+  YF  +NF
Sbjct: 1321 IHWGSWEKMCKLKENGGMGFRALEDFNQALLAKQGWKIMTNPDCLLARVLKALYFPNNNF 1380

Query: 301  LEAPIGNSPS------LTCRE----------GNPRVITLS-------NSYVGLR------ 360
            +EA +G+  S      L  RE          GN + + ++        +   LR      
Sbjct: 1381 IEAKLGHFSSNIWRGILWGRELLLKGYRWIIGNGQTVRINEDPWIPRGAPFNLRTKVQVP 1440

Query: 361  ----VKDFLDDNNKWKENAIREAFSPQDASDILNMHAGSKDSKDEIVWSFDKKGIFSVKS 420
                V+  ++ N +WK   I   F+ +D   +L +    ++  D I W+    G+++V S
Sbjct: 1441 KDVTVQTLINANGEWKVEEISGWFNKEDIPWVLGI-TPLREKNDTIGWTLTTNGLYTVAS 1500

Query: 421  AYRLASKGLSTYEASHSNQSKEAAFWNSIWKANVLPRSKVCAWRIINDIIPTKANALKKG 480
             Y+L  +  +  E S ++ S+  A+W  +W + + P+ K+  WR+ +  IP K    K+G
Sbjct: 1501 GYKLRFRDPNIAECSDNSTSR--AWWKVVWGSRLTPKMKIFIWRVFHHWIPVKTELTKRG 1560

Query: 481  VDLIPLCSFCGKYKESSSHLIWECKMVKSIWSYF-----VPSSLTM---------WSLCR 540
            + +   C+ C    E   H +W    +  +W +F      PSSL             L  
Sbjct: 1561 MSMNLTCNRCKSQVEDVCHALWNIPKLHKVWKHFGFLHHFPSSLKQAPDFLMLMKGKLSE 1620

Query: 541  EDWKPKDYWGWMEAN---LNREDIDRSIII-----MLRTPASQAQ-------------WE 600
            E +    +  W + N      +DI+  I I     M+    + AQ             W 
Sbjct: 1621 EFFIGITWLIWYQRNKCVFQNKDIEDDIWIPWATEMMEIHLASAQTNSHQNISKDTVKWS 1680

Query: 601  KPRPNTWKLNSDATWMEKEGCRGLGWLVRDSEGSLICFGMKQVKQNWAIKNLEACAMLEG 648
             P P T+ +N+DA+ +E +   GLG ++RD  G+L+    + +    ++   EA A+   
Sbjct: 1681 PPPPGTFMINTDASLIEGQPGCGLGVIIRDHLGALVTATTEFIPGCLSVLLAEAMAIRLA 1740

BLAST of Lag0018774 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 139.4 bits (350), Expect = 1.1e-32
Identity = 128/572 (22.38%), Postives = 225/572 (39.34%), Query Frame = 0

Query: 204 AIPVYTMSCFRLPTNICSFIDRLCAKLWWGSAGNKDKTHWISWKKMCKNKSP-------- 263
           A+P YTM+CF LP  +C  I  + A  WW +       HW +W  +   K+         
Sbjct: 2   ALPTYTMACFLLPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDI 61

Query: 264 ---------KQSWRLIRNPNSLLFKILRGRYFKGSNFLEAPIGNSPSLT----------C 323
                    KQ WR++  P SL+ K+ + RYF  S+ L AP+G+ PS             
Sbjct: 62  EAFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIHASQEIL 121

Query: 324 REGNPRVITLSNSYV-------------------------------GLRVKDFLDDNNK- 383
           R+G   V+      +                                L+V D +D++ + 
Sbjct: 122 RQGARAVVGNGEDIIIWRHKWLDSKPASAALRMQRVPPQEYASVSSILKVSDLIDESGRE 181

Query: 384 WKENAIREAFSPQDASDILNMHAGSKDSKDEIVWSFDKKGIFSVKSAY----RLASKGLS 443
           W+++ I   F   +   I  +  G +   D   W +   G ++VKS Y    ++ +K  S
Sbjct: 182 WRKDVIEMLFPEVERKLIGELRPGGRRILDSYTWDYTSSGDYTVKSGYWVLTQIINKRSS 241

Query: 444 TYEASHSNQSKEAAFWNSIWKANVLPRSKVCAWRIINDIIPTKANALKKGVDLIPLCSFC 503
             E S  + +     +  IWK+   P+ +   W+ +++ +P       + +     C  C
Sbjct: 242 PQEVSEPSLN---PIYQKIWKSQTSPKIQHFLWKCLSNSLPVAGALAYRHLSKESACIRC 301

Query: 504 GKYKESSSHLIWECKMVKSIWS--------------------YFV------------PSS 563
              KE+ +HL+++C   +  W+                    Y+V             S 
Sbjct: 302 PSCKETVNHLLFKCTFARLTWAISSIPIPLGGEWADSIYVNLYWVFNLGNGNPQWEKASQ 361

Query: 564 LTMWSLCREDWKPKDYWGWMEANLNREDI-----DRSIIIMLRTPA------------SQ 623
           L  W L R  WK ++   +     N +++     D      +RT A            S 
Sbjct: 362 LVPWLLWRL-WKNRNELVFRGREFNAQEVLRRAEDDLEEWRIRTEAESCGTKPQVNRSSC 421

Query: 624 AQWEKPRPNTW-KLNSDATWMEKEGCRGLGWLVRDSEGSLICFGMKQVKQNWAIKNLEAC 663
            +W +P P+ W K N+DATW       G+GW++R+ +G +   G + + +  ++   E  
Sbjct: 422 GRW-RPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELE 481

BLAST of Lag0018774 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 89.7 bits (221), Expect = 9.8e-18
Identity = 42/112 (37.50%), Postives = 60/112 (53.57%), Query Frame = 0

Query: 204 AIPVYTMSCFRLPTNICSFIDRLCAKLWWGSAGNKDKTHWISWKKMCKNKS--------- 263
           A+PVY MSCFRL   +C  +     + WW S  NK K  W++W+K+CK+K          
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 264 ---------PKQSWRLIRNPNSLLFKILRGRYFKGSNFLEAPIGNSPSLTCR 298
                     KQS+R+I  P++LL ++LR RYF  S+ +E  +G  PS   R
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWR 113

BLAST of Lag0018774 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 79.0 bits (193), Expect = 1.7e-14
Identity = 36/76 (47.37%), Postives = 48/76 (63.16%), Query Frame = 0

Query: 18 VCY---LVLVNGEHQTEFKPSRGLRQGDPLSPYLFLLCAEGFSALLERKESLSKISGFKI 77
          VC+   L ++NG  Q    PSRGLRQGDPLSPYLF+LC E  S L  R +   ++ G ++
Sbjct: 5  VCHFYLLFIINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRV 64

Query: 78 NKLCPSLTHLFFADDS 91
          +   P + HL FADD+
Sbjct: 65 SNNSPRINHLLFADDT 80

BLAST of Lag0018774 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 76.3 bits (186), Expect = 1.1e-13
Identity = 80/402 (19.90%), Postives = 156/402 (38.81%), Query Frame = 0

Query: 301 PRVITLSNSYVGLRVKDFLDDNNK---WKENAIREAFSPQDASDILNMHAGSKDSKDEIV 360
           PR +    +Y  + + +  +       W ++ I +     D   I  ++       D+I+
Sbjct: 61  PRPLNTEETYKEMTINNLFERKGSYYFWDDSKISQFVDQSDHGFIHRIYLAKSKKPDKII 120

Query: 361 WSFDKKGIFSVKSAYRLASKGLSTYEASHSNQSKEAAFWNSIWKANVLPRSKVCAWRIIN 420
           W+++  G ++V+S Y L +   ST   + +           IW   ++P+ K   WR ++
Sbjct: 121 WNYNTTGEYTVRSGYWLLTHDPSTNIPAINPPHGSIDLKTRIWNLPIMPKLKHFLWRALS 180

Query: 421 DIIPTKANALKKGVDLIPLCSFCGKYKESSSHLIWECKMVKSIWSYFVPSSLTMWSLCRE 480
             + T      +G+ + P C  C +  ES +H ++ C      W     SSL    L   
Sbjct: 181 QALATTERLTTRGMRIDPSCPRCHRENESINHALFTCPFATMAWR-LSDSSLIRNQLMSN 240

Query: 481 DWKP--------------KDY---------WGWMEANLN------REDIDRSIIIM---- 540
           D++                D+         W   +A  N      RE   ++++      
Sbjct: 241 DFEENISNILNFVQDTTMSDFHKLLPVWLIWRIWKARNNVVFNKFRESPSKTVLSAKAET 300

Query: 541 ----------LRTPA-------SQAQWEKPRPNTWKLNSDATW-MEKEGCRGLGWLVRDS 600
                      +TP+       ++ +W  P     K N DA + ++K    G GW++R+ 
Sbjct: 301 HDWLNATQSHKKTPSPTRQIAENKIEWRNPPATYVKCNFDAGFDVQKLEATG-GWIIRNH 360

Query: 601 EGSLICFGMKQVKQNWAIKNLEACAMLEGIKEVSDTCNRLGIHLEVETDANEVVRVLNGE 649
            G+ I +G  ++         E  A+L  +++   T  R    + +E D   ++ ++NG 
Sbjct: 361 YGTPISWGSMKLAHTSNPLEAETKALLAALQQ---TWIRGYTQVFMEGDCQTLINLINGI 420

BLAST of Lag0018774 vs. TAIR 10
Match: AT2G02520.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 52.8 bits (125), Expect = 1.3e-06
Identity = 24/80 (30.00%), Postives = 37/80 (46.25%), Query Frame = 0

Query: 386 HSNQSKEAAFW-NSIWKANVLPRSKVCAWRIINDIIPTKANALKKGVDLIPLCSFCGKYK 445
           H N   E   W  +IW    +P+    AW  +   + TK   +  G    PLC FC  + 
Sbjct: 33  HLNPIGERVDWFKAIWFKGKIPKHAFIAWVNMRHRLHTKDRMISWGFIFPPLCLFCNTHD 92

Query: 446 ESSSHLIWECKMVKSIWSYF 465
           E+  HL ++C+  + +W YF
Sbjct: 93  ETRQHLFFDCEFAREVWIYF 112

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023881891.11.2e-11033.42uncharacterized protein LOC111994244 [Quercus suber][more]
XP_023878301.14.6e-11033.87uncharacterized protein LOC111990748 [Quercus suber][more]
XP_030946812.14.0e-10632.35uncharacterized protein LOC115971195 [Quercus lobata][more]
XP_023901742.12.6e-10532.84uncharacterized protein LOC112013579 [Quercus suber][more]
XP_030939975.11.7e-10432.45uncharacterized protein LOC115964883 [Quercus lobata][more]
Match NameE-valueIdentityDescription
P932951.4e-1637.50Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 ... [more]
P925552.4e-1347.37Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 ... [more]
P113692.8e-0923.85LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE... [more]
P143811.3e-0625.40Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV... [more]
Match NameE-valueIdentityDescription
M5VU981.3e-11334.84Reverse transcriptase domain-containing protein OS=Prunus persica OX=3760 GN=PRU... [more]
A0A803QSN32.8e-10531.41Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A7N2LIH61.1e-10432.71Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1[more]
A0A5E4GGB81.8e-10439.53PREDICTED: reverse mRNAase (Fragment) OS=Prunus dulcis OX=3755 GN=ALMOND_2B01872... [more]
A0A803P4U91.8e-10432.51Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G29090.11.1e-3222.38Ribonuclease H-like superfamily protein [more]
ATMG00310.19.8e-1837.50RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
ATMG01250.11.7e-1447.37RNA-directed DNA polymerase (reverse transcriptase) [more]
AT3G09510.11.1e-1319.90Ribonuclease H-like superfamily protein [more]
AT2G02520.11.3e-0630.00RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 520..652
e-value: 3.6E-9
score: 38.8
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 4..143
e-value: 2.9E-16
score: 59.7
IPR000477Reverse transcriptase domainPROSITEPS50878RT_POLcoord: 1..160
score: 8.691126
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 366..461
e-value: 4.9E-16
score: 59.2
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 525..650
e-value: 5.4E-17
score: 61.8
NoneNo IPR availablePANTHERPTHR33116:SF45SUBFAMILY NOT NAMEDcoord: 1..425
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 1..425
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 524..648
e-value: 9.48576E-14
score: 66.1836
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 11..131
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 520..653

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0018774.1Lag0018774.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity