Lag0018765 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0018765
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPhytohormone-binding protein-like
Locationchr5: 34023159 .. 34023745 (+)
RNA-Seq ExpressionLag0018765
SyntenyLag0018765
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTAAGGAGGTTAAAGCTGAAGCAAGAGTTGGGGTTGGAGTTGAAACTGTTTGGAAAGCTTTGGTTGAAGACTTGAGATTTCTCATCCCAAAGCTCATGCCCAACACTGTTCAAAACATTGAGCTCCTTCATGGAAATGGTGGCCTTGGCTCTGTCTTTCTTTTCCATCTTGTTCATGGTAACTTTCATTTCACTTTACCCTATTCACTATAATGATTCAAATATTTGATTGAAGAACCTAATTGAGAGAGTTTGAAATTAAAAGAAGAATGATTTTGCATGCAGATGAGGAGATGATGAAAACCCAGAAGGAAAGGATTGTGGAACTTGATGAAACAAAGCATGAAATTGGGATACAAGTGATGGAAGGGAATATATTAAAAAGAGGGTTTTCTTCTTTCAAGACAACATTCAAGCTTTCTTCCATGGCTGAAAAGGAAACCCTAGTGGATGTCAAGGTTGTTTATGAGACTGAAAGACCACATGATGAAGATGAAGAAGCTCACATGGAAGAAATGGCAACTAGGCCAGCTCTTTCTTTCTTTCAACTCTTGGAAAAGTTTCTTCTTCATTCATCTTCTTAA

mRNA sequence

ATGGTTAAGGAGGTTAAAGCTGAAGCAAGAGTTGGGGTTGGAGTTGAAACTGTTTGGAAAGCTTTGGTTGAAGACTTGAGATTTCTCATCCCAAAGCTCATGCCCAACACTGTTCAAAACATTGAGCTCCTTCATGGAAATGGTGGCCTTGGCTCTGTCTTTCTTTTCCATCTTGTTCATGATGAGGAGATGATGAAAACCCAGAAGGAAAGGATTGTGGAACTTGATGAAACAAAGCATGAAATTGGGATACAAGTGATGGAAGGGAATATATTAAAAAGAGGGTTTTCTTCTTTCAAGACAACATTCAAGCTTTCTTCCATGGCTGAAAAGGAAACCCTAGTGGATGTCAAGGTTGTTTATGAGACTGAAAGACCACATGATGAAGATGAAGAAGCTCACATGGAAGAAATGGCAACTAGGCCAGCTCTTTCTTTCTTTCAACTCTTGGAAAAGTTTCTTCTTCATTCATCTTCTTAA

Coding sequence (CDS)

ATGGTTAAGGAGGTTAAAGCTGAAGCAAGAGTTGGGGTTGGAGTTGAAACTGTTTGGAAAGCTTTGGTTGAAGACTTGAGATTTCTCATCCCAAAGCTCATGCCCAACACTGTTCAAAACATTGAGCTCCTTCATGGAAATGGTGGCCTTGGCTCTGTCTTTCTTTTCCATCTTGTTCATGATGAGGAGATGATGAAAACCCAGAAGGAAAGGATTGTGGAACTTGATGAAACAAAGCATGAAATTGGGATACAAGTGATGGAAGGGAATATATTAAAAAGAGGGTTTTCTTCTTTCAAGACAACATTCAAGCTTTCTTCCATGGCTGAAAAGGAAACCCTAGTGGATGTCAAGGTTGTTTATGAGACTGAAAGACCACATGATGAAGATGAAGAAGCTCACATGGAAGAAATGGCAACTAGGCCAGCTCTTTCTTTCTTTCAACTCTTGGAAAAGTTTCTTCTTCATTCATCTTCTTAA

Protein sequence

MVKEVKAEARVGVGVETVWKALVEDLRFLIPKLMPNTVQNIELLHGNGGLGSVFLFHLVHDEEMMKTQKERIVELDETKHEIGIQVMEGNILKRGFSSFKTTFKLSSMAEKETLVDVKVVYETERPHDEDEEAHMEEMATRPALSFFQLLEKFLLHSSS
Homology
BLAST of Lag0018765 vs. NCBI nr
Match: XP_004149307.1 (phytohormone-binding protein [Cucumis sativus] >KGN56215.1 hypothetical protein Csa_010608 [Cucumis sativus])

HSP 1 Score: 249.2 bits (635), Expect = 2.4e-62
Identity = 123/158 (77.85%), Postives = 143/158 (90.51%), Query Frame = 0

Query: 1   MVKEVKAEARVGVGVETVWKALVEDLRFLIPKLMPNTVQNIELLHGNGGLGSVFLFHLVH 60
           MVKEV+AEA+VGVG+ET+WKALV+DLRF++PKLMPNTV+ +EL+HG+GGLGSV LFHLVH
Sbjct: 1   MVKEVEAEAKVGVGIETLWKALVKDLRFIVPKLMPNTVEKVELIHGDGGLGSVLLFHLVH 60

Query: 61  DEEMMKTQKERIVELDETKHEIGIQVMEGNILKRGFSSFKTTFKLSSMAEKETLVDVKVV 120
           DEEMMK QKE+IV+LDETKHE GI+VMEGNILKRGF SF TTF+LSS++EKETLVD KVV
Sbjct: 61  DEEMMKRQKEKIVKLDETKHEFGIEVMEGNILKRGFRSFNTTFRLSSISEKETLVDFKVV 120

Query: 121 YETERPHDEDEEAHMEEMATRPALSFFQLLEKFLLHSS 159
           YETE   DE E+ H+E+MAT  ALSFFQLLE FLLHSS
Sbjct: 121 YETELSDDEVEQTHLEKMATSTALSFFQLLENFLLHSS 158

BLAST of Lag0018765 vs. NCBI nr
Match: KAA0046864.1 (phytohormone-binding protein-like [Cucumis melo var. makuwa] >TYK03282.1 phytohormone-binding protein-like [Cucumis melo var. makuwa])

HSP 1 Score: 244.2 bits (622), Expect = 7.6e-61
Identity = 121/159 (76.10%), Postives = 145/159 (91.19%), Query Frame = 0

Query: 1   MVKEVKAEARVGVGVETVWKALVEDLRFLIPKLMPNTVQNIELLHGNGGLGSVFLFHLVH 60
           MVKEV+AEA++ VG+ET+WKALV+DLRF++PKLMPN+V+ +EL+HG+GGLGSV LFHLVH
Sbjct: 1   MVKEVEAEAKLRVGIETLWKALVKDLRFIVPKLMPNSVEKVELIHGDGGLGSVLLFHLVH 60

Query: 61  DEEMMKTQKERIVELDETKHEIGIQVMEGNILKRGFSSFKTTFKLSSMAEKETLVDVKVV 120
           DEEMMK QKE+IV+LDETKHE+GI+VMEGNILKRGFSSF TTFKLSS++EKETLVD KVV
Sbjct: 61  DEEMMKRQKEKIVKLDETKHELGIEVMEGNILKRGFSSFNTTFKLSSISEKETLVDFKVV 120

Query: 121 YETERPHDEDEEAHMEEMATRPALSFFQLLEKFLLHSSS 160
           YETE   DE E++H+E+MAT  +LSFFQLLE FLL SSS
Sbjct: 121 YETELGDDEVEQSHLEKMATSTSLSFFQLLENFLLDSSS 159

BLAST of Lag0018765 vs. NCBI nr
Match: XP_038904253.1 (phytohormone-binding protein CSBP-like [Benincasa hispida])

HSP 1 Score: 243.8 bits (621), Expect = 9.9e-61
Identity = 122/159 (76.73%), Postives = 141/159 (88.68%), Query Frame = 0

Query: 1   MVKEVKAEARVGVGVETVWKALVEDLRFLIPKLMPNTVQNIELLHGNGGLGSVFLFHLVH 60
           MVKEVKA+A+VGVG+ET+W ALV+DLRF+IPKLMPNTV+ ++L+HGNGGLGSV LFHLVH
Sbjct: 1   MVKEVKAQAKVGVGIETLWNALVKDLRFIIPKLMPNTVEKVDLIHGNGGLGSVLLFHLVH 60

Query: 61  DEEMMKTQKERIVELDETKHEIGIQVMEGNILKRGFSSFKTTFKLSSMAEKETLVDVKVV 120
           DE MM++QKERIVE+DETKHE GI+V+EGNI KRGF SFKT FKLSSM+EKETLVD KVV
Sbjct: 61  DEMMMRSQKERIVEVDETKHEFGIEVIEGNIWKRGFCSFKTIFKLSSMSEKETLVDFKVV 120

Query: 121 YETERPHDEDEEAHMEEMATRPALSFFQLLEKFLLHSSS 160
           YETE   D DE+ HMEE+AT   LSFFQLLEKFLL SS+
Sbjct: 121 YETELRCDGDEQTHMEEIATSTTLSFFQLLEKFLLDSSN 159

BLAST of Lag0018765 vs. NCBI nr
Match: XP_022929437.1 (phytohormone-binding protein-like [Cucurbita moschata])

HSP 1 Score: 241.9 bits (616), Expect = 3.8e-60
Identity = 124/159 (77.99%), Postives = 142/159 (89.31%), Query Frame = 0

Query: 1   MVKEVKAEARVGVGVETVWKALVEDLRFLIPKLMPNTVQNIELLHGNGGLGSVFLFHLVH 60
           MVKE  A+A++GVGVET+WKALVEDLRF+IPKLMPNTV+NIELLHGNGG+GSV LFHL  
Sbjct: 1   MVKEATAQAKLGVGVETLWKALVEDLRFIIPKLMPNTVENIELLHGNGGIGSVLLFHLGP 60

Query: 61  DEEMMKTQKERIVELDETKHEIGIQVMEGNILKRGFSSFKTTFKLSSMAEKETLVDVKVV 120
           D ++MKTQKERIVELDETKHE G++V+EG +LKRGFSSFKTTF LSSM EKETLVD+KVV
Sbjct: 61  DVKIMKTQKERIVELDETKHEFGLEVVEGIMLKRGFSSFKTTFNLSSMGEKETLVDIKVV 120

Query: 121 YETERPHDEDEEAHMEEMATRPALSFFQLLEKFLLHSSS 160
           YETER  D ++E  M+E AT+PALSF QLLEKFLLHSSS
Sbjct: 121 YETER--DGEDEVEMKEAATKPALSFLQLLEKFLLHSSS 157

BLAST of Lag0018765 vs. NCBI nr
Match: XP_008452280.1 (PREDICTED: phytohormone-binding protein-like [Cucumis melo])

HSP 1 Score: 238.4 bits (607), Expect = 4.2e-59
Identity = 122/159 (76.73%), Postives = 142/159 (89.31%), Query Frame = 0

Query: 1   MVKEVKAEARVGVGVETVWKALVEDLRFLIPKLMPNTVQNIELLHGNGGLGSVFLFHLVH 60
           MVKE KA+A+V VG+ET+WKALV+DLRF+IPKLMPNTV+ IEL+HGNGGLGSVFLFHL  
Sbjct: 1   MVKEAKAQAKVRVGIETLWKALVKDLRFIIPKLMPNTVEKIELIHGNGGLGSVFLFHLGR 60

Query: 61  DEEMMKTQKERIVELDETKHEIGIQVMEGNILKRGFSSFKTTFKLSSMAEKETLVDVKVV 120
             +++K QKERIVELDETKHE G++VMEG +LKRGFSSFKTTFKLSSM EKETLVDVKVV
Sbjct: 61  GVKIVKIQKERIVELDETKHEFGLEVMEGILLKRGFSSFKTTFKLSSMKEKETLVDVKVV 120

Query: 121 YETERPHDEDEEAHMEEMATRPALSFFQLLEKFLLHSSS 160
           Y TER  D+++E HMEE+AT+PALSF QLLEKFL+ SSS
Sbjct: 121 YGTER--DDEDEVHMEEVATKPALSFLQLLEKFLIDSSS 157

BLAST of Lag0018765 vs. ExPASy Swiss-Prot
Match: A0A1S3THR8 (Phytohormone-binding protein CSBP OS=Vigna radiata var. radiata OX=3916 GN=CSBP PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 3.1e-25
Identity = 61/160 (38.12%), Postives = 102/160 (63.75%), Query Frame = 0

Query: 1   MVKEVKAEARVGVGVETVWKALVEDLRFLIPKLMPNTVQNIELLHGNGGLGSVFLFHLVH 60
           MVKE   +  + V +E +W  L +D   ++PK++P+ V++++L+ G+GG+G++ +F+ + 
Sbjct: 1   MVKEFNTQTELSVRLEALWAVLSKDFITVVPKVLPHIVKDVQLIEGDGGVGTILIFNFL- 60

Query: 61  DEEMMKTQKERIVELDETKHEIGIQVMEGNILKRGFSSFKTTFKLSSMAEKETLVDVKVV 120
            E     Q+E I E DE+ HEIG+QV+EG  L +G S +KTTFKLS + E +TLV+VK+ 
Sbjct: 61  PEVSPSYQREEITEFDESSHEIGLQVIEGGYLSQGLSYYKTTFKLSEIEEDKTLVNVKIS 120

Query: 121 YETERPHDED-EEAHMEEMATRPALSFFQLLEKFLLHSSS 160
           Y+    HD D EE       ++  L + + LE++L + S+
Sbjct: 121 YD----HDSDIEEKVTPTKTSQSTLMYLRRLERYLSNGSA 155

BLAST of Lag0018765 vs. ExPASy Swiss-Prot
Match: G7J032 (Phytohormone-binding protein OS=Medicago truncatula OX=3880 GN=MTR_3g055120 PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 5.9e-24
Identity = 57/158 (36.08%), Postives = 99/158 (62.66%), Query Frame = 0

Query: 1   MVKEVKAEARVGVGVETVWKALVEDLRFLIPKLMPNTVQNIELLHGNGGLGSVFLFHLVH 60
           M+KE   +  + VG+E +W A  +D+  ++PK++PN V++++++ G+GG+G+  +F+ + 
Sbjct: 1   MIKEFNTQTTLNVGLEALWAAQSKDITLVVPKVLPNIVKDVQVIEGDGGVGTKLIFNFLP 60

Query: 61  DEEMMKTQKERIVELDETKHEIGIQVMEGNILKRGFSSFKTTFKLSSMAEKETLVDVKVV 120
               +  Q+E I E DE  H IG+QV+EG  L +G S +KTTF+ S+++E +TLV+VK+ 
Sbjct: 61  GIAPVNYQREVITEYDELSHTIGLQVVEGGYLNQGLSYYKTTFQFSAISENKTLVNVKIS 120

Query: 121 YETERPHDEDEEAHMEEMATRPALSFFQLLEKFLLHSS 159
           Y+ E   +  EE       +   L +   LEKFLL+ +
Sbjct: 121 YDHE--SELIEEKVKPTKTSESTLFYLGQLEKFLLNGA 156

BLAST of Lag0018765 vs. ExPASy Swiss-Prot
Match: P19418 (Pathogenesis-related protein B OS=Petroselinum crispum OX=4043 GN=PCPR1-3 PE=2 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 3.5e-08
Identity = 41/154 (26.62%), Postives = 77/154 (50.00%), Query Frame = 0

Query: 2   VKEVKAEARVGVGVETVWKALVEDLRFLIPKLMPNTVQNIELLHGNGGLGSVFLFHLVHD 61
           V++ + EA   V  E ++K L  D+  L+P+++P  +++ E L G+GG+G+V L HL  D
Sbjct: 3   VQKSEVEATSSVSAEKLFKGLCLDIDTLLPRVLPGAIKSSETLEGDGGVGTVKLVHL-GD 62

Query: 62  EEMMKTQKERIVELDETKHEIGIQVMEGNILKRGFSSFKTTFKLSSMAEKETLVDVKVVY 121
               KT K+++  +D+        +++G+IL     S    F     A+    V   +++
Sbjct: 63  ASPFKTMKQKVDAIDKATFTYSYSIIDGDILLGFIESINNHFTAVPNADGGCTVKSTIIF 122

Query: 122 ETERPHDEDEEAHMEEMATRPALSFFQLLEKFLL 156
            T+      EE    + A    L+ F+ +E +L+
Sbjct: 123 NTKGDAVVPEE--NIKFANDQNLTIFKAVEAYLI 153

BLAST of Lag0018765 vs. ExPASy Swiss-Prot
Match: P19417 (Pathogenesis-related protein A OS=Petroselinum crispum OX=4043 GN=PCPR1-1 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.3e-07
Identity = 40/154 (25.97%), Postives = 76/154 (49.35%), Query Frame = 0

Query: 2   VKEVKAEARVGVGVETVWKALVEDLRFLIPKLMPNTVQNIELLHGNGGLGSVFLFHLVHD 61
           V++ + E    V  E ++K L  D+  L+P+++P  +++ E L G+GG+G+V L HL  D
Sbjct: 3   VQKSEVETTSSVSAEKLFKGLCLDIDTLLPQVLPGAIKSSETLEGDGGVGTVKLVHL-GD 62

Query: 62  EEMMKTQKERIVELDETKHEIGIQVMEGNILKRGFSSFKTTFKLSSMAEKETLVDVKVVY 121
               KT K+++  +D+        +++G+IL     S    F     A+    V   +++
Sbjct: 63  ASPFKTMKQKVDAIDKATFTYSYSIIDGDILLGFIESINNHFTAVPNADGGCTVKSTIIF 122

Query: 122 ETERPHDEDEEAHMEEMATRPALSFFQLLEKFLL 156
            T+      EE    + A    L+ F+ +E +L+
Sbjct: 123 NTKGDAVVPEE--NIKFANDQNLTIFKAVEAYLI 153

BLAST of Lag0018765 vs. ExPASy Swiss-Prot
Match: P38950 (Major pollen allergen Car b 1 isoform 2 OS=Carpinus betulus OX=12990 PE=1 SV=2)

HSP 1 Score: 50.8 bits (120), Expect = 1.6e-05
Identity = 40/152 (26.32%), Postives = 70/152 (46.05%), Query Frame = 0

Query: 6   KAEARVGVGVETVWKALVEDLRFLIPKLMPNTVQNIELLHGNGGLGSVFLFHLVHDEEMM 65
           +AE    +    ++KA + D   LIPK+ P  V ++E + GNGG G++       +   +
Sbjct: 7   EAETTSVIPAARLFKAFILDGNKLIPKVSPQAVSSVENVEGNGGPGTIKKI-TFSEGSPV 66

Query: 66  KTQKERIVELDETKHEIGIQVMEGNILKRGFSSFKTTFKLSSMAEKETLVDVKVVYETER 125
           K  KER+ E+D T  +    V+EG++L           K+ +     ++V +   +  + 
Sbjct: 67  KYVKERVEEIDHTNFKYNYTVIEGDVLGDKLEKVSHELKIVAAPGGGSIVKISSKFHAKG 126

Query: 126 PHDEDEEAHMEEM--ATRPALSFFQLLEKFLL 156
            H    E + EEM  A   A    + +E +LL
Sbjct: 127 YH----EVNAEEMKGAKEMAEKLLRAVESYLL 153

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004149307.12.4e-6277.85phytohormone-binding protein [Cucumis sativus] >KGN56215.1 hypothetical protein ... [more]
KAA0046864.17.6e-6176.10phytohormone-binding protein-like [Cucumis melo var. makuwa] >TYK03282.1 phytoho... [more]
XP_038904253.19.9e-6176.73phytohormone-binding protein CSBP-like [Benincasa hispida][more]
XP_022929437.13.8e-6077.99phytohormone-binding protein-like [Cucurbita moschata][more]
XP_008452280.14.2e-5976.73PREDICTED: phytohormone-binding protein-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A1S3THR83.1e-2538.13Phytohormone-binding protein CSBP OS=Vigna radiata var. radiata OX=3916 GN=CSBP ... [more]
G7J0325.9e-2436.08Phytohormone-binding protein OS=Medicago truncatula OX=3880 GN=MTR_3g055120 PE=1... [more]
P194183.5e-0826.62Pathogenesis-related protein B OS=Petroselinum crispum OX=4043 GN=PCPR1-3 PE=2 S... [more]
P194171.3e-0725.97Pathogenesis-related protein A OS=Petroselinum crispum OX=4043 GN=PCPR1-1 PE=2 S... [more]
P389501.6e-0526.32Major pollen allergen Car b 1 isoform 2 OS=Carpinus betulus OX=12990 PE=1 SV=2[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000916Bet v I/Major latex proteinPFAMPF00407Bet_v_1coord: 2..154
e-value: 7.0E-15
score: 55.2
IPR023393START-like domain superfamilyGENE3D3.30.530.20coord: 1..157
e-value: 2.6E-36
score: 126.9
NoneNo IPR availablePANTHERPTHR31213FAMILY NOT NAMEDcoord: 3..158
NoneNo IPR availablePANTHERPTHR31213:SF64PHYTOHORMONE-BINDING PROTEINcoord: 3..158
NoneNo IPR availableCDDcd07816Bet_v1-likecoord: 3..132
e-value: 1.16351E-27
score: 98.8008
NoneNo IPR availableSUPERFAMILY55961Bet v1-likecoord: 4..156

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0018765.1Lag0018765.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009738 abscisic acid-activated signaling pathway
biological_process GO:0006952 defense response
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0080163 regulation of protein serine/threonine phosphatase activity
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0010427 abscisic acid binding
molecular_function GO:0004864 protein phosphatase inhibitor activity
molecular_function GO:0038023 signaling receptor activity