Lag0018764 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0018764
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionLEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2
Locationchr5: 34021519 .. 34021947 (+)
RNA-Seq ExpressionLag0018764
SyntenyLag0018764
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATGTGTTTTCCCTCTTTCGATTCCCCATTTCTCTGCTCCATTTTCCACTTCTTCATTCTTCTCTCAACTTCTTCCCATGGCTTTCGAACTCCAAACCCTCTTCAAACCCACTCTTGTTTGCTTCTTTACATTCATCTTCTCGGCCTCAGCTTCTGCAACAACTTCGCTGTACCATAAGTTCCAGGCCTGTGCGCCTAGAAACTGCGGACATGGTCCTGATATCAGTTACCCCTTTTGGATAGATTGTGTGCACGAACCTTCTTGCGGACACCCAGATTTCAAAATCGAATGCAATGCTCAAAACTACCCAGTTCTTGAGTTTTCCCATGAGAACTTCATTGTAGAAGACATTATTTACCAAAACTGTTCGTCTTGGTTGGTCAGTACTGCAGCTTACCTCCCTCCTCCTTTCTCTTTCAGTTAA

mRNA sequence

ATGGCATGTGTTTTCCCTCTTTCGATTCCCCATTTCTCTGCTCCATTTTCCACTTCTTCATTCTTCTCTCAACTTCTTCCCATGGCTTTCGAACTCCAAACCCTCTTCAAACCCACTCTTGTTTGCTTCTTTACATTCATCTTCTCGGCCTCAGCTTCTGCAACAACTTCGCTGTACCATAAGTTCCAGGCCTGTGCGCCTAGAAACTGCGGACATGGTCCTGATATCAGTTACCCCTTTTGGATAGATTGTGTGCACGAACCTTCTTGCGGACACCCAGATTTCAAAATCGAATGCAATGCTCAAAACTACCCAGTTCTTGAGTTTTCCCATGAGAACTTCATTGTAGAAGACATTATTTACCAAAACTGTTCGTCTTGGTTGGTCAGTACTGCAGCTTACCTCCCTCCTCCTTTCTCTTTCAGTTAA

Coding sequence (CDS)

ATGGCATGTGTTTTCCCTCTTTCGATTCCCCATTTCTCTGCTCCATTTTCCACTTCTTCATTCTTCTCTCAACTTCTTCCCATGGCTTTCGAACTCCAAACCCTCTTCAAACCCACTCTTGTTTGCTTCTTTACATTCATCTTCTCGGCCTCAGCTTCTGCAACAACTTCGCTGTACCATAAGTTCCAGGCCTGTGCGCCTAGAAACTGCGGACATGGTCCTGATATCAGTTACCCCTTTTGGATAGATTGTGTGCACGAACCTTCTTGCGGACACCCAGATTTCAAAATCGAATGCAATGCTCAAAACTACCCAGTTCTTGAGTTTTCCCATGAGAACTTCATTGTAGAAGACATTATTTACCAAAACTGTTCGTCTTGGTTGGTCAGTACTGCAGCTTACCTCCCTCCTCCTTTCTCTTTCAGTTAA

Protein sequence

MACVFPLSIPHFSAPFSTSSFFSQLLPMAFELQTLFKPTLVCFFTFIFSASASATTSLYHKFQACAPRNCGHGPDISYPFWIDCVHEPSCGHPDFKIECNAQNYPVLEFSHENFIVEDIIYQNCSSWLVSTAAYLPPPFSFS
Homology
BLAST of Lag0018764 vs. NCBI nr
Match: XP_022134777.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2, partial [Momordica charantia])

HSP 1 Score: 161.0 bits (406), Expect = 7.6e-36
Identity = 81/129 (62.79%), Postives = 93/129 (72.09%), Query Frame = 0

Query: 6   PLSIPHFSAPFSTSSFFSQLLPMAFELQTLFKPTLVCFFTFIFSASASATTSLYHKFQAC 65
           P+S+PHF    S     SQLLPM F+LQTLF PTL+C FT  FS  AS   SL  KF+AC
Sbjct: 19  PISLPHFHLLHS-----SQLLPMDFKLQTLFNPTLICLFTVFFSTFASEIASLDKKFKAC 78

Query: 66  APRNCGHGPDISYPFWIDCVHEPSCGHPDFKIECNAQNYPVLEFSHENFIVEDIIYQNCS 125
           APR+CG+GP+ISYPFWI  VHEP CG+PDFKI CN + YPVLE S E FI+EDI  QN S
Sbjct: 79  APRSCGNGPNISYPFWISGVHEPFCGYPDFKIVCNDEKYPVLEISGEGFIIEDIFPQNHS 138

Query: 126 SWLVSTAAY 135
             LVS +AY
Sbjct: 139 FLLVSPSAY 142

BLAST of Lag0018764 vs. NCBI nr
Match: XP_023531998.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 146.7 bits (369), Expect = 1.5e-31
Identity = 80/138 (57.97%), Postives = 93/138 (67.39%), Query Frame = 0

Query: 2   ACVFPLSIP--HFSAPFSTSSFFSQLLPMAFELQTLFKPTLVCFFTFIFSASASATTSLY 61
           ACVF  SIP   ++ P   S   S L PMA +LQ LFKPTL CFFTFIFS SA       
Sbjct: 78  ACVFFPSIPISLYNFPLLRS---SHLPPMASKLQILFKPTLFCFFTFIFSTSAVPD---- 137

Query: 62  HKFQACAPRNCGHGPDISYPFWIDCVHEPSCGHPDFKIECNAQNYPVLEFSHENFIVEDI 121
           HK   C P++CG+GP+ISYPFWID VH+PSCG PDFKI+CN   YPVLE S E+FI+++I
Sbjct: 138 HKLNPCPPKSCGYGPNISYPFWIDGVHQPSCGDPDFKIKCNEYQYPVLEISDEDFIIQNI 197

Query: 122 IYQNCSSWLVSTAAYLPP 138
             QN S  L S AAY  P
Sbjct: 198 FPQNHSFLLASAAAYDQP 208

BLAST of Lag0018764 vs. NCBI nr
Match: XP_008453431.1 (PREDICTED: LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isoform X1 [Cucumis melo])

HSP 1 Score: 144.1 bits (362), Expect = 9.6e-31
Identity = 79/130 (60.77%), Postives = 90/130 (69.23%), Query Frame = 0

Query: 5   FPLSIPHFSAPFSTSSFFSQLLPMAFELQTLFKPTLVCFFTFIFSASASATTSLYHKFQA 64
           FP+S PH  +          LLPMAF+LQ LFKP+LVCFFTFIFS SAS T      F+ 
Sbjct: 92  FPIS-PHLHS-------LHPLLPMAFKLQILFKPSLVCFFTFIFSTSASET------FKD 151

Query: 65  CAPRNCGHGPDISYPFWIDCVHEPSCGHPDFKIECNAQNYPVLEFSHENFIVEDIIYQNC 124
           C PRNCGHGP I YPFWID V + SCG+PDFKI+C ++ YPVLE S + FIVEDI YQ  
Sbjct: 152 CEPRNCGHGPVIKYPFWIDGVQDSSCGYPDFKIKC-SRKYPVLEISDDIFIVEDIFYQTN 206

Query: 125 SSWLVSTAAY 135
           S  LVS AAY
Sbjct: 212 SFLLVSAAAY 206

BLAST of Lag0018764 vs. NCBI nr
Match: XP_023001712.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 [Cucurbita maxima])

HSP 1 Score: 139.4 bits (350), Expect = 2.4e-29
Identity = 68/110 (61.82%), Postives = 79/110 (71.82%), Query Frame = 0

Query: 28  MAFELQTLFKPTLVCFFTFIFSASASATTSLYHKFQACAPRNCGHGPDISYPFWIDCVHE 87
           MA +LQ LFKPTL CF TFIFS SA       HK   C P++CG+GP+ISYPFWID VHE
Sbjct: 1   MASKLQILFKPTLFCFLTFIFSTSAVPD----HKLNPCPPKSCGYGPNISYPFWIDGVHE 60

Query: 88  PSCGHPDFKIECNAQNYPVLEFSHENFIVEDIIYQNCSSWLVSTAAYLPP 138
           PSCG+PDFKI+CN   YPVLE S E+FI+++I  QN S  L S AAY  P
Sbjct: 61  PSCGYPDFKIKCNEYQYPVLEISDEDFIIQNIFPQNHSFLLASDAAYEQP 106

BLAST of Lag0018764 vs. NCBI nr
Match: KAA0058093.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isoform X1 [Cucumis melo var. makuwa] >TYK28446.1 LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 138.7 bits (348), Expect = 4.0e-29
Identity = 71/107 (66.36%), Postives = 80/107 (74.77%), Query Frame = 0

Query: 28  MAFELQTLFKPTLVCFFTFIFSASASATTSLYHKFQACAPRNCGHGPDISYPFWIDCVHE 87
           MAF+LQ LFKP+LVCFFTFIFS SAS T      F+ C PRNCGHGP I YPFWID V +
Sbjct: 1   MAFKLQILFKPSLVCFFTFIFSTSASET------FKDCEPRNCGHGPVIKYPFWIDGVQD 60

Query: 88  PSCGHPDFKIECNAQNYPVLEFSHENFIVEDIIYQNCSSWLVSTAAY 135
            SCG+PDFKI+C ++ YPVLE S + FIVEDI YQ  S  LVS AAY
Sbjct: 61  SSCGYPDFKIKC-SRKYPVLEISDDIFIVEDIFYQTNSFLLVSAAAY 100

BLAST of Lag0018764 vs. ExPASy Swiss-Prot
Match: P0C5E2 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-1.2 PE=2 SV=3)

HSP 1 Score: 76.3 bits (186), Expect = 3.2e-13
Identity = 37/93 (39.78%), Postives = 57/93 (61.29%), Query Frame = 0

Query: 46  FIFSASASATTSLYHKFQACAPRNCGHGPDISYPFWIDCVHEPSCGHPDFKIEC-NAQNY 105
           F F+  A+ T SL  KF+AC P++CG GP ISYPF++    E  CG+P F++ C + +  
Sbjct: 15  FYFTIIATQTLSLDPKFKACEPKSCGKGPQISYPFYLSGKQESFCGYPSFELTCDDEEKL 74

Query: 106 PVLEFSHENFIVEDIIYQNCSSWLVSTAAYLPP 138
           PVL  S E +++++I Y   S  +V++ A   P
Sbjct: 75  PVLGISGEEYVIKNISYLTQSFQVVNSKASHDP 107

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134777.17.6e-3662.79LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2, par... [more]
XP_023531998.11.5e-3157.97LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 [Cuc... [more]
XP_008453431.19.6e-3160.77PREDICTED: LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-li... [more]
XP_023001712.12.4e-2961.82LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 [Cuc... [more]
KAA0058093.14.0e-2966.36LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isof... [more]
Match NameE-valueIdentityDescription
P0C5E23.2e-1339.78LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 OS=A... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025287Wall-associated receptor kinase, galacturonan-binding domainPFAMPF13947GUB_WAK_bindcoord: 65..128
e-value: 7.5E-16
score: 58.3
NoneNo IPR availablePANTHERPTHR33138OS01G0690200 PROTEINcoord: 41..132
NoneNo IPR availablePANTHERPTHR33138:SF27WALL-ASSOCIATED RECEPTOR KINASE GALACTURONAN-BINDING PROTEINcoord: 41..132

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0018764.1Lag0018764.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
cellular_component GO:0016020 membrane
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0030247 polysaccharide binding
molecular_function GO:0004672 protein kinase activity