Lag0018625 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0018625
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr5: 31120697 .. 31121476 (+)
RNA-Seq ExpressionLag0018625
SyntenyLag0018625
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAACTGATGGGTGCTGCAGTGTCTGCGGCAAGGAGGAAGAAACGGTTAATCATGCTTTGCTAGAATGTGATCCGGCCAGAGCCTTTTGGTTTGGATCACCACTTCAACTGGACGCTGGGAGGTTCCGACAGTTGCATTTCGCTGAAAGTTGGGAGAGAGTGGACCAATGGTTGAGGGAGAGGGATGGGACTGGAGAAGCGCAATGCCTCTTTGCGTTTGGGCTATGGAGGGTGTGGAGATGAAGGAATGAAATGGTGTTTGAGGGGAAGATGATGGATGTTGAGCTGAGCTTAAGGGCTGTGGGATTGGGGAGGGATGGGGACGAGCTGGTGAGTCTAGGGGAGGGAGAGCAGCAATCGGGTAGATAGGGGGAGCGTCTGACGCTGGGGGTGGATGCTGCGTGGGATAGAGCCTCGTTGGCAACAAGGTATGGGTGGGCTGTAGAGGGTTTGGACAGTGTGAGGCAGAGGGAGGAGGCCTGTAGATGGAGGGGGAGTTTGAACAACCTGCATGTTGAGGGTCGGGCTATGAGGTGGGGGTTGGAATGTATGAGGAGCATGGGGGTGAAAAGCCTATACGTCAAATCGGATTCCCTGTGCCTGGTTAATATTGTCAATCATAAAGAGAAGTGCCCAGCTGATTTCGTGCCAATATATGAGATGATTTACCAACTATATGAGTACTTTGATCTGTGTGATTTCCTCTACGTGAAGAGGGATTTGAATGGGAATGCCCATCGCTTGGCCAAATTAGGCCTATGTAATGCTATGATCTAA

mRNA sequence

ATGGGAACTGATGGGTGCTGCAGTGTCTGCGGCAAGGAGGAAGAAACGGTTAATCATGCTTTGCTAGAATGTGATCCGGCCAGAGCCTTTTGGTTTGGATCACCACTTCAACTGGACGCTGGGAGGTTCCGACAGTTGCATTTCGCTGAAAGTTGGGAGAGAGTGGACCAATGGTTGAGGGAGAGGGATGGGACTGGAGAAGCGCAATGCCTCTTTGCGTTTGGGCTATGGAGGGGGGAGCGTCTGACGCTGGGGGTGGATGCTGCGTGGGATAGAGCCTCGTTGGCAACAAGGTATGGGTGGGCTGTAGAGGGTTTGGACAGTGTGAGGCAGAGGGAGGAGGCCTGTAGATGGAGGGGGAGTTTGAACAACCTGCATGTTGAGGGTCGGGCTATGAGGTGGGGGTTGGAATGTATGAGGAGCATGGGGGTGAAAAGCCTATACGTCAAATCGGATTCCCTGTGCCTGGTTAATATTGTCAATCATAAAGAGAAGTGCCCAGCTGATTTCGTGCCAATATATGAGATGATTTACCAACTATATGAGTACTTTGATCTGTGTGATTTCCTCTACGTGAAGAGGGATTTGAATGGGAATGCCCATCGCTTGGCCAAATTAGGCCTATGTAATGCTATGATCTAA

Coding sequence (CDS)

ATGGGAACTGATGGGTGCTGCAGTGTCTGCGGCAAGGAGGAAGAAACGGTTAATCATGCTTTGCTAGAATGTGATCCGGCCAGAGCCTTTTGGTTTGGATCACCACTTCAACTGGACGCTGGGAGGTTCCGACAGTTGCATTTCGCTGAAAGTTGGGAGAGAGTGGACCAATGGTTGAGGGAGAGGGATGGGACTGGAGAAGCGCAATGCCTCTTTGCGTTTGGGCTATGGAGGGGGGAGCGTCTGACGCTGGGGGTGGATGCTGCGTGGGATAGAGCCTCGTTGGCAACAAGGTATGGGTGGGCTGTAGAGGGTTTGGACAGTGTGAGGCAGAGGGAGGAGGCCTGTAGATGGAGGGGGAGTTTGAACAACCTGCATGTTGAGGGTCGGGCTATGAGGTGGGGGTTGGAATGTATGAGGAGCATGGGGGTGAAAAGCCTATACGTCAAATCGGATTCCCTGTGCCTGGTTAATATTGTCAATCATAAAGAGAAGTGCCCAGCTGATTTCGTGCCAATATATGAGATGATTTACCAACTATATGAGTACTTTGATCTGTGTGATTTCCTCTACGTGAAGAGGGATTTGAATGGGAATGCCCATCGCTTGGCCAAATTAGGCCTATGTAATGCTATGATCTAA

Protein sequence

MGTDGCCSVCGKEEETVNHALLECDPARAFWFGSPLQLDAGRFRQLHFAESWERVDQWLRERDGTGEAQCLFAFGLWRGERLTLGVDAAWDRASLATRYGWAVEGLDSVRQREEACRWRGSLNNLHVEGRAMRWGLECMRSMGVKSLYVKSDSLCLVNIVNHKEKCPADFVPIYEMIYQLYEYFDLCDFLYVKRDLNGNAHRLAKLGLCNAMI
Homology
BLAST of Lag0018625 vs. NCBI nr
Match: XP_021763973.1 (uncharacterized protein LOC110728637 [Chenopodium quinoa])

HSP 1 Score: 87.8 bits (216), Expect = 1.2e-13
Identity = 64/232 (27.59%), Postives = 99/232 (42.67%), Query Frame = 0

Query: 4   DGCCSVCGKEEETVNHALLECDPARAFWFGSPLQLDAGRFRQLHFAESWERVDQWLRERD 63
           D  CS+CG  EET+NHAL EC+ A+  W     +          F E W  +   L+E D
Sbjct: 15  DDICSICGNSEETINHALFECEAAKTIWNHCEFRYLLSEAPTGSFGERWNWLSSKLKEYD 74

Query: 64  GTGEAQCLFAFGLWRGERLTLGVDAAWDRASLATRYGWAVEGLDSVRQREEACRW----- 123
               A  L+A   WR   + +  ++  +   +A  Y   VE      +R   CR      
Sbjct: 75  VLRVAGMLWA--AWRCHNMRIYGNSTPNPVMMAANYCKMVEDYQYYAERVYHCRAGLGVV 134

Query: 124 ----RGS-----------LNNLHVEGRAMRWGLECMRSMGVKSLYVKSDSLCLVNIVNHK 183
                GS           L     E  A+ + +  +   G  S +++SD+L +V+ +  +
Sbjct: 135 VRDNNGSMLLTATKKITQLRPDIAEAMAISYAMSTLLRFGYNSAWIESDALNIVSAITKR 194

Query: 184 EKCPADFVPIYEMIYQLYEYFDLCDFLYVKRDLNGNAHRLAK--LGLCNAMI 214
               A    I E I  LY  F++C F +VKR+ N  AH +A+  +G C  +I
Sbjct: 195 HYGAAPIFHILEEIRALYNAFNVCIFSHVKRNSNTVAHLVARGDIGECQELI 244

BLAST of Lag0018625 vs. NCBI nr
Match: XP_019094536.1 (PREDICTED: uncharacterized protein LOC109129945 [Camelina sativa])

HSP 1 Score: 78.6 bits (192), Expect = 7.4e-11
Identity = 67/220 (30.45%), Postives = 87/220 (39.55%), Query Frame = 0

Query: 1   MGTDGCCSVCGKEEETVNHALLECDPARAFWFGSPL-QLDAGRFRQLHFAE----SW--- 60
           +G D  CS C  +EET+NHAL EC PAR          LD+     L  AE    +W   
Sbjct: 472 IGDDALCSRCAAQEETINHALFECAPARQARNDKVFSNLDSNPLALLRIAEDEVKAWALA 531

Query: 61  ---ERVDQWLRERDGTGEAQCLFAFGLWRGERLTLG----VDAAWDRASLATRYGWAVEG 120
              + V   + +  G   A  L   G     R  L     VD +W         GW    
Sbjct: 532 QTEDMVSDMVSDLSG-NIASTLERTGRLGSPRFPLSFMCFVDGSWKATDSYAGRGWFCTS 591

Query: 121 LDSVRQREEACRWRGSLNNLHVEGRAMRWGLECMRSMGVKSLYVKSDSLCLVNIVNHKEK 180
                    A   R SL+ LH E  A+ W + CM     +S+   +D   LV +V+   +
Sbjct: 592 PSGSAHSMGAANLRRSLSPLHAEVEALIWAMRCMIGADNQSVVFLTDCSDLVKMVSSPSE 651

Query: 181 CPADFVPIYEMIYQLYEYFDLCDFLYVKRDLNGNAHRLAK 206
            PA F P  E IY   E F     + V R  NG A  LA+
Sbjct: 652 WPA-FTPYLEDIYADREEFVSFALVCVPRSQNGKADNLAR 689

BLAST of Lag0018625 vs. NCBI nr
Match: XP_022544206.1 (uncharacterized protein LOC111199041 [Brassica napus])

HSP 1 Score: 74.7 bits (182), Expect = 1.1e-09
Identity = 61/218 (27.98%), Postives = 95/218 (43.58%), Query Frame = 0

Query: 1   MGTDGCCSVCGKEEETVNHALLECDPA------RAFWFGSPLQLDAGRFRQLHFAESWER 60
           MGTD  C  C    E++NH L EC PA      + F       +D  +   L  AE W +
Sbjct: 580 MGTDRSCPRCAGPVESINHLLFECPPALQARNDKLFNGKEVSPIDILQHASLE-AEVWRK 639

Query: 61  VDQWLRERDGTGEAQ------CLFAFGLWRGERLTLGVDAAWDRASLATRYGWAV-EGLD 120
            +    E++ T E Q         A  L   +  T  +DA+W      +  GW++ + +D
Sbjct: 640 AN----EKEETNEEQEDSPPTETEAVPLGMAQIPTCQIDASWINNGSVSGLGWSLKDQMD 699

Query: 121 SVRQREEACRWRGSLNNLHVEGRAMRWGLECMRSMGVKSLYVKSDSLCLVNIVNHKEKCP 180
                  AC  + SL+ LH E   + W   CMR M + S+  ++D   LV ++ +  + P
Sbjct: 700 IELFGLRAC--QRSLSALHAEMDGLLWAAACMRDMRITSVRFETDCSDLVEMITNPAEWP 759

Query: 181 ADFVPIYEMIYQLYEYFDLCDFLYVKRDLNGNAHRLAK 206
           A F    E+  ++ E  +     ++ R  NG A  LAK
Sbjct: 760 A-FATEIEVFQRIQEGLENVSLSHISRSRNGRADALAK 789

BLAST of Lag0018625 vs. NCBI nr
Match: XP_010495306.1 (PREDICTED: uncharacterized protein LOC104772378 [Camelina sativa])

HSP 1 Score: 73.9 bits (180), Expect = 1.8e-09
Identity = 61/218 (27.98%), Postives = 90/218 (41.28%), Query Frame = 0

Query: 7   CSVCGKEEETVNHALLECDPARAFWFGSPLQLDAGRFRQLHF-AES-WERVDQWLRERDG 66
           C+ CG E ET+NHA+  C PAR  W  + + + +     LHF +ES +  VD +L   + 
Sbjct: 654 CTRCGAEVETINHAIFRCPPARQVWALAHVPVGS-----LHFPSESVYANVDHFLGSTNP 713

Query: 67  TGEAQCLFAFGLW-----RGER------------LTLGVDAAWDRASLATRYGWAVEGLD 126
             + Q +F + +W     R  R            + +  D +W         GW      
Sbjct: 714 GSQIQ-IFPWLMWYIWKARNARVFENQMEQPEDIVRVAQDGSWKETDAFAGAGWVCTSSQ 773

Query: 127 SVRQREEACRWRGSLNNLHVEGRAMRWGLECMRSMGVKSLYVKSDSLCLVNIVNHKEKCP 186
            V     A  +R SL+ LH E  A  W + CM     + +   +DS  LV +V+     P
Sbjct: 774 GVSPILGATNYRRSLSPLHAEIEAFIWAMRCMIGHDFRDVAFFTDSSDLVKMVSSPHDWP 833

Query: 187 ADFVPIYEMIYQLYEYFDLCDFLYVKRDLNGNAHRLAK 206
           A F    E I    E F      Y+ R+ N  A  LA+
Sbjct: 834 A-FATYLEGIKLDREEFSSFSLSYISRNANVRADSLAR 864

BLAST of Lag0018625 vs. NCBI nr
Match: CAB4321714.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 73.2 bits (178), Expect = 3.1e-09
Identity = 33/74 (44.59%), Postives = 40/74 (54.05%), Query Frame = 0

Query: 5   GCCSVCGKEEETVNHALLECDPARAFWFGSPLQLDAGRFRQLHFAESWERVDQWLRERDG 64
           G C  CG ++ET  H   ECD ARAFWF SPLQLD  +     F  +W+ +   L   + 
Sbjct: 224 GECGFCGFQDETQAHIFFECDFARAFWFASPLQLDVSKLAGEDFISTWQHMMTTLNSSEH 283

Query: 65  TGEAQCLFAFGLWR 79
             EA   F FGLWR
Sbjct: 284 AAEAIQWFVFGLWR 297

BLAST of Lag0018625 vs. ExPASy TrEMBL
Match: A0A6J5YDN0 (Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS50956 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.5e-09
Identity = 33/74 (44.59%), Postives = 40/74 (54.05%), Query Frame = 0

Query: 5   GCCSVCGKEEETVNHALLECDPARAFWFGSPLQLDAGRFRQLHFAESWERVDQWLRERDG 64
           G C  CG ++ET  H   ECD ARAFWF SPLQLD  +     F  +W+ +   L   + 
Sbjct: 224 GECGFCGFQDETQAHIFFECDFARAFWFASPLQLDVSKLAGEDFISTWQHMMTTLNSSEH 283

Query: 65  TGEAQCLFAFGLWR 79
             EA   F FGLWR
Sbjct: 284 AAEAIQWFVFGLWR 297

BLAST of Lag0018625 vs. ExPASy TrEMBL
Match: A0A6J5WPU6 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS20372 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.5e-09
Identity = 33/74 (44.59%), Postives = 40/74 (54.05%), Query Frame = 0

Query: 5   GCCSVCGKEEETVNHALLECDPARAFWFGSPLQLDAGRFRQLHFAESWERVDQWLRERDG 64
           G C  CG ++ET  H   ECD ARAFWF SPLQLD  +     F  +W+ +   L   + 
Sbjct: 669 GECGFCGFQDETQAHIFFECDFARAFWFASPLQLDVSKLAGEDFISTWQHMMTTLNSSEH 728

Query: 65  TGEAQCLFAFGLWR 79
             EA   F FGLWR
Sbjct: 729 AAEAIQWFVFGLWR 742

BLAST of Lag0018625 vs. ExPASy TrEMBL
Match: A0A6J5UE59 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS23353 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.5e-09
Identity = 33/74 (44.59%), Postives = 40/74 (54.05%), Query Frame = 0

Query: 5    GCCSVCGKEEETVNHALLECDPARAFWFGSPLQLDAGRFRQLHFAESWERVDQWLRERDG 64
            G C  CG ++ET  H   ECD ARAFWF SPLQLD  +     F  +W+ +   L   + 
Sbjct: 997  GECGFCGFQDETQAHIFFECDFARAFWFASPLQLDVSKLAGEDFISTWQHMMTTLNSSEH 1056

Query: 65   TGEAQCLFAFGLWR 79
              EA   F FGLWR
Sbjct: 1057 AAEAIQWFVFGLWR 1070

BLAST of Lag0018625 vs. ExPASy TrEMBL
Match: A0A6J5VSP2 (Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS51717 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.5e-09
Identity = 33/74 (44.59%), Postives = 40/74 (54.05%), Query Frame = 0

Query: 5   GCCSVCGKEEETVNHALLECDPARAFWFGSPLQLDAGRFRQLHFAESWERVDQWLRERDG 64
           G C  CG ++ET  H   ECD ARAFWF SPLQLD  +     F  +W+ +   L   + 
Sbjct: 162 GECGFCGFQDETQAHIFFECDFARAFWFASPLQLDVSKLAGEDFISTWQHMMTTLNSSEH 221

Query: 65  TGEAQCLFAFGLWR 79
             EA   F FGLWR
Sbjct: 222 AAEAIQWFVFGLWR 235

BLAST of Lag0018625 vs. ExPASy TrEMBL
Match: A0A6J5UF50 (Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS23354 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.5e-09
Identity = 33/74 (44.59%), Postives = 40/74 (54.05%), Query Frame = 0

Query: 5   GCCSVCGKEEETVNHALLECDPARAFWFGSPLQLDAGRFRQLHFAESWERVDQWLRERDG 64
           G C  CG ++ET  H   ECD ARAFWF SPLQLD  +     F  +W+ +   L   + 
Sbjct: 129 GECGFCGFQDETQAHIFFECDFARAFWFASPLQLDVSKLAGEDFISTWQHMMTTLNSSEH 188

Query: 65  TGEAQCLFAFGLWR 79
             EA   F FGLWR
Sbjct: 189 AAEAIQWFVFGLWR 202

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_021763973.11.2e-1327.59uncharacterized protein LOC110728637 [Chenopodium quinoa][more]
XP_019094536.17.4e-1130.45PREDICTED: uncharacterized protein LOC109129945 [Camelina sativa][more]
XP_022544206.11.1e-0927.98uncharacterized protein LOC111199041 [Brassica napus][more]
XP_010495306.11.8e-0927.98PREDICTED: uncharacterized protein LOC104772378 [Camelina sativa][more]
CAB4321714.13.1e-0944.59unnamed protein product [Prunus armeniaca][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J5YDN01.5e-0944.59Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS50956 PE=... [more]
A0A6J5WPU61.5e-0944.59Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=... [more]
A0A6J5UE591.5e-0944.59Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=... [more]
A0A6J5VSP21.5e-0944.59Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS51717 PE=4 S... [more]
A0A6J5UF501.5e-0944.59Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS23354 PE=4 S... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 86..206
e-value: 1.7E-13
score: 50.5
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 81..211
e-value: 1.1E-13
score: 53.3
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 84..209

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0018625.1Lag0018625.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity