Lag0018602 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0018602 vs. NCBI nr
Match: XP_038906005.1 (gibberellin 2-beta-dioxygenase 8-like [Benincasa hispida])

HSP 1 Score: 601.3 bits (1549), Expect = 5.4e-168
Identity = 297/349 (85.10%), Postives = 320/349 (91.69%), Query Frame = 0

Query: 8   MDIEPPLQVCYYALMKDEVEGKNGRSVVVEVD--RQVVEECE---ELPVIDLGLLKMGKL 67
           M+IEPPL+V YYALMKDE+EGKNGR    E D   +VVEECE   ELP+IDLGLLKMG L
Sbjct: 3   MEIEPPLEVRYYALMKDEMEGKNGRLKAEEEDDHDEVVEECEDEIELPMIDLGLLKMGNL 62

Query: 68  EREKCKREIVEAARNWGFFQVVNHGVPERALKAMMNEQKKVFNEPFAKKSLSNFLNLAGS 127
           E+EKCK EIVEAARNWGFFQ++NHGVPE+ LKAM+ EQKKVFN+PFA KSLSNFLNLAG+
Sbjct: 63  EKEKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFNQPFANKSLSNFLNLAGT 122

Query: 128 YRWGNPVAISPTQISWSEAFHIAVLEVSSLEEHVTLRSTMETVVKKFGSLAESIAEILGQ 187
           YRWGNPVAISPTQISWSEAFHIAVLEVSSL +H+TLRSTME VVKKFG LAE+I+E+LGQ
Sbjct: 123 YRWGNPVAISPTQISWSEAFHIAVLEVSSLNDHLTLRSTMEGVVKKFGRLAENISEVLGQ 182

Query: 188 SLGIKSSYFKERCERGKSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQGQISGLQLM 247
           SLGIKSSYFKERCE+GKSSFRLNRYPPCPFAS+VYGLIPHTDSDYLTILYQ Q SGLQLM
Sbjct: 183 SLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIPHTDSDYLTILYQPQTSGLQLM 242

Query: 248 KDGKWFAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVVASEAFERFSFAYFYCPSDDVM 307
           K GKWF VKPNPQALLVNIGDL QVVSNDVF+SLKHRVVASE  ERFSFAYFYCPSDDVM
Sbjct: 243 KAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVVASEGVERFSFAYFYCPSDDVM 302

Query: 308 IESCLKPTFYRKFSYKEYRQQIEKDVEKTGNKVGLSRFFLHNMLPHLGQ 352
           IES LKP+ YR+FSYKEYRQQIEKDVEKTGNKVGLSRFFLHN+LPHLGQ
Sbjct: 303 IESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSRFFLHNILPHLGQ 351

BLAST of Lag0018602 vs. NCBI nr
Match: XP_022932133.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita moschata] >KAG6577443.1 Gibberellin 2-beta-dioxygenase 8, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015515.1 Gibberellin 2-beta-dioxygenase 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 599.4 bits (1544), Expect = 2.1e-167
Identity = 295/347 (85.01%), Postives = 316/347 (91.07%), Query Frame = 0

Query: 8   MDIEPPLQVCYYALMKDEVEGKNGRSVVVEVDRQVVEEC---EELPVIDLGLLKMGKLER 67
           M+IEPPL V YYALMK+E E KNGR V  E +R+VVEEC   EELPVIDLGLLKMG  ER
Sbjct: 3   MEIEPPLHVRYYALMKEESESKNGRWVGEEEEREVVEECEEEEELPVIDLGLLKMGNSER 62

Query: 68  EKCKREIVEAARNWGFFQVVNHGVPERALKAMMNEQKKVFNEPFAKKSLSNFLNLAGSYR 127
           EKC  E+VEAARNWGFFQV+NHGVPER LK MM EQKKVFN+PFAKKSLSN LNLAG+YR
Sbjct: 63  EKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQPFAKKSLSNVLNLAGTYR 122

Query: 128 WGNPVAISPTQISWSEAFHIAVLEVSSLEEHVTLRSTMETVVKKFGSLAESIAEILGQSL 187
           WGNPVA+SP+QISWSEAFH+AVLEVS+LEEHVTLR TME VVKKFG LAESIAEILGQSL
Sbjct: 123 WGNPVAMSPSQISWSEAFHMAVLEVSNLEEHVTLRPTMEAVVKKFGRLAESIAEILGQSL 182

Query: 188 GIKSSYFKERCERGKSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQGQISGLQLMKD 247
           GIK+ YFKERCE+G+SSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQ Q+SGLQLMKD
Sbjct: 183 GIKACYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQAQVSGLQLMKD 242

Query: 248 GKWFAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVVASEAFERFSFAYFYCPSDDVMIE 307
           GKWFAVKPNP+ALLVNIGDL QVVSND F+SLKHRVVASEA ERFSFAYFY PSDDVMIE
Sbjct: 243 GKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYSPSDDVMIE 302

Query: 308 SCLKPTFYRKFSYKEYRQQIEKDVEKTGNKVGLSRFFLHNMLPHLGQ 352
           SC KP+ YR+FSY+EYRQQIEKDVEKTGNKVGL RFFLHNMLPHLGQ
Sbjct: 303 SCTKPSIYRQFSYREYRQQIEKDVEKTGNKVGLPRFFLHNMLPHLGQ 349

BLAST of Lag0018602 vs. NCBI nr
Match: XP_022985245.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita maxima])

HSP 1 Score: 595.9 bits (1535), Expect = 2.3e-166
Identity = 294/347 (84.73%), Postives = 316/347 (91.07%), Query Frame = 0

Query: 8   MDIEPPLQVCYYALMKDEVEGKNGRSVVVEVDRQVVEEC---EELPVIDLGLLKMGKLER 67
           M+IEPPLQV YYALMK+E E KNGR V  E +R+VVEEC   EELPVIDLGLLKMG  ER
Sbjct: 3   MEIEPPLQVRYYALMKEESESKNGRWVGEEEEREVVEECEEEEELPVIDLGLLKMGNSER 62

Query: 68  EKCKREIVEAARNWGFFQVVNHGVPERALKAMMNEQKKVFNEPFAKKSLSNFLNLAGSYR 127
           EKCK E+VEAARNWGFFQV+NHGVPER LK MM EQ+KVFN+PFAKKSLSN LN+AG+YR
Sbjct: 63  EKCKSEMVEAARNWGFFQVLNHGVPERVLKGMMYEQEKVFNQPFAKKSLSNVLNMAGTYR 122

Query: 128 WGNPVAISPTQISWSEAFHIAVLEVSSLEEHVTLRSTMETVVKKFGSLAESIAEILGQSL 187
           WGNPVA+SP+QISWSEAFH+AVLEVS+L EHVTLR TME VVKKFG LAESIAEILGQSL
Sbjct: 123 WGNPVAMSPSQISWSEAFHMAVLEVSNLGEHVTLRPTMEAVVKKFGRLAESIAEILGQSL 182

Query: 188 GIKSSYFKERCERGKSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQGQISGLQLMKD 247
           GIKSSYFKERCE+G+SSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQ Q+SGLQLMKD
Sbjct: 183 GIKSSYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQAQVSGLQLMKD 242

Query: 248 GKWFAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVVASEAFERFSFAYFYCPSDDVMIE 307
           GKWF+VKPNP+ALLVNIGDL QVVSND F+SLKHRVVASEA ERFSFAYFY PSDDVMIE
Sbjct: 243 GKWFSVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYSPSDDVMIE 302

Query: 308 SCLKPTFYRKFSYKEYRQQIEKDVEKTGNKVGLSRFFLHNMLPHLGQ 352
           S  KP+ YR+FSYKEYRQQIEKDVEKTGNKVGL RF LHNMLPHLGQ
Sbjct: 303 SYTKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLPRFLLHNMLPHLGQ 349

BLAST of Lag0018602 vs. NCBI nr
Match: XP_022942034.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita moschata])

HSP 1 Score: 584.7 bits (1506), Expect = 5.2e-163
Identity = 287/345 (83.19%), Postives = 312/345 (90.43%), Query Frame = 0

Query: 2   DLKNTIMDIEPPLQVCYYALMKDEVEGKNGRSVVVEVDRQVVEECEELPVIDLGLLKMGK 61
           +LK   MDI+PPLQVCY ALMKDEVEGK+GR     V+ +V E  E+LPVIDLGLLKMG 
Sbjct: 9   ELKQLSMDIDPPLQVCYKALMKDEVEGKSGR-----VEDEVEETYEDLPVIDLGLLKMGN 68

Query: 62  LEREKCKREIVEAARNWGFFQVVNHGVPERALKAMMNEQKKVFNEPFAKKSLSNFLNLAG 121
           LEREKCK+EIVEAARNWGFFQV+NHGVPERALKA++ EQKKVF  PFA KSL NFLNL G
Sbjct: 69  LEREKCKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSLGNFLNLGG 128

Query: 122 SYRWGNPVAISPTQISWSEAFHIAVLEVSSLEEHVTLRSTMETVVKKFGSLAESIAEILG 181
           +YRWGNP+A+SP+QISWSEAFHIAVL+VSSLEEH TLRSTMETV KKFGSLAESIAEILG
Sbjct: 129 TYRWGNPLALSPSQISWSEAFHIAVLDVSSLEEHATLRSTMETVAKKFGSLAESIAEILG 188

Query: 182 QSLGIKSSYFKERCERGKSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQGQISGLQL 241
           QSLGIK SYFKERCE+GKSS RLNRYPPCPFAS+VYGLIPHTDSDYLTILYQ   SGLQL
Sbjct: 189 QSLGIKWSYFKERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDSDYLTILYQAHTSGLQL 248

Query: 242 MKDGKWFAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVVASEAFERFSFAYFYCPSDDV 301
           M+DG WF VKPNPQ LLVNIGDLLQVVSNDVFKS+KHRVVASE  ERFSFAYFYCPS+DV
Sbjct: 249 MRDGTWFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPSEDV 308

Query: 302 MIESCLKPTFYRKFSYKEYRQQIEKDVEKTGNKVGLSRFFLHNML 347
           MIESC+KP+ Y++FSYKEYRQQIEKDVEKTGNKVGLSRFFLH+ML
Sbjct: 309 MIESCMKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFLHHML 348

BLAST of Lag0018602 vs. NCBI nr
Match: KAG6600754.1 (Gibberellin 2-beta-dioxygenase 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 584.3 bits (1505), Expect = 6.8e-163
Identity = 287/350 (82.00%), Postives = 314/350 (89.71%), Query Frame = 0

Query: 2   DLKNTIMDIEPPLQVCYYALMKDEVEGKNGRSVVVEVDRQVVEECEELPVIDLGLLKMGK 61
           +LK   MDI+PPLQVCY ALMK EVEGK+GR     V+ +V E  E+LPVIDLGLLKMG 
Sbjct: 9   ELKQLSMDIDPPLQVCYNALMKGEVEGKSGR-----VEDEVEETYEDLPVIDLGLLKMGN 68

Query: 62  LEREKCKREIVEAARNWGFFQVVNHGVPERALKAMMNEQKKVFNEPFAKKSLSNFLNLAG 121
           LEREKCK+EIVEAARNWGFFQV+NHGVPERALKA++ EQKKVF  PFA KSL NFLNL G
Sbjct: 69  LEREKCKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSLGNFLNLGG 128

Query: 122 SYRWGNPVAISPTQISWSEAFHIAVLEVSSLEEHVTLRSTMETVVKKFGSLAESIAEILG 181
           +YRWGNP+A+SP+QISWSEAFHIAVL+VSSLEEHVTLRSTMETV KKFGSLAESIAEILG
Sbjct: 129 TYRWGNPLALSPSQISWSEAFHIAVLDVSSLEEHVTLRSTMETVAKKFGSLAESIAEILG 188

Query: 182 QSLGIKSSYFKERCERGKSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQGQISGLQL 241
           QSLGIK SYFKERCE+GKSS RLNRYPPCPFAS+VYGLIPHTDSDYLTILYQ   SGLQL
Sbjct: 189 QSLGIKWSYFKERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDSDYLTILYQAHTSGLQL 248

Query: 242 MKDGKWFAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVVASEAFERFSFAYFYCPSDDV 301
           M+DG WF VKPNPQ LLVNIGDLLQVVSNDVFKS+KHRVVASE  ERFSFAYFYCPS+DV
Sbjct: 249 MRDGTWFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPSEDV 308

Query: 302 MIESCLKPTFYRKFSYKEYRQQIEKDVEKTGNKVGLSRFFLHNMLPHLGQ 352
           MIESC+KP+ Y++FSYKEYRQQIEKDVEKTGNKVGLSRFFLH+ML  + +
Sbjct: 309 MIESCMKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFLHHMLDSMSK 353

BLAST of Lag0018602 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 334.0 bits (855), Expect = 2.1e-90
Identity = 169/337 (50.15%), Postives = 223/337 (66.17%), Query Frame = 0

Query: 10  IEPPLQVCYYALMKDEVEGKNG--RSVVVEVDRQVVEECEELPVIDLG-LLKMGKLEREK 69
           ++PP    Y  L+ +++  K+     +        V E  ELPVID+  L+   + EREK
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIPFSFSVTAVVEEVELPVIDVSRLIDGAEEEREK 60

Query: 70  CKREIVEAARNWGFFQVVNHGVPERALKAMMNEQKKVFNEPFAKKSLSNFLNLAGSYRWG 129
           CK  I  A+R WGFFQV+NHG+    L+ M  EQ +VF EPF KKS S   + AGSYRWG
Sbjct: 61  CKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEKFS-AGSYRWG 120

Query: 130 NPVAISPTQISWSEAFHIAVLEVSSLEEHVTLRSTMETVVKKFGSLAESIAEILGQSLGI 189
            P A S  Q+SWSEAFH+ + ++S  ++  TL STME    +  +LA  +AE+L +  G 
Sbjct: 121 TPSATSIRQLSWSEAFHVPMTDISDNKDFTTLSSTMEKFASESEALAYMLAEVLAEKSGQ 180

Query: 190 KSSYFKERCERGKSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQGQISGLQLMKDGK 249
            SS+FKE C R     R+NRYPPCP  S+VYGL+PHTDSD+LTILYQ Q+ GLQL+KD +
Sbjct: 181 NSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLIKDNR 240

Query: 250 WFAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVVASEAFERFSFAYFYCPSDDVMIESC 309
           W AVKPNP+AL++NIGDL Q  SN ++KS++HRV+ +   ERFS AYF CPS D +IE  
Sbjct: 241 WIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVMTNPKVERFSTAYFMCPSYDAVIECS 300

Query: 310 LKPTFYRKFSYKEYRQQIEKDVEKTGNKVGLSRFFLH 344
                YR FS++E+RQQ+++DV+K G KVGL RF  H
Sbjct: 301 SDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRFLNH 336

BLAST of Lag0018602 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.3e-76
Identity = 142/298 (47.65%), Postives = 209/298 (70.13%), Query Frame = 0

Query: 48  ELPVIDLGLLKMG-KLEREKCKREIVEAARNWGFFQVVNHGVPERALKAMMNEQKKVFNE 107
           +LPVIDL  L  G +++R++C +++V AA+ WGFFQ+VNHG+P+   + M+ E+KK+F++
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQ 98

Query: 108 PFAKKSLSNFLNLA-GSYRWGNPVAISPTQISWSEAFHIAVLEVSSL-EEHVTLRSTMET 167
           PF+ K    F +L+  SYRWGNP A SP Q S SEAFHI + EVS + ++   LR+ +ET
Sbjct: 99  PFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRISDDRNNLRTIVET 158

Query: 168 VVKKFGSLAESIAEILGQSLGIKSSYFKERCERGKSSFRLNRYPPCPFASQVYGLIPHTD 227
            V++   +A+ I EILG+ + + S YF+   E   S  RLN+Y P  F S+V+GL+PHTD
Sbjct: 159 YVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTD 218

Query: 228 SDYLTILYQGQISGLQLMKDGKWFAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVVASE 287
           + +LTIL Q QI GL+L  +G+W +VKP  +AL VNIGD+ Q +SN V++S++HRV++  
Sbjct: 219 TSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPA 278

Query: 288 AFERFSFAYFYCPSDDVMIESCLKPTFYRKFSYKEYRQQIEKDVEKTGNKVGLSRFFL 343
             ER S A+F CP  +  I+    P  YR+FS++EY++Q E DV++TG+KVGLSRF +
Sbjct: 279 NIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFLI 336

BLAST of Lag0018602 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.4e-73
Identity = 145/349 (41.55%), Postives = 208/349 (59.60%), Query Frame = 0

Query: 10  IEPPLQVCY--YALMKDEVEGKNGRSVVVEVDRQVVEECEELPVIDL-GLLKMGKLEREK 69
           I+PPL   Y   AL++ + +G      V  V         +LP++DL  L + G  ER+ 
Sbjct: 9   IDPPLADSYRALALLRRDRDGGIAPPAVQMVGSGGAVLERDLPMVDLERLTRGGAGERKA 68

Query: 70  CKREIVEAARNWGFFQVVNHGVPERALKAMMNEQKKVFNEPFAKKSLSNFLNLAGSYRWG 129
           C   +  AA  WGFFQ+ NHGV    ++ M  EQ ++F  PF  K  +  LN  GSYRWG
Sbjct: 69  CAGAMARAASEWGFFQLTNHGVGRELMEEMRREQARLFRLPFETKEKAGLLN--GSYRWG 128

Query: 130 NPVAISPTQISWSEAFHIAVLEVSSLE----EHVTLRSTMETVVKKFGSLAESIAEILGQ 189
           NP A S   +SWSEAFH+ +  +S  +    +  +LR  M+ V +    +A ++A  L +
Sbjct: 129 NPTATSLRHLSWSEAFHVPLASISGADCDFGDLTSLRGVMQEVAEAMSRVANTVAAALAE 188

Query: 190 SL-------GIKSSYFKERCERGKSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQGQ 249
            L          + +F   C+      RLNRYP CPFA+  +GL+PHTDSD+LT+L Q Q
Sbjct: 189 ELTGRGGGGASAAPWFPAGCDETTCFLRLNRYPACPFAADTFGLVPHTDSDFLTVLCQDQ 248

Query: 250 ISGLQLMKDGKWFAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVVASEAFERFSFAYFY 309
           + GL LMKD +W AV+P P AL+VNIGDL Q  SN+ +KS++H+VVA+   +R S AYF 
Sbjct: 249 VGGLHLMKDSRWVAVRPRPDALVVNIGDLFQAWSNNRYKSVEHKVVANAKTDRLSVAYFL 308

Query: 310 CPSDDVMIESCLKPTFYRKFSYKEYRQQIEKDVEKTGNKVGLSRFFLHN 345
           CPS D ++ +C +P+ YR F++ EYR+++++DV  TG K+GL  FF H+
Sbjct: 309 CPSYDSLVGTCGEPSPYRAFTFGEYRKKVQEDVRTTGKKIGLPNFFKHS 355

BLAST of Lag0018602 vs. ExPASy Swiss-Prot
Match: Q8LGZ9 (Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX5 PE=1 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 4.0e-65
Identity = 142/308 (46.10%), Postives = 187/308 (60.71%), Query Frame = 0

Query: 43  VEECEELPVIDLGLLKMGKLEREK-CKREIVEAARNWGFFQVVNHGVPERALKAMMNEQK 102
           V+EC ELPVIDL     G +E E+ C+ ++V AA  WGFFQV NHGVP+  L+ + + Q 
Sbjct: 37  VDEC-ELPVIDLA----GLMEAEQVCRADMVRAASEWGFFQVTNHGVPQALLRELHDAQV 96

Query: 103 KVFNEPFAKKSLSNFLNLA-GSYRWGNPVAISPTQISWSEAFHIAVLEVSSLEEHVTLRS 162
            VF  PF +K     L  +  SYRWG P A    Q+SWSEA+HI  +        +  R+
Sbjct: 97  AVFRRPFQEKVTERLLGFSPESYRWGTPTAKCLEQLSWSEAYHIP-MTTPRPSTSIRARA 156

Query: 163 TMETVVKKFGSLAESIAEILGQSLGIKSSYFKERCERGKSSF-RLNRYPPCPFASQVYGL 222
            +E V +    LA+ +AEIL + L            R ++ F RLNRYPPC  A   +GL
Sbjct: 157 VIEEVSRAMYELAQKLAEILMRGLPGAGEGETMVTTREETCFLRLNRYPPCAMAMGGFGL 216

Query: 223 IPHTDSDYLTILYQGQ--ISGLQLMKDGKWFAVKPNPQALLVNIGDLLQVVSNDVFKSLK 282
            PHTDSD LTI++Q Q  + GLQL+K G+W AVKP+P  L+VN+GDLLQ  SNDV+KS++
Sbjct: 217 CPHTDSDLLTIVHQQQDTVGGLQLLKGGRWVAVKPSPSTLIVNVGDLLQAWSNDVYKSVE 276

Query: 283 HRVVASEAFERFSFAYFYCPSDDVMIESCLK-----PTFYRKFSYKEYRQQIEKDVEKTG 341
           HRV+A+   ERFS A+F CPS   +I             YR F++ EYR+QI +DV  TG
Sbjct: 277 HRVMANATLERFSMAFFLCPSYHTLIIPSSSHVHDDDAHYRSFTFGEYRKQIMEDVRSTG 336

BLAST of Lag0018602 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 2.1e-37
Identity = 99/291 (34.02%), Postives = 149/291 (51.20%), Query Frame = 0

Query: 49  LPVIDL-GLLKMGKLEREKCKREIVEAARNWGFFQVVNHGVPERALKAMMNEQKKVFNEP 108
           +P+IDL GL     L  +     I EA R WGFFQVVNHGV    + A     ++ F+ P
Sbjct: 62  IPIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMP 121

Query: 109 F-AKKSLSNFLNLAGSYRWGNPVAISP-TQISWSEAF--HIAVLEVSSLEEHVTLRSTME 168
             AK++ SN       Y  G+ + +     + WS+ +  H+    +    +  +   T+ 
Sbjct: 122 VNAKETYSNSPRTYEGY--GSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIR 181

Query: 169 TVVKKFG----SLAESIAEILGQSLGIKSSYFKERC--ERGKSSFRLNRYPPCPFASQVY 228
            V+ ++G     L+  I  +L  +LG+K   F+E    E   +  R+N YP CP      
Sbjct: 182 EVIDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELAL 241

Query: 229 GLIPHTDSDYLTILY-QGQISGLQLMKDGKWFAVKPNPQALLVNIGDLLQVVSNDVFKSL 288
           GL PH+D   +TIL    Q+ GLQ+ KD  W  VKP+P A +VNIGD +Q++SN  +KS+
Sbjct: 242 GLSPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSV 301

Query: 289 KHRVVASEAFERFSFAYFYCPSDDVMIE------SCLKPTFYRKFSYKEYR 322
           +HRV+ +   ER S A+FY P  D+ I+      S   P  Y   ++ +YR
Sbjct: 302 EHRVIVNSDKERVSLAFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYR 350

BLAST of Lag0018602 vs. ExPASy TrEMBL
Match: A0A6J1F0S8 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111438457 PE=3 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 9.9e-168
Identity = 295/347 (85.01%), Postives = 316/347 (91.07%), Query Frame = 0

Query: 8   MDIEPPLQVCYYALMKDEVEGKNGRSVVVEVDRQVVEEC---EELPVIDLGLLKMGKLER 67
           M+IEPPL V YYALMK+E E KNGR V  E +R+VVEEC   EELPVIDLGLLKMG  ER
Sbjct: 3   MEIEPPLHVRYYALMKEESESKNGRWVGEEEEREVVEECEEEEELPVIDLGLLKMGNSER 62

Query: 68  EKCKREIVEAARNWGFFQVVNHGVPERALKAMMNEQKKVFNEPFAKKSLSNFLNLAGSYR 127
           EKC  E+VEAARNWGFFQV+NHGVPER LK MM EQKKVFN+PFAKKSLSN LNLAG+YR
Sbjct: 63  EKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQPFAKKSLSNVLNLAGTYR 122

Query: 128 WGNPVAISPTQISWSEAFHIAVLEVSSLEEHVTLRSTMETVVKKFGSLAESIAEILGQSL 187
           WGNPVA+SP+QISWSEAFH+AVLEVS+LEEHVTLR TME VVKKFG LAESIAEILGQSL
Sbjct: 123 WGNPVAMSPSQISWSEAFHMAVLEVSNLEEHVTLRPTMEAVVKKFGRLAESIAEILGQSL 182

Query: 188 GIKSSYFKERCERGKSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQGQISGLQLMKD 247
           GIK+ YFKERCE+G+SSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQ Q+SGLQLMKD
Sbjct: 183 GIKACYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQAQVSGLQLMKD 242

Query: 248 GKWFAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVVASEAFERFSFAYFYCPSDDVMIE 307
           GKWFAVKPNP+ALLVNIGDL QVVSND F+SLKHRVVASEA ERFSFAYFY PSDDVMIE
Sbjct: 243 GKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYSPSDDVMIE 302

Query: 308 SCLKPTFYRKFSYKEYRQQIEKDVEKTGNKVGLSRFFLHNMLPHLGQ 352
           SC KP+ YR+FSY+EYRQQIEKDVEKTGNKVGL RFFLHNMLPHLGQ
Sbjct: 303 SCTKPSIYRQFSYREYRQQIEKDVEKTGNKVGLPRFFLHNMLPHLGQ 349

BLAST of Lag0018602 vs. ExPASy TrEMBL
Match: A0A6J1JAT6 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC111483296 PE=3 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 1.1e-166
Identity = 294/347 (84.73%), Postives = 316/347 (91.07%), Query Frame = 0

Query: 8   MDIEPPLQVCYYALMKDEVEGKNGRSVVVEVDRQVVEEC---EELPVIDLGLLKMGKLER 67
           M+IEPPLQV YYALMK+E E KNGR V  E +R+VVEEC   EELPVIDLGLLKMG  ER
Sbjct: 3   MEIEPPLQVRYYALMKEESESKNGRWVGEEEEREVVEECEEEEELPVIDLGLLKMGNSER 62

Query: 68  EKCKREIVEAARNWGFFQVVNHGVPERALKAMMNEQKKVFNEPFAKKSLSNFLNLAGSYR 127
           EKCK E+VEAARNWGFFQV+NHGVPER LK MM EQ+KVFN+PFAKKSLSN LN+AG+YR
Sbjct: 63  EKCKSEMVEAARNWGFFQVLNHGVPERVLKGMMYEQEKVFNQPFAKKSLSNVLNMAGTYR 122

Query: 128 WGNPVAISPTQISWSEAFHIAVLEVSSLEEHVTLRSTMETVVKKFGSLAESIAEILGQSL 187
           WGNPVA+SP+QISWSEAFH+AVLEVS+L EHVTLR TME VVKKFG LAESIAEILGQSL
Sbjct: 123 WGNPVAMSPSQISWSEAFHMAVLEVSNLGEHVTLRPTMEAVVKKFGRLAESIAEILGQSL 182

Query: 188 GIKSSYFKERCERGKSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQGQISGLQLMKD 247
           GIKSSYFKERCE+G+SSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQ Q+SGLQLMKD
Sbjct: 183 GIKSSYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQAQVSGLQLMKD 242

Query: 248 GKWFAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVVASEAFERFSFAYFYCPSDDVMIE 307
           GKWF+VKPNP+ALLVNIGDL QVVSND F+SLKHRVVASEA ERFSFAYFY PSDDVMIE
Sbjct: 243 GKWFSVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYSPSDDVMIE 302

Query: 308 SCLKPTFYRKFSYKEYRQQIEKDVEKTGNKVGLSRFFLHNMLPHLGQ 352
           S  KP+ YR+FSYKEYRQQIEKDVEKTGNKVGL RF LHNMLPHLGQ
Sbjct: 303 SYTKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLPRFLLHNMLPHLGQ 349

BLAST of Lag0018602 vs. ExPASy TrEMBL
Match: A0A6J1FQ56 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111447223 PE=3 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.5e-163
Identity = 287/345 (83.19%), Postives = 312/345 (90.43%), Query Frame = 0

Query: 2   DLKNTIMDIEPPLQVCYYALMKDEVEGKNGRSVVVEVDRQVVEECEELPVIDLGLLKMGK 61
           +LK   MDI+PPLQVCY ALMKDEVEGK+GR     V+ +V E  E+LPVIDLGLLKMG 
Sbjct: 9   ELKQLSMDIDPPLQVCYKALMKDEVEGKSGR-----VEDEVEETYEDLPVIDLGLLKMGN 68

Query: 62  LEREKCKREIVEAARNWGFFQVVNHGVPERALKAMMNEQKKVFNEPFAKKSLSNFLNLAG 121
           LEREKCK+EIVEAARNWGFFQV+NHGVPERALKA++ EQKKVF  PFA KSL NFLNL G
Sbjct: 69  LEREKCKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSLGNFLNLGG 128

Query: 122 SYRWGNPVAISPTQISWSEAFHIAVLEVSSLEEHVTLRSTMETVVKKFGSLAESIAEILG 181
           +YRWGNP+A+SP+QISWSEAFHIAVL+VSSLEEH TLRSTMETV KKFGSLAESIAEILG
Sbjct: 129 TYRWGNPLALSPSQISWSEAFHIAVLDVSSLEEHATLRSTMETVAKKFGSLAESIAEILG 188

Query: 182 QSLGIKSSYFKERCERGKSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQGQISGLQL 241
           QSLGIK SYFKERCE+GKSS RLNRYPPCPFAS+VYGLIPHTDSDYLTILYQ   SGLQL
Sbjct: 189 QSLGIKWSYFKERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDSDYLTILYQAHTSGLQL 248

Query: 242 MKDGKWFAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVVASEAFERFSFAYFYCPSDDV 301
           M+DG WF VKPNPQ LLVNIGDLLQVVSNDVFKS+KHRVVASE  ERFSFAYFYCPS+DV
Sbjct: 249 MRDGTWFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPSEDV 308

Query: 302 MIESCLKPTFYRKFSYKEYRQQIEKDVEKTGNKVGLSRFFLHNML 347
           MIESC+KP+ Y++FSYKEYRQQIEKDVEKTGNKVGLSRFFLH+ML
Sbjct: 309 MIESCMKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFLHHML 348

BLAST of Lag0018602 vs. ExPASy TrEMBL
Match: A0A6J1JF15 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC111484442 PE=3 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.6e-162
Identity = 284/339 (83.78%), Postives = 310/339 (91.45%), Query Frame = 0

Query: 8   MDIEPPLQVCYYALMKDEVEGKNGRSVVVEVDRQVVEECEELPVIDLGLLKMGKLEREKC 67
           MDI+PPLQVCY ALMKDEVEGKNGR     VD +V E  E+LPVIDLGLLKMG LEREKC
Sbjct: 1   MDIDPPLQVCYNALMKDEVEGKNGR-----VDDEVEETYEDLPVIDLGLLKMGNLEREKC 60

Query: 68  KREIVEAARNWGFFQVVNHGVPERALKAMMNEQKKVFNEPFAKKSLSNFLNLAGSYRWGN 127
           K+EIVEAARNWGFFQV+NHGVPERALKA++ EQKKVF  PFA+KSL NFLNL G+YRWGN
Sbjct: 61  KKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAQKSLGNFLNLGGTYRWGN 120

Query: 128 PVAISPTQISWSEAFHIAVLEVSSLEEHVTLRSTMETVVKKFGSLAESIAEILGQSLGIK 187
           P+A+SP+Q+SWSEAFHI VL+VSSLEEHVTLRSTMETV KKFGS+AESIAEILGQSLGIK
Sbjct: 121 PLALSPSQMSWSEAFHIGVLDVSSLEEHVTLRSTMETVAKKFGSIAESIAEILGQSLGIK 180

Query: 188 SSYFKERCERGKSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQGQISGLQLMKDGKW 247
            SYFKERCE+GKSS RLNRYPPCPFAS+VYGLIPHTDSDYLTILYQ   SGLQLM+DG W
Sbjct: 181 WSYFKERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDSDYLTILYQAHTSGLQLMRDGTW 240

Query: 248 FAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVVASEAFERFSFAYFYCPSDDVMIESCL 307
           F VKPNPQ LLVNIGDLLQVVSNDVFKS+KHRVVASE  ERFSFAYFYCPS+DVMIESC+
Sbjct: 241 FPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPSEDVMIESCM 300

Query: 308 KPTFYRKFSYKEYRQQIEKDVEKTGNKVGLSRFFLHNML 347
           KP+ Y++FSYKEYRQQIEKDVEKTGNKVGLSRFFLH++L
Sbjct: 301 KPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFLHHVL 334

BLAST of Lag0018602 vs. ExPASy TrEMBL
Match: A0A6J1E1P5 (gibberellin 2-beta-dioxygenase 8-like OS=Momordica charantia OX=3673 GN=LOC111025151 PE=3 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 3.7e-154
Identity = 279/343 (81.34%), Postives = 312/343 (90.96%), Query Frame = 0

Query: 8   MDIEPPLQVCYYALM-KDEVEGKNGRSVVVEVDRQ-VVEECEELPVIDLGLLKMGKLERE 67
           M+IEPPLQV Y ALM KD  EG+NGR  ++E +R+ VVEEC+ELP+IDLGLLKMG LERE
Sbjct: 1   MEIEPPLQVFYNALMKKDGEEGENGR--ILEDERELVVEECDELPLIDLGLLKMGNLERE 60

Query: 68  KCKREIVEAARNWGFFQVVNHGVPERALKAMMNEQKKVFNEPFAKKSLSNFLNLAGSYRW 127
           KCKR+IVEAARNWGFFQVVNHGVPERALKA+M EQKKVFN+PF KKSL NF+NL+GSYRW
Sbjct: 61  KCKRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLSGSYRW 120

Query: 128 GNPVAISPTQISWSEAFHIAVLEVSSLE-EHVTLRSTMETVVKKFGSLAESIAEILGQSL 187
           GN  AISPTQISWSEAFHIAVLEV SLE ++++LRST+E VVKKFG LAESIAEILGQSL
Sbjct: 121 GNSQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEGVVKKFGGLAESIAEILGQSL 180

Query: 188 GIKSSYFKERCERGKSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQ-GQISGLQLMK 247
           G+KSSYF+E C+RG+SSFRLNRYPPCPFAS+VYGL+PHTD+D+LTILYQ  QI+ L LMK
Sbjct: 181 GMKSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQINALHLMK 240

Query: 248 DGKWFAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVVASEAFERFSFAYFYCPSDDVMI 307
           DG W AVKPNPQALLVNIGDLLQVVSNDVFKSLKHRV+ASEA ERFSFAYFYCPSDDVMI
Sbjct: 241 DGNWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYCPSDDVMI 300

Query: 308 ESCLKPTFYRKFSYKEYRQQIEKDVEKTGNKVGLSRFFLHNML 347
           ESC KP+ YR+FSYKEYRQQIEKDVEKTG+KVGL RF LHN++
Sbjct: 301 ESCTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLHNIM 341

BLAST of Lag0018602 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 334.0 bits (855), Expect = 1.5e-91
Identity = 169/337 (50.15%), Postives = 223/337 (66.17%), Query Frame = 0

Query: 10  IEPPLQVCYYALMKDEVEGKNG--RSVVVEVDRQVVEECEELPVIDLG-LLKMGKLEREK 69
           ++PP    Y  L+ +++  K+     +        V E  ELPVID+  L+   + EREK
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIPFSFSVTAVVEEVELPVIDVSRLIDGAEEEREK 60

Query: 70  CKREIVEAARNWGFFQVVNHGVPERALKAMMNEQKKVFNEPFAKKSLSNFLNLAGSYRWG 129
           CK  I  A+R WGFFQV+NHG+    L+ M  EQ +VF EPF KKS S   + AGSYRWG
Sbjct: 61  CKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEKFS-AGSYRWG 120

Query: 130 NPVAISPTQISWSEAFHIAVLEVSSLEEHVTLRSTMETVVKKFGSLAESIAEILGQSLGI 189
            P A S  Q+SWSEAFH+ + ++S  ++  TL STME    +  +LA  +AE+L +  G 
Sbjct: 121 TPSATSIRQLSWSEAFHVPMTDISDNKDFTTLSSTMEKFASESEALAYMLAEVLAEKSGQ 180

Query: 190 KSSYFKERCERGKSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQGQISGLQLMKDGK 249
            SS+FKE C R     R+NRYPPCP  S+VYGL+PHTDSD+LTILYQ Q+ GLQL+KD +
Sbjct: 181 NSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLIKDNR 240

Query: 250 WFAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVVASEAFERFSFAYFYCPSDDVMIESC 309
           W AVKPNP+AL++NIGDL Q  SN ++KS++HRV+ +   ERFS AYF CPS D +IE  
Sbjct: 241 WIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVMTNPKVERFSTAYFMCPSYDAVIECS 300

Query: 310 LKPTFYRKFSYKEYRQQIEKDVEKTGNKVGLSRFFLH 344
                YR FS++E+RQQ+++DV+K G KVGL RF  H
Sbjct: 301 SDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRFLNH 336

BLAST of Lag0018602 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 288.1 bits (736), Expect = 9.4e-78
Identity = 142/298 (47.65%), Postives = 209/298 (70.13%), Query Frame = 0

Query: 48  ELPVIDLGLLKMG-KLEREKCKREIVEAARNWGFFQVVNHGVPERALKAMMNEQKKVFNE 107
           +LPVIDL  L  G +++R++C +++V AA+ WGFFQ+VNHG+P+   + M+ E+KK+F++
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQ 98

Query: 108 PFAKKSLSNFLNLA-GSYRWGNPVAISPTQISWSEAFHIAVLEVSSL-EEHVTLRSTMET 167
           PF+ K    F +L+  SYRWGNP A SP Q S SEAFHI + EVS + ++   LR+ +ET
Sbjct: 99  PFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRISDDRNNLRTIVET 158

Query: 168 VVKKFGSLAESIAEILGQSLGIKSSYFKERCERGKSSFRLNRYPPCPFASQVYGLIPHTD 227
            V++   +A+ I EILG+ + + S YF+   E   S  RLN+Y P  F S+V+GL+PHTD
Sbjct: 159 YVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTD 218

Query: 228 SDYLTILYQGQISGLQLMKDGKWFAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVVASE 287
           + +LTIL Q QI GL+L  +G+W +VKP  +AL VNIGD+ Q +SN V++S++HRV++  
Sbjct: 219 TSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPA 278

Query: 288 AFERFSFAYFYCPSDDVMIESCLKPTFYRKFSYKEYRQQIEKDVEKTGNKVGLSRFFL 343
             ER S A+F CP  +  I+    P  YR+FS++EY++Q E DV++TG+KVGLSRF +
Sbjct: 279 NIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFLI 336

BLAST of Lag0018602 vs. TAIR 10
Match: AT5G05600.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 157.9 bits (398), Expect = 1.5e-38
Identity = 99/291 (34.02%), Postives = 149/291 (51.20%), Query Frame = 0

Query: 49  LPVIDL-GLLKMGKLEREKCKREIVEAARNWGFFQVVNHGVPERALKAMMNEQKKVFNEP 108
           +P+IDL GL     L  +     I EA R WGFFQVVNHGV    + A     ++ F+ P
Sbjct: 62  IPIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMP 121

Query: 109 F-AKKSLSNFLNLAGSYRWGNPVAISP-TQISWSEAF--HIAVLEVSSLEEHVTLRSTME 168
             AK++ SN       Y  G+ + +     + WS+ +  H+    +    +  +   T+ 
Sbjct: 122 VNAKETYSNSPRTYEGY--GSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIR 181

Query: 169 TVVKKFG----SLAESIAEILGQSLGIKSSYFKERC--ERGKSSFRLNRYPPCPFASQVY 228
            V+ ++G     L+  I  +L  +LG+K   F+E    E   +  R+N YP CP      
Sbjct: 182 EVIDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELAL 241

Query: 229 GLIPHTDSDYLTILY-QGQISGLQLMKDGKWFAVKPNPQALLVNIGDLLQVVSNDVFKSL 288
           GL PH+D   +TIL    Q+ GLQ+ KD  W  VKP+P A +VNIGD +Q++SN  +KS+
Sbjct: 242 GLSPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSV 301

Query: 289 KHRVVASEAFERFSFAYFYCPSDDVMIE------SCLKPTFYRKFSYKEYR 322
           +HRV+ +   ER S A+FY P  D+ I+      S   P  Y   ++ +YR
Sbjct: 302 EHRVIVNSDKERVSLAFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYR 350

BLAST of Lag0018602 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 157.1 bits (396), Expect = 2.5e-38
Identity = 87/288 (30.21%), Postives = 138/288 (47.92%), Query Frame = 0

Query: 47  EELPVIDLGLLKMGKLEREKCKREIVEAARNWGFFQVVNHGVPERALKAMMNEQKKVFNE 106
           + +P+IDL    +    R     +   A  + GFFQ+ NHGVPE  +K MMN  ++ F +
Sbjct: 40  DSIPLIDLH--DLHGPNRADIINQFAHACSSCGFFQIKNHGVPEETIKKMMNAAREFFRQ 99

Query: 107 PFAKKSLSNFLNLAGSYRWGNPVAISPTQIS-WSEAFHIAVLEVSSL-----EEHVTLRS 166
             +++      +   + R      +S  ++S W +   +    +           ++ R 
Sbjct: 100 SESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCYPIEDFINEWPSTPISFRE 159

Query: 167 TMETVVKKFGSLAESIAEILGQSLGIKSSYFKERCERGKSSFRLNRYPPCPFASQVYGLI 226
                     +L  ++ E + +SLG+          +      +N YP CP     YGL 
Sbjct: 160 VTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHGQHMAINYYPRCPQPELTYGLP 219

Query: 227 PHTDSDYLTILYQGQISGLQLMKDGKWFAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRV 286
            H D++ +T+L Q ++SGLQ+ KDGKW AV P P   +VN+GD +QV+SN+ +KS+ HR 
Sbjct: 220 GHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRA 279

Query: 287 VASEAFERFSFAYFYCPSDDVMIESCLK--------PTFYRKFSYKEY 321
           V +   ER S   FYCPS+D +I    +        P  YR F+Y EY
Sbjct: 280 VVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEY 325

BLAST of Lag0018602 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 152.1 bits (383), Expect = 8.1e-37
Identity = 93/289 (32.18%), Postives = 152/289 (52.60%), Query Frame = 0

Query: 48  ELPVIDL-GLLKMGKLEREKCKREIVEAARNWGFFQVVNHGVPERALKAMMNEQKKVFNE 107
           E+PVID+  L  +  ++ E  K +   A ++WGFFQ+VNHG+    L+ +  E ++ FN 
Sbjct: 52  EIPVIDMTRLCSVSAMDSELKKLDF--ACQDWGFFQLVNHGIDSSFLEKLETEVQEFFNL 111

Query: 108 PFAKKSLSNFLNLAGSYR-WGNPVAISPTQ-ISWSEAFHIAVLEVSSLEEHV------TL 167
           P  +K        +G +  +G    +S  Q + W + F +    + S + H+        
Sbjct: 112 PMKEK--QKLWQRSGEFEGFGQVNIVSENQKLDWGDMFILTTEPIRSRKSHLFSKLPPPF 171

Query: 168 RSTMETVVKKFGSLAESIAEILGQSLGIKSSYFKERCERGKSSFRLNRYPPCPFASQVYG 227
           R T+ET   +  S+A+ +   +   L IK    ++  +    S ++N YPPCP   QV G
Sbjct: 172 RETLETYSSEVKSIAKILFAKMASVLEIKHEEMEDLFDDVWQSIKINYYPPCPQPDQVMG 231

Query: 228 LIPHTDSDYLTILYQ-GQISGLQLMKDGKWFAVKPNPQALLVNIGDLLQVVSNDVFKSLK 287
           L  H+D+  LTIL Q  Q+ GLQ+ KDGKW  VKP   AL+VN+G++L++++N  ++S++
Sbjct: 232 LTQHSDAAGLTILLQVNQVEGLQIKKDGKWVVVKPLRDALVVNVGEILEIITNGRYRSIE 291

Query: 288 HRVVASEAFERFSFAYFYCPSDDVMIESC------LKPTFYRKFSYKEY 321
           HR V +   ER S A F+ P  + +I          K   ++  S +EY
Sbjct: 292 HRAVVNSEKERLSVAMFHSPGKETIIRPAKSLVDRQKQCLFKSMSTQEY 336