Lag0018533 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0018533 vs. NCBI nr
Match: XP_022942616.1 (2S sulfur-rich seed storage protein 2-like [Cucurbita moschata])

HSP 1 Score: 236.9 bits (603), Expect = 1.1e-58
Identity = 117/140 (83.57%), Postives = 127/140 (90.71%), Query Frame = 0

Query: 1   MATHPISILLIALFLFAAAASALHFDDDNQRPWLQRCRQQIEGPRLRHCEQLLTRPRRVR 60
           MA HP S+LL+ L LF  AASAL F DDNQRPWLQRCRQQ+ G RLRHCEQLL RPRRVR
Sbjct: 1   MARHPFSLLLL-LSLFLLAASALRFHDDNQRPWLQRCRQQVRGERLRHCEQLLMRPRRVR 60

Query: 61  QLLVSEEEEDAVEHLEECCRQLRGMDDPCRCEGLRQIVQRRRQQGRLQGIDVGQMMERAT 120
           QLL++EEEEDAVEHLE+CC+QLRGMDD CRCEGLRQIVQRRRQQGR+QGIDVGQM+ERAT
Sbjct: 61  QLLMAEEEEDAVEHLEQCCQQLRGMDDACRCEGLRQIVQRRRQQGRMQGIDVGQMLERAT 120

Query: 121 NLPSVCRLSQRRCDTRSSRW 141
           NLPSVCRLSQRRC+ RSSRW
Sbjct: 121 NLPSVCRLSQRRCELRSSRW 139

BLAST of Lag0018533 vs. NCBI nr
Match: XP_022984798.1 (2S sulfur-rich seed storage protein 2-like [Cucurbita maxima])

HSP 1 Score: 236.1 bits (601), Expect = 1.8e-58
Identity = 117/140 (83.57%), Postives = 126/140 (90.00%), Query Frame = 0

Query: 1   MATHPISILLIALFLFAAAASALHFDDDNQRPWLQRCRQQIEGPRLRHCEQLLTRPRRVR 60
           MA HP S+LL+ L LF  AASAL F DDNQ PWLQRCRQQ+ G RLRHCEQLL RPRRVR
Sbjct: 1   MARHPFSLLLL-LSLFLLAASALRFHDDNQNPWLQRCRQQVRGERLRHCEQLLMRPRRVR 60

Query: 61  QLLVSEEEEDAVEHLEECCRQLRGMDDPCRCEGLRQIVQRRRQQGRLQGIDVGQMMERAT 120
           QLL++EEEEDAVEHLEECC+QLRGMDD CRCEGLRQIVQRRRQQGR+QGIDVGQM+ERAT
Sbjct: 61  QLLMAEEEEDAVEHLEECCQQLRGMDDACRCEGLRQIVQRRRQQGRMQGIDVGQMLERAT 120

Query: 121 NLPSVCRLSQRRCDTRSSRW 141
           NLPSVCRLSQRRC+ RSSRW
Sbjct: 121 NLPSVCRLSQRRCELRSSRW 139

BLAST of Lag0018533 vs. NCBI nr
Match: KAG6600762.1 (hypothetical protein SDJN03_05995, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 235.7 bits (600), Expect = 2.4e-58
Identity = 116/140 (82.86%), Postives = 127/140 (90.71%), Query Frame = 0

Query: 1   MATHPISILLIALFLFAAAASALHFDDDNQRPWLQRCRQQIEGPRLRHCEQLLTRPRRVR 60
           MA HP S+LL+ L LF  AASAL F DDNQRPWLQRCRQQ+ G RLRHCEQLL RPRRVR
Sbjct: 1   MARHPFSLLLL-LSLFLLAASALRFHDDNQRPWLQRCRQQVRGERLRHCEQLLMRPRRVR 60

Query: 61  QLLVSEEEEDAVEHLEECCRQLRGMDDPCRCEGLRQIVQRRRQQGRLQGIDVGQMMERAT 120
           QL+++EEEEDAVEHLE+CC+QLRGMDD CRCEGLRQIVQRRRQQGR+QGIDVGQM+ERAT
Sbjct: 61  QLVMAEEEEDAVEHLEQCCQQLRGMDDACRCEGLRQIVQRRRQQGRMQGIDVGQMLERAT 120

Query: 121 NLPSVCRLSQRRCDTRSSRW 141
           NLPSVCRLSQRRC+ RSSRW
Sbjct: 121 NLPSVCRLSQRRCELRSSRW 139

BLAST of Lag0018533 vs. NCBI nr
Match: XP_008463441.1 (PREDICTED: 2S sulfur-rich seed storage protein 2-like [Cucumis melo])

HSP 1 Score: 216.9 bits (551), Expect = 1.1e-52
Identity = 112/142 (78.87%), Postives = 126/142 (88.73%), Query Frame = 0

Query: 1   MATHP-ISILLIALFLFAAAASALHFDDDN--QRPWLQRCRQQIE-GPRLRHCEQLLTRP 60
           M+T P +SILL++LFL A AAS   FDD+N  QRPWLQRCRQQI  G RLRHCE+LLTRP
Sbjct: 34  MSTKPLVSILLLSLFLLAVAASDHQFDDENQRQRPWLQRCRQQIRGGERLRHCERLLTRP 93

Query: 61  RRVRQLLVSEEEEDAVEHLEECCRQLRGMDDPCRCEGLRQIVQRRRQQGRLQGIDVGQMM 120
           RR RQLL +EEEEDAVEHLEECC+QLRGMDD CRCEGLRQI+QRRRQQG+LQG+DVGQM+
Sbjct: 94  RRFRQLLAAEEEEDAVEHLEECCQQLRGMDDACRCEGLRQIIQRRRQQGQLQGMDVGQML 153

Query: 121 ERATNLPSVCRLSQRRCDTRSS 139
           ERATNLPSVCRLS+RRCD RS+
Sbjct: 154 ERATNLPSVCRLSRRRCDLRST 175

BLAST of Lag0018533 vs. NCBI nr
Match: XP_031738807.1 (2S sulfur-rich seed storage protein 2-like [Cucumis sativus])

HSP 1 Score: 216.5 bits (550), Expect = 1.5e-52
Identity = 112/142 (78.87%), Postives = 126/142 (88.73%), Query Frame = 0

Query: 1   MATHP-ISILLIALFLFAAAASALHFDDDN--QRPWLQRCRQQI-EGPRLRHCEQLLTRP 60
           M+T P +SILL++LFLFA AAS   FDDDN  QRPWLQRCRQQI  G RLRHCE+LLT+P
Sbjct: 1   MSTKPLVSILLLSLFLFAVAASDHQFDDDNQSQRPWLQRCRQQIRSGERLRHCERLLTQP 60

Query: 61  RRVRQLLVSEEEEDAVEHLEECCRQLRGMDDPCRCEGLRQIVQRRRQQGRLQGIDVGQMM 120
           RR R LL +EEEEDAVEHLEECC+QL+GMDD CRCEGLRQIVQRRRQQG+LQG+DVGQM+
Sbjct: 61  RRFRLLLAAEEEEDAVEHLEECCQQLKGMDDACRCEGLRQIVQRRRQQGQLQGVDVGQML 120

Query: 121 ERATNLPSVCRLSQRRCDTRSS 139
           ERATNLPSVCRLS+RRCD RS+
Sbjct: 121 ERATNLPSVCRLSRRRCDLRST 142

BLAST of Lag0018533 vs. ExPASy Swiss-Prot
Match: P93198 (2S albumin seed storage protein (Fragment) OS=Juglans regia OX=51240 PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.2e-15
Identity = 51/139 (36.69%), Postives = 83/139 (59.71%), Query Frame = 0

Query: 9   LLIALFLFAAAAS--------ALHFDDDNQRPWLQRCRQQIEGPR-LRHCEQLLTRPRRV 68
           LL+AL   A AA+         +  D DN R   + CR+QI+  + L HC+  L +  R 
Sbjct: 3   LLVALLFVANAAAFRTTITTMEIDEDIDNPRRRGEGCREQIQRQQNLNHCQYYLRQQSRS 62

Query: 69  RQLLVSEEEEDAVEHLEECCRQLRGMDDPCRCEGLRQIVQRRRQQGRLQGIDVGQMMERA 128
                  +E++  +H  +CC+QL  MD+ C+CEGLRQ+V+R++QQ  L+G ++ +M++ A
Sbjct: 63  G----GYDEDNQRQHFRQCCQQLSQMDEQCQCEGLRQVVRRQQQQQGLRGEEMEEMVQSA 122

Query: 129 TNLPSVCRLSQRRCDTRSS 139
            +LP+ C +S +RC+ R S
Sbjct: 123 RDLPNECGISSQRCEIRRS 137

BLAST of Lag0018533 vs. ExPASy Swiss-Prot
Match: P0C8Y8 (2S sulfur-rich seed storage protein 2 OS=Bertholletia excelsa OX=3645 GN=BE2S2 PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.6e-12
Identity = 44/106 (41.51%), Postives = 63/106 (59.43%), Query Frame = 0

Query: 35  QRCRQQIE-GPRLRHCEQLLTRP------RRVRQLLVSEEEEDAVEHLEECCRQLRGMDD 94
           Q+CR+Q+E   +L HC   L +       +  R L   EE      HL+ECC QL  MD+
Sbjct: 45  QQCREQMERQQQLNHCRMYLRQQMEESPYQNPRPLRRGEE-----PHLDECCEQLERMDE 104

Query: 95  PCRCEGLRQIVQRRRQQGRLQGIDVGQMMERATNLPSVCRLSQRRC 134
            CRCEGLR +++R+R++  LQG  + ++M +A NL S C LS +RC
Sbjct: 105 MCRCEGLRMMLRRQREEMELQGEQMQRIMRKAENLLSRCNLSPQRC 145

BLAST of Lag0018533 vs. ExPASy Swiss-Prot
Match: P04403 (2S sulfur-rich seed storage protein 1 OS=Bertholletia excelsa OX=3645 GN=BE2S1 PE=1 SV=2)

HSP 1 Score: 67.8 bits (164), Expect = 1.1e-10
Identity = 41/120 (34.17%), Postives = 65/120 (54.17%), Query Frame = 0

Query: 16  FAAAASALHFDDDNQRPWLQRCRQQIEGPR-LRHCEQLLTRPRRVRQLLVSEEEEDAVEH 75
           F A  +    +++NQ    + CR+Q++  + L HC ++  R +       +        H
Sbjct: 23  FRATVTTTVVEEENQ----EECREQMQRQQMLSHC-RMYMRQQMEESPYQTMPRRGMEPH 82

Query: 76  LEECCRQLRGMDDPCRCEGLRQIVQRRRQQG-RLQGIDVGQMMERATNLPSVCRLSQRRC 134
           + ECC QL GMD+ CRCEGLR ++ R +Q+  + +G  + +MM  A N+PS C LS  RC
Sbjct: 83  MSECCEQLEGMDESCRCEGLRMMMMRMQQEEMQPRGEQMRRMMRLAENIPSRCNLSPMRC 137

BLAST of Lag0018533 vs. ExPASy Swiss-Prot
Match: P01089 (2S albumin OS=Ricinus communis OX=3988 PE=1 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 2.5e-10
Identity = 40/103 (38.83%), Postives = 57/103 (55.34%), Query Frame = 0

Query: 32  PWLQRCRQQI-EGPRLRHCEQLLTRPRRVRQLLVSEEEEDAVEHLEECCRQLRGMDDPCR 91
           P  Q CR QI E   LR C++ + +    +    S+ +E     L  CC  L+ M   CR
Sbjct: 157 PSQQGCRGQIQEQQNLRQCQEYIKQQVSGQGPRRSDNQE---RSLRGCCDHLKQMQSQCR 216

Query: 92  CEGLRQIVQRRRQQGRLQGIDVGQMMERATNLPSVCRLSQRRC 134
           CEGLRQ +++++ QG+LQG DV +    A NLPS+C +S   C
Sbjct: 217 CEGLRQAIEQQQSQGQLQGQDVFEAFRTAANLPSMCGVSPTEC 256

BLAST of Lag0018533 vs. ExPASy Swiss-Prot
Match: Q39649 (2S albumin OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 4.3e-10
Identity = 39/136 (28.68%), Postives = 74/136 (54.41%), Query Frame = 0

Query: 1   MATHPISILLIALFLFAAAASALHFDDDNQRPWLQRCRQQIEGPRLRHCEQLLTRPRR-- 60
           +A   +++L+   + +    + +   ++N++   +RCRQ      LR CEQ L +  R  
Sbjct: 8   IALFAVALLVADAYAYRTTITTVEV-EENRQGREERCRQMSAREELRSCEQYLRQQSRDV 67

Query: 61  VRQLLVSEEEEDAVEHLEECCRQLRGMDDPCRCEGLRQIVQRRRQQGRLQGIDVGQMMER 120
           ++   +           +ECCR+L+ +D+ CRC+ L +I +  ++Q R  G +  QM+++
Sbjct: 68  LQMRGIENPWRREGGSFDECCRELKNVDEECRCDMLEEIAREEQRQAR--GQEGRQMLQK 127

Query: 121 ATNLPSVCRLSQRRCD 135
           A NLPS+C +  +RCD
Sbjct: 128 ARNLPSMCGIRPQRCD 140