Lag0018520 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0018520 vs. NCBI nr
Match: XP_008463439.1 (PREDICTED: TBC1 domain family member 17 [Cucumis melo] >KAA0025500.1 TBC1 domain family member 17 [Cucumis melo var. makuwa] >TYK00364.1 TBC1 domain family member 17 [Cucumis melo var. makuwa])

HSP 1 Score: 862.8 bits (2228), Expect = 1.3e-246
Identity = 420/445 (94.38%), Postives = 430/445 (96.63%), Query Frame = 0

Query: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH 60
           MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH
Sbjct: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH 60

Query: 61  RGGIHPSIRGEVWEFLLGCYDPTSTFEEREEIRQRRRIQYATFKEDCRQMFPVIGSGRYI 120
           RGGIHPSIRGEVWEFLLGCYDP STFEERE IRQRRRI+YAT+KEDCRQMFPV+GSGRYI
Sbjct: 61  RGGIHPSIRGEVWEFLLGCYDPMSTFEEREAIRQRRRIEYATWKEDCRQMFPVVGSGRYI 120

Query: 121 TAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTGATDANSDGPRSNPVNNLEPVQDQKII 180
           TAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTG  D N DG RS P NNLE V+D KII
Sbjct: 121 TAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTGPADGNPDGSRSTPNNNLETVKDPKII 180

Query: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI 240
           QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI
Sbjct: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI 240

Query: 241 MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLSNLAAITQVIDPKLHQHLETLGG 300
           MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQL+NLAAITQVIDPKLHQHLETLGG
Sbjct: 241 MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLNNLAAITQVIDPKLHQHLETLGG 300

Query: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDMFAVYEETDMFSEKGEGSKGKA 360
           GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPD+  +YEE D+ +EKGEGSKGKA
Sbjct: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDLCTLYEEPDLGNEKGEGSKGKA 360

Query: 361 KSMRQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420
           KS+RQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG
Sbjct: 361 KSIRQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420

Query: 421 NLDAKKACTGAMKLHKKYLKKAKKA 446
           NLDAKKACTGAMKLHKKYLKKAKKA
Sbjct: 421 NLDAKKACTGAMKLHKKYLKKAKKA 445

BLAST of Lag0018520 vs. NCBI nr
Match: XP_038906214.1 (GTPase-activating protein GYP7 [Benincasa hispida])

HSP 1 Score: 860.1 bits (2221), Expect = 8.2e-246
Identity = 422/445 (94.83%), Postives = 431/445 (96.85%), Query Frame = 0

Query: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH 60
           MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH
Sbjct: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH 60

Query: 61  RGGIHPSIRGEVWEFLLGCYDPTSTFEEREEIRQRRRIQYATFKEDCRQMFPVIGSGRYI 120
           RGGIHPSIRGEVWEFLLGCYDP STFEERE IRQRRRI+YAT+KEDCRQMFPV+GSGRYI
Sbjct: 61  RGGIHPSIRGEVWEFLLGCYDPMSTFEEREAIRQRRRIEYATWKEDCRQMFPVVGSGRYI 120

Query: 121 TAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTGATDANSDGPRSNPVNNLEPVQDQKII 180
           TAPVITEDGQPIHDPLVLLETNP+ GPAVPQDTG  DAN DG RS   NNLE V+D KII
Sbjct: 121 TAPVITEDGQPIHDPLVLLETNPEKGPAVPQDTG-PDANLDGSRSTSSNNLETVKDPKII 180

Query: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI 240
           QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI
Sbjct: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI 240

Query: 241 MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLSNLAAITQVIDPKLHQHLETLGG 300
           MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQL+NLAAITQVIDPKLHQHLETLGG
Sbjct: 241 MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLNNLAAITQVIDPKLHQHLETLGG 300

Query: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDMFAVYEETDMFSEKGEGSKGKA 360
           GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPD+FA+YEE DM +EKGEGSKGKA
Sbjct: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDLFAMYEEPDMGNEKGEGSKGKA 360

Query: 361 KSMRQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420
           KSMRQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG
Sbjct: 361 KSMRQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420

Query: 421 NLDAKKACTGAMKLHKKYLKKAKKA 446
           NLDAKKACTGAMKLHKKYLKKAKKA
Sbjct: 421 NLDAKKACTGAMKLHKKYLKKAKKA 444

BLAST of Lag0018520 vs. NCBI nr
Match: XP_023521942.1 (GTPase-activating protein gyp7-like [Cucurbita pepo subsp. pepo] >XP_023551162.1 GTPase-activating protein gyp7-like [Cucurbita pepo subsp. pepo] >KAG6600767.1 TBC1 domain family member 15, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031404.1 TBC1 domain family member 15 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 852.8 bits (2202), Expect = 1.3e-243
Identity = 413/444 (93.02%), Postives = 428/444 (96.40%), Query Frame = 0

Query: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH 60
           MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDI+KTLNRIH
Sbjct: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDIAKTLNRIH 60

Query: 61  RGGIHPSIRGEVWEFLLGCYDPTSTFEEREEIRQRRRIQYATFKEDCRQMFPVIGSGRYI 120
           RGGIHPSIRGEVWEFLLGCYDP ST EEREEIRQRRRIQYAT+KEDCRQMFPVIGSGRYI
Sbjct: 61  RGGIHPSIRGEVWEFLLGCYDPMSTIEEREEIRQRRRIQYATWKEDCRQMFPVIGSGRYI 120

Query: 121 TAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTGATDANSDGPRSNPVNNLEPVQDQKII 180
           TAPVITEDGQPI DPLVLLE NP++GP VPQDTG  DA SDG + N  +N+EPV DQKII
Sbjct: 121 TAPVITEDGQPIQDPLVLLEANPESGPLVPQDTGTADAESDGSQPNSCSNMEPVTDQKII 180

Query: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI 240
           QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKD+GYCQGMSDLCSPMI
Sbjct: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDIGYCQGMSDLCSPMI 240

Query: 241 MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLSNLAAITQVIDPKLHQHLETLGG 300
           MLLEDEGD+FWCFERLMRRLRGNFRCTDSSVGVETQL+NLAAITQVIDPKLHQHLETLGG
Sbjct: 241 MLLEDEGDSFWCFERLMRRLRGNFRCTDSSVGVETQLNNLAAITQVIDPKLHQHLETLGG 300

Query: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDMFAVYEETDMFSEKGEGSKGKA 360
           GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPD+FA+YEE +M +EKGEGSKGK 
Sbjct: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDLFALYEEPEMCNEKGEGSKGKE 360

Query: 361 KSMRQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420
           KSMRQCGKYERENLKAKN+QAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG
Sbjct: 361 KSMRQCGKYERENLKAKNAQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420

Query: 421 NLDAKKACTGAMKLHKKYLKKAKK 445
           NLDAKKACTGAMKLHKKYLKKAKK
Sbjct: 421 NLDAKKACTGAMKLHKKYLKKAKK 444

BLAST of Lag0018520 vs. NCBI nr
Match: XP_022942457.1 (GTPase-activating protein gyp7-like [Cucurbita moschata])

HSP 1 Score: 851.3 bits (2198), Expect = 3.8e-243
Identity = 412/444 (92.79%), Postives = 428/444 (96.40%), Query Frame = 0

Query: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH 60
           MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDI+KTLNRIH
Sbjct: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDIAKTLNRIH 60

Query: 61  RGGIHPSIRGEVWEFLLGCYDPTSTFEEREEIRQRRRIQYATFKEDCRQMFPVIGSGRYI 120
           RGGIHPSIRGEVWEFLLGCYDP ST EEREEIRQRRRIQYAT+KEDCRQMFPVIGSGRYI
Sbjct: 61  RGGIHPSIRGEVWEFLLGCYDPMSTIEEREEIRQRRRIQYATWKEDCRQMFPVIGSGRYI 120

Query: 121 TAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTGATDANSDGPRSNPVNNLEPVQDQKII 180
           TAPVITEDGQPI DPLVLLE NP++GP VPQDTG  DA SDG + N  +N+EPV DQKII
Sbjct: 121 TAPVITEDGQPIQDPLVLLEANPESGPLVPQDTGTADAESDGSQPNSCSNMEPVTDQKII 180

Query: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI 240
           QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKD+GYCQGMSDLCSPMI
Sbjct: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDIGYCQGMSDLCSPMI 240

Query: 241 MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLSNLAAITQVIDPKLHQHLETLGG 300
           MLLEDEGD+FWCFERLMRRLRGNFRCTDSSVGVETQL+NLAAITQVIDPKLHQHLETLGG
Sbjct: 241 MLLEDEGDSFWCFERLMRRLRGNFRCTDSSVGVETQLNNLAAITQVIDPKLHQHLETLGG 300

Query: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDMFAVYEETDMFSEKGEGSKGKA 360
           GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPD+FA+YEE ++ +EKGEGSKGK 
Sbjct: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDLFALYEEPEICNEKGEGSKGKE 360

Query: 361 KSMRQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420
           KSMRQCGKYERENLKAKN+QAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG
Sbjct: 361 KSMRQCGKYERENLKAKNAQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420

Query: 421 NLDAKKACTGAMKLHKKYLKKAKK 445
           NLDAKKACTGAMKLHKKYLKKAKK
Sbjct: 421 NLDAKKACTGAMKLHKKYLKKAKK 444

BLAST of Lag0018520 vs. NCBI nr
Match: XP_004152917.1 (GTPase-activating protein gyp7 [Cucumis sativus] >KAE8650139.1 hypothetical protein Csa_009471 [Cucumis sativus])

HSP 1 Score: 846.7 bits (2186), Expect = 9.4e-242
Identity = 414/445 (93.03%), Postives = 426/445 (95.73%), Query Frame = 0

Query: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH 60
           MW + GAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH
Sbjct: 1   MW-NTGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH 60

Query: 61  RGGIHPSIRGEVWEFLLGCYDPTSTFEEREEIRQRRRIQYATFKEDCRQMFPVIGSGRYI 120
           RGGIHPSIRGEVWEFLLGCYDP STFEERE IRQRRRI+YAT+KEDCRQMFPV+GSGRYI
Sbjct: 61  RGGIHPSIRGEVWEFLLGCYDPMSTFEEREAIRQRRRIEYATWKEDCRQMFPVVGSGRYI 120

Query: 121 TAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTGATDANSDGPRSNPVNNLEPVQDQKII 180
           TAPVITEDGQPIHDPLVLLETNPD GPAVPQDT   D N DG RS P NNLE V+D KII
Sbjct: 121 TAPVITEDGQPIHDPLVLLETNPDKGPAVPQDTSTADGNPDGSRSTPNNNLETVKDPKII 180

Query: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI 240
           QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI
Sbjct: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI 240

Query: 241 MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLSNLAAITQVIDPKLHQHLETLGG 300
           MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQL+NLAAITQVIDPKLHQHLETLGG
Sbjct: 241 MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLNNLAAITQVIDPKLHQHLETLGG 300

Query: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDMFAVYEETDMFSEKGEGSKGKA 360
           GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPD+  +YEE D+ +EKGEGSKGKA
Sbjct: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDLCVLYEEPDIGNEKGEGSKGKA 360

Query: 361 KSMRQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420
           KS+RQCGKYERENLKAKNS+APLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG
Sbjct: 361 KSIRQCGKYERENLKAKNSEAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420

Query: 421 NLDAKKACTGAMKLHKKYLKKAKKA 446
           NLDAKKACTGAMKLHKKYLKKAKKA
Sbjct: 421 NLDAKKACTGAMKLHKKYLKKAKKA 444

BLAST of Lag0018520 vs. ExPASy Swiss-Prot
Match: Q94BY9 (Rab GTPase-activating protein 22 OS=Arabidopsis thaliana OX=3702 GN=RABGAP22 PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 7.6e-37
Identity = 135/473 (28.54%), Postives = 205/473 (43.34%), Query Frame = 0

Query: 35  LSVRKWQAAFNPEGQLDISKT--LNRIHRGGIHPSIRGEVWEFLLGCYDPTSTFEEREEI 94
           L+  +W++ F PEG+L       L ++   G+ PSIR EVW FLLG YD  ST EERE +
Sbjct: 96  LTPHQWRSLFTPEGKLRDGGVGFLKKVRSRGVDPSIRAEVWLFLLGVYDLNSTSEEREAV 155

Query: 95  RQRRRIQYATFKEDCRQMFPVIGSGRYITAPVITE-----------DGQPIHDPLVLLET 154
           + ++R +Y   +  C QM    G+G       +             D   I  P+    T
Sbjct: 156 KTQKRKEYEKLQRRC-QMLLKCGNGSTDNLEELPSDEANSQCVRFVDDYKITGPM----T 215

Query: 155 NPDNGPAVPQDTGATDANSDG-----------------PRSNPVNNLEP-----VQDQKI 214
           + D   A+  D+  TD+  D                     N  NN E      V     
Sbjct: 216 SQDVVSALNTDSSDTDSCEDNEDVLLLSSFAHSDEKKPEEDNSNNNSEENSSLLVAAASE 275

Query: 215 IQWMLTLHQ--------IGLDVVRTD---------RTLVFYEKQENL------------- 274
           +Q  + +H+        I LD +R D          T +   K   L             
Sbjct: 276 VQVEVAVHEDFSTWQRIIRLDALRADSEWANYSPYSTAITESKARRLAESVGLKDYDHLE 335

Query: 275 -------SKLWDILAVYAWIDKDVGYCQGMSDLCSPMIMLLEDEGDAFWCFERLMRRLRG 334
                  ++L  IL  YA  D ++GYCQGMSDL SP++ ++ ++ +AFWCF   M++ R 
Sbjct: 336 SCRLYHAARLVAILEAYAMYDPEIGYCQGMSDLLSPILAVISEDHEAFWCFVGFMKKARH 395

Query: 335 NFRCTDSSVGVETQLSNLAAITQVIDPKLHQHLETLGGGDYLFAFRMLMVLFRREFSFCD 394
           NFR  ++  G++ QLS ++ I +  D +L++HLE L   D  F +RM++V+FRRE SF  
Sbjct: 396 NFRLDEA--GIQRQLSIVSKIIKNKDSQLYKHLENLQAEDCSFVYRMVLVMFRRELSFEQ 455

Query: 395 SLYLWEMMWALEYDPDMFAVYEETDMFSEKGEGSKGKAKSMRQCGKYERENLKAKNSQAP 435
           +L LWE+MWA              D  + +    K     +RQ              QAP
Sbjct: 456 TLCLWEVMWA--------------DQAAIRAGVGKSPWSRIRQ--------------QAP 515

BLAST of Lag0018520 vs. ExPASy Swiss-Prot
Match: Q9CXF4 (TBC1 domain family member 15 OS=Mus musculus OX=10090 GN=Tbc1d15 PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.2e-34
Identity = 92/306 (30.07%), Postives = 148/306 (48.37%), Query Frame = 0

Query: 29  IRAGKTLSVRKWQAAFNPEGQL-DISKTLNRIHRGGIHPSIRGEVWEFLLGCYDPTSTFE 88
           ++  + +S+ +W  + +PEG+L  +     +I RGG+  S+R + W+FLLG +   ST E
Sbjct: 294 VQRREPVSLEEWNKSLDPEGRLVAVESMKQKIFRGGLSHSLRKQAWKFLLGYFPWDSTKE 353

Query: 89  EREEIRQRRRIQYATFKEDCRQMFPVIGSGRYITAPVITEDGQPIHDPLVLLETNPDNGP 148
           ER ++++++  +Y   K   +                                       
Sbjct: 354 ERTQLQKQKTDEYFRMKLQWKS-------------------------------------- 413

Query: 149 AVPQDTGATDANSDGPRSNPVNNLEPVQDQKIIQWMLTLHQIGLDVVRTDRTLVFYEKQE 208
                               V+  +  ++ ++  +   + +   DV RTDRT  FYE Q+
Sbjct: 414 --------------------VSEAQEKRNSRLRDYRSLIEK---DVNRTDRTNKFYEGQD 473

Query: 209 N--LSKLWDILAVYAWIDKDVGYCQGMSDLCSPMIMLLEDEGDAFWCFERLMRRLRGNFR 268
           N  L  L DIL  Y   D D+GY QGMSDL SP++ ++E+E DAFWCF   M ++  NF 
Sbjct: 474 NPGLILLHDILMTYCMYDFDLGYVQGMSDLLSPLLYVMENEVDAFWCFASYMDQMHQNFE 533

Query: 269 CTDSSVGVETQLSNLAAITQVIDPKLHQHLETLGGGDYLFAFRMLMVLFRREFSFCDSLY 328
             +   G++TQL  L+ + +++D     +LE+   G   F FR L++ F+REFSF D L 
Sbjct: 534 --EQMQGMKTQLIQLSTLLRLLDSGFCSYLESQDSGYLYFCFRWLLIRFKREFSFLDILR 536

Query: 329 LWEMMW 332
           LWE+MW
Sbjct: 594 LWEVMW 536

BLAST of Lag0018520 vs. ExPASy Swiss-Prot
Match: Q8TC07 (TBC1 domain family member 15 OS=Homo sapiens OX=9606 GN=TBC1D15 PE=1 SV=2)

HSP 1 Score: 141.7 bits (356), Expect = 1.9e-32
Identity = 93/306 (30.39%), Postives = 145/306 (47.39%), Query Frame = 0

Query: 29  IRAGKTLSVRKWQAAFNPEGQ-LDISKTLNRIHRGGIHPSIRGEVWEFLLGCYDPTSTFE 88
           ++  + +S+ +W    + EG+ L++      I RGG+  ++R + W+FLLG +   ST E
Sbjct: 311 VQRREPVSLEEWTKNIDSEGRILNVDNMKQMIFRGGLSHALRKQAWKFLLGYFPWDSTKE 370

Query: 89  EREEIRQRRRIQYATFKEDCRQMFPVIGSGRYITAPVITEDGQPIHDPLVLLETNPDNGP 148
           ER ++++++  +Y   K   +                                       
Sbjct: 371 ERTQLQKQKTDEYFRMKLQWK--------------------------------------- 430

Query: 149 AVPQDTGATDANSDGPRSNPVNNLEPVQDQKIIQWMLTLHQIGLDVVRTDRTLVFYEKQE 208
           ++ Q+    ++     RS                       I  DV RTDRT  FYE Q+
Sbjct: 431 SISQEQEKRNSRLRDYRS----------------------LIEKDVNRTDRTNKFYEGQD 490

Query: 209 N--LSKLWDILAVYAWIDKDVGYCQGMSDLCSPMIMLLEDEGDAFWCFERLMRRLRGNFR 268
           N  L  L DIL  Y   D D+GY QGMSDL SP++ ++E+E DAFWCF   M ++  NF 
Sbjct: 491 NPGLILLHDILMTYCMYDFDLGYVQGMSDLLSPLLYVMENEVDAFWCFASYMDQMHQNFE 550

Query: 269 CTDSSVGVETQLSNLAAITQVIDPKLHQHLETLGGGDYLFAFRMLMVLFRREFSFCDSLY 328
             +   G++TQL  L+ + +++D     +LE+   G   F FR L++ F+REFSF D L 
Sbjct: 551 --EQMQGMKTQLIQLSTLLRLLDSGFCSYLESQDSGYLYFCFRWLLIRFKREFSFLDILR 553

Query: 329 LWEMMW 332
           LWE+MW
Sbjct: 611 LWEVMW 553

BLAST of Lag0018520 vs. ExPASy Swiss-Prot
Match: Q9HA65 (TBC1 domain family member 17 OS=Homo sapiens OX=9606 GN=TBC1D17 PE=1 SV=2)

HSP 1 Score: 140.6 bits (353), Expect = 4.3e-32
Identity = 94/306 (30.72%), Postives = 137/306 (44.77%), Query Frame = 0

Query: 29  IRAGKTLSVRKWQAAFNPEGQL-DISKTLNRIHRGGIHPSIRGEVWEFLLGCYDPTSTFE 88
           +  G  ++  +W     PEG+L  + +  NRI  GG+ PS+R E W+FLLG      T E
Sbjct: 275 VERGPPVTEEEWARHVGPEGRLQQVPELKNRIFSGGLSPSLRREAWKFLLGYLSWEGTAE 334

Query: 89  EREEIRQRRRIQYATFKEDCRQMFPVIGSGRYITAPVITEDGQPIHDPLVLLETNPDNGP 148
           E +   +++  +Y   K   + + P                                   
Sbjct: 335 EHKAHIRKKTDEYFRMKLQWKSVSP----------------------------------- 394

Query: 149 AVPQDTGATDANSDGPRSNPVNNLEPVQDQKIIQWMLTLHQIGLDVVRTDRTLVFYEKQE 208
                                   E  +   ++    +L  I  DV RTDRT  FYE  E
Sbjct: 395 ------------------------EQERRNSLLHGYRSL--IERDVSRTDRTNKFYEGPE 454

Query: 209 N--LSKLWDILAVYAWIDKDVGYCQGMSDLCSPMIMLLEDEGDAFWCFERLMRRLRGNFR 268
           N  L  L DIL  Y     D+GY QGMSDL SP++ ++++E DAFWCF   M  ++GNF 
Sbjct: 455 NPGLGLLNDILLTYCMYHFDLGYVQGMSDLLSPILYVIQNEVDAFWCFCGFMELVQGNFE 514

Query: 269 CTDSSVGVETQLSNLAAITQVIDPKLHQHLETLGGGDYLFAFRMLMVLFRREFSFCDSLY 328
             +S   ++ QL  L  + +V+DP L   L++   G   F FR L++ F+REF F D L 
Sbjct: 515 --ESQETMKRQLGRLLLLLRVLDPLLCDFLDSQDSGSLCFCFRWLLIWFKREFPFPDVLR 517

Query: 329 LWEMMW 332
           LWE++W
Sbjct: 575 LWEVLW 517

BLAST of Lag0018520 vs. ExPASy Swiss-Prot
Match: Q8BYH7 (TBC1 domain family member 17 OS=Mus musculus OX=10090 GN=Tbc1d17 PE=1 SV=2)

HSP 1 Score: 132.5 bits (332), Expect = 1.2e-29
Identity = 93/296 (31.42%), Postives = 133/296 (44.93%), Query Frame = 0

Query: 39  KWQAAFNPEGQL-DISKTLNRIHRGGIHPSIRGEVWEFLLGCYDPTSTFEEREEIRQRRR 98
           +W     PEG+L ++ +  NRI  GG+ P +R E W+FLLG     S+ EE +   +++ 
Sbjct: 285 EWNRYVGPEGRLQNVPELKNRIFSGGLSPGLRREAWKFLLGYLSWESSAEEHKAHVRKKT 344

Query: 99  IQYATFKEDCRQMFPVIGSGRYITAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTGATD 158
            +Y   K   +                                       +V  +    +
Sbjct: 345 DEYFRMKLQWK---------------------------------------SVSAEQERRN 404

Query: 159 ANSDGPRSNPVNNLEPVQDQKIIQWMLTLHQIGLDVVRTDRTLVFYEKQEN--LSKLWDI 218
           +   G RS                       I  DV RTDRT  FYE  EN  LS L DI
Sbjct: 405 SLLHGYRS----------------------LIERDVSRTDRTNKFYEGPENPGLSLLHDI 464

Query: 219 LAVYAWIDKDVGYCQGMSDLCSPMIMLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVET 278
           L  Y     D+GY QGMSDL SP++ ++++E DAFWCF   M  + GNF   +S   ++ 
Sbjct: 465 LLTYCMYHFDLGYVQGMSDLLSPILFVVQNEVDAFWCFCGFMELVHGNFE--ESQETMKR 517

Query: 279 QLSNLAAITQVIDPKLHQHLETLGGGDYLFAFRMLMVLFRREFSFCDSLYLWEMMW 332
           QL  L  + +V+D  L   L++   G   F FR L++ F+REF F D L LWE++W
Sbjct: 525 QLGQLLLLLRVLDQPLCDFLDSQDSGSLCFCFRWLLIWFKREFPFPDVLRLWEVLW 517

BLAST of Lag0018520 vs. ExPASy TrEMBL
Match: A0A5D3BM65 (TBC1 domain family member 17 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1112G00280 PE=4 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 6.1e-247
Identity = 420/445 (94.38%), Postives = 430/445 (96.63%), Query Frame = 0

Query: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH 60
           MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH
Sbjct: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH 60

Query: 61  RGGIHPSIRGEVWEFLLGCYDPTSTFEEREEIRQRRRIQYATFKEDCRQMFPVIGSGRYI 120
           RGGIHPSIRGEVWEFLLGCYDP STFEERE IRQRRRI+YAT+KEDCRQMFPV+GSGRYI
Sbjct: 61  RGGIHPSIRGEVWEFLLGCYDPMSTFEEREAIRQRRRIEYATWKEDCRQMFPVVGSGRYI 120

Query: 121 TAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTGATDANSDGPRSNPVNNLEPVQDQKII 180
           TAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTG  D N DG RS P NNLE V+D KII
Sbjct: 121 TAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTGPADGNPDGSRSTPNNNLETVKDPKII 180

Query: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI 240
           QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI
Sbjct: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI 240

Query: 241 MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLSNLAAITQVIDPKLHQHLETLGG 300
           MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQL+NLAAITQVIDPKLHQHLETLGG
Sbjct: 241 MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLNNLAAITQVIDPKLHQHLETLGG 300

Query: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDMFAVYEETDMFSEKGEGSKGKA 360
           GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPD+  +YEE D+ +EKGEGSKGKA
Sbjct: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDLCTLYEEPDLGNEKGEGSKGKA 360

Query: 361 KSMRQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420
           KS+RQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG
Sbjct: 361 KSIRQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420

Query: 421 NLDAKKACTGAMKLHKKYLKKAKKA 446
           NLDAKKACTGAMKLHKKYLKKAKKA
Sbjct: 421 NLDAKKACTGAMKLHKKYLKKAKKA 445

BLAST of Lag0018520 vs. ExPASy TrEMBL
Match: A0A1S3CJA4 (TBC1 domain family member 17 OS=Cucumis melo OX=3656 GN=LOC103501604 PE=4 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 6.1e-247
Identity = 420/445 (94.38%), Postives = 430/445 (96.63%), Query Frame = 0

Query: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH 60
           MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH
Sbjct: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH 60

Query: 61  RGGIHPSIRGEVWEFLLGCYDPTSTFEEREEIRQRRRIQYATFKEDCRQMFPVIGSGRYI 120
           RGGIHPSIRGEVWEFLLGCYDP STFEERE IRQRRRI+YAT+KEDCRQMFPV+GSGRYI
Sbjct: 61  RGGIHPSIRGEVWEFLLGCYDPMSTFEEREAIRQRRRIEYATWKEDCRQMFPVVGSGRYI 120

Query: 121 TAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTGATDANSDGPRSNPVNNLEPVQDQKII 180
           TAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTG  D N DG RS P NNLE V+D KII
Sbjct: 121 TAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTGPADGNPDGSRSTPNNNLETVKDPKII 180

Query: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI 240
           QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI
Sbjct: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI 240

Query: 241 MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLSNLAAITQVIDPKLHQHLETLGG 300
           MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQL+NLAAITQVIDPKLHQHLETLGG
Sbjct: 241 MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLNNLAAITQVIDPKLHQHLETLGG 300

Query: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDMFAVYEETDMFSEKGEGSKGKA 360
           GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPD+  +YEE D+ +EKGEGSKGKA
Sbjct: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDLCTLYEEPDLGNEKGEGSKGKA 360

Query: 361 KSMRQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420
           KS+RQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG
Sbjct: 361 KSIRQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420

Query: 421 NLDAKKACTGAMKLHKKYLKKAKKA 446
           NLDAKKACTGAMKLHKKYLKKAKKA
Sbjct: 421 NLDAKKACTGAMKLHKKYLKKAKKA 445

BLAST of Lag0018520 vs. ExPASy TrEMBL
Match: A0A6J1FQA4 (GTPase-activating protein gyp7-like OS=Cucurbita moschata OX=3662 GN=LOC111447490 PE=4 SV=1)

HSP 1 Score: 851.3 bits (2198), Expect = 1.8e-243
Identity = 412/444 (92.79%), Postives = 428/444 (96.40%), Query Frame = 0

Query: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH 60
           MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDI+KTLNRIH
Sbjct: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDIAKTLNRIH 60

Query: 61  RGGIHPSIRGEVWEFLLGCYDPTSTFEEREEIRQRRRIQYATFKEDCRQMFPVIGSGRYI 120
           RGGIHPSIRGEVWEFLLGCYDP ST EEREEIRQRRRIQYAT+KEDCRQMFPVIGSGRYI
Sbjct: 61  RGGIHPSIRGEVWEFLLGCYDPMSTIEEREEIRQRRRIQYATWKEDCRQMFPVIGSGRYI 120

Query: 121 TAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTGATDANSDGPRSNPVNNLEPVQDQKII 180
           TAPVITEDGQPI DPLVLLE NP++GP VPQDTG  DA SDG + N  +N+EPV DQKII
Sbjct: 121 TAPVITEDGQPIQDPLVLLEANPESGPLVPQDTGTADAESDGSQPNSCSNMEPVTDQKII 180

Query: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI 240
           QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKD+GYCQGMSDLCSPMI
Sbjct: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDIGYCQGMSDLCSPMI 240

Query: 241 MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLSNLAAITQVIDPKLHQHLETLGG 300
           MLLEDEGD+FWCFERLMRRLRGNFRCTDSSVGVETQL+NLAAITQVIDPKLHQHLETLGG
Sbjct: 241 MLLEDEGDSFWCFERLMRRLRGNFRCTDSSVGVETQLNNLAAITQVIDPKLHQHLETLGG 300

Query: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDMFAVYEETDMFSEKGEGSKGKA 360
           GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPD+FA+YEE ++ +EKGEGSKGK 
Sbjct: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDLFALYEEPEICNEKGEGSKGKE 360

Query: 361 KSMRQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420
           KSMRQCGKYERENLKAKN+QAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG
Sbjct: 361 KSMRQCGKYERENLKAKNAQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420

Query: 421 NLDAKKACTGAMKLHKKYLKKAKK 445
           NLDAKKACTGAMKLHKKYLKKAKK
Sbjct: 421 NLDAKKACTGAMKLHKKYLKKAKK 444

BLAST of Lag0018520 vs. ExPASy TrEMBL
Match: A0A6J1DNU7 (GTPase-activating protein GYP7 OS=Momordica charantia OX=3673 GN=LOC111022125 PE=4 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 1.0e-241
Identity = 410/444 (92.34%), Postives = 427/444 (96.17%), Query Frame = 0

Query: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH 60
           MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAF PEG+LDISKTLNRIH
Sbjct: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFTPEGELDISKTLNRIH 60

Query: 61  RGGIHPSIRGEVWEFLLGCYDPTSTFEEREEIRQRRRIQYATFKEDCRQMFPVIGSGRYI 120
           RGGIHP+IRGEVWEFLLGCYDP STFEERE IRQRRRI+YAT+KEDCRQMFPV+GSGRYI
Sbjct: 61  RGGIHPAIRGEVWEFLLGCYDPKSTFEEREAIRQRRRIEYATWKEDCRQMFPVVGSGRYI 120

Query: 121 TAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTGATDANSDGPRSNPVNNLEPVQDQKII 180
           TAPVITEDGQPIHDPLVLLETNP+ GPAVPQD    D NSDG R+   +N+E V+D+KII
Sbjct: 121 TAPVITEDGQPIHDPLVLLETNPETGPAVPQDPSTGDVNSDGSRAT-ASNMETVKDEKII 180

Query: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI 240
           QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI
Sbjct: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI 240

Query: 241 MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLSNLAAITQVIDPKLHQHLETLGG 300
           MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQL+NLAAITQVIDPKLHQHLETLGG
Sbjct: 241 MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLNNLAAITQVIDPKLHQHLETLGG 300

Query: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDMFAVYEETDMFSEKGEGSKGKA 360
           GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPD+FA+YEE D+ +EK EGSKGKA
Sbjct: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDLFAIYEEPDLLNEKAEGSKGKA 360

Query: 361 KSMRQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420
           KS+RQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG
Sbjct: 361 KSIRQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420

Query: 421 NLDAKKACTGAMKLHKKYLKKAKK 445
           NLDAKKACTGAMKLHKKYLKKAKK
Sbjct: 421 NLDAKKACTGAMKLHKKYLKKAKK 443

BLAST of Lag0018520 vs. ExPASy TrEMBL
Match: A0A6J1ILF0 (GTPase-activating protein GYP7-like OS=Cucurbita maxima OX=3661 GN=LOC111476657 PE=4 SV=1)

HSP 1 Score: 845.1 bits (2182), Expect = 1.3e-241
Identity = 409/444 (92.12%), Postives = 427/444 (96.17%), Query Frame = 0

Query: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH 60
           MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFN EGQLDI+K+LNRIH
Sbjct: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNSEGQLDIAKSLNRIH 60

Query: 61  RGGIHPSIRGEVWEFLLGCYDPTSTFEEREEIRQRRRIQYATFKEDCRQMFPVIGSGRYI 120
           RGGIHPSIRGEVWEFLLGCYDP ST EEREEIRQRRRIQYAT+KEDCRQMFPVIGSGRYI
Sbjct: 61  RGGIHPSIRGEVWEFLLGCYDPMSTIEEREEIRQRRRIQYATWKEDCRQMFPVIGSGRYI 120

Query: 121 TAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTGATDANSDGPRSNPVNNLEPVQDQKII 180
           TAPVITEDGQPI DPLVLLE NP++GP VPQDTG  DA S+G + N  +N+EPV DQKII
Sbjct: 121 TAPVITEDGQPIQDPLVLLEANPESGPVVPQDTGTADAESNGSQPNSCSNMEPVTDQKII 180

Query: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI 240
           QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKD+GYCQGMSDLCSPMI
Sbjct: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDIGYCQGMSDLCSPMI 240

Query: 241 MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLSNLAAITQVIDPKLHQHLETLGG 300
           MLLEDEGD+FWCFERLMRRLRGNFRCTDSSVGVETQL+NLAAITQVIDPKLHQHLETLGG
Sbjct: 241 MLLEDEGDSFWCFERLMRRLRGNFRCTDSSVGVETQLNNLAAITQVIDPKLHQHLETLGG 300

Query: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDMFAVYEETDMFSEKGEGSKGKA 360
           GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPD+FA+YEE ++ +EKGEGSKGK 
Sbjct: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDLFALYEEPEICNEKGEGSKGKE 360

Query: 361 KSMRQCGKYERENLKAKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420
           KSMRQCGKYERENLKAKN+QAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG
Sbjct: 361 KSMRQCGKYERENLKAKNAQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDMTG 420

Query: 421 NLDAKKACTGAMKLHKKYLKKAKK 445
           NLDAKKACTGAMKLHKKYLKKAKK
Sbjct: 421 NLDAKKACTGAMKLHKKYLKKAKK 444

BLAST of Lag0018520 vs. TAIR 10
Match: AT5G54780.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 682.2 bits (1759), Expect = 2.8e-196
Identity = 342/448 (76.34%), Postives = 380/448 (84.82%), Query Frame = 0

Query: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH 60
           MW  A  PADS+Y +RPECTDVP T+FKI+ GKTLSVRKWQAAF  EG LDI KTL+RI 
Sbjct: 1   MWGAAAEPADSYYLIRPECTDVPNTKFKIKPGKTLSVRKWQAAFTTEGFLDIGKTLSRIQ 60

Query: 61  RGGIHPSIRGEVWEFLLGCYDPTSTFEEREEIRQRRRIQYATFKEDCRQMFPVIGSGRYI 120
           RGGIHPSIRGEVWEFLLGCYDP STFEERE+IRQRRR+QYA++KE+C+QMFPVIGSG +I
Sbjct: 61  RGGIHPSIRGEVWEFLLGCYDPKSTFEEREQIRQRRRLQYASWKEECKQMFPVIGSGGFI 120

Query: 121 TAPVITEDGQPIHDPLVLLETN-PDNGPAVPQDTGATDANSDGPRSNPVNNLEPVQDQKI 180
           TAPVIT  G+PI+DP+VL ETN   NG    +D       S GP            DQK+
Sbjct: 121 TAPVITNKGEPIYDPIVLQETNLGANGSDFFKDLA-----SRGP-----------LDQKV 180

Query: 181 IQWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPM 240
           IQW+LTLHQIGLDV RTDRTLVFYEK+ENLSKLWDILA+YAWID DVGYCQGMSDLCSPM
Sbjct: 181 IQWLLTLHQIGLDVNRTDRTLVFYEKKENLSKLWDILALYAWIDNDVGYCQGMSDLCSPM 240

Query: 241 IMLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLSNLAAITQVIDPKLHQHLETLG 300
           IMLLEDE DAFWCFERLMRRLRGNFR T  SVGVE QL++LA+ITQ+IDPKLH HLE LG
Sbjct: 241 IMLLEDEADAFWCFERLMRRLRGNFRDTGRSVGVEAQLTHLASITQIIDPKLHHHLEKLG 300

Query: 301 GGDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDMFAVYEETDMFSEKGEG-SKG 360
           GGDYLFA RM+MV FRREFSFCDSLYLWEMMWALEYDP+M+++YEE     E+ EG SKG
Sbjct: 301 GGDYLFAIRMIMVQFRREFSFCDSLYLWEMMWALEYDPEMYSLYEEPQFEGERIEGSSKG 360

Query: 361 KAKSMRQCGKYERENLK--AKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILN 420
           K KS+ QCGKYEREN+K   K+++ PLPISVFLVASVLKDKS+KL+TEARGLDDVVKILN
Sbjct: 361 KPKSINQCGKYERENMKNGGKSAEGPLPISVFLVASVLKDKSSKLMTEARGLDDVVKILN 420

Query: 421 DMTGNLDAKKACTGAMKLHKKYLKKAKK 445
           D+TGNLDAKKACTGAMKLHKKYLKKAKK
Sbjct: 421 DITGNLDAKKACTGAMKLHKKYLKKAKK 432

BLAST of Lag0018520 vs. TAIR 10
Match: AT4G27100.2 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 675.2 bits (1741), Expect = 3.5e-194
Identity = 333/446 (74.66%), Postives = 373/446 (83.63%), Query Frame = 0

Query: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH 60
           MW  AG PADS+Y+VRPECTDVPKTRFKI+ GKTLSVRKWQA F  EG L I KTL RI 
Sbjct: 1   MWGGAGEPADSYYQVRPECTDVPKTRFKIKPGKTLSVRKWQAVFVQEGSLHIGKTLRRIR 60

Query: 61  RGGIHPSIRGEVWEFLLGCYDPTSTFEEREEIRQRRRIQYATFKEDCRQMFPVIGSGRYI 120
           RGGIHPSIRGEVWEFLLGCYDP STFEERE+IRQRRR+QYA++KE+C+QMFPVIGSGR+ 
Sbjct: 61  RGGIHPSIRGEVWEFLLGCYDPMSTFEEREQIRQRRRLQYASWKEECKQMFPVIGSGRFT 120

Query: 121 TAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTGATDANSDGPRSNPVNNLEPVQDQKII 180
           TAPVITE+GQP +DPLVL E N                NS+G             D+KII
Sbjct: 121 TAPVITENGQPNYDPLVLQEIN-------------LGTNSNGSVFFKELTSRGPLDKKII 180

Query: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI 240
           QW+LTLHQIGLDV RTDR LVFYEK+ENLSKLWDIL+VYAWID DVGYCQGMSDLCSPMI
Sbjct: 181 QWLLTLHQIGLDVNRTDRALVFYEKKENLSKLWDILSVYAWIDNDVGYCQGMSDLCSPMI 240

Query: 241 MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLSNLAAITQVIDPKLHQHLETLGG 300
           +LLEDE DAFWCFERLMRRLRGNFR T  SVGVE QL++L++ITQV+DPKLHQHL+ LGG
Sbjct: 241 ILLEDEADAFWCFERLMRRLRGNFRSTGRSVGVEAQLTHLSSITQVVDPKLHQHLDKLGG 300

Query: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDMFAVYEETDMFSEKGEGSKGKA 360
           GDYLFA RMLMV FRREFSFCDSLYLWEMMWALEYDPD+F VYE     +EK EG KGK 
Sbjct: 301 GDYLFAIRMLMVQFRREFSFCDSLYLWEMMWALEYDPDLFYVYEAHQCGNEKTEGLKGKP 360

Query: 361 KSMRQCGKYERENLK--AKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDM 420
           KS++QCGKYER+N++   K+++ PLPISVFLVASVLKDKS KL+TEARGLDDVVKILND 
Sbjct: 361 KSIKQCGKYERQNMRNGGKSAEGPLPISVFLVASVLKDKSYKLMTEARGLDDVVKILNDT 420

Query: 421 TGNLDAKKACTGAMKLHKKYLKKAKK 445
           TGNLDAKK C+GA+K+HK+YL+KAKK
Sbjct: 421 TGNLDAKKTCSGAIKIHKRYLRKAKK 433

BLAST of Lag0018520 vs. TAIR 10
Match: AT4G27100.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 671.0 bits (1730), Expect = 6.6e-193
Identity = 330/443 (74.49%), Postives = 370/443 (83.52%), Query Frame = 0

Query: 1   MWRDAGAPADSFYEVRPECTDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRIH 60
           MW  AG PADS+Y+VRPECTDVPKTRFKI+ GKTLSVRKWQA F  EG L I KTL RI 
Sbjct: 1   MWGGAGEPADSYYQVRPECTDVPKTRFKIKPGKTLSVRKWQAVFVQEGSLHIGKTLRRIR 60

Query: 61  RGGIHPSIRGEVWEFLLGCYDPTSTFEEREEIRQRRRIQYATFKEDCRQMFPVIGSGRYI 120
           RGGIHPSIRGEVWEFLLGCYDP STFEERE+IRQRRR+QYA++KE+C+QMFPVIGSGR+ 
Sbjct: 61  RGGIHPSIRGEVWEFLLGCYDPMSTFEEREQIRQRRRLQYASWKEECKQMFPVIGSGRFT 120

Query: 121 TAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTGATDANSDGPRSNPVNNLEPVQDQKII 180
           TAPVITE+GQP +DPLVL E N                NS+G             D+KII
Sbjct: 121 TAPVITENGQPNYDPLVLQEIN-------------LGTNSNGSVFFKELTSRGPLDKKII 180

Query: 181 QWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPMI 240
           QW+LTLHQIGLDV RTDR LVFYEK+ENLSKLWDIL+VYAWID DVGYCQGMSDLCSPMI
Sbjct: 181 QWLLTLHQIGLDVNRTDRALVFYEKKENLSKLWDILSVYAWIDNDVGYCQGMSDLCSPMI 240

Query: 241 MLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLSNLAAITQVIDPKLHQHLETLGG 300
           +LLEDE DAFWCFERLMRRLRGNFR T  SVGVE QL++L++ITQV+DPKLHQHL+ LGG
Sbjct: 241 ILLEDEADAFWCFERLMRRLRGNFRSTGRSVGVEAQLTHLSSITQVVDPKLHQHLDKLGG 300

Query: 301 GDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDMFAVYEETDMFSEKGEGSKGKA 360
           GDYLFA RMLMV FRREFSFCDSLYLWEMMWALEYDPD+F VYE     +EK EG KGK 
Sbjct: 301 GDYLFAIRMLMVQFRREFSFCDSLYLWEMMWALEYDPDLFYVYEAHQCGNEKTEGLKGKP 360

Query: 361 KSMRQCGKYERENLK--AKNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDM 420
           KS++QCGKYER+N++   K+++ PLPISVFLVASVLKDKS KL+TEARGLDDVVKILND 
Sbjct: 361 KSIKQCGKYERQNMRNGGKSAEGPLPISVFLVASVLKDKSYKLMTEARGLDDVVKILNDT 420

Query: 421 TGNLDAKKACTGAMKLHKKYLKK 442
           TGNLDAKK C+GA+K+HK+YL+K
Sbjct: 421 TGNLDAKKTCSGAIKIHKRYLRK 430

BLAST of Lag0018520 vs. TAIR 10
Match: AT2G20440.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 515.4 bits (1326), Expect = 4.6e-146
Identity = 252/444 (56.76%), Postives = 322/444 (72.52%), Query Frame = 0

Query: 1   MWRDAGAPADSFYEVRPEC-TDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRI 60
           MW+  G     FY VRPEC  DVP+TRFK RAGKTLS R+W AAF  +G LD+ K L RI
Sbjct: 7   MWKSGGEDLQGFYPVRPECQPDVPRTRFKSRAGKTLSARRWHAAFTEDGHLDMEKVLRRI 66

Query: 61  HRGGIHPSIRGEVWEFLLGCYDPTSTFEEREEIRQRRRIQYATFKEDCRQMFPVIGSGRY 120
            RGGIHPSI+G VWEFLLGCYDP STFEER  +R RRR QY  +KE+C++M PVIGSG+Y
Sbjct: 67  QRGGIHPSIKGAVWEFLLGCYDPDSTFEERNILRNRRREQYGAWKEECKKMVPVIGSGKY 126

Query: 121 ITAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTGATDANSDGPRSNPVNNLEPVQDQKI 180
           +T  V+ E+G PI       E++ +N   + ++T                    V D+++
Sbjct: 127 VTMAVVQENGNPID------ESSVENQGWIVKNT--------------------VTDERV 186

Query: 181 IQWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPM 240
           +QWML+LHQIGLDV RTDR L FYE   N SKLWD+LA+Y W++ D+GY QGM+D+CSPM
Sbjct: 187 LQWMLSLHQIGLDVARTDRYLCFYENDRNQSKLWDVLAIYTWLNLDIGYVQGMNDICSPM 246

Query: 241 IMLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLSNLAAITQVIDPKLHQHLETLG 300
           I+L +DEGDAFWCFER MRRLR NFR T +S+GV+TQL  L+ + + +DP+LHQHLE L 
Sbjct: 247 IILFDDEGDAFWCFERAMRRLRENFRATATSMGVQTQLGVLSQVIKTVDPRLHQHLEDLD 306

Query: 301 GGDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDMFAVYEETDMFSEKGEGSKGK 360
           GG+YLFA RMLMVLFRREFSF D+LYLWE+MWA+EY+P MFA YEE     E    +   
Sbjct: 307 GGEYLFAIRMLMVLFRREFSFLDALYLWELMWAMEYNPTMFATYEEL----ENRNNAASD 366

Query: 361 AKSMRQCGKYERENLKA-KNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDM 420
            K +++ GK+ER+ + + +N Q    ++VF+VASVL+ K+ +LL EA+GLDDVV+IL D+
Sbjct: 367 PKLLKRYGKFERKYINSGQNEQHRNTLAVFVVASVLQTKNKRLLKEAKGLDDVVQILGDI 420

Query: 421 TGNLDAKKACTGAMKLHKKYLKKA 443
            GNLDAKKAC  A+K+H+K+LKKA
Sbjct: 427 AGNLDAKKACKEALKIHEKFLKKA 420

BLAST of Lag0018520 vs. TAIR 10
Match: AT2G20440.2 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 515.4 bits (1326), Expect = 4.6e-146
Identity = 252/444 (56.76%), Postives = 322/444 (72.52%), Query Frame = 0

Query: 1   MWRDAGAPADSFYEVRPEC-TDVPKTRFKIRAGKTLSVRKWQAAFNPEGQLDISKTLNRI 60
           MW+  G     FY VRPEC  DVP+TRFK RAGKTLS R+W AAF  +G LD+ K L RI
Sbjct: 7   MWKSGGEDLQGFYPVRPECQPDVPRTRFKSRAGKTLSARRWHAAFTEDGHLDMEKVLRRI 66

Query: 61  HRGGIHPSIRGEVWEFLLGCYDPTSTFEEREEIRQRRRIQYATFKEDCRQMFPVIGSGRY 120
            RGGIHPSI+G VWEFLLGCYDP STFEER  +R RRR QY  +KE+C++M PVIGSG+Y
Sbjct: 67  QRGGIHPSIKGAVWEFLLGCYDPDSTFEERNILRNRRREQYGAWKEECKKMVPVIGSGKY 126

Query: 121 ITAPVITEDGQPIHDPLVLLETNPDNGPAVPQDTGATDANSDGPRSNPVNNLEPVQDQKI 180
           +T  V+ E+G PI       E++ +N   + ++T                    V D+++
Sbjct: 127 VTMAVVQENGNPID------ESSVENQGWIVKNT--------------------VTDERV 186

Query: 181 IQWMLTLHQIGLDVVRTDRTLVFYEKQENLSKLWDILAVYAWIDKDVGYCQGMSDLCSPM 240
           +QWML+LHQIGLDV RTDR L FYE   N SKLWD+LA+Y W++ D+GY QGM+D+CSPM
Sbjct: 187 LQWMLSLHQIGLDVARTDRYLCFYENDRNQSKLWDVLAIYTWLNLDIGYVQGMNDICSPM 246

Query: 241 IMLLEDEGDAFWCFERLMRRLRGNFRCTDSSVGVETQLSNLAAITQVIDPKLHQHLETLG 300
           I+L +DEGDAFWCFER MRRLR NFR T +S+GV+TQL  L+ + + +DP+LHQHLE L 
Sbjct: 247 IILFDDEGDAFWCFERAMRRLRENFRATATSMGVQTQLGVLSQVIKTVDPRLHQHLEDLD 306

Query: 301 GGDYLFAFRMLMVLFRREFSFCDSLYLWEMMWALEYDPDMFAVYEETDMFSEKGEGSKGK 360
           GG+YLFA RMLMVLFRREFSF D+LYLWE+MWA+EY+P MFA YEE     E    +   
Sbjct: 307 GGEYLFAIRMLMVLFRREFSFLDALYLWELMWAMEYNPTMFATYEEL----ENRNNAASD 366

Query: 361 AKSMRQCGKYERENLKA-KNSQAPLPISVFLVASVLKDKSTKLLTEARGLDDVVKILNDM 420
            K +++ GK+ER+ + + +N Q    ++VF+VASVL+ K+ +LL EA+GLDDVV+IL D+
Sbjct: 367 PKLLKRYGKFERKYINSGQNEQHRNTLAVFVVASVLQTKNKRLLKEAKGLDDVVQILGDI 420

Query: 421 TGNLDAKKACTGAMKLHKKYLKKA 443
            GNLDAKKAC  A+K+H+K+LKKA
Sbjct: 427 AGNLDAKKACKEALKIHEKFLKKA 420