Lag0018468 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0018468
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionVacuolar protein sorting-associated protein
Locationchr5: 27872268 .. 27876442 (-)
RNA-Seq ExpressionLag0018468
SyntenyLag0018468
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGAGAAGACCTGGCATTGGAGGCCTGCAGACAGCTGCGGCAGCGAGGGTTCTTTTCTGCTCAATCTATTTCTTTTCCTCCAAAATTTATGAATTTTAGCAATTTACTTGCATTACTATTCGGATTTCTATGGGTTTTATTGTTCATCTTCATTAATTTTGAAGTTCTTTCTTCTTTTGAGATTGTGATTTCTATTAATTAACCGGTGATAACAATATTTGTAGGATCAATACCGTCAGCTGGGTGAAAATGTGGCGAAGCTCAGAACTGACATGATGAAGGAGCAGCTCTCCACCTTTCGATCTCAGCTAGAGGATTTCGCTCGCAAACACAAGGTCTTTGTTCCTACTACAACACCTGCCCAGTATTTTGCTTTCAAATTTATAAGACCAATGAAGTTATCTGCTGGAATTTCTGCGTTGAGATATACTTATTTAATTAGAAGTTTTGTTTTGCAATATTGAACTGATTACGAATTTAAGACTGTTGCTTTATGTTTTCACTTGAATAAATACTTCCTTTGTTGATGTTGGAATTGGAAAGTAATGTACTACACTTTCGACTATGTAATGGCGGCTTATTCTTGCTTCCAAATTATTCCTGACCCAAAAAATAGAGAAGGCTGCGGGATTGCTTATTGTCTCGTTTTATGGTTGTTTCCGTTTTGGCCATTGGCAAAGTATTATCAGTTCATTCACCATAATAAAATTGAACTTCAAGTCGCTAAATTGATTTGTGCAGAAAGATATCAGCAAGAACCCGGGTTTCAGATCTCAGTTTCATGAAATGTGTGCTAAAGTTGGAGTAGATCCCTTGGCCTCAAACAAGGGGTTTTGGGCAGAGCTATTAGGCATTGGTGATTTCTATTACGAGATTGGTAGGTATGGATTTGATCTTACCATATGAAGTAAAAGAAAATTTTGTGGTTTATCGTGTCGAGAGGGATTGATGCGTCCCTCGCTCTCTTTCTAGGTGTGTTCTAATGAGTTATCTTTATCTTATATTCTTGCTCTATGTTTCTTCTCGAACTGCAGAAGATATTCCTTTTGCTTAAAAGAAAAATTGGCCTGAAGTTGAGGGCATGTGAAATTCTTATAGCATTTGCGTTCCTGTGCATGGTTATGTGATATGCCTGTGTTGACATGTTATGCATGTCCTCTTTGCCCGGCCAAGGAATTTTCATATGGATTTTTGGTTGTGTTTGGCCAAAACAGGGGAAATATCATAGTAATTTCTTTTTTTTTTTTAAATAGCAGCCTACTTATTCTTAGTTTGACTTAGAACTTGGAAGGGGAATTTCCCACTCTAGGAAGTATGAAGTTAACTGAGTGATATTTCAACTCAGTCATGAATTTTAAATTTCTCATGTGAAATTCCGCAAGTTTTTTCTTTGCTTATTAATTAAAGATATTGACAGTTGACAAGAATAAATGATTATTATGGTTGAAGCATAAGTCATTGTTCTAGTATTTCTCTATCTTCTTGCTGGATGTGTTTGATAAGAACTTTTTCTTTTTTTTTTTCTTTTTTTTTTTTTTTTTAAATGGGATAGCAAGCAATTTCATTGCTTAAATGAAATGATAAAAAGGGGATAAAATCATCCGAGATAATTACAAAAAGATTCTCCAGCTAGAGATAAGAGAGGGAGGAATTTTTTTGCAGCAAAATAGGGCCAAAAAGAGACTAGCTTCTGACATAATTTCTATATTTTTTTCCTTTTCTTGGAAGATTCTGGCTAAATGTAGTTAAACTATTTTGCTAGACAGGAGTGCAAATTGTTGACATTTGCTTGTCTACAAGACCCCACAATGGAGGTTTGATTAGCTTACAAGAACTCTGTACTTTACTTCGGCAGAGACGAAAAAGTGATCGTGAAGCTGTTTCTGAGGATGACTGCCTGCGAGCTATAAGTAAACTGAAGGTATTGAGATAATGGCGAAATCCATAATCAATACTCTCTCTCTCTCTCTCTCTCTCATAATATTTGGATGCTCATTGTGCTCAGGGGTTTCTTAAGAATCATTCCAAAACTGTGACTGTCTTATATTTGACATTGGTTGAAAATTGGTTGAAAGTATTTATTTATAAAATGGAACATATTTGTGTTTCCGGGTTTTAGTATTAGCTAATTCAGATATTGCTTGAATAGTCTTTGAATTTTGATTTTCATCAACCAATATGTTAACCATCTTGGCTGAGTCCAGAGGTCAAAGGAACAAGCTATAAATCCCAAATGTGTTTGAAACCATGAGTTCAAACAGTAGTCTTTACCCACCAAAGATATTATCTCTCAAATTTCCAGTCAGATCCGATATTATATATCTCGCAAGTTTCCTAGTGTCAGTGAGGAGCATAAACTAGCCTTTATTCTCACAGTCATCTTAATAGAAGTCATGTCTATGGGGTAAATTGATGAAAGTATCATTGTTATCAACAATATATTCATTAACTTATTTGCAAGCTCTAGTTATAGATATGATGATAGGTTGCGATGTAAGCCCTTCTGAAAATAATCGGCAATCTTTATGTAAGGTATCCAAACTAAATAACCTGCACAAGAGACAATAACACAAGAGACCAGCAAATAGATAACTCCCTACTCTGTTTCATTGAGAGTTCTTCAATTAACAGTAGTATTATAGGATAATATTGATAATAAGCAAACCAAACAGTCACAAGGGTTCCCCAGCTATTCGAGAGAGCTAGACTCTCCAACAAGACTCACAAACAAGCCAAAAGACAAAGACTCCTTAACTAATTACAACTGCCCTCACTACTAATCTAATACTATTCTCAATATATCCCTCACTAGTACTCTCACACTTTCTTATCAACAATCGTCATTAACATTACAGCGTCCACCAATATTTGTTGCCATGTCATAGATTTTCATGGATACCCATTGACATTTTTCCATATATCTGAGAATATCCATATTACTGTGTAAAAGAATCTGAATTTTAAGACAGATTATAAGTCATTTACATGTAACTCAGTTATTATATATATTATCTATTTCTTTCTTTGTATTTATCATTGTACCTCTTTCTTTAAAATCCGTAATATTTATATTTAAAGTATATAATAAGCAAGATACCCATTAGTCATTTACATTTATAGACATGAGTACTTAAAATGTTTCTTTTTTGTTTTCTTTTTGTTTTTTATTTTTTATTTTTTGTTTTTATTTTTTTTGTTCTTATTTTTATTTTTTTTGTTTTTTTACATTTTAAAAAATTATATATAAATTTCACTAAATTCAAATAATATCTACAAGTTCCTTGACTGCATATTTTTTTTCTTTAAATATTCATCATTATAGGAATAAATTCAATGCACCAAAACCAATGTTATGGATATTTTCATTTTTTGGTGGGATAAAGCTTTCTTCACAGTAAGGATGACTGTTAGATGAAAGTAGTTATCCTGGCAGGACAATTTTCTCCTCTAGGGAAGGTTGATTCCAACTAAAATCTTACCTTTATGTTATGTAGCTGGGGGAGATCGTAATAGACTAGTCTGTGGGCTTTTAGTAATACAAATATTTATATGCGTGACTATTGTTTTCCCTGGGGCACCTTTATTATCGTTTGTTACTTCTTAATGGAAAATATTTTACTTTGAATGGACTAAAGGTCTGATTTACGAAGTGGCATTCTGGAGCGTATTTTATACTAACATTCAGATAATTTACTCCAGATATTGGGAAGTGGTTTTGAGGTAATTACTGTTGGCAAGAAAAAACTTGTTCGGTCTGTTCCAACCGAGTTGAATAAAGATCACAATGAAATTCTTGAGCTTGCCCAGGTTCTTTACACATTCAGCCCTTTCTGCTAAGTATTTGCCTTTCGTATTTGGCCACAAAGAGAATAATGGTCATACCACAAACTGTTCTCTCAGGCTCAGGGCTATGTAACTGTCGATGAGGTAGAGAGACGACTTTCTTGGACGTCTGGTCGTGCAGTCGATGCCCTTGATACTCTCCTGGATGTAAGTTTTAGCTCACATTTCTCTTCTCCCATCTAGCATAATTTGACATTGCATTTGTAAAAGATTTTGCACTTTTACAGGAAGGTCTTGCGATGATTGATGATGGCCACCGTGACGGTAGACGTCGTTATTGGTTTCCTTGTGTTTCTCCTATTTCAGCTTTTCTCAGTACTGATGCCTGA

mRNA sequence

ATGAGGAGAAGACCTGGCATTGGAGGCCTGCAGACAGCTGCGGCAGCGAGGGATCAATACCGTCAGCTGGGTGAAAATGTGGCGAAGCTCAGAACTGACATGATGAAGGAGCAGCTCTCCACCTTTCGATCTCAGCTAGAGGATTTCGCTCGCAAACACAAGAAAGATATCAGCAAGAACCCGGGTTTCAGATCTCAGTTTCATGAAATGTGTGCTAAAGTTGGAGTAGATCCCTTGGCCTCAAACAAGGGGTTTTGGGCAGAGCTATTAGGCATTGGTGATTTCTATTACGAGATTGGAGTGCAAATTGTTGACATTTGCTTGTCTACAAGACCCCACAATGGAGGTTTGATTAGCTTACAAGAACTCTGTACTTTACTTCGGCAGAGACGAAAAAGTGATCGTGAAGCTGTTTCTGAGGATGACTGCCTGCGAGCTATAAGTAAACTGAAGATATTGGGAAGTGGTTTTGAGGTAATTACTGTTGGCAAGAAAAAACTTGTTCGGTCTGTTCCAACCGAGTTGAATAAAGATCACAATGAAATTCTTGAGCTTGCCCAGGCTCAGGGCTATGTAACTGTCGATGAGGTAGAGAGACGACTTTCTTGGACGTCTGGTCGTGCAGTCGATGCCCTTGATACTCTCCTGGATGAAGGTCTTGCGATGATTGATGATGGCCACCGTGACGGTAGACGTCGTTATTGGTTTCCTTGTGTTTCTCCTATTTCAGCTTTTCTCAGTACTGATGCCTGA

Coding sequence (CDS)

ATGAGGAGAAGACCTGGCATTGGAGGCCTGCAGACAGCTGCGGCAGCGAGGGATCAATACCGTCAGCTGGGTGAAAATGTGGCGAAGCTCAGAACTGACATGATGAAGGAGCAGCTCTCCACCTTTCGATCTCAGCTAGAGGATTTCGCTCGCAAACACAAGAAAGATATCAGCAAGAACCCGGGTTTCAGATCTCAGTTTCATGAAATGTGTGCTAAAGTTGGAGTAGATCCCTTGGCCTCAAACAAGGGGTTTTGGGCAGAGCTATTAGGCATTGGTGATTTCTATTACGAGATTGGAGTGCAAATTGTTGACATTTGCTTGTCTACAAGACCCCACAATGGAGGTTTGATTAGCTTACAAGAACTCTGTACTTTACTTCGGCAGAGACGAAAAAGTGATCGTGAAGCTGTTTCTGAGGATGACTGCCTGCGAGCTATAAGTAAACTGAAGATATTGGGAAGTGGTTTTGAGGTAATTACTGTTGGCAAGAAAAAACTTGTTCGGTCTGTTCCAACCGAGTTGAATAAAGATCACAATGAAATTCTTGAGCTTGCCCAGGCTCAGGGCTATGTAACTGTCGATGAGGTAGAGAGACGACTTTCTTGGACGTCTGGTCGTGCAGTCGATGCCCTTGATACTCTCCTGGATGAAGGTCTTGCGATGATTGATGATGGCCACCGTGACGGTAGACGTCGTTATTGGTTTCCTTGTGTTTCTCCTATTTCAGCTTTTCTCAGTACTGATGCCTGA

Protein sequence

MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKNPGFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISLQELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHNEILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVSPISAFLSTDA
Homology
BLAST of Lag0018468 vs. NCBI nr
Match: XP_008463432.1 (PREDICTED: vacuolar protein sorting-associated protein 22 homolog 1 [Cucumis melo] >KAA0033958.1 vacuolar protein sorting-associated protein 22-like protein 1 [Cucumis melo var. makuwa] >TYK02586.1 vacuolar protein sorting-associated protein 22-like protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 496.5 bits (1277), Expect = 1.3e-136
Identity = 246/250 (98.40%), Postives = 248/250 (99.20%), Query Frame = 0

Query: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60
           MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN
Sbjct: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60

Query: 61  PGFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120
           P FRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL
Sbjct: 61  PAFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120

Query: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180
           QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN
Sbjct: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180

Query: 181 EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240
           EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS
Sbjct: 181 EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240

Query: 241 PISAFLSTDA 251
           PIS+ L+TDA
Sbjct: 241 PISSSLTTDA 250

BLAST of Lag0018468 vs. NCBI nr
Match: XP_022943162.1 (vacuolar protein sorting-associated protein 22 homolog 1 [Cucurbita moschata] >XP_023515880.1 vacuolar protein sorting-associated protein 22 homolog 1 [Cucurbita pepo subsp. pepo] >KAG6600773.1 Vacuolar protein sorting-associated protein 22-like 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 495.4 bits (1274), Expect = 3.0e-136
Identity = 245/250 (98.00%), Postives = 247/250 (98.80%), Query Frame = 0

Query: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60
           MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN
Sbjct: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60

Query: 61  PGFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120
           P FRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL
Sbjct: 61  PAFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120

Query: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180
           QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN
Sbjct: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180

Query: 181 EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240
           EILELAQAQGYVTV+EVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS
Sbjct: 181 EILELAQAQGYVTVEEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240

Query: 241 PISAFLSTDA 251
           PIS+ L TDA
Sbjct: 241 PISSLLGTDA 250

BLAST of Lag0018468 vs. NCBI nr
Match: XP_022976277.1 (vacuolar protein sorting-associated protein 22 homolog 1 [Cucurbita maxima])

HSP 1 Score: 493.8 bits (1270), Expect = 8.7e-136
Identity = 244/250 (97.60%), Postives = 247/250 (98.80%), Query Frame = 0

Query: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60
           MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN
Sbjct: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60

Query: 61  PGFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120
           P FRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL
Sbjct: 61  PAFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120

Query: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180
           QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN
Sbjct: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180

Query: 181 EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240
           EILELAQAQGYVTV+EVER+LSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS
Sbjct: 181 EILELAQAQGYVTVEEVERQLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240

Query: 241 PISAFLSTDA 251
           PIS+ L TDA
Sbjct: 241 PISSLLGTDA 250

BLAST of Lag0018468 vs. NCBI nr
Match: XP_004152923.1 (vacuolar protein sorting-associated protein 22 homolog 1 [Cucumis sativus] >KGN56138.1 hypothetical protein Csa_011050 [Cucumis sativus])

HSP 1 Score: 493.0 bits (1268), Expect = 1.5e-135
Identity = 244/250 (97.60%), Postives = 246/250 (98.40%), Query Frame = 0

Query: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60
           MRRRPGIGGLQTAAAARDQYRQLGENVAKLR DMMKEQLSTFRSQLEDFARKHKKDISKN
Sbjct: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRADMMKEQLSTFRSQLEDFARKHKKDISKN 60

Query: 61  PGFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120
           P FRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL
Sbjct: 61  PAFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120

Query: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180
           QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN
Sbjct: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180

Query: 181 EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240
           EILELAQAQGY TVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS
Sbjct: 181 EILELAQAQGYATVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240

Query: 241 PISAFLSTDA 251
           PIS+ L+TDA
Sbjct: 241 PISSSLTTDA 250

BLAST of Lag0018468 vs. NCBI nr
Match: XP_038905901.1 (vacuolar protein sorting-associated protein 22 homolog 1 [Benincasa hispida])

HSP 1 Score: 493.0 bits (1268), Expect = 1.5e-135
Identity = 244/250 (97.60%), Postives = 246/250 (98.40%), Query Frame = 0

Query: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60
           MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDI KN
Sbjct: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDIGKN 60

Query: 61  PGFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120
           P FRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL
Sbjct: 61  PAFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120

Query: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180
           QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN
Sbjct: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180

Query: 181 EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240
           EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGL+MIDDGHRDGRRRYWFPCVS
Sbjct: 181 EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLSMIDDGHRDGRRRYWFPCVS 240

Query: 241 PISAFLSTDA 251
           PIS+ L TDA
Sbjct: 241 PISSSLPTDA 250

BLAST of Lag0018468 vs. ExPASy Swiss-Prot
Match: Q5M759 (Vacuolar protein sorting-associated protein 22 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VP22-1 PE=2 SV=2)

HSP 1 Score: 433.3 bits (1113), Expect = 1.8e-120
Identity = 205/249 (82.33%), Postives = 230/249 (92.37%), Query Frame = 0

Query: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60
           MRRRPGIGGLQ AAAARDQYR LGENVAKLRTDMMKEQLSTFRSQLE+FARKHK DI KN
Sbjct: 1   MRRRPGIGGLQKAAAARDQYRLLGENVAKLRTDMMKEQLSTFRSQLEEFARKHKNDIRKN 60

Query: 61  PGFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120
           P FR+QFHEMCA +GVDPLASNKGFWAELLGIGDFYYE+GVQI+++C+ TR HNGGLISL
Sbjct: 61  PAFRAQFHEMCANIGVDPLASNKGFWAELLGIGDFYYELGVQIIEVCMLTRSHNGGLISL 120

Query: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180
           QELC  LRQRRK DREAV+EDDCLRAISKLK+LGSGFEVIT+GKKKLVRSVPTELNKDHN
Sbjct: 121 QELCNHLRQRRKKDREAVTEDDCLRAISKLKVLGSGFEVITIGKKKLVRSVPTELNKDHN 180

Query: 181 EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240
           +ILELAQ QG+V V+EV+RRLSWTSGR +DAL+TLL+EGLAMID+GH+DG+ RYWFPCVS
Sbjct: 181 QILELAQGQGFVIVEEVQRRLSWTSGRVIDALETLLEEGLAMIDNGHKDGKCRYWFPCVS 240

Query: 241 PISAFLSTD 250
            + + + +D
Sbjct: 241 SVYSSIGSD 249

BLAST of Lag0018468 vs. ExPASy Swiss-Prot
Match: Q5RJU0 (Vacuolar-sorting protein SNF8 OS=Xenopus tropicalis OX=8364 GN=snf8 PE=2 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.0e-62
Identity = 118/238 (49.58%), Postives = 165/238 (69.33%), Query Frame = 0

Query: 2   RRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKNP 61
           RR  G G +     A  +Y++ G  +A+ +   M +QL  F++ LE+FA KHK++I KNP
Sbjct: 3   RRGVGAGAIAKKKLAEAKYKERGTVLAEDQLLQMSKQLEMFKTNLEEFASKHKQEIRKNP 62

Query: 62  GFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISLQ 121
            FR QF +MCA +GVDPLAS KGFW+E+LG+GDFYYE+GVQI+++CL+ + HNGGLI+L 
Sbjct: 63  QFRVQFQDMCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHHNGGLITLA 122

Query: 122 ELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHNE 181
           EL   + + R    + VS+DD +RAI KLK+LG+GF +I VG   LV+SVP ELN DH  
Sbjct: 123 ELHQHVLKGRGKLAQDVSQDDIIRAIKKLKVLGNGFGIIPVGGSYLVQSVPAELNMDHTV 182

Query: 182 ILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCV 240
           +L+LA+ +G+VTV+E++  L+W + RA   L+ LL EGLA I D    G  +YW P +
Sbjct: 183 VLQLAEKKGFVTVNEIKSSLNWETERAKHVLEHLLKEGLAWI-DSQAQGEAQYWLPAL 239

BLAST of Lag0018468 vs. ExPASy Swiss-Prot
Match: Q5U3V9 (Vacuolar-sorting protein SNF8 OS=Danio rerio OX=7955 GN=snf8 PE=2 SV=2)

HSP 1 Score: 237.7 bits (605), Expect = 1.5e-61
Identity = 121/248 (48.79%), Postives = 163/248 (65.73%), Query Frame = 0

Query: 2   RRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKNP 61
           RR  G G +     A  +Y++ G  +A+ +   M +QL TF++ LE+FA KHK++I K+ 
Sbjct: 3   RRGVGAGAIAKKKLAEAKYKERGSVLAEDQIAQMSKQLDTFKTHLEEFASKHKQEIRKSS 62

Query: 62  GFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISLQ 121
            FR QF EMCA +GVDPLAS KGFW+E+LG+GDFYYE+GVQI+++CL+ +  NGGLI+L 
Sbjct: 63  QFRVQFQEMCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHRNGGLITLD 122

Query: 122 ELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHNE 181
           EL   + + R    + VS+DD +RAI KLK +G+GF +I VG   LV+SVP ELN DH  
Sbjct: 123 ELHHRVLKGRGKFAQDVSQDDLVRAIKKLKAMGNGFGMIPVGGTYLVQSVPAELNMDHTV 182

Query: 182 ILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVSP 241
           +L+LA+ +GYVTV E+   L W   RA   LD LL EGLA + D    G  +YW P +  
Sbjct: 183 VLQLAEKKGYVTVSEIRESLKWEKERACHVLDHLLKEGLAWL-DSQAAGEPQYWLPAL-- 242

Query: 242 ISAFLSTD 250
            S  LS D
Sbjct: 243 FSELLSQD 247

BLAST of Lag0018468 vs. ExPASy Swiss-Prot
Match: Q96H20 (Vacuolar-sorting protein SNF8 OS=Homo sapiens OX=9606 GN=SNF8 PE=1 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 4.3e-61
Identity = 116/238 (48.74%), Postives = 160/238 (67.23%), Query Frame = 0

Query: 2   RRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKNP 61
           RR  G G +     A  +Y++ G  +A+ +   M +QL  F++ LE+FA KHK++I KNP
Sbjct: 3   RRGVGAGAIAKKKLAEAKYKERGTVLAEDQLAQMSKQLDMFKTNLEEFASKHKQEIRKNP 62

Query: 62  GFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISLQ 121
            FR QF +MCA +GVDPLAS KGFW+E+LG+GDFYYE+GVQI+++CL+ +  NGGLI+L+
Sbjct: 63  EFRVQFQDMCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHRNGGLITLE 122

Query: 122 ELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHNE 181
           EL   + + R    + VS+DD +RAI KLK LG+GF +I VG   L++SVP ELN DH  
Sbjct: 123 ELHQQVLKGRGKFAQDVSQDDLIRAIKKLKALGTGFGIIPVGGTYLIQSVPAELNMDHTV 182

Query: 182 ILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCV 240
           +L+LA+  GYVTV E++  L W + RA   L+ LL EGLA + D    G   YW P +
Sbjct: 183 VLQLAEKNGYVTVSEIKASLKWETERARQVLEHLLKEGLAWL-DLQAPGEAHYWLPAL 239

BLAST of Lag0018468 vs. ExPASy Swiss-Prot
Match: Q9CZ28 (Vacuolar-sorting protein SNF8 OS=Mus musculus OX=10090 GN=Snf8 PE=1 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 4.3e-61
Identity = 116/238 (48.74%), Postives = 160/238 (67.23%), Query Frame = 0

Query: 2   RRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKNP 61
           RR  G G +     A  +Y++ G  +A+ +   M +QL  F++ LE+FA KHK++I KNP
Sbjct: 3   RRGVGAGAIAKKKLAEAKYKERGTVLAEDQLAQMSKQLDMFKTNLEEFASKHKQEIRKNP 62

Query: 62  GFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISLQ 121
            FR QF +MCA +GVDPLAS KGFW+E+LG+GDFYYE+GVQI+++CL+ +  NGGLI+L+
Sbjct: 63  EFRVQFQDMCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHRNGGLITLE 122

Query: 122 ELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHNE 181
           EL   + + R    + VS+DD +RAI KLK LG+GF +I VG   L++SVP ELN DH  
Sbjct: 123 ELHQQVLKGRGKFAQDVSQDDLIRAIKKLKALGTGFGIIPVGGTYLIQSVPAELNMDHTV 182

Query: 182 ILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCV 240
           +L+LA+  GYVTV E++  L W + RA   L+ LL EGLA + D    G   YW P +
Sbjct: 183 VLQLAEKNGYVTVSEIKTSLKWETERARQVLEHLLKEGLAWL-DLQAPGEAHYWLPAL 239

BLAST of Lag0018468 vs. ExPASy TrEMBL
Match: A0A5A7STK1 (Vacuolar protein sorting-associated protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold925G00390 PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 6.5e-137
Identity = 246/250 (98.40%), Postives = 248/250 (99.20%), Query Frame = 0

Query: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60
           MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN
Sbjct: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60

Query: 61  PGFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120
           P FRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL
Sbjct: 61  PAFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120

Query: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180
           QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN
Sbjct: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180

Query: 181 EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240
           EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS
Sbjct: 181 EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240

Query: 241 PISAFLSTDA 251
           PIS+ L+TDA
Sbjct: 241 PISSSLTTDA 250

BLAST of Lag0018468 vs. ExPASy TrEMBL
Match: A0A1S3CKT6 (Vacuolar protein sorting-associated protein OS=Cucumis melo OX=3656 GN=LOC103501596 PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 6.5e-137
Identity = 246/250 (98.40%), Postives = 248/250 (99.20%), Query Frame = 0

Query: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60
           MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN
Sbjct: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60

Query: 61  PGFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120
           P FRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL
Sbjct: 61  PAFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120

Query: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180
           QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN
Sbjct: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180

Query: 181 EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240
           EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS
Sbjct: 181 EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240

Query: 241 PISAFLSTDA 251
           PIS+ L+TDA
Sbjct: 241 PISSSLTTDA 250

BLAST of Lag0018468 vs. ExPASy TrEMBL
Match: A0A6J1FS90 (Vacuolar protein sorting-associated protein OS=Cucurbita moschata OX=3662 GN=LOC111447975 PE=3 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 1.4e-136
Identity = 245/250 (98.00%), Postives = 247/250 (98.80%), Query Frame = 0

Query: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60
           MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN
Sbjct: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60

Query: 61  PGFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120
           P FRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL
Sbjct: 61  PAFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120

Query: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180
           QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN
Sbjct: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180

Query: 181 EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240
           EILELAQAQGYVTV+EVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS
Sbjct: 181 EILELAQAQGYVTVEEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240

Query: 241 PISAFLSTDA 251
           PIS+ L TDA
Sbjct: 241 PISSLLGTDA 250

BLAST of Lag0018468 vs. ExPASy TrEMBL
Match: A0A6J1IFB3 (Vacuolar protein sorting-associated protein OS=Cucurbita maxima OX=3661 GN=LOC111476717 PE=3 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 4.2e-136
Identity = 244/250 (97.60%), Postives = 247/250 (98.80%), Query Frame = 0

Query: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60
           MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN
Sbjct: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60

Query: 61  PGFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120
           P FRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL
Sbjct: 61  PAFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120

Query: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180
           QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN
Sbjct: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180

Query: 181 EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240
           EILELAQAQGYVTV+EVER+LSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS
Sbjct: 181 EILELAQAQGYVTVEEVERQLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240

Query: 241 PISAFLSTDA 251
           PIS+ L TDA
Sbjct: 241 PISSLLGTDA 250

BLAST of Lag0018468 vs. ExPASy TrEMBL
Match: A0A0A0L307 (Vacuolar protein sorting-associated protein OS=Cucumis sativus OX=3659 GN=Csa_3G077720 PE=3 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 7.2e-136
Identity = 244/250 (97.60%), Postives = 246/250 (98.40%), Query Frame = 0

Query: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60
           MRRRPGIGGLQTAAAARDQYRQLGENVAKLR DMMKEQLSTFRSQLEDFARKHKKDISKN
Sbjct: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRADMMKEQLSTFRSQLEDFARKHKKDISKN 60

Query: 61  PGFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120
           P FRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL
Sbjct: 61  PAFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120

Query: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180
           QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN
Sbjct: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180

Query: 181 EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240
           EILELAQAQGY TVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS
Sbjct: 181 EILELAQAQGYATVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240

Query: 241 PISAFLSTDA 251
           PIS+ L+TDA
Sbjct: 241 PISSSLTTDA 250

BLAST of Lag0018468 vs. TAIR 10
Match: AT4G27040.1 (EAP30/Vps36 family protein )

HSP 1 Score: 433.3 bits (1113), Expect = 1.3e-121
Identity = 205/249 (82.33%), Postives = 230/249 (92.37%), Query Frame = 0

Query: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60
           MRRRPGIGGLQ AAAARDQYR LGENVAKLRTDMMKEQLSTFRSQLE+FARKHK DI KN
Sbjct: 1   MRRRPGIGGLQKAAAARDQYRLLGENVAKLRTDMMKEQLSTFRSQLEEFARKHKNDIRKN 60

Query: 61  PGFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120
           P FR+QFHEMCA +GVDPLASNKGFWAELLGIGDFYYE+GVQI+++C+ TR HNGGLISL
Sbjct: 61  PAFRAQFHEMCANIGVDPLASNKGFWAELLGIGDFYYELGVQIIEVCMLTRSHNGGLISL 120

Query: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180
           QELC  LRQRRK DREAV+EDDCLRAISKLK+LGSGFEVIT+GKKKLVRSVPTELNKDHN
Sbjct: 121 QELCNHLRQRRKKDREAVTEDDCLRAISKLKVLGSGFEVITIGKKKLVRSVPTELNKDHN 180

Query: 181 EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240
           +ILELAQ QG+V V+EV+RRLSWTSGR +DAL+TLL+EGLAMID+GH+DG+ RYWFPCVS
Sbjct: 181 QILELAQGQGFVIVEEVQRRLSWTSGRVIDALETLLEEGLAMIDNGHKDGKCRYWFPCVS 240

Query: 241 PISAFLSTD 250
            + + + +D
Sbjct: 241 SVYSSIGSD 249

BLAST of Lag0018468 vs. TAIR 10
Match: AT4G27040.2 (EAP30/Vps36 family protein )

HSP 1 Score: 433.3 bits (1113), Expect = 1.3e-121
Identity = 205/249 (82.33%), Postives = 230/249 (92.37%), Query Frame = 0

Query: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60
           MRRRPGIGGLQ AAAARDQYR LGENVAKLRTDMMKEQLSTFRSQLE+FARKHK DI KN
Sbjct: 1   MRRRPGIGGLQKAAAARDQYRLLGENVAKLRTDMMKEQLSTFRSQLEEFARKHKNDIRKN 60

Query: 61  PGFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120
           P FR+QFHEMCA +GVDPLASNKGFWAELLGIGDFYYE+GVQI+++C+ TR HNGGLISL
Sbjct: 61  PAFRAQFHEMCANIGVDPLASNKGFWAELLGIGDFYYELGVQIIEVCMLTRSHNGGLISL 120

Query: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180
           QELC  LRQRRK DREAV+EDDCLRAISKLK+LGSGFEVIT+GKKKLVRSVPTELNKDHN
Sbjct: 121 QELCNHLRQRRKKDREAVTEDDCLRAISKLKVLGSGFEVITIGKKKLVRSVPTELNKDHN 180

Query: 181 EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240
           +ILELAQ QG+V V+EV+RRLSWTSGR +DAL+TLL+EGLAMID+GH+DG+ RYWFPCVS
Sbjct: 181 QILELAQGQGFVIVEEVQRRLSWTSGRVIDALETLLEEGLAMIDNGHKDGKCRYWFPCVS 240

Query: 241 PISAFLSTD 250
            + + + +D
Sbjct: 241 SVYSSIGSD 249

BLAST of Lag0018468 vs. TAIR 10
Match: AT4G27040.3 (EAP30/Vps36 family protein )

HSP 1 Score: 433.3 bits (1113), Expect = 1.3e-121
Identity = 205/249 (82.33%), Postives = 230/249 (92.37%), Query Frame = 0

Query: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60
           MRRRPGIGGLQ AAAARDQYR LGENVAKLRTDMMKEQLSTFRSQLE+FARKHK DI KN
Sbjct: 1   MRRRPGIGGLQKAAAARDQYRLLGENVAKLRTDMMKEQLSTFRSQLEEFARKHKNDIRKN 60

Query: 61  PGFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120
           P FR+QFHEMCA +GVDPLASNKGFWAELLGIGDFYYE+GVQI+++C+ TR HNGGLISL
Sbjct: 61  PAFRAQFHEMCANIGVDPLASNKGFWAELLGIGDFYYELGVQIIEVCMLTRSHNGGLISL 120

Query: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180
           QELC  LRQRRK DREAV+EDDCLRAISKLK+LGSGFEVIT+GKKKLVRSVPTELNKDHN
Sbjct: 121 QELCNHLRQRRKKDREAVTEDDCLRAISKLKVLGSGFEVITIGKKKLVRSVPTELNKDHN 180

Query: 181 EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240
           +ILELAQ QG+V V+EV+RRLSWTSGR +DAL+TLL+EGLAMID+GH+DG+ RYWFPCVS
Sbjct: 181 QILELAQGQGFVIVEEVQRRLSWTSGRVIDALETLLEEGLAMIDNGHKDGKCRYWFPCVS 240

Query: 241 PISAFLSTD 250
            + + + +D
Sbjct: 241 SVYSSIGSD 249

BLAST of Lag0018468 vs. TAIR 10
Match: AT4G27040.4 (EAP30/Vps36 family protein )

HSP 1 Score: 433.3 bits (1113), Expect = 1.3e-121
Identity = 205/249 (82.33%), Postives = 230/249 (92.37%), Query Frame = 0

Query: 1   MRRRPGIGGLQTAAAARDQYRQLGENVAKLRTDMMKEQLSTFRSQLEDFARKHKKDISKN 60
           MRRRPGIGGLQ AAAARDQYR LGENVAKLRTDMMKEQLSTFRSQLE+FARKHK DI KN
Sbjct: 1   MRRRPGIGGLQKAAAARDQYRLLGENVAKLRTDMMKEQLSTFRSQLEEFARKHKNDIRKN 60

Query: 61  PGFRSQFHEMCAKVGVDPLASNKGFWAELLGIGDFYYEIGVQIVDICLSTRPHNGGLISL 120
           P FR+QFHEMCA +GVDPLASNKGFWAELLGIGDFYYE+GVQI+++C+ TR HNGGLISL
Sbjct: 61  PAFRAQFHEMCANIGVDPLASNKGFWAELLGIGDFYYELGVQIIEVCMLTRSHNGGLISL 120

Query: 121 QELCTLLRQRRKSDREAVSEDDCLRAISKLKILGSGFEVITVGKKKLVRSVPTELNKDHN 180
           QELC  LRQRRK DREAV+EDDCLRAISKLK+LGSGFEVIT+GKKKLVRSVPTELNKDHN
Sbjct: 121 QELCNHLRQRRKKDREAVTEDDCLRAISKLKVLGSGFEVITIGKKKLVRSVPTELNKDHN 180

Query: 181 EILELAQAQGYVTVDEVERRLSWTSGRAVDALDTLLDEGLAMIDDGHRDGRRRYWFPCVS 240
           +ILELAQ QG+V V+EV+RRLSWTSGR +DAL+TLL+EGLAMID+GH+DG+ RYWFPCVS
Sbjct: 181 QILELAQGQGFVIVEEVQRRLSWTSGRVIDALETLLEEGLAMIDNGHKDGKCRYWFPCVS 240

Query: 241 PISAFLSTD 250
            + + + +D
Sbjct: 241 SVYSSIGSD 249

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008463432.11.3e-13698.40PREDICTED: vacuolar protein sorting-associated protein 22 homolog 1 [Cucumis mel... [more]
XP_022943162.13.0e-13698.00vacuolar protein sorting-associated protein 22 homolog 1 [Cucurbita moschata] >X... [more]
XP_022976277.18.7e-13697.60vacuolar protein sorting-associated protein 22 homolog 1 [Cucurbita maxima][more]
XP_004152923.11.5e-13597.60vacuolar protein sorting-associated protein 22 homolog 1 [Cucumis sativus] >KGN5... [more]
XP_038905901.11.5e-13597.60vacuolar protein sorting-associated protein 22 homolog 1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q5M7591.8e-12082.33Vacuolar protein sorting-associated protein 22 homolog 1 OS=Arabidopsis thaliana... [more]
Q5RJU01.0e-6249.58Vacuolar-sorting protein SNF8 OS=Xenopus tropicalis OX=8364 GN=snf8 PE=2 SV=1[more]
Q5U3V91.5e-6148.79Vacuolar-sorting protein SNF8 OS=Danio rerio OX=7955 GN=snf8 PE=2 SV=2[more]
Q96H204.3e-6148.74Vacuolar-sorting protein SNF8 OS=Homo sapiens OX=9606 GN=SNF8 PE=1 SV=1[more]
Q9CZ284.3e-6148.74Vacuolar-sorting protein SNF8 OS=Mus musculus OX=10090 GN=Snf8 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7STK16.5e-13798.40Vacuolar protein sorting-associated protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3CKT66.5e-13798.40Vacuolar protein sorting-associated protein OS=Cucumis melo OX=3656 GN=LOC103501... [more]
A0A6J1FS901.4e-13698.00Vacuolar protein sorting-associated protein OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1IFB34.2e-13697.60Vacuolar protein sorting-associated protein OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A0A0L3077.2e-13697.60Vacuolar protein sorting-associated protein OS=Cucumis sativus OX=3659 GN=Csa_3G... [more]
Match NameE-valueIdentityDescription
AT4G27040.11.3e-12182.33EAP30/Vps36 family protein [more]
AT4G27040.21.3e-12182.33EAP30/Vps36 family protein [more]
AT4G27040.31.3e-12182.33EAP30/Vps36 family protein [more]
AT4G27040.41.3e-12182.33EAP30/Vps36 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 13..33
NoneNo IPR availableGENE3D6.10.140.180coord: 24..85
e-value: 3.4E-26
score: 92.7
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 91..171
e-value: 9.2E-36
score: 123.6
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 172..247
e-value: 1.1E-26
score: 94.5
IPR040608Snf8/Vps36 familyPFAMPF04157EAP30coord: 6..225
e-value: 1.3E-76
score: 257.0
IPR016689ESCRT-2 complex, Snf8PIRSFPIRSF017215ESCRT2_Vps22coord: 1..244
e-value: 1.2E-94
score: 313.2
IPR016689ESCRT-2 complex, Snf8PANTHERPTHR12806EAP30 SUBUNIT OF ELL COMPLEXcoord: 1..240
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 24..174
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 175..238

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0018468.1Lag0018468.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
biological_process GO:0071985 multivesicular body sorting pathway
cellular_component GO:0000814 ESCRT II complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0008168 methyltransferase activity