Lag0018427 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0018427
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
Location	chr5: 26830407 .. 26832978 (-)
RNA-Seq Expression	Lag0018427
Synteny	Lag0018427

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGAAGCTTTTCGGGAAAATTGCCGGCTTCTTCAGCAATAGAACTCTGGTGGGAGTGGACAAAGCGGGAAATAGATACTTCTTTGTAAAAGAAGAAATCGATGGCATAAGTAAGTAGGTCTAATTCCCTTTTTAAATTGCTCATTCTCATTGTTCCCTAATTCCATTTGATCGGAATGAATTTCCAGATTCCACCTGAGTTTCTACTTTCTACTTCTTTGCAATTGGATTATAACATTTTAATCATTTGGACCTTTTCCCTCTATCTCACTCGACATTATATGGATGATATTCAAGACTCAAGAGATATGGAGAGATAGAGCGCTCATGATTTAGCGTGATTGATATATAGCTGCCTTTGTTACTCATACAATGAAACTTTTATCACTGTACATATACGGCTCACACAACTTTCACTCATTGGTTTGCTAGTGAAAGAGAAGAGATGGGTGGTGTTTAAGGGTGAGGATGATCCAACGTCAATTCCCGGTAAGCATCTATGGACCTATGGTTGAGACTTAAGAGAATGGCTTTGTTATTTGAATCATCTGTTTATGTTATTTTTAAATCAGGGTTTAAAATATAAGGCCAATTTTGGGAACTTGAATGATGTAAGACAAGCTGGATATGTAGTTTAGGAGGAACTCAACCTGCTGATAGTAGGTTTTTCTGATGATGATTACTTATATTTTTATTGCTGCAGTGGAATGGATATGTTGGTTAAATGGGCAGCGTAAAAAGGCTCCAACCCCAGAGGTAACTCAATACTTCTTCCATGATCAATTGAATCTCTTTGCATCCCCCTTGATCGGTCAGAAGATTGGTGATTGGTGTGCTACTCATGGTTTAATATTTCAATTGTTCTTTTGTCATTTCCTTCAGGAAATGATGGAGCTAGAAGCTAGGCGAGAACGTGTTCGACAAAATGTTGCCCGTATGTTGACTCTTCAATAAAATTTTGCTTCTTATTCTTTGGTCGTTCATGGCAAAATATTTTACATTATGTATTTAAAACTGACAACTTCTCTTTATCAAAATTGTGACTCATATACAGTCAGAAAGGGATGAATTGTTTTGTACCTGAAATTGCCAAATATAAGAATCACCCAACATATTCATATTTGAGAACTCCTTTTCGTAGGAACCAATGCTGGACTTCACAATCTTAACTATGACATTTCTTACATATTCTTTTTATCTTCCTTAGTAGATCTTCTTATTAGATGGTTTAGGCTTCCAGGAGGCATTCATCTAGCTCCACCCTTTTTTGTAGACTGACCTAGGAAAACTAGGTTGTTAGTAGTATAGGACTAATGGTTTTACTAGGTTGTCAGTAGTGTAGGACCAATGGTCTTACTTGTTCTCCTCTCAATCACCATGTATGTTGGTTTAATGATATTATTATACTGCCTGTCTGCTTGAATTGTCTGCTTTGCATGGTATTTGCTTTGCCTGGATTGGTTACATCATCTAGTATGAAATTTTGTTGCTTGGATTATTTGCCTTGTTATTGTATGATTATTGTTTGACTTGCTTTGCCTGCTTATTGATTGACTTGTTTGTCTTGTATGAACATGCTCGACCCATCACATTCCAACATACTTTAATTGAACTCAAAAAGGTCCAAAATATGTTGAGCTCACTAACCTTACCCAAAAAGGATTTGAAACTAATTAAAAGGAATTTAGTTTCCTTTTCAAACTAGGAAAATTAATTCTAACTCAAGGGACTAGATTGCAAGATTTTAATTTTCCAAGTTAGAATAAAAACGTCCAATTAGTCCAAAATAGCCCGATATCGAAATGCGGGATGTTACACGAGTAACATTTGATCAGCAGTTCAAAATTGAATTTTACTTGATACTTTTAGAAGAACCAATATCATTCTCATTCTTCGACTTAACAAGGTCTTATTGGAAGTTATCTTTGTTTAATTTAGTTCTCAAGAAAGAAGAGATGGAGAGAAATTCTAAAGAAGGCAATTTAAGGAAAGTCATCAGCACTGGTCAGTTCCTTACTGACAAACTCAAGTGCTTGTTACACTTGCAGCATATTGCTAACATTGTTTTACTGCACAGGTCAAGTTGGAGGTCCTGACTTGAAAAGCTTTATTCGACAATTTCCAACTGGTTCAGAAGGTATCATATGCTTTAATAATATATTTTCTTACCCCTTTATTTCCCTCCCCGTTCCCAACCCAAGGAAAGGGAAATGTCTTTGCCCCGATCCTCAAATAGGATTTTCTTAAGGGCTATGGAGATTGAAAGCCGGACTTCCCGTTTACTTGCAAAAAGCATTTGATTTAAAATAATCATTTAGAATGAGCTAATTTTGGTTGATTACTTATCAAAAAAGAATGAGCTAATTTTTTCTCCTTTTGGTTGATTACTTTCTTGTACTTCTTCTGGTCACATTTACCTCTCTTTCTCTCCCAAATTGATAGTTGTTCTTTTCATTTGAATCAGAGTATATTTTATTGATTGTATGCTTATAACTGTTTATTCATTTTGGTATTTCTTGCATAAAGATACCCAAGATGAGGAAGGATTGGCTGCAAGGGGGATATCTTGA

mRNA sequence

ATGTCGAAGCTTTTCGGGAAAATTGCCGGCTTCTTCAGCAATAGAACTCTGGTGGGAGTGGACAAAGCGGGAAATAGATACTTCTTTGTAAAAGAAGAAATCGATGGCATAATGAAAGAGAAGAGATGGGTGGTGTTTAAGGGTGAGGATGATCCAACGTCAATTCCCGTGGAATGGATATGTTGGTTAAATGGGCAGCGTAAAAAGGCTCCAACCCCAGAGGAAATGATGGAGCTAGAAGCTAGGCGAGAACGTGTTCGACAAAATGTTGCCCTTCTCAAGAAAGAAGAGATGGAGAGAAATTCTAAAGAAGGCAATTTAAGGAAAGTCATCAGCACTGGTCAAGTTGGAGGTCCTGACTTGAAAAGCTTTATTCGACAATTTCCAACTGGTTCAGAAGATACCCAAGATGAGGAAGGATTGGCTGCAAGGGGGATATCTTGA

Coding sequence (CDS)

ATGTCGAAGCTTTTCGGGAAAATTGCCGGCTTCTTCAGCAATAGAACTCTGGTGGGAGTGGACAAAGCGGGAAATAGATACTTCTTTGTAAAAGAAGAAATCGATGGCATAATGAAAGAGAAGAGATGGGTGGTGTTTAAGGGTGAGGATGATCCAACGTCAATTCCCGTGGAATGGATATGTTGGTTAAATGGGCAGCGTAAAAAGGCTCCAACCCCAGAGGAAATGATGGAGCTAGAAGCTAGGCGAGAACGTGTTCGACAAAATGTTGCCCTTCTCAAGAAAGAAGAGATGGAGAGAAATTCTAAAGAAGGCAATTTAAGGAAAGTCATCAGCACTGGTCAAGTTGGAGGTCCTGACTTGAAAAGCTTTATTCGACAATTTCCAACTGGTTCAGAAGATACCCAAGATGAGGAAGGATTGGCTGCAAGGGGGATATCTTGA

Protein sequence

MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWICWLNGQRKKAPTPEEMMELEARRERVRQNVALLKKEEMERNSKEGNLRKVISTGQVGGPDLKSFIRQFPTGSEDTQDEEGLAARGIS

Homology

[Close]

BLAST of Lag0018427 vs. NCBI nr
Match: XP_038903173.1 (uncharacterized protein LOC120089839 isoform X1 [Benincasa hispida])

HSP 1 Score: 276.6 bits (706), Expect = 1.3e-70
Identity = 137/144 (95.14%), Postives = 138/144 (95.83%), Query Frame = 0


Query: 1   MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWI 60
           MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWI
Sbjct: 1   MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWI 60

Query: 61  CWLNGQRKKAPTPEEMMELEARRERVRQNVALLKKEEMERNSKEGNLRKVISTGQVGGPD 120
           CWLNGQRKKAPTPEEMMELEARRERVRQNVALLKKEEMERNSKEGNLRK+ISTGQV GPD
Sbjct: 61  CWLNGQRKKAPTPEEMMELEARRERVRQNVALLKKEEMERNSKEGNLRKIISTGQVAGPD 120

Query: 121 LKSFIRQFPTGSEDTQDEEGLAAR 145
           LKSFIRQFPTGSE  QD EGL  R
Sbjct: 121 LKSFIRQFPTGSEGAQDVEGLTTR 144

[Close]

BLAST of Lag0018427 vs. NCBI nr
Match: XP_038903174.1 (uncharacterized protein LOC120089839 isoform X2 [Benincasa hispida])

HSP 1 Score: 276.6 bits (706), Expect = 1.3e-70
Identity = 137/144 (95.14%), Postives = 138/144 (95.83%), Query Frame = 0


Query: 1   MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWI 60
           MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWI
Sbjct: 1   MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWI 60

Query: 61  CWLNGQRKKAPTPEEMMELEARRERVRQNVALLKKEEMERNSKEGNLRKVISTGQVGGPD 120
           CWLNGQRKKAPTPEEMMELEARRERVRQNVALLKKEEMERNSKEGNLRK+ISTGQV GPD
Sbjct: 61  CWLNGQRKKAPTPEEMMELEARRERVRQNVALLKKEEMERNSKEGNLRKIISTGQVAGPD 120

Query: 121 LKSFIRQFPTGSEDTQDEEGLAAR 145
           LKSFIRQFPTGSE  QD EGL  R
Sbjct: 121 LKSFIRQFPTGSEGAQDVEGLTTR 144

[Close]

BLAST of Lag0018427 vs. NCBI nr
Match: KAA0033972.1 (NDUFA12 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 270.0 bits (689), Expect = 1.2e-68
Identity = 135/144 (93.75%), Postives = 136/144 (94.44%), Query Frame = 0


Query: 1   MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWI 60
           MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWI
Sbjct: 1   MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWI 60

Query: 61  CWLNGQRKKAPTPEEMMELEARRERVRQNVALLKKEEMERNSKEGNLRKVISTGQVGGPD 120
           CWLNGQRKKAPTPEEMMELEARRERVRQNVALLKKEEMERNSKEG LRK+ISTGQV GPD
Sbjct: 61  CWLNGQRKKAPTPEEMMELEARRERVRQNVALLKKEEMERNSKEGKLRKIISTGQVMGPD 120

Query: 121 LKSFIRQFPTGSEDTQDEEGLAAR 145
           LKSFIRQ PTGSE  QD EGL  R
Sbjct: 121 LKSFIRQVPTGSEGAQDVEGLPTR 144

[Close]

BLAST of Lag0018427 vs. NCBI nr
Match: XP_004152928.1 (uncharacterized protein LOC101214441 isoform X2 [Cucumis sativus] >KGN56129.1 hypothetical protein Csa_010369 [Cucumis sativus])

HSP 1 Score: 266.5 bits (680), Expect = 1.3e-67
Identity = 133/144 (92.36%), Postives = 135/144 (93.75%), Query Frame = 0


Query: 1   MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWI 60
           MSKLFGKIA FFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWV+FKGEDDPTSIPVEWI
Sbjct: 1   MSKLFGKIAAFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVLFKGEDDPTSIPVEWI 60

Query: 61  CWLNGQRKKAPTPEEMMELEARRERVRQNVALLKKEEMERNSKEGNLRKVISTGQVGGPD 120
           CWLNGQRKKAPTPEEM ELEARRERVRQNVALLKKEEMERNSKEGNLRK+ISTGQV GPD
Sbjct: 61  CWLNGQRKKAPTPEEMRELEARRERVRQNVALLKKEEMERNSKEGNLRKIISTGQVMGPD 120

Query: 121 LKSFIRQFPTGSEDTQDEEGLAAR 145
           LKSFIRQ PTGSE  QD EGL  R
Sbjct: 121 LKSFIRQVPTGSEGAQDVEGLPTR 144

[Close]

BLAST of Lag0018427 vs. NCBI nr
Match: XP_031738721.1 (uncharacterized protein LOC101214441 isoform X1 [Cucumis sativus])

HSP 1 Score: 266.5 bits (680), Expect = 1.3e-67
Identity = 133/144 (92.36%), Postives = 135/144 (93.75%), Query Frame = 0


Query: 1   MSKLFGKIAGFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKGEDDPTSIPVEWI 60
           MSKLFGKIA FFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWV+FKGEDDPTSIPVEWI
Sbjct: 1   MSKLFGKIAAFFSNRTLVGVDKAGNRYFFVKEEIDGIMKEKRWVLFKGEDDPTSIPVEWI 60

Query: 61  CWLNGQRKKAPTPEEMMELEARRERVRQNVALLKKEEMERNSKEGNLRKVISTGQVGGPD 120
           CWLNGQRKKAPTPEEM ELEARRERVRQNVALLKKEEMERNSKEGNLRK+ISTGQV GPD
Sbjct: 61  CWLNGQRKKAPTPEEMRELEARRERVRQNVALLKKEEMERNSKEGNLRKIISTGQVMGPD 120

Query: 121 LKSFIRQFPTGSEDTQDEEGLAAR 145
           LKSFIRQ PTGSE  QD EGL  R
Sbjct: 121 LKSFIRQVPTGSEGAQDVEGLPTR 144

[Close]

BLAST of Lag0018427 vs. ExPASy Swiss-Prot
Match: Q54MV7 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 OS=Dictyostelium discoideum OX=44689 GN=ndufa12 PE=2 SV=2)

HSP 1 Score: 48.9 bits (115), Expect = 5.7e-05
Identity = 26/59 (44.07%), Postives = 36/59 (61.02%), Query Frame = 0


Query: 16 TLVGVDKAGNRYFFVKEEIDGIMKEKRWVVFKG--EDDPTSIPVEWICWLNGQRKKAPT 73
          TLVGVDK GNRY+  ++EI G     RWV +     +DPT+IP E+  W++    K P+
Sbjct: 33 TLVGVDKVGNRYYENRQEIYG---RHRWVEYGDYKSNDPTTIPPEYHSWIHHVSDKLPS 88

[Close]

BLAST of Lag0018427 vs. ExPASy Swiss-Prot
Match: Q59J78 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2 OS=Mus musculus OX=10090 GN=Ndufaf2 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 4.8e-04
Identity = 35/124 (28.23%), Postives = 60/124 (48.39%), Query Frame = 0


Query: 15  RTLVGVDKAGNRYFFVKEEID---GIMKEKRWVVFKG----EDDPTSIPVEWICWLNGQR 74
           R  VG D  GN+Y++V E  +     ++EKR V        + +   IP EW  W+   R
Sbjct: 20  REHVGTDHLGNKYYYVAEYKNWRGQTIREKRIVEAANRKEVDYEAGDIPTEWEAWIRRTR 79

Query: 75  KKAPTPEEMMELEARRERVR-QNVALLKKEEMERNSKEGNLRKVISTGQVGGPDLKSFIR 131
           K  PT EE+++ E  RE ++ ++    +K+++ + + E  L    +T   G      F R
Sbjct: 80  KTPPTMEEILKNEKYREEIKIKSQDFYEKDKLGKETSEELLPSPTATQVKGHASAPYFGR 139

The following BLAST results are available for this feature:

BLAST of Lag0018427 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903173.1	1.3e-70	95.14	uncharacterized protein LOC120089839 isoform X1 [Benincasa hispida]	[more]
XP_038903174.1	1.3e-70	95.14	uncharacterized protein LOC120089839 isoform X2 [Benincasa hispida]	[more]
KAA0033972.1	1.2e-68	93.75	NDUFA12 domain-containing protein [Cucumis melo var. makuwa]	[more]
XP_004152928.1	1.3e-67	92.36	uncharacterized protein LOC101214441 isoform X2 [Cucumis sativus] >KGN56129.1 hy...	[more]
XP_031738721.1	1.3e-67	92.36	uncharacterized protein LOC101214441 isoform X1 [Cucumis sativus]	[more]

BLAST of Lag0018427 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54MV7	5.7e-05	44.07	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 OS=Dictyostelium d...	[more]
Q59J78	4.8e-04	28.23	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2 OS=Mus musc...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 76..96
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 99..147
None	No IPR available	PANTHER	PTHR12910:SF1	NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 12	coord: 1..136
IPR007763	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12	PFAM	PF05071	NDUFA12	coord: 16..96 e-value: 8.8E-13 score: 49.0
IPR007763	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12	PANTHER	PTHR12910	NADH-UBIQUINONE OXIDOREDUCTASE SUBUNIT B17.2	coord: 1..136

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0018427.1	Lag0018427.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032981	mitochondrial respiratory chain complex I assembly
cellular_component	GO:0005743	mitochondrial inner membrane
cellular_component	GO:0070469	respirasome
cellular_component	GO:0016020	membrane