Lag0018185 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0018185
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase
Locationchr5: 18267594 .. 18269194 (+)
RNA-Seq ExpressionLag0018185
SyntenyLag0018185
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGTCAAACAAGCAGTTGACGAAGACCCATGTGGAACATTTAGTTGAGATCGAAGAGCAGTTGCTCTACTTGAGGGAAGTCCCCGATGCTGTTCGGTTTGTGGAATCTCGACTGGAGGAGATTTCGATCAAGGCCGACAAAGTTGATGCGGTAGCTGGCCGCGTGGATGGAATGCCCATCCAAAAACTGATGATGAGGGTTGAGAACCTTGAAAGTAAAGCTACTCGACCTAGTAGCTACGAGCGTGGTGACAGCTCGTCGGGCTCTGCTGCTCTGATGGAGGAGCGCGTCGATAGCTTAGACAGCTCCCAGAAAGTTATAGTCCAGATGGTCACGGACTTGTCAGAAGATGTGAAAGCTGCCCTTGACGTGGTCAGGGCCGAAGTGGCGGATCTGAGCGCCAGATTGAACCTCACGATGAGAGTGAGAAAGAACCTACCTCCGAGGAGAGGAATTGACCACGAGATTGAGTTGTTGCCTGGGGCAAAGCCGCCCGCCAAGAATGCCTACCGTATGGCACCTCCCGAGTTAGCAGAACTTCGGAAACAACTCGACGAGTTACTGAATGCGGGATTTATCCGCCCAGCAAAGGCTCCTTATGGGGCCCCAGTCTTGTTCCAAAAGAAGTAGAAGAAATTTTGGCAGACAGAGTTCGTCGCACAGGTAAACCTCGTCGAGACGTTCAAGAGTTCTTGGTGAAGTGGAAAGGGCTCCCGGATGCTGAGATTAGTTGGGAGCGAGTTGAAGATCTGAAGTCGGCTGCTATCAAGATCGCAGAGTTCGAGCAGCATCGGTTGACAGGGACGTCAACCATTTAAGTGGGGGAGGATGTCACGGTCATGCTCGTCCAAGGCGTGTTGCCCATGGCCGCATGCCCATGACAACCCTTTCCCCTCATGCTTTCATTATATGCATTCCCTTTGTATCGCTTGTTTATTTTTCCTTTCTTTTCTCTAGTGTATGACTCCTCGTGATTTTCATGTTCAAGCCTGCGAAGGCTAAAAATCTCAATAATATACTATAGGGGGGTAAGACTAGTCGTCAATCCTTCCTACCCGGAGTTGTTTGCTATCAAGCTGTTGTTGTCCCTTTGTTTACTTGATAGTCTGCAACACATATACATGCTTTCCAAAATGCTTTCAAACGTTCTCCAAAACCTTTTCTCTAACCCCGTTTTCTAAAACCGTTTTCCAAGAACGGGGGTAGAGGCTGCGATATTCGCCCGTTGTGCCACTGGCGATCCGTTTTTGAGTTGGCTGACTTACTTGTGAGCGAAAGTAAGTGCCGCACAGTCGCGTTACGAGACCCTAAAGACCGCGATTGTGACACTCTGCACTAGGCCTGACATATGTTATGCAGTGGGAGTAGTCGGTAGGTATCAGTCTAATCCAGGGTTGGACCATTGGGGTGCAGTTAAGAATATCCTCAAGTATCTTCGGAGAATGAGGGACTACATGCTTGTGTATGGAGCTAAGGATTTGATTCTTACTGGATACATTGACTCTGACTTTCAAGCTGATAAGGATTCAAGGAAATCCACATCAGGGTCAATATTCACCCTCAATGGAGGAGCAGTAGTTTGGAGAAGCATCAAATAA

mRNA sequence

ATGTCGTCAAACAAGCAGTTGACGAAGACCCATGTGGAACATTTAGTTGAGATCGAAGAGCAGTTGCTCTACTTGAGGGAAGTCCCCGATGCTGTTCGGTTTGTGGAATCTCGACTGGAGGAGATTTCGATCAAGGCCGACAAAGTTGATGCGGTAGCTGGCCGCGTGGATGGAATGCCCATCCAAAAACTGATGATGAGGGTTGAGAACCTTGAAAGTAAAGCTACTCGACCTAGTAGCTACGAGCGTGGTGACAGCTCGTCGGGCTCTGCTGCTCTGATGGAGGAGCGCGTCGATAGCTTAGACAGCTCCCAGAAAGTTATAGTCCAGATGGTCACGGACTTGTCAGAAGATGTGAAAGCTGCCCTTGACGTGGTCAGGGCCGAAGTGGCGGATCTGAGCGCCAGATTGAACCTCACGATGAGAGTGAGAAAGAACCTACCTCCGAGGAGAGGAATTGACCACGAGATTGAGTTGTTGCCTGGGGCAAAGCCGCCCGCCAAGAATGCCTACCGTATGGCACCTCCCGAGTTAGCAGAACTTCGGAAACAACTCGACGAGTTACTGAATGCGGGATTTATCCGCCCAGCAAAGGCTCCTTATGGGGCCCCAGTCTTGTTCCAAAAGAAATCTGAAGTCGGCTGCTATCAAGATCGCAGAGTTCGAGCAGCATCGGTTGACAGGGACGTCAACCATTTAAGTGGGGGAGGATGTCACGGTCATGCTCGTCCAAGGCGTGTTGCCCATGGCCGCATGCCCATGACAACCCTTTCCCCTCATGCTTTCATTATATGCATTCCCTTTGTATCGCTTAACGGGGGTAGAGGCTGCGATATTCGCCCGTTGTGCCACTGGCGATCCGTTTTTGAGTTGGCTGACTTACTTGTGAGCGAAATGGGAGTAGTCGGTAGGTATCAGTCTAATCCAGGGTTGGACCATTGGGGTGCAGTTAAGAATATCCTCAAGTATCTTCGGAGAATGAGGGACTACATGCTTGTGTATGGAGCTAAGGATTTGATTCTTACTGGATACATTGACTCTGACTTTCAAGCTGATAAGGATTCAAGGAAATCCACATCAGGGTCAATATTCACCCTCAATGGAGGAGCAGTAGTTTGGAGAAGCATCAAATAA

Coding sequence (CDS)

ATGTCGTCAAACAAGCAGTTGACGAAGACCCATGTGGAACATTTAGTTGAGATCGAAGAGCAGTTGCTCTACTTGAGGGAAGTCCCCGATGCTGTTCGGTTTGTGGAATCTCGACTGGAGGAGATTTCGATCAAGGCCGACAAAGTTGATGCGGTAGCTGGCCGCGTGGATGGAATGCCCATCCAAAAACTGATGATGAGGGTTGAGAACCTTGAAAGTAAAGCTACTCGACCTAGTAGCTACGAGCGTGGTGACAGCTCGTCGGGCTCTGCTGCTCTGATGGAGGAGCGCGTCGATAGCTTAGACAGCTCCCAGAAAGTTATAGTCCAGATGGTCACGGACTTGTCAGAAGATGTGAAAGCTGCCCTTGACGTGGTCAGGGCCGAAGTGGCGGATCTGAGCGCCAGATTGAACCTCACGATGAGAGTGAGAAAGAACCTACCTCCGAGGAGAGGAATTGACCACGAGATTGAGTTGTTGCCTGGGGCAAAGCCGCCCGCCAAGAATGCCTACCGTATGGCACCTCCCGAGTTAGCAGAACTTCGGAAACAACTCGACGAGTTACTGAATGCGGGATTTATCCGCCCAGCAAAGGCTCCTTATGGGGCCCCAGTCTTGTTCCAAAAGAAATCTGAAGTCGGCTGCTATCAAGATCGCAGAGTTCGAGCAGCATCGGTTGACAGGGACGTCAACCATTTAAGTGGGGGAGGATGTCACGGTCATGCTCGTCCAAGGCGTGTTGCCCATGGCCGCATGCCCATGACAACCCTTTCCCCTCATGCTTTCATTATATGCATTCCCTTTGTATCGCTTAACGGGGGTAGAGGCTGCGATATTCGCCCGTTGTGCCACTGGCGATCCGTTTTTGAGTTGGCTGACTTACTTGTGAGCGAAATGGGAGTAGTCGGTAGGTATCAGTCTAATCCAGGGTTGGACCATTGGGGTGCAGTTAAGAATATCCTCAAGTATCTTCGGAGAATGAGGGACTACATGCTTGTGTATGGAGCTAAGGATTTGATTCTTACTGGATACATTGACTCTGACTTTCAAGCTGATAAGGATTCAAGGAAATCCACATCAGGGTCAATATTCACCCTCAATGGAGGAGCAGTAGTTTGGAGAAGCATCAAATAA

Protein sequence

MSSNKQLTKTHVEHLVEIEEQLLYLREVPDAVRFVESRLEEISIKADKVDAVAGRVDGMPIQKLMMRVENLESKATRPSSYERGDSSSGSAALMEERVDSLDSSQKVIVQMVTDLSEDVKAALDVVRAEVADLSARLNLTMRVRKNLPPRRGIDHEIELLPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPYGAPVLFQKKSEVGCYQDRRVRAASVDRDVNHLSGGGCHGHARPRRVAHGRMPMTTLSPHAFIICIPFVSLNGGRGCDIRPLCHWRSVFELADLLVSEMGVVGRYQSNPGLDHWGAVKNILKYLRRMRDYMLVYGAKDLILTGYIDSDFQADKDSRKSTSGSIFTLNGGAVVWRSIK
Homology
BLAST of Lag0018185 vs. NCBI nr
Match: TYK03099.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 160.6 bits (405), Expect = 2.6e-35
Identity = 89/154 (57.79%), Postives = 116/154 (75.32%), Query Frame = 0

Query: 1   MSSNKQLTKTHVEHLVEIEEQLLYLREVPDAVRFVESRLEEISIKADKVDAVAGRVDGMP 60
           MSS+  L K   + LVE+EEQ+LYL EVPD++R++ESRLEEIS K + +DAVAGRV+G P
Sbjct: 1   MSSSNPLGKAQKDRLVELEEQMLYLVEVPDSIRYLESRLEEISEKTNTIDAVAGRVEGFP 60

Query: 61  IQKLMMRVENLESKAT--RPSSYERGDSSSGSAALMEERVDSLDSSQKVIVQMVTDLSED 120
           IQ+LM RV+ LE+     R  +YERGDSS+GS A +EERV  LDSSQK +++M+  +SED
Sbjct: 61  IQELMTRVDALETTVNIGRTVNYERGDSSTGSVAHIEERVQELDSSQKTLLEMINGMSED 120

Query: 121 VKAALDVVRAEVADLSARLNLTMRVRKNLPPRRG 153
            +A LDVVR E+AD++ARL+LTMR   N  P  G
Sbjct: 121 FRATLDVVRNEIADVNARLSLTMRAMANQAPAGG 154

BLAST of Lag0018185 vs. NCBI nr
Match: TYK03099.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 131.3 bits (329), Expect = 1.7e-26
Identity = 62/68 (91.18%), Postives = 66/68 (97.06%), Query Frame = 0

Query: 145 KNLPPRRGIDHEIELLPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPYGAP 204
           K+LPPRR IDHEIEL+PGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPYGAP
Sbjct: 646 KSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPYGAP 705

Query: 205 VLFQKKSE 213
           VLFQ+K +
Sbjct: 706 VLFQRKKD 713


HSP 2 Score: 160.6 bits (405), Expect = 2.6e-35
Identity = 89/154 (57.79%), Postives = 119/154 (77.27%), Query Frame = 0

Query: 1   MSSNKQLTKTHVEHLVEIEEQLLYLREVPDAVRFVESRLEEISIKADKVDAVAGRVDGMP 60
           MSS+    K   + LVEIEEQ+LYL EVPD++R++ESR+EEIS KA+ +DAVAGRV+G+P
Sbjct: 1   MSSSNPSGKAQRDRLVEIEEQMLYLVEVPDSIRYLESRVEEISEKANMIDAVAGRVEGLP 60

Query: 61  IQKLMMRVENLE--SKATRPSSYERGDSSSGSAALMEERVDSLDSSQKVIVQMVTDLSED 120
           IQ+L+ RV+ LE  + A R  +YERG+SSSG AA MEERVD LD++QK +++M+  +SED
Sbjct: 61  IQELLARVDALEENTNARRTINYERGESSSGFAAHMEERVDELDNAQKTLLEMINGMSED 120

Query: 121 VKAALDVVRAEVADLSARLNLTMRVRKNLPPRRG 153
            +A LDVVR E+AD++ARL+LTMR   N  P  G
Sbjct: 121 FRATLDVVRNEIADVNARLSLTMRAMANQAPAGG 154

BLAST of Lag0018185 vs. NCBI nr
Match: KAA0067252.1 (uncharacterized protein E6C27_scaffold418G00690 [Cucumis melo var. makuwa] >TYK10861.1 uncharacterized protein E5676_scaffold231G00330 [Cucumis melo var. makuwa])

HSP 1 Score: 159.5 bits (402), Expect = 5.8e-35
Identity = 89/154 (57.79%), Postives = 115/154 (74.68%), Query Frame = 0

Query: 1   MSSNKQLTKTHVEHLVEIEEQLLYLREVPDAVRFVESRLEEISIKADKVDAVAGRVDGMP 60
           M S+    K   + LVEIEEQ+LYL EVPD++R++ESRL+EIS K D +DAVAGRV+G+P
Sbjct: 1   MLSSNPSGKAQKDRLVEIEEQMLYLVEVPDSIRYLESRLDEISEKTDMIDAVAGRVEGLP 60

Query: 61  IQKLMMRVENLE--SKATRPSSYERGDSSSGSAALMEERVDSLDSSQKVIVQMVTDLSED 120
           IQ+L+ RV+ LE  + A R  +YERGDSSSG AA M+ERV  LDSSQK +++M+  +SED
Sbjct: 61  IQELLARVDALEENTNARRTVNYERGDSSSGFAAHMKERVSELDSSQKTLLEMINGMSED 120

Query: 121 VKAALDVVRAEVADLSARLNLTMRVRKNLPPRRG 153
            +  LDVVR E+AD++ARLNLTMR   N  P  G
Sbjct: 121 FRVTLDVVRNEIADVNARLNLTMRAMANQAPAGG 154

BLAST of Lag0018185 vs. NCBI nr
Match: KAA0046343.1 (uncharacterized protein E6C27_scaffold149G00520 [Cucumis melo var. makuwa] >TYK30507.1 uncharacterized protein E5676_scaffold426G00500 [Cucumis melo var. makuwa])

HSP 1 Score: 159.1 bits (401), Expect = 7.6e-35
Identity = 85/152 (55.92%), Postives = 112/152 (73.68%), Query Frame = 0

Query: 1   MSSNKQLTKTHVEHLVEIEEQLLYLREVPDAVRFVESRLEEISIKADKVDAVAGRVDGMP 60
           MSS     K   + LVE+EEQ+LYL EVPD++RF+ESRLEEI+ K D +D VAGRV+G+P
Sbjct: 1   MSSANPSDKAQRDRLVELEEQMLYLAEVPDSIRFLESRLEEITEKTDTIDVVAGRVEGLP 60

Query: 61  IQKLMMRVENLESKATRPSSYERGDSSSGSAALMEERVDSLDSSQKVIVQMVTDLSEDVK 120
           IQ+L+ RV  LE    R  SYE GDSSSG  A MEER++ LDSSQK +++++ ++SED +
Sbjct: 61  IQELLARVNTLEGNIRRTVSYEYGDSSSGFVAHMEERINELDSSQKTLLEIINNMSEDFR 120

Query: 121 AALDVVRAEVADLSARLNLTMRVRKNLPPRRG 153
           A LDVVR E+AD++ARLN+T+R   N  P  G
Sbjct: 121 ATLDVVRNEIADVNARLNITIRAIANQAPAGG 152

BLAST of Lag0018185 vs. NCBI nr
Match: KAA0065409.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 158.7 bits (400), Expect = 1.0e-34
Identity = 92/168 (54.76%), Postives = 120/168 (71.43%), Query Frame = 0

Query: 1   MSSNKQLTKTHVEHLVEIEEQLLYLREVPDAVRFVESRLEEISIKADKVDAVAGRVDGMP 60
           MSS+    K   + LVE+EEQ+LYL EVPD++R++ESRLEEIS K + +DAVAGRV+G P
Sbjct: 1   MSSSNPSGKAQKDRLVELEEQMLYLVEVPDSIRYLESRLEEISEKTNTIDAVAGRVEGFP 60

Query: 61  IQKLMMRVENLESKAT--RPSSYERGDSSSGSAALMEERVDSLDSSQKVIVQMVTDLSED 120
           IQ+LM RV+ LE+     R  +YERGDSS+GS A +EERV  LDSSQK +++M+  +SED
Sbjct: 61  IQELMTRVDALETTVNIGRTVNYERGDSSTGSVAHIEERVQELDSSQKTLLEMINGMSED 120

Query: 121 VKAALDVVRAEVADLSARLNLTMRVRKN-LPPRRGIDHEIELLPGAKP 166
            +A LDVVR E+AD++ARL+LTMR   N  PP   I      +P  KP
Sbjct: 121 FRATLDVVRNEIADVNARLSLTMRAMANQAPPGGAIPVSRVKIPEPKP 168

BLAST of Lag0018185 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.4e-15
Identity = 39/77 (50.65%), Postives = 52/77 (67.53%), Query Frame = 0

Query: 299  MGVVGRYQSNPGLDHWGAVKNILKYLRRMRDYMLVYGAKDLILTGYIDSDFQADKDSRKS 358
            +GVV R+  NPG +HW AVK IL+YLR      L +G  D IL GY D+D   D D+RKS
Sbjct: 1133 VGVVSRFLENPGKEHWEAVKWILRYLRGTTGDCLCFGGSDPILKGYTDADMAGDIDNRKS 1192

Query: 359  TSGSIFTLNGGAVVWRS 376
            ++G +FT +GGA+ W+S
Sbjct: 1193 STGYLFTFSGGAISWQS 1209

BLAST of Lag0018185 vs. ExPASy Swiss-Prot
Match: P0CV72 (Secreted RxLR effector protein 161 OS=Plasmopara viticola OX=143451 GN=RXLR161 PE=2 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 2.5e-12
Identity = 37/84 (44.05%), Postives = 54/84 (64.29%), Query Frame = 0

Query: 295 LVSEMGVVGRYQSNPGLDHWGAVKNILKYLRRMRDYMLVY-GAKDLILTGYIDSDFQADK 354
           L + +GV+ ++ S+P   HW A+K +L+YL+  + Y L +  A    L GY D+D+  D 
Sbjct: 24  LAAAVGVLSQFASDPCPTHWQALKRVLRYLQSTQTYGLEFTRAGTAKLVGYSDADWAGDV 83

Query: 355 DSRKSTSGSIFTLNGGAVVWRSIK 378
           +SR+STSG +F LNGG V WRS K
Sbjct: 84  ESRRSTSGYLFKLNGGCVSWRSKK 107

BLAST of Lag0018185 vs. ExPASy Swiss-Prot
Match: Q99315 (Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-G PE=1 SV=3)

HSP 1 Score: 48.9 bits (115), Expect = 1.5e-04
Identity = 25/76 (32.89%), Postives = 41/76 (53.95%), Query Frame = 0

Query: 143 VRKNLPPRRG------IDHEIELLPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRP 202
           +R +LPPR        + H+IE+ PGA+ P    Y +      E+ K + +LL+  FI P
Sbjct: 568 IRNDLPPRPADINNIPVKHDIEIKPGARLPRLQPYHVTEKNEQEINKIVQKLLDNKFIVP 627

Query: 203 AKAPYGAPVLFQKKSE 213
           +K+P  +PV+   K +
Sbjct: 628 SKSPCSSPVVLVPKKD 643

BLAST of Lag0018185 vs. ExPASy Swiss-Prot
Match: Q7LHG5 (Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-I PE=1 SV=2)

HSP 1 Score: 48.9 bits (115), Expect = 1.5e-04
Identity = 25/76 (32.89%), Postives = 41/76 (53.95%), Query Frame = 0

Query: 143 VRKNLPPRRG------IDHEIELLPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRP 202
           +R +LPPR        + H+IE+ PGA+ P    Y +      E+ K + +LL+  FI P
Sbjct: 594 IRNDLPPRPADINNIPVKHDIEIKPGARLPRLQPYHVTEKNEQEINKIVQKLLDNKFIVP 653

Query: 203 AKAPYGAPVLFQKKSE 213
           +K+P  +PV+   K +
Sbjct: 654 SKSPCSSPVVLVPKKD 669

BLAST of Lag0018185 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 2.5e-04
Identity = 26/77 (33.77%), Postives = 38/77 (49.35%), Query Frame = 0

Query: 302  VGRYQSNPGLDHWGAVKNILKYLRRMRDY-MLVYGAKDLILTGYIDSDFQADKDSRKSTS 361
            + +Y   P  DHW A+K +L+YL    D+ + +     L L  Y D+D+  D D   ST+
Sbjct: 1252 LSQYMHMPTDDHWNALKRVLRYLAGTPDHGIFLKKGNTLSLHAYSDADWAGDTDDYVSTN 1311

Query: 362  GSIFTLNGGAVVWRSIK 378
            G I  L    + W S K
Sbjct: 1312 GYIVYLGHHPISWSSKK 1328

BLAST of Lag0018185 vs. ExPASy TrEMBL
Match: A0A5D3DHI2 (Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G00340 PE=4 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 1.3e-35
Identity = 89/154 (57.79%), Postives = 119/154 (77.27%), Query Frame = 0

Query: 1   MSSNKQLTKTHVEHLVEIEEQLLYLREVPDAVRFVESRLEEISIKADKVDAVAGRVDGMP 60
           MSS+    K   + LVEIEEQ+LYL EVPD++R++ESR+EEIS KA+ +DAVAGRV+G+P
Sbjct: 1   MSSSNPSGKAQRDRLVEIEEQMLYLVEVPDSIRYLESRVEEISEKANMIDAVAGRVEGLP 60

Query: 61  IQKLMMRVENLE--SKATRPSSYERGDSSSGSAALMEERVDSLDSSQKVIVQMVTDLSED 120
           IQ+L+ RV+ LE  + A R  +YERG+SSSG AA MEERVD LD++QK +++M+  +SED
Sbjct: 61  IQELLARVDALEENTNARRTINYERGESSSGFAAHMEERVDELDNAQKTLLEMINGMSED 120

Query: 121 VKAALDVVRAEVADLSARLNLTMRVRKNLPPRRG 153
            +A LDVVR E+AD++ARL+LTMR   N  P  G
Sbjct: 121 FRATLDVVRNEIADVNARLSLTMRAMANQAPAGG 154

BLAST of Lag0018185 vs. ExPASy TrEMBL
Match: A0A5D3BYE6 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00260 PE=4 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 1.3e-35
Identity = 89/154 (57.79%), Postives = 116/154 (75.32%), Query Frame = 0

Query: 1   MSSNKQLTKTHVEHLVEIEEQLLYLREVPDAVRFVESRLEEISIKADKVDAVAGRVDGMP 60
           MSS+  L K   + LVE+EEQ+LYL EVPD++R++ESRLEEIS K + +DAVAGRV+G P
Sbjct: 1   MSSSNPLGKAQKDRLVELEEQMLYLVEVPDSIRYLESRLEEISEKTNTIDAVAGRVEGFP 60

Query: 61  IQKLMMRVENLESKAT--RPSSYERGDSSSGSAALMEERVDSLDSSQKVIVQMVTDLSED 120
           IQ+LM RV+ LE+     R  +YERGDSS+GS A +EERV  LDSSQK +++M+  +SED
Sbjct: 61  IQELMTRVDALETTVNIGRTVNYERGDSSTGSVAHIEERVQELDSSQKTLLEMINGMSED 120

Query: 121 VKAALDVVRAEVADLSARLNLTMRVRKNLPPRRG 153
            +A LDVVR E+AD++ARL+LTMR   N  P  G
Sbjct: 121 FRATLDVVRNEIADVNARLSLTMRAMANQAPAGG 154

BLAST of Lag0018185 vs. ExPASy TrEMBL
Match: A0A5D3BYE6 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00260 PE=4 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 8.2e-27
Identity = 62/68 (91.18%), Postives = 66/68 (97.06%), Query Frame = 0

Query: 145 KNLPPRRGIDHEIELLPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPYGAP 204
           K+LPPRR IDHEIEL+PGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPYGAP
Sbjct: 646 KSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPYGAP 705

Query: 205 VLFQKKSE 213
           VLFQ+K +
Sbjct: 706 VLFQRKKD 713


HSP 2 Score: 159.5 bits (402), Expect = 2.8e-35
Identity = 89/154 (57.79%), Postives = 115/154 (74.68%), Query Frame = 0

Query: 1   MSSNKQLTKTHVEHLVEIEEQLLYLREVPDAVRFVESRLEEISIKADKVDAVAGRVDGMP 60
           M S+    K   + LVEIEEQ+LYL EVPD++R++ESRL+EIS K D +DAVAGRV+G+P
Sbjct: 1   MLSSNPSGKAQKDRLVEIEEQMLYLVEVPDSIRYLESRLDEISEKTDMIDAVAGRVEGLP 60

Query: 61  IQKLMMRVENLE--SKATRPSSYERGDSSSGSAALMEERVDSLDSSQKVIVQMVTDLSED 120
           IQ+L+ RV+ LE  + A R  +YERGDSSSG AA M+ERV  LDSSQK +++M+  +SED
Sbjct: 61  IQELLARVDALEENTNARRTVNYERGDSSSGFAAHMKERVSELDSSQKTLLEMINGMSED 120

Query: 121 VKAALDVVRAEVADLSARLNLTMRVRKNLPPRRG 153
            +  LDVVR E+AD++ARLNLTMR   N  P  G
Sbjct: 121 FRVTLDVVRNEIADVNARLNLTMRAMANQAPAGG 154

BLAST of Lag0018185 vs. ExPASy TrEMBL
Match: A0A5D3E355 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G00500 PE=4 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 3.7e-35
Identity = 85/152 (55.92%), Postives = 112/152 (73.68%), Query Frame = 0

Query: 1   MSSNKQLTKTHVEHLVEIEEQLLYLREVPDAVRFVESRLEEISIKADKVDAVAGRVDGMP 60
           MSS     K   + LVE+EEQ+LYL EVPD++RF+ESRLEEI+ K D +D VAGRV+G+P
Sbjct: 1   MSSANPSDKAQRDRLVELEEQMLYLAEVPDSIRFLESRLEEITEKTDTIDVVAGRVEGLP 60

Query: 61  IQKLMMRVENLESKATRPSSYERGDSSSGSAALMEERVDSLDSSQKVIVQMVTDLSEDVK 120
           IQ+L+ RV  LE    R  SYE GDSSSG  A MEER++ LDSSQK +++++ ++SED +
Sbjct: 61  IQELLARVNTLEGNIRRTVSYEYGDSSSGFVAHMEERINELDSSQKTLLEIINNMSEDFR 120

Query: 121 AALDVVRAEVADLSARLNLTMRVRKNLPPRRG 153
           A LDVVR E+AD++ARLN+T+R   N  P  G
Sbjct: 121 ATLDVVRNEIADVNARLNITIRAIANQAPAGG 152

BLAST of Lag0018185 vs. ExPASy TrEMBL
Match: A0A5A7T3M3 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold277G002020 PE=4 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 4.8e-35
Identity = 89/154 (57.79%), Postives = 115/154 (74.68%), Query Frame = 0

Query: 1   MSSNKQLTKTHVEHLVEIEEQLLYLREVPDAVRFVESRLEEISIKADKVDAVAGRVDGMP 60
           MSS+  L K   + LVE+EEQ+LYL EVPD++R++ESRLEEIS K + +DAVAGRV+G P
Sbjct: 1   MSSSNPLGKAQKDRLVELEEQMLYLVEVPDSIRYLESRLEEISEKTNTIDAVAGRVEGFP 60

Query: 61  IQKLMMRVENLESKAT--RPSSYERGDSSSGSAALMEERVDSLDSSQKVIVQMVTDLSED 120
           IQ+LM RV+ LE+     R  +YERGDSS+GS A +EERV  LDSSQK  ++M+  +SED
Sbjct: 61  IQELMTRVDALETTVNIGRTVNYERGDSSTGSVAHIEERVQELDSSQKTRLEMINGMSED 120

Query: 121 VKAALDVVRAEVADLSARLNLTMRVRKNLPPRRG 153
            +A LDVVR E+AD++ARL+LTMR   N  P  G
Sbjct: 121 FRATLDVVRNEIADVNARLSLTMRAMANQAPAGG 154

BLAST of Lag0018185 vs. TAIR 10
Match: AT4G23160.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 8 )

HSP 1 Score: 43.5 bits (101), Expect = 4.3e-04
Identity = 24/77 (31.17%), Postives = 41/77 (53.25%), Query Frame = 0

Query: 302 VGRYQSNPGLDHWGAVKNILKYLRRMRDYMLVYGAK-DLILTGYIDSDFQADKDSRKSTS 361
           + ++   P L H  AV  IL Y++      L Y ++ ++ L  + D+ FQ+ KD+R+ST+
Sbjct: 402 LSQFSEAPRLAHQQAVMKILHYIKGTVGQGLFYSSQAEMQLQVFSDASFQSCKDTRRSTN 461

Query: 362 GSIFTLNGGAVVWRSIK 378
           G    L    + W+S K
Sbjct: 462 GYCMFLGTSLISWKSKK 478

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK03099.12.6e-3557.79reverse transcriptase [Cucumis melo var. makuwa][more]
TYK03099.11.7e-2691.18reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0067252.15.8e-3557.79uncharacterized protein E6C27_scaffold418G00690 [Cucumis melo var. makuwa] >TYK1... [more]
KAA0046343.17.6e-3555.92uncharacterized protein E6C27_scaffold149G00520 [Cucumis melo var. makuwa] >TYK3... [more]
KAA0065409.11.0e-3454.76reverse transcriptase [Cucumis melo var. makuwa][more]

Pages

Match NameE-valueIdentityDescription
P109781.4e-1550.65Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
P0CV722.5e-1244.05Secreted RxLR effector protein 161 OS=Plasmopara viticola OX=143451 GN=RXLR161 P... [more]
Q993151.5e-0432.89Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20... [more]
Q7LHG51.5e-0432.89Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20... [more]
Q9ZT942.5e-0433.77Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A5D3DHI21.3e-3557.79Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A5D3BYE61.3e-3557.79Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold37... [more]
A0A5D3BYE68.2e-2791.18Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold37... [more]
A0A5D3E3553.7e-3555.92Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5A7T3M34.8e-3557.79Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27... [more]

Pages

Match NameE-valueIdentityDescription
AT4G23160.14.3e-0431.17cysteine-rich RLK (RECEPTOR-like protein kinase) 8 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.10.10HIV Type 1 Reverse Transcriptase, subunit A, domain 1coord: 144..260
e-value: 1.9E-15
score: 59.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 73..87
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 72..91
NoneNo IPR availablePANTHERPTHR24559TRANSPOSON TY3-I GAG-POL POLYPROTEINcoord: 145..240
NoneNo IPR availablePANTHERPTHR24559:SF330RNA-DIRECTED DNA POLYMERASE HOMOLOGcoord: 145..240
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 142..212

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0018185.1Lag0018185.1mRNA