Lag0018051 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0018051
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPyruvate kinase
Locationchr5: 14828098 .. 14833251 (-)
RNA-Seq ExpressionLag0018051
SyntenyLag0018051
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGTTCACGTCCACGTGCCGTTTTCTTTGTCTTCGCGCAGCAAGGTCGCACGCCTCCGTTTTCTTCTTTACACAGCCCAGTTTTCTTCGTCGTGCAGCCTTCTTTTTTGGTAGGTTCAGCCTTCACCCACTTGATTTCTTCATTTTTCAGCTTATGCTCATGTAGTTTTAGACTTCGAAATTGTTCTAGATCATGATCTATGCTTATTTGACTTGGAAATCGGATTACCCACTTTTTAGTAGCTTCAATTTTAAAGGTTTATGGTTCTTTGAAAGCCAACCAGCGAGCTTTTGGGCTCGAAATTTGTGTTCGACGAATACCCATAATCCGAACTGTCTTGAATCGAGTTACTCATAACCCGTAGACTTGGAAACTTAGTTGTTGGGGTTAGCAGAAAATTGGATCACTTTAAAATATTATTCTTGTGTGATGTCGATTGGAGATGAGGGTCTTCAAGATGACATTTTACTTTAGTTAGAAGTATAAGTTTAATGGTCTCGATCCTTGGTCCTGATGGTGACTGTGTCAAATATATGGACTATGTGATATTGAGGAGGTTTTGCTGCTCAATTTTTAAGTTTTGGATTGATCAACAGGGCTTACTTGATGATGAATGTTTTTTTTTCCTGCATCAGAACCAAATGATTTAGCTGTCAATATTTTTGTAGTAGAGATTGGTACATCTTTTATTCAAATGCATAACACAATTTAGACCTTTGCTTCTAACAATCGAATGAAATCTTCATTCTCTATATATGTAGCGTCCGCAAATTAAGGGAAAATTCAATTTTCTTTTAATTGGGTATTAAATTCGGCTGAGAGGGAAATTGAAATTTGAAAATTTGGAGAAAATTTAAAAGATGAGCTGGAGGGGCATTTTTGTAGATATTGAGATTGCTGAGCCCAAAGCCCAAGCCCAAGATCCCTTCTTTTAGTTGTGCACGCAGCCCTAGGGCTTAGCCATCTAGCTTCCATCTTCTCCTTCTCTTCTCCTTTAACCCACGACCGCAGCCTCATTCCCTTCTTCTTCTTCTTCAATTTGAGCAGTTGTCGGCGGCAGGAGCTCCGTCACGCGTCGTCCGACAGCGACTTCTTCTCCAGCAGGCGCGGCGGTTCGACGTTAGCAGCAGTTTCCGGCGACTGCCCTCGGTTCCGTCGGCAGCAGCAGTTTTCCGGCGGCTGGGTTGTGTAGGAGCGGCGGTAAGCTTGCTTTTTGGGTAATTTTGGAGTGGTTAAGTGCTCTTAGGGTGAAGTTTGACTAAATTATCTTGTTGTGTGGAATACCCATTGATTGTGGCTTTAGTTATAAAACCTTAAGCGAGTTCAAGGCGATTTTCGACGGAGTTTGTGTGGGTATATTCCTTTGGCGTTTTCGGCGAATTTAGCTTTGGGTAAGTGAGATTTAAGATTATTTGGACCAATTTCACTCACTAATCAAATACCCATTAATTCTAGTTTTCAATTTGAAGGTACCCATAGCGTTGGAGGTTTAATTGTCGAGTTGGAGCGATAATAGAATCCGACCAGCAAGCTTAGATTCTGTTAAGGTTGAGTTTGACCCTTTGTTGAGTTGTTTAGGCGGCGTAAAATACAGACCAAAGGTAAGTGAACTTACTGTTGAACCCTTTTAATTGGGTGCGAATATTTGAGTTCCTAAAATTCTTGTGTTTAGAATAATTGTTTTCTATAATATTCGTGTTTATGAGTTGCTCTTGAGATTATTATTATTGTTTAAAAGCATGAGAATTATGCATAATGTATGTTTATTATGCATGAGTTTATGAAAATTATCGAAAGCATGAAAATTAGTATTTGCTTATTAAAATAGTTGAGATGGTGGTCTCTTGAAATTCCATCAAATGAATTAGTTTAAATTATTTATAAGATTAAATTAGCGTGCATGAAATTGATGTGCGTTCAGAGATGCCTACGCAGGACTTAGGGACATGAAATTGATGCACCTAAGTCGAGGGTTGTGAAATTGACGCCCTCGAGGTTTCGTGAAATTGACGGCCTCTGTAGGAGACATATGAAATTGATGTGTCTAGAATATGTATGAAAGTGATGCATATTCTCTCTCATTAGAAGTACACATGAAATTGATGTGTTTTAGGATAGGCATGAAATTGATGTGTATCCTAAGCTTAAAATAAAGTTAAGAGCCACAAAAATTATATTTGATGTCTTGGTTTTTAAAATGTTTTTAAAGCGTGACCCAACCTCAATAGTAGCGTCACTTACTGAGTATTTAAAATACTCATTCTTTTCAAAAATGTTTTCTTTTCAGGTAAGGACAAGGGCAGATGAAAGGCGATTGATGAGAAATCGATAGATGGCCATGGGAGGACTCGAGTTCATGAATTATTTTATTCCGCGTTAAGAACCATGTTTTGTTTTTTGTAAATTGTTTTAAGAAGATTTTGTTTTTAGAGGCATTGTACATTTATGTTTTTAGAAATTTTTGAGTTTATTTAAAACCTTTGAAATTAGGGATCCCGAATAAAAAGTTTTGCTTTAAAGTTTTTGTTTAAATTAGTGTTTCTGTTTCCAATTATTTTTATTTAAAAATGTCTCCAAAGCATGTATTGTCGTAACGGTCCCTTAAAGACTTATAAAAAAAGTCGGGTCGTTACAATATAATTTTGTCATTCAGTTGCATTGAGAATTTTCTATTTGGTGCCCCACGATTAAGATGAGGTACACTTGTGTACAACTATATTTACCTATTGATCTGCTTTTTACTAGTTGAAATATAAATCTAGTTATTTATCACTTTTAGCTGATGAGATATAGAATTGCAATTGCCTATTCTAGTGTCTCACAACAAAATTTCTTTTCCATACTCTAGGTGGAATGATGTCACTGGCTGTTAAGTCAAAGACAGATGATTTGGTTAAGTGTGTAGTCATTGATGGCGGTGAACTCAAATCGAGGCGACATTTGAATGTTCGTGGAAAAAGCGTGACATTGCCTTCTATAATGGGTATGTTCAGTATTTAACATCAATGTTGCATTATAGAATTTAGGTTAAATTACAATTCTAGTCTTGCTATTTTGACACTTTTAATGTCCAGTCTTCCTTTATTTTTATTTTAGCCTTGCTACTATGTCAGCTTTTTGTTACCTATTTAAATGGATAATAGACTTGAGGGACTATTGGTACGTCCATAGAACATGTACCAAAATGAAACAAATGAAAGGTGAGACCAGAAGACCAATGAACATGAATTGACATTTTAAATAACTTAGACTACTTATATATTTAATATTTGACTGTGTTTGTTTACAATCTAGAAATGTAATTGTATTACTATTGGCAACTATCTTTTACGTGATATTGATTTGAACTTATGCACGATGATTTCTAGCTACCAAGTTATTTCCTAATCTATTACATTTTGAAGTTGCATTTATTTATTCATTTATGCTAATACATTCTTGGCTCATTTCATTGTGTCACATGTTACTGTTCATAATCATCATGGTGTTCTGAGTTACAACTTAACTAGTTTATTAGACTTTTTAGGCAATGGTTGCTCGTGGAGACCTTGGGGCTGAACTTCCAATTGTGGAAGTTCCATTATTGCAGGTAAAACTCTAACGTTATTACTACTCTCTCATACATCACTCTTCCATTGTGCAACATTAGTTCCAGATTAACTTTTTTAAACTTAAATGGTTTGTGTTTACATAAAAAAGATTACAATTGGCGTTGATCATAAATTAATTATAATTATACAAAATTATAGTCAAAGTGAACATAATTCAGTGTTAATTGACATGTACTCCTGACTAAGAGGTCAGATGTTTGAATCTCACACCTCCAGATGTTGATGAACTCAAGAAAAAAAAAAGAATTAAATGAAAATGTTTTTAGGAGAAACTACTCATGGAATGTATGCATCTTATTCCATTATCTAGGCAGTTTTATTTTTGGTGGCAGCAATTCTGTTTATTTTTTGAATGAAAATGTTTTATTCAAAACCTAGTTTGTCCCCATGGGTCATTTTGGTACTGAACGAGGCCTTTTGATCTTTTAATAACATAGAAGGCAATACATATGAAGATGTTTTTGTTTGTTGTTGTAATTGCGTTTTGCAAATATTGAAATATGCCATATCCCGCATGCTTTATGCTCCTGTTCGTTTTTCTAATTTGATTGGAATTGGTGAAAGAGTTCCAACATTCTCGATGTCACGACATCTCGTTCCAACTTTTTGTGTCTACTAGTTCGGCAATTGGACTTCAAGTTATTGCTAAATATTGAAGAAGTTAACGTTGTTTTCTGTTTGCTGATGTTCTAAAAATTACTCCTTTTTTTCCCTTCTTGACGTAGTGTTTATATCAAATTGAACTTTTGGTCCTAATGGTGAAAATAGGCATCCATTGAAGGCTGTGAAAGTGATGCATTGGTTCCAAATGCACTATTGGAATTTGTCTTTTGGTGATGTATACTAGCACAATGTCTTTATAGTTTTGGAGTGACCAATACTGTTATACTGATATAGATTGATGCTTTTGCATATTGTAAAAGAATTTGGTTAGATGTGTGGAAGAGGTTTGTGGGTTTAGGTTATTTACCAATTTCTCTTTAGCATTATCATTGTTACTCAATAAACATGCTATGGGTTTTTGTGTGATAGAATGTAATTTATATGTCCTTTTTCTCTACAAATTTGAAGTGGTTTCTATTGTTTCTGCCAATATGGTCAATATCAAGTATTGATTGTATTTAGATTTATGCTTATGAGTATCTTCTTTGGTCCCCAACAATTTTATTCTTGTGAGTATCTTCTTTTCCAGTCTTGGATCCATTTGTGCAAAATAATTGAGTTGAGAAAACATATGATTTGGAGTTACTGTTTCATGTTTACTGTATTTGGATCATTTGTTCATGTAATTCTACACTTGCTTTTACATTTTCAGGTGCCTTTCCTCAGTTTTGAAGTACTTCAATTTTGTAGAGTGTAGTTGTTTATTCATTATCTCCTTTTTTTTTCTCCTCACATCTTTGACAAATTGGATTATATTTGAGGGATTGTGATTATCCAATTCACAATGAGGATTATTTGCATTCTAATACTAATTCTTTTCTTCGGCAGGAGTTCCTAAATTGGAGATGGTGCAAATGTATCCACAAGACTTGATGTGA

mRNA sequence

ATGCGTTCACGTCCACGTGCCGTTTTCTTTGTCTTCGCGCAGCAAGGTCGCACGCCTCCGTTTTCTTCTTTACACAGCCCAGTTTTCTTCGTCGTGCAGCCTTCTTTTTTGTTGTCGGCGGCAGGAGCTCCGTCACGCGTCGTCCGACAGCGACTTCTTCTCCAGCAGGCGCGGCGGTTCGACGTTAGCAGCAGTTTCCGGCGACTGCCCTCGGTTCCGTCGGCAGCAGCAGTTTTCCGGCGGCTGGGTTGTGTAGGAGCGGCGGTAAGCTTGCTTTTTGGTTATAAAACCTTAAGCGAGTTCAAGGCGATTTTCGACGGAGTTTGTGTGGGTGGAATGATGTCACTGGCTGTTAAGTCAAAGACAGATGATTTGGTTAAGTGTGTAGTCATTGATGGCGGTGAACTCAAATCGAGGCGACATTTGAATGTTCGTGGAAAAAGCGTGACATTGCCTTCTATAATGGGAGTTCCTAAATTGGAGATGGTGCAAATGTATCCACAAGACTTGATGTGA

Coding sequence (CDS)

ATGCGTTCACGTCCACGTGCCGTTTTCTTTGTCTTCGCGCAGCAAGGTCGCACGCCTCCGTTTTCTTCTTTACACAGCCCAGTTTTCTTCGTCGTGCAGCCTTCTTTTTTGTTGTCGGCGGCAGGAGCTCCGTCACGCGTCGTCCGACAGCGACTTCTTCTCCAGCAGGCGCGGCGGTTCGACGTTAGCAGCAGTTTCCGGCGACTGCCCTCGGTTCCGTCGGCAGCAGCAGTTTTCCGGCGGCTGGGTTGTGTAGGAGCGGCGGTAAGCTTGCTTTTTGGTTATAAAACCTTAAGCGAGTTCAAGGCGATTTTCGACGGAGTTTGTGTGGGTGGAATGATGTCACTGGCTGTTAAGTCAAAGACAGATGATTTGGTTAAGTGTGTAGTCATTGATGGCGGTGAACTCAAATCGAGGCGACATTTGAATGTTCGTGGAAAAAGCGTGACATTGCCTTCTATAATGGGAGTTCCTAAATTGGAGATGGTGCAAATGTATCCACAAGACTTGATGTGA

Protein sequence

MRSRPRAVFFVFAQQGRTPPFSSLHSPVFFVVQPSFLLSAAGAPSRVVRQRLLLQQARRFDVSSSFRRLPSVPSAAAVFRRLGCVGAAVSLLFGYKTLSEFKAIFDGVCVGGMMSLAVKSKTDDLVKCVVIDGGELKSRRHLNVRGKSVTLPSIMGVPKLEMVQMYPQDLM
Homology
[Close]
BLAST of Lag0018051 vs. NCBI nr
Match: KAF3449478.1 (hypothetical protein FNV43_RR10206 [Rhamnella rubrinervis])

HSP 1 Score: 87.0 bits (214), Expect = 1.7e-13
Identity = 41/44 (93.18%), Postives = 43/44 (97.73%), Query Frame = 0 

Query: 111 GGMMSLAVKSKTDDLVKCVVIDGGELKSRRHLNVRGKSVTLPSI 155
           GGMMSLAVKSKTDD+VKCVV+DGGELKSRRHLNVRGKS TLPSI
Sbjct: 226 GGMMSLAVKSKTDDVVKCVVVDGGELKSRRHLNVRGKSATLPSI 269

[Close]
BLAST of Lag0018051 vs. NCBI nr
Match: XP_022931063.1 (pyruvate kinase isozyme G, chloroplastic-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 86.7 bits (213), Expect = 2.2e-13
Identity = 42/44 (95.45%), Postives = 42/44 (95.45%), Query Frame = 0 

Query: 111 GGMMSLAVKSKTDDLVKCVVIDGGELKSRRHLNVRGKSVTLPSI 155
           GGMMSLAVKSKTDD VKCVVIDGGELKSRRHLNVRGKS TLPSI
Sbjct: 225 GGMMSLAVKSKTDDTVKCVVIDGGELKSRRHLNVRGKSATLPSI 268

[Close]
BLAST of Lag0018051 vs. NCBI nr
Match: XP_022931060.1 (pyruvate kinase isozyme G, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022931061.1 pyruvate kinase isozyme G, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 86.7 bits (213), Expect = 2.2e-13
Identity = 42/44 (95.45%), Postives = 42/44 (95.45%), Query Frame = 0 

Query: 111 GGMMSLAVKSKTDDLVKCVVIDGGELKSRRHLNVRGKSVTLPSI 155
           GGMMSLAVKSKTDD VKCVVIDGGELKSRRHLNVRGKS TLPSI
Sbjct: 225 GGMMSLAVKSKTDDTVKCVVIDGGELKSRRHLNVRGKSATLPSI 268

[Close]
BLAST of Lag0018051 vs. NCBI nr
Match: KAG7036175.1 (hypothetical protein SDJN02_02976 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 86.7 bits (213), Expect = 2.2e-13
Identity = 42/44 (95.45%), Postives = 42/44 (95.45%), Query Frame = 0 

Query: 111 GGMMSLAVKSKTDDLVKCVVIDGGELKSRRHLNVRGKSVTLPSI 155
           GGMMSLAVKSKTDD VKCVVIDGGELKSRRHLNVRGKS TLPSI
Sbjct: 225 GGMMSLAVKSKTDDTVKCVVIDGGELKSRRHLNVRGKSATLPSI 268

[Close]
BLAST of Lag0018051 vs. NCBI nr
Match: XP_022931062.1 (pyruvate kinase isozyme G, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 86.7 bits (213), Expect = 2.2e-13
Identity = 42/44 (95.45%), Postives = 42/44 (95.45%), Query Frame = 0 

Query: 111 GGMMSLAVKSKTDDLVKCVVIDGGELKSRRHLNVRGKSVTLPSI 155
           GGMMSLAVKSKTDD VKCVVIDGGELKSRRHLNVRGKS TLPSI
Sbjct: 138 GGMMSLAVKSKTDDTVKCVVIDGGELKSRRHLNVRGKSATLPSI 181

[Close]
BLAST of Lag0018051 vs. ExPASy Swiss-Prot
Match: Q93Z53 (Plastidial pyruvate kinase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP3 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.1e-15
Identity = 41/44 (93.18%), Postives = 42/44 (95.45%), Query Frame = 0 

Query: 111 GGMMSLAVKSKTDDLVKCVVIDGGELKSRRHLNVRGKSVTLPSI 155
           GGMMSLAVKSKT DLVKCVVIDGGEL+SRRHLNVRGKS TLPSI
Sbjct: 222 GGMMSLAVKSKTSDLVKCVVIDGGELQSRRHLNVRGKSATLPSI 265

[Close]
BLAST of Lag0018051 vs. ExPASy Swiss-Prot
Match: P55964 (Pyruvate kinase isozyme G, chloroplastic (Fragment) OS=Ricinus communis OX=3988 PE=2 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.4e-15
Identity = 40/44 (90.91%), Postives = 41/44 (93.18%), Query Frame = 0 

Query: 111 GGMMSLAVKSKTDDLVKCVVIDGGELKSRRHLNVRGKSVTLPSI 155
           GGMMSLAVKSKT DLVKCVV+DGGELKSRRHLNVRGKS  LPSI
Sbjct: 71  GGMMSLAVKSKTSDLVKCVVVDGGELKSRRHLNVRGKSARLPSI 114

[Close]
BLAST of Lag0018051 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 3.2e-15
Identity = 40/44 (90.91%), Postives = 41/44 (93.18%), Query Frame = 0 

Query: 111 GGMMSLAVKSKTDDLVKCVVIDGGELKSRRHLNVRGKSVTLPSI 155
           GGMMSLAVKSKT D+VKC VIDGGELKSRRHLNVRGKS TLPSI
Sbjct: 214 GGMMSLAVKSKTSDIVKCEVIDGGELKSRRHLNVRGKSATLPSI 257

[Close]
BLAST of Lag0018051 vs. ExPASy Swiss-Prot
Match: Q9FLW9 (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.7e-13
Identity = 37/44 (84.09%), Postives = 38/44 (86.36%), Query Frame = 0 

Query: 111 GGMMSLAVKSKTDDLVKCVVIDGGELKSRRHLNVRGKSVTLPSI 155
           GGMMS  VKSKT D VKC V+DGGELKSRRHLNVRGKS TLPSI
Sbjct: 233 GGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLNVRGKSATLPSI 276

The following BLAST results are available for this feature:
BLAST of Lag0018051 vs. NCBI nr
Match NameE-valueIdentityDescription
KAF3449478.11.7e-1393.18hypothetical protein FNV43_RR10206 [Rhamnella rubrinervis][more]
XP_022931063.12.2e-1395.45pyruvate kinase isozyme G, chloroplastic-like isoform X3 [Cucurbita moschata][more]
XP_022931060.12.2e-1395.45pyruvate kinase isozyme G, chloroplastic-like isoform X1 [Cucurbita moschata] >X... [more]
KAG7036175.12.2e-1395.45hypothetical protein SDJN02_02976 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022931062.12.2e-1395.45pyruvate kinase isozyme G, chloroplastic-like isoform X2 [Cucurbita moschata][more]
BLAST of Lag0018051 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q93Z531.1e-1593.18Plastidial pyruvate kinase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P... [more]
P559642.4e-1590.91Pyruvate kinase isozyme G, chloroplastic (Fragment) OS=Ricinus communis OX=3988 ... [more]
Q405463.2e-1590.91Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9FLW91.7e-1384.09Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015806Pyruvate kinase, insert domain superfamilyGENE3D2.40.33.10coord: 108..152
e-value: 1.9E-7
score: 32.4
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 109..155
NoneNo IPR availablePANTHERPTHR11817:SF2PLASTIDIAL PYRUVATE KINASE 2coord: 109..155
IPR011037Pyruvate kinase-like, insert domain superfamilySUPERFAMILY50800PK beta-barrel domain-likecoord: 111..150

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0018051.1Lag0018051.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity