Lag0017773 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0017773 vs. NCBI nr
Match: XP_022143530.1 (valencene synthase-like isoform X2 [Momordica charantia])

HSP 1 Score: 262.7 bits (670), Expect = 2.4e-66
Identity = 133/178 (74.72%), Postives = 149/178 (83.71%), Query Frame = 0

Query: 1   MVRKQLHELKEKVRSMLAVAEKPSEKLGLIDSIQRLGLSYHFEGEITEILHHFQN-PCNV 60
           MVRKQL E +E+VR ML  AEK SEKL LIDSIQRLG+SYHFEGEI EIL H QN  CN+
Sbjct: 44  MVRKQLEEQREEVRRMLEAAEKCSEKLSLIDSIQRLGISYHFEGEINEILEHMQNDSCNL 103

Query: 61  DDINDENLYVIALRFRLLRQQGYHVSCEIFNKFTNEKGDLKESVAKDESGILNLYEASHL 120
           DD  DE++Y++ALRFRLLRQQGY VSCEIFN+FTN  GD KE++ KDE GIL+LYEASHL
Sbjct: 104 DD--DEDIYIVALRFRLLRQQGYSVSCEIFNRFTNGDGDFKETMLKDECGILSLYEASHL 163

Query: 121 RMKGENILDEALAFSTIHLQALVMDYKSPFSDEAKYALKWPIYKDLPRFMTWHYISLY 178
           RM GENILD+ALAF+T HLQA+ M   SPFS+EAKYALKWPIYK LPRF T HYISLY
Sbjct: 164 RMNGENILDKALAFTTTHLQAMAMSSSSPFSEEAKYALKWPIYKALPRFTTRHYISLY 219

BLAST of Lag0017773 vs. NCBI nr
Match: XP_022143529.1 ((-)-germacrene D synthase-like isoform X1 [Momordica charantia])

HSP 1 Score: 262.7 bits (670), Expect = 2.4e-66
Identity = 133/178 (74.72%), Postives = 149/178 (83.71%), Query Frame = 0

Query: 1   MVRKQLHELKEKVRSMLAVAEKPSEKLGLIDSIQRLGLSYHFEGEITEILHHFQN-PCNV 60
           MVRKQL E +E+VR ML  AEK SEKL LIDSIQRLG+SYHFEGEI EIL H QN  CN+
Sbjct: 44  MVRKQLEEQREEVRRMLEAAEKCSEKLSLIDSIQRLGISYHFEGEINEILEHMQNDSCNL 103

Query: 61  DDINDENLYVIALRFRLLRQQGYHVSCEIFNKFTNEKGDLKESVAKDESGILNLYEASHL 120
           DD  DE++Y++ALRFRLLRQQGY VSCEIFN+FTN  GD KE++ KDE GIL+LYEASHL
Sbjct: 104 DD--DEDIYIVALRFRLLRQQGYSVSCEIFNRFTNGDGDFKETMLKDECGILSLYEASHL 163

Query: 121 RMKGENILDEALAFSTIHLQALVMDYKSPFSDEAKYALKWPIYKDLPRFMTWHYISLY 178
           RM GENILD+ALAF+T HLQA+ M   SPFS+EAKYALKWPIYK LPRF T HYISLY
Sbjct: 164 RMNGENILDKALAFTTTHLQAMAMSSSSPFSEEAKYALKWPIYKALPRFTTRHYISLY 219

BLAST of Lag0017773 vs. NCBI nr
Match: XP_031738277.1 ((-)-germacrene D synthase isoform X2 [Cucumis sativus])

HSP 1 Score: 260.8 bits (665), Expect = 9.0e-66
Identity = 129/179 (72.07%), Postives = 150/179 (83.80%), Query Frame = 0

Query: 1   MVRKQLHELKEKVRSMLAVAEKPSEKLGLIDSIQRLGLSYHFEGEITEILHHFQNPCNVD 60
           M+RK+L ELKE+VRSM     K S+KL LIDSIQRLGLSYHF+ EI E+L   +NPCNVD
Sbjct: 57  MIRKKLQELKEEVRSMFVTTNKFSKKLSLIDSIQRLGLSYHFDDEINEVLMLMKNPCNVD 116

Query: 61  DINDENLYVIALRFRLLRQQGYHVSCEIFNKFTNEKGDLKESVAKDESGILNLYEASHLR 120
           D   E+LY+ ALRFRLLRQQG+ VSCEIFNKFTNE+G+LKES+ KDE+G+L+LYEASHLR
Sbjct: 117 D-EHEDLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRKDENGLLSLYEASHLR 176

Query: 121 MKGENILDEALAFSTIHLQALVMDYKSPFSDEAKYALKWPIYKDLPRFMTWHYISLYHN 180
           MKGENILDEALAF+T HL+A+ MD  SPF +EAKYALKWPIYK + RFM  HYISLY N
Sbjct: 177 MKGENILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYSN 234

BLAST of Lag0017773 vs. NCBI nr
Match: XP_038877743.1 (LOW QUALITY PROTEIN: (-)-germacrene D synthase-like [Benincasa hispida])

HSP 1 Score: 256.9 bits (655), Expect = 1.3e-64
Identity = 127/179 (70.95%), Postives = 148/179 (82.68%), Query Frame = 0

Query: 1   MVRKQLHELKEKVRSMLAVAEKPSEKLGLIDSIQRLGLSYHFEGEITEILHHFQNPCNVD 60
           MV KQ+ E KE+VR M  VAEK SEKL LIDSIQRLGLSYHF+ E +E++ + QNP NVD
Sbjct: 51  MVMKQVQEYKEEVRRMFGVAEKYSEKLSLIDSIQRLGLSYHFKDESSEVIDYIQNPTNVD 110

Query: 61  DINDENLYVIALRFRLLRQQGYHVSCEIFNKFTNEKGDLKESVAKDESGILNLYEASHLR 120
           +  DE+LY+ ALRFRLLRQQG+ VSC+IFNKFTNE GD KES+ KD +G ++LYEASHLR
Sbjct: 111 E-EDEDLYITALRFRLLRQQGFFVSCDIFNKFTNEMGDFKESIVKDGAGXISLYEASHLR 170

Query: 121 MKGENILDEALAFSTIHLQALVMDYKSPFSDEAKYALKWPIYKDLPRFMTWHYISLYHN 180
           MKGENILDEALAF+T  L+A+ MD  SPFS+EAKYALKWPIYK +PRFM  HYISLY N
Sbjct: 171 MKGENILDEALAFTTTQLKAMAMDSSSPFSEEAKYALKWPIYKAMPRFMNRHYISLYQN 228

BLAST of Lag0017773 vs. NCBI nr
Match: XP_022143526.1 ((-)-germacrene D synthase-like isoform X1 [Momordica charantia])

HSP 1 Score: 255.8 bits (652), Expect = 2.9e-64
Identity = 129/178 (72.47%), Postives = 147/178 (82.58%), Query Frame = 0

Query: 1   MVRKQLHELKEKVRSMLAVAEKPSEKLGLIDSIQRLGLSYHFEGEITEILHHFQN-PCNV 60
           +VRKQL E KE VR M+  A+K SEKL LIDSIQRLGLSYHFEGEI EIL H QN  CN+
Sbjct: 49  VVRKQLEEQKEGVRRMVEAADKCSEKLSLIDSIQRLGLSYHFEGEINEILEHMQNDSCNL 108

Query: 61  DDINDENLYVIALRFRLLRQQGYHVSCEIFNKFTNEKGDLKESVAKDESGILNLYEASHL 120
           DD  DE++Y++ALRFRLLRQQGY VSCEIFN+FT E GD KES+ KDE GIL+LY+ASHL
Sbjct: 109 DD--DEDIYIVALRFRLLRQQGYSVSCEIFNRFTTENGDFKESMVKDECGILSLYQASHL 168

Query: 121 RMKGENILDEALAFSTIHLQALVMDYKSPFSDEAKYALKWPIYKDLPRFMTWHYISLY 178
            M GENILD+ALAF+T HLQA+ MD  SPF++EAKYALKWPIYK +PR  T HYISLY
Sbjct: 169 GMNGENILDKALAFTTTHLQAMAMDSSSPFAEEAKYALKWPIYKAMPRXTTRHYISLY 224

BLAST of Lag0017773 vs. ExPASy Swiss-Prot
Match: Q6Q3H3 ((-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.7e-43
Identity = 90/177 (50.85%), Postives = 127/177 (71.75%), Query Frame = 0

Query: 2   VRKQLHELKEKVRSMLAVAEKPS-EKLGLIDSIQRLGLSYHFEGEITEILHHFQNPCNVD 61
           +++ + +LKE+VR ML  A+  S +KL LID+IQRLG++YHFE EI E+L H  +   V 
Sbjct: 50  LKQHVQQLKEEVRKMLMAADDDSAQKLLLIDAIQRLGVAYHFESEIDEVLKHMFDGSVVS 109

Query: 62  DINDENLYVIALRFRLLRQQGYHVSCEIFNKFTNEKGDLKESVAKDESGILNLYEASHLR 121
              +E++Y  +LRFRLLRQQGYHVSC++FN F + +G+ KES++ D  G+L+LYEA+H R
Sbjct: 110 --AEEDVYTASLRFRLLRQQGYHVSCDLFNNFKDNEGNFKESLSSDVRGMLSLYEATHFR 169

Query: 122 MKGENILDEALAFSTIHLQALVMDYKSPFSDEAKYALKWPIYKDLPRFMTWHYISLY 178
           + GE+ILDEALAF+T HLQ+      +P +++  +ALK PI K LPR    HY S+Y
Sbjct: 170 VHGEDILDEALAFTTTHLQSATKHSSNPLAEQVVHALKQPIRKGLPRLEARHYFSVY 224

BLAST of Lag0017773 vs. ExPASy Swiss-Prot
Match: B9SBV6 (Probable terpene synthase 3 OS=Ricinus communis OX=3988 GN=TPS3 PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 5.2e-40
Identity = 88/176 (50.00%), Postives = 123/176 (69.89%), Query Frame = 0

Query: 3   RKQLHELKEKVRSM-LAVAEKPSEKLGLIDSIQRLGLSYHFEGEITEILHHFQNPCNVDD 62
           ++Q   L+E+VR M ++  +KPS KL LID IQRLG++YHFE EI E++    +  N DD
Sbjct: 43  KEQAKVLEEEVRRMIISETQKPSGKLKLIDVIQRLGIAYHFEEEIGEVIEQVYS--NYDD 102

Query: 63  INDENLYVIALRFRLLRQQGYHVSCEIFNKFTNEKGDLKESVAKDESGILNLYEASHLRM 122
             DE+LY IALRFRLLRQQGY+VS ++F+KF + KGD K+ +  D  G+L+L+EAS++ +
Sbjct: 103 --DEDLYDIALRFRLLRQQGYNVSSDVFDKFKDCKGDFKKHLVNDVQGLLSLHEASYMSV 162

Query: 123 KGENILDEALAFSTIHLQALVMDYKSPFSDEAKYALKWPIYKDLPRFMTWHYISLY 178
           +GE ILDEAL F+  HL A  +   SP  D+  +AL+WP+ + LPR   W Y S+Y
Sbjct: 163 QGEKILDEALEFTKTHLMATQL--SSPLPDQVSHALRWPVRRGLPRKEAWQYFSIY 212

BLAST of Lag0017773 vs. ExPASy Swiss-Prot
Match: B9SCB6 (Probable terpene synthase 2 OS=Ricinus communis OX=3988 GN=TPS2 PE=3 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 7.4e-39
Identity = 81/176 (46.02%), Postives = 121/176 (68.75%), Query Frame = 0

Query: 2   VRKQLHELKEKVRSMLAVAEKPSEKLGLIDSIQRLGLSYHFEGEITEILHHFQNPCNVDD 61
           + +Q+  LKE+V+ M+A A++PS+ L LID +QRLG+SYHFE EI E L    N  N D 
Sbjct: 45  MEQQVERLKEEVKKMIASADEPSQILNLIDLLQRLGVSYHFEKEIEEALQQVLN-MNSDS 104

Query: 62  INDENLYVIALRFRLLRQQGYHVSCEIFNKFTNEKGDLKESVAKDESGILNLYEASHLRM 121
             D++L+ +ALRFRLLR+QG +VSC++FNKF +  G   +++  D  G+L+LYEA+H R+
Sbjct: 105 DKDDDLHSVALRFRLLREQGLNVSCDVFNKFRDRNGHFIQTLKTDLQGMLSLYEAAHFRV 164

Query: 122 KGENILDEALAFSTIHLQALVMDYKSPFSDEAKYALKWPIYKDLPRFMTWHYISLY 178
            GE ILD+ALAF+T +L+++V +   P + +    L+ P+ K L R    H+IS+Y
Sbjct: 165 HGEGILDDALAFTTTYLESIVPNLSPPLAAQISRTLRQPLRKSLARVEARHFISIY 219

BLAST of Lag0017773 vs. ExPASy Swiss-Prot
Match: Q6Q3H2 (Valencene synthase OS=Vitis vinifera OX=29760 GN=ValCS PE=1 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 1.7e-38
Identity = 86/178 (48.31%), Postives = 120/178 (67.42%), Query Frame = 0

Query: 4   KQLHELKEKVRSML--AVAEKPSEKLGLIDSIQRLGLSYHFEGEITEILHHFQNPCNVDD 63
           +Q+ +LK++V+  L  A    PS+ L  ID++QRLG++YHFE EI E L H  N  +  +
Sbjct: 49  EQIEDLKKEVKRKLTAAAVANPSQLLNFIDAVQRLGVAYHFEQEIEEALQHICNSFHDCN 108

Query: 64  INDENLYVIALRFRLLRQQGYHVSCEIFNKFTNEKGDLKESVAKDESGILNLYEASHLRM 123
             D +LY IAL FRLLRQQGY +SC+IFNKFT+E+G  KE++  D  G+L LYEA+HLR+
Sbjct: 109 DMDGDLYNIALGFRLLRQQGYTISCDIFNKFTDERGRFKEALISDVRGMLGLYEAAHLRV 168

Query: 124 KGENILDEALAFSTIHLQALVMDYKSPFSDEAKYALKWPIYKDLPRFMTWHYISLYHN 180
            GE+IL +ALAF+T HL+A+V       +++  +AL  PI K L R     YIS+Y +
Sbjct: 169 HGEDILAKALAFTTTHLKAMVESLGYHLAEQVAHALNRPIRKGLERLEARWYISVYQD 226

BLAST of Lag0017773 vs. ExPASy Swiss-Prot
Match: O49853 ((+)-delta-cadinene synthase isozyme C2 OS=Gossypium arboreum OX=29729 GN=CAD1-C2 PE=2 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 2.8e-38
Identity = 86/177 (48.59%), Postives = 119/177 (67.23%), Query Frame = 0

Query: 4   KQLHELKEKVRSML-AVAEKPSEKLGLIDSIQRLGLSYHFEGEITEILHHFQNPCNVDDI 63
           K+  +LKE+VR M+ A     ++KL  IDS+QRLG+SYHF  EI + L +  +  N D  
Sbjct: 50  KRHQQLKEEVRKMIVAPMANSTQKLAFIDSVQRLGVSYHFTKEIEDELENIYHN-NNDAE 109

Query: 64  NDENLYVIALRFRLLRQQGYHVSCEIFNKFTNEKGDLKESVAKDESGILNLYEASHLRMK 123
           ND  LY  +LRFRLLR+ GY+VSC++FNKF +E+G+ K SV  D  G+L LY+AS+LR+ 
Sbjct: 110 ND--LYTTSLRFRLLREHGYNVSCDVFNKFKDEQGNFKSSVTSDVQGLLELYQASYLRVH 169

Query: 124 GENILDEALAFSTIHLQALVMDYKSPFSDEAKYALKWPIYKDLPRFMTWHYISLYHN 180
           GE+ILDEA++F+T HL   V     P S+E  +ALK  I + LPR    HY+S+Y +
Sbjct: 170 GEDILDEAISFTTNHLSLAVSSLDHPLSEEVSHALKQSIRRGLPRVEARHYLSVYQD 223