Lag0017733 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0017733
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCCHC-type domain-containing protein
Locationchr5: 8026822 .. 8028063 (-)
RNA-Seq ExpressionLag0017733
SyntenyLag0017733
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGGATCGAGGCCAGAAAGAAGGTCAAGTGCGTGAATCCATTACGTTAGTCGGTACTAAGCATAAGACAGAGGAGGTTCTGAACATCTAAGCAAGAAGAGGATAGTGGTGTCAGAGGAAGAATTAAATGCGAGATCGGTGGAGGCTACTGGATAGCCCGCGGGCACAATGAAAATCTTAAGCTGGAATGCCAGGGTTTGAGGAACCCTAGGACATTAAGGGTGCTACGACACTTAATTCGTACGCACCAACCCCAACTGGTTTTCTTGATGGAAACTAAGCTTAAAGGTAACGAGGGTGACAAAGTGATGAGACGGTTGCAGTTTGAGTATAGTTTGGTTGTTCCTAGTATTAGGTAGAGTGGGGGATTGATGTTACTATGGAGGAATGAGACTAAGGTTAGTATTCACTCATATTCGAAGGGGCATATAGATGCGACTATCCAGGAAGGAGGTTGGTCATGGCGGTTTACAGGCATTTATGGAAACCCAGTTAGAGGTTTGCATCATGAAACGTGGAGGTTAATGACTAGACTAGCTGAGCAGTCAGATATTCGTGGGTGCTGGGTGGTGACTTTAGTGAGATTACATATGGGTATGAGAAAAAGGTGGCTCTAGTAGGGTTGAAGCTGATATGAGAGACTTTTGAGAGGTTATAGATTGTTGTGGGGTCCTGGACCCTGGTTTTATTGGTATGAAATATACGTGGAGTAATAAGCACACCTCTCGGGCACCTATTTGGGAGAGGTTGGATCGCTTCCTGATTAGCCTACTATGAGACATCGTTGTAGTGTATTCAAAGTACACCATTTGGCTCTGGTAGCAACTGACCATAGACCTCTCCTAGCGGAATGGAAAGAAGAACCACCTGATATAAATCATAATGTAAGGCAGAGACCTCGACTAGATATAAAGAATGCAATGAGATAGTGAATCAAGTCTGGAAGAGTACTGAGTGGTATCCAGTTCGCAACATAACGGAGAAGACCAGGGAGTGTTTGTTGAGACTGCATTAGTGGAGTAGGCGACACTATGAAGGATCCATTAGAGGAGCTATTAATCATAAAGAAGTGGAAATTCAACACCTTCTCAGGCTTGGAGATGATCTTAGGGACAATGAGCTTAAGGAGAAGGAAAATGAGCTTGAAAATCTATTAGAGGATGATGAGATCTATTGGAAGCAGAGAACCCGGGAGGATTGGTTAACTTGGGTGACCGCAATACTAAATGGTTCCATCTGA

mRNA sequence

ATGATGGATCGAGGCCAGAAAGAAGGTCAAGTGCGTGAATCCATTACGTTAGTCGGTACTAAGCATAAGACAGAGGAGAGTGGGGGATTGATGTTACTATGGAGGAATGAGACTAAGGTTAGTATTCACTCATATTCGAAGGGGCATATAGATGCGACTATCCAGGAAGGAGGTTGGTCATGGCGGTTTACAGGCATTTATGGAAACCCAGTTAGAGGTTTGCATCATGAAACGTGGAGGTTAATGACTAGACTAGCTGAGCAGTCAGATATTCGTGGGTGCTGGGTGGTGACTTTAGTGAGATTACATATGGGTATGAGAAAAAGTGTATTCAAAGTACACCATTTGGCTCTGGTAGCAACTGACCATAGACCTCTCCTAGCGGAATGGAAAGAAGAACCACCTGATATAAATCATAATTGGAGTAGGCGACACTATGAAGGATCCATTAGAGGAGCTATTAATCATAAAGAAGTGGAAATTCAACACCTTCTCAGGCTTGGAGATGATCTTAGGGACAATGAGCTTAAGGAGAAGGAAAATGAGCTTGAAAATCTATTAGAGGATGATGAGATCTATTGGAAGCAGAGAACCCGGGAGGATTGGTTAACTTGGGTGACCGCAATACTAAATGGTTCCATCTGA

Coding sequence (CDS)

ATGATGGATCGAGGCCAGAAAGAAGGTCAAGTGCGTGAATCCATTACGTTAGTCGGTACTAAGCATAAGACAGAGGAGAGTGGGGGATTGATGTTACTATGGAGGAATGAGACTAAGGTTAGTATTCACTCATATTCGAAGGGGCATATAGATGCGACTATCCAGGAAGGAGGTTGGTCATGGCGGTTTACAGGCATTTATGGAAACCCAGTTAGAGGTTTGCATCATGAAACGTGGAGGTTAATGACTAGACTAGCTGAGCAGTCAGATATTCGTGGGTGCTGGGTGGTGACTTTAGTGAGATTACATATGGGTATGAGAAAAAGTGTATTCAAAGTACACCATTTGGCTCTGGTAGCAACTGACCATAGACCTCTCCTAGCGGAATGGAAAGAAGAACCACCTGATATAAATCATAATTGGAGTAGGCGACACTATGAAGGATCCATTAGAGGAGCTATTAATCATAAAGAAGTGGAAATTCAACACCTTCTCAGGCTTGGAGATGATCTTAGGGACAATGAGCTTAAGGAGAAGGAAAATGAGCTTGAAAATCTATTAGAGGATGATGAGATCTATTGGAAGCAGAGAACCCGGGAGGATTGGTTAACTTGGGTGACCGCAATACTAAATGGTTCCATCTGA

Protein sequence

MMDRGQKEGQVRESITLVGTKHKTEESGGLMLLWRNETKVSIHSYSKGHIDATIQEGGWSWRFTGIYGNPVRGLHHETWRLMTRLAEQSDIRGCWVVTLVRLHMGMRKSVFKVHHLALVATDHRPLLAEWKEEPPDINHNWSRRHYEGSIRGAINHKEVEIQHLLRLGDDLRDNELKEKENELENLLEDDEIYWKQRTREDWLTWVTAILNGSI
Homology
BLAST of Lag0017733 vs. NCBI nr
Match: XP_028113864.1 (uncharacterized protein LOC114311894 [Camellia sinensis])

HSP 1 Score: 90.9 bits (224), Expect = 1.4e-14
Identity = 62/204 (30.39%), Postives = 100/204 (49.02%), Query Frame = 0

Query: 20  TKHKTEESGGLMLLWRNETKVSIHSYSKGHIDATI--QEGGWSWRFTGIYGNPVRGLHHE 79
           T  + E SGGL LLW  E ++ I SYSKGH+D+ I  + G  SW+FTG YGNP   L  +
Sbjct: 15  TVDRVELSGGLALLWVGELQIQIKSYSKGHVDSIILTESGATSWQFTGFYGNPNTSLRGD 74

Query: 80  TWRLMTRLAEQSDIRGCWVVTLVRLHMGMRKSVFKVHHLALVATDHRPLLAEWKE----- 139
           +W L+ RL +Q ++   WV        G    +   H  + +A   +  +  +++     
Sbjct: 75  SWELLRRLQDQMNLP--WVCA------GDFNEILYAHEKSRMAARSQRQMDGFRQVLSDC 134

Query: 140 EPPDINHNWSRRHYE-----------GSIRGAINHKEVEIQHL--LRLGDDLRDNELKEK 199
           +  D++ +    H             G+++ ++N K   IQ L  L +  D    EL+  
Sbjct: 135 DLADLDFDGQLLHVASQLQAWNWWVLGNVQRSLNLKLAAIQRLQALPIPSDADLAELRHV 194

Query: 200 ENELENLLEDDEIYWKQRTREDWL 204
           + E++ LLE + + W QR R +WL
Sbjct: 195 QLEVDELLEKESVMWAQRARANWL 210

BLAST of Lag0017733 vs. NCBI nr
Match: XP_006487889.1 (uncharacterized protein LOC102617714 [Citrus sinensis])

HSP 1 Score: 90.1 bits (222), Expect = 2.5e-14
Identity = 66/208 (31.73%), Postives = 95/208 (45.67%), Query Frame = 0

Query: 28  GGLMLLWRNETKVSIHSYSKGHIDATI-QEGGWSWRFTGIYGNPVRGLHHETWRLMTRLA 87
           GGL LLW  +  + + SYSK HIDA I  E G SWR T +YG+P       TW L+ RLA
Sbjct: 66  GGLALLWTLDVSLEVKSYSKHHIDAVIHNENGSSWRCTCVYGHPESEQKKHTWSLLRRLA 125

Query: 88  EQSDIRG-CW-----VVTLVRLHMGMRKSVFKVHHLALVATDHRPLLAEWKEEPPDINHN 147
             S +   C+     +  L     G  ++  +VH       D R L    K  P      
Sbjct: 126 GMSSLPWLCFGDFNEIFNLNEKVGGNERNPSRVHEFRQAVRDCRLLDLGLKGYP----FT 185

Query: 148 WSRRHYEGSI-----------------RGAINHKEVEIQHLLRLGDDLR--------DNE 204
           WS R  E  I                 +     ++ +++ L      +R         +E
Sbjct: 186 WSNRRNEAKILFNKKARESLGELQLWSKKEFGGRQKQLEQLQNKLKSIRHSFSHYDCGDE 245

BLAST of Lag0017733 vs. NCBI nr
Match: XP_022157437.1 (uncharacterized protein LOC111024135 [Momordica charantia])

HSP 1 Score: 88.2 bits (217), Expect = 9.4e-14
Identity = 53/145 (36.55%), Postives = 71/145 (48.97%), Query Frame = 0

Query: 24  TEESGGLMLLWRNETKVSIHSYSKGHIDATIQEGGWSWRFTGIYGNPVRGLHHETWRLMT 83
           T +SGGLMLLW +++ V I S S GHID+ I +   SWRFTG YGNP       +W+L+ 
Sbjct: 62  TGKSGGLMLLWNSDSNVRIQSMSPGHIDSIITDKYGSWRFTGFYGNPCTYKRSASWKLLE 121

Query: 84  RLA----------------------------EQSDIRGCWVVT-----LVRLHMGMRKSV 136
           RLA                             +S +RGC +       L+   M  +   
Sbjct: 122 RLARMMDLPWIIGGDFNEIVSMTEKMGGVCRNESQMRGCPIWERLDRFLINESMLNKCLN 181

BLAST of Lag0017733 vs. NCBI nr
Match: KAF4364765.1 (hypothetical protein F8388_018441 [Cannabis sativa])

HSP 1 Score: 81.3 bits (199), Expect = 1.1e-11
Identity = 70/227 (30.84%), Postives = 102/227 (44.93%), Query Frame = 0

Query: 4   RGQKEGQVRESITLVGTKHKTEESGGLMLLWRNETKVSIHSYSKGHIDATIQ-EGGWSWR 63
           RG K G    S   +  K     SGGL+LLW ++ +VS+ S+S GHIDA +Q  G   WR
Sbjct: 199 RGSKGGSKLSSFP-ISPKLSHVMSGGLLLLWNDDWEVSVKSFSVGHIDALVQCPGRRLWR 258

Query: 64  FTGIYGNPVRGLHHETWRLMTRLAEQSDIRGCW--------VVTLVRLHMGMRKSVFKVH 123
           FTG YGNP      E+WRL+ RL +  D+   W        V+++     G  +S+  + 
Sbjct: 259 FTGFYGNPKASCRTESWRLLCRLKDLFDLP--WICGGHFNEVLSINEKKGGADRSLSAIS 318

Query: 124 HL-------ALVATDHRPLLAEWKEEPPDINHNWSRRHYEGSI-RGAINHK--------- 183
                    +L     +     W      IN      H +  + R   N +         
Sbjct: 319 DFQQALDTCSLADMGFQGQCFTW------INKRQGAAHVQERLDRFCCNQEWHNLFPSET 378

Query: 184 EVEIQHLLRLGDDL-RDNELKEKENELENLLEDDEIYWKQRTREDWL 204
           + ++  LL +   L R  E+K  E +L +LL  +E YWK R+R DWL
Sbjct: 379 QKQLDDLLSVAAPLVRMEEVKRLETKLNDLLSREECYWKLRSRADWL 416

BLAST of Lag0017733 vs. NCBI nr
Match: KAF4381998.1 (hypothetical protein G4B88_006630 [Cannabis sativa])

HSP 1 Score: 75.9 bits (185), Expect = 4.8e-10
Identity = 33/72 (45.83%), Postives = 48/72 (66.67%), Query Frame = 0

Query: 21  KHKTEESGGLMLLWRNETKVSIHSYSKGHIDATIQ-EGGWSWRFTGIYGNPVRGLHHETW 80
           ++  E SGGL+LLW ++ +VS+ S++ GHIDA ++  G   WRFTG YGNP      E+W
Sbjct: 501 EYNNESSGGLLLLWNDDWEVSVKSFTSGHIDALVKCPGRELWRFTGFYGNPKASCRSESW 560

Query: 81  RLMTRLAEQSDI 92
           RL+ RL +  D+
Sbjct: 561 RLLCRLKDLFDL 572

BLAST of Lag0017733 vs. ExPASy TrEMBL
Match: A0A6J1DUG8 (uncharacterized protein LOC111024135 OS=Momordica charantia OX=3673 GN=LOC111024135 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 4.5e-14
Identity = 53/145 (36.55%), Postives = 71/145 (48.97%), Query Frame = 0

Query: 24  TEESGGLMLLWRNETKVSIHSYSKGHIDATIQEGGWSWRFTGIYGNPVRGLHHETWRLMT 83
           T +SGGLMLLW +++ V I S S GHID+ I +   SWRFTG YGNP       +W+L+ 
Sbjct: 62  TGKSGGLMLLWNSDSNVRIQSMSPGHIDSIITDKYGSWRFTGFYGNPCTYKRSASWKLLE 121

Query: 84  RLA----------------------------EQSDIRGCWVVT-----LVRLHMGMRKSV 136
           RLA                             +S +RGC +       L+   M  +   
Sbjct: 122 RLARMMDLPWIIGGDFNEIVSMTEKMGGVCRNESQMRGCPIWERLDRFLINESMLNKCLN 181

BLAST of Lag0017733 vs. ExPASy TrEMBL
Match: A0A7J6F293 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_018441 PE=4 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 5.6e-12
Identity = 70/227 (30.84%), Postives = 102/227 (44.93%), Query Frame = 0

Query: 4   RGQKEGQVRESITLVGTKHKTEESGGLMLLWRNETKVSIHSYSKGHIDATIQ-EGGWSWR 63
           RG K G    S   +  K     SGGL+LLW ++ +VS+ S+S GHIDA +Q  G   WR
Sbjct: 199 RGSKGGSKLSSFP-ISPKLSHVMSGGLLLLWNDDWEVSVKSFSVGHIDALVQCPGRRLWR 258

Query: 64  FTGIYGNPVRGLHHETWRLMTRLAEQSDIRGCW--------VVTLVRLHMGMRKSVFKVH 123
           FTG YGNP      E+WRL+ RL +  D+   W        V+++     G  +S+  + 
Sbjct: 259 FTGFYGNPKASCRTESWRLLCRLKDLFDLP--WICGGHFNEVLSINEKKGGADRSLSAIS 318

Query: 124 HL-------ALVATDHRPLLAEWKEEPPDINHNWSRRHYEGSI-RGAINHK--------- 183
                    +L     +     W      IN      H +  + R   N +         
Sbjct: 319 DFQQALDTCSLADMGFQGQCFTW------INKRQGAAHVQERLDRFCCNQEWHNLFPSET 378

Query: 184 EVEIQHLLRLGDDL-RDNELKEKENELENLLEDDEIYWKQRTREDWL 204
           + ++  LL +   L R  E+K  E +L +LL  +E YWK R+R DWL
Sbjct: 379 QKQLDDLLSVAAPLVRMEEVKRLETKLNDLLSREECYWKLRSRADWL 416

BLAST of Lag0017733 vs. ExPASy TrEMBL
Match: A0A2N9ER53 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS5187 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 2.8e-11
Identity = 66/215 (30.70%), Postives = 99/215 (46.05%), Query Frame = 0

Query: 28   GGLMLLWRNETKVSIHSYSKGHIDA-TIQEGGWSWRFTGIYGNPVRGLHHETWRLM---- 87
            GGL LLW +   V I SYS  HIDA  + E G  WR TG YG+P RGL   +W L+    
Sbjct: 1082 GGLALLWNSSIAVHIQSYSHHHIDADVVFENGLRWRVTGFYGHPERGLRGHSWALLRQLW 1141

Query: 88   ----------------TRLAEQSDIRGCWVVTLVRLHMGMRK--SVF---KVHHLALVAT 147
                            T L EQ       +  +  L +   +  S+F   +VHH+ + A 
Sbjct: 1142 GIRNLPWLVLGDFNEITALNEQRGRIDRNLAQMAALCVANAEWISLFPNVRVHHVVVAAL 1201

Query: 148  DHRPLLAEWKEEPPDINHNWSRR---HYE-----GSIRGA---INHKEVEIQHLLRLG-D 205
            D   LL     +P    +N  ++   H+E      ++R     I  K+  ++ L  L  D
Sbjct: 1202 DQMGLLVSM--DPAQALNNCRKKKRFHFEHMWVHEAVRVTPCLIATKKARLEQLENLPMD 1261

BLAST of Lag0017733 vs. ExPASy TrEMBL
Match: A0A803NVA0 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 3.6e-11
Identity = 37/69 (53.62%), Postives = 45/69 (65.22%), Query Frame = 0

Query: 27  SGGLMLLWRNETKVSIHSYSKGHIDATIQEGGW-SWRFTGIYGNPVRGLHHETWRLMTRL 86
           SGGL LLW+ E +V+I  +S  HIDA IQ+ G  SWRFTGIYG P R L  +TW L   L
Sbjct: 405 SGGLALLWKEENEVTIQGFSANHIDAMIQKVGTPSWRFTGIYGEPRRELRFQTWNLFRSL 464

Query: 87  AEQSDIRGC 95
            E +D+  C
Sbjct: 465 KEDNDLPWC 473

BLAST of Lag0017733 vs. ExPASy TrEMBL
Match: A0A803P941 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.4e-10
Identity = 69/219 (31.51%), Postives = 105/219 (47.95%), Query Frame = 0

Query: 13  ESITLVGTKHKTEESGGLMLLWRNETKVSIHSYSKGHIDATIQEG-GWSWRFTGIYGNPV 72
           +S  +V  K K   SGGL LLW++  +V+I S++  HIDA ++ G G+SWRFTG YG+P 
Sbjct: 405 DSCFVVAAKGK---SGGLALLWKDSWEVNIKSFTVSHIDAAVENGLGFSWRFTGFYGSPD 464

Query: 73  RGLHHETWRLMTRLAEQSDIRGCWVVTLVRLHMGMRKSVFKVHHLALVATDHRPLLAEWK 132
            G    TW LM RL  ++ ++G W+       + + K           +  H       +
Sbjct: 465 PGGRKHTWLLMERL--RNMLQGPWLCGGDFNEIMVEKEKKWGASSKWKSRFHYEHAWASE 524

Query: 133 EEPPDINHN-WSRRHYEGSI---RGAIN-----------HKEVEI--------QHLLRLG 192
           EE   I  N W  R + GS+   RG IN            K+ E+        + L RL 
Sbjct: 525 EECGKIVENVWMDRGHWGSVQGLRGRINVCGEVLKEWNKGKKTELKAKTKALKEDLKRLS 584

Query: 193 DDLRDNELKEK---ENELENLLEDDEIYWKQRTREDWLT 205
             L + + +++   E +L    + +E+ WKQR+R  WLT
Sbjct: 585 SSLGEVDWQKRRQIERDLNAAEDKEELLWKQRSRALWLT 618

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_028113864.11.4e-1430.39uncharacterized protein LOC114311894 [Camellia sinensis][more]
XP_006487889.12.5e-1431.73uncharacterized protein LOC102617714 [Citrus sinensis][more]
XP_022157437.19.4e-1436.55uncharacterized protein LOC111024135 [Momordica charantia][more]
KAF4364765.11.1e-1130.84hypothetical protein F8388_018441 [Cannabis sativa][more]
KAF4381998.14.8e-1045.83hypothetical protein G4B88_006630 [Cannabis sativa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DUG84.5e-1436.55uncharacterized protein LOC111024135 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A7J6F2935.6e-1230.84Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_018441 PE=4 SV=1[more]
A0A2N9ER532.8e-1130.70Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS5187 PE=4 SV=1[more]
A0A803NVA03.6e-1153.62Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803P9411.4e-1031.51Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 169..189

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0017733.1Lag0017733.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005488 binding
molecular_function GO:0003824 catalytic activity