Lag0017649 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0017649
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionF-box protein SKIP23-like
Locationchr5: 6387210 .. 6388394 (-)
RNA-Seq ExpressionLag0017649
SyntenyLag0017649
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTTTCCGAGTACTTTGGAGACTGGGCAGCACTTGAATATGATGTTCTTGGTCTGATTCTCAACAAAACGGTGTCGCTCTACGATTATCTTCACTTCAGCCTCGTATGTAAGTCTTGGAATTTCATTGCATCACGCTACAAACACCAACCATCCATAATTACTTCCAAGTTTTCTCAACTTCCCATGCTCATTGTTCCCTCAAAACATGGTACCCAAAAACAACATTCTCTTTACCATCTCCCCACAAATCATATCCGCTCTGTTGACTTCAAATTTTGCTTCGACAAAAGGTGTTGTGGTTCGTCTTTTGGTTGGTTGATCATGCTCGAAGAGACACTTGATATTACTTTATTCAACCCTTTCTTAGGAAGTGTGATTCGTTTTCCTGCCATTGATTTATATCCTGATAATCCACTTGTTTATACTCCAAAGAGTATAGTTAGAGCTATAGTAACTAAAGATCCATCGTTATATCCAAATGATTACATGGTTGTAGTCATCTATAGTTCATTTCACCAATTGTGTCTCATGAAACCAAATGACAAAGCTTGGATTTACGAAAACTCAGAAGAGAAACGACATTATGTAGAAGATGTCAATGTTTGTGACAATACTTTATATGCTCTAGCCTATGGAGATAAAGGGTTGACACTTGGGAAAGTTGAGGTTGAGAATAATAGCGATGATGGAAGTTCCTCTATTATTGCTTTAAAGATGGTCACACCAGACTTTTTCGTGGATGATCCAAATGATCTAGCATATTTTGCTGAGTCTTCCAAAAAAGAGCTTTTATTGATCAGGAGGCTCTTTTCTATTGAGAAAGAGAGTGAACAAGGATACTCCAAGATTGCGTGTGTGAACACTATCAAATTCTTAGTCTACAAACTTACTCATGGAATGCAACGGTTTGTAGAGGTGGATAGTTTAGATGGTGATGCTATGTTTATTGGTGACAATCAGTCGACGTGCGTTTCAACTAAGGATTTTCCTCAATGTTTACCAAATCGTATATATTATACAGATAGTTCTCCTTACTTATGTCCACCTCGAGATGTAGGAATATACAACTTAGAAGATAAAAGCTTTGGTAAACATTACATAACAGATGATGCTCACAAAGATTTGCCTCCACCTATATGGATCGTCCCAACAATATGCTCCAAAGGTTTCAAGGAGTAG

mRNA sequence

ATGGATTTTTCCGAGTACTTTGGAGACTGGGCAGCACTTGAATATGATGTTCTTGGTCTGATTCTCAACAAAACGGTGTCGCTCTACGATTATCTTCACTTCAGCCTCGTATGTAAGTCTTGGAATTTCATTGCATCACGCTACAAACACCAACCATCCATAATTACTTCCAAGTTTTCTCAACTTCCCATGCTCATTGTTCCCTCAAAACATGGTACCCAAAAACAACATTCTCTTTACCATCTCCCCACAAATCATATCCGCTCTGTTGACTTCAAATTTTGCTTCGACAAAAGGTGTTGTGGTTCGTCTTTTGGTTGGTTGATCATGCTCGAAGAGACACTTGATATTACTTTATTCAACCCTTTCTTAGGAAGTGTGATTCGTTTTCCTGCCATTGATTTATATCCTGATAATCCACTTGTTTATACTCCAAAGAGTATAGTTAGAGCTATAGTAACTAAAGATCCATCGTTATATCCAAATGATTACATGGTTGTAGTCATCTATAGTTCATTTCACCAATTGTGTCTCATGAAACCAAATGACAAAGCTTGGATTTACGAAAACTCAGAAGAGAAACGACATTATGTAGAAGATGTCAATGTTTGTGACAATACTTTATATGCTCTAGCCTATGGAGATAAAGGGTTGACACTTGGGAAAGTTGAGGTTGAGAATAATAGCGATGATGGAAGTTCCTCTATTATTGCTTTAAAGATGGTCACACCAGACTTTTTCGTGGATGATCCAAATGATCTAGCATATTTTGCTGAGTCTTCCAAAAAAGAGCTTTTATTGATCAGGAGGCTCTTTTCTATTGAGAAAGAGAGTGAACAAGGATACTCCAAGATTGCGTGTGTGAACACTATCAAATTCTTAGTCTACAAACTTACTCATGGAATGCAACGGTTTGTAGAGGTGGATAGTTTAGATGGTGATGCTATGTTTATTGGTGACAATCAGTCGACGTGCGTTTCAACTAAGGATTTTCCTCAATGTTTACCAAATCGTATATATTATACAGATAGTTCTCCTTACTTATGTCCACCTCGAGATGTAGGAATATACAACTTAGAAGATAAAAGCTTTGGTAAACATTACATAACAGATGATGCTCACAAAGATTTGCCTCCACCTATATGGATCGTCCCAACAATATGCTCCAAAGGTTTCAAGGAGTAG

Coding sequence (CDS)

ATGGATTTTTCCGAGTACTTTGGAGACTGGGCAGCACTTGAATATGATGTTCTTGGTCTGATTCTCAACAAAACGGTGTCGCTCTACGATTATCTTCACTTCAGCCTCGTATGTAAGTCTTGGAATTTCATTGCATCACGCTACAAACACCAACCATCCATAATTACTTCCAAGTTTTCTCAACTTCCCATGCTCATTGTTCCCTCAAAACATGGTACCCAAAAACAACATTCTCTTTACCATCTCCCCACAAATCATATCCGCTCTGTTGACTTCAAATTTTGCTTCGACAAAAGGTGTTGTGGTTCGTCTTTTGGTTGGTTGATCATGCTCGAAGAGACACTTGATATTACTTTATTCAACCCTTTCTTAGGAAGTGTGATTCGTTTTCCTGCCATTGATTTATATCCTGATAATCCACTTGTTTATACTCCAAAGAGTATAGTTAGAGCTATAGTAACTAAAGATCCATCGTTATATCCAAATGATTACATGGTTGTAGTCATCTATAGTTCATTTCACCAATTGTGTCTCATGAAACCAAATGACAAAGCTTGGATTTACGAAAACTCAGAAGAGAAACGACATTATGTAGAAGATGTCAATGTTTGTGACAATACTTTATATGCTCTAGCCTATGGAGATAAAGGGTTGACACTTGGGAAAGTTGAGGTTGAGAATAATAGCGATGATGGAAGTTCCTCTATTATTGCTTTAAAGATGGTCACACCAGACTTTTTCGTGGATGATCCAAATGATCTAGCATATTTTGCTGAGTCTTCCAAAAAAGAGCTTTTATTGATCAGGAGGCTCTTTTCTATTGAGAAAGAGAGTGAACAAGGATACTCCAAGATTGCGTGTGTGAACACTATCAAATTCTTAGTCTACAAACTTACTCATGGAATGCAACGGTTTGTAGAGGTGGATAGTTTAGATGGTGATGCTATGTTTATTGGTGACAATCAGTCGACGTGCGTTTCAACTAAGGATTTTCCTCAATGTTTACCAAATCGTATATATTATACAGATAGTTCTCCTTACTTATGTCCACCTCGAGATGTAGGAATATACAACTTAGAAGATAAAAGCTTTGGTAAACATTACATAACAGATGATGCTCACAAAGATTTGCCTCCACCTATATGGATCGTCCCAACAATATGCTCCAAAGGTTTCAAGGAGTAG

Protein sequence

MDFSEYFGDWAALEYDVLGLILNKTVSLYDYLHFSLVCKSWNFIASRYKHQPSIITSKFSQLPMLIVPSKHGTQKQHSLYHLPTNHIRSVDFKFCFDKRCCGSSFGWLIMLEETLDITLFNPFLGSVIRFPAIDLYPDNPLVYTPKSIVRAIVTKDPSLYPNDYMVVVIYSSFHQLCLMKPNDKAWIYENSEEKRHYVEDVNVCDNTLYALAYGDKGLTLGKVEVENNSDDGSSSIIALKMVTPDFFVDDPNDLAYFAESSKKELLLIRRLFSIEKESEQGYSKIACVNTIKFLVYKLTHGMQRFVEVDSLDGDAMFIGDNQSTCVSTKDFPQCLPNRIYYTDSSPYLCPPRDVGIYNLEDKSFGKHYITDDAHKDLPPPIWIVPTICSKGFKE
Homology
BLAST of Lag0017649 vs. NCBI nr
Match: XP_022923266.1 (probable F-box protein At1g65740 [Cucurbita moschata])

HSP 1 Score: 434.9 bits (1117), Expect = 7.5e-118
Identity = 233/398 (58.54%), Postives = 280/398 (70.35%), Query Frame = 0

Query: 7   FGDWAALEYDVLGLILNKTVSLYDYLHFSLVCKSWNFIASRYKHQPSIITSKFSQLPMLI 66
           F DW AL+YDVLG+ILNK VSLYDYL FS+VCKSW F+A R+KHQ SIITSKF QLPMLI
Sbjct: 6   FRDWTALDYDVLGVILNKMVSLYDYLQFSIVCKSWYFVALRHKHQRSIITSKFPQLPMLI 65

Query: 67  VPSKHGTQKQHSLYHLPTNHIRSVDFKFCFDKRCCGSSFGWLIMLEETLDITLFNPFLGS 126
           VPSK+G +KQH LY    N  R VDFKFCF+KRCCGSSFGWLIM EETL++TLFNPF G+
Sbjct: 66  VPSKNGLEKQHCLYDPRKNRTRPVDFKFCFNKRCCGSSFGWLIMCEETLNLTLFNPFSGT 125

Query: 127 VIRFPAIDLYPDNPLVYTPKSIVRAIVTKDPSLYPNDYMVVVIYSSFHQLCLMKPNDKAW 186
           VI  P+I  + D+   Y P  IV+AI+TKDPSLYPNDYMVV IYSS+ +LCLM+ NDK+W
Sbjct: 126 VICLPSI--FIDDEPDYAPDRIVKAILTKDPSLYPNDYMVVAIYSSYSRLCLMRANDKSW 185

Query: 187 IYENSEEKRHYVEDVNVCDNTLYALAYGDKGLTLGKVEVENNSDDGSSSIIALKMV---- 246
            Y   E    + +D+NV  +TLYA    +  + + KVEV+N  D+ SS  I+LKMV    
Sbjct: 186 TYSYPEPNIPF-KDINVSSDTLYAT---ENNMMVWKVEVKNRDDESSSISISLKMVHAKR 245

Query: 247 TPDFFVDDPNDLAYFAESSKKELLLIRRLFSIEKESEQGYSKIACVNTIKFLVYKL---- 306
           T DF V      +Y  ESSK ELLL+ R  SI +E  Q       + T KF VYKL    
Sbjct: 246 TNDFVV------SYITESSKGELLLVLR--SISEEDSQ-------LETTKFKVYKLIDEC 305

Query: 307 THGMQRFVEVDSLDGDAMFIGDNQSTCVSTKDFPQCLPNRIYYTDSSPYLCP--PRDVGI 366
            + M R V++ SLDGDAMFIGD+QS CVSTK FP+CLPN IY+ +      P   +D GI
Sbjct: 306 ENDMLRAVKLKSLDGDAMFIGDSQSICVSTKYFPKCLPNCIYFINGYYNYIPNLEQDSGI 365

Query: 367 YNLEDKSFGKHYITDDAHKDLPPPIWIVPTICSKGFKE 395
           +NLEDK F  HYI D AHK+LP PIWI+PTICSK F++
Sbjct: 366 FNLEDKRFANHYIRDPAHKNLPQPIWIIPTICSKDFED 382

BLAST of Lag0017649 vs. NCBI nr
Match: XP_023552957.1 (probable F-box protein At1g65740 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 432.2 bits (1110), Expect = 4.9e-117
Identity = 234/398 (58.79%), Postives = 277/398 (69.60%), Query Frame = 0

Query: 7   FGDWAALEYDVLGLILNKTVSLYDYLHFSLVCKSWNFIASRYKHQPSIITSKFSQLPMLI 66
           F DW AL+YDVLG+ILNK VSLYDYL FS+VCKSW F+A R+KHQ SIITSKF QLPMLI
Sbjct: 6   FRDWTALDYDVLGVILNKMVSLYDYLQFSIVCKSWYFVALRHKHQRSIITSKFPQLPMLI 65

Query: 67  VPSKHGTQKQHSLYHLPTNHIRSVDFKFCFDKRCCGSSFGWLIMLEETLDITLFNPFLGS 126
           VPSK G +KQH LY    N  R VDFKFCF+KRCCGSSFGWLIM EETLD+TLFNPF G+
Sbjct: 66  VPSKDGLEKQHCLYDPRKNRTRPVDFKFCFNKRCCGSSFGWLIMCEETLDLTLFNPFSGT 125

Query: 127 VIRFPAIDLYPDNPLVYTPKSIVRAIVTKDPSLYPNDYMVVVIYSSFHQLCLMKPNDKAW 186
           VI  P+I    D P  Y P  IV+AI+TKDP LYPNDYMVV IYSS+ +LCLM+ NDK+W
Sbjct: 126 VICLPSI-FIEDEP-DYAPDRIVKAILTKDPWLYPNDYMVVAIYSSYSRLCLMRANDKSW 185

Query: 187 IYENSEEKRHYVEDVNVCDNTLYALAYGDKGLTLGKVEVENNSDDGSSSIIALKMV---- 246
            Y   E    + +D+NV  +TLYA    +  + + KVEV+N  D+ SS  I+LKMV    
Sbjct: 186 TYSYPEPNIPF-KDINVSSDTLYAT---ENNMMVWKVEVKNRDDESSSISISLKMVHAKR 245

Query: 247 TPDFFVDDPNDLAYFAESSKKELLLIRRLFSIEKESEQGYSKIACVNTIKFLVYKL---- 306
           T DF V      +Y  ESSK ELLL+ R  SI +E  Q       + T KF VYKL    
Sbjct: 246 TNDFVV------SYITESSKGELLLVLR--SISEEDSQ-------LETTKFKVYKLIDEC 305

Query: 307 THGMQRFVEVDSLDGDAMFIGDNQSTCVSTKDFPQCLPNRIYYTDSSPYLCP--PRDVGI 366
            + M R V++ SLDGDAMFIGD+QS CVSTK FP+CLPN IY+ +      P   +D GI
Sbjct: 306 ENDMLRAVKLKSLDGDAMFIGDSQSICVSTKYFPKCLPNCIYFINGYYNYIPNLEQDSGI 365

Query: 367 YNLEDKSFGKHYITDDAHKDLPPPIWIVPTICSKGFKE 395
           +NLEDK F  HYI D AHK+LP PIWI+PTICSK F++
Sbjct: 366 FNLEDKRFANHYIRDPAHKNLPQPIWIIPTICSKDFED 382

BLAST of Lag0017649 vs. NCBI nr
Match: KAG6577578.1 (putative F-box protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 432.2 bits (1110), Expect = 4.9e-117
Identity = 232/398 (58.29%), Postives = 278/398 (69.85%), Query Frame = 0

Query: 7   FGDWAALEYDVLGLILNKTVSLYDYLHFSLVCKSWNFIASRYKHQPSIITSKFSQLPMLI 66
           F DW AL+YDVLG+ILNK VSLYDYL FS+VCKSW F+A R+KHQ SIITSKF QLPMLI
Sbjct: 6   FRDWTALDYDVLGVILNKMVSLYDYLQFSIVCKSWYFVALRHKHQRSIITSKFPQLPMLI 65

Query: 67  VPSKHGTQKQHSLYHLPTNHIRSVDFKFCFDKRCCGSSFGWLIMLEETLDITLFNPFLGS 126
           VPSK G + QH LY    N  R VDFKFCF+KRCCGSSFGWLIM EETL++TLFNPF G+
Sbjct: 66  VPSKDGLENQHCLYDPRKNRTRPVDFKFCFNKRCCGSSFGWLIMCEETLNLTLFNPFSGT 125

Query: 127 VIRFPAIDLYPDNPLVYTPKSIVRAIVTKDPSLYPNDYMVVVIYSSFHQLCLMKPNDKAW 186
           VI  P+I  + D+   Y P  IV+AI+TKDPSLYPNDYMVV IYSS+ +LCLM+ NDK+W
Sbjct: 126 VICLPSI--FIDDEPDYAPDRIVKAILTKDPSLYPNDYMVVAIYSSYSRLCLMRANDKSW 185

Query: 187 IYENSEEKRHYVEDVNVCDNTLYALAYGDKGLTLGKVEVENNSDDGSSSIIALKMV---- 246
            Y   E    + +D+NV  +TLYA    +  + + KVEV+N  D+ SS  I+LKMV    
Sbjct: 186 TYSYPEPNIPF-KDINVSSDTLYAT---ENNMMVWKVEVKNRDDESSSISISLKMVHAKR 245

Query: 247 TPDFFVDDPNDLAYFAESSKKELLLIRRLFSIEKESEQGYSKIACVNTIKFLVYKL---- 306
           T DF V      +Y  ESSK ELLL+ R  SI +E  Q       + T KF VYKL    
Sbjct: 246 TNDFVV------SYITESSKGELLLVLR--SISEEDSQ-------LETTKFKVYKLIDEC 305

Query: 307 THGMQRFVEVDSLDGDAMFIGDNQSTCVSTKDFPQCLPNRIYYTDSSPYLCP--PRDVGI 366
            + M R V++ SLDGDAMFIGD+QS CVSTK FP+CLPN IY+ +      P   +D GI
Sbjct: 306 ENDMLRAVKLKSLDGDAMFIGDSQSICVSTKYFPKCLPNCIYFINGYYNYIPNLEQDSGI 365

Query: 367 YNLEDKSFGKHYITDDAHKDLPPPIWIVPTICSKGFKE 395
           +NLEDK F  HYI D AHK+LP PIWI+PTICSK F++
Sbjct: 366 FNLEDKRFANHYIRDPAHKNLPQPIWIIPTICSKDFED 382

BLAST of Lag0017649 vs. NCBI nr
Match: KAA0046890.1 (F-box protein SKIP23-like [Cucumis melo var. makuwa])

HSP 1 Score: 427.6 bits (1098), Expect = 1.2e-115
Identity = 220/395 (55.70%), Postives = 275/395 (69.62%), Query Frame = 0

Query: 9   DWAALEYDVLGLILNKTVSLYDYLHFSLVCKSWNFIASRYKHQPSIITSKFSQLPMLIVP 68
           DW+ LEYDVLG+ILNK VSLYDYL FS VCKSWNFIA R+KHQ S+ITS  SQLPMLIVP
Sbjct: 8   DWSTLEYDVLGVILNKMVSLYDYLQFSRVCKSWNFIALRHKHQRSLITSNHSQLPMLIVP 67

Query: 69  SKHGTQKQHSLYHLPTNHIRSVDFKFCFDKRCCGSSFGWLIMLEETLDITLFNPFLGSVI 128
           S++ ++KQH LY L  N IR VDF   F+KRCCGSSFGWLI+LEETLDITLFNPF G+ I
Sbjct: 68  SEYDSEKQHCLYDLTNNEIRPVDFVCSFNKRCCGSSFGWLILLEETLDITLFNPFNGNKI 127

Query: 129 RFPAIDLYPDNPLVYTPKSIVRAIVTKDPSLYPNDYMVVVIYSSFHQLCLMKPNDKAWIY 188
             P I +  D+   Y P +I +AI+TKDPSLYP+ + +V IYSS+ +LCLM+  DK WIY
Sbjct: 128 HIPPITI--DDEPDYCPLAIHKAILTKDPSLYPHGFTIVAIYSSYRRLCLMEAKDKRWIY 187

Query: 189 ENSEEKRHYVEDVNVCDNTLYALAYGDKGLTLGKVEVENNSDDGSSSIIALKMVTPDFFV 248
            N      + +D+NV D+ LY L +   GL + KVE        +SSI   K++    FV
Sbjct: 188 HNLNRTNGF-DDINVLDDVLYVLDW-RIGLWIAKVE--------NSSIYLKKVIFSIDFV 247

Query: 249 DDPNDLAYFAESSKKELLLIRRLFSIEKESEQGYSKIACVNTIKFLVYKLTH----GMQR 308
           +   D+AY   SSK+ELLL+ R    E+E +     +    T KF+VYK+T     G   
Sbjct: 248 E--ADIAYLVVSSKRELLLVSRFVIDEREGQDPDGIMLFTRTSKFIVYKVTRECEDGTMS 307

Query: 309 FVEVDSLDGDAMFIGDNQSTCVSTKDFPQCLPNRIYYTDSS-----PYLCPPRDVGIYNL 368
            VEV+SLDGDAMFIGDNQS C ST+DFP+CLP+R+Y+TD+      PYL   +D GIY L
Sbjct: 308 VVEVESLDGDAMFIGDNQSICASTRDFPKCLPDRVYFTDNCHHYRFPYLHGHQDAGIYRL 367

Query: 369 EDKSFGKHYITDDAHKDLPPPIWIVPTICSKGFKE 395
           EDKSF +HYI D AH++LPPPIWI+PTIC + F++
Sbjct: 368 EDKSFDRHYIVDSAHRNLPPPIWIIPTICYRDFED 388

BLAST of Lag0017649 vs. NCBI nr
Match: KGN56200.1 (hypothetical protein Csa_011053 [Cucumis sativus])

HSP 1 Score: 419.1 bits (1076), Expect = 4.3e-113
Identity = 221/395 (55.95%), Postives = 274/395 (69.37%), Query Frame = 0

Query: 9   DWAALEYDVLGLILNKTVSLYDYLHFSLVCKSWNFIASRYKHQPSIITSKFSQLPMLIVP 68
           DW+ LEYDVLG+ILNK VSLYDYL FS VCKSWNFIA RYKH  S+ITS   QLPMLIVP
Sbjct: 8   DWSTLEYDVLGMILNKMVSLYDYLQFSSVCKSWNFIALRYKHHRSLITSNLPQLPMLIVP 67

Query: 69  SKHGTQKQHSLYHLPTNHIRSVDFKFCFDKRCCGSSFGWLIMLEETLDITLFNPFLGSVI 128
           S++ + KQH LY L  N IR VDF   F+KRCCGSSFGWLIMLE+TLDITLFNPF G+VI
Sbjct: 68  SEYDSGKQHCLYDLINNEIRPVDFVCSFNKRCCGSSFGWLIMLEDTLDITLFNPFNGNVI 127

Query: 129 RFPAIDLYPDNPLVYTPKSIVRAIVTKDPSLYPNDYMVVVIYSSFHQLCLMKPNDKAWIY 188
             P I ++ D P  Y+P +I +AI+TKDPS+YP  + +V IYSSF +LCLM+  DK WIY
Sbjct: 128 HIPPITIH-DEP-GYSPIAIHKAILTKDPSIYPQGFTIVAIYSSFRRLCLMEAKDKRWIY 187

Query: 189 ENSEEKRHYVEDVNVCDNTLYALAYGDKGLTLGKVEVENNSDDGSSSIIALKMVTPDFFV 248
            N      + +DVNV D+ LY L   D  + L  V+VE++S       I LK V   F  
Sbjct: 188 HNLNRNNGF-DDVNVVDDILYVL---DWRIGLWIVKVEDSS-------IYLKKVIFSFNF 247

Query: 249 DDPNDLAYFAESSKKELLLIRRLFSIEKESEQGYSKIACVNTIKFLVYKLTH----GMQR 308
           ++  D+AY   SSK+ELLL+ R    + E +     +    T KF+V+K+TH    G+ R
Sbjct: 248 NEA-DIAYLIVSSKRELLLVSRFVVTQWEGQVPNEILKFTRTSKFIVHKVTHECEDGIMR 307

Query: 309 FVEVDSLDGDAMFIGDNQSTCVSTKDFPQCLPNRIYYTDSS-----PYLCPPRDVGIYNL 368
            VEV+SLDGD MFIGDNQS CVSTKDFP+CLP+RIY+TD+      PYL   +D GIY +
Sbjct: 308 VVEVESLDGDVMFIGDNQSICVSTKDFPKCLPDRIYFTDNCHHFAFPYLHGHQDAGIYRV 367

Query: 369 EDKSFGKHYITDDAHKDLPPPIWIVPTICSKGFKE 395
           EDKSF +HYI + AH++LPPPIWI+PTI  K  ++
Sbjct: 368 EDKSFDRHYIVNRAHRNLPPPIWIIPTISYKHLED 388

BLAST of Lag0017649 vs. ExPASy Swiss-Prot
Match: Q3EBZ2 (F-box protein SKIP23 OS=Arabidopsis thaliana OX=3702 GN=SKIP23 PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 4.1e-10
Identity = 99/417 (23.74%), Postives = 166/417 (39.81%), Query Frame = 0

Query: 9   DWAALEYDVLGLILNKTVSLYDYLHFSLVCKSWNFIASRYKHQPSIITSKFSQLPMLIVP 68
           DW+ L  D+L LI     S +D + F  VC SW   A      P+        LP  I+P
Sbjct: 3   DWSTLPKDLLDLISKSLESSFDLIQFRSVCSSWRSAAEPKSPLPT------HHLP--ILP 62

Query: 69  SKHGTQKQHSLYHLPTNHIRSVDFKFCFDKRCCGSSFGWLIMLEETLD----ITLFNPFL 128
              G+    S      +  RS+      +      SFGWLI +EE L+    +TL +P  
Sbjct: 63  DNGGSLFPDSAVGFRLSQ-RSILLIKPHEPCIESDSFGWLIKVEEDLNVPRKVTLLDPLC 122

Query: 129 GS-------------VIRFPAIDLYPDNPLVY--TPKSIVRAIVTKDPSL----YPNDYM 188
            +             + +F   +L  +  L Y  T   IV ++  +   +       DY 
Sbjct: 123 DTRNSIPENFPRVLDMSKFKVRELGREFKLHYFNTVGDIVESLYLEKAVVKYLDCDGDYK 182

Query: 189 VVVIYSSFH---QLCLMKPNDKAWIYENSEEKRHYVEDVNVCDNTLYALAYGDKGLTLGK 248
            V++  + H   +L + +  D+AW   N     +  +DV + D   +A+   + G T   
Sbjct: 183 FVLL--TIHVSGKLAVFRSWDRAWTVINDMPSPY--DDVMLFDGRFFAV--DNNGRT--- 242

Query: 249 VEVENNSDDGSSSIIALKMVTPDFFVDDPNDLAYFAESSKKELLLIRRLFSIEK-ESEQG 308
           V V+ +S       + L +V    F  D      F   S  E+LL+    S+E  E + G
Sbjct: 243 VVVDYSS-------LKLTLVASPVFGGDKK----FLIESCGEMLLVDMYLSLEAVEGDPG 302

Query: 309 YSK--------IACVNTIKFLVYKLTHGMQRFVEVDSLDGDAMFIGDNQSTCVSTKD-FP 368
           + +             T+KF VY+     + +V+V  L+   +F+GD+ +   S  D  P
Sbjct: 303 FVEEIFEHPAFYMNERTVKFKVYRFVEREESWVDVYDLEDKMLFLGDDSTFSASASDILP 362

Query: 369 QCLPNRIYYTDS-----SPYLCPPRDVGIYNLED-KSFGKHYITDDAHKDLPPPIWI 384
            C  + +++  +            RD+G+++    K      + + A    PPP WI
Sbjct: 363 LCDGSSVFFNGNVFNGEDLGAMQDRDLGVFDFRSGKIELVQKLPEYAKLFWPPPPWI 390

BLAST of Lag0017649 vs. ExPASy Swiss-Prot
Match: Q6DR20 (F-box protein At2g17036 OS=Arabidopsis thaliana OX=3702 GN=At2g17036 PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 5.1e-08
Identity = 89/414 (21.50%), Postives = 157/414 (37.92%), Query Frame = 0

Query: 9   DWAALEYDVLGLILNKTVSLYDYLHFSLVCKSWNFIASRYK-----HQPSIITSKFSQLP 68
           DWA L  D+L LI     S +D + F  VC SW   A   +     + P   +     L 
Sbjct: 3   DWATLPKDLLDLISKCLESSFDLIQFRSVCSSWRSAAGPKRLLWAHNLPFFPSDDKPFLS 62

Query: 69  MLIVPSKHGTQKQHSLYHLPTNHIRSVDFKFCFDKRCCGSSFGWLIMLEETL----DITL 128
            +I+   H      S+  +  N           + +C    FGW++ + + +     +TL
Sbjct: 63  NVILRVAH-----QSILLIKPN-----------EPQCEADLFGWIVKVWDNIYVSRKMTL 122

Query: 129 FNPFLGSVIRFPAIDLYPDNPLVYTPKSIVRAIVTKDPSLY------------------- 188
             P   S   FP       +   +T + + R +    P  Y                   
Sbjct: 123 LKPLSSSRNYFPQHLPRIFDMSKFTVRELCREVKLYHPDYYCVPGHTALELELGKTVVKY 182

Query: 189 -PNDYMVVVIYSSFHQLCLMKPNDKAWIYENSEEKRHYVEDVNVCDNTLYALAYGDKGLT 248
             +D  V++    + +L + +  D+ W   N        +D+ + D   +A+ Y  + + 
Sbjct: 183 LNDDKFVLLTILEYGKLAVFRSWDREWTVINDYIPSR-CQDLIMFDGRFFAIDYNGRTVV 242

Query: 249 LGKVEVENNSDDGSSSIIALKMVTPDFFVDDPNDLAYFAESSKKELLLIRRLFSIEKESE 308
           +          D SS  + L    P     D      F   S  E+ L+   F + ++ E
Sbjct: 243 V----------DYSSFKLTL-AANPLIGGGDKK----FLIESCGEMFLVDIEFCLNEKPE 302

Query: 309 --QGYSKIACVNTI--KFLVYKLTHGMQRFVEVDSLDGDAMFIGDNQSTCVSTKD-FPQC 368
              G+       T+  KF  +KL    +R+VEV+ L     F+GD+ +   ST D  P+C
Sbjct: 303 FTGGFYSYFNETTVSYKFKFFKLVEREKRWVEVEDLGDKMFFLGDDSTFSASTADIIPRC 362

Query: 369 LPNR---IYYT-DSSPYLCPPRDVGIYNLED-KSFGKHYITDDAHKDLPPPIWI 384
           +       +YT + S  +   R++G+++    K+   + + + A    PPP WI
Sbjct: 363 VGTGSFVFFYTHEESLVVMDDRNLGVFDFRSGKTELVNKLPEYAKLFWPPPPWI 384

BLAST of Lag0017649 vs. ExPASy Swiss-Prot
Match: Q1PEZ8 (F-box protein At2g26160 OS=Arabidopsis thaliana OX=3702 GN=At2g26160 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.6e-06
Identity = 73/356 (20.51%), Postives = 142/356 (39.89%), Query Frame = 0

Query: 9   DWAALEYDVLGLILNKTVSLYDYLHFSLVCKSWNFIASRYKHQPSIITSKFSQLPMLIVP 68
           +W+ L  D++ L  N+  S+ D L    +CK W   A+  K     + S    +  ++  
Sbjct: 5   EWSELPGDLINLTANRFSSISDVLRVRSICKPWRSAAATPKSFQCNLPSSNKMIETVL-- 64

Query: 69  SKHGTQKQHSLYHLPTNHIRSVDFKFCFDKRCCGSSFGWLI---MLEETLDITLFNPFLG 128
                         PT   R      C  K       GWLI    + E+  I L +PF  
Sbjct: 65  -------------SPTTFFRVTGPSSCSYK-------GWLIRTKQVSESSKINLLSPFFR 124

Query: 129 ----------SVIRFPAIDLYPDNPLVYTPKSIVRAIVTKDPSLYPNDYMVVVIYSSFHQ 188
                      +++F   ++     +    K +++ ++ K+    P+  +  V++   + 
Sbjct: 125 QLLTPSQQTLDLLKFEVSEIRQSYEIHIFDKYLIQGVIGKEG---PSHILSRVVFLD-NL 184

Query: 189 LCLMKPNDKAWIYENSEE-KRHYVEDVNVCDNTLYALAYGDKGLTLGKVEVENNSDDGSS 248
           +  +  +DK W  ++ EE  R + +  N  ++ L  + +  KG     +++       S 
Sbjct: 185 IFAVGQDDKIWCCKSGEESSRIWTKIKNQVEDFLDIILH--KGQVYA-LDLTGAIWWISL 244

Query: 249 SIIALKMVTPDFFVDDPNDLAYFAESSKK------ELLLIRRLFSIEKESEQGYSKIACV 308
           S ++L   TP      P D   +   +K+      +L +I +L       ++ Y +    
Sbjct: 245 SPLSLLQFTPSI----PMDYDGYDSCNKRLVEYCGDLCIIHQL-----RLKKAYIR-RSQ 304

Query: 309 NTIKFLVYKLTHGMQRFVEVDSLDGDAMFIGDNQSTCVSTKDFPQCLPNRIYYTDS 345
            T+ F VYK+   + ++VEV SL   A+ +  +    V   ++  CL N IY+ D+
Sbjct: 305 RTVGFKVYKMDEYVAKWVEVRSLGDKALIVARDSCFTVVASEYHGCLNNSIYFVDN 321

BLAST of Lag0017649 vs. ExPASy Swiss-Prot
Match: Q3EBY8 (F-box protein At2g17690 OS=Arabidopsis thaliana OX=3702 GN=SDC PE=2 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 8.9e-05
Identity = 28/74 (37.84%), Postives = 39/74 (52.70%), Query Frame = 0

Query: 290 TIKFLVYKLTHGMQRFVEVDSLDGDAMFIGDNQSTCVSTKDFPQCLPNRIYYTDSSPYLC 349
           TI F VYK    + ++VEV SL   A+ I  +    VS  +F  CLPN IY+TD      
Sbjct: 333 TIGFKVYKNDEELLKWVEVKSLGDKAIVIATDACFSVSAHEFYGCLPNSIYFTDKK---- 392

Query: 350 PPRDVGIYNLEDKS 364
              +V ++ L+D S
Sbjct: 393 -EEEVKVFKLDDGS 401

BLAST of Lag0017649 vs. ExPASy TrEMBL
Match: A0A6J1E6C7 (probable F-box protein At1g65740 OS=Cucurbita moschata OX=3662 GN=LOC111431011 PE=4 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 3.6e-118
Identity = 233/398 (58.54%), Postives = 280/398 (70.35%), Query Frame = 0

Query: 7   FGDWAALEYDVLGLILNKTVSLYDYLHFSLVCKSWNFIASRYKHQPSIITSKFSQLPMLI 66
           F DW AL+YDVLG+ILNK VSLYDYL FS+VCKSW F+A R+KHQ SIITSKF QLPMLI
Sbjct: 6   FRDWTALDYDVLGVILNKMVSLYDYLQFSIVCKSWYFVALRHKHQRSIITSKFPQLPMLI 65

Query: 67  VPSKHGTQKQHSLYHLPTNHIRSVDFKFCFDKRCCGSSFGWLIMLEETLDITLFNPFLGS 126
           VPSK+G +KQH LY    N  R VDFKFCF+KRCCGSSFGWLIM EETL++TLFNPF G+
Sbjct: 66  VPSKNGLEKQHCLYDPRKNRTRPVDFKFCFNKRCCGSSFGWLIMCEETLNLTLFNPFSGT 125

Query: 127 VIRFPAIDLYPDNPLVYTPKSIVRAIVTKDPSLYPNDYMVVVIYSSFHQLCLMKPNDKAW 186
           VI  P+I  + D+   Y P  IV+AI+TKDPSLYPNDYMVV IYSS+ +LCLM+ NDK+W
Sbjct: 126 VICLPSI--FIDDEPDYAPDRIVKAILTKDPSLYPNDYMVVAIYSSYSRLCLMRANDKSW 185

Query: 187 IYENSEEKRHYVEDVNVCDNTLYALAYGDKGLTLGKVEVENNSDDGSSSIIALKMV---- 246
            Y   E    + +D+NV  +TLYA    +  + + KVEV+N  D+ SS  I+LKMV    
Sbjct: 186 TYSYPEPNIPF-KDINVSSDTLYAT---ENNMMVWKVEVKNRDDESSSISISLKMVHAKR 245

Query: 247 TPDFFVDDPNDLAYFAESSKKELLLIRRLFSIEKESEQGYSKIACVNTIKFLVYKL---- 306
           T DF V      +Y  ESSK ELLL+ R  SI +E  Q       + T KF VYKL    
Sbjct: 246 TNDFVV------SYITESSKGELLLVLR--SISEEDSQ-------LETTKFKVYKLIDEC 305

Query: 307 THGMQRFVEVDSLDGDAMFIGDNQSTCVSTKDFPQCLPNRIYYTDSSPYLCP--PRDVGI 366
            + M R V++ SLDGDAMFIGD+QS CVSTK FP+CLPN IY+ +      P   +D GI
Sbjct: 306 ENDMLRAVKLKSLDGDAMFIGDSQSICVSTKYFPKCLPNCIYFINGYYNYIPNLEQDSGI 365

Query: 367 YNLEDKSFGKHYITDDAHKDLPPPIWIVPTICSKGFKE 395
           +NLEDK F  HYI D AHK+LP PIWI+PTICSK F++
Sbjct: 366 FNLEDKRFANHYIRDPAHKNLPQPIWIIPTICSKDFED 382

BLAST of Lag0017649 vs. ExPASy TrEMBL
Match: A0A5A7TUR9 (F-box protein SKIP23-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold230G00500 PE=4 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 5.8e-116
Identity = 220/395 (55.70%), Postives = 275/395 (69.62%), Query Frame = 0

Query: 9   DWAALEYDVLGLILNKTVSLYDYLHFSLVCKSWNFIASRYKHQPSIITSKFSQLPMLIVP 68
           DW+ LEYDVLG+ILNK VSLYDYL FS VCKSWNFIA R+KHQ S+ITS  SQLPMLIVP
Sbjct: 8   DWSTLEYDVLGVILNKMVSLYDYLQFSRVCKSWNFIALRHKHQRSLITSNHSQLPMLIVP 67

Query: 69  SKHGTQKQHSLYHLPTNHIRSVDFKFCFDKRCCGSSFGWLIMLEETLDITLFNPFLGSVI 128
           S++ ++KQH LY L  N IR VDF   F+KRCCGSSFGWLI+LEETLDITLFNPF G+ I
Sbjct: 68  SEYDSEKQHCLYDLTNNEIRPVDFVCSFNKRCCGSSFGWLILLEETLDITLFNPFNGNKI 127

Query: 129 RFPAIDLYPDNPLVYTPKSIVRAIVTKDPSLYPNDYMVVVIYSSFHQLCLMKPNDKAWIY 188
             P I +  D+   Y P +I +AI+TKDPSLYP+ + +V IYSS+ +LCLM+  DK WIY
Sbjct: 128 HIPPITI--DDEPDYCPLAIHKAILTKDPSLYPHGFTIVAIYSSYRRLCLMEAKDKRWIY 187

Query: 189 ENSEEKRHYVEDVNVCDNTLYALAYGDKGLTLGKVEVENNSDDGSSSIIALKMVTPDFFV 248
            N      + +D+NV D+ LY L +   GL + KVE        +SSI   K++    FV
Sbjct: 188 HNLNRTNGF-DDINVLDDVLYVLDW-RIGLWIAKVE--------NSSIYLKKVIFSIDFV 247

Query: 249 DDPNDLAYFAESSKKELLLIRRLFSIEKESEQGYSKIACVNTIKFLVYKLTH----GMQR 308
           +   D+AY   SSK+ELLL+ R    E+E +     +    T KF+VYK+T     G   
Sbjct: 248 E--ADIAYLVVSSKRELLLVSRFVIDEREGQDPDGIMLFTRTSKFIVYKVTRECEDGTMS 307

Query: 309 FVEVDSLDGDAMFIGDNQSTCVSTKDFPQCLPNRIYYTDSS-----PYLCPPRDVGIYNL 368
            VEV+SLDGDAMFIGDNQS C ST+DFP+CLP+R+Y+TD+      PYL   +D GIY L
Sbjct: 308 VVEVESLDGDAMFIGDNQSICASTRDFPKCLPDRVYFTDNCHHYRFPYLHGHQDAGIYRL 367

Query: 369 EDKSFGKHYITDDAHKDLPPPIWIVPTICSKGFKE 395
           EDKSF +HYI D AH++LPPPIWI+PTIC + F++
Sbjct: 368 EDKSFDRHYIVDSAHRNLPPPIWIIPTICYRDFED 388

BLAST of Lag0017649 vs. ExPASy TrEMBL
Match: A0A0A0L2J8 (DUF295 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G099610 PE=4 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 2.1e-113
Identity = 221/395 (55.95%), Postives = 274/395 (69.37%), Query Frame = 0

Query: 9   DWAALEYDVLGLILNKTVSLYDYLHFSLVCKSWNFIASRYKHQPSIITSKFSQLPMLIVP 68
           DW+ LEYDVLG+ILNK VSLYDYL FS VCKSWNFIA RYKH  S+ITS   QLPMLIVP
Sbjct: 8   DWSTLEYDVLGMILNKMVSLYDYLQFSSVCKSWNFIALRYKHHRSLITSNLPQLPMLIVP 67

Query: 69  SKHGTQKQHSLYHLPTNHIRSVDFKFCFDKRCCGSSFGWLIMLEETLDITLFNPFLGSVI 128
           S++ + KQH LY L  N IR VDF   F+KRCCGSSFGWLIMLE+TLDITLFNPF G+VI
Sbjct: 68  SEYDSGKQHCLYDLINNEIRPVDFVCSFNKRCCGSSFGWLIMLEDTLDITLFNPFNGNVI 127

Query: 129 RFPAIDLYPDNPLVYTPKSIVRAIVTKDPSLYPNDYMVVVIYSSFHQLCLMKPNDKAWIY 188
             P I ++ D P  Y+P +I +AI+TKDPS+YP  + +V IYSSF +LCLM+  DK WIY
Sbjct: 128 HIPPITIH-DEP-GYSPIAIHKAILTKDPSIYPQGFTIVAIYSSFRRLCLMEAKDKRWIY 187

Query: 189 ENSEEKRHYVEDVNVCDNTLYALAYGDKGLTLGKVEVENNSDDGSSSIIALKMVTPDFFV 248
            N      + +DVNV D+ LY L   D  + L  V+VE++S       I LK V   F  
Sbjct: 188 HNLNRNNGF-DDVNVVDDILYVL---DWRIGLWIVKVEDSS-------IYLKKVIFSFNF 247

Query: 249 DDPNDLAYFAESSKKELLLIRRLFSIEKESEQGYSKIACVNTIKFLVYKLTH----GMQR 308
           ++  D+AY   SSK+ELLL+ R    + E +     +    T KF+V+K+TH    G+ R
Sbjct: 248 NEA-DIAYLIVSSKRELLLVSRFVVTQWEGQVPNEILKFTRTSKFIVHKVTHECEDGIMR 307

Query: 309 FVEVDSLDGDAMFIGDNQSTCVSTKDFPQCLPNRIYYTDSS-----PYLCPPRDVGIYNL 368
            VEV+SLDGD MFIGDNQS CVSTKDFP+CLP+RIY+TD+      PYL   +D GIY +
Sbjct: 308 VVEVESLDGDVMFIGDNQSICVSTKDFPKCLPDRIYFTDNCHHFAFPYLHGHQDAGIYRV 367

Query: 369 EDKSFGKHYITDDAHKDLPPPIWIVPTICSKGFKE 395
           EDKSF +HYI + AH++LPPPIWI+PTI  K  ++
Sbjct: 368 EDKSFDRHYIVNRAHRNLPPPIWIIPTISYKHLED 388

BLAST of Lag0017649 vs. ExPASy TrEMBL
Match: A0A1S3BM31 (uncharacterized protein LOC103491129 OS=Cucumis melo OX=3656 GN=LOC103491129 PE=4 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 1.3e-112
Identity = 228/401 (56.86%), Postives = 278/401 (69.33%), Query Frame = 0

Query: 1   MDFSEYFGDWAALEYDVLGLILNKTVSLYDYLHFSLVCKSWNFIASRYKHQPSIITSKFS 60
           MD SE   DW  LEYD+LG+ILNK +SLYDYL FSLVCKSWNF+A R+KHQ S+ITS+F 
Sbjct: 1   MDCSE-SRDWTTLEYDILGVILNKMMSLYDYLQFSLVCKSWNFVALRHKHQRSVITSEFP 60

Query: 61  QLPMLIVPS-KHGTQKQHSLYHLPTNHIRSVDFKFCFDKRCCGSSFGWLIMLEETLDITL 120
           QLPMLIVPS K G +KQH LY +  N IR+ DFKFCF+KRCCGSSFGWLIM EET D+TL
Sbjct: 61  QLPMLIVPSIKDGIEKQHCLYDVTNNKIRTADFKFCFNKRCCGSSFGWLIMHEETFDVTL 120

Query: 121 FNPFLGSVIRFPAIDLYPDNPLVYTPKSIVRAIVTKDPSLYPNDYMVVVIYSSFHQLCLM 180
           FNPF G+VIR P +    ++   Y P  I++AI+TKDPSLYPNDYMVV IY    +LCL+
Sbjct: 121 FNPFSGTVIRLPPLPKEHEDD-DYYPWYILKAILTKDPSLYPNDYMVVAIYGISTKLCLI 180

Query: 181 KPNDKAWIYENSEEKRHY--VEDVNVCDNTLYALAYGDKGLTLGKVEVENNSDDGSSSII 240
           +   K W   +    + Y   EDV VC++TLYA    D  L L KVEV+ NS      + 
Sbjct: 181 EAKSKIWKKYDIPPGQDYNPFEDVYVCNDTLYASHLDDVQLNLWKVEVDENSPISLQRVA 240

Query: 241 ALKMVTPDFFVDDPNDLAYFAESSKKELLLIRRLFSIEKESEQGYSKI--ACVNTIKFLV 300
            +  + P     D   + +  ESSKKELLLIRR+ S+E + EQ  S I    V TIKF+ 
Sbjct: 241 II--LQPSLSTHDFGPM-FIVESSKKELLLIRRILSVE-DHEQPDSDITTTLVKTIKFVA 300

Query: 301 YKLTH----GMQRFVEVDSLDGDAMFIGDNQSTCVSTKDFPQCLPNRIYYTDS-----SP 360
           YK TH    G QRF EV SLD DA+FIG+ QS C+STK+FP+CLPN IYYTD+      P
Sbjct: 301 YKHTHTCNDGTQRFEEVKSLDDDAVFIGE-QSICISTKNFPKCLPNCIYYTDNRYYSHDP 360

Query: 361 YLCPPRDVGIYNLEDKSFGKHYITDDAHKDLPPPIWIVPTI 388
           ++  P+D+GIYNLED SFG+HYI + AH++LP PIWIVPTI
Sbjct: 361 FVNGPQDIGIYNLEDGSFGEHYIPNIAHQNLPSPIWIVPTI 394

BLAST of Lag0017649 vs. ExPASy TrEMBL
Match: A0A6J1E5P5 (probable F-box protein At1g65740 OS=Cucurbita moschata OX=3662 GN=LOC111431012 PE=4 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 1.3e-110
Identity = 215/392 (54.85%), Postives = 268/392 (68.37%), Query Frame = 0

Query: 9   DWAALEYDVLGLILNKTVSLYDYLHFSLVCKSWNFIASRYKHQPSIITSKFSQLPMLIVP 68
           DW AL+YDVLG+ILNK VSLYDYL FSLVCKSW F+A  +KHQ SII SK  QLPMLIVP
Sbjct: 8   DWTALDYDVLGVILNKMVSLYDYLQFSLVCKSWYFLALHHKHQRSIIASKVPQLPMLIVP 67

Query: 69  SKHGTQKQHSLYHLPTNHIRSVDFKFCFDKRCCGSSFGWLIMLEETLDITLFNPFLGSVI 128
           SK G +KQH LY L TN +RSVDFKFCF+KRCCGSSFGWLIM +ETL I LFNPF G+VI
Sbjct: 68  SKDGLEKQHCLYDLTTNIVRSVDFKFCFNKRCCGSSFGWLIMRDETLGIVLFNPFSGAVI 127

Query: 129 RFPAIDLYPDNPLVYTPKSIVRAIVTKDPSLYPNDYMVVVIYSSFHQLCLMKPNDKAWIY 188
             P +  Y D  + + P  I++AI+TKDPSLYPNDYMVV I+ ++ +LCL++ NDK W Y
Sbjct: 128 YLPPVATYDD--IEWIPGHIIKAILTKDPSLYPNDYMVVGIFGTYKRLCLIRANDKTWTY 187

Query: 189 ENSEEKRHYVEDVNVCDNTLYALAYGDKGLTLGKVEVENNSDDGSSSIIALKMVTPDFFV 248
                   +  D+N+C +T+YA    +  L + KVEV+N+ D+ SS  I++KMV      
Sbjct: 188 YYPTPNIPF-NDINICRDTIYAT---ENDLDVWKVEVQNSEDESSSISISVKMVHTKRI- 247

Query: 249 DDPNDLAYFAESSKKELLLIRRLFSIEKESEQGYSKIACVNTIKFLVYKLTH----GMQR 308
                 AY  ESSK ELL++ R+F+  +E            T+ F VYKL H     ++R
Sbjct: 248 -RYIGTAYITESSKGELLVVLRIFNSGEEQ---------FETVDFEVYKLIHENEDELRR 307

Query: 309 FVEVDSLDGDAMFIGDNQSTCVSTKDFPQCLPNRIYYTDSS------PYLCPPRDVGIYN 368
            VEV SLDGDA+FIGD+QS CVSTK+F  CLPNRIY+TD+         L   +D+G++N
Sbjct: 308 AVEVKSLDGDAIFIGDSQSICVSTKEFAGCLPNRIYFTDNRYVNLVVGKLDGTQDLGVFN 367

Query: 369 LEDKSFGKHYITDDAHKDLPPPIWIVPTICSK 391
            ED SF  HYI D A K+LP P+WI+PTICSK
Sbjct: 368 FEDGSFEDHYIADPAQKNLPSPMWIIPTICSK 382

BLAST of Lag0017649 vs. TAIR 10
Match: AT2G17030.1 (F-box family protein with a domain of unknown function (DUF295) )

HSP 1 Score: 67.4 bits (163), Expect = 2.9e-11
Identity = 99/417 (23.74%), Postives = 166/417 (39.81%), Query Frame = 0

Query: 9   DWAALEYDVLGLILNKTVSLYDYLHFSLVCKSWNFIASRYKHQPSIITSKFSQLPMLIVP 68
           DW+ L  D+L LI     S +D + F  VC SW   A      P+        LP  I+P
Sbjct: 3   DWSTLPKDLLDLISKSLESSFDLIQFRSVCSSWRSAAEPKSPLPT------HHLP--ILP 62

Query: 69  SKHGTQKQHSLYHLPTNHIRSVDFKFCFDKRCCGSSFGWLIMLEETLD----ITLFNPFL 128
              G+    S      +  RS+      +      SFGWLI +EE L+    +TL +P  
Sbjct: 63  DNGGSLFPDSAVGFRLSQ-RSILLIKPHEPCIESDSFGWLIKVEEDLNVPRKVTLLDPLC 122

Query: 129 GS-------------VIRFPAIDLYPDNPLVY--TPKSIVRAIVTKDPSL----YPNDYM 188
            +             + +F   +L  +  L Y  T   IV ++  +   +       DY 
Sbjct: 123 DTRNSIPENFPRVLDMSKFKVRELGREFKLHYFNTVGDIVESLYLEKAVVKYLDCDGDYK 182

Query: 189 VVVIYSSFH---QLCLMKPNDKAWIYENSEEKRHYVEDVNVCDNTLYALAYGDKGLTLGK 248
            V++  + H   +L + +  D+AW   N     +  +DV + D   +A+   + G T   
Sbjct: 183 FVLL--TIHVSGKLAVFRSWDRAWTVINDMPSPY--DDVMLFDGRFFAV--DNNGRT--- 242

Query: 249 VEVENNSDDGSSSIIALKMVTPDFFVDDPNDLAYFAESSKKELLLIRRLFSIEK-ESEQG 308
           V V+ +S       + L +V    F  D      F   S  E+LL+    S+E  E + G
Sbjct: 243 VVVDYSS-------LKLTLVASPVFGGDKK----FLIESCGEMLLVDMYLSLEAVEGDPG 302

Query: 309 YSK--------IACVNTIKFLVYKLTHGMQRFVEVDSLDGDAMFIGDNQSTCVSTKD-FP 368
           + +             T+KF VY+     + +V+V  L+   +F+GD+ +   S  D  P
Sbjct: 303 FVEEIFEHPAFYMNERTVKFKVYRFVEREESWVDVYDLEDKMLFLGDDSTFSASASDILP 362

Query: 369 QCLPNRIYYTDS-----SPYLCPPRDVGIYNLED-KSFGKHYITDDAHKDLPPPIWI 384
            C  + +++  +            RD+G+++    K      + + A    PPP WI
Sbjct: 363 LCDGSSVFFNGNVFNGEDLGAMQDRDLGVFDFRSGKIELVQKLPEYAKLFWPPPPWI 390

BLAST of Lag0017649 vs. TAIR 10
Match: AT2G17036.1 (F-box family protein with a domain of unknown function (DUF295) )

HSP 1 Score: 63.2 bits (152), Expect = 5.5e-10
Identity = 91/424 (21.46%), Postives = 161/424 (37.97%), Query Frame = 0

Query: 9   DWAALEYDVLGLILNKTVSLYDYLHFSLVCKSWNFIASRYK-----HQPSIITSKFSQLP 68
           DWA L  D+L LI     S +D + F  VC SW   A   +     + P   +     L 
Sbjct: 2   DWATLPKDLLDLISKCLESSFDLIQFRSVCSSWRSAAGPKRLLWAHNLPFFPSDDKPFLS 61

Query: 69  MLIVPSKHGTQKQHSLYHLPTNHIRSVDFKFCFDKRCCGSSFGWLIMLEETL----DITL 128
            +I+   H      S+  +  N           + +C    FGW++ + + +     +TL
Sbjct: 62  NVILRVAH-----QSILLIKPN-----------EPQCEADLFGWIVKVWDNIYVSRKMTL 121

Query: 129 FNPFLGSVIRFPAIDLYPDNPLVYTPKSIVRAIVTKDPSLY------------------- 188
             P   S   FP       +   +T + + R +    P  Y                   
Sbjct: 122 LKPLSSSRNYFPQHLPRIFDMSKFTVRELCREVKLYHPDYYCVPGHTALELELGKTVVKY 181

Query: 189 -PNDYMVVVIYSSFHQLCLMKPNDKAWIYENSEEKRHYVEDVNVCDNTLYALAYGDKGLT 248
             +D  V++    + +L + +  D+ W   N        +D+ + D   +A+ Y  + + 
Sbjct: 182 LNDDKFVLLTILEYGKLAVFRSWDREWTVINDYIPSR-CQDLIMFDGRFFAIDYNGRTVV 241

Query: 249 LGKVEVENNSDDGSSSIIALKMVTPDFFVDDPNDLAYFAESSKKELLLIRRLFSIEKESE 308
           +          D SS  + L    P     D      F   S  E+ L+   F + ++ E
Sbjct: 242 V----------DYSSFKLTL-AANPLIGGGDKK----FLIESCGEMFLVDIEFCLNEKPE 301

Query: 309 --QGYSKIACVNTI--KFLVYKLTHGMQRFVEVDSLDGDAMFIGDNQSTCVSTKD-FPQC 368
              G+       T+  KF  +KL    +R+VEV+ L     F+GD+ +   ST D  P+C
Sbjct: 302 FTGGFYSYFNETTVSYKFKFFKLVEREKRWVEVEDLGDKMFFLGDDSTFSASTADIIPRC 361

Query: 369 LPNR---IYYT-DSSPYLCPPRDVGIYNLED-KSFGKHYITDDAHKDLPPPIWIVPTICS 394
           +       +YT + S  +   R++G+++    K+   + + + A    PPP WI  +   
Sbjct: 362 VGTGSFVFFYTHEESLVVMDDRNLGVFDFRSGKTELVNKLPEYAKLFWPPPPWITTSHEV 393

BLAST of Lag0017649 vs. TAIR 10
Match: AT2G26160.1 (F-box family protein with a domain of unknown function (DUF295) )

HSP 1 Score: 55.5 bits (132), Expect = 1.2e-07
Identity = 73/356 (20.51%), Postives = 142/356 (39.89%), Query Frame = 0

Query: 9   DWAALEYDVLGLILNKTVSLYDYLHFSLVCKSWNFIASRYKHQPSIITSKFSQLPMLIVP 68
           +W+ L  D++ L  N+  S+ D L    +CK W   A+  K     + S    +  ++  
Sbjct: 5   EWSELPGDLINLTANRFSSISDVLRVRSICKPWRSAAATPKSFQCNLPSSNKMIETVL-- 64

Query: 69  SKHGTQKQHSLYHLPTNHIRSVDFKFCFDKRCCGSSFGWLI---MLEETLDITLFNPFLG 128
                         PT   R      C  K       GWLI    + E+  I L +PF  
Sbjct: 65  -------------SPTTFFRVTGPSSCSYK-------GWLIRTKQVSESSKINLLSPFFR 124

Query: 129 ----------SVIRFPAIDLYPDNPLVYTPKSIVRAIVTKDPSLYPNDYMVVVIYSSFHQ 188
                      +++F   ++     +    K +++ ++ K+    P+  +  V++   + 
Sbjct: 125 QLLTPSQQTLDLLKFEVSEIRQSYEIHIFDKYLIQGVIGKEG---PSHILSRVVFLD-NL 184

Query: 189 LCLMKPNDKAWIYENSEE-KRHYVEDVNVCDNTLYALAYGDKGLTLGKVEVENNSDDGSS 248
           +  +  +DK W  ++ EE  R + +  N  ++ L  + +  KG     +++       S 
Sbjct: 185 IFAVGQDDKIWCCKSGEESSRIWTKIKNQVEDFLDIILH--KGQVYA-LDLTGAIWWISL 244

Query: 249 SIIALKMVTPDFFVDDPNDLAYFAESSKK------ELLLIRRLFSIEKESEQGYSKIACV 308
           S ++L   TP      P D   +   +K+      +L +I +L       ++ Y +    
Sbjct: 245 SPLSLLQFTPSI----PMDYDGYDSCNKRLVEYCGDLCIIHQL-----RLKKAYIR-RSQ 304

Query: 309 NTIKFLVYKLTHGMQRFVEVDSLDGDAMFIGDNQSTCVSTKDFPQCLPNRIYYTDS 345
            T+ F VYK+   + ++VEV SL   A+ +  +    V   ++  CL N IY+ D+
Sbjct: 305 RTVGFKVYKMDEYVAKWVEVRSLGDKALIVARDSCFTVVASEYHGCLNNSIYFVDN 321

BLAST of Lag0017649 vs. TAIR 10
Match: AT2G17690.1 (F-box family protein with a domain of unknown function (DUF295) )

HSP 1 Score: 49.7 bits (117), Expect = 6.3e-06
Identity = 28/74 (37.84%), Postives = 39/74 (52.70%), Query Frame = 0

Query: 290 TIKFLVYKLTHGMQRFVEVDSLDGDAMFIGDNQSTCVSTKDFPQCLPNRIYYTDSSPYLC 349
           TI F VYK    + ++VEV SL   A+ I  +    VS  +F  CLPN IY+TD      
Sbjct: 333 TIGFKVYKNDEELLKWVEVKSLGDKAIVIATDACFSVSAHEFYGCLPNSIYFTDKK---- 392

Query: 350 PPRDVGIYNLEDKS 364
              +V ++ L+D S
Sbjct: 393 -EEEVKVFKLDDGS 401


HSP 2 Score: 39.7 bits (91), Expect = 6.6e-03
Identity = 23/75 (30.67%), Postives = 37/75 (49.33%), Query Frame = 0

Query: 8  GDWAALEYDVLGLILNKTVSLYDYLHFSLVCKSWNFIASRYKHQPSIITSKFSQLPMLIV 67
          GDW+ L  ++LGLI  +  S+ + + F  +CKSW   AS      S+ +      P+ I+
Sbjct: 2  GDWSKLPEELLGLIALRLYSVIELIRFRSICKSWRSSASGVNKNHSLSSPLIYFKPLQII 61

Query: 68 PSKHGTQKQ-HSLYH 82
           ++     Q  S YH
Sbjct: 62 LARAQANGQILSKYH 76

BLAST of Lag0017649 vs. TAIR 10
Match: AT1G65760.1 (Protein of unknown function (DUF295) )

HSP 1 Score: 43.9 bits (102), Expect = 3.5e-04
Identity = 20/65 (30.77%), Postives = 36/65 (55.38%), Query Frame = 0

Query: 290 TIKFLVYKLTHGMQRFVEVDSLDGDAMFIGDNQSTCVSTKDFPQCLPNRIYYTDSS-PYL 349
           T+ F  YK+   + +++E+ +L  +A  +G +    V   +   CLPN IYYT+++ P +
Sbjct: 272 TVGFKAYKMDEELAKWIEIKTLGDNAFLMGTDTCFSVLAHEVYGCLPNSIYYTENTWPKV 331

Query: 350 CPPRD 354
           C   D
Sbjct: 332 CKLED 336

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022923266.17.5e-11858.54probable F-box protein At1g65740 [Cucurbita moschata][more]
XP_023552957.14.9e-11758.79probable F-box protein At1g65740 [Cucurbita pepo subsp. pepo][more]
KAG6577578.14.9e-11758.29putative F-box protein, partial [Cucurbita argyrosperma subsp. sororia][more]
KAA0046890.11.2e-11555.70F-box protein SKIP23-like [Cucumis melo var. makuwa][more]
KGN56200.14.3e-11355.95hypothetical protein Csa_011053 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q3EBZ24.1e-1023.74F-box protein SKIP23 OS=Arabidopsis thaliana OX=3702 GN=SKIP23 PE=1 SV=1[more]
Q6DR205.1e-0821.50F-box protein At2g17036 OS=Arabidopsis thaliana OX=3702 GN=At2g17036 PE=2 SV=1[more]
Q1PEZ81.6e-0620.51F-box protein At2g26160 OS=Arabidopsis thaliana OX=3702 GN=At2g26160 PE=2 SV=1[more]
Q3EBY88.9e-0537.84F-box protein At2g17690 OS=Arabidopsis thaliana OX=3702 GN=SDC PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1E6C73.6e-11858.54probable F-box protein At1g65740 OS=Cucurbita moschata OX=3662 GN=LOC111431011 P... [more]
A0A5A7TUR95.8e-11655.70F-box protein SKIP23-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
A0A0A0L2J82.1e-11355.95DUF295 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G099610 PE=4... [more]
A0A1S3BM311.3e-11256.86uncharacterized protein LOC103491129 OS=Cucumis melo OX=3656 GN=LOC103491129 PE=... [more]
A0A6J1E5P51.3e-11054.85probable F-box protein At1g65740 OS=Cucurbita moschata OX=3662 GN=LOC111431012 P... [more]
Match NameE-valueIdentityDescription
AT2G17030.12.9e-1123.74F-box family protein with a domain of unknown function (DUF295) [more]
AT2G17036.15.5e-1021.46F-box family protein with a domain of unknown function (DUF295) [more]
AT2G26160.11.2e-0720.51F-box family protein with a domain of unknown function (DUF295) [more]
AT2G17690.16.3e-0637.84F-box family protein with a domain of unknown function (DUF295) [more]
AT1G65760.13.5e-0430.77Protein of unknown function (DUF295) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005174Domain unknown function DUF295PFAMPF03478DUF295coord: 306..359
e-value: 3.7E-15
score: 55.3
NoneNo IPR availableGENE3D1.20.1280.50coord: 9..49
e-value: 1.3E-5
score: 26.9
NoneNo IPR availablePANTHERPTHR44259:SF18UBIQUITIN-PROTEIN LIGASEcoord: 9..385
NoneNo IPR availablePANTHERPTHR44259OS07G0183000 PROTEIN-RELATEDcoord: 9..385

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0017649.1Lag0017649.1mRNA