Lag0017528 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0017528
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr5: 4904781 .. 4906168 (-)
RNA-Seq ExpressionLag0017528
SyntenyLag0017528
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCCCATGGAGCACAATGCAGAATCAGAGACGAGATCAAGGGAAAGAGACTGACAGCGTGACCAGCAAGATCATGGGGCTCAAACCTGCTGATCGTACAATTTTGAGAAGCCCAGAGTCAAGCACACTTAAGGGACGTGACTACACAGTTTCTACCCCAAGCTACGGTCATACTAAGACAGACCTGAGGAATCTGATCGAGGAAAAGCGCAGAATGACCAAAACTGCCGAGTCCGAGGCCAAAGCTGCAGAAGCCGAAGCCAGGGCTGCGAGGCGAGGACAGAGCAGCCGAGGCGAGGCTAGATTGGCTAAGGTCGAGGCCAAGAAAGACAATCTCCCTTGGAAGACTGAGCTTCTAAACACACTAAAGGAGCTTAGAAATCCACAAGGAGACCTGCAGAAGTCAAAGGACTCAAAAGAGCAAGACATGGAAGAACTAATCGACCAAGTCGACCCACCTTTCACAGAAGAAGTCATGAAAGCTGAGGTGCCCCAGAAGTTCAAGGTACCTACATTCAAGCCGTATGATGGCAAGTAAGACCCTGTCTAGCATCTAAATGCCTACAAAAGTTGGATGGACTTCCACGCGTCTCAGATGCAATCAGGTGTCGTGTTTTTTTTTCACCCTAACAGGATCAGCCAAGCATTGGTTTGAAAGGTTGAAAAGGAGGTCCATCATTCTTTCAAGGAATTAGCCCGAGCATTCCTTGCACAATTTATGGGAGCCAGAGAACTGCACAAGTCTCACATCAACCTCTTAACAGTCAAACAGCAGCCAGGTGAGAGCTTGCGTGATTACTAACGCGTTTTAATGACGAAGCACTATAGGTTGAGGGTACAGTGAGGAAGCAGCCCTAGTAGCCATCACAGCCGGATTGGAGGATGAGAGACTGCTCAATTCAATAGGTAAGAGCCAACCTGCCGAGTTTGTTTCCAAGGCACAGAAGTATATGAGTGCATAGGAGCTACTGAAATCAAAAAGGTCAGAACGAGAGCACAAACGACATTCTTCAGTCGATCAGGACAGAAAGAAAGACAAGAGACCGCAGACAGACGATAGTGGCCGAGGTCGAACCGACCATGACCATGGCCGAGGCCGAGCACATCCTTTCGGTAATTTGAGAAATACACCCCAACTGCTGTTCCACAGGAGCAAGTACTAATGGAGATCCGAAATACGGGCCTCCTGAATTTCCCAAGGAGGATGAAGTGAGTGCCGATAGAAGAGACAAGAGCCAATATTGCCTTCTCCATTGGGACCATGGACATTCAACCAGGAATTGTATTCAGTTGAAGGATGAGATCGAAGCACTGATCCAGAATGAGTATTTGAAAGAGTTCGTCAGTGAGCCAAGGCCGAGGCTGACCAGGGATGGCCGAGGCTAA

mRNA sequence

ATGAGCCCATGGAGCACAATGCAGAATCAGAGACGAGATCAAGGGAAAGAGACTGACAGCGTGACCAGCAAGATCATGGGGCTCAAACCTGCTGATCGTACAATTTTGAGAAGCCCAGAGTCAAGCACACTTAAGGGACGTGACTACACAGTTTCTACCCCAAGCTACGGTCATACTAAGACAGACCTGAGGAATCTGATCGAGGAAAAGCGCAGAATGACCAAAACTGCCGAGTCCGAGGCCAAAGCTGCAGAAGCCGAAGCCAGGGCTGCGAGGCGAGGACAGAGCAGCCGAGGCGAGGCTAGATTGGCTAAGGTCGAGGCCAAGAAAGACAATCTCCCTTGGAAGACTGAGCTTCTAAACACACTAAAGGAGCTTAGAAATCCACAAGGAGACCTGCAGAAGTCAAAGGACTCAAAAGAGCAAGACATGGAAGAACTAATCGACCAAGTCGACCCACCTTTCACAGAAGAAGTCATGAAAGCTGAGGTGCCCCAGAAGTTCAAGGTACCTACATTCAAGCCGTATGATGGCAATGAGGAAGCAGCCCTAGTAGCCATCACAGCCGGATTGGAGGATGAGAGACTGCTCAATTCAATAGGAGCAAGTACTAATGGAGATCCGAAATACGGGCCTCCTGAATTTCCCAAGGAGGATGAAGTGAGTGCCGATAGAAGAGACAAGAGCCAATATTGCCTTCTCCATTGGGACCATGGACATTCAACCAGGAATTGTATTCAGTTGAAGGATGAGATCGAAGCACTGATCCAGAATGAGTATTTGAAAGAGTTCGTCAGTGAGCCAAGGCCGAGGCTGACCAGGGATGGCCGAGGCTAA

Coding sequence (CDS)

ATGAGCCCATGGAGCACAATGCAGAATCAGAGACGAGATCAAGGGAAAGAGACTGACAGCGTGACCAGCAAGATCATGGGGCTCAAACCTGCTGATCGTACAATTTTGAGAAGCCCAGAGTCAAGCACACTTAAGGGACGTGACTACACAGTTTCTACCCCAAGCTACGGTCATACTAAGACAGACCTGAGGAATCTGATCGAGGAAAAGCGCAGAATGACCAAAACTGCCGAGTCCGAGGCCAAAGCTGCAGAAGCCGAAGCCAGGGCTGCGAGGCGAGGACAGAGCAGCCGAGGCGAGGCTAGATTGGCTAAGGTCGAGGCCAAGAAAGACAATCTCCCTTGGAAGACTGAGCTTCTAAACACACTAAAGGAGCTTAGAAATCCACAAGGAGACCTGCAGAAGTCAAAGGACTCAAAAGAGCAAGACATGGAAGAACTAATCGACCAAGTCGACCCACCTTTCACAGAAGAAGTCATGAAAGCTGAGGTGCCCCAGAAGTTCAAGGTACCTACATTCAAGCCGTATGATGGCAATGAGGAAGCAGCCCTAGTAGCCATCACAGCCGGATTGGAGGATGAGAGACTGCTCAATTCAATAGGAGCAAGTACTAATGGAGATCCGAAATACGGGCCTCCTGAATTTCCCAAGGAGGATGAAGTGAGTGCCGATAGAAGAGACAAGAGCCAATATTGCCTTCTCCATTGGGACCATGGACATTCAACCAGGAATTGTATTCAGTTGAAGGATGAGATCGAAGCACTGATCCAGAATGAGTATTTGAAAGAGTTCGTCAGTGAGCCAAGGCCGAGGCTGACCAGGGATGGCCGAGGCTAA

Protein sequence

MSPWSTMQNQRRDQGKETDSVTSKIMGLKPADRTILRSPESSTLKGRDYTVSTPSYGHTKTDLRNLIEEKRRMTKTAESEAKAAEAEARAARRGQSSRGEARLAKVEAKKDNLPWKTELLNTLKELRNPQGDLQKSKDSKEQDMEELIDQVDPPFTEEVMKAEVPQKFKVPTFKPYDGNEEAALVAITAGLEDERLLNSIGASTNGDPKYGPPEFPKEDEVSADRRDKSQYCLLHWDHGHSTRNCIQLKDEIEALIQNEYLKEFVSEPRPRLTRDGRG
Homology
BLAST of Lag0017528 vs. NCBI nr
Match: XP_022142767.1 (uncharacterized protein LOC111012805 [Momordica charantia])

HSP 1 Score: 73.6 bits (179), Expect = 3.1e-09
Identity = 28/56 (50.00%), Postives = 41/56 (73.21%), Query Frame = 0

Query: 214 EFPKEDEVSADRRDKSQYCLLHWDHGHSTRNCIQLKDEIEALIQNEYLKEFVSEPR 270
           ++P+    S+ +R K +YCL HWDHGH+T++C  LK+E+E LI   YLKE+V EP+
Sbjct: 245 KWPERMNASSTKRSKGRYCLFHWDHGHATQDCFDLKEEVEGLILRGYLKEYVEEPK 300

BLAST of Lag0017528 vs. NCBI nr
Match: GFY81140.1 (hypothetical protein Acr_01g0009490 [Actinidia rufa])

HSP 1 Score: 70.9 bits (172), Expect = 2.0e-08
Identity = 41/149 (27.52%), Postives = 74/149 (49.66%), Query Frame = 0

Query: 144 MEELIDQVDPPFTEEVMKAEVPQKFKVPTFKPYDGNEEAALVAITAGLEDERLLNSIGAS 203
           ++ L+ Q +PPFT  +++A V  +FK+PT         + L  I    E ERL + +   
Sbjct: 88  VDALVRQTEPPFTARILRARVSSRFKLPTQLGVRKKNASHLFTIHQ-KETERLKDYVKQF 147

Query: 204 TNGDPKYGPP---------------EFPKEDEVSADRRDKSQYCLLHWDHGHSTRNCIQL 263
                +   P               ++P++ +    RR+K +YC  HWDH H+T +C QL
Sbjct: 148 NQAILEVEDPSDKVVLTEIKHKEFVKWPRKIKTDPSRRNKGKYCEFHWDHRHNTDDCFQL 207

Query: 264 KDEIEALIQNEYLKEFVSEPRPRLTRDGR 278
           K+ I  LI+  +L+++V+  +  ++ + R
Sbjct: 208 KEHIADLIKKGFLRKYVANRQSPISPERR 235

BLAST of Lag0017528 vs. NCBI nr
Match: XP_022158830.1 (uncharacterized protein LOC111025293 [Momordica charantia])

HSP 1 Score: 69.3 bits (168), Expect = 5.9e-08
Identity = 27/56 (48.21%), Postives = 42/56 (75.00%), Query Frame = 0

Query: 214 EFPKEDEVSADRRDKSQYCLLHWDHGHSTRNCIQLKDEIEALIQNEYLKEFVSEPR 270
           ++P+  + S+ +R K +YCL H DHGH+T++C  LK+E+E LI+  YLKE+V EP+
Sbjct: 420 KWPERMKASSAKRSKGRYCLFHRDHGHATQDCFDLKEEVEGLIRRGYLKEYVEEPK 475

BLAST of Lag0017528 vs. NCBI nr
Match: XP_022158830.1 (uncharacterized protein LOC111025293 [Momordica charantia])

HSP 1 Score: 50.8 bits (120), Expect = 2.2e-02
Identity = 25/51 (49.02%), Postives = 34/51 (66.67%), Query Frame = 0

Query: 127 RNPQGDLQKSKDSKEQDMEELIDQVDPPFTEEVMKAEVPQKFKVPTFKPYD 178
           R    + + S   K  D+EEL+DQ D PFTEE+M+ +VP KFK+PT K +D
Sbjct: 152 RPESSEKRHSHKEKGFDLEELLDQADSPFTEEIMREKVPPKFKLPTVKQFD 202


HSP 2 Score: 67.0 bits (162), Expect = 2.9e-07
Identity = 31/61 (50.82%), Postives = 43/61 (70.49%), Query Frame = 0

Query: 218 EDEVSADRRDKSQYCLLHWDHGHSTRNCIQLKDEIEALIQNEYLKEFVS-EPRPRLTRDG 277
           +DE++ D RD+S+YC  H DHGH+T +C  L+ EIEALI+  +LK +V  + RPR  RD 
Sbjct: 256 KDEITPDTRDRSKYCEFHRDHGHTTEDCQALQREIEALIKRGFLKNYVGHDKRPRNDRDN 315

BLAST of Lag0017528 vs. NCBI nr
Match: XP_022152370.1 (uncharacterized protein LOC111020115 [Momordica charantia])

HSP 1 Score: 66.6 bits (161), Expect = 3.8e-07
Identity = 28/54 (51.85%), Postives = 40/54 (74.07%), Query Frame = 0

Query: 216 PKEDEVSADRRDKSQYCLLHWDHGHSTRNCIQLKDEIEALIQNEYLKEFVSEPR 270
           P++     ++R+K +YCL H DHGHST NC +LK +IE LIQ++Y K+FV +PR
Sbjct: 125 PEKLRGDPEKRNKDKYCLFHRDHGHSTSNCWELKRQIEDLIQDDYFKKFVGKPR 178

BLAST of Lag0017528 vs. ExPASy TrEMBL
Match: A0A6J1CNT2 (uncharacterized protein LOC111012805 OS=Momordica charantia OX=3673 GN=LOC111012805 PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.5e-09
Identity = 28/56 (50.00%), Postives = 41/56 (73.21%), Query Frame = 0

Query: 214 EFPKEDEVSADRRDKSQYCLLHWDHGHSTRNCIQLKDEIEALIQNEYLKEFVSEPR 270
           ++P+    S+ +R K +YCL HWDHGH+T++C  LK+E+E LI   YLKE+V EP+
Sbjct: 245 KWPERMNASSTKRSKGRYCLFHWDHGHATQDCFDLKEEVEGLILRGYLKEYVEEPK 300

BLAST of Lag0017528 vs. ExPASy TrEMBL
Match: A0A7J0E3Z6 (Uncharacterized protein OS=Actinidia rufa OX=165716 GN=Acr_01g0009490 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 9.7e-09
Identity = 41/149 (27.52%), Postives = 74/149 (49.66%), Query Frame = 0

Query: 144 MEELIDQVDPPFTEEVMKAEVPQKFKVPTFKPYDGNEEAALVAITAGLEDERLLNSIGAS 203
           ++ L+ Q +PPFT  +++A V  +FK+PT         + L  I    E ERL + +   
Sbjct: 88  VDALVRQTEPPFTARILRARVSSRFKLPTQLGVRKKNASHLFTIHQ-KETERLKDYVKQF 147

Query: 204 TNGDPKYGPP---------------EFPKEDEVSADRRDKSQYCLLHWDHGHSTRNCIQL 263
                +   P               ++P++ +    RR+K +YC  HWDH H+T +C QL
Sbjct: 148 NQAILEVEDPSDKVVLTEIKHKEFVKWPRKIKTDPSRRNKGKYCEFHWDHRHNTDDCFQL 207

Query: 264 KDEIEALIQNEYLKEFVSEPRPRLTRDGR 278
           K+ I  LI+  +L+++V+  +  ++ + R
Sbjct: 208 KEHIADLIKKGFLRKYVANRQSPISPERR 235

BLAST of Lag0017528 vs. ExPASy TrEMBL
Match: A0A6J1DWY0 (uncharacterized protein LOC111025293 OS=Momordica charantia OX=3673 GN=LOC111025293 PE=4 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 2.8e-08
Identity = 27/56 (48.21%), Postives = 42/56 (75.00%), Query Frame = 0

Query: 214 EFPKEDEVSADRRDKSQYCLLHWDHGHSTRNCIQLKDEIEALIQNEYLKEFVSEPR 270
           ++P+  + S+ +R K +YCL H DHGH+T++C  LK+E+E LI+  YLKE+V EP+
Sbjct: 420 KWPERMKASSAKRSKGRYCLFHRDHGHATQDCFDLKEEVEGLIRRGYLKEYVEEPK 475

BLAST of Lag0017528 vs. ExPASy TrEMBL
Match: A0A6J1DWY0 (uncharacterized protein LOC111025293 OS=Momordica charantia OX=3673 GN=LOC111025293 PE=4 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 1.0e-02
Identity = 25/51 (49.02%), Postives = 34/51 (66.67%), Query Frame = 0

Query: 127 RNPQGDLQKSKDSKEQDMEELIDQVDPPFTEEVMKAEVPQKFKVPTFKPYD 178
           R    + + S   K  D+EEL+DQ D PFTEE+M+ +VP KFK+PT K +D
Sbjct: 152 RPESSEKRHSHKEKGFDLEELLDQADSPFTEEIMREKVPPKFKLPTVKQFD 202


HSP 2 Score: 68.2 bits (165), Expect = 6.3e-08
Identity = 43/133 (32.33%), Postives = 66/133 (49.62%), Query Frame = 0

Query: 143 DMEELIDQVDPPFTEEVMKAEVPQKFKVPTFKPYDG-------NEEAALVAITAGLEDER 202
           +++ LI + + PF + V    +P KFKVP  + +DG        +    V    G+ DE 
Sbjct: 15  NLDMLIHRSESPFIKRVNDYPLPAKFKVPQLENFDGLRDPLDYLDSLRTVMRLQGVSDEI 74

Query: 203 LLNSIGASTNGDPKYGPP-EFPKEDEVSADRRDKSQYCLLHWDHGHSTRNCIQLKDEIEA 262
           +  +   +  G  +  P  ++P     S  RR K  YC  H DHGH+T +   LK+ IEA
Sbjct: 75  MCRAFPTNLRGSIQDDPALKWPGNLRSSPGRRSKDLYCRFHRDHGHNTEDYFVLKENIEA 134

Query: 263 LIQNEYLKEFVSE 268
           LI+   LK+FV +
Sbjct: 135 LIRQGKLKKFVCQ 147

BLAST of Lag0017528 vs. ExPASy TrEMBL
Match: A0A6J1DDR5 (uncharacterized protein LOC111020115 OS=Momordica charantia OX=3673 GN=LOC111020115 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.8e-07
Identity = 28/54 (51.85%), Postives = 40/54 (74.07%), Query Frame = 0

Query: 216 PKEDEVSADRRDKSQYCLLHWDHGHSTRNCIQLKDEIEALIQNEYLKEFVSEPR 270
           P++     ++R+K +YCL H DHGHST NC +LK +IE LIQ++Y K+FV +PR
Sbjct: 125 PEKLRGDPEKRNKDKYCLFHRDHGHSTSNCWELKRQIEDLIQDDYFKKFVGKPR 178

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022142767.13.1e-0950.00uncharacterized protein LOC111012805 [Momordica charantia][more]
GFY81140.12.0e-0827.52hypothetical protein Acr_01g0009490 [Actinidia rufa][more]
XP_022158830.15.9e-0848.21uncharacterized protein LOC111025293 [Momordica charantia][more]
XP_022158830.12.2e-0249.02uncharacterized protein LOC111025293 [Momordica charantia][more]
XP_022152370.13.8e-0751.85uncharacterized protein LOC111020115 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1CNT21.5e-0950.00uncharacterized protein LOC111012805 OS=Momordica charantia OX=3673 GN=LOC111012... [more]
A0A7J0E3Z69.7e-0927.52Uncharacterized protein OS=Actinidia rufa OX=165716 GN=Acr_01g0009490 PE=4 SV=1[more]
A0A6J1DWY02.8e-0848.21uncharacterized protein LOC111025293 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A6J1DWY01.0e-0249.02uncharacterized protein LOC111025293 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A6J1DDR51.8e-0751.85uncharacterized protein LOC111020115 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 67..87
NoneNo IPR availableCOILSCoilCoilcoord: 130..150
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..105
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 199..224
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 36..60
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 130..145
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 62..105
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 124..145

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0017528.1Lag0017528.1mRNA