Lag0017525 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0017525 vs. NCBI nr
Match: KAA0039950.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK24553.1 LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 182.6 bits (462), Expect = 3.2e-42
Identity = 83/189 (43.92%), Postives = 119/189 (62.96%), Query Frame = 0

Query: 1   MIEKIQRRIHNWRFASLSKESRLTLIHSVLNSMPLYILSVFKAPTSICNKIEKKIWKFLW 60
           +++KIQ+++ NW+++ LSK  R+TLI+S L S+P+Y +SVFK P  I  KIE     FLW
Sbjct: 699 VLQKIQKKLSNWKYSQLSKGGRITLINSTLESLPIYQMSVFKVPKGIAQKIEASWRNFLW 758

Query: 61  NGASSSGITNLVRWEIIVSSSKAEGGLGIHKIKETNDALLLKWIWRFLNEENTLWRKFIN 120
           NGAS+    +L+RW  IV S K +GGLGIH +  TN ALL KW+W+FL E++ LW++ I 
Sbjct: 759 NGASNGHNISLIRWNQIV-SPKEKGGLGIHSVNSTNFALLCKWLWKFLTEKDPLWKRLII 818

Query: 121 NKYSSLQFECFPSRNNVSSSRSPWHAISKLQDVIFANFRWDIRNGGSTLSWHDNWSSFGP 180
           +KY   +   FPS    SS+ SPW A+++     + N  W + +G     W DNW+   P
Sbjct: 819 SKYDKEKMGSFPSHGKFSSNNSPWKAVTECISWFYKNISWKVNDGEDISFWLDNWNGNAP 878

Query: 181 LKLVCNRLY 190
           L L   RL+
Sbjct: 879 LSLAVPRLF 886

BLAST of Lag0017525 vs. NCBI nr
Match: KAA0041397.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 179.9 bits (455), Expect = 2.1e-41
Identity = 83/189 (43.92%), Postives = 118/189 (62.43%), Query Frame = 0

Query: 1   MIEKIQRRIHNWRFASLSKESRLTLIHSVLNSMPLYILSVFKAPTSICNKIEKKIWKFLW 60
           +++KIQ+++ +W+++ LSK  R+TLI+S L S+P+Y LSVFK P  I  KIE     FLW
Sbjct: 591 ILQKIQKKLSSWKYSQLSKGGRITLINSTLESLPIYQLSVFKVPKGIAQKIEAYWRNFLW 650

Query: 61  NGASSSGITNLVRWEIIVSSSKAEGGLGIHKIKETNDALLLKWIWRFLNEENTLWRKFIN 120
           NG S+    +L+RW  +V S K +GGLGIH +  TN ALL KW+W+FL E+  LW++ I 
Sbjct: 651 NGTSNGHNISLIRWNQVV-SPKEKGGLGIHSVHSTNFALLCKWLWKFLTEKEPLWKRLII 710

Query: 121 NKYSSLQFECFPSRNNVSSSRSPWHAISKLQDVIFANFRWDIRNGGSTLSWHDNWSSFGP 180
           +KY   +   FPSR   SS+ SPW A++      + N  W + +G     W DNW+   P
Sbjct: 711 SKYDQEKMGRFPSRGKYSSNNSPWKAVTNCISWFYKNIGWKVNDGEDISFWLDNWNGNSP 770

Query: 181 LKLVCNRLY 190
           L LV  RL+
Sbjct: 771 LSLVVPRLF 778

BLAST of Lag0017525 vs. NCBI nr
Match: KAA0044556.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 177.2 bits (448), Expect = 1.4e-40
Identity = 82/189 (43.39%), Postives = 117/189 (61.90%), Query Frame = 0

Query: 1   MIEKIQRRIHNWRFASLSKESRLTLIHSVLNSMPLYILSVFKAPTSICNKIEKKIWKFLW 60
           +++KIQ+++ +W+++ LSK  R+TLI+S L S+P+Y LSVFK P  I  KIE     FLW
Sbjct: 497 ILQKIQKKLSSWKYSQLSKGGRITLINSTLESLPIYQLSVFKVPKGIAQKIEAYWRNFLW 556

Query: 61  NGASSSGITNLVRWEIIVSSSKAEGGLGIHKIKETNDALLLKWIWRFLNEENTLWRKFIN 120
           NG S+    +L+RW  +V S K +GGLGIH +  TN ALL KW+W+FL E+  LW++ I 
Sbjct: 557 NGTSNGHNISLIRWNQVV-SPKEKGGLGIHFVHSTNFALLCKWLWKFLTEKEPLWKRLII 616

Query: 121 NKYSSLQFECFPSRNNVSSSRSPWHAISKLQDVIFANFRWDIRNGGSTLSWHDNWSSFGP 180
           +KY   +   FPSR   SS+ SPW A++      + N  W + +G     W DNW+   P
Sbjct: 617 SKYDQEKMGRFPSRGKYSSNNSPWKAVTNCISWFYKNIGWKVNDGEDISFWLDNWNGNSP 676

Query: 181 LKLVCNRLY 190
           L L   RL+
Sbjct: 677 LSLAVPRLF 684

BLAST of Lag0017525 vs. NCBI nr
Match: TYK05718.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 174.5 bits (441), Expect = 8.8e-40
Identity = 85/187 (45.45%), Postives = 118/187 (63.10%), Query Frame = 0

Query: 3   EKIQRRIHNWRFASLSKESRLTLIHSVLNSMPLYILSVFKAPTSICNKIEKKIWKFLWNG 62
           +KIQ++++NW++A +SK  RLTLI S L+S+P+Y LSVF+AP+  C  IEK   KFLW G
Sbjct: 297 DKIQKKLNNWKYAQISKGGRLTLIKSTLSSLPIYQLSVFQAPSLTCKNIEKSWRKFLWKG 356

Query: 63  ASSSGITNLVRWEIIVSSSKAEGGLGIHKIKETNDALLLKWIWRFLNEENTLWRKFINNK 122
            + S  ++L+ W   VS SK EGGLGI +++ TN ALL KW+WR+L+E N LWR+ I  K
Sbjct: 357 NNGSEGSHLINW-TKVSKSKEEGGLGISRLQVTNKALLSKWLWRYLSEPNALWRRLIQCK 416

Query: 123 YSSLQFECFPSRNNVSSSRSPWHAISKLQDVIFANFRWDIRNGGSTLSWHDNWSSFGPLK 182
           Y         S N+ S+S++PW +I    D   +N  WD+ NG     W+ NWS  G L 
Sbjct: 417 YKGKYPGDITSNNSSSTSKAPWRSIIDNIDWFKSNQSWDLNNGDQISFWYSNWSQEGRLS 476

Query: 183 LVCNRLY 190
               RL+
Sbjct: 477 TAYPRLF 482

BLAST of Lag0017525 vs. NCBI nr
Match: TYJ99315.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 173.3 bits (438), Expect = 2.0e-39
Identity = 85/187 (45.45%), Postives = 117/187 (62.57%), Query Frame = 0

Query: 3    EKIQRRIHNWRFASLSKESRLTLIHSVLNSMPLYILSVFKAPTSICNKIEKKIWKFLWNG 62
            +KIQ++++NW++A +SK  RLTLI S L+S+P+Y LSVF+AP+  C  IEK   KFLW G
Sbjct: 1439 DKIQKKLNNWKYAQISKGGRLTLIKSTLSSLPIYQLSVFQAPSLTCKNIEKLWRKFLWKG 1498

Query: 63   ASSSGITNLVRWEIIVSSSKAEGGLGIHKIKETNDALLLKWIWRFLNEENTLWRKFINNK 122
             + S  ++L+ W   VS SK EGGLGI ++  TN ALL KW+WR+L+E N LWR+ I  K
Sbjct: 1499 NNGSEGSHLINW-TKVSKSKEEGGLGISRLNVTNKALLSKWLWRYLSEPNALWRRLIQCK 1558

Query: 123  YSSLQFECFPSRNNVSSSRSPWHAISKLQDVIFANFRWDIRNGGSTLSWHDNWSSFGPLK 182
            Y        PS  + S+S++PW +I    D   +N  WD+ NG     W+ NWS  G L 
Sbjct: 1559 YKGKFPGDIPSNISSSTSKAPWRSIIDSTDWFKSNQSWDLNNGDQISFWYSNWSQEGRLS 1618

Query: 183  LVCNRLY 190
                RL+
Sbjct: 1619 TAYPRLF 1624

BLAST of Lag0017525 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 5.0e-22
Identity = 63/182 (34.62%), Postives = 92/182 (50.55%), Query Frame = 0

Query: 1   MIEKIQRRIHNWRFASLSKESRLTLIHSVLNSMPLYILSVFKAPTSICNKIEKKIWKFLW 60
           ++E++  R+  WR  +LS   RLTL  +VL+SMP++ +S    P SI N++++    FLW
Sbjct: 16  ILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQSILNRLDQLSRTFLW 75

Query: 61  NGASSSGITNLVRWEIIVSSSKAEGGLGIHKIKETNDALLLKWIWRFLNEENTLWRKFIN 120
              +     +LV+W   V S K EGGLG+   K  N AL+ K  WR L E+N+LW   + 
Sbjct: 76  GSTAEKKKQHLVKWS-KVCSPKKEGGLGVRAAKSMNRALISKVGWRLLQEKNSLWTLVLQ 135

Query: 121 NKYSSLQFECFPSRNNVSSSRSPWHAIS-KLQDVIFANFRWDIRNGGSTLSWHDNWSSFG 180
            KY   +           S  S W +I+  L+DV+     W   +G     W D W S  
Sbjct: 136 KKYHVGEIRDSRWLIPKGSWSSTWRSIAIGLRDVVSHGVGWIPGDGQQIRFWTDRWVSGK 195

Query: 181 PL 182
           PL
Sbjct: 196 PL 196

BLAST of Lag0017525 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.2e-09
Identity = 37/147 (25.17%), Postives = 71/147 (48.30%), Query Frame = 0

Query: 32  SMPLYILSVFKAPTSICNKIEKKIWKFLWNGASSSGITNLVRWEIIVSSSKAEGGLGIHK 91
           ++P+Y +S F+    +C K+   + +F W+   +    + V W+ +  S + +GGLG   
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 92  IKETNDALLLKWIWRFLNEENTLWRKFINNKY--SSLQFECFPSRNNVSSSRS-PWHAIS 151
           +   N ALL K  +R +++ +TL  + + ++Y   S   EC     +V +  S  W +I 
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMEC-----SVGTRPSYAWRSII 121

Query: 152 KLQDVIFANFRWDIRNGGSTLSWHDNW 176
             ++++       I +G  T  W D W
Sbjct: 122 HGRELLSRGLLRTIGDGIHTKVWLDRW 143