Lag0017486 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0017486
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	50S ribosomal protein L7/L12
Location	chr5: 4242096 .. 4242680 (+)
RNA-Seq Expression	Lag0017486
Synteny	Lag0017486

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCTGATTGCAGTTGCTGCACGATCTCTTCAAGTCCGTCCCACACTTGCTAAAATAATTGGGTTGTCACAGGTCCGCTTCTTCCAGCCTGATTTTACCCCCCGAGACCCAAATGCCAAGTCAAAAAGATACAAGTATCCAGCATTTTATGATCCATATGGCCCTAGGCCCTCTCCCTCTGATAAAATCATTCAGCTTGCAGAAAGAATTGTAGCACTGCCTGTGGATGAGCGTTGCCAGATTGGTCCCACACTCTCTGAAAGGCTTAGACATCCTAAGCTGCAGCAAATTTCAGTGGATGGTTTGGACATGGGTTCCGATGGAGGGGCAGCCGCAGGATCTTCTAAGGTGGAGGAGATTAAAGAAAAAACCGCTTTTGATGTGAAGTTAGAGAAGTTTGATGCAGCTGCAAAAATTAAGGTGATTAAAGAAGTAAGGTCCTTCACTAATTTGGGCTTGAAGGAGGCGAAAGATTTAGTGGAGAAAGTACCTGCAATTCTTAAGCAAGGGGTGACCAAAGAGGAGGCAAACGACATAATTGAAAAAATCAAAGCTGCTGGAGGAGTTGCAGTCATGGAGTAA

mRNA sequence

ATGAAGCTGATTGCAGTTGCTGCACGATCTCTTCAAGTCCGTCCCACACTTGCTAAAATAATTGGGTTGTCACAGGTCCGCTTCTTCCAGCCTGATTTTACCCCCCGAGACCCAAATGCCAAGTCAAAAAGATACAAGTATCCAGCATTTTATGATCCATATGGCCCTAGGCCCTCTCCCTCTGATAAAATCATTCAGCTTGCAGAAAGAATTGTAGCACTGCCTGTGGATGAGCGTTGCCAGATTGGTCCCACACTCTCTGAAAGGCTTAGACATCCTAAGCTGCAGCAAATTTCAGTGGATGGTTTGGACATGGGTTCCGATGGAGGGGCAGCCGCAGGATCTTCTAAGGTGGAGGAGATTAAAGAAAAAACCGCTTTTGATGTGAAGTTAGAGAAGTTTGATGCAGCTGCAAAAATTAAGGTGATTAAAGAAGTAAGGTCCTTCACTAATTTGGGCTTGAAGGAGGCGAAAGATTTAGTGGAGAAAGTACCTGCAATTCTTAAGCAAGGGGTGACCAAAGAGGAGGCAAACGACATAATTGAAAAAATCAAAGCTGCTGGAGGAGTTGCAGTCATGGAGTAA

Coding sequence (CDS)

ATGAAGCTGATTGCAGTTGCTGCACGATCTCTTCAAGTCCGTCCCACACTTGCTAAAATAATTGGGTTGTCACAGGTCCGCTTCTTCCAGCCTGATTTTACCCCCCGAGACCCAAATGCCAAGTCAAAAAGATACAAGTATCCAGCATTTTATGATCCATATGGCCCTAGGCCCTCTCCCTCTGATAAAATCATTCAGCTTGCAGAAAGAATTGTAGCACTGCCTGTGGATGAGCGTTGCCAGATTGGTCCCACACTCTCTGAAAGGCTTAGACATCCTAAGCTGCAGCAAATTTCAGTGGATGGTTTGGACATGGGTTCCGATGGAGGGGCAGCCGCAGGATCTTCTAAGGTGGAGGAGATTAAAGAAAAAACCGCTTTTGATGTGAAGTTAGAGAAGTTTGATGCAGCTGCAAAAATTAAGGTGATTAAAGAAGTAAGGTCCTTCACTAATTTGGGCTTGAAGGAGGCGAAAGATTTAGTGGAGAAAGTACCTGCAATTCTTAAGCAAGGGGTGACCAAAGAGGAGGCAAACGACATAATTGAAAAAATCAAAGCTGCTGGAGGAGTTGCAGTCATGGAGTAA

Protein sequence

MKLIAVAARSLQVRPTLAKIIGLSQVRFFQPDFTPRDPNAKSKRYKYPAFYDPYGPRPSPSDKIIQLAERIVALPVDERCQIGPTLSERLRHPKLQQISVDGLDMGSDGGAAAGSSKVEEIKEKTAFDVKLEKFDAAAKIKVIKEVRSFTNLGLKEAKDLVEKVPAILKQGVTKEEANDIIEKIKAAGGVAVME

Homology

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BLAST of Lag0017486 vs. NCBI nr
Match: XP_011651634.1 (uncharacterized protein LOC101206962 [Cucumis sativus] >XP_011651635.1 uncharacterized protein LOC101206962 [Cucumis sativus] >KGN58328.1 hypothetical protein Csa_017468 [Cucumis sativus])

HSP 1 Score: 327.8 bits (839), Expect = 6.4e-86
Identity = 169/191 (88.48%), Postives = 178/191 (93.19%), Query Frame = 0


Query: 4   IAVAARSLQVRPTLAKIIGLSQVRFFQPDFTPRDPNAKSKRYKYPAFYDPYGPRPSPSDK 63
           + V A+SLQVR T AKIIGLSQVRFFQPDFTPRDPNAK K+YKYPAFYDPYGPRP PSDK
Sbjct: 3   LTVVAQSLQVRSTFAKIIGLSQVRFFQPDFTPRDPNAKPKKYKYPAFYDPYGPRPPPSDK 62

Query: 64  IIQLAERIVALPVDERCQIGPTLSERLRHPKLQQISVDGLDMGSDGGAAAGSSKVEEIKE 123
           II+LAERIVALP  ERCQIGPTL E+LRHPKLQ+ISVDGLDMG +GGAAAGSS VEE KE
Sbjct: 63  IIELAERIVALPAAERCQIGPTLGEKLRHPKLQEISVDGLDMGPEGGAAAGSSNVEEKKE 122

Query: 124 KTAFDVKLEKFDAAAKIKVIKEVRSFTNLGLKEAKDLVEKVPAILKQGVTKEEANDIIEK 183
           KTAFDVKLEKFDAA+KIKVIKEVR+FTNLGLKEAKDLVEKVPAILKQGVTKEEAN IIEK
Sbjct: 123 KTAFDVKLEKFDAASKIKVIKEVRAFTNLGLKEAKDLVEKVPAILKQGVTKEEANGIIEK 182

Query: 184 IKAAGGVAVME 195
           IKAAGGVAVME
Sbjct: 183 IKAAGGVAVME 193

[Close]

BLAST of Lag0017486 vs. NCBI nr
Match: XP_022155073.1 (uncharacterized protein LOC111022207 [Momordica charantia] >XP_022155074.1 uncharacterized protein LOC111022207 [Momordica charantia])

HSP 1 Score: 326.6 bits (836), Expect = 1.4e-85
Identity = 173/194 (89.18%), Postives = 178/194 (91.75%), Query Frame = 0


Query: 1   MKLIAVAARSLQVRPTLAKIIGLSQVRFFQPDFTPRDPNAKSKRYKYPAFYDPYGPRPSP 60
           MKL+AV ARSLQV  TL K+IG  QVRFFQPDF PRD  AK KRYKYP FYDPYGPRP P
Sbjct: 1   MKLVAV-ARSLQVHSTLPKVIGSFQVRFFQPDFAPRDSTAKPKRYKYPPFYDPYGPRPPP 60

Query: 61  SDKIIQLAERIVALPVDERCQIGPTLSERLRHPKLQQISVDGLDMGSDGGAAAGSSKVEE 120
           SDKIIQLAE+IVALPVDERCQIGP L+ERLRHPKLQ ISVDGLDMGS+GGA AGSSKVEE
Sbjct: 61  SDKIIQLAEKIVALPVDERCQIGPALTERLRHPKLQPISVDGLDMGSEGGAGAGSSKVEE 120

Query: 121 IKEKTAFDVKLEKFDAAAKIKVIKEVRSFTNLGLKEAKDLVEKVPAILKQGVTKEEANDI 180
            KEKTAFDVKLEKFDAAAKIKVIKEVRSFTNLGLKEAKDLVEKVPAILKQGVTKEEANDI
Sbjct: 121 KKEKTAFDVKLEKFDAAAKIKVIKEVRSFTNLGLKEAKDLVEKVPAILKQGVTKEEANDI 180

Query: 181 IEKIKAAGGVAVME 195
           IEKIKAAGGVAVME
Sbjct: 181 IEKIKAAGGVAVME 193

[Close]

BLAST of Lag0017486 vs. NCBI nr
Match: XP_022949504.1 (uncharacterized protein LOC111452831 [Cucurbita moschata])

HSP 1 Score: 325.9 bits (834), Expect = 2.4e-85
Identity = 171/194 (88.14%), Postives = 180/194 (92.78%), Query Frame = 0


Query: 1   MKLIAVAARSLQVRPTLAKIIGLSQVRFFQPDFTPRDPNAKSKRYKYPAFYDPYGPRPSP 60
           MK+IAV A+SLQ R TL KIIGL QVRFFQPDFTPRDPNAK KRYKYP FYDPYGPRP P
Sbjct: 1   MKMIAV-AQSLQARSTLPKIIGLLQVRFFQPDFTPRDPNAKPKRYKYPPFYDPYGPRPPP 60

Query: 61  SDKIIQLAERIVALPVDERCQIGPTLSERLRHPKLQQISVDGLDMGSDGGAAAGSSKVEE 120
           S+KIIQLAERI ALPVDERCQIGP L+ERL+HPKL+QISVDG+DMGS GGAA GSSKVEE
Sbjct: 61  SNKIIQLAERIAALPVDERCQIGPALTERLKHPKLEQISVDGMDMGSGGGAATGSSKVEE 120

Query: 121 IKEKTAFDVKLEKFDAAAKIKVIKEVRSFTNLGLKEAKDLVEKVPAILKQGVTKEEANDI 180
            KEKTAFDVKLEKF+AAAKIKVIKEVR+FTNLGLKEAKDLVEKVPAILKQGVTKEEAN I
Sbjct: 121 KKEKTAFDVKLEKFEAAAKIKVIKEVRAFTNLGLKEAKDLVEKVPAILKQGVTKEEANSI 180

Query: 181 IEKIKAAGGVAVME 195
           IEKIKAAGGVAVME
Sbjct: 181 IEKIKAAGGVAVME 193

[Close]

BLAST of Lag0017486 vs. NCBI nr
Match: XP_023525921.1 (uncharacterized protein LOC111789392 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 324.3 bits (830), Expect = 7.1e-85
Identity = 170/194 (87.63%), Postives = 180/194 (92.78%), Query Frame = 0


Query: 1   MKLIAVAARSLQVRPTLAKIIGLSQVRFFQPDFTPRDPNAKSKRYKYPAFYDPYGPRPSP 60
           MK+IAV A+SLQ R TL KIIG  QVRFFQPDFTPRDPNAK K+YKYP FYDPYGPRP P
Sbjct: 1   MKMIAV-AQSLQARSTLPKIIGSLQVRFFQPDFTPRDPNAKPKKYKYPPFYDPYGPRPPP 60

Query: 61  SDKIIQLAERIVALPVDERCQIGPTLSERLRHPKLQQISVDGLDMGSDGGAAAGSSKVEE 120
           S+KIIQLAERI ALPVDERCQIGPTL+ERL+HPKL+QISVDG+DMGS GGAA GSSKVEE
Sbjct: 61  SNKIIQLAERIAALPVDERCQIGPTLTERLKHPKLEQISVDGMDMGSGGGAATGSSKVEE 120

Query: 121 IKEKTAFDVKLEKFDAAAKIKVIKEVRSFTNLGLKEAKDLVEKVPAILKQGVTKEEANDI 180
            KEKTAFDVKLEKF+AAAKIKVIKEVR+FTNLGLKEAKDLVEKVPAILKQGVTKEEAN I
Sbjct: 121 KKEKTAFDVKLEKFEAAAKIKVIKEVRAFTNLGLKEAKDLVEKVPAILKQGVTKEEANSI 180

Query: 181 IEKIKAAGGVAVME 195
           IEKIKAAGGVAVME
Sbjct: 181 IEKIKAAGGVAVME 193

[Close]

BLAST of Lag0017486 vs. NCBI nr
Match: XP_038905834.1 (50S ribosomal protein L7/L12 [Benincasa hispida] >XP_038905841.1 50S ribosomal protein L7/L12 [Benincasa hispida])

HSP 1 Score: 323.2 bits (827), Expect = 1.6e-84
Identity = 168/191 (87.96%), Postives = 175/191 (91.62%), Query Frame = 0


Query: 4   IAVAARSLQVRPTLAKIIGLSQVRFFQPDFTPRDPNAKSKRYKYPAFYDPYGPRPSPSDK 63
           + V ARSLQVR T AKIIGLSQVRFFQ DF  RDPNAK KRYKYP FYDPYGPRP PSDK
Sbjct: 3   LTVVARSLQVRSTFAKIIGLSQVRFFQSDFALRDPNAKPKRYKYPPFYDPYGPRPPPSDK 62

Query: 64  IIQLAERIVALPVDERCQIGPTLSERLRHPKLQQISVDGLDMGSDGGAAAGSSKVEEIKE 123
           II+LAERI ALP ++RCQIGPTL ERL+HPKLQ ISVDGLDMGS+GGA AGSSKVEE KE
Sbjct: 63  IIELAERIAALPAEDRCQIGPTLRERLKHPKLQPISVDGLDMGSEGGAGAGSSKVEEKKE 122

Query: 124 KTAFDVKLEKFDAAAKIKVIKEVRSFTNLGLKEAKDLVEKVPAILKQGVTKEEANDIIEK 183
           KTAFDVKLEKFDAA+KIKVIKEVRSFTNLGLKEAKDLVEKVPAILKQGVTKEEANDIIEK
Sbjct: 123 KTAFDVKLEKFDAASKIKVIKEVRSFTNLGLKEAKDLVEKVPAILKQGVTKEEANDIIEK 182

Query: 184 IKAAGGVAVME 195
           IKAAGGVAVME
Sbjct: 183 IKAAGGVAVME 193

[Close]

BLAST of Lag0017486 vs. ExPASy Swiss-Prot
Match: Q2JM48 (50S ribosomal protein L7/L12 OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=rplL PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 6.1e-15
Identity = 50/129 (38.76%), Postives = 74/129 (57.36%), Query Frame = 0


Query: 60  PSDKIIQLAERIVALPVDERCQIGPTLSERLRHPKLQQISVDGLDMGSDGGAAAGSSKVE 119
           PS+++ ++ E + AL + E  ++   + E              +       AAA ++  E
Sbjct: 2   PSERVAKILEELKALSLLEASELVKAIEETFGVSAAAPAGGMVMAAPVAAAAAAPAAAPE 61

Query: 120 EIKEKTAFDVKLEKFDAAAKIKVIKEVRSFTNLGLKEAKDLVEKVPAILKQGVTKEEAND 179
            ++E+TAFDV LE   A  KI ++K VR  T LGLK+AKDLVE  P  +K+GV KEEAND
Sbjct: 62  PVEEQTAFDVILEAVPADKKIAILKVVRELTGLGLKDAKDLVEAAPKPVKEGVAKEEAND 121

Query: 180 IIEKIKAAG 189
           I +K++ AG
Sbjct: 122 IKKKLEEAG 130

[Close]

BLAST of Lag0017486 vs. ExPASy Swiss-Prot
Match: A2C031 (50S ribosomal protein L7/L12 OS=Prochlorococcus marinus (strain NATL1A) OX=167555 GN=rplL PE=3 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.4e-14
Identity = 44/85 (51.76%), Postives = 59/85 (69.41%), Query Frame = 0


Query: 110 GAAAGSSKVEEIKEKTAFDVKLEKFDAAAKIKVIKEVRSFTNLGLKEAKDLVEKVPAILK 169
           GAAAG    +  +EKT F+V LE FDA++KIKV+KEVR+ T LGL EAK LVE  P  +K
Sbjct: 47  GAAAGGDGADAAEEKTEFEVVLESFDASSKIKVLKEVRNATGLGLGEAKALVEAAPKTIK 106

Query: 170 QGVTKEEANDIIEKIKAAGGVAVME 195
           +G TKE+A  + + I+A GG   ++
Sbjct: 107 EGATKEDAEALKKAIEAVGGKVTLK 131

[Close]

BLAST of Lag0017486 vs. ExPASy Swiss-Prot
Match: A9BDG4 (50S ribosomal protein L7/L12 OS=Prochlorococcus marinus (strain MIT 9211) OX=93059 GN=rplL PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.8e-14
Identity = 45/93 (48.39%), Postives = 61/93 (65.59%), Query Frame = 0


Query: 102 GLDMGSDGGAAAGSSKVEEIKEKTAFDVKLEKFDAAAKIKVIKEVRSFTNLGLKEAKDLV 161
           G+ M + G     S+  E  +EKT FDV LE FDAAAKIKV+KEVR+ T LGL EAK +V
Sbjct: 40  GVVMAAPGAGGGASAAGEAAEEKTEFDVILESFDAAAKIKVLKEVRNATGLGLGEAKAMV 99

Query: 162 EKVPAILKQGVTKEEANDIIEKIKAAGGVAVME 195
           E  P  +K+G +KE+A  + + I+A GG   ++
Sbjct: 100 EAAPKTIKEGASKEDAEALKKAIEAVGGKVTLK 132

[Close]

BLAST of Lag0017486 vs. ExPASy Swiss-Prot
Match: P49550 (50S ribosomal protein L12, chloroplastic OS=Trieres chinensis OX=1514140 GN=rpl12 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.3e-14
Identity = 52/134 (38.81%), Postives = 76/134 (56.72%), Query Frame = 0


Query: 61  SDKIIQLAERIVALPVDERCQIGPTLSERLRHPKLQQISVDGLDMGSDGGAAAGSSKVEE 120
           S+KI Q+ E +  L + E  ++   + E           VD       G   AG + VEE
Sbjct: 2   SEKIDQIVEELKTLTLLEASELVSKIEE--------TFDVDASASVGGGMMMAGPAVVEE 61

Query: 121 IKEKTAFDVKLEKFDAAAKIKVIKEVRSFTNLGLKEAKDLVEKVPAILKQGVTKEEANDI 180
           ++EKT FDV L++  A  KI V+K VRS T LGLKEAK+LVE  P  +++GV K+ A + 
Sbjct: 62  VEEKTEFDVMLDEVPADKKIAVLKVVRSLTGLGLKEAKELVESAPKQIQEGVAKDAAEEA 121

Query: 181 IEKIKAAGGVAVME 195
            ++I+ AGG A ++
Sbjct: 122 KKQIEDAGGKASLK 127

[Close]

BLAST of Lag0017486 vs. ExPASy Swiss-Prot
Match: P41189 (50S ribosomal protein L7/L12 OS=Liberibacter africanus OX=34020 GN=rplL PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.3e-14
Identity = 43/83 (51.81%), Postives = 56/83 (67.47%), Query Frame = 0


Query: 111 AAAGSSKVEEIKEKTAFDVKLEKFDAAAKIKVIKEVRSFTNLGLKEAKDLVEKVPAILKQ 170
           A+A       + EKT F+V LE FDA  KI VIKEVR+ T LGLKEAKD VE  P  LK 
Sbjct: 42  ASAAGESAAAVAEKTEFEVFLEGFDAKKKISVIKEVRAITELGLKEAKDFVESAPKSLKT 101

Query: 171 GVTKEEANDIIEKIKAAGGVAVM 194
           GV+K+EA ++ +K++AAG   ++
Sbjct: 102 GVSKDEAEELKKKLEAAGATIIL 124

The following BLAST results are available for this feature:

BLAST of Lag0017486 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011651634.1	6.4e-86	88.48	uncharacterized protein LOC101206962 [Cucumis sativus] >XP_011651635.1 uncharact...	[more]
XP_022155073.1	1.4e-85	89.18	uncharacterized protein LOC111022207 [Momordica charantia] >XP_022155074.1 uncha...	[more]
XP_022949504.1	2.4e-85	88.14	uncharacterized protein LOC111452831 [Cucurbita moschata]	[more]
XP_023525921.1	7.1e-85	87.63	uncharacterized protein LOC111789392 [Cucurbita pepo subsp. pepo]	[more]
XP_038905834.1	1.6e-84	87.96	50S ribosomal protein L7/L12 [Benincasa hispida] >XP_038905841.1 50S ribosomal p...	[more]

BLAST of Lag0017486 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2JM48	6.1e-15	38.76	50S ribosomal protein L7/L12 OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=32...	[more]
A2C031	1.4e-14	51.76	50S ribosomal protein L7/L12 OS=Prochlorococcus marinus (strain NATL1A) OX=16755...	[more]
A9BDG4	1.8e-14	48.39	50S ribosomal protein L7/L12 OS=Prochlorococcus marinus (strain MIT 9211) OX=930...	[more]
P49550	2.3e-14	38.81	50S ribosomal protein L12, chloroplastic OS=Trieres chinensis OX=1514140 GN=rpl1...	[more]
P41189	2.3e-14	51.81	50S ribosomal protein L7/L12 OS=Liberibacter africanus OX=34020 GN=rplL PE=3 SV=...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014719	Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like	GENE3D	3.30.1390.10		coord: 120..193 e-value: 6.1E-23 score: 82.7
IPR014719	Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like	SUPERFAMILY	54736	ClpS-like	coord: 123..193
IPR013823	Ribosomal protein L7/L12, C-terminal	PFAM	PF00542	Ribosomal_L12	coord: 127..190 e-value: 3.2E-22 score: 78.7
IPR000206	Ribosomal protein L7/L12	PANTHER	PTHR45987	39S RIBOSOMAL PROTEIN L12	coord: 1..194
IPR000206	Ribosomal protein L7/L12	CDD	cd00387	Ribosomal_L7_L12	coord: 63..193 e-value: 1.5536E-37 score: 124.575
None	No IPR available	PANTHER	PTHR45987:SF6	RIBOSOMAL PROTEIN L12/ ATP-DEPENDENT CLP PROTEASE ADAPTOR PROTEIN CLPS FAMILY PROTEIN	coord: 1..194

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0017486.1	Lag0017486.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006412	translation
cellular_component	GO:0005840	ribosome
molecular_function	GO:0003735	structural constituent of ribosome